CN220530732U - Simple gravity chromatographic column device - Google Patents
Simple gravity chromatographic column device Download PDFInfo
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- CN220530732U CN220530732U CN202321652158.8U CN202321652158U CN220530732U CN 220530732 U CN220530732 U CN 220530732U CN 202321652158 U CN202321652158 U CN 202321652158U CN 220530732 U CN220530732 U CN 220530732U
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- chromatographic column
- flange
- screen
- column body
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- 230000005484 gravity Effects 0.000 title claims abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 238000004080 punching Methods 0.000 claims abstract description 5
- 238000007789 sealing Methods 0.000 claims description 14
- 229910001220 stainless steel Inorganic materials 0.000 claims description 14
- 239000010935 stainless steel Substances 0.000 claims description 14
- 230000008093 supporting effect Effects 0.000 claims description 14
- 239000004677 Nylon Substances 0.000 claims description 3
- 239000004743 Polypropylene Substances 0.000 claims description 3
- 229920001778 nylon Polymers 0.000 claims description 3
- -1 polypropylene Polymers 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 239000011521 glass Substances 0.000 description 9
- 239000004744 fabric Substances 0.000 description 8
- 238000004587 chromatography analysis Methods 0.000 description 7
- 239000000463 material Substances 0.000 description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 239000004417 polycarbonate Substances 0.000 description 4
- 229920000515 polycarbonate Polymers 0.000 description 4
- 239000000741 silica gel Substances 0.000 description 4
- 229910002027 silica gel Inorganic materials 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 229920002943 EPDM rubber Polymers 0.000 description 2
- 102000003990 Urokinase-type plasminogen activator Human genes 0.000 description 2
- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 description 2
- 230000007797 corrosion Effects 0.000 description 2
- 238000005260 corrosion Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000004811 liquid chromatography Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 229960005356 urokinase Drugs 0.000 description 2
- 239000010963 304 stainless steel Substances 0.000 description 1
- 229920005372 Plexiglas® Polymers 0.000 description 1
- 229910000589 SAE 304 stainless steel Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000012444 downstream purification process Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The utility model provides a simple gravity chromatographic column device, which comprises a chromatographic column body, a screen protection net, a screen plate and a flange column bottom; the chromatographic column body is a cylindrical body, an opening is arranged at the upper end of the chromatographic column body, and a skirt edge is arranged at the opening of the lower end of the chromatographic column body; the screen protection net is of a net structure, the screen plate is of a circular plate, the bottom of the flange column comprises an upper layer and a lower layer, the circle centers of the upper layer and the lower layer are respectively provided with an opening, a valve for controlling liquid to flow out is connected through the openings, and a plurality of punching holes are formed in the plane of the screen plate; the skirt edge, the screen protection net, the screen plate and the flange column bottom of the chromatographic column body are sequentially stacked from top to bottom, the skirt edge and the flange column bottom of the chromatographic column body are fastened together through fasteners, the screen protection net, the screen plate and the screen plate are clamped by the skirt edge of the chromatographic column body and the upper layer of the flange column bottom, and the upper layer of the flange column bottom is abutted to the screen plate. The device is easy to detach, convenient to clean, transport and carry, simple in structure and convenient to operate.
Description
Technical Field
The utility model relates to the technical field of biological separation and purification equipment, in particular to a simple gravity chromatographic column device.
Background
Chromatography, also called chromatography, is a technology discovered and rapidly developed in the beginning of the 20 th century, and has become an extremely important component in the technology of separating and purifying biomacromolecules because of the advantages of high resolution, mild conditions, etc. because it can separate target products from complex mixtures, remove impurities in the target products, replace solutions of the products, etc.
In various downstream purification processes, with the development of technology, whether small-scale development or large-scale production, the liquid chromatography is most widely applied, and chromatography columns matched with the liquid chromatography columns in the market are classified into gravity type, normal pressure, medium pressure, high pressure and ultrahigh pressure according to pressure, and are classified into inorganic glass, organic glass, stainless steel or an organic glass and stainless steel combination according to materials, and the like, and are simultaneously provided with an upper column head, a lower column head, a column temperature control device, a disassembly unit, a moving unit, a column loading system, a sample loading pressurizing system, an on-line monitoring and monitoring system, a collecting or step-by-step collecting system, an automatic control system and the like.
