CN219935863U - Reaction device for POCT homogeneous phase luminescence immunity detection - Google Patents

Abstract

The utility model relates to a reaction device for POCT homogeneous phase luminescence immunoassay, which comprises a pre-storage cup, a reaction device and a reaction device, wherein the pre-storage cup is provided with a diluent chamber and a puncture hole thin wall which are communicated with each other, and the diluent chamber is used for containing a mixed solution of sample liquid and diluent; a reaction cup provided with a reaction chamber, wherein a drying reagent is packaged in the reaction chamber; the puncture cover is provided with a first puncture piece, and the first puncture piece is arranged in alignment with the thin wall of the puncture hole; the reaction cup is buckled in the assembling direction during use, the puncture cover is buckled in the pre-storing cup in the assembling direction, and the first puncture part punctures the puncture hole thin wall. The reaction unit for POCT homogeneous phase luminous immunodetection of this scheme only comprises prestore cup, reaction cup and three part equipment of puncture lid, simple structure, and effective manufacturing and use cost are reduced only to need in proper order along assembly direction lock with the three during the use, can accomplish mixed liquid and dry reagent contact and detect the reaction, and the simple operation is laborsaving, and the operation degree of difficulty is low, and is low to user's skill requirement.

Description

Reaction device for POCT homogeneous phase luminescence immunity detection

Technical Field

The utility model relates to the technical field of medical detection, in particular to a reaction device for POCT homogeneous phase luminescence immunoassay.

Background

POCT is an abbreviation Of Point-Of-CareTest, and is an in vitro diagnostic technique which is emerging in recent years. POCT definitions given by the national academy of clinical Biochemical sciences (NationalAcademyofClinicalBiochemistry, NACB) are: clinical laboratory tests performed by clinical personnel or patients (self-test, monitoring) not receiving clinical laboratory scientific training near patient treatment. The homogeneous photo-activated chemiluminescence technology is a fourth generation chemiluminescence technology, is a homogeneous washing-free chemiluminescence analysis technology based on nano-scale high-molecular particles, is a new generation technology with the characteristics of homogeneous reaction, no need of washing steps, high sensitivity, simplicity and convenience in operation and the like, and is a new milestone of the chemiluminescence immunoassay technology. The principle of LOCI (Luminescent oxygenchannelingimmunoassay) homogeneous luminescent diagnosis technology is as follows: intermolecular interactions will pull the donor and acceptor microspheres closer to within the singlet oxygen diffusion range, i.e., within 200nm, thereby exciting the cascade amplified chemiluminescent reaction. The luminescence principle is light excitation chemical luminescence, the surface of a donor microsphere is coated with a photosensitizer of xylylene blue, the surface of an acceptor microsphere is coated with a luminescent agent of dimethylthiophene derivative, and rare earth atom europium is chelated. Thus, when irradiated with a 680nm laser, the donor microsphere surface photosensitizer breaks down the oxygen in the environment to form monomeric oxygen molecules (oxygen radicals). The monomer oxygen molecules are diffused to the receptor microspheres, and the energy is finally transmitted to rare earth atom europium, so that the excitation light with the excitation wavelength of 615nm and the half-life period of 0.3s can be detected by adopting a chemiluminescence analyzer.

However, at present, no large-scale full-automatic equipment is available in the market for homogeneous luminescent diagnostic equipment, which is characterized by high throughput and full automation, and the equipment is not designed for POCT market, so that the equipment is large in size and high in price, and needs professional personnel to perform effective operation, so that the equipment has great application limitation.

Disclosure of Invention

The utility model aims to provide a reaction device for POCT homogeneous phase luminescence immunoassay, which aims to solve the problems that the prior art has large equipment volume, high price, inconvenient operation and use and great difficulty, and cannot be suitable for POCT homogeneous phase luminescence immunoassay.

According to an aspect of the present utility model, there is provided a reaction device for POCT homogeneous luminescent immunoassay, comprising:

the device comprises a pre-storing cup, a liquid storage device and a liquid storage device, wherein the pre-storing cup is provided with a diluent chamber and a puncture hole thin wall which are communicated, and the diluent chamber is used for containing mixed liquid of sample liquid and diluent;

the reaction cup is provided with a reaction chamber, and a dry reagent is packaged in the reaction chamber; and

the puncture cover is provided with a first puncture piece, and the first puncture piece and the puncture hole thin wall are arranged in an aligned mode; the reaction cup is buckled with the puncture cover along the assembly direction, the puncture piece punctures the thin wall of the puncture hole, and the mixed liquid of the sample liquid and the diluent in the diluent chamber flows into the reaction chamber through the punctured thin wall of the puncture hole so as to react with the dry reagent.

