CN220724170U

CN220724170U - Cell culture sampling device

Info

Publication number: CN220724170U
Application number: CN202321895213.6U
Authority: CN
Inventors: 王煜洲; 赵艳国
Original assignee: Lepu Beijing Biotechnology Co ltd
Current assignee: Lepu Beijing Biotechnology Co ltd
Priority date: 2023-07-18
Filing date: 2023-07-18
Publication date: 2024-04-05
Anticipated expiration: 2033-07-18

Abstract

The utility model discloses a cell culture sampling device, which comprises a culture tube, wherein a sampling mechanism is arranged on the outer side of the culture tube, the sampling mechanism comprises a fixing frame fixedly connected to the outer side of the culture tube, one end of the fixing frame is fixedly connected with a clamping groove, the inner side of the clamping groove is movably connected with a sampling tube, the upper end of the sampling tube is fixedly connected with a sampling hose, the outer side of the sampling tube is fixedly connected with a mounting frame near the lower side of the clamping groove, and the inner side of the mounting frame is in threaded connection with a sampling head near the lower side of the sampling tube. According to the cell culture sampling device, the cultured cells are sucked into the inner side of the sampling tube through the squeezed sampling hose, the sampling hose is restored to the original state, the limiting ball moves downwards under the action of gravity and the limiting ball blocks the limiting ring, so that the purpose that the limiting ball limits a sampling object in the inner side of the sampling tube is achieved, and the condition that the cultured cells flow out of the sampling head is avoided.

Description

Cell culture sampling device

Technical Field

The utility model relates to the technical field of cell culture, in particular to a cell culture sampling device.

Background

Cell culture refers to a method of simulating in vitro an in vivo environment (sterile, suitable temperature, ph, certain nutritional conditions, etc.) to survive, grow, reproduce and maintain major structures and functions. Cell culture is also known under the generic term cell culture technology in biology. Cell culture is an essential process for either whole or one of them, which is itself a large scale of cells;

the conventional cell culture sampling device has some disadvantages, and when the cell culture sampling device is used, since most of the sampling devices are not provided with an automatic closing structure, after the sampling device takes out the sampled objects, the sampled objects flow backwards along the sampling device, so that part of the sampled objects flow out, and the flowing out sampled objects are polluted.

Disclosure of Invention

The utility model mainly aims to provide a cell culture sampling device which can effectively solve the problems in the background technology.

In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows:

the utility model provides a cell culture sampling device, includes the cultivation pipe, the outside of cultivateing the pipe is provided with sampling mechanism, sampling mechanism includes the mount of fixed connection in the cultivation pipe outside, the one end fixedly connected with draw-in groove of mount, the inboard swing joint of draw-in groove has the sampling tube, the upper end fixedly connected with sampling hose of sampling tube, the downside fixedly connected with mounting bracket that the outside of sampling tube is close to the draw-in groove, the inboard of mounting bracket is close to the downside threaded connection of sampling tube has the sampling head, the inboard inner wall fixedly connected with restriction ring of sampling head, the upper end swing joint of restriction ring has the restriction ball, the inboard inner wall of sampling head is close to the upper end fixedly connected with restriction frame of restriction ring.

Preferably, a limiting ring is fixedly connected between the outer side of the sampling tube, which is close to the clamping groove, and the mounting frame, and the upper end of the limiting ring is movably connected with the lower end of the clamping groove.

Preferably, the main body of the sampling hose is made of rubber, and the axis lines of the sampling tube, the mounting frame and the sampling head are positioned on the same straight line.

Preferably, the sampling head is connected to the lower end of the sampling tube through a mounting frame, and the lower end of the sampling head is in a slope shape.

Preferably, the axes of the sampling head, the limiting ring, the limiting ball and the limiting frame are positioned on the same straight line, the number of the limiting rings is two, and the two limiting rings are distributed in a central symmetry mode.

Preferably, the upper end of the culture tube is fixedly connected with an external thread head, the outer side of the external thread head is in threaded connection with an internal thread cover, and the axial leads of the culture tube, the external thread head and the internal thread cover are positioned on the same straight line.

Compared with the prior art, the utility model has the following beneficial effects:

1. the purpose that the limiting ball limits the sampling object in the inner side of the sampling tube can be achieved, so that the condition that the cultured cells flow out of the sampling head is avoided.

