CN220019109U - Centrifugal dry-type chromosome banding equipment - Google Patents
Centrifugal dry-type chromosome banding equipment Download PDFInfo
- Publication number
- CN220019109U CN220019109U CN202320877535.1U CN202320877535U CN220019109U CN 220019109 U CN220019109 U CN 220019109U CN 202320877535 U CN202320877535 U CN 202320877535U CN 220019109 U CN220019109 U CN 220019109U
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- slide
- processing cavity
- turntable
- glass
- placing
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- 210000000349 chromosome Anatomy 0.000 title claims abstract description 23
- 239000011521 glass Substances 0.000 claims abstract description 57
- 239000007788 liquid Substances 0.000 claims abstract description 51
- 238000012545 processing Methods 0.000 claims abstract description 41
- 239000007921 spray Substances 0.000 claims abstract description 28
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 20
- 238000005507 spraying Methods 0.000 claims abstract description 9
- 230000005540 biological transmission Effects 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 238000001035 drying Methods 0.000 abstract description 18
- 238000000034 method Methods 0.000 abstract description 14
- 238000011161 development Methods 0.000 abstract description 5
- 210000004027 cell Anatomy 0.000 description 6
- 238000004043 dyeing Methods 0.000 description 6
- 238000011010 flushing procedure Methods 0.000 description 6
- 108010019160 Pancreatin Proteins 0.000 description 5
- 229940055695 pancreatin Drugs 0.000 description 5
- 239000002504 physiological saline solution Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000012550 audit Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 230000002559 cytogenic effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000007602 hot air drying Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
- Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics
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- Sampling And Sample Adjustment (AREA)
Abstract
The present utility model relates to the field of biological treatment devices. The centrifugal dry-type chromosome banding device comprises a processing cavity, wherein the upper end of the processing cavity is provided with an opening, and the top of a processor is connected with a cover body; the device also comprises a slice placing turntable, wherein at least ten slide glass placing grooves which are circumferentially distributed are arranged on the slice placing turntable; the liquid supply mechanism comprises a liquid storage bottle for storing the reagent and a spray head, the spray head is arranged on the inner wall of the processing cavity, the liquid storage bottle is positioned on the outer side of the processing cavity, the liquid storage bottle is connected with the spray head through a connecting pipeline, and the spray head is used for spraying the reagent on a glass slide of the glass slide placing groove; the bottom of the treatment cavity is provided with a liquid outlet. Compared with a drying mode, the method greatly improves the uniformity of the surface drying of the glass slide. Meanwhile, the time consumed by transferring the traditional reagent pools is avoided, and the sample processing speed is greatly improved. The steps of slide glass tape development are integrated in one processing cavity, so that the occupied space required by equipment is greatly saved.
Description
Technical Field
The utility model relates to the field of biological treatment equipment, in particular to chromosome banding equipment.
Background
In the process of cell inspection and analysis, a cell specimen such as a chromosome needs to be prepared on the surface of a glass slide so as to analyze and audit various characteristic indexes of the cell specimen by using a microscope, thereby obtaining an auxiliary diagnosis conclusion. In the preparation of such blood cell samples, it is necessary to take and stain the cells on the slide. The cytogenetic laboratory typically uses standard flow manual or preprogrammed automated equipment to strip and stain the cell specimens on the slides. The method comprises the steps of attaching a cell specimen such as a chromosome to the surface of a glass slide, then using a pancreatin tape and a dye liquor reagent for dip dyeing, and then, generating alternate bright and dark stripes on the stripes in the chromosome, thereby identifying each chromosome, and finding out structural stripe variation and abnormal quantity on each chromosome to obtain a required medical diagnosis result.
The traditional chromosome tape development is carried out, and after a worker places the slide in sequence in a storage pool of different reagents, the slide is washed with water and dried with water, and the slide is transported manually in the mode, so that the method is complicated.
There is also an automatic tape-developing device, for example, a full-automatic film dyeing apparatus with a drying function disclosed in CN213903057U includes a workbench, and a pancreatin liquid pool, a physiological saline pool, a flushing agent pool, a dyeing liquid pool and a flushing water pool which are arranged on the workbench. The automatic slice dyeing instrument also comprises a drying pool, a pancreatin liquid pool, a physiological saline pool, a flushing agent pool, a dyeing liquid pool, a flushing water pool and a drying Chi Shunci which are arranged side by side to form an operation pool group, wherein a moving track is arranged at one side of the operation pool group in parallel, and a manipulator device is arranged on the moving track; the automatic film dyeing instrument is also provided with a control device, and the manipulator device can move horizontally and longitudinally under the control of the control device.
