CN220333010U

CN220333010U - Cell culture medium partial shipment device

Info

Publication number: CN220333010U
Application number: CN202320979564.9U
Authority: CN
Inventors: 张彬
Original assignee: Miaoshun Shanghai Biotechnology Co ltd
Current assignee: Miaoshun Shanghai Biotechnology Co ltd
Priority date: 2023-04-26
Filing date: 2023-04-26
Publication date: 2024-01-12
Anticipated expiration: 2033-04-26

Abstract

The utility model discloses a cell culture medium split charging device, which relates to the technical field of cell culture and comprises a base, wherein a workbench and a rotary table are arranged on the base, a liquid storage tank is arranged on the workbench, a stirring device is arranged in the liquid storage tank, a liquid inlet pipe and a liquid outlet pipe are connected onto the liquid storage tank, a switch valve is arranged on the liquid outlet pipe, a mounting groove is formed in the rotary table, a test tube is arranged in the mounting groove, the test tube is arranged on one side of the liquid outlet pipe, a lifting rod is connected onto one side of the workbench, one end of the lifting rod is connected with a telescopic rod, one end of the telescopic rod is connected with a clamping ring, a funnel is arranged in the clamping ring, and the funnel is arranged between the liquid outlet pipe and the test tube. The cell culture medium split charging device provided by the utility model can prevent the liquid of the culture medium from being adhered to the pipe orifice when the culture medium is split charged and poured, is simple to operate and convenient to use, and improves the working efficiency.

Description

Cell culture medium partial shipment device

Technical Field

The utility model relates to the technical field of cell culture, in particular to a cell culture medium split charging device.

Background

Cell culture refers to a method for simulating in vitro in vivo environment asepsis, proper temperature, pH value and certain nutrition conditions, etc. to survive, grow, reproduce and maintain main structure and function. In cell culture experiments, the preparation of culture media is one of the most basic procedures. At present, in the step of split charging of the culture medium, when the culture medium liquid is poured into a test tube, the culture medium liquid is easy to be stained to a tube orifice, so that a cotton plug is easy to be soaked when the test tube is sealed by the cotton plug in the follow-up process, and bacterial pollution is caused.

Therefore, it is desirable to design a cell culture medium dispensing device that avoids having the medium liquid adhere to the nozzle when the medium is dispensed and poured.

Disclosure of Invention

In view of the defects existing at present, the utility model provides a cell culture medium split charging device which can prevent culture medium liquid from being adhered to a pipe orifice when split charging and pouring culture medium, is simple to operate and convenient to use, and improves the working efficiency.

In order to achieve the above purpose, the embodiment of the present utility model adopts the following technical scheme:

the utility model provides a cell culture base partial shipment device, includes the base, be equipped with workstation and carousel on the base, be equipped with the liquid storage pot on the workstation, be equipped with agitating unit in the liquid storage pot, even there are feed liquor pipe and drain pipe on the liquid storage pot, be equipped with the ooff valve on the drain pipe, be equipped with the mounting groove on the carousel, be equipped with the test tube in the mounting groove, drain pipe one side is located to the test tube, workstation one side is even there is the lifter, lifter one end is even there is the telescopic link, telescopic link one end is even there is the holding ring, be equipped with the funnel in the holding ring, the funnel is located between drain pipe and the test tube.

According to one aspect of the utility model, the clamping ring consists of two oppositely arranged arc-shaped rings, and the filter membrane is arranged in the funnel.

According to one aspect of the utility model, the mounting grooves and the test tubes are a plurality of, and are uniformly distributed at intervals in the circumferential direction of the turntable.

According to one aspect of the utility model, one side of the turntable is connected with the middle rotating shaft, the turntable is rotationally connected to the base through the middle rotating shaft, the turntable is provided with gear teeth, the base is provided with the driving device, the driving device is in matched connection with the gear teeth, and the driving device drives the gear teeth to drive the turntable to intermittently rotate.

According to one aspect of the utility model, the cell culture medium split charging device further comprises a master control system, wherein the master control system is respectively connected with the stirring device, the switch valve, the lifting rod, the telescopic rod and the driving device.

According to one aspect of the utility model, the transfer shaft is hollow and tubular, a sensor is arranged in the mounting groove, the sensor is connected with the master control system, and the test tube is arranged on the sensor.

