CN220132238U

CN220132238U - Cell culture liquid mixing arrangement with adjustable

Info

Publication number: CN220132238U
Application number: CN202321530528.0U
Authority: CN
Inventors: 苏雄杰
Original assignee: Shanghai Tiangong Biotechnology Co ltd
Current assignee: Shanghai Tiangong Biotechnology Co ltd
Priority date: 2023-06-15
Filing date: 2023-06-15
Publication date: 2023-12-05
Anticipated expiration: 2033-06-15

Abstract

The utility model relates to an adjustable cell culture fluid mixing device which comprises a tank body assembly, a mixing assembly and a temperature control assembly. The tank body assembly sequentially comprises a sealing cover plate, a tank body, a plurality of supporting legs and a control panel from top to bottom; the mixing component comprises a motor, and the output end of the motor is connected with a rotating rod; a plurality of blades are sleeved on the peripheral wall of the rotating rod at intervals; stirring rods are respectively arranged on the left side and the right side of the rotating rod; the driving gear coaxially fixed at the top end of the rotating rod is meshed with the driven gear coaxially fixed at the top end of the stirring rod; the temperature control assembly comprises a temperature sensor, a heating device, a refrigerating device and a control switch. The temperature of the liquid in the tank body is regulated and controlled by the temperature control component; the rotating rod drives a plurality of blades to mix the liquid; through the meshing between driving gear and the driven gear to make the stirring rod carry out reciprocating rotation, stir the liquid near heating device and refrigerating plant fast, be convenient for the temperature diffusion.

Description

Cell culture liquid mixing arrangement with adjustable

Technical Field

The utility model relates to the technical field of mixing devices, in particular to an adjustable cell culture solution mixing device.

Background

The culture fluid is an artificially prepared nutrient for the growth and maintenance of microorganisms, plants and animal tissues, generally contains carbohydrate, nitrogenous substances, inorganic salts, vitamins, water and the like, and is used for simulating in-vitro in-vivo environments so that cells can survive, grow and reproduce.

In order to prepare a culture medium suitable for the survival of cells in the outside, various substances required for the growth of various cells are mixed in the culture medium. Because the cell culture fluid has a very large number of components, the cell culture fluid must be thoroughly mixed prior to use to ensure that the nutrients in the fluid perform their intended functions. However, in actual operation, the shaking mixing is performed manually under the condition of much, the method not only consumes manpower and has low mixing efficiency, but also can not ensure the uniformity of force by the manual shaking, and can possibly have worse effect and destroy the culture solution; part of the cells need to be cultured in a special temperature environment, and the cell culture solution needs to be correspondingly temperature-regulated before being used so that the cells can be cultured at a proper temperature.

At present, an effective solution is not proposed for solving the problems of low mixing efficiency, poor mixing effect and incapability of adjusting the temperature of the cell culture solution existing in the related technology.

Disclosure of Invention

The utility model aims to overcome the defects in the prior art and provides an adjustable cell culture solution mixing device so as to solve the problems that the mixing efficiency of cell culture solution is low, the mixing effect is poor, the temperature of the cell culture solution cannot be adjusted and the like in the related art.

In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows:

an adjustable cell culture liquid mixing arrangement, includes jar body subassembly, set up in mixing element and the accuse temperature subassembly on the jar body subassembly, wherein:

the tank body assembly comprises a tank body, a sealing cover plate detachably arranged at the top end of the tank body and a plurality of supporting legs arranged at the bottom of the tank body, and a control panel is arranged on the outer wall of the front end of the tank body;

the mixing assembly comprises a motor arranged at the top end of the sealing cover plate, the output end of the motor is connected with a rotating rod, and the rotating rod penetrates through the sealing cover plate to be arranged and extends downwards to the bottom of the inner cavity of the tank body; a plurality of blades are sleeved on the peripheral wall of the rotating rod along the length direction of the rotating rod at intervals; stirring rods are respectively arranged on the left side and the right side of the rotating rod; the driving gear coaxially fixed at the top end of the rotating rod is meshed with the driven gear coaxially fixed at the top end of the stirring rod;

the temperature control assembly comprises a heating device and a refrigerating device which are respectively arranged at the left end and the right end of the middle part of the inner cavity of the tank body, wherein the temperature sensors are arranged at the bottom end of the inner cavity of the tank body; the temperature sensor, the heating device and the refrigerating device are respectively and electrically connected with the control panel.

Further, an output pipe is arranged on the side wall of the bottom end of the tank body, and a control valve is arranged on the output pipe; an input pipe is arranged on the side wall of the top end of the tank body.

Further, the bottom of the inner cavity of the tank body is obliquely arranged, and the lower end of the tank body is correspondingly communicated with the output pipe; the top end of the input pipe is detachably connected with a sealing cover.

Further, the driving gear sequentially comprises an inner gear ring, a connecting frame and an outer gear which are coaxially arranged from outside to inside; the connecting frame is arranged at the top of the driving gear and used for connecting the inner gear ring and the outer gear; the inner gear ring and the outer gear are of incomplete gear structures.

Further, the driven gear is arranged at the bottom of the connecting frame in a sliding manner and is positioned between the inner gear ring and the outer gear; the driven gears are respectively connected with the inner gear structure on the inner gear ring in an inner meshing manner and are connected with the outer gear structure on the outer gear in an outer meshing manner.

Further, the stirring rod passes through the sealing cover plate to be arranged and downwards extends to the inner cavity of the tank body, and a plurality of auxiliary rods are arranged at the upper end and the lower end of the stirring rod at intervals.

Further, a control switch, a temperature display and a control button are arranged on the control panel; the temperature display is electrically connected with the temperature sensor; the control button is electrically connected with the motor.

Further, the mixing assembly further comprises a mount, wherein:

the fixing frame is covered outside the motor, and the top end of the inner cavity of the fixing frame is fixed at the top end of the motor through a fixing bolt; the bottom edge of the fixing frame is fixed on the top end of the sealing cover plate through a fixing bolt.

Further, the driving gear is rotatably embedded in the middle upper part of the sealing cover plate; the top of the driving gear is flush with the top of the sealing cover plate and is in sliding connection with the bottom of the fixing frame.

Further, pulleys are arranged at the bottoms of the supporting legs.

Compared with the prior art, the utility model has the following technical effects:

(1) According to the adjustable cell culture solution mixing device, the temperature of the cell culture solution placed in the tank body is regulated and controlled through the heating device and the refrigerating device, so that cells are cultured at a proper temperature;

(2) According to the adjustable cell culture fluid mixing device, the plurality of blades are driven to rotate by the rotating rod, so that the cell culture fluid placed in the tank body is mixed;

(3) According to the adjustable cell culture liquid mixing device, the stirring rod is enabled to rotate in a reciprocating mode through the meshing of the driving gear and the driven gear, so that the cell culture liquid near the heating device and the refrigerating device is enabled to be stirred rapidly, and the temperature diffusion is facilitated.

Drawings

FIG. 1 is a schematic perspective view of an adjustable cell culture fluid mixing device according to the present utility model;

FIG. 2 is a schematic view showing a partial cross-sectional structure of an adjustable cell culture fluid mixing apparatus according to the present utility model;

FIG. 3 is a schematic view showing a partial cross-sectional structure of a tank assembly and a mixing assembly in an adjustable cell culture fluid mixing apparatus according to the present utility model;

FIG. 4 is a schematic partial cross-sectional perspective view of an adjustable cell culture fluid mixing device according to the present utility model;

FIG. 5 is a schematic perspective view of a driving gear and a driven gear in an adjustable cell culture fluid mixing device according to the present utility model;

FIG. 6 is a schematic partial perspective view of a mixing assembly of an adjustable cell culture fluid mixing device according to the present utility model.

Wherein the reference numerals are as follows:

10. a tank assembly; 11. a tank body; 12. sealing the cover plate; 13. support legs; 14. a control panel; 15. an output pipe; 16. a control valve; 17. an input tube; 18. sealing cover; 19. a pulley;

20. a mixing assembly; 21. a motor; 22. a rotating lever; 23. a blade; 24. a stirring rod; 25. a drive gear; 251. an inner gear ring; 252. a connecting frame; 253. an external gear; 26. a driven gear; 27. a fixing frame; 28. a control button;

30. a temperature control assembly; 31. a temperature sensor; 32. a heating device; 33. a refrigerating device; 34. a control switch; 35. a temperature display.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It should be noted that, without conflict, the embodiments of the present utility model and features of the embodiments may be combined with each other.

The utility model is further described below with reference to the drawings and specific examples, which are not intended to be limiting.

An adjustable cell culture fluid mixing device, as shown in fig. 1-4, comprises a tank assembly 10, a mixing assembly 20 and a temperature control assembly 30 arranged on the tank assembly 10.

The tank assembly 10 comprises a tank 11, a sealing cover plate 12 detachably arranged at the top end of the tank 11, and a plurality of supporting legs 13 arranged at the bottom of the tank 11, wherein a control panel 14 is arranged on the outer wall of the front end of the tank 11; the mixing assembly 20 comprises a motor 21 arranged at the top end of the sealing cover plate 12, the output end of the motor 21 is connected with a rotating rod 22, and the rotating rod 22 passes through the sealing cover plate 12 to be arranged and extends downwards to the bottom of the inner cavity of the tank 11; a plurality of blades 23 are sleeved on the peripheral wall of the rotating rod 22 along the length direction of the rotating rod at intervals; stirring bars 24 are respectively arranged on the left side and the right side of the rotating rod 22; the driving gear 25 coaxially fixed on the top end of the rotating rod 22 is meshed with the driven gear 26 coaxially fixed on the top end of the stirring rod 24; the temperature control assembly 30 comprises a temperature sensor 31 arranged at the bottom end of the inner cavity of the tank 11, a heating device 32 and a refrigerating device 33 respectively arranged at the left end and the right end of the middle part of the inner cavity of the tank 11; the temperature sensor 31, the heating device 32, and the cooling device 33 are electrically connected to the control panel 14, respectively.

Wherein, the temperature of the cell culture solution in the tank 11 is regulated and controlled by the heating device 32 and the refrigerating device 33 so as to culture the cells at a proper temperature; the rotating rod 22 drives the blades 23 to rotate so as to mix the cell culture fluid in the tank 11; the stirring rod 24 is made to reciprocate by the meshing of the driving gear 25 and the driven gear 26, so that the cell culture liquid near the heating device 32 and the refrigerating device 33 is rapidly stirred, and the temperature diffusion is facilitated.

In some of these embodiments, rotation of the stirring bar 24 is achieved by engagement between the driving gear 25 and the driven gear 26.

In some embodiments, the motor 21 drives the rotating rod 22 to rotate, and the rotating rod 22 drives the driving gear 25 to rotate, so that the two driven gears 26 meshed with the driving gear 25 rotate, and the stirring rod 24 rotates.

In some of these embodiments, the temperature sensor 31 detects the temperature of the cell culture fluid placed in the inner cavity of the tank 11 and transmits the temperature detection information to the control panel 14; the control panel 14 controls the heating device 32 and the refrigerating device 33 to be turned on and off according to a preset temperature, so as to heat or refrigerate the cell culture liquid; until the actual temperature of the cell culture liquid is the same as the preset temperature, the temperature sensor 31 transmits temperature detection information to the control panel 14; the control panel 14 controls the heating device 32 and the cooling device 33 to be turned off.

In some embodiments, the stirring bars 24 are 2, symmetrically arranged on the left and right sides of the rotating rod 22, and are respectively positioned near the heating device 32 and the refrigerating device 33.

In some embodiments, the heating device 32 and the cooling device 33 are external power sources, and the heating end of the heating device 32 and the cooling end of the cooling device 33 are both disposed in the inner cavity of the tank 11.

In some embodiments, the heating device 32 is a heater, and a heating tube thereof is disposed in the inner cavity of the tank 11; the refrigerating device 33 is a refrigerator, and a refrigerating sheet thereof is provided in the inner cavity of the tank 11.

In some embodiments, the number of the supporting legs 13 is 4, and the supporting legs are arranged at the bottom of the tank 11 at intervals.

Further, as shown in fig. 1-2, an output pipe 15 is arranged on the side wall of the bottom end of the tank 11, and a control valve 16 is arranged on the output pipe 15; an input pipe 17 is arranged on the side wall of the top end of the tank 11.

In some embodiments, the control valve 16 is a solenoid valve for controlling the opening and closing of the output tube 15, thereby controlling the discharge of the cell culture fluid.

Further, as shown in fig. 2, the bottom of the inner cavity of the tank 11 is obliquely arranged, and the lower end of the tank is correspondingly communicated with the output pipe 15; the top end of the input pipe 17 is detachably connected with a sealing cover 18.

Specifically, by obliquely setting the bottom of the inner cavity of the tank 11, it is avoided that liquid remains at the bottom of the inner cavity of the tank 11 when discharging the cell culture liquid.

In some of these embodiments, a sealing cap 18 is removably threaded onto the top end of the inlet tube 17 to prevent debris and dust from entering the interior cavity of the tank 11.

In some of these embodiments, the angle between the bottom of the inner cavity of the tank 11 and the horizontal direction is 1 ° to 5 °.

Further, the included angle between the bottom of the inner cavity of the tank 11 and the horizontal direction is 2-4 degrees.

Further, as shown in fig. 5 to 6, the driving gear 25 includes an inner gear ring 251, a connection carrier 252 and an outer gear 253 which are coaxially arranged in this order from the outside to the inside; the connection frame 252 is arranged on the top of the driving gear 25 and is used for connecting the inner gear ring 251 and the outer gear 253; the inner gear ring 251 and the outer gear 253 are of incomplete gear structure.

Further, the driven gear 26 is slidably disposed at the bottom of the connection frame 252 and is located between the inner gear ring 251 and the outer gear 253; the driven gears 26 are respectively connected with the gear structure of the inner gear ring 251 in an inner meshing manner and connected with the gear structure of the outer gear 253 in an outer meshing manner.

In some of these embodiments, positive rotation of driven gear 26 is achieved by the intermeshing connection of driven gear 26 with a gear structure on ring gear 251; the driven gear 26 is connected with the external gear 253 through external engagement with the gear structure so as to realize the reverse rotation of the driven gear 26. The driven gear 26 is connected with the inner gear ring 251 and the outer gear 253 in an alternate meshing manner, so that the driven gear 26 and thus the stirring rod 24 can reciprocally rotate.

Further, as shown in fig. 2 and 4, the stirring rod 24 is disposed through the sealing cover plate 12 and extends downward to the inner cavity of the tank 11, and a plurality of auxiliary rods are disposed at the upper and lower ends thereof at intervals.

Specifically, a plurality of auxiliary rods are arranged at the upper end and the lower end of the stirring rod 24 at intervals, and the auxiliary rods are not arranged at the middle part of the stirring rod, so that the heating device 32 or the refrigerating device 33 is avoided. The cell culture liquid near the upper end and the lower end of the heating device 32 and the refrigerating device 33 is rapidly stirred by a plurality of auxiliary rods, so that the temperature diffusion is facilitated.

In some of these embodiments, in order to smoothly rotate the stirring rod 24 and the rotating rod 22, bearings are sleeved on the top ends of the stirring rod 24 and the rotating rod 22. The bearing is arranged at the bottom end of the sealing cover plate 12 and positioned at the bottoms of the driving gear 25 and the driven gear 26; the outer rings of the 3 bearings are fixed on the sealing cover plate 12, and the inner rings of the 3 bearings are respectively sleeved on the outer peripheral walls of the corresponding stirring rod 24 and the corresponding rotating rod 22.

Further, as shown in fig. 1, the control panel 14 is provided with a control switch 34, a temperature display 35 and a control button 28; the temperature display 35 is electrically connected with the temperature sensor 31; the control button 28 is electrically connected to the motor 21.

Specifically, the control panel 14 is controlled to be turned on and off by the control switch 34; by arranging the temperature display 35, the temperature of the cell culture solution placed in the inner cavity of the tank 11 can be conveniently observed in real time; the agitation and stopping of the mixing assembly 20 is controlled by the control button 28.

Further, as shown in fig. 1-3, the mixing assembly 20 also includes a mount 27. Wherein, the fixing frame 27 is covered outside the motor 21, and the top end of the inner cavity is fixed on the top end of the motor 21 through a fixing bolt; the bottom edge of the fixing frame 27 is fixed to the top end of the sealing cover plate 12 by fixing bolts.

Further, as shown in fig. 2 to 3, the driving gear 25 is rotatably embedded in the middle upper portion of the sealing cover plate 12; the top of the driving gear 25 is flush with the top of the sealing cover plate 12 and is slidably connected to the bottom of the fixing frame 27.

Further, as shown in fig. 1 to 2, in order to facilitate movement of the can body assembly 10, the bottom of the support leg 13 is provided with a pulley 19.

Preferably, the pulley 19 is a brake-able pulley.

The application method of the utility model is as follows:

the cell culture liquid is poured into the inner cavity of the tank 11 from the input pipe 17, and the sealing cover 18 is detachably arranged on the input pipe 17.

The motor 21 is started by the control button 28, so that the motor 21 drives the rotating rod 22 to rotate, the rotating rod 22 drives the driving gear 25 to rotate, so that the two driven gears 26 meshed with the driving gear 25 rotate, and the stirring rod 24 rotates.

The control switch 34 is turned on, the temperature sensor 31 detects the temperature of the cell culture liquid placed in the inner cavity of the tank 11, and transmits temperature detection information to the control switch 34; the control switch 34 controls the heating device 32 and the refrigerating device 33 to be turned on and off according to a preset temperature, so as to heat or refrigerate the cell culture liquid; until the actual temperature of the cell culture fluid is the same as the preset temperature, the temperature sensor 31 transmits temperature detection information to the control switch 34; the control switch 34 controls the heating device 32 and the cooling device 33 to be turned off.

After the mixing is completed, the motor 21 and the control switch 34 are turned off; the mixed cell culture fluid is discharged through the outlet pipe 15.

