CN220730039U - Flow cell for simultaneously measuring ultraviolet-visible absorption and fluorescence spectrum - Google Patents
Flow cell for simultaneously measuring ultraviolet-visible absorption and fluorescence spectrum Download PDFInfo
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- CN220730039U CN220730039U CN202322251290.4U CN202322251290U CN220730039U CN 220730039 U CN220730039 U CN 220730039U CN 202322251290 U CN202322251290 U CN 202322251290U CN 220730039 U CN220730039 U CN 220730039U
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- 238000002189 fluorescence spectrum Methods 0.000 title claims abstract description 56
- 238000000862 absorption spectrum Methods 0.000 title claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 140
- 238000001514 detection method Methods 0.000 claims abstract description 41
- 210000004027 cell Anatomy 0.000 claims abstract description 22
- 210000005056 cell body Anatomy 0.000 claims abstract description 17
- 239000000463 material Substances 0.000 claims abstract description 6
- 230000000903 blocking effect Effects 0.000 claims abstract description 5
- 238000005259 measurement Methods 0.000 claims description 15
- 238000002371 ultraviolet--visible spectrum Methods 0.000 claims 9
- 238000010521 absorption reaction Methods 0.000 description 8
- 238000001917 fluorescence detection Methods 0.000 description 5
- 238000001228 spectrum Methods 0.000 description 5
- 238000007792 addition Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Optical Measuring Cells (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The utility model discloses a flow cell for simultaneously measuring ultraviolet-visible absorption and fluorescence spectrum, which comprises a cell body, wherein a liquid flow path is arranged in the cell body, and a liquid inlet and a liquid outlet which are communicated with the liquid flow path are also arranged on the cell body; the liquid flow path comprises a first liquid flow path and a second liquid flow path, an ultraviolet visible light incident hole and an ultraviolet visible light detection hole are arranged on the tank body perpendicular to the direction of the first liquid flow path, and the ultraviolet visible light incident hole and the ultraviolet visible light detection hole are oppositely arranged; the cell body is provided with a fluorescence spectrum light incident hole and a fluorescence spectrum light detection hole perpendicular to the direction of the second liquid flow path, and the fluorescence spectrum light incident hole and the fluorescence spectrum light detection hole are distributed in a vertical right angle. The liquid to be detected is divided into two parts, and is blocked by the light blocking material, so that ultraviolet-visible absorption spectrum and fluorescence spectrum detection are respectively carried out, and ultraviolet-visible absorption and fluorescence spectrum simultaneous detection can be realized.
Description
Technical Field
The utility model belongs to the technical field of chromatography, and particularly relates to a flow cell for simultaneously measuring ultraviolet-visible absorption and fluorescence spectrum.
Background
The liquid chromatograph is an instrument for liquid identification, and ultraviolet-visible absorption detection and fluorescence detection are the most widely used detection means of the liquid chromatograph. The ultraviolet-visible absorption detection uses a broadband light source to measure the transmittance of different wavelengths of light beams after the light beams penetrate through the liquid, and is used for analyzing the components, the content and the structure of the liquid. Fluorescence detection uses a single wavelength laser to collect fluorescence signals excited in a liquid for detection, and is used for analyzing the structural characteristics of detected atoms or molecules.
In the practical use of the liquid chromatograph, the combined detection of ultraviolet-scientific absorption and fluorescence detection is realized on the same instrument, so that the use simplicity can be improved, and the cost can be reduced. At present, the scheme for realizing the combined detection of ultraviolet-scientific absorption and fluorescence detection mainly adopts a T-shaped light path design, the incident light of ultraviolet-visible absorption is opposite to a detection end, the excitation light of fluorescence is vertically incident, and the ultraviolet-visible absorption and the detection end share a detection spectrum instrument. However, in this scheme, the ultraviolet-visible absorption irradiates the same sample area as the fluorescence detection, and the signals of the two interfere with each other, so that simultaneous measurement is not possible.
Disclosure of Invention
The utility model mainly aims to provide a flow cell for simultaneously measuring ultraviolet-visible absorption and fluorescence spectrum so as to overcome the defects in the prior art.
