CN220519043U - Extracellular vesicle extraction kit with refrigeration function - Google Patents
Extracellular vesicle extraction kit with refrigeration function Download PDFInfo
- Publication number
- CN220519043U CN220519043U CN202321808856.2U CN202321808856U CN220519043U CN 220519043 U CN220519043 U CN 220519043U CN 202321808856 U CN202321808856 U CN 202321808856U CN 220519043 U CN220519043 U CN 220519043U
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- China
- Prior art keywords
- box body
- tube rack
- outer box
- test
- extracellular vesicle
- Prior art date
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- 238000000605 extraction Methods 0.000 title claims abstract description 20
- 238000005057 refrigeration Methods 0.000 title claims description 17
- 239000004065 semiconductor Substances 0.000 claims abstract description 27
- 238000012360 testing method Methods 0.000 claims abstract description 19
- 238000004321 preservation Methods 0.000 claims abstract description 8
- 229920001971 elastomer Polymers 0.000 claims description 10
- 239000002826 coolant Substances 0.000 claims description 5
- 229920002635 polyurethane Polymers 0.000 claims description 5
- 239000004814 polyurethane Substances 0.000 claims description 5
- 238000002955 isolation Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 230000005855 radiation Effects 0.000 claims 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 6
- 239000000110 cooling liquid Substances 0.000 abstract description 6
- 238000001816 cooling Methods 0.000 abstract description 5
- 230000006866 deterioration Effects 0.000 abstract description 4
- 230000002035 prolonged effect Effects 0.000 abstract description 4
- 230000003647 oxidation Effects 0.000 abstract description 2
- 238000007254 oxidation reaction Methods 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 5
- 239000010410 layer Substances 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- 230000000694 effects Effects 0.000 description 3
- 238000009413 insulation Methods 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 2
- 239000011229 interlayer Substances 0.000 description 2
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 2
- 230000033115 angiogenesis Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000023402 cell communication Effects 0.000 description 1
- 230000012292 cell migration Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000004565 tumor cell growth Effects 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The utility model discloses an extracellular vesicle extraction kit with a refrigerating function, which comprises an outer box body, an inner box body, a test tube rack and a semiconductor refrigerating piece, wherein the inner box body, the test tube rack and the semiconductor refrigerating piece are arranged in the outer box body, the semiconductor refrigerating piece is arranged at the lowest part in the outer box body, the inner box body is arranged at the top of the semiconductor refrigerating piece, the test tube rack is clamped at the top of the inner box body, a jack is formed in the surface of the test tube rack, a limiting sleeve is fixedly connected to the bottom of the test tube rack, and the limiting sleeve is arranged right below the jack. According to the utility model, the inner box body, the test tube rack and the semiconductor refrigerating piece are arranged in the outer box body, the reagent tube is inserted into the test tube rack, the semiconductor refrigerating piece is used for refrigerating the cooling liquid in the inner box body, the low-temperature cooling liquid is used for absorbing the heat of the reagent tube in the limit sleeve, the cooling of the extracting solution is realized, and the oxidation and deterioration rate of the extracting solution can be reduced after the extracting solution is cooled, so that the preservation time is prolonged.
Description
Technical Field
The utility model relates to the technical field of biological tissue extraction kits, in particular to an extracellular vesicle extraction kit with a refrigerating function.
Background
Extracellular vesicles are membranous vesicles released from cells to extracellular matrixes, have diameters ranging from 40nm to 1000nm, have roles equivalent to vehicles, participate in processes such as cell communication, cell migration, angiogenesis and tumor cell growth, are widely present in various body fluids and cell supernatants, and stably carry some important signal molecules. The study of vesicle-related functions has become a research hotspot and is expected to play a role in the early diagnosis of various diseases.
When the existing biological tissue extraction kits are used, the cooling structure is not arranged on the biological tissue extraction kits, so that the extracting solution cannot be cooled, and the extracting solution is easy to deteriorate rapidly after being stored at a higher temperature for a long time, so that the preservation time of the extracting solution is not facilitated to be prolonged.
