CN113120390A - Method for storing and transporting cells at normal temperature - Google Patents
Method for storing and transporting cells at normal temperature Download PDFInfo
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- CN113120390A CN113120390A CN202110516525.0A CN202110516525A CN113120390A CN 113120390 A CN113120390 A CN 113120390A CN 202110516525 A CN202110516525 A CN 202110516525A CN 113120390 A CN113120390 A CN 113120390A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
- B65D25/10—Devices to locate articles in containers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D53/00—Sealing or packing elements; Sealings formed by liquid or plastics material
- B65D53/02—Collars or rings
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/02—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage
- B65D81/05—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage maintaining contents at spaced relation from package walls, or from other contents
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
- B65D81/20—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient under vacuum or superatmospheric pressure, or in a special atmosphere, e.g. of inert gas
- B65D81/2007—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient under vacuum or superatmospheric pressure, or in a special atmosphere, e.g. of inert gas under vacuum
- B65D81/2038—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient under vacuum or superatmospheric pressure, or in a special atmosphere, e.g. of inert gas under vacuum with means for establishing or improving vacuum
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/38—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation
- B65D81/3813—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation rigid container being in the form of a box, tray or like container
- B65D81/3823—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation rigid container being in the form of a box, tray or like container formed of different materials, e.g. laminated or foam filling between walls
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Abstract
The invention discloses a method for storing and transporting cells at normal temperature, which is used for integrally disinfecting and transporting equipment inside and outside the cell. The method for storing and transporting the cells at the normal temperature comprises the steps of carrying out integral internal and external disinfection treatment on a cell storage and transportation device, carrying out integral internal and external disinfection treatment on a glass tube, introducing cell storage and transportation liquid into the glass tube, putting the stored and transported cells into the cell storage and transportation liquid in the glass tube, putting the glass tube filled with the cells and the cell storage and transportation liquid into a placing frame in a fixing frame, protecting the stored and transported cells by using the cell storage and transportation liquid, packaging the cells by using the glass tube, avoiding the problem of cell extrusion caused by using a storage and transportation bag, further enhancing the safety protection of the stored and transported cells, realizing integral heat insulation of the interior of a storage and transportation box by using a heat insulation sleeve I and a heat insulation sleeve II, and carrying out double temperature insulation on the glass tube by matching with a U-shaped tube and a heat insulation block, and further effectively ensuring the cells to be.
Description
Technical Field
The invention relates to the technical field of cell storage and transportation, in particular to a method for storing and transporting cells at normal temperature.
Background
Cell therapy is a new disease treatment technology which is emerging in recent years, and means that certain cells with specific functions are obtained by a bioengineering method and/or are treated by in vitro amplification, special culture and the like, so that the cells have treatment effects of enhancing immunity, killing pathogens and tumor cells, promoting regeneration of tissues and organs, recovering organisms and the like, and the purpose of treating diseases is achieved. Whether the cells are used for cell therapy and other processes after being obtained and/or amplified in vitro, the cold chain transportation of the cells becomes an intermediate key step, particularly in the remote transportation of the cells, the advantages and disadvantages of a transportation tool and a transportation method are directly related to the activity of the cells, and finally the future use of scientific research on the cells or cell therapy is influenced.
In the scheme, cell menstruum is adjusted to be butted with laboratory experiment/production and clinical application, and cells needing to be stored and transported are placed in cell storage and transportation liquid to realize storage and transportation at normal temperature or low temperature; however, normal saline, compound normal saline, lactated ringer's solution or compound electrolyte injection is used as a known cell basic solvent, but the technical point of the proposal is that human serum albumin with the mass fraction of 5-10% is only added into the cell basic solvent, and the method can not achieve a good solution effect on the normal temperature cell storage and transportation of the technical problem, so that the method for storing and transporting the cells at the normal temperature has a certain positive significance.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a method for storing and transporting cells at normal temperature, which solves the problem that the cells are easily damaged when being stored and transported at the normal temperature.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: a method for storing and transporting cells at normal temperature comprises the following steps:
step one, carrying out integral internal and external disinfection treatment on cell storage and transportation equipment;
step two, carrying out integral internal and external disinfection treatment on the glass tube, then introducing cell storage and transportation liquid into the glass tube, putting the stored and transported cells into the cell storage and transportation liquid in the glass tube, blocking a port of the glass tube by using a block, and putting the glass tube filled with the cells and the cell storage and transportation liquid into a placing rack in a fixing frame;
step three, the surface of the glass tube is in contact with a sponge cushion on the inner wall of the U-shaped tube, the bottom end of the glass tube is in contact with an arc-shaped groove on the top of the rubber seat, a threaded block is used for being in threaded connection with the inside of the placing frame, a heat insulation block at the bottom of the threaded block presses down a blocking block at the end port of the glass tube, downward force is applied to the rubber seat by the bottom end of the glass tube, a sliding block is arranged at the bottom of the rubber seat and generates downward pressure, a spring is stressed to shrink, the whole glass tube moves downward in the U-shaped tube until the threaded block is completely in threaded connection with the inside of the placing frame, and the whole glass tube enters the inside;
fourthly, sealing and installing the protection plate and the fixing frame, then sealing the sealing part inside the protection plate and the protection plate, and vacuumizing the space between the protection plate and the fixing frame through a vacuum exhaust pipe;
and fifthly, putting the fixing frame into the storage and transportation box, enabling the concave parts on two sides of the fixing frame and the convex parts on the front and back surfaces of the fixing frame to be respectively in sliding connection with the limiting blocks on the periphery of the inner wall of the first heat insulation sleeve, enabling the middle part of the surface of the fixing frame to be in interference fit connection with the inside of the rubber positioning frame in the first heat insulation sleeve, completing limiting installation of the fixing frame in the storage and transportation box, finally covering the movable cover, sealing the bottom of the movable cover and the top of the storage and transportation box through a sealing ring, finally vacuumizing the inside of the storage and transportation box through a vacuum exhaust pipe on the top of the movable cover, transporting the storage and transportation box, and completing.
