CN111500446A - Biological safety cabinet and CO2Integrated fertilized egg preparation equipment of incubator - Google Patents
Biological safety cabinet and CO2Integrated fertilized egg preparation equipment of incubator Download PDFInfo
- Publication number
- CN111500446A CN111500446A CN202010329241.6A CN202010329241A CN111500446A CN 111500446 A CN111500446 A CN 111500446A CN 202010329241 A CN202010329241 A CN 202010329241A CN 111500446 A CN111500446 A CN 111500446A
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- Prior art keywords
- incubator
- biological safety
- safety cabinet
- cabinet
- sealing door
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
Abstract
The invention provides a biological safety cabinet and CO2Integrated oosperm modulation equipment of incubator. The invention comprises the following steps: CO 22Incubator and biosafety cabinet, CO2CO is respectively arranged at the opposite sides of the incubator and the biological safety cabinet2Incubator outlet and biosafety cabinet inlet, CO2The output port of the incubator is connected with the input port of the biological safety cabinet through a flange, the biological safety cabinet is internally provided with a decontamination mechanism, and the input port of the biological safety cabinet is provided with a sealing door capable of being opened and closed2An incubator and a biological safety cabinet; when the fertilized eggs need to be cultured, the sealing door is closed, so that the cleanliness of the culture process is ensured; after the fertilized egg is cultured, it is removed from CO2The incubator is directly transferred to the biological safety cabinet, the whole process is closed, and the condition that the fertilized eggs are polluted cannot occur。
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a biological safety cabinet and CO2Integrated oosperm modulation equipment of incubator.
Background
The carbon dioxide incubator realizes the in-vitro culture of cells by simulating and forming a growth environment similar to that in an organism in the incubator body; when a laboratory worker operates experimental materials with infection, toxicity and volatility, the laboratory worker needs to operate in the biological safety cabinet, the biological safety cabinet can not only avoid the pollution of laboratory environment to the experimental materials, but also can ensure the physical health of the laboratory worker. Existing CO2The incubator and the biological safety cabinet are easy to be polluted when cells/tissues are transferred, the cell pollution can be mainly divided into physical, chemical and biological pollution according to pollution sources, and some pollution can even cause the nutrient loss of cell culture solution and the irreversible damage of eukaryotic cells.
Disclosure of Invention
In light of the above-mentioned technical problems, a biosafety cabinet and CO are provided2Integrated oosperm modulation equipment of incubator. The technical means adopted by the invention are as follows:
biological safety cabinet and CO2An integrated fertilized egg preparation apparatus of incubator comprising CO2Incubator and biosafety cabinet, said CO2CO is respectively arranged at the opposite sides of the incubator and the biological safety cabinet2Incubator outlet and biosafety cabinet inlet, CO2The output port of the incubator is connected with the input port of the biological safety cabinet through a flange, and the biological safety cabinet is internally provided with a dye removerThe input port of the biological safety cabinet is provided with a sealing door capable of being opened and closed;
in a dye removing state, the dye removing mechanism operates, and the sealing door is opened; under the condition that the fertilized eggs are cultured after the degainting is finished, the degainting mechanism stops running, and the sealing door is closed; after the fertilized eggs are cultured, the sealing door is opened, and the cultured fertilized eggs are cultured from CO2The incubator was transferred to a biosafety cabinet.
Further, it includes hydrogen peroxide generator to remove dyeing mechanism, the biological safety cabinet includes work area and air current mixed region, work area is equipped with the socket, and hydrogen peroxide generator is connected with the socket electricity, hydrogen peroxide generator includes hydrogen peroxide generating device and hydrogen peroxide decomposition device, air current mixed region is including breathing in fan and exhaust fan, and under the condition of removing dyeing, breathing in fan and exhaust fan are in the running state.
Further, the CO is2Sealing gaskets are arranged between the incubator and the biological safety cabinet and between the incubator and the flanges.
Further, the sealing door is hinged to the input port of the biological safety cabinet.
Furthermore, slide ways are arranged on the left side and the right side of the input port of the biological safety cabinet, and the sealing door can be arranged in the slide ways in a vertically sliding mode.
According to the invention, by opening the sealing door and opening the dye removing device, the dye removing airflow can be fully filled with CO2An incubator and a biological safety cabinet; when the fertilized eggs need to be cultured, the sealing door is closed, so that the cleanliness of the culture process is ensured; after the fertilized egg is cultured, it is removed from CO2The incubator directly transmits to the biological safety cabinet, and the whole process is closed, and the situation that the fertilized eggs are polluted can not occur.
For the above reasons, the present invention can be widely applied to the technical field of cell culture.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic structural diagram of embodiment 1 of the present invention.
