CN220579241U - Cell centrifugation device for separating cells in vitro - Google Patents
Cell centrifugation device for separating cells in vitro Download PDFInfo
- Publication number
- CN220579241U CN220579241U CN202321902016.2U CN202321902016U CN220579241U CN 220579241 U CN220579241 U CN 220579241U CN 202321902016 U CN202321902016 U CN 202321902016U CN 220579241 U CN220579241 U CN 220579241U
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- China
- Prior art keywords
- groove
- rod
- tray
- rotary
- block
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- 238000005119 centrifugation Methods 0.000 title claims abstract description 16
- 238000000338 in vitro Methods 0.000 title claims description 9
- 238000000926 separation method Methods 0.000 claims description 7
- 230000006378 damage Effects 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 19
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 210000002288 golgi apparatus Anatomy 0.000 description 1
- 210000003712 lysosome Anatomy 0.000 description 1
- 230000001868 lysosomic effect Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 210000001589 microsome Anatomy 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Centrifugal Separators (AREA)
Abstract
The utility model discloses a cell centrifugal device for separating cells from the outside, which comprises a device seat, a tray and a culture dish, wherein a rotary groove is cut at the bottom of the tray, a rotary block is slidably arranged in the rotary groove, one end of the rotary block extends into the rotary groove, the other end of the rotary block extends out of the rotary groove, one end of the rotary block extending out of the rotary groove is fixedly connected with the top of the device seat, side grooves are cut on the inner walls of two sides of the tray, a limit groove is cut on the two sides of the culture dish, a limit rod is slidably arranged in the limit groove, one end of the limit rod extends into the limit groove, the other end of the limit rod extends out of the limit groove, and one end of the limit rod extending out of the limit groove extends into the side groove. The tray is rotationally connected with the device seat through the rotating block, so that cells can be separated by throwing away and rotating the tray when the tray is centrifuged, and the damage to the cells caused by the centrifugation of the machine is avoided.
Description
Technical Field
The utility model relates to the technical field of cell centrifugation devices, in particular to a cell centrifugation device for in-vitro separation of cells.
Background
Cell separation techniques include centrifugation, which is a fundamental means of studying, for example, nuclei, mitochondria, golgi apparatus, lysosomes and microsomes, and various macromolecules, flow cytometry, which is a technique of rapidly and quantitatively analyzing and sorting individual cells, and cell electrophoresis, which is a technique of carrying a net positive or negative charge on the surface of cells at a certain ph value, and which can cause electrophoresis under the action of an applied electric field.
However, when the conventional cell centrifuge is used, there is a certain problem:
the following problems also exist during actual use:
the existing cell separation is basically carried out by a machine, and then when the machine is used for separation, the cell is easy to damage due to the fact that the rotating speed of the machine is too high.
Aiming at the problems, the novel design is carried out on the basis of the original cell centrifugal device.
Disclosure of Invention
The utility model aims to provide a cell centrifugation device for separating cells in vitro, which aims to solve the problem of centrifugation in the background technology.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a cell centrifugal device of external separation of cell, includes device seat, tray and culture dish, the bottom of tray is cut out and is had the rotary tank, and slidable mounting has the rotatory piece in the rotary tank, and the one end of rotatory piece extends to the rotary tank in, and the other end of rotatory piece extends to outside the rotary tank, and the one end that the rotatory piece extends to outside the rotary tank and the top fixed connection of device seat, have all been cut out on the both sides inner wall of tray have the side tank, and both sides of culture dish have all been cut out the spacing groove, slidable mounting has the gag lever post in the spacing groove, and the one end of gag lever post extends to in the spacing groove, and the other end of gag lever post extends to outside the spacing groove in the one end of spacing groove extends to the side tank;
a track groove is cut on the inner wall of the top of the side groove, a track block is slidably mounted in the track groove, one end of the track block extends into the side groove, and the other end of the track block extends into the track groove;
one end of the track block extending into the side groove is fixedly provided with a translation rod, one end of the limit rod extending into the side groove is fixedly connected with one side of the translation rod, one side of the translation rod is cut with a moving groove, and the moving block is slidably arranged in the moving groove;
a square hole is cut on the inner wall of one side of the side groove, a first rotating shaft is rotatably arranged in the square hole, a rotating rod is rotatably arranged on the first rotating shaft, one end of the rotating rod extends into the side groove, the other end of the rotating rod extends out of the side groove, a second rotating shaft is rotatably arranged at one end of the rotating rod extending into the side groove, and the second rotating shaft is fixedly connected with one end of the moving block;
the two sides of the tray are provided with grooves, and clamping rods are slidably arranged in the grooves.