Preferably, the cell storage and transportation liquid is a compound liquid of normal saline, ringer's solution and compound electrolyte injection.
Preferably, the cell storage and transportation equipment comprises a storage and transportation box, a movable cover and glass tubes, wherein the movable cover is hinged to one side of the top of the storage and transportation box through hinges, sponge sleeves are arranged on the two sides of the movable cover and the storage and transportation box, a placement mechanism is arranged in the storage and transportation box, a plurality of glass tubes are arranged in the placement mechanism, a heat insulation mechanism and a protection mechanism are arranged in the storage and transportation box respectively, and the protection mechanism is located in the heat insulation mechanism.
Preferably, the heat insulation mechanism comprises a first heat insulation sleeve, a second heat insulation sleeve and a rubber pad, the first heat insulation sleeve is arranged inside the storage and transportation box, the surface of the first heat insulation sleeve is fixedly connected with the inner wall of the storage and transportation box, the rubber pad is arranged on the periphery of the inner wall of the first heat insulation sleeve, the limiting blocks are arranged on the periphery of the inner wall of the rubber pad, the rubber positioning frame is arranged in the middle of the inside of the rubber pad, the second heat insulation sleeve is arranged inside the movable cover, and the surface of the second heat insulation sleeve is fixedly connected with the inner wall of the movable cover.
Preferably, protection machanism includes mount, guard plate, sealing, the concave part has all been seted up to the both sides of mount, and the mount openly all is provided with the convex part with the both sides at the back, concave part and convex part respectively with rubber pad inner wall stopper sliding connection all around, and be interference fit between the surface of mount and the inside of rubber locating rack, the top of mount is provided with the guard plate, and all hinges through the hinge around the guard plate is inside has the sealing, form the confined space between the inside of guard plate, sealing and mount, the inside symmetry that placement mechanism is located the mount is provided with four.
Preferably, the placing mechanism comprises a placing rack, a U-shaped pipe, a sponge cushion, a sliding block, a rubber seat, a spring, a threaded block and a heat insulation block, the placing rack is arranged inside the fixing rack, the U-shaped pipe is arranged on two sides inside the placing rack, the inner wall of the U-shaped pipe is provided with the sponge cushion, the sliding block is slidably connected inside the U-shaped pipe, the rubber seat is arranged at the top of the sliding block, an arc-shaped groove is formed in the top of the rubber seat, the glass pipe is arranged inside the U-shaped pipe, the bottom end of the glass pipe is in contact with the inner wall of the arc-shaped groove, the threaded block is connected inside the fixing rack in a threaded mode, and the.
Preferably, the top end of the U-shaped pipe is provided with a blocking block, the top of the blocking block is in contact with the bottom of the heat insulation block, the bottom of the inner wall of the U-shaped pipe is provided with a spring, and the top end of the spring is fixedly connected with the bottom of the sliding block.
Preferably, the inside of movable cover and guard plate all is provided with the vacuum exhaust tube, be provided with the sealing washer between the bottom of movable cover and the top of storage case, the inner chamber has all been seted up to the inside of adiabatic cover one, adiabatic cover two, U type pipe, thermal-insulated piece, and the inside packing of inner chamber has the refrigerant.