For example, the publication number is a PBMC cell extraction kit disclosed, this patent is through setting up vacuum chuck to make rubber sucking disc and the terminal surface of lid cooperate, when external force presses, rubber sucking disc and the air in the arc recess are extruded, form the vacuum, under the effect of atmospheric pressure, make the lid firmly be fixed in the box body top, and then improved the leakproofness of this kit, form a sterile environment and be favorable to the preservation of cell, guaranteed the activity of the PBMC cell of drawing simultaneously, can effectively restrain the degradation of extract.
However, this patent only can create a vacuum sealed environment inside the kit, and although external contaminants are isolated to some extent, the extracted cells are stuffy inside the kit, and the heat dissipated by the cells themselves is accumulated inside the kit, so that the deterioration rate is easily increased, and the preservation time is not facilitated to be prolonged.
Therefore, it is necessary to invent an extracellular vesicle extraction kit having a refrigerating function to solve the above-mentioned problems.
Disclosure of Invention
The utility model aims to provide an extracellular vesicle extraction kit with a refrigerating function, which solves the problems that when the existing biological tissue extraction kit in the prior art is used, a cooling structure is not arranged on the existing biological tissue extraction kit, the extracting solution cannot be cooled, the extracting solution is easy to deteriorate rapidly after being stored at a higher temperature for a long time, and the preservation time of the extracting solution is not easy to prolong.
In order to achieve the above object, the present utility model provides the following technical solutions: the utility model provides an extracellular vesicle draws kit with cold-stored function, includes outer box body, interior box body, test-tube rack and semiconductor refrigeration piece all set up in outer box body inside, semiconductor refrigeration piece is installed in outer box body inside below, interior box body is installed in semiconductor refrigeration piece top, test-tube rack joint is in interior box body top, the jack has been seted up on the test-tube rack surface, test-tube rack bottom fixedly connected with limit sleeve, limit sleeve sets up under the jack, limit sleeve sets up in interior box body inside, interior box body inside is filled with the coolant liquid.
Preferably, the joint department of test-tube rack and interior box body is provided with rubber seal, rubber seal is fixed cup joints in interior box body top edge, blocks off the gap between test-tube rack and the interior box body through rubber seal, avoids the coolant liquid to leak out from interior box body.
Preferably, a power interface is provided on one side of the outer case, the power interface is electrically connected with the semiconductor refrigeration sheet, the external power line is connected with the power interface to supply power to the semiconductor refrigeration sheet, and one side of the semiconductor refrigeration sheet absorbs heat after being electrified and the other side emits heat.
Preferably, the handle is No. one at both ends of test-tube rack top, interior box body, test-tube rack, spacing sleeve and handle are made by medical stainless steel material, can draw the test-tube rack down from interior box body top through handle No. one.
Preferably, the four corners of outer box body bottom all fixedly connected with pad foot, the radiating groove has been seted up in the middle of the outer box body bottom, radiating groove internally mounted has the isolation net, can cushion the certain distance with outer box body bottom through the pad foot, makes things convenient for the semiconductor refrigeration piece to dispel the heat from the radiating groove.
Preferably, the top of the outer box body is hinged with a top cover, the top of the top cover is hinged with a second handle, and the whole device can be lifted through the second handle.
Preferably, the interlayer is arranged in the outer box body and the top cover, the polyurethane heat insulation layer is filled in the interlayer, and the polyurethane heat insulation layer is arranged to be favorable for improving the overall heat insulation performance of the device.
Preferably, the outer box body surface fixedly connected with padlock mounting, the padlock moving part is installed to top cap one side, the padlock moving part locks with the padlock mounting mutually, through the cooperation of padlock moving part and padlock mounting, conveniently is in the same place top cap and outer box body fixed.