In one embodiment, the reaction cup is further provided with a liquid containing groove, the bottom of the liquid containing groove is provided with a containing hole, and the containing hole and the penetrating hole are arranged in a thin-wall alignment manner; the bottom of the liquid containing tank is also provided with a liquid flow channel which communicates the liquid containing tank with the reaction chamber.

In one embodiment, the reaction device for POCT homogeneous luminescent immunoassay further comprises a red blood cell filter pad mounted in the liquid receptacle and sealing the liquid inlet of the liquid flow channel.

In one embodiment, the pre-storing cup is further provided with a sealing convex edge, the shape and the size of the sealing convex edge are matched with those of the liquid containing groove, and the sealing convex edge is in sealing abutting connection with the edge of the notch of the liquid containing groove.

In one embodiment, the pre-storing cup is further provided with an air hole, and the reaction cup is further provided with an air chamber arranged in alignment with the air hole and an air chamber channel communicated with the air chamber; the diluent chamber and the air holes are simultaneously sealed and covered with a first sealing film, and the liquid containing tank and the air chamber are simultaneously sealed and covered with a second sealing film.

In one embodiment, the puncture cover is further provided with a second puncture member, the second puncture member is aligned with the air hole, and after the puncture cover, the pre-storage cup and the reaction cup are mutually buckled and assembled, the second puncture member can puncture the first sealing film and the second sealing film, so that the air hole, the air chamber and the air chamber channel are sequentially communicated.

In one embodiment, a light-transmitting window is further arranged on the side face of the reaction cup, the light-transmitting window is communicated with the reaction chamber, and a light-transmitting film is hermetically covered on the light-transmitting window.

In one embodiment, the puncture cover is further provided with a plug plunger, the plug plunger is aligned with the diluent chamber, and after the puncture cover is buckled and assembled with the pre-storing cup, the plug plunger can squeeze the mixed liquid of the sample liquid and the diluent to the thin wall of the puncture hole.

In one embodiment, the inner wall of the puncture cover is further provided with a fool-proof protruding strip, the outer wall of the pre-storing cup is further provided with a first fool-proof sliding groove, the outer wall of the reaction cup is further provided with a second fool-proof sliding groove, the second fool-proof sliding groove is in alignment communication with the first fool-proof sliding groove in the assembly direction, and the fool-proof protruding strip is slidably inserted into the first fool-proof sliding groove and the second fool-proof sliding groove; or alternatively, the process may be performed,

the outer wall of the puncture cover is provided with a first fool-proof bevel angle, the outer wall of the pre-storing cup is provided with a second fool-proof bevel angle aligned with the first fool-proof bevel angle, and the outer wall of the reaction cup is provided with a third fool-proof bevel angle aligned with the second fool-proof bevel angle.

In one embodiment, one of the pre-storing cup and the reaction cup is provided with a clamping body, the other one of the pre-storing cup and the reaction cup is provided with a clamping position, and the clamping body is in clamping connection with the clamping position; or alternatively

One of the pre-storing cup and the reaction cup is provided with a jack, the other one of the pre-storing cup and the reaction cup is provided with a plug, and the plug is fixedly connected with the jack in a plug-in manner;

the pre-storing cup and the reaction cup are of an integrated structure.

The implementation of the embodiment of the utility model has the following beneficial effects:

when the reaction device for POCT homogeneous phase luminescence immunoassay is used, firstly, sample liquid to be detected is added into a diluent chamber preloaded with diluent, so that the diluent chamber contains mixed liquid of the premixed sample liquid and the diluent; the method comprises the steps of assembling and buckling a pre-storing cup and a reaction cup, assembling and buckling a puncture cover outside the pre-storing cup, enabling a first puncture piece to directly puncture a puncture hole thin wall in the buckling process, enabling mixed liquid of sample liquid and diluent in a diluent chamber to automatically flow into a reaction chamber of the reaction cup from the punctured puncture hole thin wall, enabling the mixed liquid of the sample liquid and the diluent to react with a dry reagent, and finally transferring the whole reaction device into detection equipment, and reading a reaction signal through a sensor. Compared with the prior art, the reaction device for POCT homogeneous phase luminescence immunity detection of this scheme only comprises three parts equipment of pre-storing cup, reaction cup and puncture lid, and is simple in structure, can effectively reduce manufacturing and use cost, and only need in proper order along assembly direction lock with the three during the use, can accomplish mixed liquor and dry reagent contact and detect the reaction, the simple operation is laborsaving, and the operation degree of difficulty is low, the skill requirement to the user is low, not only professional medical personnel can be quick effectual completion detection operation, ordinary patient, ordinary nurse personnel etc. also can be high-efficient and convenient implement by oneself and detect the operation, the usability is stronger, application scope and object are wider.