However, for the extraction of large amounts of biological raw materials, the process is simple, the product value is slightly low, and the volume is huge, for example, a large amount of urine and silica gel adsorption materials are needed in urokinase extraction, and the characteristics determine that the production of the urokinase has large and simple requirements and is low in price. However, the currently marketed chromatography equipment is complicated in structure, expensive in price and not exactly matched with the equipment of the process, and the column heads are required to be assembled and disassembled continuously to fill or pump away the filler in production, so that the operation is inconvenient, the labor force is wasted, and in addition, the risks of damaging the column bodies, the column heads and the screen mesh and the screen plate are also existed.
Therefore, there is an urgent need to design a chromatographic apparatus that is structurally simple enough, technically compatible enough, and inexpensive enough.
Disclosure of Invention
The utility model provides a simple gravity chromatographic column device which is used for overcoming the defects of a chromatographic column in the prior art, is easier to disassemble, clean, transport and carry, and has the advantages of simple structure, convenient operation, corrosion resistance, oxidation resistance and difficult breakage.
The technical scheme of the utility model is as follows:
a simple gravity chromatographic column device, which is characterized in that: comprises a chromatographic column body, a screen protection net, a screen plate and a flange column bottom; the chromatographic column body is a cylindrical body, an opening is formed in the upper end of the chromatographic column body, and a skirt edge is arranged at the opening of the lower end of the chromatographic column body; the screen protection net is of a net structure, the screen plate is of a circular plate, the flange column bottom comprises an upper layer and a lower layer, the lower layer is of a circular plate structure with a certain thickness, the upper layer is adhered to the upper surface of the lower layer, the upper layer is of a circular ring plate structure with a certain thickness, the inner diameter of the upper layer is not larger than that of a chromatographic column body, openings are formed in the circle centers of the upper layer and the lower layer, valves for controlling liquid outflow are connected through the openings, and in addition, a plurality of punching holes are uniformly distributed on the plane of the screen plate; the skirt edge of the chromatographic column body, the screen protection net, the screen plate and the flange column bottom are sequentially stacked from top to bottom, and the skirt edge of the chromatographic column body and the flange column bottom are fastened together through fasteners, wherein the screen protection net, the screen plate and the screen plate are clamped by the skirt edge of the chromatographic column body and the upper layer of the flange column bottom, and the upper layer of the flange column bottom is abutted to the screen plate.
Further, the chromatographic column body is made of organic glass, polycarbonate or stainless steel.
Further, the fastener adopts a bolt.
Further, the flange column bottom is made of organic glass, polycarbonate or stainless steel.
Further, threads are arranged in the open holes, and a valve for controlling liquid to flow out can be connected through the threads.
Further, the outer diameter of the lower layer of the flange column bottom is the same as the outer diameter of the skirt edge of the chromatographic column body, and the outer diameter of the upper layer of the flange column bottom is the same as the outer diameter of the lower layer.
Further, the external diameter of the sieve plate is not less than the internal diameter of the upper layer of the flange column bottom, the sieve plate is made of stainless steel, the bottom of the sieve plate is positioned on a plane concentric with the circumference the same as the internal diameter of the upper layer of the flange column bottom, a plurality of supporting legs are further arranged on the plane, and the heights of the supporting legs are the same as the thickness of the upper layer of the flange column bottom.
Further, the screen is made of stainless steel, nylon or polypropylene.
Further, a first annular sealing gasket is arranged between the skirt edge of the chromatographic column body and the screen protection net.
Further, a second annular sealing gasket is arranged between the screen mesh and the screen plate.
