CN115595251A

CN115595251A - Culture dish filling device and using method

Info

Publication number: CN115595251A
Application number: CN202211137390.8A
Authority: CN
Inventors: 刘辉; 陈潇; 刘岚铮; 时玉雯
Original assignee: Jinan Municipal Center For Disease Control & Prevention
Current assignee: Jinan Municipal Center For Disease Control & Prevention
Priority date: 2022-09-19
Filing date: 2022-09-19
Publication date: 2023-01-13

Abstract

The invention discloses a culture dish filling device and a using method, relates to the technical field of microbial culture, solves the problems of complex operation, high use cost and high pollution risk of the existing culture medium filling mode, reduces the operation difficulty and the pollution risk, and adopts the following specific scheme: the filling module is composed of a driving unit and a supporting block connected with the driving unit through a telescopic rod, the filling bag is connected with the supporting block in a hanging mode, a rotating portion is arranged on the supporting block and driven by a rotating motor inside the supporting block, a rotary switch is arranged on a filling head at the bottom of the filling bag, and the rotary switch is connected to the rotating portion in a clamping mode.

Description

Culture dish filling device and using method

Technical Field

The invention relates to the technical field of microbial culture, in particular to a culture dish filling device and a using method thereof.

Background

The statements herein merely provide background information related to the present disclosure and may not necessarily constitute prior art.

In the microbial culture, the culture medium is filled into a culture dish as an indispensable process, and the currently commonly used filling mode is that the culture medium is taken out from a glass bottle contained in the culture dish through a conduit pipe under the action of a peristaltic pump and is filled into the culture dish.

The inventors have found that, in order to avoid the risk of contamination, existing filling methods require cleaning and autoclaving of the catheter before each filling, which greatly increases the workload; the glass bottle for containing the culture medium is of a special structure, the manufacturing cost is high, the glass bottle needs to be reused for reducing the use cost, and when the glass bottle is reused, the glass bottle needs to be cleaned and sterilized before the culture medium is contained every time, so that the workload is further increased;

the inventor also finds that the guide tube and the glass bottle not only need to ensure the effectiveness of cleaning and sterilization each time, but also need to avoid the pollution at the connection part of the bottle mouth and the guide tube when the guide tube and the glass bottle are used in a matching way, otherwise, the pollution of all culture mediums in the bottle is easily caused.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide a culture dish filling device and a using method thereof.

In order to achieve the purpose, the invention is realized by the following technical scheme:

in a first aspect, the invention provides a culture dish filling device, which comprises a filling module and a filling bag hung on the filling module, wherein the filling module is composed of a driving unit and a supporting block connected with the driving unit through a telescopic rod, the filling bag is hung on the supporting block, a rotating part is arranged on the supporting block, the rotating part is driven by a rotating motor in the supporting block, a filling head at the bottom of the filling bag is provided with a rotating switch, and the rotating switch is clamped on the rotating part.

As a further implementation manner, a first support frame is vertically and fixedly arranged at the top of the support block, a second support frame is horizontally and fixedly arranged at the side of the support block, a hanging rod is fixedly arranged on the first support frame, the top of the filling bag is hung on the hanging rod, and the filling head penetrates through the second support frame.

As a further implementation mode, the first support frame is of a telescopic structure, and the second support frame is located below the hanging rod.

As a further implementation manner, the second support frame is provided with a U-shaped through hole, the filling head penetrates through the U-shaped through hole, a limiting groove is arranged below the U-shaped through hole, and the limiting groove is matched with a limiting plate on the filling head to limit the position of the filling head.

As a further implementation manner, the second support frame is positioned above the rotating part.

As a further implementation manner, the rotating portion is composed of two arc-shaped stoppers and a fixing plate, the two arc-shaped stoppers are relatively fixedly arranged on the fixing plate, and the fixing plate is fixedly connected with the rotating shaft of the rotating motor.

As a further implementation manner, the rotary switch is rotatably disposed on the filling head, the rotary switch is composed of a handle, a valve rod and a sphere which are sequentially connected, the sphere is located in the filling head, the outer diameter of the sphere is the same as the inner diameter of the filling head, a through hole is formed in the sphere, and the handle is clamped on the rotary portion.

As a further implementation mode, the side part of the driving unit is fixedly provided with an injection supporting table, the injection supporting table is positioned below the supporting block, the top of the injection supporting table is provided with a weighing table, and the weighing table is connected with a weight sensor inside the injection supporting table through a weighing rod.

