CN220149530U

CN220149530U - Nucleic acid extraction instrument and magnetic rod sleeve picking and placing mechanism

Info

Publication number: CN220149530U
Application number: CN202321057620.XU
Authority: CN
Inventors: 张建锦; 刘小明; 叶炳友
Original assignee: Shanghai 3D Medicines Co Ltd
Current assignee: Shanghai 3D Medicines Co Ltd
Priority date: 2023-05-05
Filing date: 2023-05-05
Publication date: 2023-12-08
Anticipated expiration: 2033-05-05

Abstract

The embodiment of the utility model provides a nucleic acid extraction instrument and a magnetic rod sleeve picking and placing mechanism, wherein the magnetic rod sleeve picking and placing mechanism comprises an upright post; the magnetic rod sleeve clamping moving part moves linearly along the first direction of the upright post, and the rotary clamping component of the magnetic rod sleeve clamping moving part is movably connected with the magnetic rod sleeve bracket and can rotate around the magnetic rod sleeve bracket; the magnetic rod sleeve clamping switching part comprises a driving source and a switching cam, the driving source is suitable for driving the switching cam to move linearly along the second direction of the upright post, so that the switching cam is connected with or disconnected from the rotary clamping assembly, and the first direction is perpendicular to the second direction. The magnetic rod sleeve picking and placing mechanism provided by the embodiment of the utility model is beneficial to improving the oscillation speed and frequency and improving the nucleic acid extraction effect and efficiency. On the other hand, the rotation of the rotary clamping assembly is driven by a switching cam which acts in the vertical direction, the structure is simple and reliable, the space layout is reasonable, the equipment space can be efficiently used, and the equipment volume is reduced.

Description

Nucleic acid extraction instrument and magnetic rod sleeve picking and placing mechanism

Technical Field

The embodiment of the utility model relates to the technical field of gene sequencing devices, in particular to a nucleic acid extractor and a magnetic rod sleeve picking and placing mechanism.

Background

In the field of nucleic acid extraction instruments, the extraction of nucleic acids by the magnetic bead method has been technically mature and applied on a large scale. Currently, magnetic bead nucleic acid extraction generally comprises four main steps of cleavage, binding, washing and elution. After the sample is cracked, adding a binding solution to enable the magnetic beads to be combined with the nucleic acid, inserting a magnetic rod sleeved with a magnetic rod sleeve into the cracking solution, adsorbing the magnetic beads on the surface of the magnetic rod sleeve under the action of a magnetic field, and transferring the magnetic beads into a washing solution by the magnetic rod to wash; after washing, the magnetic rod is pulled out from the magnetic rod sleeve, the magnetic rod sleeve loses magnetism, the magnetic beads are separated from the magnetic rod sleeve, the magnetic beads are transferred into the eluent, the nucleic acid attached to the magnetic beads is eluted, and then the eluent with the nucleic acid is transferred into the reaction tube for subsequent molecular diagnosis test.

In order to improve the degree of automation and the efficiency of nucleic acid extraction, the installation and the dismantlement of the magnetic rod sleeve in the kit are realized by adopting an automatic magnetic rod sleeve taking and placing mechanism gradually.

However, the current magnetic rod sleeve picking and placing mechanism still has the problem of poor nucleic acid extraction efficiency.

Disclosure of Invention

In view of the above, the embodiment of the utility model provides a nucleic acid extraction instrument and a magnetic rod sleeving and picking mechanism for improving the nucleic acid extraction effect and efficiency.

In order to achieve the above object, the embodiment of the present utility model provides the following technical solutions:

the embodiment of the utility model provides a magnetic rod sleeve picking and placing mechanism, which comprises the following components:

a column;

the magnetic rod sleeve clamping moving part is suitable for rectilinear movement along the first direction of the upright post, the magnetic rod sleeve clamping moving part comprises a magnetic rod sleeve support and a rotary clamping assembly, the rotary clamping assembly is movably connected with the magnetic rod sleeve support, and the rotary clamping assembly can rotate around the magnetic rod sleeve support;

the magnetic rod sleeve clamping and switching part comprises a driving source and a switching cam, wherein the driving source is arranged on the upright post, the driving source is suitable for driving the switching cam to linearly move along the second direction of the upright post, so that the switching cam is connected with or disconnected from the rotary clamping assembly, and the first direction is perpendicular to the second direction.

Optionally, the rotary clamping assembly includes:

the two ends of the clamping rotating shaft are respectively hinged to the magnetic rod sleeve bracket;

the clamping claw is arranged on the clamping rotating shaft;

and the cam matching part is fixed on the clamping rotating shaft and is suitable for being connected with or disconnected from the switching cam.

Optionally, the cam cooperation portion includes swing arm and leading wheel, the first terminal surface of swing arm is fixed in on the centre gripping rotation axis, the leading wheel set up in the second terminal surface of swing arm, the leading wheel can with the switching cam forms cam connection.

Optionally, the switching cam includes a first cam guiding surface and a second cam guiding surface, wherein a projection of a contour line of the first cam guiding surface forms an acute angle with the first direction, and the second cam guiding surface is connected with the first cam guiding surface and is located below the first cam guiding surface.

Optionally, the switching cam further includes a third cam guiding surface located below the second cam guiding surface, and an outer profile width of the switching cam of the third cam guiding surface area is smaller than an outer profile width of the switching cam of the second cam guiding surface area.

Optionally, the magnetic rod sleeve clamping switching part further comprises a guide shaft, a first end of the guide shaft is fixed to the switching cam, and a second end of the guide shaft is arranged on the upright post.

Optionally, the number of guide shafts is at least 2.

Optionally, the driving source comprises an electromagnet, a linear driving motor or a cylinder.

Optionally, the electromagnet includes a core connected to the switching cam and a return spring adapted to control the switching cam to be connected to or disconnected from the rotary clamping assembly.

Optionally, the magnetic rod sleeve picking and placing mechanism further comprises: the linear sliding rail module is arranged on the upright post and comprises a sliding block, a sliding block support and a linear guide rail, the sliding block is positioned between the sliding block support and the linear guide rail, the linear guide rail is fixed on the upright post, and the magnetic rod sleeve clamping moving part is fixed on the sliding block support.

Optionally, the magnetic rod sleeve picking and placing mechanism further comprises: the first end of the pressurizing elastic piece is fixed on the sliding block support, and the other end of the pressurizing elastic piece is connected with the rotary clamping assembly.

The embodiment of the utility model also provides a nucleic acid extraction instrument which comprises the magnetic rod sleeve picking and placing mechanism.

Compared with the prior art, the technical scheme of the embodiment of the utility model has the following advantages:

according to the magnetic rod sleeve picking and placing mechanism provided by the embodiment of the utility model, the driving source drives the switching cam to horizontally move to the position matched with the rotary clamping assembly, the rotary clamping assembly is utilized to rotate to achieve the grabbing of the magnetic rod sleeve, after the grabbing is finished, the switching cam of the magnetic rod sleeve clamping switching part is disconnected with the cam matching part of the magnetic rod sleeve clamping moving part, the rotary clamping assembly self-locks to clamp the magnetic rod sleeve, and the magnetic rod sleeve clamping moving part moves up and down in the whole vertical direction. It can be seen that the driving source and the magnetic rod sleeve clamping moving part which are rotated by the driving rotary clamping assembly can be separated, and the magnetic rod sleeve clamping moving part is light in weight and small in inertia when carrying the magnetic rod sleeve to oscillate, so that the oscillating speed and frequency are improved, and the nucleic acid extraction effect and efficiency are improved. On the other hand, the rotation of the rotary clamping assembly is driven by a switching cam which acts in the vertical direction, the structure is simple and reliable, the space layout is reasonable, the equipment space can be efficiently used, and the equipment volume is reduced.

Drawings

In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present utility model, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of a magnetic rod sleeve picking and placing mechanism;

fig. 2-3 are schematic structural diagrams of a magnetic rod sleeve picking and placing mechanism according to an embodiment of the present utility model;

fig. 4 is a schematic diagram of a partial structure of a magnetic rod sleeve picking and placing mechanism according to an embodiment of the present utility model;

FIG. 5 is a schematic diagram of another partial structure of a magnetic rod sleeve picking and placing mechanism according to an embodiment of the present utility model;

FIG. 6 is a schematic view of another partial structure of a magnetic rod sleeve picking and placing mechanism according to an embodiment of the present utility model;

FIG. 7 is a schematic view of another partial structure of a magnetic rod sleeve picking and placing mechanism according to an embodiment of the present utility model;

fig. 8 is a schematic diagram of a working process of the magnetic rod sleeve picking and placing mechanism provided by the embodiment of the utility model.

The device comprises a 1-mounting plate, a 10-magnetic rod sleeve plate, an 11-L-shaped connecting plate, a 12-rotating plate, a 13-return spring, a 14-rotating column, a 15-connecting plate, a 16-protecting frame, a 17-motor, a 18-screw rod and a 19-lifting plate, wherein the connecting plate is arranged on the mounting plate;

2-stand columns; 31-a magnetic rod sleeve bracket; 3-clamping the moving part by the magnetic rod sleeve; 32-a rotary clamping assembly; 301-clamping a rotating shaft; 302-claw; 300-cam mating portion; 3100-swing arm; 33-linear slide rail modules; 331-a slider bracket; 3200—guide wheels; 332-a slider; 333-linear guide rail; 4-clamping the switching part by the magnetic rod sleeve; 40-a driving source; 41-switching cams; 5-electromagnet; 50-a housing; 51-iron core; 52-a return spring; 6-a magnetic rod sleeve; 7-a pressing elastic member; 8-a guide shaft; 81-a first guide shaft; 82-a second guiding shaft.

Detailed Description

As known from the background art, the current magnetic rod sleeving and taking-out mechanism still has the problem of poor nucleic acid extraction efficiency.

The reasons for the poor nucleic acid extraction efficiency of the magnetic rod sleeve picking and placing mechanism are analyzed by combining the magnetic rod sleeve picking and placing mechanism.

Referring to fig. 1, fig. 1 is a magnetic rod sleeving and picking mechanism, which comprises a mounting plate 1, a rotation clamping assembly and a lifting assembly, wherein a protection frame 16 is installed at the bottom of the mounting plate 1, a connecting plate 15 is installed at the front end surfaces of the protection frame 16 and the mounting plate 1, a magnetic rod sleeving plate 10 is installed at the bottom of the mounting plate 1, the magnetic rod sleeving plate 10 is arranged at the rear side of the protection frame 16, the rotation clamping assembly is symmetrically arranged at the front side of the connecting plate 15, the rotation clamping assembly comprises a rotating column 14, a rotating plate 12 is installed on the rotating column 14, and a claw is fixed on the rotating column 14; the lifting assembly comprises a motor 17, the motor 17 is arranged at the bottom of the mounting plate 1, and the motor 17 drives the lifting plate 19 and the L-shaped connecting plate 11 to move up and down through a screw rod 18 so as to enable the rotating plate 12 to rotate. Each 2 clamping claws of the rotary clamping assembly are symmetrically arranged on the mounting plate, and under the action of the lifting assembly, the rotary plate 12 and the reset spring 13 which are fixed on the mounting plate, the clamping claws of the rotary assembly are driven to be automatically opened and closed, so that the function of automatically clamping the magnetic rod sleeve is realized.

However, the power source lifting component for realizing the rotation of the claw is directly arranged on the mounting plate, is integrated with the whole magnetic rod sleeve taking and placing mechanism, and has heavy whole weight. When the nucleic acid extraction works, the large-mass magnetic rod sleeve picking and placing mechanism has large motion inertia and low oscillation speed, so that the nucleic acid extraction efficiency is low.

In order to solve the above problems, the embodiment of the utility model provides a magnetic rod sleeve picking and placing mechanism, wherein a driving source drives a switching cam to horizontally move to a position matched with a rotary clamping assembly, the rotary clamping assembly is utilized to rotationally realize the grabbing of a magnetic rod sleeve, after the grabbing is finished, the switching cam of the magnetic rod sleeve clamping switching part is disconnected with a cam matching part of a magnetic rod sleeve clamping moving part, the rotary clamping assembly self-locks to clamp the magnetic rod sleeve, and the magnetic rod sleeve clamping moving part moves vertically up and down as a whole. It can be seen that the driving source and the magnetic rod sleeve clamping moving part which are rotated by the driving rotary clamping assembly can be separated, and the magnetic rod sleeve clamping moving part is light in weight and small in inertia when carrying the magnetic rod sleeve to oscillate, so that the oscillating speed and frequency are improved, and the nucleic acid extraction effect and efficiency are improved. On the other hand, the rotation of the rotary clamping assembly is driven by a switching cam which acts in the vertical direction, the structure is simple and reliable, the space layout is reasonable, the equipment space can be efficiently used, and the equipment volume is reduced.

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Referring to fig. 2-4, fig. 2-3 are schematic structural diagrams of a magnetic rod sleeving and picking mechanism according to different angles provided by an embodiment of the utility model; fig. 4 is a schematic structural view of a magnetic rod sleeve clamping movement part of a magnetic rod sleeve picking and placing mechanism according to an embodiment of the present utility model;

as shown in the figure, the magnetic rod sleeve picking and placing mechanism provided by the embodiment of the utility model comprises:

a column 2;

a bar magnet sleeve clamping moving part 3 adapted to linearly move along a first direction (Y direction in fig. 2) of the upright 2, the bar magnet sleeve clamping moving part 3 including a bar magnet sleeve bracket 31 and a rotary clamping assembly 32, the rotary clamping assembly 32 being movably connected to the bar magnet sleeve bracket 31, and the rotary clamping assembly 32 being rotatable about the bar magnet sleeve bracket 31;

the magnetic rod sleeve clamps the switching part 4 and comprises a driving source 40 and a switching cam 41, wherein the driving source 40 is arranged on the upright post 2, and the driving source 40 is suitable for driving the switching cam 41 to move linearly along a second direction (X direction in fig. 3) of the upright post 2, so that the switching cam 41 is connected with or disconnected from the rotary clamping assembly 32, and the first direction is perpendicular to the second direction.

The magnetic rod sleeve clamping moving part 3 is adapted to move linearly along a first direction of the upright 2, and when the upright 2 is regarded as a plate-like structure, the first direction refers to a length extending direction of the upright 2, and the first direction moves linearly, i.e. vertically up and down, in combination with fig. 2 and 3.

The rotary clamp assembly 32 is used to grasp or release the bar sleeve 6. The rotating clamping component 32 can rotate along the magnetic rod sleeve support 31, which means that when the magnetic rod sleeve needs to be grabbed, the rotating clamping component 32 can rotate around the magnetic rod sleeve support 31 to grab and clamp the magnetic rod sleeve 6 positioned below the magnetic rod sleeve support 31; when it is desired to release the bar magnet sleeve 6, the rotary clamp assembly 32 may be rotated about the bar magnet sleeve holder 31 to release the bar magnet sleeve 6.

Of course, in order to maintain the state that the rotating clamping assembly 32 grabs the magnetic rod sleeve, the magnetic rod sleeve picking and placing mechanism further comprises a pressurizing elastic member 7, a first end of the pressurizing elastic member 7 is fixed on the magnetic rod sleeve support 31 or other components fixedly connected with the magnetic rod sleeve support 31, and the other end of the pressurizing elastic member 7 is connected with the rotating clamping assembly 32. The pressing elastic member 7 may be a pressing spring, or may be an elastic component such as a torsion spring or a spring plate.

The driving source 40 is adapted to drive the switching cam 41 to move linearly in a second direction of the pillar 2, which refers to a thickness extending direction of the pillar 2 when the pillar 2 is seen as a plate-like structure, as shown in fig. 2 and 3, and the switching cam 41 is connected to or disconnected from the rotating clamping assembly 32 by moving linearly in the second direction to approach or separate from the rotating clamping assembly 32, that is, horizontally moving left and right.

The specific structure for realizing the linear motion of the magnetic rod sleeve clamping motion part 3 along the first direction of the upright post 2 is not limited. Specifically, in order to reduce the volume and to allow for simple structure and stable operation, the bar magnet sleeve picking and placing mechanism further includes a linear slide rail module 33, the linear slide rail module 33 is mounted on the upright post 2, and the linear slide rail module 33 is located between the bar magnet sleeve clamping movement portion 3 and the upright post 2. In one embodiment, the linear slide module 33 may include a slider 332, a linear guide 333, and a slider bracket 331 connecting the magnetic rod sleeve bracket 31 and the slider 332, wherein the linear guide 333 is fixed on the upright 2, the magnetic rod sleeve clamping moving part 3 is fixed on the slider bracket 331, and the slider 332 is disposed between the slider bracket 331 and the linear guide 333. The power mechanism drives the magnetic rod sleeve clamping moving part 3 and the sliding block 332 to move up and down along the linear guide rail 333, wherein the power mechanism can be a motor, a screw mechanism or a synchronous belt mechanism.

Of course, in other embodiments, in order to realize the linear motion of the magnetic rod sleeve clamping moving portion 3 along the first direction of the upright post 2, a linear groove may be formed on the upright post 2, the magnetic rod sleeve clamping moving portion 3 is provided with a protruding portion matched with the linear groove, and the protruding portion is embedded into the linear groove to realize the up-and-down motion of the magnetic rod sleeve clamping moving portion 3 along the linear groove.

With continued reference to fig. 3-4, in one particular embodiment, a self-rotating swivel clamp assembly 32 disposed on a bar magnet housing bracket 31 includes:

a clamping rotating shaft 301, wherein two ends of the clamping rotating shaft 301 are respectively hinged to the magnetic rod sleeve bracket 31; the grip rotation shaft 301 rotates around its axis;

a claw 302 disposed on the clamping rotation shaft 301, the claw 302 being adapted to grasp a magnetic rod sleeve;

and a cam engaging portion 300 fixed to the clamp rotary shaft 301 and adapted to be connected to or disconnected from the switching cam 41.

The rotational movement of the rotary clamping assembly 32 is accomplished by a horizontal linear movement of the drive source 40 through a cam mechanism connection. The cam matching portion 300 is configured to match the switching cam 41, and when the switching cam 41 moves horizontally and linearly in the second direction to below the cam matching portion 300, the cam matching portion 300 can be connected with the switching cam 41 by cam and rotate under the guidance of the switching cam 41 as the bar magnet sleeve clamping moving portion 3 continues to move downward.

The claws 302 are symmetrically disposed at two sides of the magnetic rod sleeve bracket 31, and the specific structure of the claws 302 is not limited as long as the grabbing effect on the magnetic rod sleeve can be achieved. In one embodiment, the number of claws 302 on each side of the bar magnet sleeve holder 31 is 2, and the claws are respectively fixed near two ends of the holding rotary shaft 301, and the positions of the claws are matched with the positions of the positioning holes of the bar magnet sleeve. The clamping claw 302 is embedded into the positioning hole to realize clamping and locking effects on the magnetic rod sleeve.

In a specific embodiment, to reduce sliding resistance and improve the motion stability of the mechanism, the cam matching portion 300 may include a swing arm 3100 and a guide wheel 3200, wherein a first end surface of the swing arm 3100 is fixed on the clamping rotation shaft 301, and the guide wheel 3200 is disposed on a second end surface of the swing arm 3100. The cam matching part 300 is matched with the switching cam 41 through the guide wheel 3200 to realize the rotation of the rotary clamping assembly 32 and grasp the magnetic rod sleeve, and the cam connection of the guide wheel 3200 and the switching cam 41 adopts high-pair rolling connection, so that the sliding resistance can be reduced, and the motion stability of the mechanism can be improved. When the bar magnet sleeve clamping moving part moves downwards along the first direction, a guide wheel 3200 on the swing arm 3100 contacts the switching cam 41 and acts along the surface of the switching cam 41, and the swing arm 3100 drives the claw 302 to rotate through the clamping rotating shaft 301.

Swing arm 3100 clamps rotation shaft 301, and claw 302 rotates around the axis of clamping rotation shaft 301 as a whole, thereby realizing clamping or loosening of claw 302 to the magnetic rod sleeve. As shown in fig. 4, one end of the pressing elastic member 7 is connected to the rotating clamping assembly 32, and the other end is connected to the slider bracket 331, so that the rotating clamping assembly 32 rotates in a direction of clamping the magnetic rod sleeve (the rotating direction is shown by the curved arrow in fig. 5). The pressurizing elastic piece 7 enables the claw 302 of the rotary clamping assembly 32 to rotate towards the direction of clamping the magnetic rod sleeve, so that the clamping of the magnetic rod sleeve is maintained, and the magnetic rod sleeve is self-locked. The rotary clamping assemblies 32 are symmetrically distributed in 1 set on the left and right. Under the action of the pressurizing elastic piece 7, the swing arm 3100 rotates inwards (in the direction close to the magnetic rod sleeve) to drive the clamping jaw 302 to clamp the magnetic rod sleeve and keep a self-locking state.

Of course, in other embodiments, the specific structure of the rotary clamping assembly 32 is not limited, as long as the structure of rotating around the bar sleeve holder 31 and grasping the bar sleeve can be realized. Similarly, in other embodiments, cam interface 300 may be in the form of swing arm 3100 directly, i.e., swing arm 3100 is cam coupled to switch cam 41.

The switching cam 41 moves horizontally in the second direction (direction X in fig. 3), i.e., in a direction perpendicular to the linear slide rail, and is not rotatable. Referring to fig. 6, in a specific embodiment, the switching cam 41 includes a first cam guiding surface a and a second cam guiding surface b. The projection of the contour line of the first cam guiding surface a forms an acute angle with the first direction (downward movement along the Y direction in fig. 3) so as to ensure that the magnetic rod sleeve clamping movement part 3 can rotate along the guiding surface of the switching cam in the downward movement process. The second cam guiding surface b is connected to and located below the first cam guiding surface a. The second cam guiding surface b may be a plane or a curved surface, and the extending direction of the second cam guiding surface b may be a vertical direction, and the extending direction of the second cam guiding surface b may be opposite to the extending direction of the first cam guiding surface. The first cam guiding surface a may be a flat surface or a curved surface.

In order to reduce running shock and improve stability of the mechanism, in a specific embodiment, the switching cam 41 further includes a third cam guiding surface, the third cam guiding surface is connected to and located below the second cam guiding surface b, and an outer contour width of the switching cam 41 in the third cam guiding surface area is smaller than an outer contour width of the switching cam 41 in the second cam guiding surface area. The outer contour width refers to the dimension in the Z direction in fig. 6.

Specifically, the switching cam 41 is formed by a plurality of guide surfaces, and the third cam guide surface may include guide surfaces c, d, which effectively reduce the impact during self-locking clamping. The switching cam 41 is cam-coupled with the guide wheel 3200 of the rotary clamping assembly 32, and the guide wheel 3200 moves along the guide surfaces a, b, c, d of the switching cam. The switching cam 41 is movable to a position in which it is cam-coupled with the guide wheel 3200 and also to a position in which it is separated from the guide wheel 3200 by the driving of the driving source 40. The switching cam 41 is normally in a state of being away from the guide wheel 3200. In other embodiments, the third cam guiding surface may also be a plane or a curved surface, the direction of extension of the third cam guiding surface being opposite to the direction of extension of the first cam guiding surface.

In order to reduce the moving wear and ensure the accuracy of the movement, a self-lubricating Polyoxymethylene (POM) material may be used for the switching cam 41. Of course, in other embodiments, the material of the switching cam is not limited as long as the friction coefficient requirement can be satisfied.

Referring to fig. 7, in order to guide the switching cam 41 to move horizontally along the second direction, the switching cam 41 may play a role in preventing the guiding wheel 3200 from rotating and stabilizing the guiding wheel 3200 when acting with the guiding wheel 3200, in a specific embodiment, the magnetic rod sleeve clamping switching portion 4 further includes a guiding shaft 8, a first end of the guiding shaft 8 is fixed to the switching cam 41, and a second end of the guiding shaft 8 is sleeved in the guiding hole of the upright 2.

The guide shaft 8 may be a special-shaped shaft, and the cross-sectional shape of the guide shaft 8 (the shape corresponding to the column guide hole) is not limited, and may be, for example, square or triangular as long as the function of preventing the switching cam 41 from rotating is achieved.

Of course, for ease of processing, the cross section of the guide shaft 8 may be circular, and in a specific embodiment, the number of the guide shafts 8 is at least 2, and the first guide shaft 81 and the second guide shaft 82 guide the switching cam 41 to move linearly, so as to prevent the switching cam 41 from rotating, and ensure reliable cam connection between the switching cam 41 and the swing arm 3100 of the clamping mechanism.

As shown in fig. 7, the magnetic rod sleeve clamping moving part 3 and the magnetic rod sleeve clamping power part are independent from each other, a gap is left between the guide wheel 3200 and the switching cam 41, and the magnetic rod sleeve clamping moving part 3 can oscillate up and down at high speed.

With continued reference to fig. 7, in one particular embodiment, the electromagnet 5 includes a housing 50, a core 51, and a return spring 52, the core 51 being disposed within the housing 50, the return spring 52 being adapted to control the switching cam 41 to contact or leave the guide wheel 3200. Of course, in other embodiments, the driving source 40 may be a linear driving motor or a cylinder, etc. capable of achieving linear motion.

The switching cam 41 of the magnetic rod sleeve holding switching portion 4 has a reset function realized by a reset spring 52 mounted on the electromagnet housing 50 and the iron core 51. The switching cam 41 normally disengages the swing arm 3100 of the rotating clamp assembly under the action of the return spring 52. After the electromagnet 5 is electrified at a high level, the iron core 51 overcomes the action of the reset spring 52, and the switching cam 41 integrally connected with the iron core 51 is driven to linearly move. In this way, when the swing arm 3100 of the clamping mechanism is required to operate, the switching cam 41 is required to be energized by the electromagnet 5, so that the energy consumption of the magnetic rod sleeve clamping switching part 4 can be reduced.