According to one aspect of the utility model, a liquid level switch is arranged in the mounting groove, the liquid level switch is arranged at 1/4 of the height of the test tube, and the liquid level switch is connected with a master control system.

According to one aspect of the utility model, the liquid storage tank is provided with a separation layer, an additive inlet and a liquid level meter, the separation layer is provided with a temperature-adjusting liquid inlet and a temperature-adjusting liquid outlet, and the liquid level meter is connected with a general control system.

According to one aspect of the utility model, a micro-delivery pump is arranged on the liquid outlet pipe, the micro-delivery pump is connected with the master control system, the micro-delivery pump is arranged on one side of the switch valve, and a filter screen is arranged in the liquid outlet pipe.

According to one aspect of the utility model, the sensor is a weight sensor.

The implementation of the utility model has the advantages that: the method comprises the steps of feeding a cell culture medium into a liquid storage tank through a liquid inlet pipe in advance, uniformly stirring by a stirring device, placing a test tube in a mounting groove on a turntable, lifting and lowering a lifting rod and stretching a telescopic rod according to the distance between the test tube and a liquid outlet pipe, stretching the lower end part of the funnel to a certain distance below the pipe orifice of the test tube, and then opening a switching valve to split the culture medium liquid in the liquid storage tank into the test tube; so, this device can avoid letting the culture medium liquid be stained with the mouth of pipe when the partial shipment pours into the culture medium, easy operation, convenient to use. The driving device is arranged to drive the turntable to intermittently rotate, when one test tube is packaged, the other test tube rotates to the corresponding position to continue packaging, and after one period, a worker only needs to take out and replace the test tube; therefore, the device has higher efficiency and more convenient use. By arranging the weight sensor below the test tube, when the culture medium liquid in the test tube reaches 1/4 of the height of the test tube and reaches the weight value preset by the sensor, the master control system controls the switch valve to be closed, so that the culture medium liquid is not poured into the test tube any more; thus, the device can also solve the problem that the liquid is excessive when the existing cell culture medium is split-packed.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present utility model, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

Fig. 1 is a schematic structural view of the present utility model.

The names corresponding to the serial numbers in the figures are as follows:

1. a base; 2. a work table; 3. a turntable; 31. a mounting groove; 32. a middle rotating shaft; 33. gear teeth; 4. a liquid storage tank; 41. a liquid inlet pipe; 42. a liquid outlet pipe; 43. an additive inlet; 44. a temperature-regulating liquid inlet; 45. a temperature-regulating liquid outlet; 5. a stirring device; 51. an electric cylinder; 52. a mounting plate; 53. a stirring motor; 54. a stirring shaft; 55. stirring paddles; 6. a switch valve; 7. a test tube; 8. a lifting rod; 9. a telescopic rod; 10. a clamping ring; 11. a funnel; 12. a driving device; 121. a speed reducing motor; 122. a drive gear; 13. a sensor; 14. a micro-delivery pump; 15. mounting a support plate; 16. and (5) connecting a block.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Example 1

