CN220703672U

CN220703672U - Filtering structure and supernatant extraction device

Info

Publication number: CN220703672U
Application number: CN202321910379.0U
Authority: CN
Inventors: 杜智欣; 伦才智; 陈展册; 钟勇
Original assignee: Nanning Customs Technology Center
Current assignee: Nanning Customs Technology Center
Priority date: 2023-07-20
Filing date: 2023-07-20
Publication date: 2024-04-02
Anticipated expiration: 2033-07-20

Abstract

The utility model discloses a filtering structure and a supernatant extraction device, the filtering structure is applied to a centrifuge tube, the filtering structure comprises a filter screen, the filter screen is provided with a filter cavity and a plurality of filter holes communicated with the filter cavity, a support rod comprises a connecting section and an operating section which are connected, and the connecting section part extends into the filter cavity and is detachably connected with the bottom wall of the filter cavity. Adopt the filtration of this application, not only can improve the extraction efficiency of supernatant, simultaneously through linkage segment and the filter screen that can dismantle the connection, can avoid the lysate to flow between a plurality of containers, avoided the supernatant that draws to receive the circumstances of pollution to a great extent.

Description

Filtering structure and supernatant extraction device

Technical Field

The utility model relates to the technical field of specimen equipment, in particular to a filtering structure and a supernatant extraction device.

Background

Currently, nucleic acid extraction methods generally involve two major steps, cleavage and purification of a sample. The cleavage step refers to a process of dissociating nucleic acid in a cleavage system in a crushed sample, namely, a step of adding a proper amount of a cleavage solution into the crushed sample and incubating, wherein DNA is dissociated in the cleavage solution after the cleavage is finished, precipitation is required to be removed by centrifugation, and a supernatant is taken for the next purification step. However, some animal and plant materials such as rice flour, lotus root starch, bird's nest, fish maw, sea cucumber and the like, because of the characteristics of the materials, swelling phenomenon can occur when the cracking liquid is added after crushing, pasty, gelatinous and flocculent semi-solid substances are formed, the supernatant liquid is little or even no after centrifugation, the improvement is little after the centrifugal force is increased, and the requirements of subsequent purification are met by sampling, cracking and enrichment for multiple times, namely, the extraction efficiency of the supernatant liquid is lower.

In the related art, the supernatant is extracted by adopting a filtering mode, the lysate is required to be poured into another container, and then the pipetting gun is used for sucking the supernatant, so that the method is more troublesome, and the probability of pollution of the lysate is increased when the lysate is frequently moved, thereby influencing the experimental effect.

Disclosure of Invention

The utility model mainly aims to provide a supernatant extraction device, which aims to improve the extraction efficiency of supernatant and reduce the pollution probability of the supernatant.

In order to achieve the above object, the present utility model provides a filtering structure, applied to a centrifuge tube, comprising:

the filter screen is provided with a filter cavity and a plurality of filter holes communicated with the filter cavity; and

the support rod comprises a connecting section and an operating section which are connected, and the connecting section part extends into the filter cavity and is detachably connected with the bottom wall of the filter cavity;

the operation section drives the filter screen to extend into the centrifuge tube so that the supernatant flows into the filter cavity from the filter hole.

In an alternative embodiment, the filter screen is provided with a first magnetic member, the connecting section is provided with a second magnetic member, and the first magnetic member and the second magnetic member are magnetically attracted to connect the filter screen and the connecting section.

In an optional embodiment, the connecting section is concavely provided with a mounting slot towards one side of the filter screen, the second magnetic piece is embedded in the mounting slot, and the first magnetic piece and the filter screen are integrally formed.

In an optional embodiment, a positioning notch is formed on one side, facing the filter screen, of the second magnetic member, and a positioning protrusion formed by protruding on the bottom wall of the filter cavity is embedded in the positioning notch.

In an optional embodiment, the filtering structure further comprises a power-assisted sleeve, the power-assisted sleeve is sleeved on the outer side of the supporting rod, the connecting section is connected with the filter screen, and the end part of the power-assisted sleeve is attached to the bottom wall of the filter cavity.

In an alternative embodiment, the end of the power sleeve remote from the filter screen is provided with a power flange.