The technical scheme provided by some embodiments of the present application has the beneficial effects that at least includes:
through setting up chromatographic column body, screen cloth guard net, screen cloth, sieve, flange post end to stacks the shirt rim of chromatographic column body, screen cloth guard net, screen cloth, sieve, flange post end in proper order, by fastener fastening connection chromatographic column body and flange post end, and screen cloth guard net, screen cloth and sieve are held by the shirt rim of chromatographic column body and the upper strata at flange post end, finally form gravity chromatographic column device, the upper end opening of accessible chromatographic column main part is put into mixed solution, carry out the chromatography to mixed solution through screen cloth guard net, screen cloth and sieve, get rid of impurity in the target product, obtain the target product, this device is dismantled easily, convenient washings, transportation and transport, moreover simple structure, convenient operation.
Drawings
In order to more clearly illustrate the embodiments of the present utility model, the following description will briefly explain the embodiments or the drawings required for the description of the prior art, it being obvious that the drawings in the following description are only some embodiments of the present utility model and that other drawings may be obtained according to these drawings without inventive effort to a person skilled in the art.
FIG. 1 is a schematic diagram showing an exploded structure of a chromatographic column device according to an embodiment of the utility model;
FIG. 2 is a schematic view of a screen guard net according to an embodiment of the present utility model;
FIG. 3 is a schematic view of a screen according to an embodiment of the present utility model;
fig. 4 is a schematic structural view of a screen plate according to an embodiment of the present utility model;
fig. 5 is a schematic structural view of a screen deck support leg according to an embodiment of the present utility model;
FIG. 6 is a schematic diagram of a flange column bottom according to an embodiment of the present utility model;
fig. 7 is a schematic structural view of a first annular gasket according to an embodiment of the present utility model.
The following describes the embodiments of the present utility model in further detail with reference to the drawings.
Detailed Description
In describing embodiments of the present utility model in detail, the drawings showing the structure of the device are not to scale locally for ease of illustration, and the schematic illustrations are merely examples, which should not limit the scope of the utility model. It should be noted that the drawings are in simplified form and are not to scale precisely, but rather are merely intended to facilitate and clearly illustrate the embodiments of the present utility model. It should be noted that the connection mentioned in the present application may be a direct connection or an indirect connection.
As shown in fig. 1-7, a simple gravity chromatographic column device comprises a chromatographic column body 1, a screen protection net 2, a screen 3, a screen plate 4 and a flange column bottom 5; the chromatographic column body 1 is a cylindrical body, an opening is formed in the upper end of the chromatographic column body, and a skirt edge is arranged at the opening of the lower end of the chromatographic column body; the screen protection net 2 is of a net structure, the screen 3 is of a net structure, the screen plate 4 is of a circular plate, the flange column bottom 5 comprises an upper layer 51 and a lower layer 52, the lower layer 52 is of a circular plate structure with a certain thickness, the upper layer 51 is adhered to the upper surface of the lower layer 52, the upper layer 51 is of a circular ring plate structure with a certain thickness, the inner diameter of the upper layer 51 is not larger than that of the chromatographic column body 1, openings 53 are formed in the circle centers of the upper layer 51 and the lower layer 52, valves for controlling liquid to flow out are connected through the openings, in addition, a plurality of punching holes are uniformly distributed on the plane of the screen plate 4, and all the punching holes are uniformly distributed in the same concentric circumference as the inner diameter of the upper layer 51 of the flange column bottom 5; the skirt edge of the chromatographic column body 1, the screen protection net 2, the screen 3, the screen plate 4 and the flange column bottom 5 are sequentially stacked from top to bottom, and the skirt edge of the chromatographic column body 1 and the flange column bottom 5 are fastened together by fasteners, wherein the screen protection net 2, the screen 3 and the screen plate 4 are clamped by the skirt edge of the chromatographic column body 1 and the upper layer 51 of the flange column bottom 5, and the upper layer 51 of the flange column bottom 5 is abutted to the screen plate 4.
In some embodiments, the fastening member may be bolts, and when the fastening member is fastened by bolts, a plurality of through holes may be formed at corresponding positions of the skirt edge of the chromatographic column 1 and the upper layer 51 and the lower layer 52 of the flange column bottom 5, and the fastening member may be fastened by bolts sequentially passing through the through holes, for example, two symmetrical through holes may be formed at the skirt edge of the chromatographic column 1 and the flange column bottom 5, and the fastening member may be fastened by two bolts, and in addition, the outer diameter and the thickness of the skirt edge determine the specification according to the diameter of the column, and the outer diameter of the skirt edge is generally 50-100mm greater than the diameter of the column 1.
In other embodiments, the holes on the skirt are small holes, the number of the holes is 8-64, the holes are uniformly distributed on the skirt, the holes are 10-14mm, and the chromatographic column body 1 and the flange column bottom 5 are connected through bolts, so that the fixing and sealing effects are achieved.
In some embodiments, the chromatography column 1 is made of plexiglas, polycarbonate (PC), stainless steel, or the like. Preferably, the upper end opening of the organic glass is ground, the chromatographic column body 1 is formed by adopting seamless splicing or integrally injection molding, and the organic glass has good corrosion resistance and oxidation resistance and can prolong the service life of the chromatographic column body 1.
In some embodiments, the flange column bottom 5 is made of organic glass, polycarbonate (PC), stainless steel, etc., preferably organic glass, which can also increase the service life of the flange column bottom 5.
In some embodiments, threads are provided in the openings, and a valve for controlling the flow of liquid can be screwed through the threads.
In some embodiments, the outer diameter of the lower layer 52 of the flange column bottom 5 is the same as the outer diameter of the skirt of the chromatographic column 1, the outer diameter of the upper layer 51 is the same as the outer diameter of the lower layer 52, and the inner diameter of the upper layer 51 is slightly smaller than the inner diameter of the chromatographic column 1 or the same as the inner diameter of the chromatographic column 1, so that the appearance of the chromatographic device is neat and the internal structure is practical.
In some embodiments, the screen 3 is circular, and is made of nylon, polypropylene, stainless steel, or other materials that are not easy to corrode, and has the same shape and diameter as the screen plate 4, and in addition, the screen 3 is in a mesh structure, and the diameter of the mesh is smaller than the diameter of the adsorption material such as silica gel, resin particles, etc. filled in the chromatographic column.
In some embodiments, the screen protection net 2 has a meshed circular structure with the same diameter as the screen plate 4, and the material can be 316L stainless steel, 304 stainless steel, etc., and the structure is a meshed structure, and the pore diameter of the structure is larger than that of the screen 3.
In some embodiments, the screen plate 4 is circular, the outer diameter of the screen plate 4 is not less than the inner diameter of the upper layer 51 of the flange column bottom, so that the screen plate can be erected on the upper layer 51 of the flange column bottom 5, a certain gap is formed between the screen plate and the lower layer of the flange column bottom, the screen plate 4 is made of stainless steel, stamping small holes with fixed specifications are uniformly distributed on the plane of the circular structure, and the diameter of the stamping small holes is 6-10mm. The bottom of the sieve plate 4 is located on a plane concentric with the same inner diameter of the upper layer 51 of the flange column bottom 5 and is also provided with a plurality of supporting legs 41, the height of each supporting leg 41 is the same as the thickness of the upper layer 51 of the flange column bottom 5, the supporting legs 41 are made of stainless steel, the supporting legs can be integrally formed with the sieve plate 4, in the embodiment, the supporting legs 41 are cylindrical, the diameter is 6-12mm, the height is the same as the thickness of the upper layer 51 of the flange column bottom 5, the number of the supporting legs is 9, the number of the supporting legs is evenly distributed at the periphery, the number of the supporting legs is evenly distributed at the position close to the circle center is 3, and a certain supporting effect is achieved between the sieve plate 4 and the lower layer 52 of the flange column bottom 5, so that the supporting legs are better separated, and a buffer space is reserved for a solution after chromatography.
In some embodiments, a first annular gasket 8 is further provided between the skirt of the chromatographic column body 1 and the screen guard 2. The first annular gasket 8 is preferably made of silica gel, ethylene propylene diene monomer, tetrafluoro or the like, and the inner diameter and the outer diameter of the first annular gasket 8 are the same as those of the upper layer 51 of the flange column bottom 5.