As a further implementation manner, a PLC control mechanism and a position sensor are further provided on the supporting block, and the position sensor, the weight sensor, the driving unit and the rotating motor are all connected with the PLC control mechanism.

In a second aspect, the present invention provides a method for using a culture dish filling system, which comprises the following steps:

the filling bag is hung on the filling module, so that the filling head penetrates through the second support frame, the limiting plate is positioned right below the limiting groove, and the handle is clamped on the rotating part;

adjusting the length of the first support frame to enable the limiting plate to be tightly clamped with the limiting groove;

placing the culture dish on a weighing platform;

the PLC control mechanism controls the driving unit to work according to the received position signal, moves the supporting block to the position right above the culture dish to be filled, drives the rotating motor to work after the supporting block is moved in place, and turns on the rotating switch through the rotating part;

when the culture dish is filled to the set weight, the PLC control mechanism controls the rotating part to rotate to close the rotary switch, and the filled culture dish is taken away while the other culture dish to be filled is placed on the weighing platform;

and repeating the filling action until the filling work of all the culture dishes is finished.

The beneficial effects of the invention are as follows:

(1) The culture medium is placed in the filling bag, the use of a glass bottle is omitted, the use cost is reduced, the filling bag is not required to be reused, the cleaning and sterilizing process is omitted, meanwhile, the filling head of the filling bag is provided with the rotary switch which can be opened or closed through the filling module, the culture medium can flow into a culture dish under the action of gravity, the use of a conduit and a peristaltic pump is omitted, and the pollution problem of the culture medium is effectively avoided.

(2) The first support frame is of a telescopic structure, the height of the first support frame can be changed according to the length of an actual filling bag, and the adaptability of the filling module is greatly improved.

(3) The limiting plate can be clamped in the limiting groove, so that the shaking of the filling head can be avoided, the rotation of the filling head in the horizontal plane can be prevented, meanwhile, the first supporting frame is of a telescopic structure, the force can be applied to the limiting plate through the pulling bag body, the limiting plate can be fully clamped in the limiting groove and cannot slide out in use, and the reliability of the filling process is greatly improved.

(4) The culture dish filling device is provided with the sensors, and the PLC control mechanism can control the filling system to automatically fill culture mediums into different culture dishes according to information monitored by the sensors, so that the filling accuracy is ensured, and the labor intensity is reduced.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.

FIG. 1 is a schematic diagram of the overall construction of a culture dish filling apparatus according to one or more embodiments of the present invention;

FIG. 2 is a schematic diagram of a side view of a culture dish filling apparatus according to one or more embodiments of the present invention;

FIG. 3 is a schematic structural view of a priming module according to one or more embodiments of the present invention (first support frame not shown);

FIG. 4 is a schematic diagram of a refill bag according to one or more embodiments of the present invention;

FIG. 5 is a schematic view of a rotary portion according to one or more embodiments of the present disclosure;

in the figure: the mutual spacing or size is exaggerated to show the position of each part, and the schematic diagram is only used for illustration;

wherein, 1, filling module; 2. filling a bag; 3. a drive unit; 4. a telescopic rod; 5. a supporting block; 6. a first support frame; 7. a second support frame; 8. a rotating part; 9. a bag body; 10. a filling head; 11. a rotary switch; 12. a limiting plate; 13. a limiting groove.

Detailed Description

It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the invention as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

As described in the background, existing filling methods require cleaning and autoclaving of the catheter prior to each filling, which greatly increases the workload; and the glass bottle that holds the culture medium is special structure, and the cost of manufacture is high, needs used repeatedly for reducing use cost, and need wash, disinfect to the glass bottle before holding the culture medium at every turn when used repeatedly, and this has further increaseed work burden. The invention provides a culture dish filling device and a use method thereof, aiming at solving the technical problems that a guide pipe and a glass bottle need to ensure the effectiveness of cleaning and sterilizing each time, and the pollution of a bottle mouth and the connection part of the guide pipe is avoided when the guide pipe and the glass bottle are used in a matched mode, otherwise, all culture mediums in the bottle are easy to pollute.

Example 1

In an exemplary embodiment of the present invention, as shown in fig. 1-5, a culture dish filling apparatus is provided, which includes a filling module 1 and a filling bag 2, wherein the filling bag 2 contains a culture medium, the filling bag 2 is placed on the filling module 1, and the culture medium in the filling bag 2 is filled into a culture dish at a designated position through the filling module 1.