In an alternative embodiment, the filter screen comprises a connected screen body and a sealing flange, wherein the screen body is provided with the filter cavity and a plurality of filter holes, and the sealing flange is annularly arranged at the opening end of the filter cavity;

the net body is accommodated in the centrifugal tube, and the sealing flange and part of the inner wall surface of the centrifugal tube enclose to form an extrusion cavity.

In an optional embodiment, the filter screen comprises a support grid, a sealing flange and filter cloth, wherein the filter cloth is connected to the support grid and is enclosed with the support grid to form a filter cavity, and a plurality of filter holes are formed in the filter cloth;

the support grid is contained in the centrifugal tube, and the sealing flange, the filter cloth and part of the inner wall surface of the centrifugal tube enclose to form an extrusion cavity.

The utility model also provides a supernatant extraction device which comprises the filtering structure.

In an alternative embodiment, the supernatant extraction device comprises a centrifuge tube and the filtration structure comprises:

The filter structure provided by the utility model is applied to a centrifuge tube, and comprises a filter screen, wherein the filter screen is provided with a filter cavity and a plurality of filter holes communicated with the filter cavity, and the support rod comprises a connecting section and an operating section which are connected, and part of the connecting section extends into the filter cavity and is detachably connected with the bottom wall of the filter cavity. In this embodiment, when the supernatant liquid needs to be extracted, through the operation section of operation bracing piece, drive linkage segment and filter screen stretch into the centrifuging tube, through the filtration effect of a plurality of filtration holes on the filter screen, can make the supernatant liquid flow into the filter chamber in, and isolate flocculent semi-solid substance in the centrifuging tube, after the supernatant liquid flows into the filter chamber in, demolish linkage segment and filter screen, adopt the liquid-transfering gun to draw the supernatant liquid in the filter chamber. So adopt the filtration of this application, not only can improve the extraction efficiency of supernatant, through linkage segment and the filter screen that can dismantle the connection simultaneously, can avoid the lysate to flow between a plurality of containers, avoided the supernatant that draws to receive the circumstances of pollution to a great extent.

Drawings

In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are only some embodiments of the present utility model, and other drawings may be obtained according to the structures shown in these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic view of an embodiment of a filter structure according to the present utility model;

FIG. 2 is a schematic diagram showing the structure of an embodiment of the supernatant extraction apparatus of the present utility model;

FIG. 3 is an exploded view of the supernatant extraction device shown in FIG. 2;

FIG. 4 is a top view of the supernatant extraction device shown in FIG. 2;

FIG. 5 is a cross-sectional view of the supernatant extraction device shown in FIG. 4 taken along the direction A-A;

fig. 6 is an enlarged detail view at a in fig. 5.

Reference numerals illustrate:

the achievement of the objects, functional features and advantages of the present utility model will be further described with reference to the accompanying drawings, in conjunction with the embodiments.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It should be noted that all directional indicators (such as up, down, left, right, front, and rear … …) in the embodiments of the present utility model are merely used to explain the relative positional relationship, movement, etc. between the components in a particular posture (as shown in the drawings), and if the particular posture is changed, the directional indicator is changed accordingly.

Furthermore, the description of "first," "second," etc. in this disclosure is for descriptive purposes only and is not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In addition, the technical solutions of the embodiments may be combined with each other, but it is necessary to base that the technical solutions can be realized by those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent and not within the scope of protection claimed in the present utility model.

In an embodiment of the present utility model, the filter structure 10 is applied to a centrifuge tube 20, and the filter structure 10 includes: a filter screen 11, wherein the filter screen 11 is provided with a filter cavity 11a and a plurality of filter holes 11b communicated with the filter cavity 11 a; a support rod 12, wherein the support rod 12 comprises a connecting section 121 and an operating section 122 which are connected, and the connecting section 121 partially extends into the filter cavity 11a and is detachably connected with the cavity bottom wall of the filter cavity 11 a; the operation section 122 drives the filter screen 11 to extend into the centrifuge tube 20, so that the supernatant flows into the filter cavity 11a from the filter hole 11 b.

In this embodiment, the centrifuge tube 20 is a commercially available common centrifuge tube 20, and the volume of the centrifuge tube may be 1.5ml or 2.0ml, and may be selected according to actual needs. The filter screen 11 may be made of medical grade plastic material or metal material, and is tapered as a whole. The connecting section 121 and the operating section 122 of the supporting rod 12 are integrally formed, the connecting section 121 is used for connecting the filter screen 11, and the operating section 122 is used for facilitating operation of a user.

