CN220294167U - Continuous preparation chromatographic device - Google Patents

Abstract

The utility model relates to the technical field of chromatography, in particular to a continuous preparation chromatographic device, which comprises a chromatographic column, wherein the top of the chromatographic column is communicated with a liquid outlet end of a three-way pipe, two liquid inlet ends of the three-way pipe are respectively communicated with a liquid outlet end of a four-way pressure supplementing assembly, and three liquid inlet ends of the four-way pressure supplementing assembly are respectively communicated with a liquid storage tank; the four-way pressure supplementing assembly comprises a four-way part, a liquid inlet end of the four-way part is communicated with the liquid storage tank, a liquid outlet end of the four-way part is communicated with a liquid inlet end of the three-way pipe, a pressurizing plate is vertically and slidably connected in the four-way part, the pressurizing plate is in transmission connection with a driving assembly, liquid leakage holes are formed in the pressurizing plate at equal intervals in the circumferential direction, and a non-return part is arranged on the liquid leakage holes. The utility model can realize hydraulic compensation, and the four-way pressure compensating assemblies at the two ends alternately compensate pressure, thereby realizing continuous injection of liquid in the chromatographic column.

Description

A continuous preparation chromatography device

Technical field

The utility model relates to the field of chromatography technology, in particular to a continuous preparation chromatography device.

Background technique

With the development of preparative liquid chromatography technology and the research and development of a large number of active substances, as well as the demand for purification and separation of new drugs, efficient, large-batch, green and energy-saving separation and purification technology has attracted more and more attention. The laboratory uses preparative liquid chromatography to separate and prepare pure substances on a small scale, which usually can only meet the needs of biological activity testing, toxicity studies and clinical trial research.

At present, conventional preparative chromatography requires advanced raw materials. After the raw materials are completed, the eluent is added, and then other materials are added. Manual control is required each time, which is laborious and cannot be produced continuously. The existing automated continuous preparative chromatography device passes Although the PLC-controlled four-way solenoid valve can realize continuous feeding, there will be a hydraulic loss after the liquid phase passes through the solenoid valve, resulting in low hydraulic pressure when entering the chromatographic column and poor separation effect of the chromatographic column. Therefore, a continuous preparative chromatography device is urgently needed. to solve.

Contents of the invention

The purpose of this utility model is to provide a continuous preparation chromatography device to solve the above problems.

In order to achieve the above purpose, the utility model provides the following solutions:

A continuous preparation chromatography device, including a chromatography column, the top of the chromatography column is connected to the liquid outlet end of a three-way tube, and the two liquid inlet ends of the three-way pipe are respectively connected to the liquid outlet end of a four-way pressure compensation component, so The three liquid inlet ends of the four-way pressure compensation component are respectively connected to liquid storage tanks;

The four-way pressure compensation assembly includes a four-way part, the liquid inlet end of the four-way part is connected to the liquid storage tank, the liquid outlet end of the four-way part is connected to the liquid inlet end of the three-way pipe, so There is a pressure plate connected vertically and slidingly in the four-way part, and the pressure plate is drivingly connected with a driving assembly. The pressure plate is provided with leakage holes at equal intervals in the circumferential direction, and the leakage holes are provided with backstops. department.

Preferably, the non-return part includes a one-way valve, and the one-way valve is disposed in the leakage hole.

Preferably, the driving assembly includes a threaded rod, the threaded rod is threadedly connected to the axis of the pressure plate, and a second driving part is drivingly connected to the top of the threaded rod.

Preferably, the second driving part includes a second motor, the fixed end of the second motor is fixed on the top of the four-way part, and the output shaft of the second motor is axially connected with the threaded rod.

Preferably, the four-way part includes an inner boosting pipe, the top side wall of the inner boosting pipe is provided with three channels at equal intervals in the circumference, and the bottom of the inner boosting pipe is connected to the liquid inlet end of the three-way pipe. The pressurizing plate is vertically slidably connected in the inner boosting pipe, and a connecting portion is coaxially sleeved outside the inner boosting pipe. The connecting portion communicates with the inner boosting pipe through the passage.

