CN220056786U

CN220056786U - PCR chip clamping device

Info

Publication number: CN220056786U
Application number: CN202321712727.3U
Authority: CN
Inventors: 吴梅娟
Original assignee: Shenzhen Borui Biotechnology Co ltd
Current assignee: Shenzhen Borui Biotechnology Co ltd
Priority date: 2023-06-30
Filing date: 2023-06-30
Publication date: 2023-11-21
Anticipated expiration: 2033-06-30

Abstract

The utility model discloses a PCR chip clamping device, comprising: the base is provided with a plurality of clamping grooves for placing chips and a plurality of positioning holes for fixing the limiting columns; the first pressing piece is arranged on the base and provided with a plurality of first hole sites corresponding to the clamping grooves, a first claw is arranged below the same side of the plurality of first hole sites, and a plurality of first limiting holes corresponding to the positioning holes and used for horizontally moving the limiting columns are formed in the first pressing piece; the second pressing piece is arranged on the first pressing piece and is provided with a plurality of second hole sites corresponding to the clamping grooves and the first hole sites, one sides of the second hole sites, which are far away from the first clamping jaws, are provided with second clamping jaws, and the second pressing piece is provided with a plurality of second limiting holes corresponding to the positioning holes and used for horizontally moving the limiting columns. The utility model can rapidly clamp the chip, and repeatedly operate the chip after clamping, and has the advantages of no need of disassembly and assembly, compact structure and simple operation.

Description

PCR chip clamping device

Technical Field

The utility model relates to the field of medical instruments, in particular to a PCR chip clamping device.

Background

Digital PCR (dPCR) is a nucleic acid molecule absolute quantitative technology, which is a high-sensitivity nucleic acid absolute quantitative analysis technology developed after Real-time fluorescent quantitative PCR (Real-time quantitative PCR, qPCR), and is realized by equally dividing a reaction system into a large number of independent micro-reaction units for PCR amplification and calculating the copy number of nucleic acid according to poisson distribution and positive proportion and matching with an optical detection device. Compared with the traditional PCR technology, the digital PCR technology does not depend on a standard curve, has higher sensitivity, accuracy and high tolerance, and can realize absolute quantitative analysis of samples. The technology can be applied to the fields of early screening and monitoring of tumors, genetic disease gene detection, pathogenic microorganism hypersensitive detection and the like.

The digital PCR needs to be matched with a microfluidic chip to carry out various detection, the microfluidic chip can carry out full-flow operations such as droplet generation, droplet storage and tiling, PCR thermal cycle, fluorescence imaging detection and the like, and the microfluidic chip has bending phenomenon after film sealing because of the whole thinness, so that the bonding degree of a PCR thermal cycle heating surface and the chip is influenced, and meanwhile, the fluorescence imaging detection precision is influenced. The existing chip compacting structures are inconvenient to take and place chips, and are complicated in clamping or require repeated clamping, so that other pollution phenomena such as oil leakage and sample leakage are extremely easy to occur.

Disclosure of Invention

The utility model aims to provide a PCR chip clamping device which aims to solve the problems of tedious and repeated clamping of the traditional chip clamping and the like.

Other features and advantages of the utility model will be apparent from the following detailed description, or may be learned by the practice of the utility model.

A PCR chip clamping device, comprising:

the base is provided with a plurality of clamping grooves for placing chips and a plurality of positioning holes for fixing the limiting columns;

the first pressing piece is arranged on the base and provided with a plurality of first hole sites corresponding to the clamping grooves, a first claw is arranged below the same side of the plurality of first hole sites, and a plurality of first limiting holes corresponding to the positioning holes and used for horizontally moving the limiting columns are formed in the first pressing piece;

the second pressing piece is arranged on the first pressing piece and is provided with a plurality of second hole sites corresponding to the clamping grooves and the first hole sites, one sides of the second hole sites, which are far away from the first clamping claws, are provided with second clamping claws, and the second pressing piece is provided with a plurality of second limiting holes corresponding to the positioning holes and used for horizontally moving the limiting columns;

after the first pressing piece and the second pressing piece are mounted on the base, the first clamping jaw and the second clamping jaw respectively support against two sides of the clamping groove.

Further, the locating hole is a threaded hole, and the limit post is installed on the locating hole in a threaded mode. When the locating hole adopts the screw hole, be convenient for compress tightly piece, second through spacing post and assemble first clamp, second clamp and base together fast, and be convenient for adjust the clamp force of first jack catch and second jack catch fast.