The defects are that:
1) The manipulator device needs to move horizontally and longitudinally, a two-dimensional movement module is needed, two sets of driving motors are needed, and cost is high.
2) The drying pool and the reagent pool are independently arranged, so that the occupied space required by the device is increased, and meanwhile, the transfer time of transferring the extra glass slide between the reagent pool and the drying pool is also required.
3) The drying pond adopts the fan to blow the air of heating, realizes the stoving of slide glass, can't guarantee the stoving uniformity of all slide glass on the slide basket frame.
Disclosure of Invention
Aiming at the problems existing in the prior art, the utility model provides centrifugal dry-type chromosome banding equipment, which can solve at least one technical problem.
In order to achieve the above purpose, the utility model provides centrifugal dry-type chromosome banding equipment, which comprises a shell, wherein a processing cavity with an opening at the upper end is arranged in the shell, and is characterized in that a cover plate for covering the top of the processing cavity is connected with the shell;
the device also comprises a slice placing turntable, wherein the slice placing turntable is arranged in the processing cavity, and at least ten slide placing grooves which are circumferentially distributed are formed in the slice placing turntable;
the device also comprises a driving mechanism for driving the sheet placing turntable to rotate, wherein the driving mechanism is arranged in the shell and is in transmission connection with the sheet placing turntable;
the device comprises a processing cavity, a liquid storage bottle, a liquid supply mechanism and a spray head, wherein the liquid storage bottle is used for storing a reagent, the spray head is arranged on the inner wall of the processing cavity, the liquid storage bottle is positioned on the outer side of the processing cavity, the liquid storage bottle is connected with the spray head through a connecting pipeline, and the spray head is used for spraying the reagent on a glass slide of a glass slide placing groove;
and a liquid outlet is formed in the bottom of the treatment cavity.
According to the utility model, through optimizing the structure of the tape developing device, the spray heads of different reagents are sequentially arranged in the circumferential movement process of the slide glass on the slide placing turntable, and then after the reagents required for tape development are sprayed on the surface of the slide glass, the slide glass is rotated at a high speed by the slide placing turntable, so that the centrifugal spin-drying of liquid on the slide glass is realized. Compared with the drying mode, the uniformity of the surface drying of the glass slide is greatly improved. Meanwhile, the time consumed by transferring the traditional reagent pools is avoided, and the sample processing speed is greatly improved.
The utility model realizes the integration of each step of slide glass tape display in one processing cavity, thereby greatly saving the occupied space required by equipment.
Further preferably, an annular groove is formed in the outer side of the bottom of the processing cavity, and the drain port is formed in the bottom of the annular groove.
The liquid which is thrown out centrifugally is conveniently collected and discharged.
Further preferably, an auxiliary discharge port is formed in a side wall of the processing chamber, and the auxiliary discharge port is located in a region between the liquid discharge port and a position where the sheet placing turntable is located.
Through the arrangement of the auxiliary discharge port, the risk that discharged liquid is contacted with the glass slide due to untimely liquid discharge of the liquid discharge port is avoided.
Further preferably, a handle is detachably connected to the center of the sheet placing turntable.
Is convenient to take.
Further preferably, the bottom of the slide placement groove is provided with at least three protrusions, and the height of the protrusions is not more than 2mm.
Further preferably, two corners of the radial outer side of the slide glass placing groove are respectively provided with an arc-shaped notch with a section with a central angle larger than 180 degrees, and the notch is used for accommodating the corner of the slide glass.
Stress damage to the slide corners is avoided.
Further preferably, the slide placement groove comprises an inner zone and an outer zone arranged inside and outside, wherein the radial length of the inner zone is less than 1/2 of the radial length of the outer zone;
the depth of the inner zone is greater than the depth of the outer zone;
the bottom of the inner zone is connected with the bottom of the outer zone through an inclined plane which is inclined upwards from inside to outside.
The edge of the glass slide is lifted and tilted conveniently through the glass slide positioned in the inner region, and then the glass slide is taken out from the glass slide placing groove.
Further preferably, the slide placing turntable comprises an extending branch part extending outwards in the radial direction, and the slide placing groove is formed in the extending branch part.
The dead weight of the slice placing turntable is convenient to lighten, and the rotation is convenient. Meanwhile, liquid which is convenient to centrifugally throw out is thrown out from a gap between adjacent extension branch parts.
Further preferably, the driving mechanism comprises a driving motor, and a driving gear is arranged on a power output shaft of the driving motor;
the center of the sheet placing turntable is connected with a rotating shaft, a driven gear is arranged on the rotating shaft, and the driving gear is connected with the driven gear through a driving belt;
the rotating shaft is rotatably arranged on a base through a bearing, and the base is arranged in a region below the processing cavity in the shell;
the driving motor is mounted on the base in a suspending mode.
Further preferably, the center of the processing cavity is provided with an upward bulge part, and the center of the top of the bulge part is provided with an opening penetrating through a rotating shaft for driving the sheet placing turntable to rotate;
the top of the rotating shaft is detachably connected with a bracket, and the bottom of the center of the sheet placing turntable is provided with a slot for inserting the bracket.
The beneficial effects are that:
1) The movement of the glass slide is realized through a single driving motor, so that reagent spraying stations with different glass slide paths are realized. And centrifugal drying of the glass slide can be realized.
2) The occupied space of the device is controlled.
3) The time of slide transport is reduced.
4) Compared with a hot air drying mode, the drying uniformity of the glass slide is improved through a centrifugal drying mode.
Drawings
FIG. 1 is a schematic view of a partial structure of the present utility model;
FIG. 2 is a schematic diagram of the structure of the present utility model;
FIG. 3 is a schematic view of a partial structure of the present utility model;
FIG. 4 is a schematic view of a partial structure of the present utility model;
FIG. 5 is a schematic view of a partial structure of the present utility model;
FIG. 6 is a schematic view of a disc changer according to the present utility model;
FIG. 7 is a schematic view of a disc changer according to the present utility model;
fig. 8 is a schematic view of the structure of the processing chamber of the present utility model.
Wherein: 1 is a sheet placing turntable, 2 is a processing cavity, 3 is a spray head, 4 is a driving motor, 5 is a liquid outlet, 6 is an auxiliary liquid outlet, 7 is a rotating shaft, 8 is a shell, 9 is a bulge, 10 is a bracket, 11 is an inner zone, and 12 is an outer zone.
Detailed Description
The utility model is further described below with reference to the accompanying drawings.
Referring to fig. 1 to 8, in embodiment 1, a centrifugal dry-type chromosome banding apparatus includes a housing 8, a processing chamber 2 having an upper end opened is provided in the housing 8, and a cover plate for covering a top of the processing chamber 2 is connected to the housing 8; the device also comprises a slice placing turntable 1, wherein the slice placing turntable 1 is arranged in the processing cavity 2, and at least ten slide placing grooves which are circumferentially distributed are arranged on the slice placing turntable 1; the device also comprises a driving mechanism for driving the sheet placing turntable 1 to rotate, wherein the driving mechanism is arranged in the shell 8 and is in transmission connection with the sheet placing turntable 1; the device also comprises a liquid supply mechanism, wherein the liquid supply mechanism comprises a liquid storage bottle for storing the reagent and a spray head 3, the spray head 3 is arranged on the inner wall of the processing cavity 2, the liquid storage bottle is positioned on the outer side of the processing cavity 2, the liquid storage bottle is connected with the spray head 3 through a connecting pipeline, and the spray head 3 is used for spraying the reagent on a glass slide of the glass slide placing groove; the bottom of the treatment cavity 2 is provided with a liquid outlet 5. According to the utility model, through optimizing the structure of the tape developing device, the spray heads 3 of different reagents are sequentially arranged in the circumferential movement process of the slide glass on the slide placing turntable 1, so that after the reagents required for tape development are sprayed on the surface of the slide glass, the slide glass is rotated at a high speed through the slide placing turntable 1, and the centrifugal spin-drying of liquid on the slide glass is realized. Compared with the drying mode, the uniformity of the surface drying of the glass slide is greatly improved. Meanwhile, the time consumed by transferring the traditional reagent pools is avoided, and the sample processing speed is greatly improved. The utility model realizes that each step of slide glass tape development is integrated in one processing cavity 2, thereby greatly saving the occupied space required by equipment.
An annular groove is arranged on the outer side of the bottom of the processing cavity 2, and a liquid outlet 5 is arranged at the bottom of the annular groove. The liquid which is thrown out centrifugally is conveniently collected and discharged.