The device further comprises a second annular sealing gasket 9, wherein the second annular sealing gasket 9 is arranged between the screen 3 and the upper layer 51 of the flange column bottom 5, the second annular sealing gasket 9 is also preferably made of silica gel, ethylene propylene diene monomer, tetrafluoro and the like, and the inner diameter and the outer diameter of the second annular sealing gasket 9 are the same as those of the upper layer 51 of the flange column bottom 5.
It should be noted that, holes are uniformly formed on the first annular sealing pad 8 and the second annular sealing pad 9, and the fasteners can pass through the holes, so that the first annular sealing pad 8 and the second annular sealing pad 9 can make the chromatographic column body 1, the flange column bottom 5, the sieve plate 4, the screen 3 and the screen protection net 2 be connected more tightly, thereby playing a good sealing role and preventing the solution from overflowing.
In addition, the device is easy to detach, convenient to clean, transport and carry, simple in structure and convenient to operate.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present utility model. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the utility model. Thus, the present utility model is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (8)
1. A simple gravity chromatographic column device, which is characterized in that: comprises a chromatographic column body, a screen protection net, a screen plate and a flange column bottom; the chromatographic column body is a cylindrical body, an opening is formed in the upper end of the chromatographic column body, and a skirt edge is arranged at the opening of the lower end of the chromatographic column body; the screen protection net is of a net structure, the screen plate is of a circular plate, the flange column bottom comprises an upper layer and a lower layer, the lower layer is of a circular plate structure with a certain thickness, the upper layer is of a circular ring plate structure with a certain thickness, the upper layer is adhered to the upper surface of the lower layer, the inner diameter of the upper layer is not larger than that of a chromatographic column body, openings are formed in the circle centers of the upper layer and the lower layer, valves for controlling liquid outflow are connected through the openings, and in addition, a plurality of punching holes are uniformly distributed on the plane of the screen plate; the skirt edge of the chromatographic column body, the screen protection net, the screen plate and the flange column bottom are sequentially stacked from top to bottom, and the skirt edge of the chromatographic column body and the flange column bottom are fastened together through fasteners, wherein the screen protection net, the screen plate and the screen plate are clamped by the skirt edge of the chromatographic column body and the upper layer of the flange column bottom, and the upper layer of the flange column bottom is abutted to the screen plate.
2. The apparatus of claim 1, wherein: the fastener adopts a bolt.
3. The apparatus of claim 1, wherein: the open hole is internally provided with threads, and the valve for controlling the outflow of liquid can be connected through the threads.
4. The apparatus of claim 1, wherein: the outer diameter of the lower layer of the flange column bottom is the same as the outer diameter of the skirt edge of the chromatographic column body, and the outer diameter of the upper layer of the flange column bottom is the same as the outer diameter of the lower layer.
5. The apparatus of claim 1, wherein: the external diameter of sieve is not less than the internal diameter of flange post bottom upper strata, the sieve adopts stainless steel to make, the bottom of sieve still is provided with a plurality of supporting legs on the plane of the concentric circumference that is located the same with the upper strata internal diameter at flange post bottom, the height of supporting leg is the same with the thickness at flange post bottom upper strata.
6. The apparatus of claim 1, wherein: the screen is made of any one of stainless steel, nylon or polypropylene.
7. The apparatus of claim 1, wherein: and a first annular sealing gasket is further arranged between the skirt edge of the chromatographic column body and the screen protection net.
8. The apparatus of claim 1, wherein: and a second annular sealing gasket is further arranged between the screen mesh and the screen plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321652158.8U CN220530732U (en) | 2023-06-28 | 2023-06-28 | Simple gravity chromatographic column device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321652158.8U CN220530732U (en) | 2023-06-28 | 2023-06-28 | Simple gravity chromatographic column device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220530732U true CN220530732U (en) | 2024-02-27 |
Family
ID=89967140
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321652158.8U Active CN220530732U (en) | 2023-06-28 | 2023-06-28 | Simple gravity chromatographic column device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220530732U (en) |
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2023
- 2023-06-28 CN CN202321652158.8U patent/CN220530732U/en active Active
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