Preferably, the communication part includes a communication sleeve, the communication sleeve is coaxially sleeved outside the inner booster pipe, the communication sleeve is rotationally connected to the inner booster pipe, and the communication sleeve A first channel is provided on the top side wall. One end of the first channel is connected to any one of the channels, and the other end of the first channel is connected to any one of the liquid storage tanks. The connecting shaft sleeve is drivingly connected with First drive unit.

Preferably, the first driving part includes a first motor, a driving gear is connected to the output shaft of the first motor, and the driving gear is meshed with the bottom of the communicating sleeve.

Preferably, two vertical chute are symmetrically provided in the inner pressurizing pipe, and a clamping block is slidably connected in the chute, and the two clamping blocks are fixed at both ends of the pressurizing plate.

The utility model has the following technical effects: when in use, the tops of the three liquid storage tanks connected to the four-way pressure compensating assembly at both ends of the tee pipe are connected to the material, deionized water, and eluent respectively. Under the action of There is a hydraulic loss. At this time, the driving assembly drives the pressure plate to move up and down in the four-way part. When the pressure plate is pressed down, the liquid in the four-way part will be forced into the chromatographic column through the tee tube. At this time, the leakage hole will Under the action of the non-return part, the liquid is prevented from entering the upper part of the four-way part. When the pressure plate rises, the leakage hole opens, allowing the liquid to enter the lower part of the four-way part, thereby realizing hydraulic compensation. The four-way pressure compensation components at both ends Alternate backup pressure to achieve continuous injection of liquid into the chromatographic column.

Description of drawings

In order to explain the embodiments of the present invention or the technical solutions in the prior art more clearly, the drawings needed to be used in the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some of the drawings of the present invention. Embodiments, for those of ordinary skill in the art, other drawings can also be obtained based on these drawings without exerting creative labor:

Figure 1 is a schematic structural diagram of the utility model;

Figure 2 is a partial enlarged view of position A in Figure 1 of the present invention;

Figure 3 is a partial enlarged view of B in Figure 2 of the present invention;

Figure 4 is a top view of the four-way pressure compensation assembly of the present utility model;

Figure 5 is a schematic structural diagram of the pressure plate of the present utility model;

Among them, 1. Chromatographic column; 2. First motor; 3. Driving gear; 4. Connecting sleeve; 5. Internal booster tube; 6. First channel; 7. Connecting tube; 8. Liquid storage tank; 9. Tee; 10. Chute; 11. Threaded rod; 12. Pressure plate; 13. Channel; 14. Second motor; 15. Leakage hole; 16. One-way valve; 17. Clamp.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.

In order to make the above objects, features and advantages of the present invention more obvious and understandable, the present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments.

Referring to Figures 1 to 5, the present utility model provides a continuous preparation chromatography device, including a chromatography column 1. The top of the chromatography column 1 is connected to the liquid outlet end of a tee tube 9, and the two liquid inlet ends of the tee tube 9 are connected to each other. There is a liquid outlet end of the four-way pressure compensating assembly, and the three liquid inlet ends of the four-way pressure compensating assembly are respectively connected to a liquid storage tank 8;

The four-way pressure compensation assembly includes a four-way part, the liquid inlet end of the four-way part is connected with the liquid storage tank 8, the liquid outlet end of the four-way part is connected with the liquid inlet end of the tee pipe 9, and the four-way part is vertically slidably connected with a pressurized The pressure plate 12 and the pressure plate 12 are connected with a driving assembly. The pressure plate 12 is provided with leakage holes 15 at equal intervals in the circumferential direction, and the leakage holes 15 are provided with check portions.

When in use, the tops of the three liquid storage tanks 8 connected to the four-way pressure compensating components at both ends of the tee pipe 9 are respectively connected with the material, deionized water, and eluent. Under the action of the four-way pressure compensating components, the three-way pressure compensating components Both ends of the tube 9 enter the material or deionized water or eluent at the same time. Since injecting the chromatographic column 1 requires a certain injection pressure, there is a hydraulic loss after the material, deionized water, and eluent enter the four-way pressure compensation component. At this time, the driving assembly drives the pressure plate 12 to move up and down in the four-way part. When the pressure plate 12 is pressed down, the liquid in the four-way part will be forced into the chromatographic column 1 through the tee tube 9. At this time, the leakage hole 15 Under the action of the non-return part, the liquid is prevented from entering the upper part of the four-way part. When the pressure plate 12 rises, the leakage hole 15 is opened, which facilitates the liquid to enter the lower part of the four-way part, thereby realizing hydraulic compensation. The four-way valves at both ends The pressure compensating component alternates with the pressure compensating component to achieve continuous injection of liquid into the chromatographic column 1.