2. Secondly, the sampling head is rotated, and the sampling head is separated from the mounting frame, so that the purposes of conveniently cleaning the sampling tube and the sampling head can be achieved.

Drawings

FIG. 1 is a schematic diagram showing the overall structure of a cell culture sampling device according to the present utility model;

FIG. 2 is a schematic diagram showing a partial structure of a cell culture sampling device according to the present utility model;

FIG. 3 is a schematic diagram of a sampling mechanism of a cell culture sampling device according to the present utility model;

FIG. 4 is a schematic diagram showing a partial structure of a sampling mechanism of a cell culture sampling device according to the present utility model.

In the figure: 1. a culture tube; 2. an external thread head; 3. an internal thread cap; 4. a sampling mechanism; 41. a fixing frame; 42. a clamping groove; 43. a sampling tube; 44. a sampling hose; 45. a mounting frame; 46. a sampling head; 47. a confinement ring; 48. a limit ball; 49. a limiting frame; 410. and a limiting ring.

Detailed Description

The utility model is further described in connection with the following detailed description, in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the utility model easy to understand.

As shown in fig. 1-4, a cell culture sampling device comprises a culture tube 1, a sampling mechanism 4 is arranged on the outer side of the culture tube 1, the sampling mechanism 4 comprises a fixing frame 41 fixedly connected to the outer side of the culture tube 1, one end of the fixing frame 41 is fixedly connected with a clamping groove 42, a sampling tube 43 is movably connected to the inner side of the clamping groove 42, the upper end of the sampling tube 43 is fixedly connected with a sampling hose 44, the outer side of the sampling tube 43 is close to a mounting frame 45 fixedly connected to the lower side of the clamping groove 42, the inner side of the mounting frame 45 is close to a sampling head 46 in threaded connection with the lower side of the sampling tube 43, a limiting ring 47 is fixedly connected to the inner side inner wall of the sampling head 46, a limiting ball 48 is movably connected to the upper end of the limiting ring 47, and the inner side inner wall of the sampling head 46 is close to a limiting frame 49.

In this embodiment, a limiting ring 410 is fixedly connected between the outer side of the sampling tube 43 and the clamping groove 42 and the mounting frame 45, the upper end of the limiting ring 410 is movably connected with the lower end of the clamping groove 42, the main body of the sampling hose 44 is made of rubber, the sampling tube 43, the mounting frame 45 and the axial leads of the sampling head 46 are positioned on the same straight line, the sampling head 46 is connected with the lower end of the sampling tube 43 through the mounting frame 45, the lower end of the sampling head 46 is in a slope shape, the axial leads of the sampling head 46, the limiting ring 47, the limiting ball 48 and the limiting frame 49 are positioned on the same straight line, two groups of limiting rings 410 are arranged, and the two groups of limiting rings 410 are distributed in a central symmetry manner.

Specifically, squeezing the sampling hose 44, screwing the sampling head 46 into the inside of the mounting frame 45, inserting the sampling head 46 into the inside of the culture tube 1, and then loosening the sampling hose 44, thereby sucking up the sampling hose 44 from the inside of the sampling tube 43, and moving the restriction ball 48 upward, and bringing the restriction ball 48 into contact with the restriction frame 49, while the cells cultured in the inside of the culture tube 1 are sucked into the inside of the sampling head 46, and flowing the cells into the inside of the sampling tube 43, and when the resampling hose 44 is restored to the original state, thereby moving the restriction ball 48 downward by gravity, and causing the restriction ball 48 to block the restriction ring 47, so that the cultured cells are prevented from flowing out of the sampling head 46, sucking the cultured cells into the inside of the sampling tube 43 through the squeezed sampling hose 44, and restoring the sampling hose 44 to the original state, while the restriction ball 48 moves downward by gravity, and causing the restriction ball 48 to block the restriction ring 47, the purpose of restricting the cells in the inside of the sampling tube 43 can be achieved, thereby avoiding the cultured cells flowing out of the sampling head 46.

In this embodiment, the upper end of the culture tube 1 is fixedly connected with an external thread head 2, the external thread of the external thread head 2 is connected with an internal thread cover 3, and the axes of the culture tube 1, the external thread head 2 and the internal thread cover 3 are positioned on the same straight line.

