CN220323124U - Fluorescence detection device based on gene editing technology - Google Patents
Fluorescence detection device based on gene editing technology Download PDFInfo
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- CN220323124U CN220323124U CN202321992103.1U CN202321992103U CN220323124U CN 220323124 U CN220323124 U CN 220323124U CN 202321992103 U CN202321992103 U CN 202321992103U CN 220323124 U CN220323124 U CN 220323124U
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- detection box
- fixedly connected
- device based
- detection device
- gene editing
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- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 18
- 238000010362 genome editing Methods 0.000 title claims abstract description 18
- 238000005516 engineering process Methods 0.000 title abstract description 9
- 238000001514 detection method Methods 0.000 claims abstract description 41
- 239000011521 glass Substances 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 9
- 238000012360 testing method Methods 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 5
- 108060001084 Luciferase Proteins 0.000 description 4
- 239000005089 Luciferase Substances 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 1
- 108090000331 Firefly luciferases Proteins 0.000 description 1
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 1
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 1
- 108700008625 Reporter Genes Proteins 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000010794 food waste Substances 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a fluorescence detection device based on a gene editing technology, which comprises a detection box and a placement frame, wherein the detection box is made of transparent glass, the placement frame is fixedly connected above the right side of the inner wall of the detection box, a placement hole is formed in the placement frame, a supporting plate is fixedly connected to the right side of the inner wall of the detection box and below the placement frame, a telescopic rod is fixedly connected to the right side of the inner wall of the detection box and below the supporting plate, and a supporting block is fixedly connected to the telescopic end of the telescopic rod. According to the utility model, through the mutual matching of the detection box, the placement frame, the placement hole, the support supporting plate, the telescopic rod, the support block, the light source emitter, the movable port, the fixed plate, the clamping groove, the threaded hole and the threaded rod, the problems that the common fluorescent detection device based on the gene editing technology is poor in use effect, the distance between the light source and the test tube cannot be adjusted according to actual conditions, and the practicability of the fluorescent detection device is greatly reduced are solved.
Description
Technical Field
The utility model relates to the technical field of gene editing, in particular to a fluorescence detection device based on a gene editing technology.
Background
The fluorescence detection is a natural luminescence reaction, the luciferase reacts with ATP, the luciferase reporter gene is a reporter system for detecting firefly luciferase activity by using a luciferin substrate, the luciferase can catalyze and oxidize, and biological fluorescence can be emitted in the catalyzing and oxidizing process, so that the luciferase can be used for detecting human cells, genes, bacteria, mold and food residues, and a reaction result can be obtained within 15 seconds.
At present, the common fluorescence detection device based on the gene editing technology is poor in using effect, the distance between a light source and a test tube cannot be adjusted according to actual conditions, and the practicability of the fluorescence detection device is greatly reduced.
Disclosure of Invention
The present utility model is directed to a fluorescence detection device based on gene editing technology, so as to solve the problems set forth in the background art.
In order to achieve the above purpose, the present utility model provides the following technical solutions: a fluorescence detection device based on a gene editing technique, comprising:
a detection box;
the upper right side of the inner wall of the detection box is fixedly connected with a placement frame, and a placement hole is formed in the placement frame;
the supporting plate is fixedly connected with the right side of the inner wall of the detection box and positioned below the placing frame;
the telescopic rod is fixedly connected with the right side of the inner wall of the detection box and positioned below the supporting plate, and the telescopic end of the telescopic rod is fixedly connected with the supporting block;
the top of the supporting block is fixedly connected with a light source emitter;
the movable opening is formed in the bottom of the detection box, and the bottom of the supporting block penetrates through the movable opening and extends to the outside of the movable opening.
Further, supporting legs are fixedly connected to four corners of the bottom of the detection box.
Further, the detection box bottom and be located the rear side fixedly connected with fixed plate of movable mouth, a plurality of draw-in grooves have been seted up in proper order from left to right to the front side of fixed plate.
