CN220090560U - Expandable chromatographic separation and purification device - Google Patents

Abstract

An expandable chromatographic separation and purification device comprises a first liquid storage cavity, a first chromatographic skid, a first chromatographic column, a second liquid storage cavity, a second chromatographic skid, a second chromatographic column, a third liquid storage cavity, a third chromatographic skid, a third chromatographic column and a fourth liquid storage cavity; the first chromatographic sled, the second chromatographic sled and the third chromatographic sled at least comprise a driving pump and a pipeline which is detachably arranged, the driving pump drives a sample to be injected into the first chromatographic column, the second chromatographic column or the third chromatographic column through the pipeline, and the first chromatographic sled, the second chromatographic sled and the third chromatographic sled are respectively used for adapting to the chromatographic columns with the corresponding sizes through replacing the pipelines with different sizes; be provided with detachable pipeline in the chromatographic sled for after selecting the chromatographic column of required size to different samples, can be through changing the pipeline of corresponding size, make the chromatographic sled pour into the flow and the velocity of flow of required sample into for the chromatographic column, need not to change whole chromatographic sled, greatly reduced the quantity of purchasing the chromatographic sled, the cost is reduced.

Description

Expandable chromatographic separation and purification device

Technical Field

The utility model relates to the technical field of separation and purification of substances, in particular to a scalable chromatographic separation and purification device.

Background

Purification skids are used in manufacturing operations to provide fluid flow through column resins. The flow rate and velocity are critical to ensure proper separation of the fluid components. The unwanted components are separated and discarded, while the specified components are captured in pure form. The better the separation, the higher the quality of the given product.

The purification skid consists of pumps, flow meters and other instruments that work in concert to provide proper loading, separation and purification component capture for the chromatographic column. The flow rate required for a particular column depends on the size of the column, with larger columns, e.g. 2m columns, requiring a larger volume of fluid supply, while smaller columns, e.g. 0.8m columns, require significantly lower volumes.

In the existing chromatographic separation system, chromatographic columns with different sizes are required to be selected for different samples, and chromatographic sleds with corresponding sizes are purchased, so that a large number of chromatographic sleds are required to be purchased for adapting to different biological samples, the chromatographic sleds are used as modularized devices consisting of multiple instruments, the purchase cost is high, and huge cost pressure is brought to enterprises when a large number of chromatographic sleds are required to be purchased.

Disclosure of Invention

The utility model provides an expandable chromatographic separation and purification device which is used for solving the problem of high cost caused by the need of purchasing a large number of chromatographic pickups in order to meet the requirements of chromatographic separation and purification of different samples at present.

In one embodiment, an expandable chromatographic separation and purification device is provided, including a first liquid storage cavity, a first chromatographic sled, a first chromatographic column, a second liquid storage cavity, a second chromatographic sled, a second chromatographic column, a third liquid storage cavity, a third chromatographic sled, a third chromatographic column and a fourth liquid storage cavity which are sequentially communicated;

the first chromatographic sled is used for injecting a sample in the first liquid storage cavity into a first chromatographic column, and the first chromatographic column is used for carrying out first separation and purification on the sample and discharging the separated and purified sample into the second liquid storage cavity;

the second chromatographic sled is used for injecting the sample in the second liquid storage cavity into a second chromatographic column, and the second chromatographic column is used for carrying out second separation and purification on the sample and discharging the separated and purified sample into the third liquid storage cavity;

the third chromatographic sled is used for injecting the sample in the third liquid storage cavity into a third chromatographic column, and the third chromatographic column is used for separating and purifying the sample for the third time and discharging the separated and purified sample into the fourth liquid storage cavity;

the first chromatographic column, the second chromatographic column and the third chromatographic column are all detachably arranged in the chromatographic separation and purification device; the first chromatographic sled, the second chromatographic sled and the third chromatographic sled all include drive pump and demountable installation's pipeline at least, the drive pump passes through pipeline drive sample is poured into first chromatographic column, second chromatographic column or in the third chromatographic column, first chromatographic sled second chromatographic sled with third chromatographic sled is used for respectively through changing not equidimension the chromatographic column of pipeline adaptation response size.

In one embodiment, the conduit comprises dimensions of at least 2.0in, 1.5in, 1.0in, and 0.5in; and/or the dimensions of the first, second and third chromatography columns comprise at least 1.8m, 1.6m, 1.4m, 1.2m, 1.0m and 0.8m.

In one embodiment, the drive pump is provided with a plurality of drive gears having different drive flows and flow rates, respectively, each of the drive gears corresponding to a size of the conduit.

In one embodiment, one end of the tube is detachably connected to the drive pump, and the other end of the tube is detachably connected to the first chromatographic column, the second chromatographic column or the third chromatographic column.

In one embodiment, two ends of the pipeline are provided with connecting parts, and the pipeline is detachably installed through the connecting parts.

In one embodiment, one end of the pipe is detachably connected to the driving pump via a first connector, and the other end of the pipe is detachably connected to the first chromatographic column, the second chromatographic column or the third chromatographic column via a second connector.

In one embodiment, the first, second and third chromatography sleds each further comprise a flow valve and a flow meter disposed within the conduit.

In one embodiment, the drive pump is removably mounted within the first chromatographic sled, the second chromatographic sled, or the third chromatographic sled.

In one embodiment, the drive pump is fixedly connected to the conduit, and the drive pump and the conduit are adapted to be removed and installed together.

In one embodiment, a scalable chromatographic separation purification device is provided, comprising a chromatographic column and a chromatographic sled;

the chromatographic sled is used for injecting a sample into a chromatographic column, and the chromatographic column is used for separating and purifying the sample;

the chromatographic column is detachably arranged in the chromatographic separation and purification device; the chromatographic sled at least comprises a driving pump and a pipeline which is detachably arranged, wherein the driving pump drives a sample to be injected into the chromatographic column through the pipeline, and the chromatographic sled is respectively used for adapting to the chromatographic column with the corresponding size through replacing the pipeline with different sizes.

According to the expandable chromatographic separation and purification device, as the detachable pipeline is arranged in the chromatographic sled, after the chromatographic column with the required size is selected for different samples, the chromatographic sled can be matched with the chromatographic column by replacing the pipeline with the corresponding size, so that the flow and the flow velocity of the required sample are injected into the chromatographic column by the chromatographic sled, the whole chromatographic sled is not required to be replaced, the number of purchased chromatographic sleds is greatly reduced, and the cost is reduced; and the replaceable pipeline is convenient for maintenance of the chromatographic pry.

Drawings

FIG. 1 is a schematic structural diagram of a scalable chromatographic separation and purification device in one embodiment;

FIG. 2 is a schematic diagram of the connection of a chromatographic sled and a chromatographic column according to one embodiment;

wherein the reference numerals are as follows:

11-a first liquid storage cavity, 12-a second liquid storage cavity, 13-a third liquid storage cavity and 14-a fourth liquid storage cavity;

21-first chromatographic skid, 22-second chromatographic skid, 23-third chromatographic skid; 211-drive pump, 212-conduit, 213-first connection, 214-second connection;

31-first chromatography column, 32-second chromatography column, 33-third chromatography column.

Detailed Description

For pharmaceutical manufacturing, companies require equipment of specific dimensions to meet the process flow of the entire purification process. Manufacturing in the purification process requires a chromatographic column and a chromatographic skid of specific dimensions to meet the required flow and flow rate. These dimensions depend on the quantity and concentration of the product to be treated. The current column size for high volume pharmaceutical production ranges from 0.4m column (0.4 m refers to the diameter of the resin bed) to 2.0m column.

For the purification operation, the size of the chromatographic skid needs to be adjusted according to the size of the chromatographic column. The size of the chromatographic skid inner conduit or slide ensures that the proper flow rate is achieved on a chromatographic column of the specified size. The size of the chromatographic skid depends on the size of the chromatographic column used.

For multi-product manufacturing, this requires a significant capital investment to purchase the necessary skid of different sized columns. This is especially true for CDMO companies that own various future products unknown.

Typically, the size of the chromatographic skid ranges from 0.5in (feet in units) to 2.0in, which refers to the inner diameter of the tubing within the chromatographic skid. Businesses need to use many different sized columns for a given process, especially for multi-product facilities.

Examples of chromatographic column and sled chromatographic combinations in a typical chromatographic separation and purification manufacturing facility are provided below:

the manufacturing facility uses a standard 3-column purification process:

product A

The first purification step requires: 1.8m chromatographic column (2.0 in chromatographic skid)

The second purification step requires: 1.0m chromatographic column (1.5 in chromatographic skid)

The third purification step requires: 0.8m chromatographic column (1.0 in chromatographic skid)

Product B

The first purification step requires: 1.0m chromatographic column (1.5 in chromatographic skid)

The second purification step requires: 1.0m chromatographic column (1.5 in chromatographic skid)

The third purification step requires: 1.0m chromatographic column (1.0 in chromatographic skid)

Product C

The first purification step requires: 1.8m chromatographic column (2.0 in chromatographic skid)

The second purification step requires: 1.4m chromatographic column (2.0 in chromatographic skid)

The third purification step requires: 1.4m chromatographic column (2.0 in chromatographic skid)

Product D

The first purification step requires: 1.0m chromatographic column (1.0 in chromatographic skid)

The second purification step requires: 1.0m chromatographic column (1.0 in chromatographic skid)

The third purification step requires: 1.0m chromatographic column (1.0 in chromatographic skid)

Wherein, the diameter of the resin bed of the chromatographic column is 1.8m, and the diameter of the pipeline in the chromatographic sledge is 1.5in.

Current chromatographic sled designs, either custom built indoor (stick) or mobile sled, require a large number of chromatographic sleds to meet any of these combinations. For CDMO (under the three-step process), 3 sets of 2.0in, 3 sets of 1.5in and 3 sets of 1.0in chromatographic prys are required to be purchased, and 9 sets of chromatographic prys are required to be purchased, so that a large number of different types of chromatographic prys are required to be matched with different chromatographic columns, the cost is high, and the maintenance of the chromatographic prys is inconvenient.

The reason for the need to purchase a large number of chromatography sleds is that the current chromatography sleds are a non-detachable modular device, and a large number of chromatography sleds must be purchased for adapting to different chromatography columns, which results in high purchase cost for enterprises.

Based on the analysis, the utility model designs the detachable and expandable chromatographic sled, the pipelines in the chromatographic sled are detachably installed, when a chromatographic column is selected for a specific sample, the whole chromatographic sled is not required to be replaced, only the pipelines in the chromatographic sled are required to be replaced, other instruments and parts in the chromatographic sled are not required to be replaced, and the cost is greatly reduced as a universal part. For the standard 3-column purification process, three chromatographic prys are purchased.

Compared with the product A, the product B, the product C and the product D, the existing mode needs to purchase three sets of 2.0-in chromatographic sleds, two sets of 1.5-in chromatographic sleds and three sets of 1.0-in chromatographic sleds which are 8 sets, and more sets of chromatographic sleds are needed if more products are needed to be purified. In the utility model, three sets of expandable chromatographic pryes are adopted to realize the separation and purification of any product. Therefore, the cost can be greatly reduced by adopting the expandable chromatographic sled.

The expandable chromatographic sled can be used for replacing pipelines with different sizes, and can be realized by driving parts such as a pump or a flow valve in order to realize driving of different flow rates and flow velocities.

In order to meet the use requirements of more scenes, the driving pump in the expandable chromatographic sled can be detachably arranged to meet the requirements of different flow driving.

In the utility model, the detachable pipeline and/or the driving pump are arranged in the expandable chromatographic sled, which is also beneficial to the maintenance of the pipeline and the driving pump, for example, when the pipeline or the driving pump corrodes or leaks after long-term use, the pipeline or the driving pump can be detached and replaced with a new pipeline or driving pump without replacing the whole chromatographic sled.

The utility model will be described in further detail below with reference to the drawings by means of specific embodiments. Wherein like elements in different embodiments are numbered alike in association. In the following embodiments, numerous specific details are set forth in order to provide a better understanding of the present utility model. However, one skilled in the art will readily recognize that some of the features may be omitted, or replaced by other elements, materials, or methods in different situations. In some instances, related operations of the present utility model have not been shown or described in the specification in order to avoid obscuring the core portions of the present utility model, and may be unnecessary to persons skilled in the art from a detailed description of the related operations, which may be presented in the description and general knowledge of one skilled in the art.

Furthermore, the described features, operations, or characteristics of the description may be combined in any suitable manner in various embodiments. Also, various steps or acts in the method descriptions may be interchanged or modified in a manner apparent to those of ordinary skill in the art. Thus, the various orders in the description and drawings are for clarity of description of only certain embodiments, and are not meant to be required orders unless otherwise indicated.

The numbering of the components itself, e.g. "first", "second", etc., is used herein merely to distinguish between the described objects and does not have any sequential or technical meaning. The term "coupled" as used herein includes both direct and indirect coupling (coupling), unless otherwise indicated.

In one embodiment, an expandable chromatographic separation and purification device is provided, and three expandable chromatographic pryes are arranged in the chromatographic separation and purification device, so that the flow and flow velocity driving requirements of separation and purification of most biological samples can be met. The three sets of expandable chromatographic pickups are used as universal chromatographic pickups, and different pipelines can be replaced according to chromatographic columns with different sizes, so that the requirements of corresponding flow and flow velocity driving are met.

The detachable pipeline is arranged in the chromatographic sled of the chromatographic separation and purification device, so that after chromatographic columns with required sizes are selected for different samples, the chromatographic sled and the chromatographic columns can be matched by replacing the pipeline with the corresponding sizes, so that the flow and the flow velocity of the required samples are injected into the chromatographic columns by the chromatographic sled without replacing the whole chromatographic sled, the number of purchasing the chromatographic sled is greatly reduced, and the cost is reduced; and the replaceable pipeline is convenient for maintenance of the chromatographic pry.

Referring to fig. 1 and 2, the chromatographic separation and purification device of the present embodiment mainly includes a first liquid storage chamber 11, a first chromatographic sled 21, a first chromatographic column 31, a second liquid storage chamber 12, a second chromatographic sled 22, a second chromatographic column 32, a third liquid storage chamber 13, a third chromatographic sled 23, a third chromatographic column 33 and a fourth liquid storage chamber 14. The first liquid storage cavity 11, the first chromatographic skid 21, the first chromatographic column 31, the second liquid storage cavity 12, the second chromatographic skid 22, the second chromatographic column 32, the third liquid storage cavity 13, the third chromatographic skid 23, the third chromatographic column 33 and the fourth liquid storage cavity 14 are sequentially and directly or indirectly communicated, for example, the first liquid storage cavity, the first chromatographic skid 21, the first chromatographic column 31, the second liquid storage cavity, the second chromatographic skid 22, the second chromatographic column 32, the third liquid storage cavity 13, the third chromatographic skid 23, the third chromatographic column 33 and the fourth liquid storage cavity 14 can be directly communicated in a butt joint mode, or the indirect communication can be realized by arranging transition connectors such as connecting pipes at the joints.

The first liquid storage cavity 11, the second liquid storage cavity 12, the third liquid storage cavity 13 and the fourth liquid storage cavity 14 can be a liquid storage tank or a liquid storage tank and the like with a liquid storage cavity structure. The first liquid storage cavity 11, the second liquid storage cavity 12, the third liquid storage cavity 13 and the fourth liquid storage cavity 14 are four mutually independent cavities, and the four liquid storage cavities are respectively used for storing sample solutions in different purification stages. The first liquid storage cavity 11, the second liquid storage cavity 12, the third liquid storage cavity 13 and the fourth liquid storage cavity 14 are respectively provided with a liquid inlet and a liquid outlet, the liquid inlet is used for injecting sample solution, and the liquid outlet is used for discharging sample solution.

The first chromatographic sled 21, the second chromatographic sled 22 and the third chromatographic sled 23 are expandable chromatographic sled, and the first chromatographic sled 21, the second chromatographic sled 22 and the third chromatographic sled 23 can have the same structure, and the first chromatographic sled 21, the second chromatographic sled 22 and the third chromatographic sled 23 are all used for providing the driving of specific flow and flow rate for the sample solution.

The first chromatographic column 31, the second chromatographic column 32 and the third chromatographic column 33 may also be referred to as chromatographic columns, and the first chromatographic column 31, the second chromatographic column 32 and the third chromatographic column 33 are respectively used for performing chromatographic separation and purification on the sample solution so as to realize the tertiary chromatographic separation and purification on the sample solution and obtain the required sample substance. The first, second and third chromatographic columns 31, 32 and 33 may be cation or ion exchange chromatography.

In the chromatographic separation and purification device, a first chromatographic sled 21 is used for injecting a sample solution in a first liquid storage cavity 11 into a first chromatographic column 31, the first chromatographic column 31 is used for carrying out first separation and purification on the sample solution, and the separated and purified sample solution is discharged into a second liquid storage cavity 12;

the second chromatographic skid 22 is used for injecting the sample solution in the second liquid storage cavity 12 into the second chromatographic column 32, and the second chromatographic column 32 is used for separating and purifying the sample solution for the second time and discharging the separated and purified sample solution into the third liquid storage cavity 13;

the third chromatographic sled 23 is used for injecting the sample solution in the third liquid storage cavity into the third chromatographic column 33, the third chromatographic column 33 is used for separating and purifying the sample solution for the third time, and discharging the separated and purified sample solution into the fourth liquid storage cavity 14; and (5) finishing three chromatographic separation and purification.

In this embodiment, the first chromatographic column 31, the second chromatographic column 32 and the third chromatographic column 33 are detachably installed in the chromatographic separation and purification device, respectively, and the first chromatographic column 31, the second chromatographic column 32 and the third chromatographic column 33 can select chromatographic columns with corresponding sizes according to different purification steps of the purified product. The liquid inlet and the liquid outlet of the first chromatographic column 31, the second chromatographic column 32 and the third chromatographic column 33 are all arranged in a detachable structure, for example, the liquid inlet and the liquid outlet of the first chromatographic column 31, the second chromatographic column 32 and the third chromatographic column 33 are all provided with internal threads or are arranged on convex connecting pipes with external threads, and the liquid inlet and the liquid outlet at two ends of the first chromatographic column 31, the second chromatographic column 32 and the third chromatographic column 33 can be detachably connected in a threaded connection mode.

The first, second and third chromatography columns 31, 32 and 33 may each comprise six size columns of 1.8m, 1.6m, 1.4m, 1.2m, 1.0m and 0.8m. Of course, the first, second and third chromatography columns 31, 32, 33 may also comprise chromatography columns of more than 1.8m and less than 0.8m in order to meet the need for more product or feature product purification.

The first chromatographic sled 21, the second chromatographic sled 22 and the third chromatographic sled 23 at least comprise a driving pump 211 and a pipeline 212, and the first chromatographic sled 21, the second chromatographic sled 22 and the third chromatographic sled 23 are used as an integrated module, and other instruments and parts can be further included in the first chromatographic sled 21, the second chromatographic sled 22 and the third chromatographic sled 23. The drive pump 211 is used for driving the flow of the sample solution, and the drive pump 211 itself can also realize control of the flow rate and the flow velocity of the drive sample solution. A pipe 212 is connected between the drive pump 211 and the column, the pipe 212 being used to inject a sample solution into the column.

Wherein, the pipe 212 is detachably installed in each chromatographic sled, one end of the pipe 212 is detachably connected with the driving pump 211, and the other end of the pipe 212 is detachably connected with the first chromatographic column 31, the second chromatographic column 32 or the third chromatographic column 33. One end of the pipe 212 may be provided with a first connector 213, the other end of the pipe 212 may be provided with a second connector 214, and the first connector 213 and the second connector 214 may be liquid inlets and liquid outlets having different sizes, so that the pipe 212 with different calibers can be connected with the driving pump 211 and each color column. The first and second connectors 213 and 214 may be provided with external or internal threads at both ends, and the driving pump 211 and the respective chromatography columns may be provided with corresponding internal or external threads, and the pipe 212 may be detachably connected by the threaded connection of the first and second connectors 213 and 214.

The tubing 212 may comprise four dimensions of 2.0in, 1.5in, 1.0in, and 0.5 in. Of course, the tubing 212 may also include tubing greater than 2.0in and less than 0.5in order to meet the need for more product or feature product purification.

