CN220634032U

CN220634032U - Microgravity culture nutrient solution preparing device

Info

Publication number: CN220634032U
Application number: CN202321746243.0U
Authority: CN
Inventors: 张京军; 龚长华; 奚晓鹏; 迟海鹏
Original assignee: Beijing Dynaflow Experiment Technology Co Ltd
Current assignee: Beijing Dynaflow Experiment Technology Co Ltd
Priority date: 2023-07-05
Filing date: 2023-07-05
Publication date: 2024-03-22
Anticipated expiration: 2033-07-05

Abstract

The utility model provides a microgravity nutrient solution preparation device, which relates to the technical field of cell culture nutrient solution preparation and comprises a mixing tank for mixing and stirring nutrient solutions, wherein an air inlet pipe is arranged on the tank top of the mixing tank in a penetrating manner, a bottom shell is fixedly connected with the tank bottom in the mixing tank, a connecting piece is fixedly connected with the bottom end of the air inlet pipe, a rotating ring is rotatably connected with the outer wall of the connecting piece, a plurality of check valves which are opened into the mixing tank are arranged on the rotating ring in a penetrating manner, the outer side edge of the rotating ring is rotatably connected with the inner wall of the bottom shell, a connecting plate is fixedly connected with the inner side wall of the connecting piece, and a waterproof and breathable film is arranged so as to prevent liquid in the mixing tank from entering the air inlet pipe or the bottom shell when the check valve is opened due to air blowing.

Description

Microgravity culture nutrient solution preparing device

Technical Field

The utility model relates to the technical field of preparation of cell culture nutrient solution, in particular to a microgravity culture nutrient solution preparation device.

Background

Cell culture is a method for simulating in-vivo environment in vitro to enable the cells to survive, grow, reproduce and maintain main structures and functions, and researches find that, aiming at the environment of cell culture, the cells are placed in a microgravity environment to enable the tissue obtained by culture to be more similar to living tissue, and the microgravity is used for culturing the cells, which is similar to the traditional culture dish used for culturing the cells, and nutrient solution without components and functions is added into the cells according to actual conditions.

For example, a nutrient solution preparing device in the prior art (patent publication No. CN 215654823U) is characterized in that a control rod is fixedly connected to the inside of a device main body, a rotating shaft is fixedly connected to the bottom of the control rod, a driving device is fixedly connected to the bottom of the rotating shaft, and stirring rods are fixedly connected to the two sides of the control rod, so that the nutrient solution entering the device main body is mixed and stirred;

however, the stirring and mixing effects in the prior art are poor because: the stirring rod can only realize stirring and mixing the nutrient solution of corresponding degree of depth, and other deep nutrient solutions just need to be mixed after stirring to stirring rod department through nutrition, so we propose a microgravity culture nutrient solution configuration device that stirring effect is better.

Disclosure of Invention

The utility model aims to solve the problems in the prior art and provides a microgravity nutrient solution preparation device.

In order to achieve the above purpose, the present utility model adopts the following technical scheme: the utility model provides a microgravity cultivates nutrient solution configuration device, includes the blending tank that is used for nutrient solution to mix the stirring, the tank deck of blending tank runs through and is provided with the intake pipe, and the tank bottom fixedly connected with drain pan in the blending tank, the bottom fixedly connected with connecting piece of intake pipe, the outer wall rotation of connecting piece is connected with the swivel, run through on the swivel and be provided with a plurality of check valves that open in the blending tank, and the lateral margin and the drain pan inner wall rotation of swivel are connected, connecting piece inside wall fixedly connected with connecting plate, the connecting plate top surface rotates and is connected with the fan through air current drive rotation, the flabellum axle of fan runs through the outside extension of connecting plate and fixedly connected with U type pole, the both ends of U type pole respectively with swivel bottom surface fixed connection.

Further: the air inlet pipe is fixed on the tank top of the mixing tank, and a plurality of one-way valves are also arranged on the surface of the air inlet pipe.

