CN220665296U - A required constant temperature water bath for producing CA199 protein's cell is resuscitated - Google Patents
A required constant temperature water bath for producing CA199 protein's cell is resuscitated Download PDFInfo
- Publication number
- CN220665296U CN220665296U CN202322264099.3U CN202322264099U CN220665296U CN 220665296 U CN220665296 U CN 220665296U CN 202322264099 U CN202322264099 U CN 202322264099U CN 220665296 U CN220665296 U CN 220665296U
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- China
- Prior art keywords
- water bath
- producing
- objective table
- lead screw
- bin
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 63
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 16
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 16
- 239000011521 glass Substances 0.000 claims abstract description 8
- 238000007789 sealing Methods 0.000 claims abstract description 7
- 238000005192 partition Methods 0.000 claims description 12
- 238000005138 cryopreservation Methods 0.000 claims description 3
- 230000014616 translation Effects 0.000 claims 1
- 238000007710 freezing Methods 0.000 abstract description 14
- 230000008014 freezing Effects 0.000 abstract description 14
- 241000894006 Bacteria Species 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 3
- 238000011109 contamination Methods 0.000 abstract description 2
- 238000011084 recovery Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000739 chaotic effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a constant-temperature water bath kettle which is used for producing CA199 protein and is required for cell resuscitation, wherein a sealing bottom bin and a water bin are arranged on a constant-temperature water bath kettle body, a lead screw is rotationally arranged on the constant-temperature water bath kettle body, one end of the lead screw is arranged in the water bin and is in threaded connection with an objective table, the other end of the lead screw extends into the sealing bottom bin and is fixedly connected with a rotating shaft of a driving motor, the objective table is in sliding connection with the water bin, and a plurality of clamping holes for inserting a freezing tube are formed in the objective table. The utility model can recover a plurality of CA199 cells at one time, reduces manpower and material resources, saves time, has high efficiency, and reduces the risk of bacteria contamination by fixing the freezing tube in the fixing ring. The transparent glass cover is arranged at the top of the water bath kettle, so that the recovery condition of internal cells can be observed at any time, the freezing storage tube can be prevented from being burst, and workers can be splashed.
Description
Technical Field
The utility model relates to a constant-temperature water bath kettle for cell resuscitation for producing CA199 protein.
Background
At present, the traditional cell resuscitating mode mainly utilizes a thermostatic water bath to set to 37 ℃, an operator squeezes a cell freezing tube by bare hands or with tools such as tweezers, part of the cell freezing tube is immersed into the thermostatic water bath, in the actual production process, when a plurality of cells secreting CA199 protein are resuscitated, if only one person operates, the process is slow, the working efficiency is very low, if a plurality of persons operate, the area of the water bath is limited, the table surface is chaotic, the resuscitating effect is influenced, the bacteria infection risk is increased, the time and material resources are consumed, the resuscitating effect of each cell strain is inconsistent, and the burst risk exists due to uneven quality of the freezing tube.
Disclosure of Invention
The utility model aims to solve the problems in the prior art and provides a constant-temperature water bath kettle required for cell resuscitation for producing CA199 protein.
The utility model adopts the technical scheme that:
the utility model provides a required constant temperature water bath during cell resuscitation for producing CA199 protein, includes constant temperature water bath body, objective table, lead screw and driving motor, is equipped with sealed end bin and sump on the constant temperature water bath body, and the lead screw rotates to locate on the constant temperature water bath body, and one end place in the sump and with objective table threaded connection, the other end stretch in sealed end bin and with driving motor's pivot fixed connection, objective table sliding connection is equipped with a plurality of card holes that are used for pegging graft the cryopreservation pipe on the objective table in the sump.
Further, a partition plate is arranged on the constant-temperature water bath body, the partition plate divides the inner cavity of the constant-temperature water bath body into two parts, the sealed bottom bin and the water bin are correspondingly formed, the screw rod is rotationally connected to the partition plate, and a sealing ring is arranged between the screw rod and the partition plate.
Further, the objective table is of a disc structure, an upward bulge is arranged at the circle center of the objective table, the lead screw is in threaded connection with the bulge, a sliding block is arranged on the outer wall of the objective table, a sliding groove is arranged on the inner wall of the water sump, and the sliding block is in sliding connection with the sliding groove.