In order to achieve the above purpose, the technical scheme adopted by the utility model comprises the following steps:
the utility model provides a flow cell for simultaneously measuring ultraviolet-visible absorption and fluorescence spectrum, which comprises a cell body, wherein a liquid flow path is arranged in the cell body, and a liquid inlet and a liquid outlet which are communicated with the liquid flow path are also arranged on the cell body; the liquid flow paths comprise a first liquid flow path and a second liquid flow path, an ultraviolet visible light incident hole and an ultraviolet visible light detection hole are formed in the tank body perpendicular to the direction of the first liquid flow path, and the ultraviolet visible light incident hole and the ultraviolet visible light detection hole are oppositely arranged; and a fluorescence spectrum light incident hole and a fluorescence spectrum light detection hole are arranged on the tank body in the direction perpendicular to the second liquid flow path, and the fluorescence spectrum light incident hole and the fluorescence spectrum light detection hole are distributed in a vertical right angle.
Further, the parts except the ultraviolet visible light incidence hole, the ultraviolet visible light detection hole, the fluorescence spectrum light incidence hole and the fluorescence spectrum light detection hole are filled with light blocking materials.
Further, the liquid inlet and the liquid outlet on the tank body are respectively arranged on two parallel side surfaces on the tank body, and two ends of the first liquid flow path and the second liquid flow path are respectively communicated with the liquid inlet and the liquid outlet.
Further, the first liquid flow path and the second liquid flow path are symmetrically arranged along the connecting line of the liquid inlet and the liquid outlet.
Further, the liquid flow path is divided into two parts from the liquid inlet, and extends along the first liquid flow path and the second liquid flow path to be converged to the liquid outlet.
Furthermore, the liquid inlet is provided with a liquid inflow guide tip face, and the liquid outlet is provided with a liquid outflow guide tip face.
Further, the liquid inlet and the liquid outlet on the tank body are arranged on the same side face of the tank body, one ends of the first liquid flow path and the second liquid flow path are communicated to form a closed state, and the other ends of the first liquid flow path and the second liquid flow path are respectively communicated with the liquid inlet and the liquid outlet.
Further, the liquid flow path is communicated with the liquid outlet from the liquid inlet to form a U-shaped structure.
Further, the light source emitted by the ultraviolet visible light incident hole adopts a broadband light source.
Further, the light source emitted by the fluorescence spectrum light incident hole adopts a single-wavelength laser light source.
Compared with the prior art, the utility model has the following beneficial effects:
the flow cell for simultaneously measuring the ultraviolet-visible absorption and fluorescence spectrum can divide the liquid to be measured into two parts, uses the light blocking material to separate the liquid to be measured, respectively detects the ultraviolet-visible absorption spectrum and the fluorescence spectrum, and adopts a mode of respectively detecting, thereby avoiding the phenomena of overexposure or excessively low signal possibly occurring when the detector switches the measuring mode.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present application, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a top view of a flow cell for simultaneous measurement of UV-visible absorption and fluorescence spectra in example 1 of the present application.
FIG. 2 is a front view of a flow cell for simultaneous measurement of UV-visible absorption and fluorescence spectra in example 1 of the present application.
FIG. 3 is a right side view of a flow cell for simultaneous measurement of UV-visible absorption and fluorescence spectra in example 1 of the present application.
FIG. 4 is a top view of a flow cell for simultaneous measurement of UV-visible absorption and fluorescence spectra in example 2 of the present application.
Reference numerals illustrate: 1. the device comprises a liquid inlet, a liquid outlet, an ultraviolet visible light inlet hole, a fluorescent spectrum light inlet hole, an ultraviolet visible light detection hole, a fluorescent spectrum light detection hole and a tank body.
Detailed Description
The utility model will be more fully understood from the following detailed description, which should be read in conjunction with the accompanying drawings. Detailed embodiments of the present utility model are disclosed herein; however, it is to be understood that the disclosed embodiments are merely exemplary of the utility model, which may be embodied in various forms. Therefore, specific functional details disclosed herein are not to be interpreted as limiting, but merely as a basis for the claims and as a representative basis for teaching one skilled in the art to variously employ the present utility model in virtually any appropriately detailed embodiment.
Example 1
The embodiment provides a flow cell for simultaneously measuring ultraviolet-visible absorption and fluorescence spectrum, which is shown in figures 1-3, and comprises a cell body 7, wherein a liquid flow path is arranged in the cell body 7, and a liquid inlet 1 and a liquid outlet 2 which are communicated with the liquid flow path are also arranged on the cell body 7; the liquid flow paths comprise a first liquid flow path and a second liquid flow path, the liquid inlet 1 and the liquid outlet 2 on the tank body 7 are respectively arranged on two parallel side surfaces on the tank body 7, two ends of the first liquid flow path and the second liquid flow path are respectively communicated with the liquid inlet 1 and the liquid outlet 2, and the first liquid flow path and the second liquid flow path are symmetrically arranged along the connecting line of the liquid inlet 1 and the liquid outlet 2.
