CN220588412U

CN220588412U - Experimental animal heart perfusion device and system

Info

Publication number: CN220588412U
Application number: CN202321733959.7U
Authority: CN
Inventors: 张汝涵; 刘芊言; 刘密; 李祖强
Original assignee: Hunan University of Chinese Medicine
Current assignee: Hunan University of Chinese Medicine
Priority date: 2023-07-04
Filing date: 2023-07-04
Publication date: 2024-03-15
Anticipated expiration: 2033-07-04

Abstract

The utility model provides a heart perfusion device and a heart perfusion system for experimental animals, wherein the device comprises: the connecting pipe assembly comprises at least two connecting branch pipes and a connecting main pipe, each connecting branch pipe is respectively communicated with one end of the connecting main pipe, and a needle head is detachably arranged at the other end of the connecting main pipe; the flow velocity adjusting parts comprise at least two flow velocity adjusting parts which are respectively arranged on each connecting branch pipe; and the flow control assembly is arranged on the connecting main pipe. According to the injection needle tube, the plurality of connecting branch tubes are arranged to be connected with the connecting main tube, so that various liquid medicines respectively enter the connecting main tube for injection, quantitative injection and extraction are avoided each time when the injection needle tube is used, and the workload is high; the flow control assembly is arranged to control and monitor the flow of the connecting main pipe, so that corresponding quantitative injection can be realized according to different animal body types each time, continuous injection of a plurality of different experimental animals can be realized, the injection medicine amount is more accurate, and the experimental effect is ensured.

Description

Experimental animal heart perfusion device and system

Technical Field

The utility model belongs to the field of injection devices, and particularly relates to a heart perfusion device and system for experimental animals.

Background

In experimental animal histopathological studies, fixation is usually performed prior to animal sacrifice in order to avoid or reduce post-mortem changes in tissue morphology and structure. The fixing method has various methods, namely perfusion fixing is the most common method, namely, perfusion liquid is enabled to be filled in tissues rapidly and uniformly through blood circulation by ventricular perfusion fixing liquid, living cells are fixed in situ uniformly, and the method has important significance for maintaining the integrity of tissue cells.

In animal experimental sampling, which currently requires cardiac perfusion, it may be necessary to perform cardiac perfusion on a plurality of different species of experimental animals simultaneously. According to the sizes of different experimental animals, corresponding to 50-500ML of different syringes, respectively extracting different milliliters of liquid medicine (usually normal saline and paraformaldehyde) and respectively injecting, wherein the workload of the extraction and injection steps is large and troublesome; when the injection is carried out through the infusion bottle injection mode, a user needs to pay attention to the scales of the infusion bottle at any time to observe the injection dosage, so that excessive injection or insufficient injection quantity is avoided.

Disclosure of Invention

The utility model aims to solve the technical problem of providing an experimental animal heart perfusion device so as to solve the problem that heart perfusion is possibly required to be carried out on a plurality of different experimental animals simultaneously in the existing animal experimental materials needing heart perfusion. According to the sizes of different experimental animals, corresponding to 50-500ML of different syringes, respectively extracting different milliliters of liquid medicine (usually normal saline and paraformaldehyde) and respectively injecting, wherein the workload of the extraction and injection steps is large and troublesome; when the infusion is performed through the infusion mode, a user needs to pay attention to the scales of the infusion bottle at any time to observe the infusion dose, so that the problems of excessive infusion or insufficient infusion quantity are avoided.

In order to achieve the above object, the present application adopts a technical scheme that provides a heart perfusion device for experimental animals, including:

the connecting pipe assembly comprises at least two connecting branch pipes and a connecting main pipe, each connecting branch pipe is respectively communicated with one end of the connecting main pipe, and a needle head is detachably arranged at the other end of the connecting main pipe;

the flow velocity adjusting parts comprise at least two flow velocity adjusting parts, and each flow velocity adjusting part is respectively arranged on each connecting branch pipe;

and the flow control assembly is arranged on the connecting main pipe and is used for setting and controlling the quantity of single injection.

The flow control assembly comprises a drip cup, a drip speed sensor, a monitoring piece and an opening and closing valve, wherein the drip speed sensor is arranged on one side of the drip cup, the monitoring piece is arranged on the connecting main pipe, and the opening and closing valve is arranged at the joint of the monitoring piece and the connecting main pipe;

the drip speed sensor and the on-off valve are electrically connected with the monitoring member, respectively.