In this embodiment, when the supernatant is required to be extracted, the operation section 122 of the support rod 12 is operated to drive the connection section 121 and the filter screen 11 to extend into the centrifuge tube 20, the supernatant can flow into the filter cavity 11a through the filtering action of the plurality of filter holes 11b on the filter screen 11, the flocculent semi-solid substance is isolated in the centrifuge tube 20, and after the supernatant flows into the filter cavity 11a, the connection section 121 and the filter screen 11 are removed, and the supernatant in the filter cavity 11a is extracted by using a pipette. By adopting the filtering structure 10 of the application, not only the extraction efficiency of the supernatant liquid can be improved, but also the flowing of the pyrolysis liquid among a plurality of containers can be avoided through the connecting section 121 and the filter screen 11 which are detachably connected, the condition that the extracted supernatant liquid is polluted can be avoided to a great extent,

in one embodiment, the filter 11 is provided with a first magnetic member (not shown), the connecting section 121 is provided with a second magnetic member 123, and the first magnetic member and the second magnetic member 123 are magnetically attracted to connect the filter 11 and the connecting section 121.

It is understood that one of the first magnetic member and the second magnetic member 123 may be a magnet, and the other one of the two may be a metal material such as steel, iron, or the like, which may be magnetically attracted by the magnet. Or both the first magnetic member and the second magnetic member 123 may be magnets. The filter screen 11 and the connecting section 121 are magnetically attracted, so that the filter screen and the connecting section are convenient to assemble and disassemble.

In an embodiment, the connecting section 121 faces to one side of the filter screen 11, that is, one side of the connecting section 121 facing away from the operating section 122, an installation clamping groove 121a is formed by machining, the second magnetic piece 123 is embedded in the installation clamping groove 121a, the second magnetic piece 123 is a magnet, the first magnetic piece and the filter screen 11 are integrally formed, that is, the first magnetic piece and the filter screen 11 are integrally formed, or the filter screen 11 is made of plastic material, the first magnetic piece is a magnet, and the first magnetic piece and the filter screen 11 are integrally formed through an in-mold forming process. In this application, the mounting structure through second magnetic part 123 and first magnetic part is simple, and the manufacturing of just facilitating the production.

Referring to fig. 6 again, further, a positioning notch 123a is disposed on a side of the second magnetic member 123 facing the filter screen 11, and a positioning protrusion 111 is formed on a bottom wall of the filter cavity 11a, and when the second magnetic member 123 magnetically attracts the filter screen 11, the positioning protrusion 111 can be embedded in the positioning notch 123 a. In this application, the switch of location arch 111 is circular, and the axle center coincides with the axle center of filtering the wind, through setting up this location arch 111 for linkage segment 121 can accurately coincide with filter screen 11, and filter screen 11 stretches into to centrifuging tube 20 in, and when filtering the pyrolysis liquid, the atress is comparatively balanced.

Still further, the filtering structure 10 further includes a power-assisted sleeve 13, the diameter of the power-assisted sleeve 13 is slightly larger than the diameter of the supporting rod 12, that is, the power-assisted sleeve 13 is in clearance fit with the supporting rod 12, the power-assisted sleeve 13 is sleeved on the outer side of the supporting rod 12, the connecting section 121 is magnetically connected to the filtering screen 11 through the first magnetic member and the second magnetic member 123, and the end of the power-assisted sleeve 13 is attached to the bottom wall of the filtering cavity 11 a. In actual use, when needs demolish linkage segment 121 and filter screen 11, the user exerts the ascending power of direction to operation section 122, and helping hand sleeve pipe 13 exerts decurrent power simultaneously, so the user can stabilize filter screen 11 in centrifuging tube 20 through helping hand sleeve pipe 13, and linkage segment 121 and filter screen 11's dismantlement is comparatively convenient simultaneously.

Further, the end portion of the power-assisted sleeve 13 far away from the filter screen 11 is provided with a power-assisted flange 131, and the power-assisted flange 131 and the filter screen 11 are of an integrated structure, so that a user can conveniently apply downward acting force to the power-assisted sleeve 13 through the power-assisted flange 131.