As shown in FIG. 1, a cell culture medium split charging device comprises a base 1, wherein a workbench 2 and a rotary table 3 are arranged on the base 1, the workbench 2 can be in a table shape made of four columns and one plate, and a liquid storage tank 4 is arranged on the workbench 2 and used for storing or modulating cell culture medium; the stirring device 5 is arranged in the liquid storage tank 4 and can be used for further stirring the cell culture medium to prevent precipitation or for modulating, the stirring device 5 can be composed of an electric cylinder 51, a mounting plate 52, a stirring motor 53, a stirring shaft 54 and stirring blades 55, the bottom of the electric cylinder 51 is fixed on the workbench 2, the extending end of the electric cylinder 51 is fixed with the mounting plate 52, the stirring motor 53 is fixed on the mounting plate 52, the stirring shaft 54 is connected to the output end of the stirring motor 53, and a plurality of stirring blades 55 are fixed on the circumference of the stirring shaft 54; the liquid storage tank 4 is connected with a liquid inlet pipe 41 and a liquid outlet pipe 42, the liquid outlet pipe 42 is provided with a switch valve 6, the turntable 3 is provided with a mounting groove 31, a test tube 7 is placed in the mounting groove 31, the mounting groove 31 is a cylindrical groove and is matched with the structure of the test tube 7, and the test tube 7 is arranged below the liquid outlet pipe 42. The bottom of the workbench 2 is connected with a lifting rod 8, a square installation support plate 15 is fixed at the extending end of the lifting rod 8, a telescopic rod 9 is connected to the installation support plate 15, and the lifting rod 8 and the telescopic rod 9 can be electric push rods; the extension end of the telescopic rod 9 is fixed with a block-shaped connecting block 16, the connecting block 16 is fixed with a clamping ring 10, a funnel 11 is arranged in the clamping ring 10, the funnel 11 is a conical funnel 11, and the funnel 11 is arranged between the liquid outlet pipe 42 and the test tube 7. The liquid storage tank 4 can be provided with an interlayer, an additive inlet 43 and a liquid level meter; the interlayer is provided with a temperature-regulating liquid inlet 44 and a temperature-regulating liquid outlet 45 which are respectively used for the inlet and outlet of temperature-regulating liquid, so that the temperature of the culture medium in the liquid storage tank 4 can be conveniently controlled, the solidification of the culture medium is avoided, the blockage and the waste are generated, and if the temperature of the culture medium is too high, the temperature of the culture medium can be properly reduced; disinfectant, clean water, inorganic salt and the like can enter the liquid storage tank 4 from the additive inlet 43; the level gauge can be used to monitor the level of the culture medium in the liquid storage tank 4, and if the culture medium in the liquid storage tank 4 is less or used up, the liquid level gauge can be convenient for personnel to add. The cell culture medium split charging device further comprises a master control system which is respectively connected with the electric cylinder 51, the stirring motor 53, the lifting rod 8, the telescopic rod 9 and the liquid level meter and used for controlling the electric cylinder 51 to stretch and retract, the stirring motor 53 to rotate, the lifting rod 8 to stretch and retract, the telescopic rod 9 to stretch and the liquid level meter to monitor the liquid level and the like. The switch valve 6 can be opened and closed manually or can be controlled to be opened and closed after being connected with the switch valve through a master control system.

In this embodiment, the clamping ring 10 is composed of two oppositely arranged arc-shaped rings, and is in an open circular shape, and the conical part of the funnel 11 is placed between the two arc-shaped rings, so that the funnel 11 can be conveniently installed and can be easily replaced; a filter membrane can be fixed in the funnel 11, and can be adhered and fixed by adopting an adhesive, and the filter membrane is convenient for further filtering the culture medium liquid.

In this embodiment, the liquid outlet pipe 42 may be provided with a micro-pump 14 for conveying the culture medium in the liquid storage tank 4 outwards, the micro-pump 14 is connected with the master control system, the micro-pump 14 is disposed on one side of the switch valve 6, and the liquid outlet pipe 42 may be provided with a filter screen for filtering the output culture medium liquid.

When the culture medium is used, a cell culture medium is prepared according to 70% -99% of water, vitamins, amino acids, glucose, inorganic salts, antibiotics, cell growth factors, bovine serum, recombinant insulin and the like, the cell culture medium is sent into a liquid storage tank 4 through a liquid inlet pipe 41 and is uniformly stirred by a stirring device 5, a test tube 7 is placed in a mounting groove 31 on a turntable 3, the position of a funnel 11 is adjusted by lifting and lowering the test tube 7 and stretching the lower end part of the funnel 11 to a certain distance below the pipe orifice of the test tube 7 by a lifting rod 8 and stretching the telescopic rod 9 according to the interval (vertical height difference and horizontal center distance) between the test tube 7 and the liquid outlet pipe 42, then a switch valve 6 is opened manually or through a general control system, so that the culture medium liquid in the liquid storage tank 4 is split-filled into the test tube 7, after split-filling is finished, the split-filled test tube 7 is taken out by a worker and is put into a new test tube 7 into the mounting groove 31, and the previous procedure is repeated; so, this device can avoid letting the culture medium liquid be stained with the mouth of pipe when the partial shipment pours into the culture medium, easy operation, convenient to use. When the funnel 11 is used for a long time, the funnel can be replaced manually, and is convenient and practical.

Example two

The difference between the first embodiment and the second embodiment is that the number of the mounting grooves 31 and the test tubes 7 is several, and the mounting grooves and the test tubes are uniformly distributed at intervals in the circumferential direction of the turntable 3, and each mounting groove and each test tube is preferably six groups; a middle rotating shaft 32 is integrally fixed in the center of the bottom of the turntable 3, the turntable 3 is rotationally connected to the base 1 through the middle rotating shaft 32, gear teeth 33 are arranged on the turntable 3, a driving device 12 is arranged on the base 1, the driving device 12 is in matched connection with the gear teeth 33, and the gear teeth 33 are driven by the driving device 12 to drive the turntable 3 to intermittently rotate; the driving device 12 can adopt a gear motor 121 with an output end connected with a driving gear 122, the gear motor 121 is connected with a general control system, and the driving gear 122 is meshed with the gear teeth 33 in a matching way.

When the test tube packaging machine is used, six test tubes 7 are placed in the mounting groove 31 in advance, then the corresponding working procedures in the first embodiment are executed, when one test tube 7 is packaged, the main control system controls the turntable 3 to intermittently rotate, so that the other adjacent test tube 7 rotates to the corresponding position to continue packaging, and after one period, after all the six test tubes 7 are packaged, a worker only needs to take out and replace the test tube 7; so, this device can carry out partial shipment to many test tubes 7, and the staff only need take out and change operation such as test tube 7, and efficiency is higher, it is more convenient to use.

Example III

The difference between the embodiment and the second embodiment is that the sensor 13 is arranged at the bottom in the mounting groove 31 and can be used as a weight sensor, the sensor 13 is connected with a general control system, and the test tube 7 is placed on the sensor 13; the middle rotating shaft 32 is hollow and tubular, so that the electric control circuit can be conveniently inserted and connected.

When the test tube 7 is used, the weight value of the culture medium liquid reaching 1/4 of the height of the test tube 7 is set in advance for the sensor 13, then the corresponding working procedure in the second embodiment is executed, when one test tube 7 is split charging, after the culture medium liquid reaches 1/4 of the height of the test tube 7, the sensor 13 transmits a signal to the master control system, the master control system controls the switch valve 6 to close or the micro-delivery pump 14 to stop working, namely the split charging of the cell culture medium is stopped, then the master control system controls the turntable 3 to rotate, the other adjacent test tube 7 is rotated to the corresponding position to continue split charging, and the previous working procedure is repeatedly executed; so, this device can also solve the problem that the liquid is excessive easily poured in when current cell culture medium partial shipment, has solved the liquid of pouring and surpassed 1/4 of test tube 7 height and later in follow-up sterilization link, and the culture medium boiling probably can spread the problem to the bottleneck.

Example IV

The difference between the embodiment and the third embodiment is that the bottom of the installation groove 31 is not provided with a weight sensor, the inner wall of the installation groove 31 is provided with a liquid level switch, the liquid level switch is arranged at 1/4 of the height of the test tube 7, and the liquid level switch is connected with a general control system. When the liquid level switch detects that the liquid in the test tube 7 reaches 1/4 of the height of the test tube 7, a signal is transmitted to the master control system, and a subsequent corresponding procedure is executed.

In practical application, the cell culture medium split charging device can also comprise a voice reminding device or an alarm reminding device which is connected with the master control system; the liquid storage device can be used for reminding a worker when the liquid in the liquid storage tank 4 is less or used up, the liquid in the test tube 7 reaches 1/4 of the height of the test tube 7, and the sub-packaging of the test tubes 7 is completed.

In practical application, the base 1, the workbench 2, the turntable 3, the liquid storage tank 4, the stirring device 5, the liquid inlet pipe 41, the liquid outlet pipe 42, the switch valve 6, the test tube 7, the driving device 12, the interlayer, the additive inlet 43, the liquid level meter and the micro transfer pump 14 are all in the prior art, and will not be described again.