In this embodiment, the liquid flow path is divided into two parts from the liquid inlet 1, and extends and converges to the liquid outlet 2 along the first liquid flow path and the second liquid flow path respectively, more specifically, the liquid inlet 1 is provided with a liquid inflow guide tip surface, the liquid outlet 2 is provided with a liquid outflow guide tip surface, the arrangement of the guide tip surface can better divide the fluid into two paths on one hand, and on the other hand, the guide tip surface can realize the necking effect, reduce the sectional area of the position and avoid the influence between the light sources; the ultraviolet-visible absorption and fluorescence spectrum can be measured simultaneously, and the phenomenon that signals of two light paths interfere with each other is avoided.
An ultraviolet-visible light incident hole 3 and an ultraviolet-visible light detection hole 5 are arranged on the tank body 7 perpendicular to the direction of the first liquid flow path, and the ultraviolet-visible light incident hole 3 and the ultraviolet-visible light detection hole 5 are oppositely arranged; a fluorescence spectrum light incident hole 4 and a fluorescence spectrum light detection hole 6 are arranged on the tank body 7 perpendicular to the direction of the second liquid flow path, and the fluorescence spectrum light incident hole 4 and the fluorescence spectrum light detection hole 6 are distributed in a vertical right angle; in this embodiment, the light source emitted from the ultraviolet-visible light incident hole 3 is a broadband light source, and the light source emitted from the fluorescence spectrum light incident hole 4 is a single wavelength laser light source.
In the specific implementation process, the parts except the ultraviolet visible light incident hole 3, the ultraviolet visible light detection hole 5, the fluorescence spectrum light incident hole 4 and the fluorescence spectrum light detection hole 6 in the cell body 7 are filled with light blocking materials, so that mutual interference of two light paths is prevented.
In the embodiment, a broadband light source is adopted in the ultraviolet-visible absorption spectrum, a single-wavelength laser light source is adopted in the fluorescence spectrum, and two paths of signal light enter a spectrum detection instrument, so that the simultaneous detection of the ultraviolet-visible absorption spectrum and the fluorescence spectrum can be realized; in addition, the intensity of the broadband light source adopted by ultraviolet-visible absorption is different from that of the laser light source adopted by fluorescence, the common detector may cause overexposure or excessively low signal and is difficult to switch, but the embodiment adopts a mode of separate detection, so that the phenomenon of overexposure or excessively low signal possibly occurring when the detector switches the measurement mode is avoided.
Example 2
The present embodiment provides a flow cell for simultaneously measuring ultraviolet-visible absorption and fluorescence spectrum, as shown in fig. 4, which has substantially the same structure, and the main difference is that: the liquid inlet 1 and the liquid outlet 2 on the cell body 7 are arranged on the same side of the cell body 7, one ends of the first liquid flow path and the second liquid flow path are communicated to form a closed state, the other ends of the first liquid flow path and the second liquid flow path are respectively communicated with the liquid inlet 1 and the liquid outlet 2, and more specifically, in the embodiment, the liquid flow paths form U-shaped structural communication from the liquid inlet 1 to the liquid outlet 2.
In the embodiment, a broadband light source is adopted in the ultraviolet-visible absorption spectrum, a single-wavelength laser light source is adopted in the fluorescence spectrum, and two paths of signal light enter a spectrum detection instrument to realize simultaneous detection of the ultraviolet-visible absorption spectrum and the fluorescence spectrum. Compared with the operation mode of the embodiment 1, the operation mode can change the components detected in sequence during the real-time detection of reactants, is mainly used for a liquid chromatograph, and is suitable for the detection and analysis of liquid.
While the utility model has been described with reference to an illustrative embodiment, it will be understood by those skilled in the art that various other changes, omissions and/or additions may be made and substantial equivalents may be substituted for elements thereof without departing from the spirit and scope of the utility model. In addition, many modifications may be made to adapt a particular situation or material to the teachings of the utility model without departing from the scope thereof. Therefore, it is intended that the utility model not be limited to the particular embodiment disclosed for carrying out this utility model, but that the utility model will include all embodiments falling within the scope of the appended claims. Moreover, unless specifically stated any use of the terms first, second, etc. do not denote any order or importance, but rather the terms first, second, etc. are used to distinguish one element from another.