In a further optimized solution, the non-return part includes a one-way valve 16 , and the one-way valve 16 is arranged in the leakage hole 15 .

The one-way valve 16 is preferably a H41F-10S type one-way valve. A baffle can also be hinged at the bottom of the leakage hole 15. When the pressure plate 12 moves downward, the leakage hole 15 is closed under the action of the baffle. When the pressure plate 12 moves upward, the leakage hole 15 opens.

In a further optimized solution, the driving assembly includes a threaded rod 11, which is threadedly connected to the axis of the pressure plate 12, and a second driving part is drivingly connected to the top of the threaded rod 11.

In a further optimized solution, the second driving part includes a second motor 14 , the fixed end of the second motor 14 is fixed on the top of the four-way part, and the output shaft of the second motor 14 is axially connected to the threaded rod 11 .

The second motor 14 drives the threaded rod 11 to rotate, so that the pressure plate 12 can move up and down.

To further optimize the plan, the four-way part includes an inner booster pipe 5. Three channels 13 are opened at equal intervals in the circumferential direction on the top side wall of the inner booster pipe 5. The bottom of the inner booster pipe 5 is connected to the liquid inlet end of the tee pipe 9 to pressurize it. The plate 12 is vertically slidably connected to the inner booster pipe 5 . The outer coaxial sleeve of the inner booster pipe 5 is provided with a connecting portion. The connecting portion communicates with the inner booster pipe 5 through the channel 13 .

To further optimize the solution, the connecting part includes a connecting sleeve 4, which is coaxially located outside the inner boosting pipe 5. The connecting sleeve 4 is rotationally connected to the inner boosting pipe 5, and the top side wall of the connecting sleeve 4 is provided with The first channel 6 has one end connected with any channel 13, the other end of the first channel 6 connected with any liquid storage tank 8, and the connecting sleeve 4 is drivingly connected with a first driving part.

In a further optimized solution, the first driving part includes a first motor 2 , the output shaft of the first motor 2 is connected with a driving gear 3 , and the driving gear 3 is meshed with the bottom of the communicating sleeve 4 .

The first motor 2 drives the driving gear 3 to rotate. Under the meshing action of the driving gear 3 and the communicating sleeve 4, the driving gear 3 drives the communicating sleeve 4 to rotate, so that one end of the first channel 6 on the communicating sleeve 4 rotates. , is connected with the liquid outlet end of the communication pipe 7 at the bottom of the liquid storage tank 8, thereby allowing the liquid in one of the liquid storage tanks 8 to enter the first channel 6, and the other end of the first channel 6 is connected to the top of the inner booster pipe 5 The corresponding channels 13 opened on the side walls are connected, allowing the liquid to enter the inner booster tube 5 .

The liquid outlet end of the communication pipe 7 of the remaining liquid storage tank 8 is in contact with the outer wall of the communication sleeve 4 to prevent liquid leakage.

The first channel 6 can be designed as a reduced diameter structure, thereby increasing the liquid flow rate and increasing the injection pressure.

After the material injection is completed, the connecting sleeve 4 is driven by the driving gear 3 to rotate, so that the first channel 6 is connected with the liquid outlet end of the connecting pipe 7 of another liquid storage tank 8, thereby realizing the injection of other liquids.

The tee tube 9 is a metal tube. The tee tube 9 is fixedly connected and connected to the top of the chromatographic column 1 . The inner booster pipe 5 is fixedly installed at the end of the tee tube 9 .

To further optimize the solution, two vertically arranged chute 10 are symmetrically provided in the inner booster pipe 5 . The chute 10 is slidably connected with a clamping block 17 , and the two clamping blocks 17 are fixed at both ends of the pressure plate 12 .