Preferably, the use method of the cell storage and transportation equipment is as follows:
carrying out integral internal and external sterilization treatment on the glass tube, then introducing cell storage and transportation liquid into the glass tube, putting the stored and transported cells into the cell storage and transportation liquid in the glass tube, blocking a port of the glass tube by using a block, and putting the glass tube filled with the cells and the cell storage and transportation liquid into a placing rack in a fixing frame;
the surface of the glass tube is contacted with a sponge cushion on the inner wall of the U-shaped tube, the bottom end of the glass tube is contacted with an arc-shaped groove on the top of the rubber seat, a thread block is in threaded connection with the inside of the placing frame, a heat insulation block at the bottom of the thread block presses a block at the end port of the glass tube down, downward force is applied to the rubber seat by the bottom end of the glass tube, a sliding block is arranged at the bottom of the rubber seat, the sliding block generates downward pressure, a spring is stressed to contract, the whole glass tube moves downward in the U-shaped tube until the thread block is completely in threaded connection with the inside of the placing frame, and the whole glass tube enters the inside of the U;
sealing and installing the protection plate and the fixing frame, then sealing the sealing part inside the protection plate and the protection plate, and vacuumizing the space between the protection plate and the fixing frame through a vacuum exhaust pipe;
the fixed frame is placed in the storage and transportation box, the concave parts on two sides of the fixed frame and the convex parts on the front and back surfaces of the fixed frame are respectively in sliding connection with the limiting blocks on the periphery of the inner wall of the heat insulation sleeve, meanwhile, the middle part of the surface of the fixed frame is in interference fit connection with the inside of the rubber positioning frame in the heat insulation sleeve, the fixed frame is limited and installed in the storage and transportation box, finally, the movable cover is covered, the bottom of the movable cover and the top of the storage and transportation box are sealed through the sealing ring, finally, the inside of the storage and transportation box is vacuumized through the vacuum exhaust tube on the top of the movable cover, the storage and transportation box is transported.
(III) advantageous effects
The invention provides a method for storing and transporting cells at normal temperature. Compared with the prior art, the method has the following beneficial effects:
the method for storing and transporting the cells at the normal temperature comprises the steps of carrying out integral internal and external disinfection treatment on a cell storage and transportation device, carrying out integral internal and external disinfection treatment on a glass tube, then introducing cell storage and transportation liquid into the glass tube, putting the stored and transported cells into the cell storage and transportation liquid in the glass tube, blocking a port of the glass tube by using a blocking block, putting the glass tube filled with the cells and the cell storage and transportation liquid into a placing frame in a fixing frame, protecting the stored and transported cells by using the cell storage and transportation liquid, and packaging the cells by using the glass tube, so that the problem of cell extrusion caused by using a storage and transportation bag can be avoided, and further the safety protection on the stored and transported cells is enhanced;
the surface of the glass tube is contacted with a sponge cushion on the inner wall of the U-shaped tube, the bottom end of the glass tube is contacted with an arc-shaped groove on the top of a rubber seat, a thread block is in threaded connection with the inside of the placing frame, a heat insulation block at the bottom of the thread block presses a block at the end opening of the glass tube, downward force is applied to the rubber seat by the bottom end of the glass tube, a sliding block is arranged at the bottom of the rubber seat and generates downward pressure, a spring is stressed to contract, the whole glass tube moves downwards in the U-shaped tube until the thread block is completely in threaded connection with the inside of the placing frame, the whole glass tube enters the inside of the U-shaped tube, the U-shaped tube and the heat insulation block are used for coating the glass tube, and the inside of the U-shaped tube and the heat insulation block are filled with refrigerants, so that the temperature change of the glass tube in the storage and, then, the sealing part inside the protection plate is sealed with the protection plate, and the protection plate and the fixing frame are vacuumized through the vacuum exhaust tube, so that the vacuum state inside the fixing frame is ensured, and the influence of gas circulation on the cell activity inside the glass tube can be avoided;
by placing the fixing frame into the storage and transportation box, the concave parts at two sides of the fixing frame and the convex parts at the front and the back are respectively connected with the limit blocks at the periphery of the inner wall of the heat insulation sleeve in a sliding way, meanwhile, the middle part of the surface of the fixed frame is connected with the inside of the rubber positioning frame in the heat-insulating sleeve I in an interference fit manner, the fixed frame is limited and installed in the storage and transportation box, finally, the movable cover is covered, the bottom of the movable cover and the top of the storage and transportation box are sealed through a sealing ring, and finally, the inside of the storage and transportation box is vacuumized through a vacuum exhaust pipe at the top of the movable cover, transporting the storage and transportation box to finish the storage and transportation of cells at normal temperature, realizing the integral heat insulation of the interior of the storage and transportation box by utilizing the first heat insulation sleeve and the second heat insulation sleeve, and the glass tube is insulated at double temperatures by matching with the U-shaped tube and the heat insulation block, so that the storage and the transportation of cells under the normal temperature condition are effectively ensured.