Claims (10)
1. The flow cell for simultaneously measuring ultraviolet-visible absorption and fluorescence spectrum is characterized by comprising a cell body (7), wherein a liquid flow path is arranged in the cell body (7), and a liquid inlet (1) and a liquid outlet (2) which are communicated with the liquid flow path are also arranged on the cell body (7); the liquid flow paths comprise a first liquid flow path and a second liquid flow path, an ultraviolet visible light incident hole (3) and an ultraviolet visible light detection hole (5) are arranged on the tank body (7) perpendicular to the direction of the first liquid flow path, and the ultraviolet visible light incident hole (3) and the ultraviolet visible light detection hole (5) are oppositely arranged; the cell body (7) is provided with a fluorescence spectrum light incidence hole (4) and a fluorescence spectrum light detection hole (6) perpendicular to the direction of the second liquid flow path, and the fluorescence spectrum light incidence hole (4) and the fluorescence spectrum light detection hole (6) are distributed in a perpendicular right angle.
2. The flow cell for simultaneous measurement of uv-vis absorption and fluorescence spectra according to claim 1, wherein: the parts of the tank body (7) except the ultraviolet visible light incidence hole (3), the ultraviolet visible light detection hole (5), the fluorescence spectrum light incidence hole (4) and the fluorescence spectrum light detection hole (6) are filled with light blocking materials.
3. Flow cell for simultaneous measurement of uv-vis absorption and fluorescence spectra according to claim 1 or 2, characterized in that: the liquid inlet (1) and the liquid outlet (2) on the tank body (7) are respectively arranged on two mutually parallel side surfaces on the tank body (7), and two ends of the first liquid flow path and the second liquid flow path are respectively communicated with the liquid inlet (1) and the liquid outlet (2).
4. A flow cell for simultaneous measurement of uv-vis absorption and fluorescence spectra according to claim 3, wherein: the first liquid flow path and the second liquid flow path are symmetrically arranged along the connecting line of the liquid inlet (1) and the liquid outlet (2).
5. The flow cell for simultaneous measurement of uv-vis absorption and fluorescence spectra of claim 4, wherein: the liquid flow path is divided into two parts from the liquid inlet (1), and extends and converges to the liquid outlet (2) along the first liquid flow path and the second liquid flow path respectively.
6. The flow cell for simultaneous measurement of uv-vis absorption and fluorescence spectra of claim 5, wherein: the liquid inlet (1) is provided with a liquid inflow guide tip face, and the liquid outlet (2) is provided with a liquid outflow guide tip face.
7. Flow cell for simultaneous measurement of uv-vis absorption and fluorescence spectra according to claim 1 or 2, characterized in that: the liquid inlet (1) and the liquid outlet (2) on the tank body (7) are arranged on the same side face on the tank body (7), one ends of the first liquid flow path and the second liquid flow path are communicated to form a closed state, and the other ends of the first liquid flow path and the second liquid flow path are respectively communicated with the liquid inlet (1) and the liquid outlet (2).
8. The flow cell for simultaneous measurement of uv-vis absorption and fluorescence spectra of claim 7, wherein: the liquid flow path is communicated with the liquid outlet (2) from the liquid inlet (1) to form a U-shaped structure.
9. Flow cell for simultaneous measurement of uv-vis absorption and fluorescence spectra according to claim 1 or 2, characterized in that: the light source emitted by the ultraviolet visible light incident hole (3) adopts a broadband light source.
10. Flow cell for simultaneous measurement of uv-vis absorption and fluorescence spectra according to claim 1 or 2, characterized in that: the light source emitted by the fluorescence spectrum light incidence hole (4) adopts a single-wavelength laser light source.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322251290.4U CN220730039U (en) | 2023-08-21 | 2023-08-21 | Flow cell for simultaneously measuring ultraviolet-visible absorption and fluorescence spectrum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322251290.4U CN220730039U (en) | 2023-08-21 | 2023-08-21 | Flow cell for simultaneously measuring ultraviolet-visible absorption and fluorescence spectrum |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220730039U true CN220730039U (en) | 2024-04-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322251290.4U Active CN220730039U (en) | 2023-08-21 | 2023-08-21 | Flow cell for simultaneously measuring ultraviolet-visible absorption and fluorescence spectrum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220730039U (en) |
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2023
- 2023-08-21 CN CN202322251290.4U patent/CN220730039U/en active Active
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