There are two vertically arranged chute 10 symmetrically provided in the inner booster pipe 5. Two clamping blocks 17 are fixed on both ends of the pressure plate 12. The clamping blocks 17 and the chute 10 vertically slide and cooperate to prevent pressurization. The plate 12 rotates, and the pressure plate 12 moves up and down through the threaded rod 11 .

In the description of the present invention, it should be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", The orientations or positional relationships indicated by "horizontal", "top", "bottom", "inner", "outer", etc. are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the present invention, rather than indicating or It is implied that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation and is therefore not to be construed as a limitation of the invention.

The above-described embodiments only describe the preferred modes of the present invention and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, those of ordinary skill in the art can make various modifications to the technical solutions of the present invention. All deformations and improvements shall fall within the protection scope determined by the claims of the present invention.

Claims (8)

1. A continuous preparation chromatography device, characterized in that: it includes a chromatography column (1), the top of the chromatography column (1) is connected with the liquid outlet end of a tee tube (9), and the two ends of the tee tube (9) Each liquid inlet end is respectively connected to the liquid outlet end of the four-way pressure compensation assembly, and the three liquid inlet ends of the four-way pressure compensation assembly are respectively connected to a liquid storage tank (8); The four-way pressure compensation assembly includes a four-way part, the liquid inlet end of the four-way part is connected with the liquid storage tank (8), and the liquid outlet end of the four-way part is connected with the three-way pipe (9) The liquid inlet end is connected, and a pressurizing plate (12) is vertically and slidingly connected in the four-way part. The pressurizing plate (12) is transmission connected with a driving assembly. The pressurizing plate (12) is opened at equal intervals in the circumferential direction. There is a leakage hole (15), and the leakage hole (15) is provided with a non-return portion. 2. A continuous preparation chromatography device according to claim 1, characterized in that: the check part includes a one-way valve (16), and the one-way valve (16) is arranged in the leakage hole (15) Inside. 3. A continuous preparation chromatography device according to claim 2, characterized in that: the driving assembly includes a threaded rod (11), and the threaded rod (11) is threadedly connected to the axis of the pressure plate (12). , a second driving part is drivingly connected to the top of the threaded rod (11). 4. A continuous preparation chromatography device according to claim 3, characterized in that: the second driving part includes a second motor (14), and the fixed end of the second motor (14) is fixedly connected to the four-way At the top of the motor, the output shaft of the second motor (14) is axially connected with the threaded rod (11). 5. A continuous preparation chromatography device according to claim 1, characterized in that: the four-way part includes an internal booster tube (5), and the top side wall of the internal booster tube (5) is opened at equal intervals in the circumferential direction. There are three channels (13), the bottom of the inner booster pipe (5) is connected to the liquid inlet end of the tee pipe (9), and the pressure plate (12) is vertically slidingly connected to the inner booster pipe. (5), the inner booster tube (5) is coaxially provided with a communication portion on the outside, and the communication portion communicates with the inner booster tube (5) through the passage (13). 6. A continuous preparation chromatography device according to claim 5, characterized in that: the communication part includes a communication sleeve (4), and the communication sleeve (4) is coaxially sleeved in the inner booster tube. (5), the connecting sleeve (4) is rotationally connected to the inner booster pipe (5), and a first channel (6) is provided on the top side wall of the connecting sleeve (4). The first channel (6) is One end of the channel (6) is connected to any of the channels (13), the other end of the first channel (6) is connected to any of the liquid storage tanks (8), and the connecting shaft sleeve (4) drives The first driving part is connected. 7. A continuous preparation chromatography device according to claim 6, characterized in that: the first driving part includes a first motor (2), and a driving gear (3) is connected to the output shaft of the first motor (2). ), the driving gear (3) is meshed with the bottom of the communicating sleeve (4). 8. A continuous preparation chromatography device according to claim 5, characterized in that: two vertically arranged chute (10) are symmetrically provided in the inner booster tube (5), and the chute (10) There are clamping blocks (17) slidably connected inside, and the two clamping blocks (17) are fixed at both ends of the pressure plate (12).