Further, a connecting pipe is arranged in the inner cavity of the bulge, and the lead screw is in threaded connection with the connecting pipe.
Further, the lead screw comprises a polished rod part and a screw rod part, wherein the polished rod part is rotationally connected to the partition plate through a bearing, and the screw rod part is in threaded connection with the connecting pipe.
Further, a fixed support is arranged in the sealed bottom bin, and the screw rod is rotationally connected to the fixed support.
Further, a rubber fixing ring is arranged in the clamping hole.
Further, a transparent glass cover is covered at the top end of the water bin.
Further, a window for observing the water level is arranged on the side wall of the water storage bin of the water bath.
The utility model has the following beneficial effects:
the utility model can recover a plurality of CA199 cells at one time, reduces manpower and material resources, saves time, has high efficiency, and reduces the risk of bacteria contamination by fixing the freezing tube in the fixing ring. The transparent glass cover is arranged at the top of the water bath kettle, so that the recovery condition of internal cells can be observed at any time, the freezing storage tube can be prevented from being burst, and workers can be splashed.
Drawings
Fig. 1 is a structural diagram of the present utility model.
FIG. 2 is a schematic view of a rubber retaining ring disposed within a snap hole.
Detailed Description
The utility model is further described below with reference to the accompanying drawings.
Referring to fig. 1 and 2, the utility model relates to a thermostat water bath required during cell resuscitation for producing CA199 protein, which comprises a thermostat water bath body, an objective table 3, a lead screw 9 and a driving motor 8, wherein a sealing bottom bin 5 and a water bin 6 are arranged on the thermostat water bath body, the lead screw 9 is rotationally arranged on the thermostat water bath body, the upper end of the lead screw 9 is arranged in the water bin 6 and is in threaded connection with the objective table 3, the lower end of the lead screw is extended into the sealing bottom bin 5 and is fixedly connected with a rotating shaft of the driving motor 8, the objective table 3 is slidably connected in the water bin 6, a plurality of clamping holes 4 for inserting a freezing tube are arranged on the objective table 3, and in order to ensure the inserting stability of the freezing tube in the clamping holes 4, a rubber fixing ring 41 is fixed in the clamping holes 4, and interference fit between the freezing tube and the clamping holes 4 is realized through the arrangement of the rubber fixing ring 41.
The sealed bottom bin 5 and the water bin 6 in the constant-temperature water bath kettle body are formed by arranging a partition plate 13 in the inner cavity of the constant-temperature water bath kettle body, the inner cavity of the constant-temperature water bath kettle body is divided into two parts by the partition plate 13, the sealed bottom bin 5 and the water bin 6 are formed, the screw rod 9 is rotationally connected to the partition plate 13, and a sealing ring is arranged between the screw rod 9 and the partition plate 13 in order to prevent liquid in the water bin 6 from leaking into the sealed bottom bin 5.
The object stage 3 is of a disc structure, an upward bulge 31 is arranged at the center of the object stage 3, a connecting pipe 32 is arranged in the inner cavity of the bulge 31, and the lead screw 9 is in threaded connection with the connecting pipe 32.
The sliding assembly structure between the objective table 3 and the water sump 6 is as follows: the outer wall of the object stage 3 is provided with a sliding block, the inner wall of the water sump 6 is provided with a sliding groove 61, and the sliding block is in sliding connection with the sliding groove 61.
In order to simultaneously realize the rotational assembly between the screw rod 9 and the partition plate 13 and the threaded connection between the screw rod 9 and the object stage 3, the outer wall surface of the screw rod 9 is set to be two sections, namely, one is a polished rod part with a smooth surface structure, and the other is a threaded rod part with a threaded surface structure, the polished rod part is rotationally connected to the partition plate 13 through a bearing, and the threaded rod part is in threaded connection with the connecting pipe 32.
In order to ensure the rotation stability of the screw rod 9, a fixed bracket 11 is arranged in the sealed bottom bin 5, and the screw rod 9 is rotationally connected to the fixed bracket 11.
The top end of the water bin 6 is covered with a transparent glass cover 2, the transparent glass cover 2 can prevent liquid in the water bin 6 from splashing, a handle is arranged on the transparent glass cover 2, and a window for observing the water level is arranged on the side wall of the water storage bin 6 of the water bath.