The first and second connection members 213 and 214 may also include four or more kinds, one end of the plurality of first connection members 213 may have the same size, and one end of the first connection member 213 is for detachable connection with the driving pump 211; the other ends of the plurality of first connectors 213 may have different sizes, and the other ends of the first connectors 213 may satisfy the detachable connection of four kinds of pipes 212 of 2.0in, 1.5in, 1.0in, and 0.5 in. The two ends of the plurality of second connectors 214 may have different sizes, one end of the second connector 214 may satisfy the detachable connection of 2.0in, 1.5in, 1.0in and 0.5in of four kinds of pipelines 212, and the other end of the second connector 214 may satisfy the detachable connection of 1.8m, 1.6m, 1.4m, 1.2m, 1.0m and 0.8m of six kinds of chromatographic columns. In other words, when it is necessary to replace the pipe 212 of a different size, it is necessary to replace the first connector 213 and the second connector 214 of corresponding sizes at the same time.

In other embodiments, the two ends of the tube 212 are provided with connection portions, and the connection portions of the tube 212 may have different connection calibers, so that the tube 212 can be detachably connected with the driving pump 211 and chromatographic columns of different sizes. This configuration requires that the same size tubing 212 be provided in plurality so that the same size tubing 212 can fit into different size columns.

In other embodiments, the driving pump 211 may be detachably fixed in each chromatographic skid, so that the driving pump 211 may be replaced and maintained, and the driving pump 211 with different flow rate and flow speed control may be replaced to meet the driving requirement.

In other embodiments, the driving pump 211 may be fixedly connected to the pipe 212, and the driving pump 211 and the pipe 212 form an integrated structure, and are detached and installed together, so as to realize replacement together, so as to meet the requirements of specific flow and flow speed driving, and meet the requirements of maintenance.

In this embodiment, the driving pump 211 may be provided with a plurality of driving gears, where the plurality of driving gears correspond to a plurality of pipes 212 with different sizes, for example, four driving gears are provided to correspond to the four pipes 212 with different sizes, and the different driving gears have different driving and flow rates, so as to provide the chromatographic skid with sample solutions with corresponding flow rates and flow rates in cooperation with the pipes 212 with different sizes.

In this embodiment, the first chromatographic skid 21, the second chromatographic skid 22 and the third chromatographic skid 23 may further include a flow valve and a flow meter, wherein the flow valve and the flow meter may be installed in the pipe 212, the flow valve is used for controlling the flow rate of the sample solution, and the flow meter is used for monitoring the flow rate and the flow velocity of the sample solution in the pipe 212. The flow valve and the flow meter may be installed at the position of the liquid outlet of the driving pump 211, and control of the flow rate and monitoring of the flow rate and the flow velocity may be realized. The drive pump 212 may cooperate with a flow valve and a flow meter to achieve control of flow and flow rate.

In other embodiments, one or more of the first, second, third and fourth liquid storage chambers 11, 12, 13 and 14 may be configured to be detachably mounted to facilitate replacement and maintenance of the first, second, third and fourth liquid storage chambers 11, 12, 13 and 14.

In other embodiments, the expandable chromatographic separation and purification device may not include one or more of the first liquid storage cavity 11, the second liquid storage cavity 12, the third liquid storage cavity 13 and the fourth liquid storage cavity 14, and the first liquid storage cavity 11, the second liquid storage cavity 12, the third liquid storage cavity 13 and the fourth liquid storage cavity 14 that are not included may be connected with the chromatographic separation and purification device in an external connection manner to form a complete chromatographic liquid path, and may also implement transition storage of the sample solution.

In this embodiment, the chromatographic separation and purification process of the expandable chromatographic separation and purification device can separate and remove unwanted substances. For example, product A (ProteinA), the antibody purification medium, is a Staphylococcus aureus cell wall protein that specifically binds to the Fc region of human and mammalian antibodies (primarily IgG). The affinity filler for antibody purification can be prepared by combining protein A with agarose gel in a certain mode. The expandable chromatographic separation and purification device is used for chromatography, and the steps of protein A chromatography and refined chromatography can remove viruses to a certain extent to obtain virus-free staphylococcus aureus cell wall proteins.

The expandable chromatographic separation and purification device in this embodiment will be described by taking chromatography for the above product a, product B, product C and product D as an example.

When the product A is subjected to chromatography (purification), a pipeline 212 with the size of 2.0in is arranged in the first chromatographic sled 21, and a first chromatographic column 31 with the size of 1.8m is arranged; a 1.5 in-sized tube 212 is installed in the second chromatographic skid 22, and a 1.0m second chromatographic column 32 is installed; a 1.0 in-sized tube 212 is installed in the third sled 23, and a 0.8m third chromatographic column 33 is installed; wherein, the first chromatographic column 31 with the diameter of 1.8m is used for purifying the product A for the first time, the second chromatographic column 32 with the diameter of 1.0m is used for purifying the product A for the second time, and the third chromatographic column 33 with the diameter of 0.8m is used for purifying the product A for the third time.