The implementation of the utility model has the advantages that: the cell culture medium is sent into the liquid storage tank 4 through the liquid inlet pipe 41 in advance, and is uniformly stirred by the stirring device 5, then the test tube 7 is placed in the mounting groove 31 on the turntable 3, the position of the funnel 11 is adjusted by lifting the lifting rod 8 and stretching the telescopic rod 9 according to the interval between the test tube 7 and the liquid outlet pipe 42, the lower end part of the funnel 11 stretches into a certain distance below the pipe orifice of the test tube 7, and then the switch valve 6 is opened, so that the culture medium liquid in the liquid storage tank 4 is split into the test tubes 7; so, this device can avoid letting the culture medium liquid be stained with the mouth of pipe when the partial shipment pours into the culture medium, easy operation, convenient to use. The driving device 12 is arranged to drive the turntable 3 to intermittently rotate, when one test tube 7 is packaged, the other test tube 7 rotates to the corresponding position to continue packaging, and after one period, a worker only needs to take out and replace the test tube 7; therefore, the device has higher efficiency and more convenient use. By arranging the weight sensor below the test tube 7, when the culture medium liquid in the test tube 7 reaches 1/4 of the height of the test tube 7 and reaches the weight value preset by the sensor 13, the master control system controls the switch valve 6 to be closed, so that the culture medium liquid is not poured into the test tube 7 any more; thus, the device can also solve the problem that the liquid is excessive when the existing cell culture medium is split-packed.

The foregoing is merely illustrative of the present utility model, and the present utility model is not limited thereto, and any changes or substitutions easily contemplated by those skilled in the art within the technical scope of the present utility model should be included in the scope of the present utility model. Therefore, the protection scope of the present utility model shall be subject to the protection scope of the claims.

Claims

1. Cell culture base partial shipment device, including base (1), be equipped with workstation (2) and carousel (3) on base (1), be equipped with liquid storage pot (4) on workstation (2), be equipped with agitating unit (5) in liquid storage pot (4), even be equipped with feed liquor pipe (41) and drain pipe (42) on liquid storage pot (4), be equipped with ooff valve (6) on drain pipe (42), be equipped with mounting groove (31) on carousel (3), be equipped with test tube (7) in mounting groove (31), test tube (7) are located drain pipe (42) one side, a serial communication port, workstation (2) one side is even there is lifter (8), lifter (8) one end is even there is telescopic link (9), telescopic link (9) one end is clamping ring (10), be equipped with funnel (11) in clamping ring (10), between drain pipe (42) and test tube (7) are located to funnel (11).

2. A cell culture medium dispensing device according to claim 1, wherein the clamping ring (10) comprises two oppositely arranged arc-shaped rings, and the funnel (11) is internally provided with a filter membrane.

3. A cell culture medium dispensing device according to claim 1, wherein the number of mounting grooves (31) and test tubes (7) are several, both being evenly spaced apart in the circumferential direction of the turntable (3).

4. A cell culture medium split charging device according to claim 3, wherein one side of the turntable (3) is connected with a middle rotating shaft (32), the turntable (3) is rotatably connected to the base (1) through the middle rotating shaft (32), gear teeth (33) are arranged on the turntable (3), a driving device (12) is arranged on the base (1), the driving device (12) is cooperatively connected with the gear teeth (33), and the driving device (12) drives the gear teeth (33) to drive the turntable (3) to intermittently rotate.

5. The cell culture medium split charging device according to claim 4, further comprising a master control system, wherein the master control system is respectively connected with the stirring device (5), the switching valve (6), the lifting rod (8), the telescopic rod (9) and the driving device (12).

6. The cell culture medium split charging device according to claim 5, wherein the transit shaft (32) is hollow and tubular, the sensor (13) is arranged in the mounting groove (31), the sensor (13) is connected with the master control system, and the test tube (7) is arranged on the sensor (13).

7. The cell culture medium split charging device according to claim 5, wherein a liquid level switch is arranged in the mounting groove (31), the liquid level switch is arranged at 1/4 of the height of the test tube (7), and the liquid level switch is connected with a master control system.

8. The cell culture medium split charging device according to claim 5, wherein the liquid storage tank (4) is provided with a partition layer, an additive inlet (43) and a liquid level meter, the partition layer is provided with a temperature-adjusting liquid inlet (44) and a temperature-adjusting liquid outlet (45), and the liquid level meter is connected with a general control system.

9. The cell culture medium split charging device according to claim 5, wherein the liquid outlet pipe (42) is provided with a micro-pump (14), the micro-pump (14) is connected with a master control system, the micro-pump (14) is arranged at one side of the switch valve (6), and a filter screen is arranged in the liquid outlet pipe (42).

10. A cell culture medium dispensing device according to claim 6, wherein the sensor (13) is a weight sensor.