Further, the first limiting hole and the second limiting hole are kidney-shaped counter bores. The waist-shaped counter bore is adopted, so that the limit post can slide left and right after being inserted.

Further, one end of the first pressing piece and the other end of the second pressing piece are respectively provided with a first limiting block and a second limiting block, the first limiting block is arranged upwards, and the second limiting block is arranged downwards. The first limiting block and the second limiting block are adopted to limit the first pressing piece and the second pressing piece mutually, so that the first pressing piece and the second pressing piece are prevented from being excessively separated.

Further, one end of the first pressing piece and the other end of the second pressing piece are respectively provided with a first reset groove and a second reset groove which are concave. The first reset groove and the second reset groove are arranged, so that the first pressing piece and the second pressing piece can be conveniently separated or folded by manual operation.

Further, the limit column comprises a column body and a cover body formed at the top of the column body, and an elastic sheet is arranged below the cover body. Set up the shell fragment for have the space that floats between spacing post and the second compress tightly the piece, and then avoid first jack catch and second jack catch to compress tightly excessively to the chip, simultaneously, carry out thermal cycle work to the chip, can make the setting with the PCR thermal cycle heat-conducting plate of chip bottom can top to the chip bottom, keep more inseparable contact with the chip bottom, improve the heating effect.

Further, steps corresponding to the first clamping jaw and the second clamping jaw are arranged on two sides of the clamping groove. The step is arranged, and when the first clamping jaw and the second clamping jaw do not press the chip, the first clamping jaw and the second clamping jaw can be hidden on the step and can not influence the taking and placing of the chip.

Further, the clamping groove is hollow. The heat conducting plate which is convenient for heating the chip can be directly contacted with the bottom of the chip from the bottom of the base to heat the chip.

By adopting the scheme, the utility model has the beneficial effects that:

the clamping device can clamp a plurality of chips at one time, can clamp rapidly when clamping, has a simple structure, can be used for taking and placing rapidly, is simple to operate, does not influence the operation space on the chips, can continuously complete a plurality of working procedures after clamping once, and greatly improves the working efficiency of the digital PCR instrument.

The device can be repeatedly used after once clamping, does not need to be disassembled and assembled, and has compact structure and simple operation.

Drawings

FIG. 1 is a schematic diagram showing an exploded structure of a PCR chip clamping apparatus according to the present utility model;

FIG. 2 is a schematic view showing the structure of the PCR chip clamping apparatus in an unlocked state;

FIG. 3 is a partial cross-sectional view of the PCR chip clamping apparatus of the present utility model in an unlocked state;

FIG. 4 is a schematic view showing the structure of the PCR chip clamping device in a locked state;

FIG. 5 is a partial sectional view of the PCR chip clamping apparatus of the present utility model in a locked state;

FIG. 6 is a schematic view of a spacing post according to the present utility model;

FIG. 7 is a schematic diagram of the structure of a PCR chip of the present utility model;

FIG. 8 is a schematic diagram of the PCR chip clamping device of the present utility model mated with a thermal cover and a PCR thermal cycling heat conducting plate.

Wherein, the attached drawings mark and illustrate:

1. a base; 11. a clamping groove; 111. a step; 12. positioning holes; 2. a chip; 21. a liquid column; 22. a liquid pool; 3. a first clamping member; 31. a first hole site; 32. a first claw; 33. a first limiting hole; 34. a first limiting block; 35. a first reset groove; 4. a second clamping member; 41. a second hole site; 42. a second claw; 43. a second limiting hole; 44. a second limiting block; 45. a second reset groove; 5. a limit column; 51. a column body; 52. a cover body; 53. a spring plate; 6. PCR thermal cycle heat-conducting plate; 7. and a heat preservation cover.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It is noted that when an element is referred to as being "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or intervening elements may also be present. When the terms "one end," "the other end," "the first," "the second," and the like are used to distinguish similar objects from each other, and are not necessarily used to describe a particular order or sequence, it should be understood that the terms so used may be interchanged where appropriate.