Preferably, the culture dish is located inside the tray, and the side grooves are located on the inner walls of the tops of the two sides of the tray.
By adopting the technical scheme, the culture dish is fixedly connected with the tray through the limiting rod.
Preferably, one side of the track block is fixedly provided with one end of a first spring, and the other end of the first spring is fixedly connected with the inner wall of one side of the track groove.
By adopting the technical scheme, the first spring is used for driving the movable rear track block to reset.
Preferably, the moving block is located at one side of the translation rod away from the limit rod.
By adopting the technical scheme, the moving block drives the translation rod to horizontally move.
Preferably, the clamping rod is of an L-shaped structure.
By adopting the technical scheme, the clamping rod is used for fixing the rotating rod after rotation.
Preferably, one end of a second spring is fixedly arranged at the bottom of the clamping rod, and the other end of the second spring is fixedly connected with the inner wall of the bottom of the channel.
By adopting the technical scheme, the second spring is used for driving the moved clamping rod to reset.
Compared with the prior art, the utility model has the beneficial effects that:
the tray is rotationally connected with the device seat through the rotating block, so that cells can be separated by throwing away the tray to rotate when the tray is centrifuged, and cell damage caused by machine centrifugation is avoided.
Drawings
FIG. 1 is a schematic diagram of the front of the structure of the present utility model;
FIG. 2 is a schematic view of part of the structure A of the present utility model;
FIG. 3 is a schematic view of a portion of the structure B of the present utility model.
In the figure: 1. a device seat; 2. a tray; 3. a rotary groove; 4. a rotating block; 5. a side groove; 6. a limit groove; 7. a limit rod; 8. a track groove; 9. a track block; 10. a translation rod; 11. a moving groove; 12. a moving block; 13. square holes; 14. a rotating lever; 15. a channel; 16. and (5) clamping the rod.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to fig. 1-3, the present utility model provides a technical solution: the utility model provides a cell centrifugation device of external separation of cell, including device seat 1, tray 2 and culture dish, the bottom of tray 2 is excavated there is rotatory groove 3, sliding fit has rotatory piece 4 in rotatory groove 3, the one end of rotatory piece 4 extends to rotatory inslot 3, the other end of rotatory piece 4 extends to outside rotatory groove 3, the one end that rotatory piece 4 extends to outside rotatory groove 3 and the top fixed connection of device seat 1, all open on the both sides inner wall of tray 2 has side groove 5, both sides of culture dish have all been excavated spacing groove 6, sliding fit has gag lever post 7 in spacing groove 6, the one end of gag lever post 7 extends to in the spacing groove 6, the other end of gag lever post 7 extends to outside spacing groove 6, the one end that gag lever post 7 extends to outside spacing groove 6 extends to in the side groove 5.
As shown in fig. 1 to 3, a track groove 8 is cut on the top inner wall of the side groove 5, a track block 9 is slidably installed in the track groove 8, one end of the track block 9 extends into the side groove 5, the other end of the track block 9 extends into the track groove 8, a translation rod 10 is fixedly installed at one end of the track block 9 extending into the side groove 5, one end of a limit rod 7 extending into the side groove 5 is fixedly connected with one side of the translation rod 10, a movable groove 11 is cut on one side of the translation rod 10, and a movable block 12 is slidably installed in the movable groove 11.
As shown in fig. 1-2, a square hole 13 is cut in the inner wall of one side of the side groove 5, a first rotating shaft is rotatably installed in the square hole 13, a rotating rod 14 is rotatably installed in the first rotating shaft, one end of the rotating rod 14 extends into the side groove 5, the other end of the rotating rod 14 extends out of the side groove 5, a second rotating shaft is rotatably installed at one end of the rotating rod 14 extending into the side groove 5, the second rotating shaft is fixedly connected with one end of the moving block 12, a groove 15 is cut in both sides of the tray 2, and a clamping rod 16 is slidably installed in the groove 15.
