CN220402862U - Novel cell liquid nitrogen cryopreservation box - Google Patents

Abstract

The utility model relates to the technical field of cell cryopreservation, in particular to a novel cell liquid nitrogen cryopreservation box. Including the case, the inside sliding connection on case top has the apron, and the inside of case is fixed with the baffle, and the inside of case forms a plurality of storage chamber through the baffle, and the inside equipartition in storage chamber is installed and is used for the positioning mechanism of centre gripping, and every positioning mechanism includes locating plate, rubber pad and reset spring, and the one end of locating plate is fixed with reset spring, and reset spring is fixed with storage chamber, and the other end of locating plate is fixed with the rubber pad. According to the novel cell liquid nitrogen cryopreservation box, the partition plates are fixed in the storage box, the storage cavities for storing cell cryopreservation pipes are formed through the partition plates, meanwhile, the cover plate capable of sliding in a translation mode is arranged at the top of the storage box, the top of the cell cryopreservation pipes is limited through the cover plate, and the cell cryopreservation pipes with different diameters are fixed and stable in the storage cavities through the positioning mechanism.

Description

Novel cell liquid nitrogen cryopreservation box

Technical Field

The utility model relates to the technical field of cell cryopreservation, in particular to a novel cell liquid nitrogen cryopreservation box.

Background

With the development of life science and technology, cells are used as the most direct and effective experimental carrier in various aspects of scientific research: such as medicine screening, cell therapy, drug resistance research, toxicology research and the like, so that the culture and preservation of cells have important significance. Cell cryopreservation is one of the main methods for cell preservation. The freezing technology is used to store the cells in liquid nitrogen at-196 deg.c to separate the cells from growth state temporarily and store their cell characteristics, so that the cells are recovered for experiment when needed. And a certain amount of cells are moderately preserved, so that the cells can be prevented from being lost due to pollution or other unexpected events of the cells being cultured, and the effects of preserving the cells and preserving the cells for a long time are achieved.

As disclosed in patent (CN 202221690217.6), a cell liquid nitrogen freezing box solves the problems that 1, taking out a freezing frame from liquid nitrogen, taking out the freezing box, putting on the hand with anti-freezing gloves, and the freezing box is not easy to open; 2. when the forceps are used for taking and placing cells, as the cell freezing pipes are closely arranged in the freezing box, a pipe of cells is upwards pulled out from the inside to resuscitate the cells with high difficulty; 3. the freezing box is provided with a liquid inlet and outlet hole, and liquid nitrogen can not enter and flow out of the inside of the freezing box rapidly; 4. the lid of present design is all directly covered, and box inside hole site interval is tighter, because freeze the pipe and do not chucking, can be because buoyancy is too big in liquid nitrogen, open the lid, freeze the technical problem that the pipe can possibly scatter.

When the above technology is used, the following technical problems are found in the prior art: the freezing grooves of the existing freezing box are of equal specification, only a cell freezing pipe of a single specification can be frozen, the cell freezing pipes of different sizes cannot be frozen, the applicability is low, no fixed structure exists in the freezing grooves of the existing freezing box, when the freezing box is stored and taken out of the liquid nitrogen tank, inclined spilling of the cell freezing pipe often occurs due to shaking, and therefore, a novel cell liquid nitrogen freezing box is designed and used for providing another technical scheme for the technical problems.

Disclosure of Invention

Based on this, it is necessary to provide a novel cell liquid nitrogen freezes and deposits the box to aim at above-mentioned technical problem, to deposit the inside fixed baffle of box to form a plurality of depositing the chamber that is used for depositing cell cryopreservation pipe through the baffle, set up at the top of depositing the box simultaneously and can translate gliding apron, it is spacing to carry out the top of cell cryopreservation pipe through the apron, and it is fixed firm to deposit the inside of chamber to the cell cryopreservation pipe of different diameters through positioning mechanism.

In order to solve the technical problems, the utility model adopts the following technical scheme:

the utility model provides a novel cell liquid nitrogen freezes deposits box, includes the case, the inside sliding connection on case top has the apron, the inside of case is fixed with the baffle, the inside of case forms a plurality of storage chamber through the baffle, the inside equipartition in storage chamber is installed and is used for the positioning mechanism of centre gripping, every positioning mechanism includes locating plate, rubber pad and reset spring, the one end of locating plate is fixed with reset spring, reset spring is fixed with the storage chamber, the other end of locating plate is fixed with the rubber pad.

As a preferred implementation mode of the novel cell liquid nitrogen freezing storage box, the storage box and the partition plate are made of polycarbonate plastics.

As a preferred embodiment of the novel cell liquid nitrogen freezing box provided by the utility model, a buckling groove is formed in one end of the top of the cover plate.

As a preferred embodiment of the novel cell liquid nitrogen freezing storage box provided by the utility model, first through holes are uniformly distributed on the outer side of the storage box and at the bottom of the cover plate.

As a preferred embodiment of the novel cell liquid nitrogen freezing storage box provided by the utility model, the inside of the bottom end of the storage box and the position corresponding to the storage cavity are provided with the second through holes.

As a preferred implementation mode of the novel cell liquid nitrogen freezing box provided by the utility model, one end of the storage box is provided with a fixing mechanism for positioning the cover plate, the fixing mechanism comprises a fixing shell, a squeezing plate and a rotating shaft, the fixing shell is fixed with the storage box, the squeezing plate is connected inside the fixing shell in a sliding manner, the rotating shaft is fixed inside one end of the squeezing plate and inside the fixing shell, the rotating shaft is connected with the fixing shell in a rotating manner, and an extrusion inclined plane is formed in the top of the squeezing plate, close to one end of the storage box.

As a preferred implementation mode of the novel cell liquid nitrogen freezing box provided by the utility model, a limiting component used for positioning the position of the extrusion plate is arranged in the fixing shell, the limiting component comprises a pull rod, a reset pressure spring and a limiting plate, the limiting plate is connected in a sliding manner in the fixing shell, an inclined plane is formed at one end of the limiting plate, which is far away from one side of the extrusion plate, one end of the limiting plate is fixedly provided with a pull rod, the pull rod is connected with the fixing shell in a sliding manner, and the reset pressure spring is sleeved between the limiting plate and the fixing shell on the outer side of the pull rod.

It can be clearly seen that the technical problems to be solved by the present application must be solved by the above-mentioned technical solutions of the present application.

Meanwhile, through the technical scheme, the utility model has at least the following beneficial effects:

according to the novel cell liquid nitrogen cryopreservation box, the partition plates are fixed in the storage box, a plurality of storage cavities for storing cell cryopreservation pipes are formed through the partition plates, meanwhile, the top of the storage box is provided with the cover plate capable of horizontally moving and sliding, the top of the cell cryopreservation pipes is limited through the cover plate, and the inside of the storage cavities for storing the cell cryopreservation pipes with different diameters is fixed and stable through the positioning mechanism;

through the cooperation of the positioning plate, the rubber pad and the reset spring, when the cell freezing and storing tube is stored in the storage cavity, the positioning plate is pressed by the cell freezing and storing tube and the reset spring is compressed and reset, so that the positioning plate can clamp the cell freezing and storing tubes with different sizes through the rubber pad;

through the cooperation on fixed shell, stripper plate and extrusion inclined plane for the stripper plate can be spacing to the apron in the inside position of case through the extrusion inclined plane at the inside rotation of fixed shell, makes the apron can be to the more firm of case closure, can carry out better spacing to the inside cell cryopreservation pipe of case, and guarantees the better cryopreservation effect of cell.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings required for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present utility model, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic overall structure of a first embodiment of the present utility model;

FIG. 2 is a schematic view of the internal structure of the case of the present utility model;

FIG. 3 is a schematic view of the structure of the separator of the present utility model;

FIG. 4 is a schematic overall structure of a second embodiment of the present utility model;

fig. 5 is a schematic view showing the internal structure of the fixing case of the present utility model.

In the figure: 1. a storage box; 2. a cover plate; 3. a first through hole; 4. a buckling groove; 5. a partition plate; 6. a second through hole; 7. a positioning plate; 8. a rubber pad; 9. a return spring; 10. a fixed case; 11. an extrusion plate; 12. extruding the inclined plane; 13. a rotating shaft; 14. a pull rod; 15. resetting a pressure spring; 16. a limiting plate; 17. an inclined surface.

Detailed Description

The present utility model will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present utility model more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the utility model.

As described in the background art, the freezing grooves of the existing freezing box are of equal specification, and can only freeze a cell freezing tube of a single specification, but cannot freeze cell freezing tubes of different sizes, so that the applicability is low, no fixed structure is arranged in the freezing grooves of the existing freezing box, and when the freezing box is stored and taken out from the liquid nitrogen tank, the cell freezing tube is often inclined and spilled due to shaking, so that the cell freezing tube is difficult to find and take.

In order to solve the technical problem, the utility model provides a novel cell liquid nitrogen cryopreservation box.

Specifically, referring to fig. 1 to 3, the freezing box specifically includes:

the storage box 1 is characterized in that the cover plate 2 is connected inside the top end of the storage box 1 in a sliding manner, the partition plate 5 is fixed inside the storage box 1, and a plurality of storage cavities are formed inside the storage box 1 through the partition plate 5;

the positioning mechanism is positioned in the storage cavity.

According to the novel cell liquid nitrogen cryopreservation box provided by the utility model, the partition board 5 is fixed in the storage box 1, a plurality of storage cavities for storing cell cryopreservation pipes are formed through the partition board 5, meanwhile, the cover plate 2 capable of horizontally moving and sliding is arranged at the top of the storage box 1, the top of the cell cryopreservation pipes is limited through the cover plate 2, and the cell cryopreservation pipes with different diameters are fixed and stable in the storage cavities through the positioning mechanism.

In order to make the person skilled in the art better understand the solution of the present utility model, the technical solution of the embodiment of the present utility model will be clearly and completely described below with reference to the accompanying drawings.

It should be noted that, under the condition of no conflict, the embodiments of the present utility model and the features and technical solutions in the embodiments may be combined with each other.

It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.

Example 1

Referring to fig. 1-3, a novel cell liquid nitrogen freezing box comprises a storage box 1, wherein the inside of the top end of the storage box 1 is slidably connected with a cover plate 2, a buckling groove 4 is formed in the inside of one end of the top of the cover plate 2, so that the storage box 1 can be conveniently driven to slide in the cover plate 2 through the buckling groove 4, first through holes 3 are uniformly formed in the outer side of the storage box 1 and in the bottom of the cover plate 2, so that liquid nitrogen in the liquid nitrogen tank can be conveniently and rapidly flowed into the freezing box through the first through holes 3 when the freezing box is placed in the liquid nitrogen tank, and the inside of the freezing box is rapidly filled with liquid nitrogen; when the freezing box is taken out from the liquid nitrogen tank, liquid nitrogen in the freezing box can flow out and be emptied rapidly through the first through hole 3;

the baffle plate 5 is fixed in the storage box 1, a plurality of storage cavities are formed in the storage box 1 through the baffle plate 5, so that the cell freezing storage tube can be placed in the storage cavities, and the second through holes 6 are formed in the storage box 1 at the bottom end and at the positions corresponding to the storage cavities, so that when the freezing storage box is placed in the liquid nitrogen tank through the second through holes 6, liquid nitrogen in the liquid nitrogen tank can quickly flow into the freezing storage box through the second through holes 6, and the inside of the freezing storage box is quickly filled with the liquid nitrogen; when the freezing box is taken out from the liquid nitrogen tank, liquid nitrogen in the freezing box can flow out and be emptied rapidly through the second through hole 6;

in this embodiment, the storage box 1 and the partition plate 5 are made of polycarbonate plastic, and have good low temperature resistance, impact resistance, water absorption, insulation and aging resistance.

In this embodiment, be equipped with different numbers with depositing the position that the chamber corresponds on the apron 2, can put into fixed depositing the intracavity with the cell cryopreservation pipe in order, can not cause the confusion, improved the efficiency of experiment greatly.

The inside equipartition of depositing the chamber is installed and is used for the positioning mechanism of centre gripping, positioning mechanism can be a plurality of in other embodiments, every positioning mechanism includes locating plate 7, rubber pad 8 and reset spring 9, the one end of locating plate 7 is fixed with reset spring 9, reset spring 9 is fixed with depositing the chamber, make the removal of locating plate 7 can extrude reset spring 9, and carry out certain support to locating plate 7 through reset spring 9's rebound, the other end of locating plate 7 is fixed with rubber pad 8, can not lead to cell cryopreservation pipe damage when making locating plate 7 to cell cryopreservation pipe centre gripping, thereby fix the cell cryopreservation pipe of equidimension and avoid the slope to spill;

the frozen box that this embodiment provided, deposit the inside fixed baffle 5 of box 1, and form a plurality of depositing the chamber that is used for depositing cell frozen tube through baffle 5, set up at the top of box 1 simultaneously and carry out translation gliding apron 2, it is spacing to the top of cell frozen tube through apron 2, when cell frozen tube deposited the inside of depositing the chamber, arc through cell frozen tube bottom extrudees rubber pad 8, make rubber pad 8 drive reset spring 9 remove and let reset spring 9 compress, then carry out certain reset to locating plate 7 through reset spring 9's rebound, make locating plate 7 drive rubber pad 8 and cell frozen tube outer wall tightly laminate, thereby cell frozen tube that can fix different sizes and avoid the slope to spill and leak, the searching and taking of cell frozen tube are convenient for.