Referring to FIG. 1, the present utility model provides a PCR chip clamping apparatus comprising:

the base 1, the base 1 has a plurality of clamping grooves 11 used for placing chips 2, and a plurality of positioning holes 12 used for fixing the limit posts 5;

the first pressing piece is arranged on the base 1 and is provided with a plurality of first hole sites 31 corresponding to the clamping grooves 11, a first claw 32 is arranged below the same side of the plurality of first hole sites 31, and a plurality of first limit holes 33 corresponding to the positioning holes 12 and used for horizontally moving the limit posts 5 are arranged on the first pressing piece;

the second pressing piece is arranged on the first pressing piece and is provided with a plurality of second hole sites 41 corresponding to the clamping grooves 11 and the first hole sites 31, one side, far away from the first clamping jaw 32, of the second hole sites 41 is provided with a second clamping jaw 42, and the second pressing piece is provided with a plurality of second limiting holes 43 corresponding to the positioning holes 12 and used for horizontally moving the limiting column 5;

wherein, after the first pressing member and the second pressing member are mounted on the base 1, the first claw 32 and the second claw 42 respectively abut against two sides of the clamping groove 11.

In use, as shown in fig. 2, when the pressing device is placed in the chip 2 but not locked, the limiting post 5 is located at the right side of the second limiting hole 43, so that the chip 2 can be freely taken and placed. The user can press the chip 2 by pushing the first pressing member and the second pressing member inwards by the left hand and the right hand respectively.

As shown in fig. 3, the second pressing member is located above the first pressing member, and when the pressing device is placed in the chip 2 but not locked, the second claw 42 of the second pressing member and the first claw 32 of the first pressing member are not pressed onto the chip 2, and at this time, the chip 2 can be freely taken and placed.

As shown in fig. 4, after the first pressing member and the second pressing member are folded, the first claw 32 and the second claw 42 are folded to position the chip 2, the pressing device locks the chip 2, and at this time, the limiting post 5 is located at the left side of the second limiting hole 43, and the chip 2 is pressed and fixed on the base 1.

As shown in fig. 5, the second jaw 42 of the second pressing member and the first jaw 32 of the first pressing member firmly press the chip 2, and at this time the chip 2 is pressed and fixed. If the chip 2 is to be taken out, the left and right hands push the second pressing member and the first pressing member outwards respectively, so that the first claw 32 and the second claw 42 are separated from the upper part of the chip 2, and the chip 2 can be taken out.

In an embodiment, referring to fig. 3 and 5, steps 111 corresponding to the first jaw 32 and the second jaw 42 are disposed on two sides of the slot 11. The step 111 is provided, and when the first jaw 32 and the second jaw 42 do not press the chip 2, the first jaw 32 and the second jaw 42 can be hidden on the step 111 without affecting the taking and placing of the chip 2.

In one embodiment, referring to FIG. 1, the slot 11 is hollow. The heat-conducting plate which is convenient for heating the chip 2 can be directly contacted with the bottom of the chip 2 from the bottom of the base 1 to heat the chip 2

In one embodiment, the positioning hole 12 is a threaded hole, and the limiting post 5 is mounted on the positioning hole 12 in a threaded manner. When the positioning hole 12 adopts a threaded hole, the first pressing member, the second pressing member and the base 1 are conveniently and quickly assembled together through the limit column 5, and the pressing forces of the first claw 32 and the second claw 42 are conveniently and quickly adjusted.

In one embodiment, the first and second limiting apertures 33, 43 are kidney-shaped counterbores. The waist-shaped counter bore is adopted, so that the limit post 5 can slide left and right after being inserted.

In an embodiment, please continue to refer to fig. 1, a first limiting block 34 and a second limiting block 44 are respectively disposed at one end of the first pressing member and the other end of the second pressing member, the first limiting block 34 is disposed upward, and the second limiting block 44 is disposed downward. The first limiting block 34 and the second limiting block 44 are adopted to limit the first pressing piece and the second pressing piece mutually, so that the first pressing piece and the second pressing piece are prevented from being excessively separated.

In an embodiment, referring to fig. 1, a first reset groove 35 and a second reset groove 45 are respectively disposed at one end of the first pressing member and the other end of the second pressing member. The first and second reset grooves 35 and 45 are provided to facilitate the manual operation of the first and second compressing members to be separated or folded.

In an embodiment, referring to fig. 6, the limiting post 5 includes a post body 51 and a cover 52 formed on the top of the post body 51, and a spring piece 53 is disposed below the cover 52. Set up shell fragment 53 for have the space that floats between spacing post 5 and the second compress tightly the piece, and then avoid first jack catch 32 and second jack catch 42 to compress tightly excessively to chip 2, simultaneously, carry out thermal cycle work to chip 2, can make the setting with the PCR thermal cycle heat-conducting plate 6 of chip 2 bottom can be pushed up to chip 2 bottom, keep more inseparable contact with chip 2 bottom, improve the heating effect.