Drawings

In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is an exploded schematic view of a reaction device for POCT homogeneous luminescent immunoassay according to an embodiment of the present utility model;

FIG. 2 is a schematic diagram of the assembled and buckled structure of the pre-storing cup and the reaction cup in FIG. 1;

FIG. 3 is a schematic diagram showing the assembled structure of the reaction device for POCT homogeneous luminescent immunoassay in FIG. 1;

FIG. 4 is a top view block diagram of the pre-reservoir;

FIG. 5 is an isometric view of a pre-store cup;

FIG. 6 is a bottom view of the pre-cup;

FIG. 7 is a top view of the block diagram of FIG. 6;

FIG. 8 is an isometric view of a reaction cup;

FIG. 9 is a top view of the block diagram of FIG. 8;

FIG. 10 is a bottom view of the structure of FIG. 9;

FIG. 11 is an isometric view of the lancing cap;

FIG. 12 is a bottom view of the structure of FIG. 11;

FIG. 13 is a top view of the block diagram of FIG. 12;

FIG. 14 is an exploded schematic view of another embodiment of a reaction device for POCT homogeneous luminescent immunoassay;

FIG. 15 is a schematic view of the assembled snap-fit of the pre-cup and the reaction cup of FIG. 14;

FIG. 16 is a schematic diagram showing the assembled structure of the reaction device for POCT homogeneous luminescent immunoassay in FIG. 14;

FIG. 17 is an exploded schematic view of a reaction device for POCT homogeneous luminescent immunoassay in a third embodiment;

FIG. 18 is an assembly block diagram of FIG. 17;

FIG. 19 is a schematic view of the structure of the puncture cap;

FIG. 20 is a bottom view of the lancing cap of FIG. 19;

FIG. 21 is a schematic view of the structure of a pre-storing cup and a reaction cup of an integrated structure;

FIG. 22 is a top view of the block diagram of FIG. 21;

fig. 23 is a front view of the structure of fig. 21.

Wherein:

100. the reaction device is used for POCT homogeneous phase luminescence immunoassay;

10. pre-storing a cup; 11. a diluent chamber; 12. puncturing the thin wall of the hole; 13. sealing the convex edge; 14. ventilation holes; 15. a first fool-proof chute; 16. a second fool-proof bevel; 17. a card body; 18. inserting a column;

20. a reaction cup; 21. a reaction chamber; 22. a liquid container; 221. an accommodation hole; 222. a flow channel; 23. a red blood cell filter pad; 24. a gas chamber; 25. a plenum channel; 26. a light-transmitting window; 27. a light-transmitting film; 28. a second fool-proof chute; 29. a third fool-proof bevel; 29a, clamping position; 29b, a jack;

30. a puncture cap; 31. a first piercing member; 32. a second piercing member; 33. a corking post; 34. fool-proof raised strips; 35. a first fool-proof bevel.

Detailed Description

In order that the utility model may be readily understood, a more complete description of the utility model will be rendered by reference to the appended drawings. Preferred embodiments of the present utility model are shown in the drawings. This utility model may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

It will be understood that when an element is referred to as being "fixed to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this utility model belongs. The terminology used herein in the description of the utility model is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.

Referring to fig. 1, for a reaction apparatus 100 for POCT homogeneous luminescent immunoassay according to an embodiment of the present utility model, the principle of POCT homogeneous luminescent immunoassay can be summarized as follows: under homogeneous condition, the sample to be tested is mixed with luminescent microsphere (nanometer level) containing luminescent material, photosensitive microsphere (nanometer level) containing photosensitive material and other reagents, and the bioactive molecule (antibody, antigen and other) is connected to the surface of the luminescent microsphere and the photosensitive microsphere to capture the target molecule in the sample to be tested directly or indirectly, so as to form the immune sandwich compound of the luminescent microsphere, the target molecule and the photosensitive microsphere. After the excitation light is irradiated, the photosensitive microsphere is induced to activate and release active oxygen molecules in a high energy state. The high-energy active oxygen molecules are captured by the luminescent microspheres at close range, thereby transferring energy to activate the luminescent compounds in the luminescent microspheres. After a few microseconds, the luminescent compound in the luminescent microsphere will release high-level red light, these high-level photons are measured with a single photon counter, and the photon number is converted into the target molecule concentration by a computer. When the sample does not contain target molecules, an immune sandwich compound cannot be formed, active oxygen ions are released from the photosensitive microspheres under the irradiation of excitation light, but cannot reach the luminous microspheres, namely, the sample is rapidly quenched in a liquid phase, and no high-energy-level red light is generated during detection.