In the technical scheme, the utility model has the technical effects and advantages that:
through set up interior box body, test-tube rack and semiconductor refrigeration piece in outer box body, insert the reagent pipe behind the test-tube rack, refrigerate the coolant liquid in the interior box body by the semiconductor refrigeration piece, absorb the heat of spacing sleeve inside reagent pipe through low temperature coolant liquid, realize the cooling to the extract, can reduce the rate of oxidative deterioration after the extract is cooled down to extension save time.
Drawings
FIG. 1 is a schematic view of the overall structure of a first view angle according to the present utility model;
FIG. 2 is a schematic view of the overall structure of a second view angle according to the present utility model;
FIG. 3 is a schematic view of the overall structure of the top cover of the present utility model when the top cover is opened;
FIG. 4 is a cross-sectional view of the structure of the present utility model;
fig. 5 is a schematic structural view of the inner case and the test tube rack of the present utility model.
Reference numerals illustrate:
1. an outer case; 2. an inner case; 3. a test tube rack; 4. a semiconductor refrigeration sheet; 5. a jack; 6. a limit sleeve; 7. a rubber seal ring; 8. a power interface; 9. a first handle; 10. foot pads; 11. an isolation net; 12. a top cover; 13. a second handle; 14. a polyurethane heat-insulating layer; 15. a snap lock fastener; 16. the movable part is locked.
Detailed Description
In order to make the technical scheme of the present utility model better understood by those skilled in the art, the present utility model will be further described in detail with reference to the accompanying drawings.
The utility model provides an extracellular vesicle extraction kit with a refrigerating function as shown in figures 1-5, which comprises an outer box body 1, an inner box body 2, a test tube rack 3 and a semiconductor refrigerating piece 4, wherein the inner box body 2, the test tube rack 3 and the semiconductor refrigerating piece 4 are arranged in the outer box body 1, the semiconductor refrigerating piece 4 is arranged at the lowest part in the outer box body 1, the inner box body 2 is arranged at the top of the semiconductor refrigerating piece 4, the test tube rack 3 is clamped at the top of the inner box body 2, an inserting hole 5 is formed in the surface of the test tube rack 3, a limit sleeve 6 is fixedly connected to the bottom of the test tube rack 3, the limit sleeve 6 is arranged under the inserting hole 5, the limit sleeve 6 is arranged in the inner box body 2, and cooling liquid is filled in the inner box body 2.
In one aspect of this embodiment, the joint department of test-tube rack 3 and interior box body 2 is provided with rubber seal 7, rubber seal 7 fixedly cup joints in interior box body 2 top edge, power source 8 has been seted up to outer box body 1 one side, power source 8 and semiconductor refrigeration piece 4 electric connection, the equal fixedly connected with handle 9 in test-tube rack 3 top both ends, interior box body 2, test-tube rack 3, spacing sleeve 6 and handle 9 are made by medical stainless steel material, outer box body 1 bottom four corners all fixedly connected with pad foot 10, the radiating groove has been seted up in the middle of the outer box body 1 bottom, radiating groove internally mounted has isolation net 11, outer box body 1 top articulates and is provided with top cap 12, top cap 12 top articulates is provided with No. two handles 13, the intermediate layer has all been seted up inside and the top cap 12 of outer box body 1, the intermediate layer is inside to be filled with polyurethane heat preservation 14, outer box body 1 surface fixedly connected with buckle fixed 15, buckle movable piece 16 is installed to top cap 12 one side, buckle movable piece 16 looks buckle fixed 15.