The foregoing description is only illustrative of the preferred embodiments of the present utility model and is not to be construed as limiting the scope of the utility model, and it will be appreciated by those skilled in the art that equivalent substitutions and obvious variations may be made using the description and illustrations of the present utility model, and are intended to be included within the scope of the present utility model.

Claims

1. An adjustable cell culture fluid mixing arrangement, characterized in that includes jar body subassembly (10), set up in mixing arrangement (20) and accuse temperature subassembly (30) on jar body subassembly (10), wherein:

the tank body assembly (10) comprises a tank body (11), a sealing cover plate (12) detachably arranged at the top end of the tank body (11) and a plurality of supporting legs (13) arranged at the bottom of the tank body (11), and a control panel (14) is arranged on the outer wall of the front end of the tank body (11);

the mixing assembly (20) comprises a motor (21) arranged at the top end of the sealing cover plate (12), the output end of the motor (21) is connected with a rotating rod (22), and the rotating rod (22) penetrates through the sealing cover plate (12) to be arranged and extend downwards to the bottom of the inner cavity of the tank body (11); a plurality of blades (23) are sleeved on the peripheral wall of the rotating rod (22) along the length direction of the rotating rod at intervals; stirring rods (24) are respectively arranged on the left side and the right side of the rotating rod (22); a driving gear (25) coaxially fixed at the top end of the rotating rod (22) is meshed and connected with a driven gear (26) coaxially fixed at the top end of the stirring rod (24);

the temperature control assembly (30) comprises a temperature sensor (31) arranged at the bottom end of the inner cavity of the tank body (11), and a heating device (32) and a refrigerating device (33) which are respectively arranged at the left end and the right end of the middle part of the inner cavity of the tank body (11); the temperature sensor (31), the heating device (32) and the refrigerating device (33) are respectively and electrically connected with the control panel (14).

2. The adjustable cell culture fluid mixing device according to claim 1, wherein an output pipe (15) is arranged on the side wall of the bottom end of the tank body (11), and a control valve (16) is arranged on the output pipe (15); an input pipe (17) is arranged on the side wall of the top end of the tank body (11).

3. The adjustable cell culture fluid mixing device according to claim 2, wherein the bottom of the inner cavity of the tank body (11) is obliquely arranged, and the lower end of the tank body is correspondingly communicated with the output pipe (15); the top end of the input pipe (17) is detachably connected with a sealing cover (18).

4. The adjustable cell culture fluid mixing device according to claim 1, wherein the driving gear (25) comprises an inner gear ring (251), a connecting frame (252) and an outer gear (253) which are coaxially arranged in sequence from outside to inside; the connecting frame (252) is arranged at the top of the driving gear (25) and is used for connecting the inner gear ring (251) and the outer gear (253); the inner gear ring (251) and the outer gear (253) are of an incomplete gear structure.

5. The adjustable cell culture fluid mixing device according to claim 4, wherein the driven gear (26) is slidably arranged at the bottom of the connection frame (252) and is located between the inner gear ring (251) and the outer gear (253); the driven gears (26) are respectively connected with the inner gear structure of the inner gear ring (251) in an inner meshing way and are connected with the outer gear structure of the outer gear (253) in an outer meshing way.

6. The adjustable cell culture fluid mixing device according to claim 1, wherein the stirring rod (24) is arranged through the sealing cover plate (12) and extends downwards to the inner cavity of the tank body (11), and a plurality of auxiliary rods are arranged at the upper end and the lower end of the stirring rod at intervals.

7. The adjustable cell culture fluid mixing device according to claim 1, wherein a control switch (34), a temperature display (35) and a control button (28) are provided on the control panel (14); the temperature display (35) is electrically connected with the temperature sensor (31); the control button (28) is electrically connected with the motor (21).

8. The adjustable cell culture fluid mixing device according to claim 1, wherein the mixing assembly (20) further comprises a mount (27), wherein:

the fixing frame (27) is covered outside the motor (21), and the top end of the inner cavity of the fixing frame is fixed at the top end of the motor (21) through a fixing bolt; the bottom edge of the fixing frame (27) is fixed at the top end of the sealing cover plate (12) through a fixing bolt.

9. The adjustable cell culture fluid mixing device according to claim 8, wherein the driving gear (25) is rotatably embedded in the middle-upper part of the sealing cover plate (12); the top of the driving gear (25) is flush with the top of the sealing cover plate (12) and is in sliding connection with the bottom of the fixing frame (27).

10. An adjustable cell culture fluid mixing device according to claim 1, characterized in that the bottom of the support leg (13) is provided with a pulley (19).