The structure of the magnetic rod sleeve picking and placing mechanism provided by the embodiment of the utility model is described above, and the working process of the magnetic rod sleeve picking and placing mechanism is correspondingly described below by taking a driving source as an electromagnet and combining with fig. 2-8.

1) Preparation for grabbing magnetic rod sleeve

The magnetic rod sleeve clamping moving part 3 moves from top to bottom to the upper part of the magnetic rod sleeve 6 along the linear guide 333 fixed on the upright post 2, the guide wheel 3200 is higher than the position near the first cam guide surface a, at this time, the switching cam 41 of the magnetic rod sleeve clamping switching part 4 is not contacted with the guide wheel 3200, the electromagnet 5 gives a high level, the switching cam 41 moves to the swing arm 3100 side along the second direction under the action of the electromagnet 5, and the state that the guide wheel 3200 and the switching cam 41 can form cam connection and the switching cam 41 cannot interfere with the swing arm 3100 is moved, and the switching cam 41 maintains the position state.

2) Grabbing and holding magnetic rod sleeve

The bar magnet sleeve clamping moving part 3 continues to move downwards, the guide wheel 3200 contacts with the first cam guide surface a of the switching cam 41, the bar magnet sleeve clamping moving part 3 continues to move downwards, the swing arm 3100 of the rotary clamping assembly 32 overcomes the torque of the pressurizing elastic piece 7 under the guide of the switching cam 41, the swing arm 3100 rotates outwards around the axis center of the clamping rotary shaft 301 (the rotating direction is shown by a curved arrow in fig. 8), the claw 302 of the rotary clamping assembly is opened, the guide wheel 3200 moves to the second cam guide surface b of the switching cam 41, the opening angle of the claw 302 is maximum, the head of the claw 302 exceeds the edge of the bar magnet sleeve, the bar magnet sleeve clamping moving part 3 continues to move downwards, the guide wheel 3200 continues to move along the second cam guide surface b of the switching cam 41, and the claw 302 maintains the maximum opening angle and moves to the lower side of the edge of the bar magnet sleeve. The bar magnet housing holding movement portion 3 continues to move downward, the guide wheel 3200 continues to move along the guide surfaces c, d of the switching cam 41, and the swing arm 3100 rotates inward about the axial center of the holding rotation shaft 301 (the rotation direction is shown by the curved arrow in fig. 5) and rotates in the direction of holding the bar magnet housing. At the same time, the torque applied by the pressing elastic member 7 decreases.

The electromagnet 5 is given a low level, and the switching cam 41 is separated from the guide wheel 3200 under the action of the return spring 52. After the guide wheel 3200 loses the support of the switching cam 41, the rotary clamping assembly 32 continues to rotate inward under the action of the pressing elastic member 7 until the claw 302 clamps the bar cover. The rotary clamping assembly 32 grabs and self-locks the clamping bar sleeve under the action of the pressurized elastic member.

3) High-speed high-frequency oscillation of magnetic rod sleeve clamping moving part 3

The magnetic rod sleeve clamping moving part 3 grabs and self-locks the magnetic rod sleeve to vibrate vertically along the linear guide rail 333 at high speed and high frequency, and the functions of cracking, eluting, extracting nucleic acid and the like of the biological sample are realized by combining the magnetic rod module.

4) Releasing magnetic bar sleeve

After the nucleic acid extraction is completed, the magnetic rod sleeve clamping movement part 3 moves along the vertical direction of the linear guide rail 333, moves to the position where the guide wheel 3200 is higher than the first cam guide surface a of the switching cam 41, at this time, the switching cam 41 of the magnetic rod sleeve clamping switching part 4 is not contacted with the guide wheel 3200, the electromagnet 5 is at a high level, the switching cam 41 moves to the swing arm 3100 side under the action of the iron core 51 of the electromagnet 5, and moves to the position where the guide wheel 3200 and the switching cam 41 can form cam connection and the switching cam 41 cannot interfere with the swing arm 3100, and the switching cam 41 maintains the position. The magnetic rod sleeve clamping moving part 3 moves downwards, the guide wheels 3200 of the rotary clamping assembly 32 are guided by the guide surfaces a and b of the switching cam 41, the rotary clamping assembly 32 rotates outwards, the clamping jaw 302 rotates towards the direction of releasing the magnetic rod sleeve until the head part of the clamping jaw 302 completely leaves the edge of the magnetic rod sleeve, and the magnetic rod sleeve breaks away from the rotary clamping assembly 32 under the dead weight, so that the function of releasing the magnetic rod sleeve is realized.