When the product B is subjected to chromatography (purification), a pipeline 212 with the size of 1.5in is arranged in the first chromatographic sled 21, and a first chromatographic column 31 with the size of 1.0m is arranged; a 1.5 in-sized tube 212 is installed in the second chromatographic skid 22, and a 1.0m second chromatographic column 32 is installed; a 1.0 in-sized tube 212 is installed in the third sled 23, and a 1.0m third chromatographic column 33 is installed; wherein, the first chromatographic column 31 with the diameter of 1.0m is used for purifying the product B for the first time, the second chromatographic column 32 with the diameter of 1.0m is used for purifying the product B for the second time, and the third chromatographic column 33 with the diameter of 1.0m is used for purifying the product B for the third time.

When the product C is subjected to chromatography (purification), a pipeline 212 with the size of 2.0in is arranged in the first chromatographic sled 21, and a first chromatographic column 31 with the size of 1.8m is arranged; a 2.0 in-sized tube 212 is installed in the second chromatographic skid 22, and a 1.4m second chromatographic column 32 is installed; a 2.0 in-sized tube 212 is installed in the third sled 23, and a 1.4m third chromatographic column 33 is installed; wherein, the first chromatographic column 31 with the diameter of 1.8m is used for purifying the product C for the first time, the second chromatographic column 32 with the diameter of 1.4m is used for purifying the product C for the second time, and the third chromatographic column 33 with the diameter of 1.4m is used for purifying the product C for the third time.

When the product D is subjected to chromatography (purification), a pipeline 212 with the size of 1.0in is arranged in the first chromatographic sled 21, and a first chromatographic column 31 with the size of 1.0m is arranged; a 1.0 in-sized tube 212 is installed in the second chromatographic skid 22, and a 1.0m second chromatographic column 32 is installed; a 1.0 in-sized tube 212 is installed in the third sled 23, and a 1.0m third chromatographic column 33 is installed; wherein, the first chromatographic column 31 with the diameter of 1.0m is used for purifying the product D for the first time, the second chromatographic column 32 with the diameter of 1.0m is used for purifying the product D for the second time, and the third chromatographic column 33 with the diameter of 1.0m is used for purifying the product D for the third time.

From the above, the expandable chromatographic separation and purification device of the embodiment can adapt to the flow and the flow rate required by different product chromatography by replacing the pipelines 212 with different sizes, so that the purchasing cost of the chromatographic pry is greatly reduced.

According to the expandable chromatographic separation and purification device, as the detachable pipeline 212 is arranged in the chromatographic sled, after the chromatographic column with the required size is selected for different samples, the chromatographic sled can be matched with the chromatographic column by replacing the pipeline 212 with the corresponding size, so that the flow and the flow velocity of the required sample are injected into the chromatographic column by the chromatographic sled, the whole chromatographic sled is not required to be replaced, the number of purchased chromatographic sleds is greatly reduced, and the cost is reduced; and the replaceable pipeline is convenient for maintenance of the chromatographic pry.

In an embodiment, a scalable chromatographic separation and purification device is provided, where the scalable chromatographic separation and purification device at least includes a chromatographic sled and a chromatographic column, where the chromatographic sled has the same structure as the chromatographic sled in any of the above embodiments, that is, a pipe in the chromatographic sled is detachably installed, and the chromatographic column with different sizes can be adapted by changing pipes with different sizes. The chromatographic column is also the same as the chromatographic column in any of the embodiments, and can be detachably arranged in the chromatographic separation and purification device, so that the requirements of different sample solution chromatography can be met by replacing the chromatographic columns with different sizes.

In this embodiment, the expandable chromatographic separation and purification device can select and set a required number of chromatographic pryes and chromatographic columns according to different chromatographic times. For example, when only a single chromatography is needed, a chromatographic sled and a chromatographic column are arranged in the expandable chromatographic separation and purification device; when 4 times of chromatography is needed, four chromatographic prys and four chromatographic columns are arranged in the expandable chromatographic separation and purification device. Of course, the expandable chromatographic separation and purification device can be correspondingly provided with a required number of liquid storage cavities.

