CN220829319U - PCR capillary leak tightness detection device - Google Patents
PCR capillary leak tightness detection device Download PDFInfo
- Publication number
- CN220829319U CN220829319U CN202322572636.0U CN202322572636U CN220829319U CN 220829319 U CN220829319 U CN 220829319U CN 202322572636 U CN202322572636 U CN 202322572636U CN 220829319 U CN220829319 U CN 220829319U
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- Prior art keywords
- detection
- pressurizing
- sliding table
- mounting plate
- assembly
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- 238000001514 detection method Methods 0.000 title claims abstract description 96
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 28
- 238000007789 sealing Methods 0.000 claims abstract description 22
- 238000006073 displacement reaction Methods 0.000 claims description 21
- 239000007788 liquid Substances 0.000 claims description 7
- 238000009434 installation Methods 0.000 claims description 5
- 238000010586 diagram Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007731 hot pressing Methods 0.000 description 1
- 210000001503 joint Anatomy 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000003566 sealing material Substances 0.000 description 1
- 210000003437 trachea Anatomy 0.000 description 1
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- Examining Or Testing Airtightness (AREA)
Abstract
The utility model discloses a PCR capillary leak tightness detection device, which comprises a bracket and a seal detection assembly fixed on the bracket, wherein the seal detection assembly comprises a seal block, a lifting pressurizing assembly and a lifting driving assembly; the lifting pressurizing assembly comprises a pressurizing mounting plate and one or more pressurizing connectors; the pressurizing mounting plate is driven by the lifting driving assembly to slide; the pressurizing connector is fixed on the pressurizing mounting plate; each pressurizing joint is connected to an air source through an air pipe; the sealing block is provided with detection holes corresponding to the number and the positions of the pressurizing connectors; the utility model pressurizes the PCR capillary tube to be detected inserted in the detection hole; if the joint of the PCR capillary tube has a sealing problem, gas leaks from the joint and is transmitted to the water tank through the detection hole and the detection air pipe to generate bubbles; so that a detector can intuitively observe whether the sealing of the connecting part of the PCR capillary to be detected is qualified.
Description
Technical Field
The utility model belongs to the technical field of capillary tube assembly, and particularly relates to a PCR capillary tube tightness detection device.
Background
The PCR capillary is a small tube body for carrying out micro PCR reaction (Polymerase Chain Reaction ), is suitable for the amplification reaction of micro samples, can save reagents and sample amount, and reduces the generation of wastes.
As shown in fig. 1, a PCR capillary for carrying a sample is generally composed of a capillary body 100 and a loading adapter 200. In the prior art, the capillary body 100 and the sample application port 200 are heated by a heat gun and then are fixedly connected by pressing. However, in the pressing process, the stress at the joint of the capillary main body and the sample adding joint is uneven due to external factors; thus, the partially processed PCR capillary tube has the liquid leakage phenomenon.
Therefore, the sealing detection is needed after the PCR capillary tube is processed; in the prior art, the mode of manually adding liquid into the processed PCR capillary and observing whether the liquid leakage phenomenon exists at the joint is low in efficiency and has the risk of error. Therefore, it is necessary to provide a detection device capable of automatically detecting the tightness of a PCR capillary to be tested and visually detecting whether the tightness of the PCR capillary to be tested is acceptable or not by a worker.
Disclosure of Invention
The utility model aims to provide a PCR capillary leak tightness detection device.
The utility model provides a PCR capillary leak tightness detection device, which comprises a bracket and a seal detection assembly fixed on the bracket, wherein the seal detection assembly comprises a detection mounting block, a lifting pressurizing assembly and a lifting driving assembly; the lifting pressurizing assembly comprises a pressurizing mounting plate and one or more pressurizing connectors; the pressurizing mounting plate is driven by the lifting driving assembly to slide; the pressurizing connector is fixed on the pressurizing mounting plate; each pressurizing joint is connected to an air source through an air pipe;
The detection mounting block is provided with detection holes corresponding to the number and the positions of the pressurizing connectors; one end of the detection air pipe is communicated with the detection hole; the other end of the detection air pipe extends into the water tank. The junction of the capillary body and the sample adding connector in the PCR capillary to be tested can be inserted into the detection hole; and the top of detecting the installation piece can provide the support for the step face on the application of sample joint.