Drawings
FIG. 1 is a schematic view of the structure of the cell storage and transportation apparatus according to the present invention;
FIG. 2 is a top view of a storage and transport container, protective mechanism, and insulating jacket arrangement of the present invention;
FIG. 3 is a schematic view of a shield plate structure according to the present invention;
FIG. 4 is a cross-sectional view of the placement mechanism configuration of the present invention;
FIG. 5 is an enlarged view of the structure of FIG. 4 at A according to the present invention;
FIG. 6 is a schematic view of the movable cover and the heat-insulating sleeve of the present invention.
In the figure, 1, a placing mechanism; 11. placing a rack; 12. a U-shaped pipe; 13. a sponge cushion; 14. a slider; 15. a rubber seat; 16. a spring; 17. a thread block; 18. a heat insulation block; 19. blocking; 2. a heat insulating mechanism; 21. a first heat insulation sleeve; 22. a second heat insulation sleeve; 23. a rubber pad; 24. a limiting block; 25. a rubber positioning frame; 3. a protection mechanism; 31. a fixed mount; 32. a protection plate; 33. a sealing part; 4. a storage and transport box; 5. a movable cover; 6. a glass tube; 7. a sponge sleeve; 8. a vacuum exhaust tube; 9. a seal ring; 10. an inner cavity.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-6, the present invention provides a technical solution: a method for storing and transporting cells at normal temperature comprises the following steps:
step one, carrying out integral internal and external disinfection treatment on cell storage and transportation equipment;
step two, carrying out integral internal and external disinfection treatment on the glass tube 6, then introducing cell storage and transportation liquid into the glass tube 6, putting the stored and transported cells into the cell storage and transportation liquid in the glass tube 6, blocking a port of the glass tube 6 by using a blocking block 19, and putting the glass tube 6 filled with the cells and the cell storage and transportation liquid into a placing rack 11 in a fixing rack 31;
step three, the surface of the glass tube 6 is in contact with a sponge mat 13 on the inner wall of the U-shaped tube 12, meanwhile, the bottom end of the glass tube 6 is in contact with an arc-shaped groove on the top of a rubber seat 15, a thread block 17 is used for being in threaded connection with the inside of the placing frame 11, a heat insulation block 18 at the bottom of the thread block 17 presses down a blocking block 19 at the port of the glass tube 6, the bottom end of the glass tube 6 exerts downward force on the rubber seat 15, a sliding block 14 is arranged at the bottom of the rubber seat 15, the sliding block 14 generates downward pressure, a spring 16 is stressed to contract, the whole glass tube 6 moves downwards in the U-shaped tube 12 until the thread block 17 is completely in threaded connection with the inside of the placing frame 11, and the whole glass tube 6 enters;
step four, sealing and installing the protection plate 32 and the fixing frame 31, then sealing the sealing part 33 inside the protection plate 32 and the protection plate 32, and vacuumizing the space between the protection plate 32 and the fixing frame 31 through the vacuum exhaust pipe 8;
fifthly, the fixing frame 31 is placed in the storage and transportation box 4, the concave parts on the two sides of the fixing frame 31 and the convex parts on the front and back surfaces are respectively in sliding connection with the limiting blocks 24 on the periphery of the inner wall of the first heat-insulating sleeve 21, meanwhile, the middle part of the surface of the fixing frame 31 is in interference fit connection with the inside of the rubber positioning frame 25 in the first heat-insulating sleeve 21, the fixing frame 31 is limited and installed in the storage and transportation box 4, finally the movable cover 5 is covered, the bottom of the movable cover 5 and the top of the storage and transportation box 4 are sealed through the sealing ring 9, finally, the inside of the storage and transportation box 4 is vacuumized through the vacuum exhaust tube 8 in the top of the movable cover 5, the storage and transportation box 4 is.
The cell storage and transportation liquid is a compound liquid of normal saline, ringer's liquid and compound electrolyte injection.
