CN220317776U

CN220317776U - Aseptic culture box

Info

Publication number: CN220317776U
Application number: CN202321849337.0U
Authority: CN
Inventors: 钱杨华; 任清华
Original assignee: Shanghai Jinyu Biotechnology Co ltd
Current assignee: Shanghai Jinyu Biotechnology Co ltd
Priority date: 2023-07-14
Filing date: 2023-07-14
Publication date: 2024-01-09
Anticipated expiration: 2033-07-14

Abstract

The utility model relates to the field of cell culture flasks, in particular to a sterile culture box, which comprises a culture box shell, a sealing cover and a culture box front door. The upper part and the lower part of the culture box shell are provided with air vents, the inside of each air vent is provided with a ventilated membrane, the bottom of each air vent is provided with a footing, the side surface is close to the front door, a door lock fixing block is arranged at the position, and support plates are arranged at the left and right bottom sides inside the shell; a sealing ring is arranged below the sealing cover; the culture box front door is provided with a handle in the middle, a door lock is arranged on the left and the right, a door sealing ring is arranged on the periphery of the door, a tray is connected inside the door through bolts, and a culture container is placed on the tray. In the utility model, in the aseptic culture box, a non-closed common container culture container can be used, and the aseptic closed culture can be realized by matching with a common incubator; meanwhile, the device can be matched with an aseptic isolator to realize aseptic protection of the whole process of cell and microorganism culture and production operation.

Description

Aseptic culture box

Technical Field

The utility model relates to a sterile culture box for culturing cells and microorganisms, and belongs to the technical field of culture.

Background

In the existing culturing process of cells or microorganisms by adopting a non-closed system and a sterile isolator, if the sterility of the whole culturing process is to be realized, the existing scheme generally adopts a mode of integrating an incubator matched with the isolator or an external incubator which can be in sterile butt joint. When the cell or microorganism is actually cultured, the cell or microorganism is prepared and treated before the culture, the liquid is changed during the observation in the culture process, the harvest, the preparation and other operations after the culture are carried out in the isolator, and then the culture process is put in the matched incubator. In order to ensure the sterility of the transfer process of the culture container between the incubator and the sterile isolator, the first mode is to use a fixed incubator integrated with the sterile isolator, and the door of the incubator is directly opened in the sterile isolator, so that the culture container can be directly transferred in the cavities of the sterile isolator and the incubator without being exposed in other environments, and the sterility of the whole process can be realized. The second way is to use an external honeycomb incubator, which can be aseptically docked with the sterile isolator, and then the culture vessel is aseptically transferred between the incubator and the sterile isolator. Both of these current approaches have two disadvantages:

1) The cost of a custom made special incubator, whether an integrated incubator or a honeycomb incubator, is relatively high, and the size of the incubator is limited for interfacing with the isolator, with fewer alternative specifications.

2) The transfer of the incubator switch door or the incubator needs to be operated through the glove of the sterile isolator no matter the incubator is an integrated incubator or a honeycomb incubator, and the integrated incubator and the honeycomb incubator are generally arranged on the side surface or the back surface of the isolator, are far away from the glove, and have larger operation difficulty.

Disclosure of Invention

The utility model aims to solve the defects in the prior art and provides a sterile culture box for culturing cells and microorganisms.

In order to achieve the above purpose, the present utility model adopts the following technical scheme:

an aseptic culture box for culturing cells and microorganisms comprises a culture box shell, a sealing cover and a culture box front door.

The culture box shell is provided with a vent, the vent is internally provided with a ventilated membrane, the bottom is provided with a footing, the side surface is close to the front door, a door lock fixing block is arranged at the position, and support plates are arranged at the left and right bottom sides inside the shell;

a sealing ring is arranged below the sealing cover;

the culture box comprises a culture box front door, a handle, door locks, a door sealing ring, a tray, a culture container, a culture box cover and a culture box cover, wherein the handle is arranged in the middle of the culture box front door;

preferably, the air-permeable membrane is made of a material capable of blocking microorganisms, and can isolate outside microorganisms from entering the culture box shell during culture.

Preferably, the vent of the culture cassette housing is sealed by a sealing cap and a sealing ring, if necessary.

Preferably, the front opening of the shell of the incubator can be sealed by the front door and the door sealing ring of the incubator, and then the front door is locked by the door lock and the door lock fixing block.

Preferably, the tray can be supported on the supporting plate and can be pushed in and pulled out together with the front door of the culture box, so that the culture container placed on the tray can be conveniently taken and placed.

The beneficial effects of the utility model are as follows:

1. in the utility model, the sterile culture box is a closed system, and during culture, the sterile closed culture can be realized by only adopting a common culture container and placing the common culture container in a common culture box.

2. The utility model can seal the sealing cover and the front door together to form a completely closed container, when the culture container is required to be transferred into the sterile isolator, the sterile culture box can be firstly placed into a sterilization transmission window of the sterile isolator, the outer surface of the sterile culture box is sterilized and then transferred into the sterile isolator, the front door is opened in the isolator, the tray is pulled out, the culture container can be taken out for operation, after the operation is finished, the tray can be put back, the sterile culture box is pushed back, the isolator is discharged, the sealing cover is detached, and the sterile culture box is put back into the incubator for culture.

Drawings

FIG. 1 is an isometric view of the present utility model;

FIG. 2 is a cross-sectional view of the present utility model;

FIG. 3 is a schematic diagram of the composition of the present utility model;

fig. 4 is a schematic view of the front door assembly of the present utility model.

In the figure: 10. a culture cassette housing; 11. a vent; 12. a breathable film; 13. a support leg; 14. a door lock fixing block; 15. a support plate; 20. sealing cover; 21. a seal ring; 30. a front door of the culture box; 31. a handle; 32. a door lock; 33. a door seal ring; 34. a bolt; 35. a tray; 36. a culture vessel.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.

Referring to FIGS. 1-3, a sterile cassette includes a cassette housing 10, a seal cap 20, and a cassette front door 30.

The upper surface and the lower surface of the culture box shell 10 are provided with air vents 11, an air permeable membrane 12 is arranged in the air vents, feet 13 are arranged at the bottom, door lock fixing blocks 14 are arranged at positions of the side surfaces close to the front door, and support plates 15 are arranged at the left bottom side and the right bottom side of the shell;

a sealing ring 21 is arranged below the sealing cover 20;

the culture box front door 30 is provided with a handle 31 in the middle, door locks 32 are arranged on the left and right sides, door sealing rings 33 are arranged on the periphery of the door, a tray 35 is connected to the inside of the door through bolts 34, and a culture container 36 is placed on the tray.

Further, the ventilation membrane 12 is made of a material capable of filtering microorganisms, and can isolate outside microorganisms from entering the culture box shell during culture;

further, the vent 11 of the culture cassette housing 10 may be sealed by a sealing cover 20 and a sealing ring 21, if necessary;

further, the front opening of the incubator shell 10 can be sealed by the incubator front door 30 and the door sealing ring 33, and then the front door is locked by the door lock 32 and the door lock fixing block 14;

further, the tray 35 may be supported on the support plate 15 and may be pushed in and pulled out together with the cassette front door 30 to facilitate the taking and placing of the culture container 36 placed on the tray 35.

The implementation process of this embodiment is as follows:

before use, the sterile culture box is integrally sealed as shown in fig. 1, then is placed into the sterile isolator, and after being integrally sterilized together with the sterile isolator, the door lock 32 is released to pull the front door 30 and the tray 35 out, then the inoculated culture container 36 is placed on the tray 35, the tray is pushed back into the culture box shell 10, the door lock 32 is buckled, the front door 30 of the culture box is in a sealed state, and then the culture box can be transmitted out through a transmission window of the sterile isolator.

After the sterile culture cassette is transferred from the sterile isolator, the sealing cover 20 of the sterile culture cassette is removed, and then the sterile culture cassette is placed into an incubator to perform culture. During the culturing process, the sterile culturing cassette can be ventilated through the ventilation membrane 21 to meet the gas requirements for cell culture in the culturing container 36 in the sterile culturing cassette, while maintaining sterility in the culturing cassette housing 10.

When it is necessary to transfer the culture container 36 into the aseptic isolator, the vent 11 of the culture cassette housing 10 is first covered with the sealing cover 13 and the middle is sealed with the sealing ring. Then, the aseptic culture box is placed into a sterilization transmission window of an aseptic isolator, the outer surface of the aseptic culture box is sterilized, then the aseptic culture box is transferred into the aseptic isolator, the door lock 32 is released, the tray 35 is pulled out, and the culture container 36 is taken out, so that operations such as cell observation, sampling, liquid replacement, centrifugation, harvesting, preparation and the like can be performed.

Thereafter, the cultivation is also required as described above, and the previous operation is repeated, and the sterile cultivation cassette is returned to the incubator for the operation. If the sterile culture box does not need to be cultured, the sterile culture box can be taken out, the sealing cover 20, the sealing ring 21 and the front door 30 can be pulled out, the interior of the sterile culture box is cleaned, and the sterile culture box is taken out integrally for later use.

The foregoing is only a preferred embodiment of the present utility model, but the scope of the present utility model is not limited thereto, and any person skilled in the art, who is within the scope of the present utility model, should make equivalent substitutions or modifications according to the technical scheme of the present utility model and the inventive concept thereof, and should be covered by the scope of the present utility model.

Claims

1. The aseptic culture box comprises a culture box shell, a sealing cover and a culture box front door, and is characterized in that a vent is arranged on the culture box shell, a breathable film is arranged in the vent, feet are arranged at the bottom, door lock fixing blocks are arranged at positions, close to the front door, of the side surfaces of the bottom, and support plates are arranged at the left bottom side and the right bottom side of the interior of the shell;

the inside of the front door of the culture box is connected with a tray, and a culture container is placed on the tray.

2. The sterile incubator of claim 1, wherein the vent is internally mounted with a gas permeable membrane made of a material that can block microorganisms.

3. The sterile incubator of claim 1, wherein the vents are provided at the upper and lower sides or at the left and right sides.

4. The sterile culture cassette of claim 1, wherein the number of vents is two or more.

5. A sterile incubator according to claim 1 wherein the seal is provided with a seal and the vent of the incubator housing is sealable by the seal and the seal.

6. The sterile cultivation box according to claim 1, wherein a handle is arranged in the middle of the front door of the cultivation box, a door lock is arranged at the left and right sides, a door sealing ring is arranged at one circle of the door, the front opening of the cultivation box shell can be sealed through the front door of the cultivation box and the door sealing ring, and then the front door is locked through the door lock and the door lock fixing block.

7. The sterile incubator of claim 1, wherein the incubator housing door lock blocks are provided on the left and right sides, the upper and lower sides, or the upper and lower sides, the left and right sides, and the door lock of the incubator front door is provided on the left and right sides, the upper and lower sides, or the upper and lower sides, the left and right sides.

8. A sterile incubator according to claim 1 wherein the tray is supportable on the support plate and is retractable in and out with the incubator front door.