CN220788632U - Cell balling experimental device - Google Patents
Cell balling experimental device Download PDFInfo
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- CN220788632U CN220788632U CN202322447040.8U CN202322447040U CN220788632U CN 220788632 U CN220788632 U CN 220788632U CN 202322447040 U CN202322447040 U CN 202322447040U CN 220788632 U CN220788632 U CN 220788632U
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- shell
- culture dish
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- 230000007246 mechanism Effects 0.000 claims abstract description 13
- 230000005540 biological transmission Effects 0.000 claims description 19
- 238000007789 sealing Methods 0.000 claims description 3
- 238000003556 assay Methods 0.000 claims 5
- 239000001963 growth medium Substances 0.000 abstract description 12
- 230000008859 change Effects 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 description 15
- 210000004027 cell Anatomy 0.000 description 9
- 238000000034 method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell balling experimental device, which comprises a culture dish provided with a plurality of culture holes, wherein the culture dish is arranged in a shell, an opening is formed in the top of the shell, a cover plate is arranged in the opening, the cover plate is positioned between the top plate and the culture dish, an operation hole is formed in the cover plate, and the position of the operation hole corresponds to the position of the culture hole; the shell is detachably connected to the base through a connecting piece, a driving mechanism is arranged on the base, and the driving mechanism drives the culture dish to rotate in the shell; this cell balling experimental apparatus, the operation hole on the guard plate corresponds with cultivateing the hole, makes the culture dish rotate in the shell through actuating mechanism's operation, and the rotation of culture dish makes the cultivation hole of different positions correspond the operation hole in proper order, and the experimenter of being convenient for just can be comprehensive from an operation hole department to change and add the culture medium in any one cultivation hole.
Description
Technical Field
The utility model belongs to the technical field of cell experiments, and particularly relates to a cell balling experimental device.
Background
The balling capacity is an important method for identifying tumor stem cells in vitro, and is judged to be the capacity of single cells to self-renew under a proper condition culture medium, the balling culture medium needs to be prepared in the cell balling experiment process, a proper culture dish is needed to be used, the culture medium is added into the culture dish twice, the first culture medium needs to be taken out when the culture medium is added into the culture dish for the second time, generally, the experiment personnel needs to manually operate, in order to avoid pollution, the experiment personnel firstly opens the cover of the culture dish through the left hand, forms an angle, is not completely opened, is properly inclined, sucks old liquid by a suction pipe, and shakes the culture dish after adding new liquid, so that the new liquid is uniformly distributed, in the process, the operation difficulty of the experiment personnel is increased because the cover is not completely opened as much as possible, old liquid in the culture hole of the culture dish in some positions is not completely taken out, the new liquid can not be uniformly distributed, or the cover of the culture dish is completely opened, and the new liquid is replaced, and the risk of pollution to the outside is increased.
Disclosure of Invention
The utility model aims to: the utility model provides a cell balling experimental apparatus to solve among the prior art the operation degree of difficulty that laboratory personnel changed the culture medium is big, and also very easy the result in the old liquid in the culture hole of culture dish in some positions not taken out completely, and new liquid also probably can not even distribute, and if directly open the lid of culture dish completely, replace new old liquid again, this kind of operation has increased the problem by the risk of external pollution.
The technical scheme is as follows:
the cell balling experimental device comprises a round culture dish with a plurality of culture holes formed at equal angles, wherein the culture dish is arranged in a shell, an opening is formed in the top of the shell, a cover plate is arranged in the opening, the cover plate comprises a protection plate and a top plate and is used for opening and closing the opening, the protection plate is positioned between the top plate and the culture dish, an operation hole is formed in the protection plate, and the position of the operation hole corresponds to the position of the culture hole;
the shell is detachably connected to the base through the connecting piece, a driving mechanism is arranged on the base and drives the culture dish to rotate in the shell, and the shell is in sliding connection with the culture dish through the sliding piece.
In a further embodiment, the circle centers of the operation holes are the same as the circle centers of the culture holes, and the diameter of the operation holes is smaller than that of the culture holes.
In a further embodiment, the slider comprises a slider mounted on the outer wall of the culture dish, the slider sliding in an annular chute, the chute being provided on the inner wall of the housing.
In a further embodiment, the protection plate is hinged with the shell, the top plate is hinged with the shell, and sealing layers are arranged at the positions of the top plate and the protection plate, which are in contact with the shell; the opening of the opening is not required to be completely opened, and the operation is only required to be performed through the operation hole.
In a further embodiment, the drive mechanism comprises a motor mounted on the base and a transmission member comprising two intermeshing gears, one of which is mounted on the output of the motor and the other of which is mounted on a drive shaft;
the bottom of the transmission shaft is rotationally connected to the base, the top of the transmission shaft is matched and inserted into a connecting groove formed in the connecting shaft, the top of the connecting shaft is connected to the center of the bottom of the culture dish, and the bottom of the transmission shaft is rotationally connected to the shell; can be convenient for the rotation regulation of culture dish.