The cell storage and transportation equipment comprises a storage and transportation box 4, a movable cover 5 and glass tubes 6, wherein one side of the top of the storage and transportation box 4 is hinged with the movable cover 5 through hinges, sponge sleeves 7 are arranged on the two sides of the movable cover 5 and the storage and transportation box 4, a placement mechanism 1 is arranged in the storage and transportation box 4, a plurality of glass tubes 6 are arranged in the placement mechanism 1, a heat insulation mechanism 2 and a protection mechanism 3 are respectively arranged in the storage and transportation box 4, and the protection mechanism 3 is positioned in the heat insulation mechanism 2; the heat insulation mechanism 2 comprises a first heat insulation sleeve 21, a second heat insulation sleeve 22 and a rubber pad 23, the first heat insulation sleeve 21 is arranged inside the storage and transportation box 4, the surface of the first heat insulation sleeve 21 is fixedly connected with the inner wall of the storage and transportation box 4, the rubber pad 23 is arranged on the periphery of the inner wall of the first heat insulation sleeve 21, the periphery of the inner wall of the rubber pad 23 is provided with a limiting block 24, the middle part inside the rubber pad 23 is provided with a rubber positioning frame 25, the inside of the movable cover 5 is provided with the second heat insulation sleeve 22, and the surface of the second heat insulation sleeve 22 is fixedly connected with the inner wall of the movable cover; the protection mechanism 3 comprises a fixed frame 31, protection plates 32 and sealing parts 33, concave parts are arranged on two sides of the fixed frame 31, convex parts are arranged on two sides of the front surface and the back surface of the fixed frame 31, the concave parts and the convex parts are respectively connected with limiting blocks 24 on the periphery of the inner wall of the rubber pad 23 in a sliding mode, the surface of the fixed frame 31 is in interference fit with the inside of the rubber positioning frame 25, the protection plates 32 are arranged on the top of the fixed frame 31, the periphery of the inside of the protection plates 32 is hinged with the sealing parts 33 through hinges, sealing spaces are formed among the protection plates 32, the sealing parts 33 and the inside of the fixed frame 31, and four placing mechanisms 1 are symmetrically arranged inside the fixed frame; the placing mechanism 1 comprises a placing frame 11, a U-shaped pipe 12, a sponge cushion 13, a sliding block 14, a rubber seat 15, a spring 16, a thread block 17 and a heat insulation block 18, the placing frame 11 is arranged inside a fixing frame 31, the U-shaped pipe 12 is arranged on two sides inside the placing frame 11, the sponge cushion 13 is arranged on the inner wall of the U-shaped pipe 12, the sliding block 14 is connected inside the U-shaped pipe 12 in a sliding mode, the rubber seat 15 is arranged on the top of the sliding block 14, an arc-shaped groove is formed in the top of the rubber seat 15, the glass pipe 6 is arranged inside the U-shaped pipe 12, the bottom end of the glass pipe 6 is in contact with the inner wall of the arc-shaped groove, the thread block 17 is connected to the inner thread of the fixing frame 31; a block 19 is arranged at the top end of the U-shaped pipe 12, the top of the block 19 is in contact with the bottom of the heat insulation block 18, a spring 16 is arranged at the bottom of the inner wall of the U-shaped pipe 12, and the top end of the spring 16 is fixedly connected with the bottom of the sliding block 14; the vacuum extraction pipes 8 are arranged inside the movable cover 5 and the protection plate 32, the sealing ring 9 is arranged between the bottom of the movable cover 5 and the top of the storage and transportation box 4, the inner cavities 10 are formed inside the first heat insulation sleeve 21, the second heat insulation sleeve 22, the U-shaped pipe 12 and the heat insulation block 18, and the refrigerant is filled inside the inner cavities 10.
The use method of the cell storage and transportation equipment comprises the following steps:
carrying out integral internal and external sterilization treatment on the glass tube 6, then introducing cell storage and transportation liquid into the glass tube 6, putting the stored and transported cells into the cell storage and transportation liquid in the glass tube 6, blocking the port of the glass tube 6 by using the blocking block 19, and putting the glass tube 6 filled with the cells and the cell storage and transportation liquid into the placing frame 11 in the fixing frame 31;
the surface of the glass tube 6 is in contact with a sponge cushion 13 on the inner wall of the U-shaped tube 12, meanwhile, the bottom end of the glass tube 6 is in contact with an arc-shaped groove on the top of a rubber seat 15, a thread block 17 is used for being in threaded connection with the inside of the placing frame 11, a heat insulation block 18 at the bottom of the thread block 17 presses down a blocking block 19 at the end opening of the glass tube 6, the bottom end of the glass tube 6 applies downward force to the rubber seat 15, a sliding block 14 is arranged at the bottom of the rubber seat 15, the sliding block 14 generates downward pressure, a spring 16 is stressed to contract, the whole glass tube 6 moves downward in the U-shaped tube 12 until the thread block 17 is completely in threaded connection with the inside of the placing frame 11, and the whole glass tube 6;
sealing and installing the protection plate 32 and the fixing frame 31, then sealing the sealing part 33 inside the protection plate 32 and the protection plate 32, and vacuumizing the space between the protection plate 32 and the fixing frame 31 through the vacuum exhaust pipe 8;
the fixed frame 31 is placed in the storage and transportation box 4, the concave parts on the two sides of the fixed frame 31 and the convex parts on the front and back surfaces are respectively in sliding connection with the limiting blocks 24 on the periphery of the inner wall of the first heat-insulating sleeve 21, meanwhile, the middle part of the surface of the fixed frame 31 is in interference fit connection with the inside of the rubber positioning frame 25 in the first heat-insulating sleeve 21, the fixed frame 31 is limited and installed in the storage and transportation box 4, finally, the movable cover 5 is covered, the bottom of the movable cover 5 and the top of the storage and transportation box 4 are sealed through the sealing ring 9, finally, the inside of the storage and transportation box 4 is vacuumized through the vacuum exhaust tube 8 on the top of the movable cover 5, the storage and transportation box 4 is.