Further: the air inlet pipe is characterized in that an annular air bag for adjusting the aperture of the air outlet of the air inlet pipe is arranged at the bottom edge of the inner wall of the air inlet pipe, an air charging pipe is connected to the annular air bag, and one end of the air charging pipe extends to the outside of the mixing tank through the top end of the air inlet pipe.

Further: the top of the bottom shell is communicated with a telescopic air bag, the top edge of the telescopic air bag is fixedly connected with an extrusion plate sleeved on the air inlet pipe, and the extrusion plate is used for extruding and discharging nutrient solution in the mixing tank and cleaning the outer wall of the air inlet pipe.

Further: the connecting piece is fixedly connected with a guide plate for scraping nutrient solution on the surface of the swivel, and the guide plate is arranged at the discharge hole of the mixing tank.

Further: the extruding plate is positioned at the feeding hole of the tank top of the mixing tank and is also provided with a one-way valve which is opened into the mixing tank, and one opening end of the one-way valve is provided with a waterproof breathable film.

Compared with the prior art, the utility model has the advantages and positive effects that,

1. when the raw material liquid is positioned in the mixing tank, the air pump is used for connecting the air inlet pipe, the air pump is used for inflating the air inlet pipe, air enters the connecting piece and the bottom shell through the air inlet pipe, and enters the mixing tank through the air inlet pipe and the one-way valve on the swivel, a large amount of rising bubbles are generated in the liquid in the mixing tank, mixing of the liquid is realized, when the air enters the connecting piece, the fan is blown to rotate, the fan blade shaft of the fan drives the U-shaped rod to rotate, and the U-shaped rod drives the swivel to rotate, so that the bubbles blown out through the one-way valve on the swivel can uniformly cover the mixing tank, and further mixing uniformity is ensured.

2. According to the utility model, the one-way valves are arranged between the bottom shell and the telescopic air bags, one of the two one-way valves is opened into the bottom shell, the other one of the two one-way valves is opened into the telescopic air bags, when the air inlet pipe is used for air intake, air enters the telescopic air bags from the one-way valve opened into the bottom shell, the telescopic air bags are forced to be in a full-air state, the support of the extrusion plate is realized, when the air pump is used for extracting air in the mixing tank through the air inlet pipe, the air inlet pipe and the one-way valve on the swivel are automatically closed, the telescopic air bags are used for extracting air in the telescopic air bags under the action of the one-way valve opened into the telescopic air bags, the telescopic air bags are further dried, the extrusion plate is driven to descend, liquid in the mixing tank and liquid on the surface of the air inlet pipe are extruded, and the liquid is discharged from the water outlet on the side edge of the mixing tank.

Drawings

FIG. 1 is a schematic perspective view of a microgravity nutrient solution preparing device according to the present utility model;

FIG. 2 is a schematic cross-sectional view of FIG. 1 showing a microgravity nutrient solution preparing apparatus according to the present utility model;

FIG. 3 is an enlarged view of the microgravity nutrient solution preparing device shown in FIG. 2A.

Legend description: 1. a mixing tank; 2. a U-shaped rod; 3. an air inlet pipe; 4. an inflation tube; 5. a one-way valve; 6. a waterproof breathable film; 7. a telescopic air bag; 8. a bottom case; 9. a swivel; 10. a fan; 11. a connecting piece; 12. an extrusion plate; 13. a deflector; 14. an annular air bag; 15. and (5) connecting a plate.

Detailed Description

In order that the above objects, features and advantages of the utility model will be more clearly understood, a further description of the utility model will be rendered by reference to the appended drawings and examples. It should be noted that, in the case of no conflict, the embodiments of the present application and the features in the embodiments may be combined with each other.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present utility model, however, the present utility model may be practiced otherwise than as described herein, and therefore the present utility model is not limited to the specific embodiments of the disclosure that follow.