Preferably, the top of the water tank is arranged in an open mode; the water tank is filled with water, and the end part of the detection air pipe is immersed below the liquid level.
Preferably, the detection hole penetrates through the sealing block; the detection air pipe is connected with the bottom end opening of the detection hole; the aperture of the detection hole is larger than the diameter of the capillary body of the PCR capillary to be detected and smaller than the diameter of the sample adding connector.
Preferably, the seal detection assembly further comprises an image acquisition device; the image acquisition device is located directly over the water tank, and the acquisition end is right opposite to the water tank.
Preferably, a sealing block (2-2) corresponding to the position of the detection hole (2-1-1) is fixed on the detection mounting block (2-1). The top end of the detection hole (2-1-1) penetrates through the sealing block (2-2).
Preferably, the apparatus further comprises a displacement transport assembly; the displacement transportation assembly comprises a two-shaft sliding table, a sucker and a displacement mounting plate arranged at the tail end of the two-shaft sliding table; the two-axis sliding table is used for driving the displacement mounting plate to move in two degrees of freedom; the sucking disc is installed on the displacement mounting plate.
Preferably, the two-axis sliding table comprises a transverse sliding table and a vertical sliding table. The transverse sliding table is arranged on the bracket; the vertical slipway is installed in the removal portion of sideslip slipway. The displacement mounting plate is fixed with the moving part of the vertical sliding table.
Preferably, the transverse sliding table and the vertical sliding table both adopt air cylinders.
Preferably, the moving direction of the pressurizing mounting plate driven by the lifting driving assembly is parallel to the central axis of the detection hole; and the central axis of the pressurizing connector coincides with the central axis of the detection hole.
Preferably, the lifting driving assembly adopts an air cylinder.
The utility model has the beneficial effects that:
The utility model is through setting up the correspondent detection hole and pressurized joint of position, and connect the inner chamber of the detection hole to the water tank through detecting the air pipe; when the PCR capillary tube with the sealing defect is inserted into the detection hole and pressurized to the inner cavity of the PCR capillary tube through the pressurizing connector; the gas input into the PCR capillary leaks from the joint of the capillary main body and the sample adding connector, and is transmitted into the water tank through the detection hole and the detection air pipe to generate bubbles; so that a inspector or a camera can intuitively observe whether the tightness of the PCR capillary tube is qualified or not.
Drawings
FIG. 1 is a PCR capillary finished by processing in the prior art;
FIG. 2 is a schematic diagram of the overall structure of the present utility model;
FIG. 3 is a schematic diagram showing the relative positions of the lifting and pressurizing assembly and the detecting and mounting block in the present utility model;
FIG. 4 is a schematic diagram showing the relative positions of a PCR capillary and a seal detection site in the detection process of the present utility model.
100 Parts of capillary main body; 200. a sample addition joint; 1. a bracket; 2. a seal detection assembly; 2-1, detecting an installation block; 2-2, a sealing block; 2-3, detecting a trachea; 2-4, a water tank; 2-5, a camera; 2-6, lifting and pressurizing components; 2-1-1, sealing detection bits; 2-6-1, pressurized joint; 2-6-2, a pressurizing mounting plate; 2-7, lifting driving components; 3. a displacement transport assembly; 3-1, a two-axis sliding table; 3-2, displacement mounting plates; 3-3, sucking disc.
Detailed Description
The utility model is further described below with reference to the accompanying drawings.
As shown in fig. 2, a PCR capillary leak tightness detection device includes a bracket 1, and a leak tightness detection assembly 2 and a displacement transport assembly 3 mounted on the bracket 1. The displacement transportation assembly 3 comprises a two-axis sliding table 3-1, a driving element, a sucker 3-3 and a displacement mounting plate 3-2 arranged at the tail end of the two-axis sliding table 3-1. The two-axis sliding table 3-1 comprises a transverse sliding table and a vertical sliding table. The transverse sliding table is arranged on the bracket; the vertical slipway is installed in the removal portion of sideslip slipway. The displacement mounting plate 3-2 is fixed with the moving part of the vertical sliding table. The transverse sliding table and the vertical sliding table both adopt air cylinders, and can drive the displacement mounting plate 3-2 to move along the vertical and horizontal directions in two degrees of freedom. The sucking disc 3-3 is arranged on the bottom surface of the displacement mounting plate 3-2 and is used for sucking the PCR capillary tube to be detected and transporting the PCR capillary tube to be detected to the seal detection assembly 2.