Example two

Referring to fig. 4-5, a novel cell liquid nitrogen freezing box is disclosed, one end of a storage box 1 is provided with a fixing mechanism for positioning a cover plate 2, the fixing mechanism comprises a fixing shell 10, a squeezing plate 11 and a rotating shaft 13, the fixing shell 10 is fixed with the storage box 1, the fixing shell 10 is positioned at one end of the cover plate 2, which can be separated from the inside of the storage box 1 in a sliding manner, the squeezing plate 11 is slidingly connected inside the fixing shell 10, one end of the squeezing plate 11 is fixedly provided with the rotating shaft 13, the rotating shaft 13 is rotationally connected with the fixing shell 10, the squeezing plate 11 can rotate inside the fixing shell 10, one end, close to the storage box 1, of the top of the squeezing plate 11 is provided with a squeezing inclined surface 12, and the cover plate 2 can be squeezed through the squeezing inclined surface 12, so that the cover plate 2 cannot be separated from the inside the storage box 1;

the limiting assembly used for positioning the position of the extrusion plate 11 is arranged in the fixed shell 10, and comprises a pull rod 14, a reset pressure spring 15 and a limiting plate 16, wherein the limiting plate 16 is slidably connected in the fixed shell 10, so that the sliding of the limiting plate 16 in the fixed shell 10 cannot be separated, an inclined surface 17 is formed at one end of the limiting plate 16, which is far away from the extrusion plate 11, and the inclined surface 17 is positioned at one end of the limiting plate 16, which is close to the storage box 1, so that the extrusion plate 11 rotates outside the rotating shaft 13 and extrudes the limiting plate 16 through the inclined surface 17, and after the sliding of the limiting plate 16 in the fixed shell 10 enables the extrusion plate 11 to be in contact with the cover plate 2 to position the cover plate 2, the position of the extrusion plate 11 is limited through the limiting plate 16;

one end of the limiting plate 16 is fixed with a pull rod 14, the pull rod 14 is in sliding connection with the fixed shell 10, meanwhile, the sliding of the pull rod 14 does not influence the rotation of the extrusion plate 11 in the fixed shell 10, and a reset pressure spring 15 is sleeved outside the pull rod 14 and between the limiting plate 16 and the fixed shell 10, so that the reset pressure spring 15 can be compressed by the movement of the limiting plate 16 and reset through the rebound of the reset pressure spring 15;

the cryopreservation box that this embodiment provided, when using, through manual pulling stripper plate 11, make stripper plate 11 rotate through the rotation connection of axis of rotation 13 with fixed shell 10, extrude apron 2 through extrusion inclined plane 12 through the rotation of stripper plate 11, make apron 2 more closed in the inside of case 1, simultaneously the rotation of stripper plate 11 extrudees limiting plate 16 through inclined plane 17, make limiting plate 16 slide and drive pull rod 14 and remove in the inside of fixed shell 10, compress reset pressure spring 15 simultaneously, after stripper plate 11 rotates and the laminating of fixed shell 10, limiting plate 16 is reset the position of stripper plate 11 through the rebound after reset pressure spring 15 compression spacing, make stripper plate 11 fix the position of apron 2, when apron 2 breaks away from through the slip, let limiting plate 16 break away from limiting to stripper plate 11 through pulling pull rod 14, then rotate stripper plate 11 break away from apron 2.

The use process of the novel cell liquid nitrogen cryopreservation box provided by the utility model is as follows:

the cell that needs to freeze is added in the cell cryopreservation pipe, then put the cell cryopreservation pipe into the storage chamber that is used for depositing the cell cryopreservation pipe by the inside fixed baffle 5 of case 1 and form, set up at the top of case 1 simultaneously and can translate gliding apron 2, it is spacing to the top of cell cryopreservation pipe through apron 2, when the cell cryopreservation pipe is deposited in the inside of depositing the chamber, extrude rubber pad 8 through the arc of cell cryopreservation pipe bottom, make rubber pad 8 drive reset spring 9 remove and let reset spring 9 compress, then carry out certain reset to locating plate 7 through reset spring 9's rebound, make locating plate 7 drive rubber pad 8 and cell cryopreservation pipe outer wall tightly laminate, thereby can fix the cell cryopreservation pipe of equidimension and avoid the slope to spill. Then through manual pulling stripper plate 11 for stripper plate 11 rotates through the rotation connection of axis of rotation 13 and fixed shell 10, extrude apron 2 through extrusion inclined plane 12 through the rotation of stripper plate 11, make apron 2 more closed in the inside of case 1, simultaneously the rotation of stripper plate 11 extrudees limiting plate 16 through inclined plane 17, make limiting plate 16 slide and drive pull rod 14 and remove in the inside of fixed shell 10, simultaneously compress reset pressure spring 15, after stripper plate 11 rotates and the laminating of fixed shell 10, limiting plate 16 is through the rebound reset after reset pressure spring 15 compression to the position of stripper plate 11 spacing, make stripper plate 11 fix the position of apron 2 and with the freezing box closure, thereby the cell freezing pipe of this freezing box inside just is fixed and is placed and finishes. And finally, placing the cryopreservation box containing the cells to be cryopreserved into a liquid nitrogen tank, and completing the liquid nitrogen preservation process of the cells.

When the frozen cells need to be recovered and taken out, the frozen box is taken out of the liquid nitrogen tank, then the limiting plate 16 is separated from the limiting of the extrusion plate 11 by pulling the pull rod 14, and then the extrusion plate 11 is rotated to be separated from the cover plate 2, so that the cover plate 2 can be opened, then the number on the cover plate 2 is corresponded, the frozen cells in the corresponding storage cavity can be taken out, and the cells in the frozen cells are subjected to subsequent experiments.

The preferred embodiments of the utility model disclosed above are intended only to assist in the explanation of the utility model. The preferred embodiments are not exhaustive or to limit the utility model to the precise form disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the utility model and the practical application, to thereby enable others skilled in the art to best understand and utilize the utility model. The utility model is limited only by the claims and the full scope and equivalents thereof.

Claims (7)

1. Novel cell liquid nitrogen freezes case, a serial communication port, including case (1), the inside sliding connection on case (1) top has apron (2), the inside of case (1) is fixed with baffle (5), the inside of case (1) forms a plurality of storage chamber through baffle (5), the inside equipartition in storage chamber installs the positioning mechanism that is used for the centre gripping, every positioning mechanism includes locating plate (7), rubber pad (8) and reset spring (9), the one end of locating plate (7) is fixed with reset spring (9), reset spring (9) are fixed with the storage chamber, the other end of locating plate (7) is fixed with rubber pad (8).

2. The novel cell liquid nitrogen freezing box according to claim 1, wherein the storage box (1) and the partition plate (5) are made of polycarbonate plastics.

3. The novel cell liquid nitrogen cryopreservation box according to claim 1, wherein a buckling groove (4) is formed in one end of the top of the cover plate (2).

4. The novel cell liquid nitrogen freezing box according to claim 1, wherein first through holes (3) are uniformly distributed at the outer side of the storage box (1) and at the bottom of the cover plate (2).

5. The novel cell liquid nitrogen freezing box according to claim 1, wherein the second through holes (6) are formed in the bottom end of the storage box (1) and correspond to the storage cavity.

6. The novel cell liquid nitrogen cryopreservation box according to claim 1, characterized in that, the fixed establishment who is used for fixing a position apron (2) is installed to the one end of case (1), fixed establishment includes fixed shell (10), stripper plate (11) and axis of rotation (13), fixed shell (10) are fixed with case (1), the inside sliding connection of fixed shell (10) has stripper plate (11), the inside of stripper plate (11) one end just is located the inside of fixed shell (10) and is fixed with axis of rotation (13), just axis of rotation (13) are connected with the rotation of fixed shell (10), extrusion inclined plane (12) have been seted up near the one end of case (1) at stripper plate (11) top.

7. The novel cell liquid nitrogen cryopreservation box according to claim 6, characterized in that, internally mounted of fixed shell (10) has the spacing subassembly that is used for the position location to stripper plate (11), spacing subassembly includes pull rod (14), pressure spring (15) and limiting plate (16) reset, the inside sliding connection of fixed shell (10) has limiting plate (16), inclined plane (17) have been seted up to one end that stripper plate (11) one side was kept away from to limiting plate (16), one end of limiting plate (16) is fixed with pull rod (14), just pull rod (14) and fixed shell (10) sliding connection, outside and being located between limiting plate (16) and the fixed shell (10) of pull rod (14) cup joint pressure spring (15) reset.