Specifically, by rotating the female cap 3 and disengaging the female cap 3 from the male head 2, it is possible to facilitate removal of the cells in the inside of the culture tube 1.

Working principle:

in use, firstly, cell culture is carried out in the inner side of the culture tube 1, and when sampling is required, the sampling tube 43 is pulled out firstly, and the sampling tube 43 is separated from the inner side of the clamping groove 42, meanwhile, the inner thread cover 3 is rotated again, and the inner thread cover 3 is separated from the outer thread head 2, then the sampling tube 44 is extruded, the sampling head 46 is screwed into the inner side of the mounting frame 45, and the sampling head 46 is inserted into the inner side of the culture tube 1 through the outer thread head 2, then the sampling tube 44 is loosened, so that the sampling tube 44 is sucked up from the inner side of the sampling tube 43, and the restriction ball 48 is moved up, and the restriction ball 48 is contacted with the restriction frame 49, meanwhile, the cultured cells in the inner side of the culture tube 1 are sucked into the inner side of the sampling head 46, and the cells are flowed into the inner side of the sampling tube 43, and the cells are stopped by the restriction ball 48 are stopped by the restriction frame 46, and the restriction ball 48 is restored to the inner side of the sampling tube 43, so that the restriction ball 46 is moved down under the action of gravity, and the restriction ball 46 is stopped by the restriction ring 47, so that the cultured cells flow out of the sampling tube 46 is prevented from flowing out of the sampling head 46, and the sampling tube 46 is sucked down by the restriction frame 46, and the restriction ball 46 is stopped by the restriction ring 45, and the inner side of the restriction frame 46 is stopped by the restriction of the inner side of the restriction ring 43, and the restriction ball 46 is sucked down, the sampling head 46 is cleaned for purposes of cleaning.

The foregoing has shown and described the basic principles and main features of the present utility model and the advantages of the present utility model. It will be understood by those skilled in the art that the present utility model is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present utility model, and various changes and modifications may be made without departing from the spirit and scope of the utility model, which is defined in the appended claims. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims

1. Cell culture sampling device, including cultivateing pipe (1), its characterized in that: the outside of cultivateing pipe (1) is provided with sampling mechanism (4), sampling mechanism (4) are including fixed connection mount (41) in cultivateing pipe (1) outside, the one end fixedly connected with draw-in groove (42) of mount (41), the inboard swing joint of draw-in groove (42) has sampling tube (43), the upper end fixedly connected with sampling hose (44) of sampling tube (43), the downside fixedly connected with mounting bracket (45) that the outside of sampling tube (43) is close to draw-in groove (42), the downside threaded connection that the inboard of mounting bracket (45) is close to sampling tube (43) has sampling head (46), the inboard inner wall fixedly connected with restriction ring (47) of sampling head (46), the upper end swing joint of restriction ring (47) restriction ball (48), the inboard inner wall of sampling head (46) is close to the upper end fixedly connected with restriction frame (49) of restriction ring (47).

2. The cell culture sampling apparatus of claim 1, wherein: a limiting ring (410) is fixedly connected between the outer side of the sampling tube (43) close to the clamping groove (42) and the mounting frame (45), and the upper end of the limiting ring (410) is movably connected with the lower end of the clamping groove (42).

3. A cell culture sampling apparatus according to claim 2, wherein: the main body of the sampling hose (44) is made of rubber, and the axial leads of the sampling tube (43), the mounting frame (45) and the sampling head (46) are positioned on the same straight line.

4. A cell culture sampling apparatus according to claim 2, wherein: the sampling head (46) is connected to the lower end of the sampling tube (43) through the mounting frame (45), and the lower end of the sampling head (46) is in a slope shape.

5. A cell culture sampling apparatus according to claim 2, wherein: the axial lines of the sampling head (46), the limiting rings (47), the limiting balls (48) and the limiting frames (49) are positioned on the same straight line, two groups of limiting rings (410) are arranged, and the two groups of limiting rings (410) are distributed in a central symmetry mode.

6. The cell culture sampling apparatus of claim 1, wherein: the upper end fixedly connected with external screw thread head (2) of cultivate pipe (1), the outside threaded connection of external screw thread head (2) has internal screw thread lid (3), cultivate the axial lead of pipe (1), external screw thread head (2), internal screw thread lid (3) is located same straight line.