1-3, a microgravity nutrient solution configuration device comprises a mixing tank 1 for mixing and stirring nutrient solutions, wherein an air inlet pipe 3 is arranged on the tank top of the mixing tank 1 in a penetrating manner, a bottom shell 8 is fixedly connected to the tank bottom in the mixing tank 1, a connecting piece 11 is fixedly connected to the bottom end of the air inlet pipe 3, a swivel 9 is rotatably connected to the outer wall of the connecting piece 11, a plurality of check valves 5 which are opened into the mixing tank 1 are arranged on the swivel 9 in a penetrating manner, the outer side edge of the swivel 9 is rotatably connected with the inner wall of the bottom shell 8, a connecting plate 15 is fixedly connected to the inner side wall of the connecting piece 11, a fan 10 which is driven to rotate by air flow is rotatably connected to the top surface of the connecting plate 15, a fan blade shaft of the fan 10 extends outwards through the connecting plate 15 and is fixedly connected with a U-shaped rod 2, and two ends of the U-shaped rod 2 are respectively fixedly connected to the bottom surface of the swivel 9;

in addition: the air inlet pipe 3 is fixed on the tank top of the mixing tank 1, and a plurality of one-way valves 5 are also arranged on the surface of the air inlet pipe 3.

In the scheme, the method comprises the following steps: when raw and other materials liquid is located mixing tank 1, use air pump to connect intake pipe 3, aerify in to intake pipe 3 through the air pump, gas gets into connecting piece 11 and drain pan 8 through intake pipe 3, and in getting into mixing tank 1 through the check valve 5 on intake pipe 3 and the swivel 9, produce a large amount of rising bubbles in the liquid in mixing tank 1, realize the mixing of liquid, and when gas gets into in connecting piece 11, can blow fan 10 rotatory, the flabellum axle of fan 10 drives U type pole 2 rotatory, U type pole 2 drives swivel 9 rotatory, make the bubble that blows out through the check valve 5 on the swivel 9 can evenly cover in the mixing tank 1, and then guarantee the homogeneity of mixing.

Further, as shown in fig. 3: an annular air bag 14 for adjusting the aperture of the air outlet of the air inlet pipe 3 is arranged at the bottom edge of the inner wall of the air inlet pipe 3, the annular air bag 14 is connected with an air charging pipe 4, and one end of the air charging pipe 4 extends to the outside of the mixing tank 1 through the top end of the air inlet pipe 3;

in the scheme, the method comprises the following steps: the annular air bag 14 is inflated through the inflation tube 4, so that the annular air bag 14 is inflated to the bottom end of the air inlet tube 3, and then under the condition that the air inlet tube 3 is constant in air inflow, the air speed of blown air flow can be adjusted by changing the caliber of the air outlet end of the air inlet tube 3, the rotating speed of the fan 10 is controlled, the rotating speed of the fan 10 is adjusted, and the rotating speed of the swivel 9 is adjusted.

Further, as shown in fig. 2: the top of the bottom shell 8 is communicated with a telescopic air bag 7, the top edge of the telescopic air bag 7 is fixedly connected with an extrusion plate 12 sleeved on the air inlet pipe 3, and the extrusion plate 12 is used for extruding and discharging nutrient solution in the mixing tank 1 and cleaning the outer wall of the air inlet pipe 3;

in the scheme, the method comprises the following steps: the one-way valve 5 is also arranged between the bottom shell 8 and the telescopic air bag 7, two one of the one-way valves 5 is opened into the bottom shell 8, the other one of the one-way valves 5 is opened into the telescopic air bag 7, when the air inlet pipe 3 is in air inlet, air enters the telescopic air bag 7 from the one-way valve 5 opened into the bottom shell 8, the telescopic air bag 7 is forced to be in a full-air state, the support of the extrusion plate 12 is realized, when the air pump pumps air in the mixing tank 1 through the air inlet pipe 3, the one-way valve 5 on the air inlet pipe 3 and the swivel 9 is automatically closed, the telescopic air bag 7 realizes air extraction in the telescopic air bag 7 under the action of the one-way valve 5 opened into the telescopic air bag 7, further realizes the shrinkage of the telescopic air bag 7, drives the extrusion plate 12 to descend, realizes the extrusion of liquid in the mixing tank 1 and the liquid on the surface of the air inlet pipe 3 through the extrusion plate 12, and is discharged from the water outlet on the side edge of the mixing tank 1.