As shown in fig. 2-3, the filling module 1 comprises a driving unit 3 and a supporting block 5, the driving unit 3 is connected to the supporting block 5 through an expansion link 4, wherein the driving unit 3 is a telescopic cylinder structure, and can control the expansion and contraction of the expansion link 4 to change the position of the supporting block 5.

The first support frame 6 and the second support frame 7 are arranged on the support block 5, wherein the first support frame 6 is fixedly arranged at the top of the support block 5, the second support frame 7 is fixedly arranged at the side part of the support block 5, and the filling bag 2 can be fixedly arranged on the support block 5 through the first support frame 6 and the second support frame 7, so that the position of the filling bag 2 can be changed along with the movement of the support block 5 to adapt to the filling requirements of culture dishes at different positions.

The first support frame 6 is vertically arranged, a hanging rod is arranged on the first support frame 6, and the filling bag 2 can be hung on the hanging rod so as to fix the position of the filling bag 2 on the support block 5.

In order to ensure that the filling module 1 can adapt to filling bags 2 with different lengths, the first support frame 6 is arranged to be of a telescopic structure, the height of the first support frame 6 can be changed according to the length of the actual filling bag 2, and the adaptability of the filling module 1 is greatly improved.

It is understood that the first supporting frame 6 may be a frame supporting structure formed by a plurality of steel pipes, or may be a plate-shaped supporting structure made of steel plates, the specific structure type may be determined according to actual design requirements, the telescopic structure form is the prior art, and the specific structure is not limited too much here.

The second support frame 7 is horizontally arranged on the side part of the support block 5, the second support frame 7 is positioned below the hanging rod, a U-shaped through hole is formed in the second support frame 7, and the filling head 10 on the filling bag 2 can pass through the U-shaped through hole of the second support frame 7;

the second support frame 7 is also provided with a limiting groove 13, and the limiting groove 13 is positioned below the U-shaped through hole and is mainly used for limiting the position of the filling head 10 on the filling bag 2 and preventing the filling head 10 from rotating when the filling head 10 is opened.

The supporting block 5 is further provided with a rotating part 8, the rotating part 8 is located on the side part of the supporting block 5 and is located on the same side as the second supporting frame 7, and the rotating part 8 is located below the second supporting frame 7 and is mainly used for opening/closing the filling head 10.

Rotating part 8 comprises two arc dogs and a fixed plate, two arc dog relatively fixed set up on the fixed plate, contactless between two arc dogs, be equipped with the mounting hole on the supporting shoe 5, the fixed plate rotates and sets up in the mounting hole of supporting shoe 5, the arc dog outwards stretches out from the mounting hole, be equipped with rotating electrical machines in the supporting shoe 5, rotating electrical machines's rotation axis and fixed plate fixed connection to can drive rotating part 8 wholly and take place to rotate for supporting shoe 5.

As shown in fig. 4, the filling bag 2 is composed of a bag body 9, a filling head 10, a rotary switch 11 and a limiting plate 12, the bag body 9 is made of transparent plastic and is mainly used for containing a culture medium, a rim is fixedly arranged on the outer side of the bag body 9 along the circumferential direction of the bag body, a plurality of hanging holes are formed in the rim at the top of the bag body 9, and a hanging rod on the first support frame 6 can pass through the hanging holes, so that the filling bag 2 is hung on the first support frame 6.

Wherein, the quantity of peg is no less than to the quantity of hitching hole, and the width direction interval of hitching hole edge bag body 9 sets up, and all hitching holes are in on the same horizontal plane to guarantee the stability that filling bag 2 articulates.

The filling head 10 is of a tubular structure, the filling head 10 is fixedly arranged at the bottom of the bag body 9, the filling head 10 is communicated with the inside of the bag body 9, a culture medium in the bag body 9 can be filled into a culture dish through the filling head 10, the filling head 10 is made of a plastic material, the filling head can be used at one time, and can also be reused after cleaning and sterilization, optimally, the filling head 10 is used at one time, the bag body 9 and the filling head 10 are made of plastics, the use cost of the filling bag 2 is greatly reduced, the repeated use is not needed, the cleaning and sterilization burdens are greatly reduced, and the pollution risk of the repeated use is reduced.