In an embodiment of the present utility model, the filter screen 11 includes a screen body 112 and a sealing flange 113 that are connected, where the screen body 112 and the sealing flange 113 are integrally formed, the screen body 112 is conical, the sealing flange 113 is disposed around an opening end of the filter cavity 11a, in practical application, the screen body 112 is accommodated in the centrifuge tube 20, and the sealing flange 113 and an inner wall surface of a portion of the centrifuge tube 20 enclose to form an extrusion cavity 113a. In this application, through setting up this sealing flange 113, can avoid the cell body 112 when filtering the lysate, the open end of lysate filter chamber 11a overflows into filter chamber 11a to improve the filter effect of supernatant.

In another embodiment of the present utility model, the filter screen 11 includes a support grid, a sealing flange 113 and a filter cloth, wherein the filter cloth is adhered to the support grid by glue and enclosed with the support grid to form a filter cavity 11a, a plurality of filter holes 11b are formed in the filter cloth, the sealing flange 113 is connected with the support grid, and the sealing flange 113, the filter cloth and part of the inner wall surface of the centrifuge tube 20 enclose to form an extrusion cavity 113a. That is, the filter screen 11 of the present application is in a split arrangement, and a user can replace filter cloth with different sizes according to actual needs, so that the filter screen can better adapt to the needs of different substance pyrolysis solutions, and the compatibility of the filter structure 10 is better.

The utility model also provides a supernatant extraction device 100, wherein the supernatant extraction device 100 comprises a centrifuge tube 20 and a filtering structure 10, and the specific structure of the filtering structure 10 refers to the above embodiment, and since the supernatant extraction device 100 adopts all the technical solutions of all the embodiments, at least has all the beneficial effects brought by the technical solutions of the embodiments, and will not be described in detail herein. Wherein the filter structure 10 comprises: a filter screen 11, wherein the filter screen 11 is provided with a filter cavity 11a and a plurality of filter holes 11b communicated with the filter cavity 11 a; the support rod 12 comprises a connecting section 121 and an operating section 122 which are connected, wherein the connecting section 121 partially extends into the filter cavity 11a and is detachably connected with the cavity bottom wall of the filter cavity 11 a; the operation section 122 drives the filter screen 11 to extend into the centrifuge tube 20 so that the supernatant flows into the filter chamber 11a through the filter holes 11 b.

The foregoing description is only of the preferred embodiments of the present utility model and is not intended to limit the scope of the utility model, and all equivalent structural changes made by the description of the present utility model and the accompanying drawings or direct/indirect application in other related technical fields are included in the scope of the utility model.

Claims

1. A filtration structure applied to a centrifuge tube, the filtration structure comprising:

2. The filter structure of claim 1, wherein the filter screen is provided with a first magnetic member, the connecting section is provided with a second magnetic member, and the first magnetic member and the second magnetic member are magnetically attracted to connect the filter screen and the connecting section.

3. The filter structure of claim 2, wherein the connecting section is concavely provided with a mounting slot toward one side of the filter screen, the second magnetic member is embedded in the mounting slot, and the first magnetic member and the filter screen are integrally formed.

4. A filter structure according to claim 3, wherein a positioning notch is formed on a side of the second magnetic member facing the filter screen, and a positioning protrusion formed on a bottom wall of the filter cavity is protruding, and the positioning protrusion can be embedded in the positioning notch.

5. The filter structure of any one of claims 1 to 4, further comprising a booster sleeve, the booster sleeve being sleeved outside the support rod, the connection section being connected to the filter screen, an end of the booster sleeve being attached to a bottom wall of the filter cavity.

6. The filter structure of claim 5, wherein the end of the boost sleeve remote from the filter screen is provided with a boost flange.

7. The filter structure according to any one of claims 1 to 4, wherein the filter screen comprises a connected screen body provided with the filter chamber and a plurality of the filter holes and a sealing flange looped over an open end of the filter chamber;

8. The filter structure according to any one of claims 1 to 4, wherein the filter screen includes a support grid, a sealing flange, and a filter cloth connected to the support grid and enclosing with the support grid to form a filter cavity, and a plurality of filter holes are provided in the filter cloth;

9. A supernatant extraction device comprising a filter structure according to any one of claims 1 to 8.

10. The supernatant extraction device of claim 9, wherein the supernatant extraction device comprises a centrifuge tube and the filtration structure, the filtration structure comprising: