CN220597519U

CN220597519U - Cell culture device

Info

Publication number: CN220597519U
Application number: CN202321775704.7U
Authority: CN
Inventors: 杨莉丽; 黎子彤; 王雪雯
Original assignee: Zhuhai Xiborui Biotechnology Co ltd
Current assignee: Zhuhai Xiborui Biotechnology Co ltd
Priority date: 2023-07-07
Filing date: 2023-07-07
Publication date: 2024-03-15
Anticipated expiration: 2033-07-07

Abstract

The utility model discloses a cell culture device, which comprises an equipment box and a nutrient solution box, wherein the equipment box and the nutrient solution box are connected from top to bottom, the equipment box is positively hinged with a switch door, a plurality of partition plates are connected and arranged in the equipment box, and the partition plates divide the equipment box into a plurality of culture boxes, a plurality of refrigeration boxes and a temperature control box, and compared with the prior art, the cell culture device has the advantages that: 1. the temperature sensor, the heating layer, the fan motor, the fan blade, the air refrigerator and the refrigerating tube are arranged, the temperature of the environment for cell growth can be adjusted, the environment is at a proper growth temperature, the cell growth quality is enhanced, the nutrient solution box, the input pump, the main output tube, the branch output tube, the control switch valve and the liquid injection head are arranged, the inside of the nutrient solution box is not required to be supplemented manually, the artificial load is reduced, the observation window is arranged, the cell growth condition can be roughly observed in the external environment, the condition of a frequent opening device is reduced, and the probability of cell death is reduced.

Description

Cell culture device

Technical Field

The utility model relates to the technical field of cell culture tools, in particular to a cell culture device.

Background

Cell culture refers to the process of simulating in-vivo environment in-vitro environment to enable cells to survive, grow and reproduce, has great significance to industries such as biopharmaceuticals, life sciences and drug research, is an indispensable process, and is characterized in that basic cells are required to be placed in a culture dish in the cell culture process, nutrient solution is dripped into the culture dish in an environment which can be sterilized and has proper temperature to enable the cells to grow, and then the nutrient solution is required to be dripped into the culture dish in a manual timing mode in the process, so that the artificial load is increased, the cells are easy to die in the process of continuously opening the culture device, the cells are required to be observed in a timing mode in the cell culture process, the culture device is required to be opened for observation in the traditional state, and the cell death is easy to be caused by temperature change in the process.

Disclosure of Invention

The technical problem to be solved by the utility model is to overcome the problems, and design a cell culture device which can be used for semi-automatically injecting nutrient solution into a culture dish and observing the culture dish in the external environment.

In order to solve the technical problems, the technical scheme provided by the utility model is as follows: the utility model provides a cell culture device, includes equipment box and nutrient solution case, equipment box and nutrient solution case top-down connect the setting, equipment box forward articulated is equipped with the switch door, equipment box internal connection is equipped with a plurality of division boards, the division board separates equipment box and is equipped with a plurality of incubator, a plurality of refrigeration case and accuse temperature case, the incubator is all connected and is equipped with a plurality of fixing bases, the culture dish has all been placed to the fixing base, the incubator is all connected and is equipped with the notes liquid head of a plurality of cooperation culture dishes, the nutrient solution case is equipped with the nutrient solution mouth, nutrient solution incasement portion is equipped with the input pump, the input pump is connected and is equipped with main output tube, main output tube connection is equipped with the branch output tube of cooperation notes liquid head, branch output tube and a plurality of notes liquid head connect the setting, branch output tube all connects and is equipped with the control switch valve.

As an improvement, the equipment box and the switch door are connected together and provided with hinges which are hinged in a matched mode.

As an improvement, the switch door is provided with a plurality of observation windows matched with the incubator, so that the switch door can be observed outside to observe the growth area and the state of cells.

As an improvement, the incubator is internally connected with a temperature sensor, the incubator is connected with a heating layer provided with a matched incubator, the incubator is connected with a fan motor provided with the matched heating layer, the fan motor is connected with a fan shaft, the fan shaft is connected with fan blades, the temperature inside the incubator can be detected, and if the temperature is lower than the rated temperature, the incubator is heated to the environment, so that the cell growth speed is prevented from slowing down.

As an improvement, the inside connection of refrigeration case is equipped with the air cooler, the equal connection of air cooler is equipped with the refrigeration pipe, can cool down the processing to the internal environment, prevents that the high temperature from leading to cell death.

As an improvement, the fixing base is connected and is provided with a first magnetic block, the culture dish is connected and is provided with a second magnetic block matched with the first magnetic block, the stability of the fixing base and the culture dish is enhanced, and toppling is prevented.

As an improvement, the incubator is connected with a plurality of illumination lamps, and can selectively carry out supplementary illumination culture according to the type of the cultured cells.

As an improvement, a controller is connected with the outer side of the equipment box.

Compared with the prior art, the utility model has the advantages that:

1. the cell culture device is provided with a temperature sensor, a heating layer, a fan motor, a fan blade, an air refrigerator and a refrigerating tube, and can adjust the temperature of the cell growth environment to enable the cell growth environment to be at a proper growth temperature, so that the cell growth quality is enhanced.

2. The cell culture device is provided with a nutrient solution box, an input pump, a main output pipe, a branch output pipe, a control switch valve and a liquid injection head, and the inside of the cell culture device is not required to be supplemented manually, so that the manual load is reduced.

3. The cell culture device is provided with the observation window, so that the growth condition of cells can be roughly observed in the external environment, the condition of frequently opening the device is reduced, and the probability of cell death is reduced.

Drawings

FIG. 1 is a schematic diagram of a cell culture apparatus according to the present utility model.

FIG. 2 is a schematic view showing the appearance of a cell culture apparatus according to the present utility model.

FIG. 3 is a side view of the appearance of a cell culture apparatus according to the utility model.

FIG. 4 is a schematic diagram showing the structure of the main output pipe, branch output pipe, control switch valve and liquid injection head of the cell culture apparatus according to the present utility model.

FIG. 5 is a schematic diagram showing the structure of a holder and a culture dish of a cell culture apparatus according to the present utility model.

As shown in the figure: 1. an equipment box; 2. a nutrient solution tank; 3. a partition plate; 4. an incubator; 5. a refrigeration box; 6. a temperature control box; 7. a fixing seat; 8. a culture dish; 9. a nutrient solution port; 10. an input pump; 11. a main output pipe; 12. a branch output pipe; 13. controlling a switch valve; 14. a liquid injection head; 15. a temperature sensor; 16. a hinge; 17. a fan motor; 18. a fan shaft; 19. a fan blade; 20. a heating layer; 21. a lighting lamp; 22. an air refrigerator; 23. a refrigeration tube; 24. a first magnetic block; 25. a second magnetic block; 26. opening and closing a door; 27. an observation window; 28. and a controller.

Detailed Description

The present utility model will be described in further detail with reference to the accompanying drawings.

Referring to fig. 1-5, a cell culture apparatus comprises a device box 1 and a nutrient solution box 2, wherein the device box 1 and the nutrient solution box 2 are connected from top to bottom, a controller 28 is connected and arranged on the outer side of the device box 1, a switch door 26 is arranged on the forward hinge of the device box 1, a plurality of observation windows 27 matched with the incubator 4 are arranged on the switch door 26, a hinge 16 matched with the hinge is arranged on the joint of the device box 1 and the switch door 26, a plurality of partition plates 3 are arranged on the inner connection of the device box 1, the partition plates 3 divide the device box 1 into a plurality of incubators 4, a plurality of refrigeration boxes 5 and a temperature control box 6, the incubators 4 and the refrigeration boxes 5 are arranged from top to bottom one by one, the temperature control box 6 is arranged on the right side of the plurality of incubators 4 and the plurality of refrigeration boxes 5, a plurality of fixing seats 7 are connected and arranged on the incubators 4, a culture dish 8 is arranged on the fixing seats 7, the culture dish 8 is made of transparent materials, the fixed seat 7 is connected with a first magnetic block 24, the culture dish 8 is connected with a second magnetic block 25 matched with the first magnetic block 24, the first magnetic block 24 and the second magnetic block 25 are opposite, the culture box 4 is connected with a plurality of illuminating lamps 21, the culture box 4 is connected with a plurality of liquid injection heads 14 matched with the culture dish 8, the nutrient solution box 2 is provided with a nutrient solution port 9, an input pump 10 is arranged in the nutrient solution box 2, the input pump 10 is connected with a main output pipe 11, the main output pipe 11 is connected with a branch output pipe 12 provided with a matched liquid injection head 14, the branch output pipe 12 is connected with a plurality of liquid injection heads 14, the branch output pipe 12 is connected with a control switch valve 13, the interior of the culture box 4 is connected with a temperature sensor 15, the temperature control box 6 is connected with a heating layer 20 provided with a matched culture box 4, the temperature control box 6 is connected and is equipped with the fan motor 17 of cooperation zone of heating 20, fan motor 17 all connects and is equipped with fan shaft 18, fan shaft 18 all connects and is equipped with fan blade 19, the inside all connection of refrigeration case 5 is equipped with air cooler 22, air cooler 22 all connects and is equipped with refrigeration pipe 23, and all electric devices in this device all are external to be equipped with the power, and this is common prior art so not too much to describe.

In the specific implementation of the utility model, the nutrient solution for cell culture is injected into the nutrient solution tank 2 through the nutrient solution port 9, cells to be cultured are put into the culture dish 8, basic nutrient solution is injected into the inside, the culture dish 8 is placed above the fixed seat 7, the culture dish is stabilized by the first magnetic block 24 and the second magnetic block 25, the switch door 26 is closed, the rated temperature is set by the controller 28, when the internal temperature is too low, the heating layer 20 and the fan motor 17 work, the growing environment of the culture dish 8 is heated, after the rated temperature is reached, the fan motor 17 and the heating layer 20 stop working, when the internal temperature is too high, the air refrigerator 22 and the refrigerating pipe 23 work, the temperature reduction treatment is carried out on the inside, after the temperature is reduced to the rated temperature, the air refrigerator 22 stops working, when the nutrient solution is required to be supplemented in the culture dish 8, the input pump 10 works, the nutrient solution is extracted, the control switch valve 13 corresponding to the culture dish 8 which is required to be supplemented with the nutrient solution is opened through the controller 28, the nutrient solution is dripped into the culture dish 8 through the liquid injection head 14, the corresponding culture dish 8 is supplemented, when the growth condition of the culture dish 8 is required to be observed, the cell growth condition in the culture dish 8 can be observed through the observation window 27, the cell coverage area is large under good growth condition, the cell coverage area is small under poor growth condition, and the growth condition is roughly observed under comparison area.

The utility model and its embodiments have been described above with no limitation, and the actual construction is not limited to the embodiments of the utility model as shown in the drawings. In summary, if one of ordinary skill in the art is informed by this disclosure, a structural manner and an embodiment similar to the technical solution should not be creatively devised without departing from the gist of the present utility model.

Claims

1. Cell culture apparatus, including equipment box (1) and nutrient solution case (2), its characterized in that: the utility model provides a nutrient solution box, including equipment box (1), nutrient solution box (2), equipment box (1) and nutrient solution box (2) top-down connect the setting, equipment box (1) forward articulated is equipped with switch door (26), equipment box (1) internally connected is equipped with a plurality of division boards (3), division board (3) separate equipment box (1) are equipped with a plurality of incubator (4), a plurality of refrigeration case (5) and accuse temperature case (6), incubator (4) all connect and are equipped with a plurality of fixing base (7), culture dish (8) are all placed to fixing base (7), incubator (4) all connect and are equipped with annotate liquid head (14) of a plurality of cooperation culture dish (8), nutrient solution box (2) are equipped with nutrient solution mouth (9), nutrient solution box (2) inside is equipped with input pump (10), input pump (10) connect and are equipped with main output tube (11), main output tube (11) connect and are equipped with branch output tube (12) of cooperation annotate liquid head (14), branch output tube (12) and a plurality of annotate liquid head (14) and connect the setting, branch output tube (12) connect and are equipped with switch valve (13).

2. A cell culture apparatus according to claim 1, wherein: the equipment box (1) and the switch door (26) are connected together and provided with a hinge (16) which is hinged in a matched mode.

3. A cell culture apparatus according to claim 1, wherein: the switch door (26) is provided with a plurality of observation windows (27) matched with the incubator (4).

4. A cell culture apparatus according to claim 1, wherein: the incubator is characterized in that temperature sensors (15) are connected and arranged in the incubator (4), the temperature control box (6) is connected and provided with a heating layer (20) matched with the incubator (4), the temperature control box (6) is connected and provided with a fan motor (17) matched with the heating layer (20), the fan motor (17) is connected and provided with a fan shaft (18), and the fan shaft (18) is connected and provided with fan blades (19).

5. A cell culture apparatus according to claim 1, wherein: the inside of the refrigerating box (5) is connected with an air refrigerator (22), and the air refrigerator (22) is connected with a refrigerating pipe (23).

6. A cell culture apparatus according to claim 1, wherein: the fixed seat (7) is connected and is equipped with first magnetic path (24), culture dish (8) are connected and are equipped with second magnetic path (25) of cooperation first magnetic path (24).

7. A cell culture apparatus according to claim 1, wherein: the incubator (4) is connected with a plurality of illuminating lamps (21).

8. A cell culture apparatus according to claim 1, wherein: the outside of the equipment box (1) is connected with a controller (28).