The working principle is that when the cell storage and transportation device is used, the glass tube 6 is disinfected integrally and internally, then cell storage and transportation liquid is introduced into the glass tube 6, the stored and transported cells are put into the cell storage and transportation liquid in the glass tube 6, the end opening of the glass tube 6 is blocked by the blocking block 19, and the glass tube 6 filled with the cells and the cell storage and transportation liquid is placed in the placing rack 11 in the fixing rack 31;
the surface of the glass tube 6 is in contact with a sponge cushion 13 on the inner wall of the U-shaped tube 12, meanwhile, the bottom end of the glass tube 6 is in contact with an arc-shaped groove on the top of a rubber seat 15, a thread block 17 is used for being in threaded connection with the inside of the placing frame 11, a heat insulation block 18 at the bottom of the thread block 17 presses down a blocking block 19 at the end opening of the glass tube 6, the bottom end of the glass tube 6 applies downward force to the rubber seat 15, a sliding block 14 is arranged at the bottom of the rubber seat 15, the sliding block 14 generates downward pressure, a spring 16 is stressed to contract, the whole glass tube 6 moves downward in the U-shaped tube 12 until the thread block 17 is completely in threaded connection with the inside of the placing frame 11, and the whole glass tube 6;
sealing and installing the protection plate 32 and the fixing frame 31, then sealing the sealing part 33 inside the protection plate 32 and the protection plate 32, and vacuumizing the space between the protection plate 32 and the fixing frame 31 through the vacuum exhaust pipe 8;
the fixed frame 31 is placed in the storage and transportation box 4, the concave parts on the two sides of the fixed frame 31 and the convex parts on the front and back surfaces are respectively in sliding connection with the limiting blocks 24 on the periphery of the inner wall of the first heat-insulating sleeve 21, meanwhile, the middle part of the surface of the fixed frame 31 is in interference fit connection with the inside of the rubber positioning frame 25 in the first heat-insulating sleeve 21, the fixed frame 31 is limited and installed in the storage and transportation box 4, finally, the movable cover 5 is covered, the bottom of the movable cover 5 and the top of the storage and transportation box 4 are sealed through the sealing ring 9, finally, the inside of the storage and transportation box 4 is vacuumized through the vacuum exhaust tube 8 on the top of the movable cover 5, the storage and transportation box 4 is.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. A method for storing and transporting cells at normal temperature is characterized in that: the method comprises the following steps:
step one, carrying out integral internal and external disinfection treatment on cell storage and transportation equipment;
secondly, sterilizing the inside and outside of the glass tube (6) integrally, then introducing cell storage and transportation liquid into the glass tube (6), putting the stored and transported cells into the cell storage and transportation liquid in the glass tube (6), blocking a port of the glass tube (6) by using a blocking block (19), and putting the glass tube (6) filled with the cells and the cell storage and transportation liquid into a placing rack (11) in a fixing rack (31);
step three, the surface of the glass tube (6) is in contact with a sponge mat (13) on the inner wall of the U-shaped tube (12), meanwhile, the bottom end of the glass tube (6) is in contact with an arc-shaped groove on the top of a rubber seat (15), a thread block (17) is in threaded connection with the inside of a placing frame (11), a heat insulation block (18) at the bottom of the thread block (17) presses down a block (19) at the port of the glass tube (6), the bottom end of the glass tube (6) exerts downward force on the rubber seat (15), a sliding block (14) is arranged at the bottom of the rubber seat (15), the sliding block (14) generates downward pressure, a spring (16) is stressed to contract, the whole glass tube (6) moves downwards in the U-shaped tube (12) until the thread block (17) is completely in threaded connection with the inside of the placing frame (11), and the whole glass tube (6) enters the inside of the U-shaped tube (12);
fourthly, sealing and installing the protection plate (32) and the fixing frame (31), then sealing a sealing part (33) inside the protection plate (32) and the protection plate (32), and vacuumizing the space between the protection plate (32) and the fixing frame (31) through a vacuum exhaust pipe (8);
and fifthly, placing the fixing frame (31) into the storage and transportation box (4), enabling the concave parts on two sides of the fixing frame (31) and the convex parts on the front and back surfaces to be respectively connected with the limiting blocks (24) on the periphery of the inner wall of the first heat-insulating sleeve (21) in a sliding mode, enabling the middle part of the surface of the fixing frame (31) to be connected with the inside of the rubber positioning frame (25) in the first heat-insulating sleeve (21) in an interference fit mode, completing limiting installation of the fixing frame (31) in the storage and transportation box (4), finally covering the movable cover (5), sealing the bottom of the movable cover (5) and the top of the storage and transportation box (4) through a sealing ring (9), finally conducting vacuumizing treatment on the inside of the storage and transportation box (4) through a vacuum exhaust pipe (8) in the top of the movable cover (5), transporting the storage and transportation box (4), and completing.