An auxiliary discharge port 6 is formed in the side wall of the processing cavity 2, and the auxiliary discharge port 6 is positioned in a region between the liquid discharge port 5 and the position of the sheet placing turntable 1. By the arrangement of the auxiliary discharge port 6, the risk that discharged liquid is contacted with the glass slide due to untimely liquid discharge of the liquid discharge port 5 is avoided. At least six spray head installation positions are arranged on the side wall of the treatment cavity, and the spray head installation positions are detachably connected with the spray heads. And an electromagnetic valve is arranged on a connecting pipeline between the spray head and the liquid storage bottle. The electromagnetic valve is connected with the control system, and the control system is connected with a sensor for sensing the rotation condition of the sheet placing turntable.
The center of the tablet placing turntable 1 is detachably connected with a handle. Is convenient to take.
At least three protrusions are arranged at the bottom of the glass slide placing groove, and the heights of the protrusions are not more than 2mm.
Two corners of the radial outer side of the slide glass placing groove are respectively provided with an arc-shaped notch with a section with a central angle larger than 180 degrees, and the notch is used for accommodating the corner of the slide glass. Stress damage to the slide corners is avoided.
The slide glass placing groove comprises an inner zone 11 and an outer zone 12 which are arranged inside and outside, wherein the radial length of the inner zone 11 is less than 1/2 of the radial length of the outer zone 12; the depth of the inner zone 11 is greater than the depth of the outer zone 12; the bottom of the inner zone 11 is connected to the bottom of the outer zone 12 by an inclined surface inclined upward from inside to outside. The edge tilting of the slide glass is conveniently realized through the slide glass positioned in the inner region 11, and then the slide glass is taken out from the slide glass placing groove.
The slide placing turntable 1 comprises an extending branch part which extends outwards in the radial direction, and a slide placing groove is formed in the extending branch part. The dead weight of the sheet placing turntable 1 is convenient to lighten, and the rotation is convenient. Meanwhile, liquid which is convenient to centrifugally throw out is thrown out from a gap between adjacent extension branch parts.
The using method comprises the following steps:
step one, after placing the glass slide into a glass slide placing groove, driving a slide placing turntable to rotate by a driving mechanism, wherein all glass slide paths are used for spraying nozzles of pancreatin liquid in the rotating process of the slide placing turntable, and after the pancreatin liquid is sprayed on the surfaces of all glass slides, a certain treatment time is passed;
all slide ways are used for spraying physiological saline in the rotating process of the slide placing turntable, and after the physiological saline is sprayed on the surfaces of all slide ways, a certain treatment time is passed;
the method comprises the steps that in the rotating process of a slide placing turntable, all slide ways are used for spraying spray heads of dye liquor, and after the dye liquor is sprayed on the surfaces of all slide ways, a certain treatment time is passed;
the method comprises the steps that in the rotating process of a slide placing turntable, all slide ways are used for spraying spray heads of flushing liquid, and after the flushing liquid is sprayed on the surfaces of all slide ways, a certain treatment time is passed;
step two, rotating the slice placing turntable at a high speed, and spin-drying under the centrifugal action;
and step three, taking out the glass slide.
In the actual use process, the user can adjust the number of the spray heads and spray liquid of the spray heads according to the requirements.
Referring to fig. 3 to 8, embodiment 2, on the basis of embodiment 1, the drive mechanism includes a drive motor 4, and a power output shaft of the drive motor 4 is provided with a drive gear; the center of the sheet placing turntable 1 is connected with a rotating shaft 7, a driven gear is arranged on the rotating shaft 7, and a driving gear is connected with the driven gear through a driving belt; the rotating shaft 7 is rotatably arranged on a base through a bearing, and the base is arranged in a region below the processing cavity 2 in the shell 8; the driving motor is arranged on the base in a suspending way. The center of the processing cavity 2 is provided with an upward bulge part 9, and the center of the top part of the bulge part 9 is provided with an opening penetrating through a rotating shaft 7 for driving the sheet placing turntable 1 to rotate; the top of the rotating shaft 7 is detachably connected with a bracket 10, and the bottom of the center of the sheet placing turntable 1 is provided with a slot for inserting the bracket 10. The bracket and the slice placing turntable are mutually fixed through screws. The slot comprises an upper part and a lower part which are arranged up and down, and the upper part is provided with a positioning groove which extends outwards in a radial direction. The cross-sectional area of the lower part is a regular polygon.