It can be understood that the filling head 10 can be fixed to the bag body 9 by means of bonding or the like, and can also be in threaded connection, when threaded connection is adopted, an external thread needs to be formed on the outer side wall of the top of the filling head 10, meanwhile, an installation seat with an internal thread is fixedly arranged at the bottom of the bag body 9, and fixed connection is achieved through threaded fit, so that the filling head 10 can be repeatedly used, and the specific connection mode can be selected according to actual design requirements without excessive limitation.

The rotary switch 11 is rotatably arranged on the filling head 10 and used for controlling the on-off of the filling head 10, the rotary switch 11 is composed of a handle, a valve rod and a sphere, wherein the sphere is positioned inside the pipe body of the filling head 10, the outer diameter of the sphere is the same as the inner diameter of the pipe body of the filling head 10, a through hole is formed in the sphere, when the axis of the through hole is coaxial with the axis of the pipe body of the filling head 10, the culture medium is allowed to be discharged from the filling head 10, and when the axis of the through hole is perpendicular to the axis of the pipe body of the filling head 10, the culture medium is prevented from flowing out.

The spheroid passes through the valve rod and is connected with the handle that is located filling head 10 outside, the middle part of handle is circular shape installation department, be provided with two convex joint portions relatively along its diametric (al) on the installation department, the diameter of installation department is not more than the synthetic circular shape diameter that two arc dogs of rotating part 8 enclose, but joint portion joint is between the adjacent tip of two arc dogs, thereby can drive the handle when rotating 8 emergence of rotating part rotates, and then drive the spheroid rotation in the filling head 10, in order to realize filling head 10's break-make.

The limiting plate 12 is fixedly arranged on the filling head 10, the limiting plate 12 is located above the rotary switch 11, and the limiting plate 12 is mainly used for limiting the position of the filling head 10, preventing the filling head 10 from deviating or rotating when the rotary switch 11 rotates, and avoiding the influence on the operation of the rotary switch 11.

The shape of the limiting plate 12 is the same as that of the limiting groove 13, the limiting plate 12 is of an irregular structure, and the limiting plate 12 can be clamped in the limiting groove 13, so that the filling head 10 can be prevented from shaking and the filling head 10 can be prevented from rotating in the horizontal plane.

Because first support frame 6 is the telescopic structure, the accessible is carried and is drawn the bag body 9 and carry out the application of force to limiting plate 12 to guarantee that limiting plate 12 can be abundant the joint in spacing groove 13 and can not the in use roll-off, improved the reliability of filling process greatly.

The use of the filling bag 2 cancels a peristaltic pump and a conduit, avoids the cleaning and sterilizing operations of the conduit and other structures, reduces the working strength, avoids the pollution problems caused by incomplete cleaning and sterilization and the connection of the conduit and the culture medium containing device, facilitates the transportation and storage of the culture medium, and reduces the use cost.

In order to facilitate the precise control of the culture dish filling, a filling support platform is further fixedly arranged on the side of the driving unit 3, and the filling support platform is located below the support block 5, specifically, right below the second support frame 7.

The inside of filling brace table is equipped with weighing sensor, the top of filling brace table is equipped with weighing platform, weighing platform's top is equipped with the recess that is used for holding the culture dish, weighing platform's bottom is connected with weighing in the platform weighing sensor through weighing pole to when weighing bench culture dish filling, the accessible weighing pole gives gravity sensor with gravity transfer, and then the gravity change through gravity sensor monitoring culture dish, with the filling volume of accurate control culture medium.

The automatic culture medium filling device is characterized by further comprising a PLC control mechanism and a position sensor, wherein rotating motors in the position sensor, the gravity sensor, the driving unit 3 and the supporting block 5 are all connected with the remote PLC control mechanism, and the PLC control mechanism can control the filling system to automatically fill culture media into different culture dishes according to information monitored by the position sensor and the weight sensor.

Wherein, PLC control mechanism and position sensor all set up on supporting shoe 5, and position sensor's setting can be convenient for when monitoring the culture dish position, and monitoring supporting shoe 5's position avoids supporting shoe 5 to remove the position and lead to the filling to appear the deviation.

Through the setting of position sensor, weighing transducer, PLC control mechanism can monitor the position of culture dish and the filling weight of culture medium in the culture dish to guarantee the automation and the degree of accuracy of filling work.

It can be understood that the PLC control mechanism in this embodiment is an existing PLC controller, and the specific structure is not described here in detail.