2. The method of claim 1, wherein the method comprises: the cell storage and transportation liquid is a compound liquid of normal saline, ringer's liquid and compound electrolyte injection.
3. The method of claim 1, wherein the method comprises: the cell storage and transportation equipment comprises a storage and transportation box (4), a movable cover (5) and glass tubes (6), wherein the movable cover (5) is hinged to one side of the top of the storage and transportation box (4) through hinges, sponge sleeves (7) are arranged on the movable cover (5) and the two sides of the storage and transportation box (4), a placement mechanism (1) is arranged inside the storage and transportation box (4), a plurality of glass tubes (6) are arranged inside the placement mechanism (1), a heat insulation mechanism (2) and a protection mechanism (3) are respectively arranged inside the storage and transportation box (4), and the protection mechanism (3) is located inside the heat insulation mechanism (2).
4. The method of claim 3, wherein the method comprises: the heat insulation mechanism (2) comprises a first heat insulation sleeve (21), a second heat insulation sleeve (22) and a rubber pad (23), the first heat insulation sleeve (21) is arranged inside the storage and transportation box (4), the surface of the first heat insulation sleeve (21) is fixedly connected with the inner wall of the storage and transportation box (4), the rubber pad (23) is arranged on the periphery of the inner wall of the first heat insulation sleeve (21), limiting blocks (24) are arranged on the periphery of the inner wall of the rubber pad (23), a rubber positioning frame (25) is arranged in the middle of the inside of the rubber pad (23), the second heat insulation sleeve (22) is arranged inside the movable cover (5), and the surface of the second heat insulation sleeve (22) is fixedly connected with the inner wall of the movable cover (5).
5. The method of claim 3, wherein the method comprises: protection machanism (3) are including mount (31), guard plate (32), sealing (33), the concave part has all been seted up to the both sides of mount (31), and mount (31) openly all is provided with the convex part with the both sides at the back, concave part and convex part respectively with rubber pad (23) inner wall stopper (24) sliding connection all around, and be interference fit between the surface of mount (31) and the inside of rubber locating rack (25), the top of mount (31) is provided with guard plate (32), and all hinges through the hinge around guard plate (32) inside has sealing (33), form confined space between the inside of guard plate (32), sealing (33) and mount (31), the inside symmetry that placing mechanism (1) is located mount (31) is provided with four.
6. The method of claim 4, wherein the method comprises: the placing mechanism (1) comprises a placing rack (11), a U-shaped pipe (12), a spongy cushion (13), a sliding block (14), a rubber seat (15), a spring (16), a thread block (17) and a heat insulation block (18), the placing rack (11) is arranged in the fixing frame (31), and both sides of the interior of the placing rack (11) are provided with U-shaped tubes (12), the inner walls of the U-shaped tubes (12) are provided with sponge cushions (13), a sliding block (14) is connected inside the U-shaped pipe (12) in a sliding way, a rubber seat (15) is arranged at the top of the sliding block (14), the top of the rubber seat (15) is provided with an arc-shaped groove, the glass tube (6) is arranged inside the U-shaped tube (12), and the bottom of the glass tube (6) is contacted with the inner wall of the arc-shaped groove, the internal thread of the fixing frame (31) is connected with a thread block (17), and the bottom of the thread block (17) is provided with a heat insulation block (18).
7. The method of claim 6, wherein the method comprises: the heat insulation block is characterized in that a blocking block (19) is arranged at the top end of the U-shaped pipe (12), the top of the blocking block (19) is in contact with the bottom of the heat insulation block (18), a spring (16) is arranged at the bottom of the inner wall of the U-shaped pipe (12), and the top end of the spring (16) is fixedly connected with the bottom of the sliding block (14).