The working principle of the utility model is as follows: when the centrifugal machine is required, the clamping rod 16 is firstly moved downwards, then the rotating rod 14 is rotated, the rotating rod 14 drives the second rotating shaft to rotate, the second rotating shaft drives the moving block 12 to rotate upwards, the moving block 12 drives the moving rod 10 to horizontally move, the moving rod 10 drives the limiting rod 7 to horizontally move, the limiting rod 7 is retracted into the side groove 5, then the clamping rod 16 is released, the second spring drives the clamping rod 16 to move upwards, the clamping rod 16 clamps the rotating rod 14, then a culture dish is placed in the tray 2, then the clamping rod 16 is moved downwards, the clamping rod 16 is separated from the rotating rod 14, then the first spring drives the track block 9 to horizontally move, the track block 9 drives the moving rod 10 to horizontally move, the moving rod 10 drives the limiting rod 7 to horizontally move, the limiting rod 7 is clamped into the limiting groove 6, and then the tray 2 is rotated, so that the centrifugal machine achieves the purpose.
While embodiments of the present utility model have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations may be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The utility model provides a cell centrifugation device of cell external separation, includes device seat (1), tray (2) and culture dish, its characterized in that:
the bottom of the tray (2) is provided with a rotary groove (3), a rotary block (4) is arranged in the rotary groove (3) in a sliding manner, one end of the rotary block (4) extends into the rotary groove (3), the other end of the rotary block (4) extends out of the rotary groove (3), one end of the rotary block (4) extends out of the rotary groove (3) is fixedly connected with the top of the device seat (1), side grooves (5) are formed in the inner walls of the two sides of the tray (2), limit grooves (6) are formed in the two sides of the culture dish, a limit rod (7) is arranged in the limit grooves (6) in a sliding manner, one end of the limit rod (7) extends into the limit grooves (6), the other end of the limit rod (7) extends out of the limit grooves (6), and one end of the limit rod (7) extends out of the limit grooves (6) into the side grooves (5).
A track groove (8) is cut on the top inner wall of the side groove (5), a track block (9) is slidably installed in the track groove (8), one end of the track block (9) extends into the side groove (5), and the other end of the track block (9) extends into the track groove (8);
one end of the track block (9) extending into the side groove (5) is fixedly provided with a translation rod (10), one end of the limit rod (7) extending into the side groove (5) is fixedly connected with one side of the translation rod (10), one side of the translation rod (10) is provided with a moving groove (11), and a moving block (12) is slidably arranged in the moving groove (11);
a square hole (13) is cut on the inner wall of one side of the side groove (5), a first rotating shaft is rotatably arranged in the square hole (13), a rotating rod (14) is rotatably arranged on the first rotating shaft, one end of the rotating rod (14) extends into the side groove (5), the other end of the rotating rod (14) extends out of the side groove (5), a second rotating shaft is rotatably arranged at one end of the rotating rod (14) extending into the side groove (5), and the second rotating shaft is fixedly connected with one end of the moving block (12);
both sides of the tray (2) are provided with grooves (15), and clamping rods (16) are slidably arranged in the grooves (15).
2. An in vitro cell centrifugation device according to claim 1, wherein: the culture dish is located inside the tray (2), and the side grooves (5) are located on the inner walls of the tops of the two sides of the tray (2).
3. An in vitro cell centrifugation device according to claim 1, wherein: one side of the track block (9) is fixedly provided with one end of a first spring, and the other end of the first spring is fixedly connected with the inner wall of one side of the track groove (8).
4. An in vitro cell centrifugation device according to claim 1, wherein: the moving block (12) is positioned on one side of the translation rod (10) far away from the limiting rod (7).
5. An in vitro cell centrifugation device according to claim 1, wherein: the clamping rod (16) is of an L-shaped structure.
6. An in vitro cell centrifugation device according to claim 1, wherein: one end of a second spring is fixedly arranged at the bottom of the clamping rod (16), and the other end of the second spring is fixedly connected with the inner wall of the bottom of the channel (15).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321902016.2U CN220579241U (en) | 2023-07-19 | 2023-07-19 | Cell centrifugation device for separating cells in vitro |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321902016.2U CN220579241U (en) | 2023-07-19 | 2023-07-19 | Cell centrifugation device for separating cells in vitro |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220579241U true CN220579241U (en) | 2024-03-12 |
Family
ID=90110392
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321902016.2U Active CN220579241U (en) | 2023-07-19 | 2023-07-19 | Cell centrifugation device for separating cells in vitro |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220579241U (en) |
-
2023
- 2023-07-19 CN CN202321902016.2U patent/CN220579241U/en active Active
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