In an embodiment, the second pressing member is provided with six second limiting holes 43, the corresponding first pressing member is provided with six first limiting holes 33, the corresponding base 1 is provided with six positioning holes 12, when the first pressing member, the second pressing member and the base 1 are aligned in a superposition manner, the positioning holes 12 on the base 1 below can be seen through the alignment of the first limiting holes 33 and the second limiting holes 43, the limiting columns 5 can also comprise movable gaskets, when the second pressing member is assembled, the gaskets are sequentially placed in the second limiting of the second pressing member, then the elastic sheets 53 are sequentially placed on the gaskets, the limiting columns 5 are sequentially taken to penetrate the elastic sheets 53 and the gaskets, the positioning holes 12 of the base 1 are aligned, and the second pressing member and the first pressing member are fixed on the base 1.

Working principle:

in specific use, as shown in fig. 1-7, the pressing device locks the chip 2, and the limiting post 5 is located at the left side of the second limiting hole 43, so that the chip 2 is pressed and fixed on the base 1. The elastic sheet 53 firmly presses the second clamping jaw 42 and the first clamping jaw 32 against the chip 2, at this time, the chip 2 is pressed and fixed, and the surfaces of the liquid column 21 and the liquid pool 22 on the chip 2 are not covered. The subsequent liquid drop generation, PCR thermal cycle and optical photographing detection can be directly carried out, and repeated clamping is not needed. If the chip 2 is to be taken out, the left and right thumbs respectively scratch the first reset groove 35 and the second reset groove 45 and push the second pressing member and the first pressing member to the outside, so that the first claw 32 and the second claw 42 are separated from the chip 2, and the chip 2 can be taken out.

As shown in fig. 8, the digital PCR needs to perform thermal cycle, the PCR thermal cycle heat conducting plate 6 is located under the chip 2, and rises to be close to the bottom surface of the chip 2 when performing thermal cycle, so as to be better attached to the chip 2, and then lifts the chip 2 upwards by a distance, such as 0.5mm, at this time, the second pressing member and the first pressing member will be lifted up by 0.5mm at the same time.

The heat preservation cover 7 presses down the position of the liquid pool of the cover chip 2 to preserve heat, at the moment, the elastic sheet 53 is further compressed, after the PCR thermal cycle is finished, the heat preservation cover 7 ascends to reset, the PCR thermal cycle heat-conducting plate 6 descends to reset, the elastic sheet 53 presses the second pressing piece and the first pressing piece back to the base 1, and the elastic sheet 53 is in an initial state.

The clamping device disclosed by the embodiment can clamp a plurality of chips 2 at one time, can realize rapid clamping during clamping, has a simple structure, can be rapidly taken and put, is simple to operate, does not influence the operation space on the chips 2, can continuously complete a plurality of working procedures after once clamping, and greatly improves the working efficiency of the digital PCR instrument.

Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present utility model, and are not limiting; although the utility model has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present utility model.

Claims

1. A PCR chip clamping device, comprising:

2. The PCR chip clamping apparatus as claimed in claim 1, wherein the positioning hole is a threaded hole, and the limit post is threadedly mounted on the positioning hole.

3. The PCR chip clamping apparatus as claimed in claim 1, wherein the first and second limiting apertures are kidney-shaped counterbores.

4. The PCR chip clamping apparatus as in claim 1, wherein a first stopper and a second stopper are disposed at one end of the first pressing member and the other end of the second pressing member, respectively, the first stopper being disposed upward, and the second stopper being disposed downward.

5. The PCR chip clamping apparatus as claimed in claim 1, wherein the first compression member has one end and the second compression member has the other end provided with a first reset groove and a second reset groove, respectively, which are recessed.

6. The PCR chip clamping apparatus as in claim 1, wherein the limit post includes a post body and a cover body formed on a top of the post body, and a spring plate is provided under the cover body.

7. The PCR chip clamping apparatus as claimed in claim 1, wherein the clamping groove is provided with steps on both sides thereof corresponding to the first and second jaws.

8. The PCR chip clamping apparatus in accordance with claim 1, wherein the clamping slot is hollow.