In order to solve the above problems, an embodiment of the present utility model further provides a nucleic acid extraction apparatus, which includes the above-mentioned magnetic rod sleeve picking and placing mechanism.

The driving source of the magnetic rod sleeve taking and placing mechanism drives the switching cam to horizontally move to a position matched with the rotary clamping assembly, the rotary clamping assembly is utilized to rotate to achieve grabbing of the magnetic rod sleeve, after grabbing is completed, the switching cam of the magnetic rod sleeve clamping and switching portion is disconnected with the cam matching portion of the magnetic rod sleeve clamping and moving portion, the rotary clamping assembly self-locks and clamps the magnetic rod sleeve, and the magnetic rod sleeve clamping and moving portion moves up and down in the whole vertical direction. It can be seen that the driving source and the magnetic rod sleeve clamping moving part which are rotated by the driving rotary clamping assembly can be separated, and the magnetic rod sleeve clamping moving part is light in weight and small in inertia when carrying the magnetic rod sleeve to oscillate, so that the oscillating speed and frequency are improved, and the nucleic acid extraction effect and efficiency are improved. On the other hand, the rotation of the rotary clamping assembly is driven by a switching cam which acts in the vertical direction, the structure is simple and reliable, the space layout is reasonable, the equipment space can be efficiently used, and the equipment volume is reduced.

Although the embodiments of the present utility model are disclosed above, the present utility model is not limited thereto. Various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the utility model, and the scope of the utility model should be assessed accordingly to that of the appended claims.

Claims

1. The utility model provides a mechanism is put to bar magnet cover, its characterized in that includes:

a column;

2. The bar magnet assembly of claim 1, wherein the rotating clamp assembly comprises:

the clamping claw is arranged on the clamping rotating shaft;

3. The bar magnet assembly and disassembly mechanism of claim 2, wherein the cam mating portion comprises a swing arm and a guide wheel, a first end surface of the swing arm is fixed on the clamping rotation shaft, the guide wheel is disposed on a second end surface of the swing arm, and the guide wheel can form cam connection with the switching cam.

4. The magnetic bar set pick-and-place mechanism of claim 1, wherein the switching cam includes a first cam guide surface and a second cam guide surface, a contour line projection of the first cam guide surface being at an acute angle to the first direction, the second cam guide surface being connected to and located below the first cam guide surface.

5. The bar magnet assembly and disassembly mechanism of claim 4, wherein the switching cam further comprises a third cam guide surface, the third cam guide surface being located below the second cam guide surface, and an outer profile width of the switching cam in the third cam guide surface area being smaller than an outer profile width of the switching cam in the second cam guide surface area.

6. The bar magnet assembly and disassembly mechanism of claim 1, wherein the bar magnet assembly and disassembly switching unit further comprises a guide shaft, a first end of the guide shaft is fixed to the switching cam, and a second end of the guide shaft is disposed on the upright.

7. The bar magnet assembly and disassembly mechanism of claim 6, wherein the number of guide shafts is at least 2.

8. The bar magnet assembly and disassembly mechanism of claim 1, wherein the drive source comprises an electromagnet, a linear drive motor, or a cylinder.

9. The bar magnet assembly of claim 8, wherein the electromagnet comprises a core and a return spring, the core being coupled to the switching cam, the return spring being adapted to control the switching cam to be coupled to or decoupled from the rotating clamp assembly.

10. The bar magnet assembly pick-and-place mechanism of any one of claims 1-9, further comprising: the linear sliding rail module is arranged on the upright post and comprises a sliding block, a sliding block support and a linear guide rail, the sliding block is positioned between the sliding block support and the linear guide rail, the linear guide rail is fixed on the upright post, and the magnetic rod sleeve clamping moving part is fixed on the sliding block support.

11. The bar magnet assembly and disassembly mechanism of claim 10, further comprising: the first end of the pressurizing elastic piece is fixed on the sliding block support, and the other end of the pressurizing elastic piece is connected with the rotary clamping assembly.

12. A nucleic acid extraction apparatus comprising the magnetic rod sleeve pick-and-place mechanism of any one of claims 1-11.