In a further embodiment, the connecting piece comprises a plug rod, the top end of the plug rod is connected to the shell, the bottom end of the plug rod is inserted into a slot, and the slot is formed in the base; can be convenient for the accurate and stable connection of shell on the base.
The utility model has the beneficial effects that: through locating the culture dish in the shell, can be further protect the culture dish, and when needing to change old and new liquid, open the roof, the operation hole on the guard plate corresponds with the culture hole, the operation through actuating mechanism makes the culture dish rotate in the shell, the rotation of culture dish makes the culture hole of different positions correspond the operation hole in proper order, the experimenter of being convenient for only from an operation hole department just can be comprehensive change and add the culture medium in any one culture hole, the effectual old liquid that avoids draws incompletely, the inhomogeneous condition of new liquid addition appears, and the experimenter also need not to last manual to adjust the culture dish, the operation degree of difficulty has been reduced, the inside and the too many possible risks that produce of external contact of culture dish have also been reduced simultaneously, more do benefit to the going on of cell balling experiment.
Drawings
Fig. 1 is a schematic view of the front cross-sectional structure of the present utility model.
Fig. 2 is an enlarged schematic view of the structure of fig. 1 a according to the present utility model.
FIG. 3 is a schematic perspective view of the guard plate and culture dish according to the utility model.
The reference numerals are: the culture dish 1, the shell 2, the sliding piece 3, the sliding block 31, the sliding groove 32, the cover plate 4, the protection plate 41, the operation hole 411, the top plate 42, the connecting piece 5, the base 6, the driving mechanism 7, the motor 71, the gear 72, the transmission shaft 73 and the connecting shaft 74.
Detailed Description
In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present utility model. It will be apparent, however, to one skilled in the art that the utility model may be practiced without one or more of these details. In other instances, well-known features have not been described in detail in order to avoid obscuring the utility model.
The present utility model will be described in further detail with reference to the accompanying drawings.
Referring to fig. 1-3, a cell balling experimental device disclosed by the utility model comprises a round culture dish 1 with a plurality of culture holes at equal angles, wherein the culture dish 1 can be 6, 12, 24 pore plates or 100mm, the culture dish 1 is arranged in a shell 2, an opening is arranged at the top of the shell 2, a cover plate 4 is arranged in the opening, the cover plate 4 comprises a protection plate 41 and a top plate 42 for opening and closing the opening, the protection plate 41 is positioned between the top plate 42 and the culture dish 1, an operation hole 411 is formed in the protection plate 41, and the position of the operation hole 411 corresponds to the position of the culture hole; the shell 2 is detachably connected to the base 6 through the connecting piece 5, a driving mechanism 7 is arranged on the base 6, the driving mechanism 7 drives the culture dish 1 to rotate in the shell 2, and the shell 2 is in sliding connection with the culture dish 1 through the sliding piece 3.
The centre of a circle of operation hole 411 is the same with the centre of a circle of culture hole that is located under it, the diameter of operation hole 411 is less than the diameter of culture hole, when actuating mechanism 7 drive culture dish 1 rotates, every culture hole can all be located the below of operation hole 411 in proper order to the laboratory glassware of being convenient for uses the straw to pass operation hole 411 earlier and get into the culture hole of corresponding position and draw old liquid, and the new culture medium of interpolation is in the culture hole that changes to rotating in proper order to change the burette through operation hole 411 again, and the setting of diameter size can avoid operation hole 411 too big to lead to cultivateing Kong Guoduo's contact external world, also effectually has limited straw and burette simultaneously.
The sliding part 3 comprises a sliding block 31 arranged on the outer wall of the culture dish 1, the sliding block 31 slides in an annular sliding groove 32, the sliding groove 32 is arranged on the inner wall of the shell 2, the culture dish 1 is stably connected with the shell 2 through the sliding part 3, and meanwhile, the culture dish can slide with the sliding groove 32 through the sliding block 31.
The guard plate 41 is hinged to the housing 2, the top plate 42 is hinged to the housing 2, sealing layers are arranged at the positions where the top plate 42 and the guard plate 41 are in contact with the housing 2, when the operation hole 411 is used, the top plate 42 is turned over firstly, so that the guard plate 41 still covers the culture dish 1 at the opening, only the operation hole 411 is exposed, and when the culture operation needs to be continued, the top plate 42 is turned back to close the opening and the operation hole 411, preferably, the guard plate 41 can also be hinged to the top plate 42; preferably, the protection plate 41 may also be in a separated structure with the top plate 42, and the protection plate 41 may be supported below the top plate 42 and below the culture dish 1 at the opening by a support member, so that the protection plate 41 needs to be ensured to cover the opening and the position of the operation hole 411 is suitable, and meanwhile, the opening and closing of the top plate 42 are not affected.
The driving mechanism 7 comprises a motor 71 mounted on the base 6 and a transmission member comprising two gears 72 meshed with each other, wherein one gear 72 is mounted on the output end of the motor 71, and the other gear 72 is mounted on a transmission shaft 73; the bottom of the transmission shaft 73 is rotatably connected to the base 6, the top of the transmission shaft 73 is matched with the transmission shaft to be inserted into a connecting groove formed in the connecting shaft 74, the top of the connecting shaft 74 is connected to the bottom center position of the culture dish 1, the bottom of the transmission shaft 74 is rotatably connected to the casing 2, when the casing 2 is connected with the base 6 from top to bottom through the connecting piece 5, the position of the connecting shaft 74 corresponds to the transmission shaft 73 until the transmission shaft 73 can be inserted into the connecting groove, and when the motor 71 runs, the culture dish 1 is rotated through the rotation of the gear 72, so that culture holes at different positions can sequentially correspond to the operation holes 411.
The connecting piece 5 comprises a plug rod, the top end of the plug rod is connected to the shell 2, the bottom end of the plug rod is inserted into a slot, the slot is formed in the base 6, the shell 2 can be limited above the base 6 by being inserted into the slot through the plug rod in a matched mode, and the connecting shaft 74 is connected with the transmission shaft 73 in a matched mode.
The implementation principle of the utility model is as follows: the stem cells and the culture medium are added into the culture holes, the cover plate 4 is used for closing the opening to culture under a proper temperature environment, the culture medium is completely added twice a week, when the culture medium needs to be replaced, the shell 2 is connected to the base 6 through the connecting piece 5, the transmission shaft 73 is matched with the connecting shaft 74, the operation holes 411 are exposed after the top plate 42 is opened, the motor 71 is opened again, the culture holes at different positions can be matched with the operation holes 411, the replacement of new and old liquid is facilitated, and the old liquid in all the culture holes can be taken out first and then is sequentially added into the culture holes.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
The preferred embodiments of the present utility model have been described in detail above with reference to the accompanying drawings, but the present utility model is not limited to the specific details of the above embodiments, and various equivalent changes can be made to the technical solutions of the present utility model within the scope of the technical concept of the present utility model, and these equivalent changes all fall within the scope of the present utility model.
Claims (6)
1. Cell balling experimental apparatus, including equiangular round culture dish that has seted up a plurality of culture holes, its characterized in that: the culture dish is arranged in the shell, an opening is formed in the top of the shell, a cover plate is arranged in the opening, the cover plate comprises a protection plate and a top plate and is used for opening and closing the opening, the protection plate is positioned between the top plate and the culture dish, an operation hole is formed in the protection plate, and the position of the operation hole corresponds to the position of the culture hole;
the shell is detachably connected to the base through the connecting piece, a driving mechanism is arranged on the base and drives the culture dish to rotate in the shell, and the shell is in sliding connection with the culture dish through the sliding piece.
2. The cell balling assay device of claim 1, wherein: the circle center of the operation hole is the same as that of the culture hole, and the diameter of the operation hole is smaller than that of the culture hole.
3. The cell balling assay device of claim 1, wherein: the sliding piece comprises a sliding block arranged on the outer wall of the culture dish, the sliding block slides in an annular sliding groove, and the sliding groove is formed in the inner wall of the shell.
4. The cell balling assay device of claim 1, wherein: the guard plate with the shell is articulated, the roof with the guard plate with the position that the shell contacted all is equipped with the sealing layer.
5. The cell balling assay device of claim 1, wherein: the driving mechanism comprises a motor and a transmission part which are arranged on the base, wherein the transmission part comprises two gears which are meshed with each other, one gear is arranged on the output end of the motor, and the other gear is arranged on the transmission shaft;
the bottom of transmission shaft rotates to be connected on the base, and the top cooperation is inserted in the spread groove of seting up in the connecting axle, the top of connecting axle is connected the bottom central point of culture dish puts, and the bottom rotates to be connected in the shell.
6. The cell balling assay device of claim 1, wherein: the connecting piece comprises an inserting rod, the top end of the inserting rod is connected to the shell, the bottom end of the inserting rod is inserted into the slot, and the slot is formed in the base.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322447040.8U CN220788632U (en) | 2023-09-08 | 2023-09-08 | Cell balling experimental device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322447040.8U CN220788632U (en) | 2023-09-08 | 2023-09-08 | Cell balling experimental device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220788632U true CN220788632U (en) | 2024-04-16 |
Family
ID=90636056
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322447040.8U Active CN220788632U (en) | 2023-09-08 | 2023-09-08 | Cell balling experimental device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220788632U (en) |
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2023
- 2023-09-08 CN CN202322447040.8U patent/CN220788632U/en active Active
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