The foregoing is merely a preferred embodiment of the present utility model and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present utility model, which are intended to be comprehended within the scope of the present utility model.
Claims (10)
1. The centrifugal dry-type chromosome banding equipment comprises a shell, wherein a processing cavity with an opening at the upper end is arranged in the shell, and is characterized in that the shell is connected with a cover plate for covering the top of the processing cavity;
the device also comprises a slice placing turntable, wherein the slice placing turntable is arranged in the processing cavity, and at least ten slide placing grooves which are circumferentially distributed are formed in the slice placing turntable;
the device also comprises a driving mechanism for driving the sheet placing turntable to rotate, wherein the driving mechanism is arranged in the shell and is in transmission connection with the sheet placing turntable;
the device comprises a processing cavity, a liquid storage bottle, a liquid supply mechanism and a spray head, wherein the liquid storage bottle is used for storing a reagent, the spray head is arranged on the inner wall of the processing cavity, the liquid storage bottle is positioned on the outer side of the processing cavity, the liquid storage bottle is connected with the spray head through a connecting pipeline, and the spray head is used for spraying the reagent on a glass slide of a glass slide placing groove;
and a liquid outlet is formed in the bottom of the treatment cavity.
2. The centrifugal dry-type chromosome banding apparatus according to claim 1, wherein: an annular groove is formed in the outer side of the bottom of the processing cavity, and the drain outlet is formed in the bottom of the annular groove.
3. The centrifugal dry-type chromosome banding apparatus according to claim 1, wherein: an auxiliary discharge outlet is formed in the side wall of the processing cavity, and the auxiliary discharge outlet is located in an area between the liquid discharge outlet and the position where the sheet placing turntable is located.
4. The centrifugal dry-type chromosome banding apparatus according to claim 1, wherein: the center of the sheet placing turntable is detachably connected with a handle.
5. The centrifugal dry-type chromosome banding apparatus according to claim 1, wherein: at least three protrusions are arranged at the bottom of the glass slide placing groove, and the heights of the protrusions are not more than 2mm.
6. The centrifugal dry-type chromosome banding apparatus according to claim 1, wherein: two corners of the radial outer side of the slide glass placing groove are respectively provided with an arc-shaped notch with a section with a central angle larger than 180 degrees, and the notch is used for accommodating the corners of the slide glass.
7. The centrifugal dry-type chromosome banding apparatus according to claim 1, wherein: the slide glass placing groove comprises an inner area and an outer area which are arranged inside and outside, wherein the radial length of the inner area is less than 1/2 of the radial length of the outer area;
the depth of the inner zone is greater than the depth of the outer zone;
the bottom of the inner zone is connected with the bottom of the outer zone through an inclined plane which is inclined upwards from inside to outside.
8. The centrifugal dry-type chromosome banding apparatus according to claim 1, wherein: the slide placing turntable comprises an extending branch part which extends outwards in the radial direction, and the slide placing groove is formed in the extending branch part.
9. The centrifugal dry-type chromosome banding apparatus according to claim 1, wherein: the driving mechanism comprises a driving motor, and a driving gear is arranged on a power output shaft of the driving motor;
the center of the sheet placing turntable is connected with a rotating shaft, a driven gear is arranged on the rotating shaft, and the driving gear is connected with the driven gear through a driving belt;
the rotating shaft is rotatably arranged on a base through a bearing, and the base is arranged in a region below the processing cavity in the shell;
the driving motor is mounted on the base in a suspending mode.
10. The centrifugal dry-type chromosome banding apparatus according to claim 9, wherein: the center of the processing cavity is provided with an upward bulge part, and the center of the top of the bulge part is provided with an opening penetrating through a rotating shaft for driving the sheet placing turntable to rotate;
the top of the rotating shaft is detachably connected with a bracket, and the bottom of the center of the sheet placing turntable is provided with a slot for inserting the bracket.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320877535.1U CN220019109U (en) | 2023-04-19 | 2023-04-19 | Centrifugal dry-type chromosome banding equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320877535.1U CN220019109U (en) | 2023-04-19 | 2023-04-19 | Centrifugal dry-type chromosome banding equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220019109U true CN220019109U (en) | 2023-11-14 |
Family
ID=88672635
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320877535.1U Active CN220019109U (en) | 2023-04-19 | 2023-04-19 | Centrifugal dry-type chromosome banding equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220019109U (en) |
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2023
- 2023-04-19 CN CN202320877535.1U patent/CN220019109U/en active Active
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