The dripping speed sensor comprises a transmitting antenna and a receiving antenna, wherein the transmitting antenna transmits incident microwaves to liquid drops in the dripping kettle according to preset time intervals; the receiving antenna receives a reflected wave of the falling reflection of the liquid drop.

The monitoring piece comprises a display screen, a control button and a controller, wherein the control button, the display screen, the dripping speed sensor and the opening and closing valve are all electrically connected with the controller.

Wherein the connection main pipe is detachably connected with the flow control assembly.

And each connecting branch pipe is provided with a branch pipe drip cup, and the branch pipe drip cup is arranged on the connecting branch pipe between the flow speed adjusting piece and the connecting main pipe.

Wherein, each connecting branch pipe is provided with a puncture outfit at one end deviating from the connecting main pipe.

The experimental animal heart perfusion system comprises an infusion support, an infusion bottle and the experimental animal heart perfusion device, wherein the infusion bottle is hung on the upper side of the infusion support, and a puncture outfit in the experimental animal heart perfusion device is connected with the infusion bottle.

The infusion support comprises a telescopic support, a base and an infusion bottle hook, wherein the base and the infusion bottle hook are respectively arranged at two ends of the telescopic support, and a roller is arranged at one end, deviating from the telescopic support, of the base.

The utility model has the beneficial effects that the plurality of connecting branch pipes are arranged to be connected with one connecting main pipe, and the plurality of connecting branch pipes are connected with the liquid medicine, so that various liquid medicines respectively enter the connecting main pipe for injection, quantitative injection and extraction are avoided each time when the injection needle tube is used, the workload is larger, and the operation is more troublesome; in addition, through setting up flow control subassembly and controlling the flow of monitoring to connecting the person in charge, guaranteed that can realize corresponding ration injection according to different animal sizes at every turn, can realize injecting many different experimental animals in succession, and injection medicine quantity is more accurate, has guaranteed experimental effect.

Drawings

Fig. 1 is a schematic structural diagram of an experimental animal heart perfusion device according to an embodiment of the present utility model;

fig. 2 is a schematic circuit diagram of an experimental animal heart perfusion device according to an embodiment of the present utility model.

The reference numerals in the drawings:

10-a connecting tube assembly; 11-connecting branch pipes; 111-branch pipe drip cup; 112-puncture outfit; 12-connecting a main pipe; 13-a needle; 20-a flow rate regulator; 30-a flow control assembly; 31-drip cup; 32-drip speed sensor; 33-monitoring piece; 331-a display screen; 332-control buttons; 333-a controller; 34-opening and closing valves; 40-an infusion support; 41-a telescopic bracket; 42-a base; 43-infusion bottle hook; 44-a roller; 50-infusion bottle.

Detailed Description

In order to make the objects, technical solutions and advantages of the present utility model more apparent, the technical solutions in the preferred embodiments of the present utility model will be described in more detail with reference to the accompanying drawings in the preferred embodiments of the present utility model. In the drawings, the same or similar reference numerals refer to the same or similar components or components having the same or similar functions throughout. The described embodiments are some, but not all, embodiments of the utility model. The embodiments described below by referring to the drawings are illustrative and intended to explain the present utility model and should not be construed as limiting the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model. Embodiments of the present utility model will be described in detail below with reference to the accompanying drawings.

In the description of the present utility model, it should be noted that, unless explicitly stated and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be fixedly connected, or indirectly connected through intermediaries, for example, or may be in communication with each other between two elements or in an interaction relationship between the two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

In the description of the present utility model, it should be understood that the terms "upper," "lower," "front," "rear," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present utility model and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.

The terms "first," "second," and "third" in the description and claims of the present application and in the above-described figures, if any, are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate such that embodiments of the present application described herein may be capable of operation in sequences other than those illustrated or described herein, for example.

Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or maintenance tool that comprises a list of steps or elements is not necessarily limited to those steps or elements that are expressly listed or inherent to such process, method, article, or maintenance tool.