8. The method of claim 6, wherein the method comprises: the vacuum extraction pipe (8) is arranged inside the movable cover (5) and the protection plate (32), the sealing ring (9) is arranged between the bottom of the movable cover (5) and the top of the storage and transportation box (4), the inner cavity (10) is formed inside the first heat insulation sleeve (21), the second heat insulation sleeve (22), the U-shaped pipe (12) and the heat insulation block (18), and a refrigerant is filled inside the inner cavity (10).
9. The method of claim 1, wherein the method comprises: the use method of the cell storage and transportation equipment comprises the following steps:
the inside and outside disinfection treatment of the glass tube (6) is carried out integrally, then cell storage and transportation liquid is introduced into the glass tube (6), the stored and transported cells are put into the cell storage and transportation liquid in the glass tube (6), a blocking block (19) is used for blocking the port of the glass tube (6), and the glass tube (6) filled with the cells and the cell storage and transportation liquid is put into a placing rack (11) in a fixing rack (31);
the surface of a glass tube (6) is in contact with a sponge mat (13) on the inner wall of a U-shaped tube (12), meanwhile, the bottom end of the glass tube (6) is in contact with an arc-shaped groove on the top of a rubber seat (15), a thread block (17) is in threaded connection with the inside of a placing frame (11), a heat insulation block (18) at the bottom of the thread block (17) presses down a blocking block (19) at the port of the glass tube (6), the bottom end of the glass tube (6) exerts downward force on the rubber seat (15), a sliding block (14) is arranged at the bottom of the rubber seat (15), the sliding block (14) generates downward pressure, a spring (16) is stressed to contract, the whole glass tube (6) moves downwards in the U-shaped tube (12) until the thread block (17) is completely in threaded connection with the inside of the placing frame (11), and the whole glass tube (6) enters the inside of the U-shaped tube (12);
sealing and mounting the protection plate (32) and the fixing frame (31), then sealing a sealing part (33) inside the protection plate (32) and the protection plate (32), and vacuumizing the space between the protection plate (32) and the fixing frame (31) through a vacuum exhaust pipe (8);
the fixing frame (31) is placed in the storage and transportation box (4), the concave parts on the two sides and the convex parts on the front and back surfaces of the fixing frame (31) are respectively in sliding connection with the limiting blocks (24) on the periphery of the inner wall of the first heat-insulating sleeve (21), meanwhile, the middle part of the surface of the fixing frame (31) is in interference fit connection with the inside of the rubber positioning frame (25) in the first heat-insulating sleeve (21), the limiting installation of the fixing frame (31) in the storage and transportation box (4) is completed, finally, the movable cover (5) is covered, the bottom of the movable cover (5) and the top of the storage and transportation box (4) are sealed through the sealing ring (9), finally, the inside of the storage and transportation box (4) is vacuumized through the vacuum exhaust tube (8) in the top of the movable cover (5), the storage and transportation box (4) is transported, and.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110516525.0A CN113120390A (en) | 2021-05-12 | 2021-05-12 | Method for storing and transporting cells at normal temperature |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110516525.0A CN113120390A (en) | 2021-05-12 | 2021-05-12 | Method for storing and transporting cells at normal temperature |
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| CN113120390A true CN113120390A (en) | 2021-07-16 |
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| WO1996036437A1 (en) * | 1995-05-15 | 1996-11-21 | Smithkline Beecham Corporation | Vial holder |
| CN209366777U (en) * | 2018-10-30 | 2019-09-10 | 凡知医疗科技(江苏)有限公司 | A kind of separation human peripheral blood mononuclear cell's kit |
| CN209684381U (en) * | 2019-01-16 | 2019-11-26 | 卡替（上海）生物技术股份有限公司 | A kind of cell deepfreeze case |
| CN111887242A (en) * | 2020-08-21 | 2020-11-06 | 卡替(上海)细胞生物技术有限公司 | Normal-temperature cell storage and transportation method and application thereof |
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2021
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996036437A1 (en) * | 1995-05-15 | 1996-11-21 | Smithkline Beecham Corporation | Vial holder |
| CN209366777U (en) * | 2018-10-30 | 2019-09-10 | 凡知医疗科技(江苏)有限公司 | A kind of separation human peripheral blood mononuclear cell's kit |
| CN209684381U (en) * | 2019-01-16 | 2019-11-26 | 卡替（上海）生物技术股份有限公司 | A kind of cell deepfreeze case |
| CN111887242A (en) * | 2020-08-21 | 2020-11-06 | 卡替(上海)细胞生物技术有限公司 | Normal-temperature cell storage and transportation method and application thereof |
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