CN220284109U - In-situ fixation sample preparation device for adherent cell transmission electron microscope sample - Google Patents
In-situ fixation sample preparation device for adherent cell transmission electron microscope sample Download PDFInfo
- Publication number
- CN220284109U CN220284109U CN202321413837.XU CN202321413837U CN220284109U CN 220284109 U CN220284109 U CN 220284109U CN 202321413837 U CN202321413837 U CN 202321413837U CN 220284109 U CN220284109 U CN 220284109U
- Authority
- CN
- China
- Prior art keywords
- connecting pipe
- frame body
- electron microscope
- transmission electron
- cell transmission
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000001464 adherent effect Effects 0.000 title claims abstract description 51
- 230000005540 biological transmission Effects 0.000 title claims abstract description 33
- 238000011065 in-situ storage Methods 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims description 6
- 238000005070 sampling Methods 0.000 claims description 13
- 239000004793 Polystyrene Substances 0.000 claims description 4
- 229920002223 polystyrene Polymers 0.000 claims description 4
- 238000000059 patterning Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 66
- 238000000034 method Methods 0.000 description 9
- 238000004519 manufacturing process Methods 0.000 description 4
- 210000002569 neuron Anatomy 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 108010019160 Pancreatin Proteins 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 238000010894 electron beam technology Methods 0.000 description 3
- 229940055695 pancreatin Drugs 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 238000004115 adherent culture Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000007790 scraping Methods 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 238000004627 transmission electron microscopy Methods 0.000 description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229910052762 osmium Inorganic materials 0.000 description 1
- SYQBFIAQOQZEGI-UHFFFAOYSA-N osmium atom Chemical compound [Os] SYQBFIAQOQZEGI-UHFFFAOYSA-N 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005464 sample preparation method Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
Landscapes
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides an in-situ fixed sample-customizing device for an adherent cell transmission electron microscope sample, which comprises a top cover, a hollow frame body and a detachable device bottom, wherein the top cover, the hollow frame body and the detachable device bottom are arranged from top to bottom: the frame body comprises a rectangular top surface and four side surfaces which are respectively connected with the top surface; the top cover is arranged on the frame body; the bottom end of the frame body is connected with the bottom of the device; the frame body also comprises a connecting pipe fixedly connected with the top surface; the connecting pipe is vertically arranged, and the upper end and the lower end of the connecting pipe are opened; the device bottom is provided with fixing rings protruding out of the upper surface of the device bottom, and the positions and the number of the fixing rings are respectively in one-to-one correspondence with the connecting pipes; the height of retainer plate is less than the height of connecting pipe, just the retainer plate snare is located on the lateral wall of connecting pipe, the adherence cell is cultivateed in the connecting pipe, adds the fixed back of fixed liquid, through the dismounting device end, separates fixed adherence cell from the connecting pipe, then carries out follow-up conventional section observation, reaches the cell and keeps original state, guarantees the effect of cell observation accuracy.
Description
Technical Field
The utility model relates to the field of scientific research equipment, in particular to a cell sample accommodating device, and more particularly relates to an in-situ fixation sample customizing device for an adherent cell transmission electron microscope sample.
Background
Order of (A)Before, when the transmission electron microscope is used for observing the adherent cells, most of the adherent cells cultured in vitro grow in a culture dish, soft silica gel hanging seeds or pancreatin with the concentration of 0.25% are needed to digest and centrifugally collect the cells, and then ultrathin sections are manufactured according to the conventional method, but the prior method not only can damage the cells, but also can change the original state of the cells, for example, the growth state of the nerve cells which need to observe specific neurons can not be realized.
In summary, the following problems exist in the prior art: the adherent cells are easy to be damaged, the accuracy of cell observation is affected, and certain special cells cannot be kept in the original state, so that the problem of electron microscope ultra-micro observation cannot be realized. Therefore, how to realize a device for fixing the adherent cells in situ to prepare samples, so that the adherent cells samples can be collected and fixed in the original state, the subsequent slicing specification and requirements can be directly met, and the step of the transmission electron microscope tabletting is simplified, so that the problems to be solved are solved.