The working principle of the utility model is as follows:
referring to fig. 1-5 of the specification, when the utility model is used, a certain amount of cooling liquid is firstly injected into the inner box body 2, then the test tube rack 3 is clamped at the top of the inner box body 2, so that the limit sleeve 6 at the bottom of the test tube rack 3 is immersed in the cooling liquid, a reagent tube filled with extracellular vesicle extracting liquid is inserted into the limit sleeve 6 through the insertion hole 5, then the top cover 12 is covered at the top of the outer box body 1, the top cover 12 and the outer box body 1 are fixed together by means of the buckling connection of the buckling movable piece 16 and the buckling fixed piece 15, finally an external power line is connected with the power interface 8, the semiconductor refrigerating piece 4 begins to absorb heat at the side which is attached to the inner box body 2 after being electrified, and releases heat from the other side outwards, after the temperature of the cooling liquid in the inner box body 2 is reduced, the heat from the reagent tube in the limit sleeve 6 is absorbed, the cooling of the extracting liquid is realized, and the rate of oxidation deterioration can be reduced after the extracting liquid is cooled, so that the preservation time is prolonged.
Claims (8)
1. The utility model provides an extracellular vesicle draws kit with cold-stored function, includes outer box body (1), interior box body (2), test-tube rack (3) and semiconductor refrigeration piece (4), its characterized in that: interior box body (2), test-tube rack (3) and semiconductor refrigeration piece (4) all set up inside outer box body (1), semiconductor refrigeration piece (4) are installed in the inside below of outer box body (1), interior box body (2) are installed in semiconductor refrigeration piece (4) top, jack (5) have been seted up on test-tube rack (3) surface in interior box body (2) top, jack (5) are seted up to test-tube rack (3) bottom fixedly connected with spacing sleeve (6), spacing sleeve (6) set up under jack (5), spacing sleeve (6) set up inside interior box body (2), interior box body (2) intussuseption is filled with the coolant.
2. The extracellular vesicle extraction kit with a refrigerating function according to claim 1, wherein: the joint department of test-tube rack (3) and interior box body (2) is provided with rubber seal (7), rubber seal (7) are fixed cup joints in interior box body (2) top edge.
3. The extracellular vesicle extraction kit with a refrigerating function according to claim 1, wherein: a power interface (8) is arranged on one side of the outer box body (1), and the power interface (8) is electrically connected with the semiconductor refrigerating sheet (4).
4. The extracellular vesicle extraction kit with a refrigerating function according to claim 1, wherein: the test tube rack is characterized in that a first handle (9) is fixedly connected to two ends of the top of the test tube rack (3), and the inner box body (2), the test tube rack (3), the limiting sleeve (6) and the first handle (9) are made of medical stainless steel materials.
5. The extracellular vesicle extraction kit with a refrigerating function according to claim 1, wherein: the novel heat radiation box is characterized in that the four corners of the bottom of the outer box body (1) are fixedly connected with pad feet (10), a heat radiation groove is formed in the middle of the bottom of the outer box body (1), and an isolation net (11) is arranged in the heat radiation groove.
6. The extracellular vesicle extraction kit with a refrigerating function according to claim 1, wherein: the top of the outer box body (1) is hinged with a top cover (12), and the top of the top cover (12) is hinged with a second handle (13).
7. The extracellular vesicle extraction kit with a refrigerating function according to claim 6, wherein: the inside of outer box body (1) and top cap (12) are inside to have all seted up the intermediate layer, the inside polyurethane heat preservation (14) that fills of intermediate layer.
8. The extracellular vesicle extraction kit with a refrigerating function according to claim 6, wherein: the outer box body (1) surface fixedly connected with padlock mounting (15), padlock moving part (16) are installed to top cap (12) one side, padlock moving part (16) and padlock mounting (15) looks lock.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321808856.2U CN220519043U (en) | 2023-07-11 | 2023-07-11 | Extracellular vesicle extraction kit with refrigeration function |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321808856.2U CN220519043U (en) | 2023-07-11 | 2023-07-11 | Extracellular vesicle extraction kit with refrigeration function |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220519043U true CN220519043U (en) | 2024-02-23 |
Family
ID=89929561
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321808856.2U Active CN220519043U (en) | 2023-07-11 | 2023-07-11 | Extracellular vesicle extraction kit with refrigeration function |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220519043U (en) |
-
2023
- 2023-07-11 CN CN202321808856.2U patent/CN220519043U/en active Active
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| GR01 | Patent grant | ||
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