Example 2

In another exemplary embodiment of the present invention, a method of using the culture dish filling apparatus described in example 2 is provided, as follows:

firstly, placing the filling bag 2 on the filling module 1, so that the hanging rod on the first support frame 6 penetrates through the hanging hole at the top of the filling bag 2;

a filling head 10 at the bottom of the filling bag 2 penetrates through the second support frame 7, so that the limiting plate 12 is positioned right below the limiting groove 13, and meanwhile, a handle on the rotary switch 11 is clamped on the rotating part 8;

the length of the first support frame 6 is adjusted according to the connection strength of the limiting plate 12 and the limiting groove 13 until the limiting plate 12 is tightly clamped with the limiting groove 13;

placing the culture dish in the groove on the weighing platform;

the PLC control mechanism receives a monitoring signal transmitted by the position sensor, controls the telescopic cylinder to work according to the monitoring signal, moves the supporting block 5 to the position right above the culture dish to be filled through the telescopic rod 4, drives the rotating motor to work after the supporting block is moved in place, and turns on the rotating switch 11 through the rotating part 8 so as to fill the culture medium into the culture dish;

when the weight is filled to the set weight, the weight sensor transmits a weight signal to the PLC control mechanism, the PLC control mechanism controls the rotating part 8 to rotate to close the rotary switch 11, the telescopic rod 4 works to withdraw the supporting block 5, the filled culture dish is taken away, and the other culture dish to be filled is placed on the weighing platform;

It can be understood that if the culture medium in the filling bag 2 runs out during the filling process, the filling bag 2 can be replaced manually at any time without affecting the filling accuracy.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The culture dish filling device is characterized by comprising a filling module and a filling bag hung on the filling module, wherein the filling module is composed of a driving unit and a supporting block connected with the driving unit through a telescopic rod, the filling bag is hung on the supporting block, a rotating part is arranged on the supporting block, the rotating part is driven by a rotating motor inside the supporting block, a filling head at the bottom of the filling bag is provided with a rotating switch, and the rotating switch is clamped on the rotating part.

2. The culture dish filling device according to claim 1, wherein a first support frame is vertically fixed on the top of the support block, a second support frame is horizontally fixed on the side of the support block, a hanging rod is fixed on the first support frame, the top of the filling bag is hung on the hanging rod, and the filling head passes through the second support frame.

3. A culture dish filling apparatus as claimed in claim 2, wherein the first support frame is a telescopic structure and the second support frame is located below the hanging rod.

4. The culture dish filling device according to claim 2, wherein the second support frame is provided with a U-shaped through hole, the filling head penetrates through the U-shaped through hole, a limiting groove is arranged below the U-shaped through hole, and the limiting groove is matched with a limiting plate on the filling head to limit the position of the filling head.

5. A petri dish filling device according to claim 2, wherein the second support frame is located above the rotating portion.

6. A culture dish filling apparatus according to claim 1, wherein the rotating part comprises two arc-shaped stoppers and a fixing plate, the two arc-shaped stoppers are relatively fixedly arranged on the fixing plate, and the fixing plate is fixedly connected to the rotating shaft of the rotating motor.

7. The culture dish filling device according to claim 1, wherein the rotary switch is rotatably arranged on the filling head, the rotary switch is composed of a handle, a valve rod and a sphere which are sequentially connected, the sphere is positioned in the filling head, the outer diameter of the sphere is the same as the inner diameter of the filling head, a through hole is formed in the sphere, and the handle is clamped on the rotating part.

8. The culture dish filling device according to claim 1, wherein a filling support platform is fixedly arranged at the side of the driving unit, the filling support platform is positioned below the support block, a weighing platform is arranged at the top of the filling support platform, and the weighing platform is connected with a weight sensor inside the filling support platform through a weighing rod.

9. A culture dish filling device according to claim 8, wherein the support block is further provided with a PLC control mechanism and a position sensor, and the position sensor, the weight sensor, the driving unit and the rotating motor are all connected with the PLC control mechanism.

10. A method of using a culture dish filling apparatus as claimed in any one of claims 1 to 9, in particular as follows:

the filling bag is hung on the filling module, so that the filling head passes through the second support frame, the limiting plate is positioned right below the limiting groove, and the handle is clamped on the rotating part;

placing the culture dish on a weighing platform;

when the culture dish to be filled is filled to a set weight, the PLC control mechanism controls the rotating part to rotate to close the rotary switch, and the filled culture dish is taken away while another culture dish to be filled is placed on the weighing platform;