As shown in fig. 1-2, the present utility model provides a heart perfusion device for experimental animals, comprising: the connecting pipe assembly 10 comprises at least two connecting branch pipes 11 and a connecting main pipe 12, wherein each connecting branch pipe 11 is respectively communicated with one end of the connecting main pipe 12, and a needle 13 is detachably arranged at the other end of the connecting main pipe 12; the flow rate adjusting member 20 includes at least two flow rate adjusting members 20, each flow rate adjusting member 20 being provided on each connecting branch pipe 11, respectively; a flow control assembly 30 is provided on the connection main 12, and the flow control assembly 30 is used to set and control the amount of a single injection.

The connection branch pipes 11 may be provided in plural numbers, and the type of infusion is specifically selected according to the need, and in this application, two connection branch pipes 11 are provided for illustration. Specifically, in the heart perfusion process of the experimental animal, according to the size of the experimental animal, 50-500ML unequal injectors are usually required to be selected, and physiological saline and paraformaldehyde are respectively extracted for injection. For this reason, in the present application, two infusion bottles are provided, which are loaded with physiological saline and paraformaldehyde, respectively, so that simultaneous injection is facilitated.

Correspondingly, the two connecting branch pipes 11 are connected with infusion bottles, and the liquid medicine in the infusion bottles enters the connecting branch pipes 11 and is led into the connecting main pipe 12 through the two connecting branch pipes 11. It should be noted that the two connecting branch pipes 11 are provided with flow rate adjusting members 20, and the flow rate and opening and closing of the connecting branch pipes 11 are adjusted by the flow rate adjusting members 20, thereby realizing that two kinds of liquid medicine are injected into the connecting main pipe 12 at intervals. The flow rate adjusting member 20 may be a flow rate adjuster with a pulley, and may be capable of adjusting a flow rate, which is not limited in this embodiment.

Taking an experimental mouse as an example, 50ml of physiological saline and 50ml of paraformaldehyde are needed to be injected during injection, firstly 50ml of physiological saline is injected, then 50ml of paraformaldehyde is injected, at the moment, the flow speed adjusting piece 20 of the connecting branch pipe 11 corresponding to one side of the physiological saline is adjusted to a certain opening degree, the flow speed adjusting piece 20 of the connecting branch pipe 11 corresponding to the other side of the paraformaldehyde is adjusted to be closed, and at the moment, the physiological saline firstly enters the connecting branch pipe 11 for injection; after the injection is completed, the flow rate adjusting member 20 on the physiological saline side is closed, and the flow rate adjusting member 20 on the paraformaldehyde side is opened, thereby realizing the subsequent injection of paraformaldehyde. It should be noted that, in the present application, synchronous injection may be implemented, that is, after the liquid medicine is mixed, the opening of the flow rate adjusting member 20 is adjusted to implement mixed injection of the liquid medicine in different proportions, which is not limited in this embodiment.

In addition, a flow control assembly 30 is further provided on the connection main pipe 12, and the flow control assembly 30 monitors and controls the flow rate of the liquid in the unit time of the connection main pipe 12, thereby realizing quantitative injection of the liquid medicine.

When the medical liquid connecting device is used, the connecting branch pipes 11 are respectively connected with different medical liquids, and the flow rate of the corresponding medical liquid is controlled through the flow rate adjusting piece 20, so that the proportion of the medical liquid entering the connecting main pipe 12 is controlled. At this time, the needle 13 is inserted into the body of the experimental animal, the flow rate adjusting part 20 of the connecting branch pipe 11 corresponding to one side of the physiological saline is adjusted to a certain opening, the flow rate adjusting part 20 of the connecting branch pipe 11 corresponding to the other side of the paraformaldehyde is adjusted to be closed, at this time, the physiological saline firstly enters the connecting branch pipe 11, and the single injection dosage, namely 50ml physiological saline is set through the flow control assembly 30, and the first injection is carried out; after the injection is completed, the flow rate regulating element 20 at one side of the normal saline is closed, the flow rate regulating element 20 at one side of the paraformaldehyde is opened, at the moment, the paraformaldehyde enters the connecting branch pipe 11, and the dosage of the single injection, namely 50ml of paraformaldehyde is set through the flow control assembly 30 to carry out the second injection; after the quantitative injection is finished, the flow control assembly 30 is automatically closed, and the injection can be finished, and the needle 13 is pulled out at the moment.