In the figure: 1. CO 22An incubator; 2. a biological safety cabinet; 3. a flange; 4. a gasket; 5. a sealing door; 6. a hinge; 7. h2O2A generating device.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in FIG. 1, the embodiment of the invention discloses a biological safety cabinet and CO2Integrated zygote preparation equipment of incubator, comprising: CO 22Incubator 1 and biosafety cabinet 2, CO2CO is respectively arranged at the opposite sides of the incubator and the biological safety cabinet2Incubator outlet and biosafety cabinet inlet, CO2The output port of the incubator is connected with the input port of the biological safety cabinet through a flange 3, a decontamination mechanism is arranged in the biological safety cabinet, and the input port of the biological safety cabinet is provided with a sealing door 5 which can be opened and closed;
in a dye removing state, the dye removing mechanism operates, and the sealing door is opened; under the condition that the fertilized eggs are cultured after the degainting is finished, the degainting mechanism stops running, and the sealing door is closed; after the fertilized eggs are cultured, the sealing door is opened, and the cultured fertilized eggs are cultured from CO2The incubator was transferred to a biosafety cabinet.
The traditional biological safety cabinet adopts a formalin (formaldehyde aqueous solution) fumigation mode as a disinfection and decontamination mode, and is easy to carry out ring alignmentBorder and personnel cause pollution and injury, and the security is low, need seal the processing with all gaps of the cabinet body with sealing tape during this disinfection, then the cover is fumigated in big sealed bag and is removed the dyeing, and the work load is big and loaded down with trivial details inconvenience, even so still probably causes the injury to the personnel, so should wear the protective clothing to carry out the operation when removing the dyeing operation, and is comparatively troublesome. The formalin fumigation dyeing method has long disinfection time, toxic residues exist after disinfection, so the formalin fumigation dyeing method also needs residue neutralization, the whole process at least needs more than 24 hours, the time is long, and the formalin fumigation dyeing method is a common dyeing method with time-consuming and labor-consuming disinfection effects. In a preferred embodiment, the process of the present invention is selected from H2O2The generating device 7 is used as a decontamination mechanism, the biological safety cabinet comprises a working area and an airflow mixing area, the working area is provided with a socket, a hydrogen peroxide generator is electrically connected with the socket, the hydrogen peroxide generator comprises a hydrogen peroxide generating device and a hydrogen peroxide decomposition device, the airflow mixing area comprises an air suction fan and an air exhaust fan, and the air suction fan and the air exhaust fan are in an operating state under a decontamination state2O+O2The method has the advantages of no pollution and harm to the environment and personnel, high safety, no toxic residue, no need of neutralization, complete occurrence, dye removal, short time and high efficiency, and the decomposition process can be completed only in 3 hours.
The air suction fan and the exhaust fan are respectively connected with an air inlet filter and an exhaust filter, and in the embodiment, the air inlet filter and the exhaust filter are both high-efficiency air filters (HEPA).
In order to ensure the CO2Tightness between incubator and biosafety cabinet, as a preferred embodiment, the CO2Sealing gaskets 4 are arranged between the incubator and the biological safety cabinet and between the incubator and the flanges.
As an optional implementation mode, the sealing door is hinged to the input port of the biological safety cabinet through a hinge 6, in this state, the sealing door can be in various implementation modes such as manual, electric, pneumatic and hydraulic, and when the sealing door is in manual mode, the sealing door is close to CO2One side of the incubator is provided with a door handle from CO2The incubator is pushed in the single direction, and the other modes such as electric, pneumatic and hydraulic drive are also to open the sealing door to the side of the biological safety cabinet.
As another optional implementation mode, the left side and the right side of the input port of the biosafety cabinet are provided with slideways, the sealing door can be arranged in the slideways in a vertically sliding manner, the sealing door can be in various implementation forms such as manual, electric, pneumatic and hydraulic, and when the sealing door is in manual operation, the sealing door is close to the CO2One side of the incubator is provided with a door handle. Both of the above-mentioned sealing doors are provided on their outer side with a sealing structure, for example a sealing strip running around the sealing door panel.
In a specific application, the fertilized egg is in CO2When culturing in the incubator, the sealing door is closed, CO2The incubator is completely isolated from the biological safety cabinet, when fertilized eggs need to be transferred into the biological safety cabinet for treatment, the sealing door is opened, and the tray is manually lifted from the CO2The incubator is taken out to a biological safety cabinet. In other alternative embodiments, the CO2The inside of the incubator is provided with a mechanical arm, the working area of the biological safety cabinet is provided with a transverse slideway, the cultured cells/tissues are transferred to a tray of the slideway by the mechanical arm after the sealing door is lifted, and then the cells/tissues are manually slid to an operable space inside the safety cabinet.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (5)
1. Biological safety cabinet and CO2Integration embryonated egg modulation equipment of incubator, its characterized in that includes: CO 22Incubator and biosafety cabinet, said CO2CO is respectively arranged at the opposite sides of the incubator and the biological safety cabinet2Incubator outlet and biosafety cabinet inlet, CO2The output port of the incubator is connected with the input port of the biological safety cabinet through a flange, a decontamination mechanism is arranged in the biological safety cabinet, and the input port of the biological safety cabinet is provided with a sealing door capable of being opened and closed;
in a dye removing state, the dye removing mechanism operates, and the sealing door is opened; under the condition that the fertilized eggs are cultured after the degainting is finished, the degainting mechanism stops running, and the sealing door is closed; after the fertilized eggs are cultured, the sealing door is opened, and the cultured fertilized eggs are cultured from CO2The incubator was transferred to a biosafety cabinet.