Further, as shown in fig. 2: a guide plate 13 for scraping nutrient solution on the surface of the swivel 9 is fixedly connected to the connecting piece 11, and the guide plate 13 is arranged at a discharge port of the mixing tank 1;

the guide plate 13 is arranged to suck air at the air pump, air passes through the connecting piece 11, and when the fan 10 in the connecting piece 11 is driven to rotate, the fan 10 drives the U-shaped rod 2 to rotate, the rotating ring 9 is rotated, when the rotating ring 9 passes through the guide plate 13, liquid attached to the surface of the rotating ring 9 is scraped through the scraping effect of the guide plate 13, and the liquid flows to the water outlet of the mixing tank 1 through the inclined guide plate 13.

Further, as shown in fig. 2: the extruding plate 12 is positioned at the top feed inlet of the mixing tank 1 and is also provided with a one-way valve 5 which is opened into the mixing tank 1, and one opening end of the one-way valve 5 is provided with a waterproof breathable film 6;

the waterproof and breathable membrane 6 is arranged so as to prevent liquid in the mixing tank 1 from entering the air inlet pipe 3 or the bottom shell 8 when the one-way valve 5 is opened due to the blowing of the gas.

The present utility model is not limited to the above-mentioned embodiments, and any equivalent embodiments which can be changed or modified by the technical content disclosed above can be applied to other fields, but any simple modification, equivalent changes and modification made to the above-mentioned embodiments according to the technical substance of the present utility model without departing from the technical content of the present utility model still belong to the protection scope of the technical solution of the present utility model.

Claims

1. The utility model provides a microgravity culture nutrient solution configuration device, includes the blending tank (1) that is used for nutrient solution mixing stirring, its characterized in that: the tank deck of hybrid tank (1) runs through and is provided with intake pipe (3), and tank bottom fixedly connected with drain pan (8) in hybrid tank (1), the bottom fixedly connected with connecting piece (11) of intake pipe (3), the outer wall rotation of connecting piece (11) is connected with swivel (9), run through on swivel (9) and be provided with a plurality of check valves (5) that open in hybrid tank (1), and the lateral side of swivel (9) and drain pan (8) inner wall rotation are connected, connecting piece (11) inside wall fixedly connected with connecting plate (15), connecting plate (15) top surface rotation is connected with fan (10) through the air current drive rotation, the fan blade axle of fan (10) runs through connecting plate (15) outside extension and fixedly connected with U type pole (2), the both ends of U type pole (2) respectively with swivel (9) bottom surface fixed connection.

2. The microgravity culture nutrient solution dispensing device of claim 1, wherein: the air inlet pipe (3) is fixed on the tank top of the mixing tank (1), and a plurality of one-way valves (5) are also arranged on the surface of the air inlet pipe (3).

3. The microgravity culture nutrient solution dispensing device of claim 1, wherein: the novel air inlet pipe is characterized in that an annular air bag (14) for adjusting the aperture of the air outlet of the air inlet pipe (3) is arranged at the bottom edge of the inner wall of the air inlet pipe (3), an air charging pipe (4) is connected to the annular air bag (14), and one end of the air charging pipe (4) extends to the outside of the mixing tank (1) through the top end of the air inlet pipe (3).

4. The microgravity culture nutrient solution dispensing device of claim 1, wherein: the utility model discloses a liquid mixing tank, including drain pan (8), telescopic gasbag (7), extrusion board (12) on intake pipe (3) are established to topside fixedly connected with cover of telescopic gasbag (7), extrusion board (12) are arranged in extrusion discharge blending tank (1) nutrient solution, and are arranged in the clearance intake pipe (3) outer wall.

5. The microgravity culture nutrient solution dispensing device of claim 1, wherein: the connecting piece (11) is fixedly connected with a guide plate (13) for scraping nutrient solution on the surface of the swivel (9), and the guide plate (13) is arranged at the discharge port of the mixing tank (1).

6. The microgravity culture nutrient solution dispensing device of claim 4 wherein: the extruding plate (12) is positioned at the tank top feed inlet of the mixing tank (1) and is also provided with a one-way valve (5) which is opened into the mixing tank (1), and one opening end of the one-way valve (5) is provided with a waterproof breathable film (6).