Disclosure of Invention
The utility model provides an in-situ fixation sample-making device for an adherent cell transmission electron microscope sample, which is used for solving the problems.
An adherent cell transmission electron microscope sample in-situ fixation sampling device, comprising: top cap, hollow framework and detachable device bottom that top-down set up:
the frame body comprises a rectangular top surface and four side surfaces which are respectively connected with the top surface;
the top cover is arranged on the frame body;
the bottom end of the frame body is connected with the device bottom;
the frame body further comprises a connecting pipe fixedly connected with the top surface; the connecting pipe is vertically arranged, and the upper end and the lower end of the connecting pipe are open;
the device bottom is provided with fixing rings protruding out of the upper surface of the device bottom, and the positions and the number of the fixing rings are respectively in one-to-one correspondence with the connecting pipes; the height of the fixing ring is lower than that of the connecting pipe, and the fixing ring is sleeved on the outer side wall of the connecting pipe.
Preferably, the frame body includes a plurality of connection pipes, and the connection pipes are arranged at intervals.
Preferably, the connecting pipe is a cylindrical hole.
Preferably, the pipe diameter of the connecting pipe is 3mm-5mm, and the depth of the connecting pipe is 10mm-12mm.
Preferably, the thickness of the device bottom is 3mm-5mm.
Preferably, the length of the frame body is 5cm-7cm, the width of the frame body is 3cm-5cm, and the height of the frame body is 15mm-17mm.
Preferably, the height of the fixing ring is 1mm-3mm.
Preferably, the frame body is clamped with the device bottom.
Preferably, the top cover and the frame are made of polystyrene; the device bottom is made of soft rubber. The embodiment of the utility model has the beneficial effects that: through directly cultivateing the cell in this device connecting pipe, after the cell reaches suitable density, add the fixed liquid in the connecting pipe and fix the back, tear detachable device end down, fixed cell sample is taken out with the device end in the retainer plate to reach the normal position and fix, do not destroy the effect of the original state of cell, in addition, this device connecting pipe aperture size is suitable for follow-up electron microscope ultrathin section's specification, removes loaded down with trivial details work links such as repair piece from, lays good foundation for follow-up work.
Drawings
FIG. 1 is a schematic diagram of an in-situ immobilization sampling device for an adherent cell transmission electron microscope sample according to an embodiment of the present utility model;
FIG. 2 is a schematic view of the structure of the bottom of an in-situ immobilized sample-preparing device for an adherent cell transmission electron microscope sample according to an embodiment of the present utility model;
FIG. 3 is a schematic cross-sectional view of an in situ immobilization sampling device for an adherent cell transmission electron microscope sample according to an embodiment of the present utility model.
Reference numerals illustrate:
10. a device bottom; 101. a fixing ring; 20. a frame; 201. a connecting pipe; 30. and a top cover.
Detailed Description
The present utility model will now be described for a clearer understanding of technical features, objects, and effects of the present utility model.
As shown in fig. 1, an embodiment of the present utility model provides an in-situ immobilization sampling device for an adherent cell transmission electron microscope sample, including: top cap 30, hollow frame 20 and detachable device bottom 10 that top-down set up:
the frame 20 includes a rectangular top surface and four side surfaces connected to the top surface, respectively;
the top cover 30 is arranged on the frame body 20;
the bottom end of the frame body 20 is connected with the device bottom 10;
the frame 20 further includes a connection pipe 201 fixedly connected to the top surface; the connection pipe 201 is vertically arranged, and the upper and lower ends of the connection pipe 201 are opened;
as shown in fig. 2 and 3, the device bottom 10 is provided with fixing rings 101 protruding from the upper surface of the device bottom 10, and the positions and the number of the fixing rings 101 are respectively in one-to-one correspondence with the connecting pipes 201; the height of the fixing ring 101 is lower than that of the connecting pipe 201, and the fixing ring 101 is sleeved on the outer side wall of the connecting pipe 201.
The transmission electron microscope, short for transmission electron microscope, uses electron emitted by cathode, the electron beam formed by micropore in the center of anode penetrates through sample to obtain the electron signal of sample, and the electron signal is amplified to several hundred to several tens of thousands times by multi-stage electron such as objective lens, intermediate lens and projection lens, finally the image can be observed on fluorescent screen in real time, or projected on photographic film photo-sensitive film as permanent record transmission electron microscope to observe cell requirement. Since the transmission electron microscope penetrates the sample through the electron beam formed by the micro-hole in the center of the anode to obtain the electronic signal of the sample, the sample needs to be concentrated in a very small device for observation. Currently, conventional methods for cell transmission electron microscopy sampling: the method comprises the steps of growing in vitro cultured adherent cells in a culture dish, wherein after the adherent cells are cultured in the culture dish, the culture dish is usually a dish or a bottle with a larger bottom volume and cannot be directly used for transmission electron microscopy, so that the cultured adherent cells in the culture dish are scraped by a silica gel hanger or digested by pancreatin with the concentration of 0.25%, the scraped or digested adherent cells are centrifuged, the adherent cells are deposited at the bottom of a conical centrifuge tube, a fixing solution is added into the centrifuge tube for fixing, and then a fixed cell sample is taken out for preparing ultrathin slices according to a conventional method, but the method causes damage to the cells to different degrees.
According to the embodiment of the utility model, a penetrating connecting pipe 201 is arranged in a frame body 20, the bottom end of the connecting pipe 201 is connected with a device bottom plate, the connecting pipe 201 is preferably cylindrical due to the requirement of circular ultrathin section of a transmission electron microscope, adherent cells are placed in the connecting pipe 201 for culture, fixing liquid is added for fixing, and the bottom end of the connecting pipe 201 is connected with a device bottom 10 and corresponds to a fixing ring 101 on the device bottom 10 one by one, so that a cylindrical solid is formed after the adherent cells are fixed, the solid is distributed in the bottom end of the connecting pipe 201 and the fixing ring 101 of the device bottom 10, after the device bottom 10 is detached, a cylindrical cell fixing sample is taken out together with the device bottom, the cylindrical cell fixing sample is distributed in the fixing ring 101, and the adherent cells are directly fixed in situ in the connecting pipe 201, namely, the process of scraping the cells from a culture dish or adopting 0.25% pancreatin digestion and centrifugal collection is omitted, and the method of culturing and directly fixing is carried out in situ in the device. Because the aperture of the connecting pipe 101 in the embodiment of the utility model accords with the diameter specification of the electron microscope ultrathin section, a fixed cell sample can be sliced by a conventional ultrathin section method after being taken out from the fixing ring 101, and the transmission electron microscope observation is carried out, so that the sample preparation method for in-situ fixation of the adherent cells is realized.
The top cover 30 is disposed on the frame 20, and the top cover 30 may be directly covered on the frame 20, or may be connected to a corresponding side of the top cover 30 through one side of the frame 20, so that the top cover 30 is not separated from the frame 20 after being opened.
Preferably, the frame 20 includes a plurality of the connection pipes 201, and the plurality of connection pipes 201 are spaced apart. Setting up a plurality of connecting pipes 201 can realize carrying out the system appearance simultaneously to a plurality of experimental groups, carries out the sign near every connecting pipe 201 or the retainer plate 101 that corresponds, distinguishes different experimental groups, and the experiment of being convenient for is developed like this, and the interval setting also makes the fixed sample of different groups conveniently take out, can not produce mutual interference. Preferably, the connecting pipe 201 is a cylindrical hole. The connecting pipe 201 with the cylindrical hole is adopted, so that the device is simple to manufacture, saves materials and meets the specification requirement of the circular ultrathin section of the electron microscope.
Preferably, the pipe diameter of the connecting pipe 201 is 3mm-5mm, and the depth of the connecting pipe 201 is 10mm-12mm. The electron beam formed by the micropore in the center of the anode penetrates through the sample to obtain an electronic signal of the sample, the diameter of the sample is smaller, and when the sample is prepared by adopting the embodiment of the utility model, the sample needs to be directly sliced, so that the pipe diameter of the connecting pipe 201 for culturing the adherent cells is smaller, the pipe diameter of the connecting pipe 201 adopted by the embodiment of the utility model is 3mm-5mm, and the preferable use is 4mm, so that the fineness requirement of the transmission electron microscope on the sample is met. In the process of cell culture and fixation, the depth of the connecting pipe is 10mm-12mm, preferably 11mm, considering the added volumes of the culture medium and the fixing liquid, the adherent cells generally do not grow in an overlapping manner, the volume is very small, the adherent cells can be cultured and fixed in the connecting pipe without the inconvenient operation of the long connecting pipe, and the manufacturing cost of the connecting pipe 201 can be saved.
Preferably, the thickness of the device bottom 10 is 3mm-5mm. The device bottom 10 of the embodiment of the present utility model is connected to the frame 20 when the adherent cells are cultured, so that the adherent cells are in a space with a closed bottom, and when the adherent cells are cultured and fixed, the device bottom 10 needs to be disassembled, so that the fixed adherent cells can be taken out from the connecting pipe 201, and therefore, in order to facilitate the disassembly of the device bottom 10, the thickness of the device bottom 10 is set to be 3mm-5mm of soft rubber.
Preferably, the length of the frame body 20 is 5-7cm, the width of the frame body 20 is 3-5 cm, and the height of the frame body 20 is 15-17 mm. The frame body of the embodiment of the utility model is preferably a cuboid with the length of 6cm, the width of 4cm and the height of 16mm, the size can meet the requirements of 10mm-12mm of depth of the connecting pipe 10, and meanwhile, the size can meet the requirements of arranging a plurality of connecting pipes 201 according to the requirement, and the structure is stable, is not easy to fall down, occupies small space and is convenient to store.
Preferably, the height of the fixing ring 101 is 1mm-3mm.
When the adherent cells are cultured, the connecting pipe 201 and the device bottom 10 are connected together, but the connecting pipe 201 and the device bottom 10 are not fixedly connected, the connecting joint is closed, and a connecting joint is formed at the connecting joint, in order to increase the tightness of the connecting joint, a fixing ring 101 with a certain height is arranged on the device bottom 10 corresponding to the bottom end of the connecting pipe 201, the shape of the fixing ring 101 is consistent with that of the bottom end of the connecting pipe 201, and the fixing ring 101 is slightly larger than the pipe diameter of the connecting pipe, so that the bottom end of the connecting pipe 201 can be wrapped by the fixing ring 101, the leakage of the adherent cells in the culture and fixing processes is prevented, and meanwhile, the fixing ring 101 can play a fixing role after the device bottom 10 is detached, so that a solid cell fixing sample can be kept in place, and confusion can not occur when a plurality of groups of cells are simultaneously cultured. By combining the above functions of the fixing ring 101, the height of the fixing ring 101 is set to be 1mm-3mm, preferably 2mm, so that the functions can be realized, and the manufacturing cost can be saved.
Preferably, the frame 20 is engaged with the device bottom 10. The frame body 20 and the device bottom 10 can be connected and fixed through the fixing ring 101 on the device bottom 10, in order to enable the frame body 20 and the device bottom 10 to be connected more firmly, an upward protruding side edge is arranged on the device bottom 10, a recess corresponding to the side edge is arranged on the outer side of the frame body 20, after the protruding side edge on the device bottom 10 is contacted and clamped with the recess on the outer side of the frame body 20, the frame body 20 and the device bottom 10 are clamped, so that the frame body 20 and the device bottom 10 are connected more firmly, and the device bottom 10 is convenient to detach.