2. The biosafety cabinet and CO according to claim 12The integrated fertilized egg modulating device of the incubator is characterized in that the dye removing mechanism comprises a hydrogen peroxide generator, the biological safety cabinet comprises a working area and an air flow mixing area, the working area is provided with a socket, the hydrogen peroxide generator is electrically connected with the socket, the hydrogen peroxide generator comprises a hydrogen peroxide generating device and a hydrogen peroxide decomposing device, the air flow mixing area comprises an air suction fan and an air exhaust fan, and the air suction fan and the air exhaust fan are in an operating state under the dye removing state.
3. The biosafety cabinet and CO according to claim 12The integrated fertilized egg preparation apparatus of the incubator, wherein the CO is2Sealing gaskets are arranged between the incubator and the biosafety cabinet and the flange。
4. The biosafety cabinet and CO according to claim 12The integrated fertilized egg preparing device of the incubator is characterized in that the sealing door is hinged to an input port of the biological safety cabinet.
5. The biosafety cabinet and CO according to claim 12The integrated fertilized egg preparing device of the incubator is characterized in that slide ways are arranged on the left side and the right side of the input port of the biological safety cabinet, and the sealing door is arranged in the slide ways in a vertically sliding manner.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010329241.6A CN111500446A (en) | 2020-04-23 | 2020-04-23 | Biological safety cabinet and CO2Integrated fertilized egg preparation equipment of incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010329241.6A CN111500446A (en) | 2020-04-23 | 2020-04-23 | Biological safety cabinet and CO2Integrated fertilized egg preparation equipment of incubator |
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| Publication Number | Publication Date |
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| CN111500446A true CN111500446A (en) | 2020-08-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202010329241.6A Pending CN111500446A (en) | 2020-04-23 | 2020-04-23 | Biological safety cabinet and CO2Integrated fertilized egg preparation equipment of incubator |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115404144A (en) * | 2022-09-30 | 2022-11-29 | 北京东联哈尔仪器制造有限公司 | Biological safety cabinet adopting magnetoelectric position switch |
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| CN103961728A (en) * | 2014-05-08 | 2014-08-06 | 苏州大学 | Transferring disinfecting device for pollutants in P3-grade biosafety cabinet |
| CN204865875U (en) * | 2015-07-31 | 2015-12-16 | 昆明倍健生物科技有限公司 | Multi -functional ultra -clean operation panel |
| CN105733929A (en) * | 2016-05-02 | 2016-07-06 | 刘瑞 | Biological incubator for genetic engineering |
| JP2017063709A (en) * | 2015-09-30 | 2017-04-06 | 憲隆 福永 | Culture chamber and culture facility |
| CN206290019U (en) * | 2016-12-26 | 2017-06-30 | 中南大学 | A kind of new cell culture chamber |
| CN206980782U (en) * | 2017-05-09 | 2018-02-09 | 黔南民族医学高等专科学校 | A kind of biomedical detection operating desk |
| CN108404172A (en) * | 2018-04-10 | 2018-08-17 | 军事科学院系统工程研究院卫勤保障技术研究所 | One plants case instrument integration vaporized hydrogen peroxide chlorination equipment |
| CN208166989U (en) * | 2018-03-30 | 2018-11-30 | 新昌县大成生物科技有限公司 | A kind of close Biohazard Safety Equipment |
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2020
- 2020-04-23 CN CN202010329241.6A patent/CN111500446A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103961728A (en) * | 2014-05-08 | 2014-08-06 | 苏州大学 | Transferring disinfecting device for pollutants in P3-grade biosafety cabinet |
| CN204865875U (en) * | 2015-07-31 | 2015-12-16 | 昆明倍健生物科技有限公司 | Multi -functional ultra -clean operation panel |
| JP2017063709A (en) * | 2015-09-30 | 2017-04-06 | 憲隆 福永 | Culture chamber and culture facility |
| CN105733929A (en) * | 2016-05-02 | 2016-07-06 | 刘瑞 | Biological incubator for genetic engineering |
| CN206290019U (en) * | 2016-12-26 | 2017-06-30 | 中南大学 | A kind of new cell culture chamber |
| CN206980782U (en) * | 2017-05-09 | 2018-02-09 | 黔南民族医学高等专科学校 | A kind of biomedical detection operating desk |
| CN208166989U (en) * | 2018-03-30 | 2018-11-30 | 新昌县大成生物科技有限公司 | A kind of close Biohazard Safety Equipment |
| CN108404172A (en) * | 2018-04-10 | 2018-08-17 | 军事科学院系统工程研究院卫勤保障技术研究所 | One plants case instrument integration vaporized hydrogen peroxide chlorination equipment |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115404144A (en) * | 2022-09-30 | 2022-11-29 | 北京东联哈尔仪器制造有限公司 | Biological safety cabinet adopting magnetoelectric position switch |
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