The utility model has simple structure and convenient operation, firstly, water with the temperature of 37 ℃ is added into the water storage bin to reach a required water level line, then the freezing storage pipes of cells to be resuscitated are inserted into the clamping holes 4, the top ends of the freezing storage pipes are sealed, then the switch on the driving motor 8 is turned on, and the up-and-down movement of the freezing storage pipes in the water with the temperature of 37 ℃ is realized through the periodical forward and backward rotation of the driving motor 8. And observing the cell resuscitating condition through the transparent glass cover 2, when the object stage 3 is driven by the observation driving motor 8 to approximately rise to the vertex position after resuscitating is completed, closing the driving motor 8, and finally taking out the resuscitated cells of the cryopreservation tube.
The foregoing is merely a preferred embodiment of the utility model, and it should be noted that modifications could be made by those skilled in the art without departing from the principles of the utility model, which modifications would also be considered to be within the scope of the utility model.
Claims (9)
1. A required thermostat water bath when being used for producing CA199 protein's cell to resuscitate, its characterized in that: including thermostat water bath body, objective table (3), lead screw (9) and driving motor (8), be equipped with sealed end storehouse (5) and sump (6) on thermostat water bath body, lead screw (9) rotate locate thermostat water bath body on, and in sump (6) and with objective table (3) threaded connection are arranged in to one end, the other end stretches in sealed end storehouse (5) and with driving motor (8) pivot fixed connection, objective table (3) sliding connection is in sump (6), be equipped with a plurality of draw-in holes (4) that are used for pegging graft the cryopreservation pipe on objective table (3).
2. The thermostatic waterbath required for cell resuscitation for producing CA199 protein according to claim 1, wherein: be equipped with baffle (13) on the thermostat water bath body, baffle (13) divide into two with the inner chamber of thermostat water bath body, and correspond to form sealed end storehouse (5) and sump (6), lead screw (9) rotate connect on baffle (13), and be equipped with the sealing washer with between baffle (13).
3. The thermostatic waterbath required for cell resuscitation for producing CA199 protein according to claim 1, wherein: the object stage (3) is of a disc structure, an upward bulge (31) is arranged at the center of the object stage (3), the lead screw (9) is in threaded connection with the bulge (31), the outer wall of the objective table (3) is provided with a sliding block, the inner wall of the water sump (6) is provided with a sliding groove (61), and the sliding block is in sliding connection with the sliding groove (61).
4. A thermostat water bath as claimed in claim 3 for use in cell resuscitation for CA199 protein production, characterized in that: a connecting pipe (32) is arranged in the inner cavity of the bulge (31), and the lead screw (9) is in threaded connection with the connecting pipe (32).
5. The thermostatic waterbath for use in resuscitating cells producing CA199 protein as set forth in claim 4, wherein: the screw rod (9) comprises a polish rod part and a screw rod part, the polish rod part is rotationally connected to the partition plate (13) through a bearing, and the screw rod part is in threaded connection with the connecting pipe (32).
6. The thermostatic waterbath required for cell resuscitation for producing CA199 protein according to claim 1, wherein: a fixed support (11) is arranged in the sealed bottom bin (5), and a screw rod (9) is rotationally connected to the fixed support (11).
7. The thermostatic waterbath required for cell resuscitation for producing CA199 protein according to claim 1, wherein: a rubber fixing ring (41) is arranged in the clamping hole (4).
8. The thermostatic waterbath required for cell resuscitation for producing CA199 protein according to claim 1, wherein: the top end of the water bin (6) is covered with a transparent glass cover (2).
9. The thermostatic waterbath required for cell resuscitation for producing CA199 protein according to claim 1, wherein: the side wall of the water bath water storage bin (6) is provided with a window for observing the water level.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322264099.3U CN220665296U (en) | 2023-08-23 | 2023-08-23 | A required constant temperature water bath for producing CA199 protein's cell is resuscitated |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322264099.3U CN220665296U (en) | 2023-08-23 | 2023-08-23 | A required constant temperature water bath for producing CA199 protein's cell is resuscitated |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220665296U true CN220665296U (en) | 2024-03-26 |
Family
ID=90333495
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322264099.3U Active CN220665296U (en) | 2023-08-23 | 2023-08-23 | A required constant temperature water bath for producing CA199 protein's cell is resuscitated |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220665296U (en) |
-
2023
- 2023-08-23 CN CN202322264099.3U patent/CN220665296U/en active Active
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