Preferably, the top cover and the frame body of the in-situ fixed sample preparation device for the adherent cell transmission electron microscope sample are manufactured by polystyrene; the device bottom is made of soft rubber. The embodiment of the utility model adopts the optical transparent polystyrene, is convenient for observing the adherent cells in culture, adopts the soft rubber at the bottom of the device, can increase the tightness of the connecting joint, is convenient for disassembly, and has low cost.
The embodiment of the utility model is used as one of the components in the preparation process of the adherent cell transmission electron microscope sample, plays a key role in providing a perfect and accurate adherent cell fixation sample, and ensures that the preparation of the transmission electron microscope sample can be efficiently, simply and conveniently completed. The brief process for preparing an adherent cell transmission electron microscope sample by adopting the embodiment of the utility model is as follows: the method comprises the steps of conducting adherent cell culture in a connecting pipe 201 of the device, removing culture solution by means of suction and the like after the adherent cell culture is finished, then washing with PBS for three times, adding glutaraldehyde with the concentration of 0.25%, fixing for three hours, washing with PBS for three times, washing with 1% osmium acid, fixing for 30 minutes, fixing the adherent cells on the bottom 10 of the device and the bottom end of the connecting pipe 201, removing the bottom 10 of the device, separating the fixed adherent cells from the connecting pipe, leaving the adherent cells in a fixing ring 101 of the bottom 10 of the device, taking a cell fixing sample exposed in the fixing ring 101 or stripping the cell fixing sample adhered to the bottom 10 of the device by adopting a needle, such as a needle, so as to take the cell fixing sample out of the device, washing the taken sample with PBS for three times, carrying out gradient dehydration on acetone, and observing the sample by a transmission electron microscope after replacement, infiltration, embedding, slicing and lead staining.
According to the embodiment of the utility model, the detachable device bottom 10 is adopted to separate the fixed adherent cells from the connecting pipe 201, and scraping or digestion is not needed, so that the cells are kept from being damaged outside experimental conditions, and the accuracy of cell observation is ensured. Meanwhile, the device can be used for fixing and sampling the electron microscope samples for the nerve cells needing to observe the growth condition of the nerve cells in situ, so that the original state of the cells is ensured.
The foregoing is illustrative of the present utility model and is not to be construed as limiting the scope of the utility model. In order that the components of the utility model may be combined without conflict, any person skilled in the art shall make equivalent changes and modifications without departing from the spirit and principles of the utility model.
Claims (9)
1. The in-situ fixation sample preparation device for the adherent cell transmission electron microscope sample is characterized by comprising a top cover (30), a hollow frame body (20) and a device bottom (10) which are arranged from top to bottom:
the frame body (20) comprises a rectangular top surface and four side surfaces which are respectively connected with the top surface;
the top cover (30) is arranged on the frame body (20);
the bottom end of the frame body (20) is detachably connected with the device bottom (10);
the frame body (20) further comprises a connecting pipe (201) fixedly connected with the top surface; the connecting pipe (201) is vertically arranged, and the upper end and the lower end of the connecting pipe (201) are open;
the device bottom (10) is provided with fixing rings (101) protruding out of the upper surface of the device bottom (10), and the positions and the number of the fixing rings (101) are respectively in one-to-one correspondence with the connecting pipes (201); the height of the fixing ring (101) is lower than that of the connecting pipe (201), and the fixing ring (101) is sleeved on the outer side wall of the connecting pipe (201).
2. The in situ fixation and sampling device for an adherent cell transmission electron microscope sample according to claim 1, wherein the frame (20) comprises a plurality of connecting pipes (201), and the plurality of connecting pipes (201) are arranged at intervals.
3. The in situ fixation and sampling device for an adherent cell transmission electron microscope sample according to claim 1, wherein the connecting tube (201) is a cylindrical hole.
4. The in-situ fixation sampling device for an adherent cell transmission electron microscope sample according to claim 2, wherein the pipe diameter of the connecting pipe (201) is 3mm-5mm, and the depth of the connecting pipe (201) is 10mm-12mm.