Further, the screw hole has been seted up to the below of supporting shoe front side, the inside threaded connection of screw hole has the threaded rod, the rear end of threaded rod runs through screw hole and draw-in groove in proper order and extends to the inside of draw-in groove, movable joint between threaded rod and the draw-in groove, the front end of threaded rod runs through the screw hole and extends to the outside of screw hole and fixedly connected with handle.
Further, the top of the detection box is of an open structure.
Further, the supporting block is movably connected with the movable opening.
Further, the top of the detection box is movably clamped with a box cover.
Compared with the prior art, the utility model has the following beneficial effects:
according to the utility model, through the mutual matching of the detection box, the placement frame, the placement hole, the support supporting plate, the telescopic rod, the support block, the light source emitter, the movable port, the fixed plate, the clamping groove, the threaded hole and the threaded rod, the problems that the common fluorescent detection device based on the gene editing technology is poor in use effect, the distance between the light source and the test tube cannot be adjusted according to actual conditions, and the practicability of the fluorescent detection device is greatly reduced are solved.
Drawings
FIG. 1 is a schematic elevational view of the present utility model;
FIG. 2 is a cross-sectional view of the front structure of the present utility model;
FIG. 3 is a schematic side view of the present utility model;
fig. 4 is a schematic left-view structure of the present utility model.
In the figure: 1. a detection box; 2. a placing rack; 3. placing the hole; 4. a supporting pallet; 5. a telescopic rod; 6. a support block; 7. a light source emitter; 8. a movable opening; 9. a fixing plate; 10. a clamping groove; 11. a threaded hole; 12. a threaded rod.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present utility model, unless otherwise indicated, the meaning of "a plurality" is two or more.
In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.
Referring to fig. 1-4, a fluorescence detection device based on gene editing technology includes a detection box 1 and a placement frame 2, wherein the detection box 1 is made of transparent glass, the placement frame 2 is fixedly connected to the upper right side of the inner wall of the detection box 1, a placement hole 3 is formed in the placement frame 2, a supporting support plate 4 is fixedly connected to the right side of the inner wall of the detection box 1 and located below the placement frame 2, a telescopic rod 5 is fixedly connected to the right side of the inner wall of the detection box 1 and located below the supporting support plate 4, a supporting block 6 is fixedly connected to the telescopic end of the telescopic rod 5, a light source emitter 7 is fixedly connected to the top of the supporting block 6, a movable opening 8 is formed in the bottom of the detection box 1, and the bottom of the supporting block 6 penetrates through the movable opening 8 and extends to the outside of the movable opening 8.
Specifically, the bottom four corners department of detecting case 1 all fixedly connected with supporting leg.
In the concrete implementation, the bottom of the detection box 1 is fixedly connected with a fixing plate 9 at the rear side of the movable opening 8, and a plurality of clamping grooves 10 are sequentially formed in the front side of the fixing plate 9 from left to right.
Specifically, screw hole 11 has been seted up to the below of supporting shoe 6 front side, and the inside threaded connection of screw hole 11 has threaded rod 12, and threaded rod 12's rear end runs through screw hole 11 and draw-in groove 10 in proper order and extends to the inside of draw-in groove 10, and movable joint is between threaded rod 12 and the draw-in groove 10, and threaded rod 12's front end runs through threaded hole 11 and extends to the outside of screw hole 11 and fixedly connected with handle.
In the specific implementation, the top of the detection box 1 is of an open structure.
Specifically, the supporting block 6 is movably connected with the movable opening 8.
In the concrete implementation, the top of the detection box 1 is movably clamped with a box cover.
In addition, what is not described in detail in the present specification is all of the prior art known to those skilled in the art.
When in practical application: taking down the case lid, then insert the test tube that is equipped with gene and place in hole 3, and support the test tube bottom through supporting plate 4, then according to the demand, anticlockwise rotation handle, and then make threaded rod 12 move forward in screw hole 11, and then make threaded rod 12 break away from draw-in groove 10, then lateral shifting supporting shoe 6, and then drive light source transmitter 7 and carry out side-to-side motion, and then adjust the interval between light source transmitter 7 and the test tube to suitable size, then rotate the handle clockwise, and then make threaded rod 12 insert in corresponding draw-in groove 10, then cover the case lid, start light source transmitter 7, utilize light source transmitter 7 to transmit the light source, the light source shines on detecting the test tube, thereby can play the fluorescence detection effect that adds to the test tube, then observe through transparent glass and examine the inside condition of case 1 can.
All parts in the utility model are universal standard parts or parts known by a person skilled in the art, the structure and principle of the parts are known by the person skilled in the art through technical manuals or known by conventional experimental methods, and standard parts used in the application can be purchased from the market, all parts in the application can be customized according to the description of the specification and the drawings, the specific connection modes of all parts adopt conventional means such as mature bolts, rivets and welding in the prior art, the machinery, parts and equipment adopt conventional models in the prior art, the control mode of the utility model is automatically controlled by a controller, the control circuit of the controller can be realized through simple programming by the person skilled in the art, the supply of power also belongs to common general knowledge in the art, and the utility model is mainly used for protecting mechanical devices, so the utility model does not explain the control mode and circuit connection in detail.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A fluorescence detection device based on a gene editing technique, comprising:
a detection box (1), wherein the detection box (1) is made of transparent glass;
the device comprises a placing rack (2), wherein the placing rack (2) is fixedly connected to the upper right side of the inner wall of the detection box (1), and a placing hole (3) is formed in the placing rack (2);
the supporting plate (4) is fixedly connected with the supporting plate (4) on the right side of the inner wall of the detection box (1) and positioned below the placing frame (2);
the telescopic rod (5) is fixedly connected with the telescopic rod (5) on the right side of the inner wall of the detection box (1) and positioned below the supporting plate (4), and the telescopic end of the telescopic rod (5) is fixedly connected with the supporting block (6);
the light source emitter (7), the top of the supporting block (6) is fixedly connected with the light source emitter (7);
the movable opening (8) is formed in the bottom of the detection box (1), the movable opening (8) is formed in the bottom of the support block (6), and the bottom of the support block (6) penetrates through the movable opening (8) and extends to the outside of the movable opening (8).
2. The fluorescence detection device based on the gene editing technique according to claim 1, wherein: supporting legs are fixedly connected to four corners of the bottom of the detection box (1).
3. The fluorescence detection device based on the gene editing technique according to claim 1, wherein: the detection box is characterized in that a fixing plate (9) is fixedly connected to the bottom of the detection box (1) and located at the rear side of the movable opening (8), and a plurality of clamping grooves (10) are formed in the front side of the fixing plate (9) from left to right in sequence.
4. The fluorescence detection device based on the gene editing technique according to claim 1, wherein: screw hole (11) have been seted up to the below of supporting shoe (6) front side, the inside threaded connection of screw hole (11) has threaded rod (12), the rear end of threaded rod (12) runs through screw hole (11) and draw-in groove (10) in proper order and extends to the inside of draw-in groove (10), movable joint between threaded rod (12) and draw-in groove (10), the front end of threaded rod (12) runs through screw hole (11) and extends to the outside of screw hole (11) and fixedly connected with handle.
5. The fluorescence detection device based on the gene editing technique according to claim 1, wherein: the top of the detection box (1) is of an open structure.
6. The fluorescence detection device based on the gene editing technique according to claim 1, wherein: the supporting block (6) is movably connected with the movable opening (8).
7. The fluorescence detection device based on the gene editing technique according to claim 1, wherein: the top of the detection box (1) is movably clamped with a box cover.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321992103.1U CN220323124U (en) | 2023-07-27 | 2023-07-27 | Fluorescence detection device based on gene editing technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321992103.1U CN220323124U (en) | 2023-07-27 | 2023-07-27 | Fluorescence detection device based on gene editing technology |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220323124U true CN220323124U (en) | 2024-01-09 |
Family
ID=89416654
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321992103.1U Active CN220323124U (en) | 2023-07-27 | 2023-07-27 | Fluorescence detection device based on gene editing technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220323124U (en) |
-
2023
- 2023-07-27 CN CN202321992103.1U patent/CN220323124U/en active Active
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