The seal detection assembly 2 comprises a detection installation block 2-1, a seal block 2-2, a detection air pipe 2-3, a water tank 2-4, an image acquisition device, a lifting pressurization assembly 2-6 and a lifting driving assembly 2-7. The detection installation block 2-1, the water tank 2-4 and the image acquisition device are all fixed on the bracket 1. The top of the water tank 2-4 is arranged in an open mode. The image acquisition device adopts cameras 2-5. The camera 2-5 is positioned right above the water tank 2-4, and the shooting end of the camera 2-5 is right opposite to the water tank 2-4. The camera 2-5 is used for shooting images of the water tank 2-4 and transmitting the images to the upper computer, and judging whether bubbles appear in the water tank 2-4.
The sealing block 2-2 is embedded and fixed on the detection mounting block 2-1. Two detection holes 2-1-1 are formed in the detection mounting block 2-1 and the sealing block 2-2; the detection hole 2-1-1 penetrates through the detection mounting block 2-1 and the sealing block 2-2; the aperture of the detection hole 2-1-1 is larger than the diameter of the capillary body 100 of the PCR capillary to be detected and smaller than the diameter of the sample adding joint; so that the capillary tube formed by the hot pressing can be inserted into the detection hole. The sealing block 2-2 is a rubber block; the top opening of the detection hole 2-1-1 is sealed by matching the step surface of the PCR capillary after the PCR capillary is inserted into the detection hole 2-1-1. Simultaneously, the top surface of the sealing block 2-2 is used for supporting the sample feeding suction head; ensuring that the position of the PCR capillary to be detected is stable in the detection process. Each detection hole is correspondingly provided with a detection air pipe 2-3. One end of the detection air pipe 2-3 is connected with the bottom end opening of the detection hole on the sealing block 2-2; the other end of the detection air pipe 2-3 extends into the water tank 2-4, and the port is immersed below the liquid level. During detection, the PCR capillary to be detected is inserted into the detection hole, so that the detection hole is closed at the top end and open at the bottom end.
In some embodiments, the test mounting block 2-1 is not provided with a sealing block, but the entire test mounting block 2-1 is made of an elastic sealing material.
As shown in FIG. 3, the lifting and pressurizing assembly 2-6 includes a pressurizing connector 2-6-1 and a pressurizing mounting plate 2-6-2. The pressurizing mounting plate 2-6-2 is driven by the lifting driving component 2-7 to slide; the lifting driving component 2-7 adopts an air cylinder. The pressurizing mounting plate 2-6-2 is fixedly provided with pressurizing connectors 2-6-1 corresponding to the number of the detecting holes. The pressurizing connectors 2-6-1 are positioned right above the detection holes, and the axis of each pressurizing connector 2-6-1 coincides with the axis of the corresponding detection hole. Each pressurizing connector 2-6-1 is connected to a gas source through a gas pipe.
As shown in fig. 3 and 4, during the detection, the water tank 2-4 is filled with water, and the end of the detection air pipe is immersed below the liquid level; the displacement transport assembly 3 transfers the PCR capillary tube to be detected to the detection hole through the sucker 3-3; the junction of the sample-adding connector 200 and the capillary body 100 in the PCR capillary to be measured extends into the detection hole. The pressurizing connector 2-6-1 of the lifting pressurizing component 2-6 is in butt joint with the sample adding connector 200 on the PCR capillary tube and pressurizes the PCR capillary tube; if there is a sealing defect at the junction between the capillary body 100 and the sample application adapter 200 in the PCR capillary, the gas inputted into the PCR capillary leaks out from the junction and is transferred into the water tank 2-4 through the detection hole and the detection gas pipe 2-3 to generate bubbles.
And shooting images of the water tanks 2-4 through the cameras 2-5, and uploading the shot images to an upper computer. The upper computer automatically recognizes whether bubbles exist in the image shot by the camera 2-5 by using an image recognition algorithm, and judges whether the PCR capillary is leaked or not according to the bubbles.