5. An adherent cell transmission electron microscope sample in situ fixation patterning device according to claim 1, characterised in that the thickness of the device base (10) is 3mm-5mm.
6. The in-situ immobilization sampling device for an adherent cell transmission electron microscope sample according to claim 1, wherein the length of the frame body (20) is 5cm-7cm, the width of the frame body (20) is 3cm-5cm, and the height of the frame body (20) is 15mm-17mm.
7. The in situ immobilization sampling device for an adherent cell transmission electron microscope sample according to claim 1, wherein the height of the fixing ring (101) is 1mm-3mm.
8. The in-situ fixation and sampling device for an adherent cell transmission electron microscope sample according to claim 1, wherein the frame body (20) is clamped with the device bottom (10).
9. The adherent cell transmission electron microscope sample in-situ fixation sampling device according to claim 1, wherein the top cover (30) and the frame (20) are made of polystyrene; the device bottom (10) sample culture is manufactured by soft rubber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321413837.XU CN220284109U (en) | 2023-06-05 | 2023-06-05 | In-situ fixation sample preparation device for adherent cell transmission electron microscope sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321413837.XU CN220284109U (en) | 2023-06-05 | 2023-06-05 | In-situ fixation sample preparation device for adherent cell transmission electron microscope sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220284109U true CN220284109U (en) | 2024-01-02 |
Family
ID=89338374
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321413837.XU Active CN220284109U (en) | 2023-06-05 | 2023-06-05 | In-situ fixation sample preparation device for adherent cell transmission electron microscope sample |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220284109U (en) |
-
2023
- 2023-06-05 CN CN202321413837.XU patent/CN220284109U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2007167002A (en) | Cassette for cell culture, tool for attaching and detaching cassette for cell culture and cell culture apparatus | |
| CN106544270B (en) | A kind of micro-fluidic chip and its cell culture processes co-cultured for cell | |
| JP5674953B2 (en) | Cell culture insert | |
| Sala et al. | Electrophysiological analysis of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) using multi-electrode arrays (MEAs) | |
| JPH053779A (en) | Cell culture insert | |
| CN102580794A (en) | Micro-fluidic chip capable of positioning cells and organisms and application thereof | |
| RU2014109864A (en) | SYSTEMS AND METHODS OF MULTILATERAL ANALYSIS | |
| CN220284109U (en) | In-situ fixation sample preparation device for adherent cell transmission electron microscope sample | |
| CN203411544U (en) | Aorta three-dimensional culture single cell separator | |
| JP2003180335A (en) | Storable culture vessel | |
| US6383813B1 (en) | Microfabrication of a nuclear transfer array for high-throughput animal cloning | |
| Stern et al. | The CellRaft AIRⓇ system: A novel system enabling organoid imaging, identification, and isolation | |
| CN110907252A (en) | Biological sample preparation device and sample preparation method thereof | |
| Chen et al. | Intestinal crypt organoid: isolation of intestinal stem cells, in vitro culture, and optical observation | |
| CN115612615A (en) | Culture dish convenient for observation for biological cell culture | |
| US20230032498A1 (en) | Frame and Method for Constructing Nerve Tract | |
| CN203429184U (en) | Multi-adherent-cell coculture device | |
| CN102367413B (en) | Quantitation concentrator of noctiluca scientillans living cells | |
| JP2004222545A (en) | Culture container with cell | |
| CN110296872A (en) | A kind of Respiratory Medicine sputum sampler | |
| CN217895641U (en) | Auxiliary device for obtaining complete organoid tissues | |
| US6767734B2 (en) | Method and apparatus for producing age-synchronized cells | |
| CN113325201B (en) | Method suitable for atomic force microscope observation of cell skeletons of perennial fruit trees | |
| CN217202788U (en) | Embryo culture dish | |
| CN101942499A (en) | Method for detecting cell apoptosis in germ cell-supporting cell coculture |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |