CN219027382U

CN219027382U - 96-linkage PCR plate pressure cover die

Info

Publication number: CN219027382U
Application number: CN202223329264.0U
Authority: CN
Inventors: 任力; 刘子贤; 袁义勇
Original assignee: Meidong Huicheng Life Technology Kunshan Co ltd
Current assignee: Meidong Huicheng Life Technology Kunshan Co ltd
Priority date: 2022-12-13
Filing date: 2022-12-13
Publication date: 2023-05-16
Anticipated expiration: 2032-12-13

Abstract

The utility model relates to the technical field of laboratory appliances, in particular to a 96-linkage PCR plate gland die which comprises a base and a lower die, wherein a placing groove is formed in the base, the lower die is arranged in the placing groove, adjusting mechanisms are arranged on the left side and the right side of the placing groove, a control module is arranged on the back surface of the placing groove, and a gland mechanism is arranged above the placing groove; the gland mechanism comprises an upper die, a fixed plate, a fixed rod and a telescopic cylinder, wherein the upper die is arranged above the placing groove, the fixed plate is arranged above the upper die, the upper die is connected with the fixed plate through the fixed rod, and the telescopic cylinder is arranged in the center of the top of the fixed plate.

Description

96-linkage PCR plate pressure cover die

Technical Field

The utility model relates to the technical field of laboratory tools, in particular to a 96-linkage PCR plate gland die.

Background

A96-well PCR plate is a 96-well PCR plate, which is mainly used as a carrier of a primer, taq DNA polymerase, dNTP, template nucleic acid, mg, buffer solution and the like participating in amplification reaction in polymerase chain reaction (Polymerase Chain Reaction, PCR), and when a PCR experiment is carried out, before the sample is added to the PCR plate and the PCR plate is ready to be put into a PCR instrument for carrying out cyclic temperature rise and fall reaction, a cover of a plate hole for loading the sample needs to be closed by using a PCR optical flat cover.

At present, the sealing cover of the hole of the PCR plate after sample loading is usually finished by manual operation, however, because the PCR optical flat cover is of a plastic structure, and the size is small, the material is soft, the manual operation is high in requirement, high in difficulty and low in efficiency, in addition, the hole of the PCR plate is easy to pollute by the manual operation, the detection result is false positive or false negative, and the sealing cover is easy to cause hand ache after the manual operation for a long time, so that the sealing performance of the hole of the PCR sample loading is not guaranteed due to uneven pressing force channel, and the risk of bulge leakage is easy to occur;

therefore, it is important to design a novel 96-link PCR plate cap mold to solve the above-mentioned drawbacks.

Disclosure of Invention

The utility model aims to provide a 96-link PCR plate gland die for solving the problems in the background technology.

In order to achieve the above purpose, the present utility model provides the following technical solutions:

the 96-linkage PCR plate gland die comprises a base and a lower die, wherein a placing groove is formed in the base, the lower die is arranged in the placing groove, adjusting mechanisms are arranged on the left side and the right side of the placing groove, a control module is arranged on the back of the placing groove, and a gland mechanism is arranged above the placing groove;

the gland mechanism comprises an upper die, a fixing plate, a fixing rod and a telescopic cylinder, wherein the upper die is arranged above the placing groove, the fixing plate is arranged above the upper die, the upper die is connected with the fixing plate through the fixing rod, and the telescopic cylinder is arranged at the center of the top of the fixing plate.

As a preferable scheme of the utility model, positioning step surfaces are arranged on the periphery of the top of the lower die, a plurality of groups of circular grooves are arranged on the top of the lower die, positioning holes are arranged at four corners of the top of the lower die, and the front surface of the lower die is in threaded connection with a handle.

As a preferable scheme of the utility model, the adjusting mechanism comprises an adjusting box, the left side and the right side of the placing groove are respectively provided with the adjusting box, two groups of telescopic rods are arranged in the adjusting box, and the output ends of the two groups of telescopic rods are provided with positioning plates.

As a preferable scheme of the utility model, a positioning groove is formed in the inner side of the upper die, the inner size of the positioning groove is matched with that of the lower die, and a positioning column is arranged at a position corresponding to the lower die in the inner part of the positioning groove.

As a preferable scheme of the utility model, the fixing plate is fixedly connected with the base through four groups of supporting legs, the four groups of fixing rods are arranged, the four groups of fixing rods are respectively positioned at four corners of the top of the upper die, one end of each fixing rod is fixedly connected with the upper die, the other end of each fixing rod is in sliding connection with the fixing plate, and the output end of each telescopic cylinder is fixedly connected with the top of the upper die.

As a preferable scheme of the utility model, the left side and the right side of the front face of the control module are respectively provided with a prop, photoelectric sensors are arranged between the two groups of prop and on the front face of the control module, and the control module is electrically connected with the adjusting mechanism, the gland mechanism and the photoelectric sensors.

Compared with the prior art, the utility model has the beneficial effects that:

1. according to the utility model, through the matching design of the control module and the adjusting mechanism, when the PCR plate mounting template hole is capped, the PCR plate is firstly placed on the lower die, then the PCR optical flat cover is covered on the PCR plate mounting template hole, then the PCR plate is directly stretched into the placing groove through the lower die, the back surface of the lower die is propped by the prop after the lower die enters the placing groove, then the photoelectric sensor senses that the lower die reaches a designated position, the adjusting mechanism is controlled to adjust the position of the lower die towards the middle of the placing groove, then the left side and the right side of the lower die are clamped and fixed, the lower die is automatically aligned with the gland mechanism, then the gland mechanism is controlled by the control module to gland, the capping can be automatically completed without key operation, the operation is convenient and efficient, and the capping efficiency of the hole of the PCR plate is greatly improved.

2. According to the utility model, through the design of the lower die and the capping mechanism, when the capping mechanism caps, the upper die and the lower die are accurately aligned, so that the pressure is ensured to be uniform and stable, a plurality of PCR plate holes can be capped at one time or a small number of PCR plate holes can be capped, the problems of low efficiency of manual capping and easiness in bulge and liquid leakage are perfectly solved, and the experimental requirements of different sample numbers are met.

Drawings

FIG. 1 is a schematic diagram of the overall structure of the present utility model;

FIG. 2 is a schematic view of the internal structure of the base of the present utility model;

FIG. 3 is a schematic view of the lower die structure of the present utility model;

fig. 4 is a schematic view of the inner side structure of the upper mold of the present utility model.

In the figure: 1. a base; 2. a lower die; 3. a placement groove; 4. an adjusting mechanism; 5. a control module; 6. a capping mechanism; 7. an upper die; 8. a fixing plate; 9. a fixed rod; 10. a telescopic cylinder; 11. a grip; 12. an adjusting box; 13. a telescopic rod; 14. a positioning plate; 15. a positioning groove; 16. positioning columns; 17. abutting the column; 18. a photoelectric sensor.

Detailed Description

The technical solutions of the embodiments of the present utility model will be clearly and completely described below in conjunction with the embodiments of the present utility model, and it is apparent that the described embodiments are only some embodiments of the present utility model, but not all embodiments, and all other embodiments obtained by those skilled in the art without making any inventive effort based on the embodiments of the present utility model are within the scope of protection of the present utility model.

Examples

The embodiment provides a 96 allies oneself with PCR board gland mould, and this mould can play fine fixed action when the hole closing cap of PCR board, has increased closing cap stability simultaneously, has improved the whole convenience of mould.

Referring to fig. 1-4, the present utility model provides a technical solution:

the 96-linkage PCR plate gland die comprises a base 1 and a lower die 2, wherein a placing groove 3 is formed in the base 1, the lower die 2 is arranged in the placing groove 3, adjusting mechanisms 4 are arranged on the left side and the right side of the placing groove 3, a control module 5 is arranged on the back of the placing groove 3, and a gland mechanism 6 is arranged above the placing groove 3;

the gland mechanism 6 includes mould 7, fixed plate 8, dead lever 9 and flexible cylinder 10, and the top of standing groove 3 is provided with mould 7, and the top of mould 7 is provided with fixed plate 8, and mould 7 is connected with fixed plate 8 through dead lever 9, and the center department at fixed plate 8 top is provided with flexible cylinder 10.

In this embodiment, the implementation scenario specifically includes: after a PCR plate is placed on a lower die 2, a plate hole filled with a sample is covered with a PCR optical flat cover, then the lower die 2 is stretched into a placing groove 3, after a control module 5 senses that the lower die 2 reaches a specified position, an adjusting mechanism 4 is controlled to adjust the position of the lower die 2 to be aligned with an upper gland mechanism 6, then the left side and the right side of the lower die 2 are fixed, then a control module 5 controls a telescopic cylinder 10 at the top of a fixed plate 8 to press down an upper die 7, a fixed rod 9 is matched with the upper die 7 to stably press down, so that the upper die 7 and the lower die 2 are closed to compress the PCR optical flat cover to finish sealing, the telescopic cylinder 10 returns after sealing is finished, and then the lower die 2 is taken out.

Firstly, referring to fig. 1-4, positioning step surfaces are formed around the top of a lower die 2, a plurality of groups of circular grooves are formed in the top of the lower die 2, positioning holes are formed in four corners of the top of the lower die 2, a handle 11 is connected to the front of the lower die 2 in a threaded manner, a PCR plate is aligned to the circular grooves to place the PCR plate on the lower die 2, then a PCR optical flat cover is covered on a plate hole of a PCR plate mounting sample, and then the handle 11 is held to extend the lower die 2 into the placing groove 3;

in this embodiment, the specific structure of the adjusting mechanism 4 is as follows:

the adjusting mechanism 4 comprises an adjusting box 12, the left side and the right side of the placing groove 3 are respectively provided with the adjusting box 12, two groups of telescopic rods 13 are arranged in the adjusting box 12, and the output ends of the two groups of telescopic rods 13 are provided with positioning plates 14;

further, the left side and the right side of the front face of the control module 5 are respectively provided with a supporting column 17, the front face of the control module 5 is provided with a photoelectric sensor 18 between the two groups of supporting columns 17, the control module 5 is electrically connected with the adjusting mechanism 4, the gland mechanism 6 and the photoelectric sensor 18, the back face of the lower die 2 is supported by the supporting columns 17 after the lower die 2 enters the placing groove 3, then the photoelectric sensor 18 senses that the lower die 2 reaches a designated position, the telescopic rod 13 is controlled to push the positioning plate 14, the position of the lower die 2 is adjusted towards the middle of the placing groove 3, then the left side and the right side of the lower die 2 are clamped and fixed, and the lower die 2 and the gland mechanism 6 are automatically aligned;

then, the fixing plate 8 is fixedly connected with the base 1 through four groups of support legs, four groups of fixing rods 9 are arranged, the four groups of fixing rods 9 are respectively positioned at four corners at the top of the upper die 7, one end of each fixing rod 9 is fixedly connected with the upper die 7, the other end of each fixing rod 9 is in sliding connection with the fixing plate 8, the output end of the telescopic cylinder 10 is fixedly connected with the top of the upper die 7, after the lower die 2 is fixed, the telescopic cylinder 10 is controlled by the control module 5 to press the upper die 7 downwards, and the fixing rods 9 are matched with the upper die 7 to stably press downwards, so that the upper die 7 is closed with the lower die 2;

finally, the inside of last mould 7 has seted up constant head tank 15, and the inside size and the lower mould 2 looks adaptation of constant head tank 15 use, and the inside of constant head tank 15 is provided with reference column 16 with the position department that lower mould 2 corresponds, and the inside of constant head tank 15 is gone into to the top of lower mould 2 after last mould 7 and lower mould 2 are closed, and reference column 16 intubate the locating hole simultaneously and fix a position for the pressure stability that last mould 7 pushed down can not take place the skew, and flexible cylinder 10 return after the closing cap is accomplished, later take out lower mould 2 can.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a 96 allies oneself with PCR board gland mould, includes base (1) and bed die (2), its characterized in that: a placing groove (3) is formed in the base (1), a lower die (2) is arranged in the placing groove (3), adjusting mechanisms (4) are arranged on the left side and the right side of the placing groove (3), a control module (5) is arranged on the back of the placing groove (3), and a gland mechanism (6) is arranged above the placing groove (3);

the gland mechanism (6) comprises an upper die (7), a fixing plate (8), a fixing rod (9) and a telescopic cylinder (10), wherein the upper die (7) is arranged above the placing groove (3), the fixing plate (8) is arranged above the upper die (7), the upper die (7) is connected with the fixing plate (8) through the fixing rod (9), and the telescopic cylinder (10) is arranged at the center of the top of the fixing plate (8).

2. The 96-gang PCR plate capping die as claimed in claim 1, wherein: the positioning step faces are formed in the periphery of the top of the lower die (2), a plurality of groups of circular grooves are formed in the top of the lower die (2), positioning holes are formed in four corners of the top of the lower die (2), and the front face of the lower die (2) is connected with a grip (11) in a threaded mode.

3. The 96-gang PCR plate capping die as claimed in claim 1, wherein: the adjusting mechanism (4) comprises an adjusting box (12), the left side and the right side of the placing groove (3) are respectively provided with the adjusting box (12), two groups of telescopic rods (13) are arranged in the adjusting box (12), and the output ends of the two groups of telescopic rods (13) are provided with positioning plates (14).

4. The 96-gang PCR plate capping die as claimed in claim 1, wherein: the inner side of the upper die (7) is provided with a positioning groove (15), the inner size of the positioning groove (15) is matched with that of the lower die (2), and a positioning column (16) is arranged at the position of the inner part of the positioning groove (15) corresponding to the lower die (2).

5. The 96-gang PCR plate capping die as claimed in claim 1, wherein: the fixing plate (8) is fixedly connected with the base (1) through four groups of supporting legs, the fixing rods (9) are provided with four groups, the four groups of fixing rods (9) are respectively located at four corners of the top of the upper die (7), one end of each fixing rod (9) is fixedly connected with the upper die (7), the other end of each fixing rod (9) is slidably connected with the fixing plate (8), and the output end of each telescopic cylinder (10) is fixedly connected with the top of the upper die (7).

6. The 96-gang PCR plate capping die as claimed in claim 1, wherein: the control module (5) is characterized in that supporting columns (17) are arranged on the left side and the right side of the front face of the control module (5), photoelectric sensors (18) are arranged between the two groups of supporting columns (17), and the control module (5) is electrically connected with the adjusting mechanism (4), the gland mechanism (6) and the photoelectric sensors (18).