The foregoing description of the utility model has been presented for purposes of illustration and description, and is not intended to be limiting. Several simple deductions, modifications or substitutions may also be made by a person skilled in the art to which the utility model pertains, based on the idea of the utility model.

Claims (10)

1. The expandable chromatographic separation and purification device is characterized by comprising a first liquid storage cavity, a first chromatographic sled, a first chromatographic column, a second liquid storage cavity, a second chromatographic sled, a second chromatographic column, a third liquid storage cavity, a third chromatographic sled, a third chromatographic column and a fourth liquid storage cavity which are sequentially communicated;

the first chromatographic sled is used for injecting a sample in the first liquid storage cavity into a first chromatographic column, and the first chromatographic column is used for carrying out first separation and purification on the sample and discharging the separated and purified sample into the second liquid storage cavity;

the second chromatographic sled is used for injecting the sample in the second liquid storage cavity into a second chromatographic column, and the second chromatographic column is used for carrying out second separation and purification on the sample and discharging the separated and purified sample into the third liquid storage cavity;

the third chromatographic sled is used for injecting the sample in the third liquid storage cavity into a third chromatographic column, and the third chromatographic column is used for separating and purifying the sample for the third time and discharging the separated and purified sample into the fourth liquid storage cavity;

the first chromatographic column, the second chromatographic column and the third chromatographic column are all detachably arranged in the chromatographic separation and purification device; the first chromatographic sled, the second chromatographic sled and the third chromatographic sled all include drive pump and demountable installation's pipeline at least, the drive pump passes through pipeline drive sample is poured into first chromatographic column, second chromatographic column or in the third chromatographic column, first chromatographic sled second chromatographic sled with third chromatographic sled is used for respectively through changing not equidimension the chromatographic column of pipeline adaptation response size.

2. The scalable chromatographic separation purification device of claim 1, wherein the conduit has dimensions of at least 2.0in, 1.5in, 1.0in, and 0.5in; and/or the dimensions of the first, second and third chromatography columns comprise at least 1.8m, 1.6m, 1.4m, 1.2m, 1.0m and 0.8m.

3. The scalable chromatographic separation purification device of claim 2, wherein the drive pump is provided with a plurality of drive stages, each having a different drive flow rate and flow velocity, each of the drive stages corresponding to a size of the conduit.

4. The expandable chromatographic separation purification device of claim 1, wherein one end of the conduit is detachably connected to the drive pump and the other end of the conduit is detachably connected to the first chromatographic column, the second chromatographic column, or the third chromatographic column.

5. The expandable chromatographic separation and purification device according to claim 4, wherein the two ends of the pipeline are provided with connecting parts, and the pipeline is detachably installed through the connecting parts.

6. The expandable chromatographic separation purification device of claim 4, wherein one end of the conduit is detachably connected to the drive pump by a first connector and the other end of the conduit is detachably connected to the first chromatographic column, the second chromatographic column, or the third chromatographic column by a second connector.

7. The expandable chromatographic separation purification device of claim 1, wherein the first chromatographic skid, the second chromatographic skid, and the third chromatographic skid each further comprise a flow valve and a flow meter disposed within the conduit.

8. The expandable chromatographic separation purification device of any of claims 1-7, wherein the drive pump is removably mounted within the first chromatographic sled, the second chromatographic sled, or the third chromatographic sled.

9. The expandable chromatographic separation purification device of claim 8, wherein the drive pump is fixedly connected to the conduit, the drive pump and the conduit for removal and installation together.

10. The expandable chromatographic separation and purification device is characterized by comprising a chromatographic column and a chromatographic sled;

the chromatographic sled is used for injecting a sample into a chromatographic column, and the chromatographic column is used for separating and purifying the sample;

the chromatographic column is detachably arranged in the chromatographic separation and purification device; the chromatographic sled at least comprises a driving pump and a pipeline which is detachably arranged, wherein the driving pump drives a sample to be injected into the chromatographic column through the pipeline, and the chromatographic sled is respectively used for adapting to the chromatographic column with the corresponding size through replacing the pipeline with different sizes.