According to the injection needle tube, the plurality of connecting branch pipes 11 are arranged to be connected with one connecting main tube 12, and the plurality of connecting branch pipes 11 are connected with liquid medicine, so that various liquid medicines respectively enter the connecting main tube 12 for injection, quantitative injection and extraction are required each time when the injection needle tube is used, the workload is large, and the operation is troublesome; in addition, through setting up flow control assembly 30 and controlling the flow of monitoring to connecting person in charge 12, can realize injecting many different experimental animals in succession, and injection medicine dose is more accurate, has guaranteed experimental effect.

In order to achieve the experimental effect of quantitative control injection, optionally, the flow control assembly 30 includes a drip cup 31, a drip speed sensor 32, a monitor 33, and an on-off valve 34, the drip speed sensor 32 is disposed on one side of the drip cup 31, the monitor 33 is disposed on the connecting main pipe 12, and the on-off valve 34 is disposed at the junction of the monitor 33 and the connecting main pipe 12; the drip sensor 32 and the on-off valve 34 are electrically connected to the monitor 33, respectively.

The drip speed sensor 32 is used for detecting the drip speed of the liquid medicine in the drip cup 31, so that the flow in unit time is judged according to the drip speed, the flow is detected and controlled through the monitoring piece 33, and when the flow of the liquid medicine flowing out of the flow control assembly 30 reaches a set value, the monitoring piece 33 controls the opening and closing valve 34 to be closed, so that single injection can be realized.

In this embodiment, specifically, the drip speed sensor 32 includes a transmitting antenna and a receiving antenna, the transmitting antenna transmitting incident microwaves toward the drip in the drip cup 31 at preset time intervals; the receiving antenna receives the reflected wave reflected by the liquid drop falling, and the Doppler effect shows that the fixed frequency microwave emitted by the emitting antenna meets the reflected wave of a static object, the frequency of the reflected wave is not changed, the Doppler frequency shift can be generated when the reflected wave meets the liquid drop falling moving object, and the liquid drop falling speed can be judged by the change of the frequency. The microwave signal is transmitted while the reflected signal of the liquid drop is received, and the two signals are mixed and the difference frequency generates a new low-frequency signal, which is called Doppler signal, the frequency of which is Doppler frequency and is the difference between the transmitting frequency and the reflecting frequency. The monitor 33 detects this doppler signal to count the number of drops falling per unit time, thereby obtaining the drop velocity. Accordingly, the flow rate may be detected by other means, which is not limited in this embodiment.

In this application, specifically, the monitor 33 includes a display screen 331, a control button 332 and a controller 333, the control button 332, the display screen 331, the drip speed sensor 32 and the on-off valve 34 are all electrically connected with the controller 333, an instruction is sent to the controller 333 through the control button 332, the amount of single injection is set, the controller 333 displays the amount on the display screen 331 and controls the on-off valve 34 to open and close, the controller 333 calculates in combination with timing and the drip speed detected by the drip speed sensor 32, and when the amount of injection reaches 50ml, the controller 333 controls the on-off valve 34 to close, so that automatic injection can be realized.

In addition, in order to be convenient for change the consumptive material, in this application, optionally, connect the person in charge 12 and flow control assembly 30 can be dismantled and be connected to be convenient for change the person in charge 12, make flow control assembly 30 can cyclic utilization.

Optionally, a branch drip cup 111 is provided on each connection branch 11, the branch drip cup 111 is provided on the connection branch 11 between the flow velocity adjusting member 20 and the connection main pipe 12, and the flow velocity of the branch can be determined by the branch drip cup 111; in addition, two flow control assemblies 30 may be provided, and the two flow control assemblies 30 are provided on the two connection branches 11, respectively, so that control of the individual branches is achieved, which is not limited in this embodiment.

In addition, in order to facilitate connection of the connection branch pipes 11 with the infusion bottle, a puncture outfit 112 may be optionally provided at one end of each connection branch pipe 11 facing away from the connection main pipe 12.

The application also provides an experimental animal heart perfusion system, which comprises an infusion support 40, an infusion bottle 50 and the experimental animal heart perfusion device, wherein the infusion bottle 50 is hung on the upper side of the infusion support 40, and a puncture outfit 112 in the experimental animal heart perfusion device is connected with the infusion bottle 50.