Claims (10)
1. The utility model provides a PCR capillary leak tightness detection device, includes support (1) to and fix seal detection subassembly (2) on support (1), its characterized in that: the sealing detection assembly (2) comprises a detection installation block (2-1), a lifting pressurizing assembly (2-6) and a lifting driving assembly (2-7); the lifting pressurizing assembly (2-6) comprises a pressurizing mounting plate (2-6-2) and one or more pressurizing connectors (2-6-1); the pressurizing mounting plate (2-6-2) is driven by the lifting driving component (2-7) to slide; the pressurizing connector (2-6-1) is fixed on the pressurizing mounting plate (2-6-2); each pressurizing joint (2-6-1) is connected to an air source through an air pipe;
The detection mounting block (2-1) is provided with detection holes (2-1-1) corresponding to the number and the positions of the pressurizing connectors (2-6-1); one end of the detection air pipe (2-3) is communicated with the detection hole (2-1-1); the other end of the detection air pipe (2-3) extends into the water tank (2-4).
2. The PCR capillary leak tightness testing device as set forth in claim 1, wherein: the top of the water tank (2-4) is arranged in an open mode; the water tank (2-4) is filled with water, and the end part of the detection air pipe (2-3) is immersed below the liquid level.
3. The PCR capillary leak tightness testing device as set forth in claim 1, wherein: the detection hole (2-1-1) penetrates through the sealing block (2-2); the detection air pipe (2-3) is connected with the bottom end opening of the detection hole (2-1-1); the aperture of the detection hole is larger than the diameter of the capillary body (100) of the PCR capillary to be detected and smaller than the diameter of the sample adding connector.
4. The PCR capillary leak tightness testing device as set forth in claim 1, wherein: the seal detection assembly (2) further comprises an image acquisition device; the image acquisition device is positioned right above the water tank (2-4), and the acquisition end is right opposite to the water tank (2-4).
5. The PCR capillary leak tightness testing device as set forth in claim 1, wherein: the detection mounting block (2-1) is fixedly provided with a sealing block (2-2) corresponding to the detection hole (2-1-1), and the top end of the detection hole (2-1-1) penetrates through the sealing block (2-2).
6. The PCR capillary leak tightness testing device as set forth in claim 1, wherein: further comprises a displacement transportation assembly (3); the displacement transportation assembly (3) comprises a two-axis sliding table (3-1), a sucker (3-3) and a displacement mounting plate (3-2) arranged at the tail end of the two-axis sliding table (3-1); the two-axis sliding table (3-1) is used for driving the displacement mounting plate (3-2) to move in two degrees of freedom; the sucker (3-3) is arranged on the displacement mounting plate (3-2).
7. The PCR capillary leak tightness testing device as set forth in claim 6, wherein: the two-axis sliding table (3-1) comprises a transverse sliding table and a vertical sliding table; the transverse sliding table is arranged on the bracket; the vertical sliding table is arranged on the moving part of the transverse sliding table; the displacement mounting plate (3-2) is fixed with the moving part of the vertical sliding table.
8. The PCR capillary leak tightness testing device as set forth in claim 7, wherein: and the transverse sliding table and the vertical sliding table both adopt air cylinders.
9. The PCR capillary leak tightness testing device as set forth in claim 6, wherein: the moving direction of the pressurizing mounting plate (2-6-2) driven by the lifting driving assembly is parallel to the central axis of the detection hole; and the central axis of the pressurizing connector (2-6-1) coincides with the central axis of the detection hole.
10. The PCR capillary leak tightness testing device as set forth in claim 6, wherein: the lifting driving assembly (2-7) adopts an air cylinder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322572636.0U CN220829319U (en) | 2023-09-21 | 2023-09-21 | PCR capillary leak tightness detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322572636.0U CN220829319U (en) | 2023-09-21 | 2023-09-21 | PCR capillary leak tightness detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220829319U true CN220829319U (en) | 2024-04-23 |
Family
ID=90724639
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322572636.0U Active CN220829319U (en) | 2023-09-21 | 2023-09-21 | PCR capillary leak tightness detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220829319U (en) |
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2023
- 2023-09-21 CN CN202322572636.0U patent/CN220829319U/en active Active
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