The experimental animal heart perfusion device has the same structure as the experimental animal heart perfusion device in the above embodiment, and the technical effects are the same, and therefore, the description thereof is omitted.

In addition, in order to realize the overall convenience of the system, optionally, the infusion support 40 includes a telescopic support 41, a base 42 and an infusion bottle hook 43, the base 42 and the infusion bottle hook 43 are respectively disposed at two ends of the telescopic support 41, and a roller 44 is disposed at one end of the base 42 facing away from the telescopic support 41.

Those of ordinary skill in the art will appreciate that: the discussion of any of the embodiments above is merely exemplary and is not intended to imply that the scope of the present application is limited to such examples; the technical features of the above embodiments or in the different embodiments may also be combined under the idea of the present application, the steps may be implemented in any order, and there are many other variations of the different aspects of one or more embodiments in the present application as above, which are not provided in details for the sake of brevity.

One or more embodiments herein are intended to embrace all such alternatives, modifications and variations that fall within the broad scope of the present application. Any omissions, modifications, equivalents, improvements, and the like, which are within the spirit and principles of the one or more embodiments in the present application, are therefore intended to be included within the scope of the present application.

Claims

1. An experimental animal heart perfusion apparatus, comprising:

the connecting pipe assembly (10) comprises at least two connecting branch pipes (11) and a connecting main pipe (12), wherein each connecting branch pipe (11) is respectively communicated with one end of the connecting main pipe (12), and a needle head (13) is detachably arranged at the other end of the connecting main pipe (12);

at least two flow velocity adjusting members (20), each flow velocity adjusting member (20) being provided on each connection branch pipe (11) respectively;

a flow control assembly (30) arranged on the connecting main pipe (12), wherein the flow control assembly (30) is used for setting and controlling the quantity of single injection;

the flow control assembly (30) comprises a drip cup (31), a drip speed sensor (32), a monitoring piece (33) and an opening and closing valve (34), wherein the drip speed sensor (32) is arranged on one side of the drip cup (31), the monitoring piece (33) is arranged on the connecting main pipe (12), and the opening and closing valve (34) is arranged at the joint of the monitoring piece (33) and the connecting main pipe (12);

the drop velocity sensor (32) and the on-off valve (34) are electrically connected to the monitor (33), respectively.

2. The experimental animal heart perfusion device according to claim 1, characterized in that the drip rate sensor (32) comprises a transmitting antenna and a receiving antenna, the transmitting antenna transmitting incident microwaves towards the drops in the drip cup (31) at preset time intervals; the receiving antenna receives a reflected wave of the falling reflection of the liquid drop.

3. The experimental animal heart perfusion device according to claim 1, wherein the monitor (33) includes a display screen (331), a control button (332), and a controller (333), and the control button (332), the display screen (331), the drip rate sensor (32), and the on-off valve (34) are all electrically connected to the controller (333).

4. The laboratory animal heart infusion device according to claim 1, wherein said connection main (12) is detachably connected to said flow control assembly (30).

5. The experimental animal heart perfusion device according to claim 1, characterized in that a branch drip cup (111) is provided on each of the connection branches (11), the branch drip cup (111) being provided on the connection branch (11) between the flow rate adjusting member (20) and the connection main tube (12).

6. The laboratory animal heart perfusion device according to claim 1, characterized in that a puncture outfit (112) is provided at the end of each connecting branch tube (11) facing away from the connecting main tube (12).

7. The experimental animal heart perfusion system is characterized by comprising an infusion support (40), an infusion bottle (50) and the experimental animal heart perfusion device according to any one of claims 1-6, wherein the infusion bottle (50) is hung on the upper side of the infusion support (40), and a puncture outfit (112) in the experimental animal heart perfusion device is connected with the infusion bottle (50).

8. The heart infusion system of claim 7, wherein the infusion support (40) comprises a telescopic support (41), a base (42) and an infusion bottle hook (43), the base (42) and the infusion bottle hook (43) are respectively arranged at two ends of the telescopic support (41), and a roller (44) is arranged at one end, which is away from the telescopic support (41), of the base (42).