Referring to fig. 1 to 16, a reaction device 100 for POCT homogeneous luminescent immunoassay includes a pre-cup 10, a reaction cup 20, and a puncture cap 30, as an example. The pre-storing cup 10 is provided with a diluent chamber 11 and a puncture thin wall 12 which are communicated, and the diluent chamber 11 is used for containing a mixed liquid of sample liquid and diluent. The sample liquid may be any one of serum, plasma, whole blood, urine, saliva, and the like.

The reaction cup 20 is provided with a reaction chamber 21, and a dry reagent is encapsulated in the reaction chamber 21. Wherein, the dry reagent can be photosensitive microsphere freeze-dried beads and luminous microsphere freeze-dried beads.

The puncture cap 30 is provided with a first puncture member 31, and the first puncture member 31 is arranged in alignment with the puncture hole thin wall 12; when in use, the pre-storage cup 10 is buckled on the reaction cup 20 along the assembling direction, the puncture cover 30 is buckled on the pre-storage cup 10 along the assembling direction, the first puncture member 31 punctures the puncture hole thin wall 12, and the mixed liquid of the sample liquid and the diluent in the diluent chamber 11 flows into the reaction chamber 21 through the punctured puncture hole thin wall 12 so as to react with the dry reagent.

It will be appreciated that the above-described assembly direction refers specifically to the direction of the arrow in the drawings.

The implementation of the embodiment of the utility model has the following beneficial effects:

when the reaction device 100 for POCT homogeneous phase luminescence immunoassay of the above-mentioned scheme is used, firstly, a sample liquid to be tested is added into a diluent chamber 11 preloaded with a diluent, so that the diluent chamber 11 contains a mixed solution of the premixed sample liquid and the diluent; the pre-storing cup 10 and the reaction cup 20 are assembled and buckled, the puncture cover 30 is assembled and buckled outside the pre-storing cup 10, the first puncture piece 31 can directly puncture the puncture hole thin wall 12 in the buckling process, so that the mixed liquid of the sample liquid and the diluent in the diluent chamber 11 can automatically flow into the reaction chamber 21 of the reaction cup 20 from the punctured puncture hole thin wall 12, the mixed liquid of the sample liquid and the diluent can react with a dry reagent, and finally the whole reaction device is transferred into the detection equipment to read a reaction signal through a sensor.

Compared with the prior art, the reaction device 100 for POCT homogeneous phase luminescence immunoassay of this scheme only comprises three parts assembly of pre-storing cup 10, reaction cup 20 and puncture lid 30, and is simple in structure, the whole tiny light of device volume, can effectively reduce manufacturing and use cost, and only need when using along assembly direction lock in proper order with the three, can accomplish mixed liquid and dry reagent contact and detect the reaction, the simple operation is laborsaving, and the operation degree of difficulty is low, the skill requirement to the user is low, not only professional medical personnel can quick effectual completion detect operation, ordinary patient, ordinary nurse personnel etc. also can high-efficient and convenient self-implementation detect operation, the usability is stronger, application scope and object are wider.

In the utility model, the puncture cover 30, the pre-storing cup 10 and the reaction cup 20 all adopt rectangular cube-shaped appearance structures, and have regular multiple side surfaces, so that the puncture cover is convenient for a user to effectively grasp and mutually buckle and assemble.

It will be appreciated that the above-mentioned assembling direction refers to a direction in which the puncture cap 30, the pre-storing cup 10 and the reaction cup 20 are sequentially stacked or nested along the height direction. For example, in the present embodiment, the pre-cup 10 and the reaction cup 20 are stacked and assembled only in the assembly direction, and the puncture cap 30 is completely nested outside the pre-cup 10 and a part of the reaction cup 20 to form an inner and outer double-layered structure.

To reduce manufacturing and use costs, the piercing cap 30, the pre-reservoir 10 and the reaction cup 20 are preferably all made of medical grade plastic materials. Of course, other embodiments may employ other types of piercing cap 30, pre-cup 10, and reaction cup 20, such as metals, composites, etc.

Referring to fig. 4 to 7, in one embodiment, the reaction cup 20 is further provided with a liquid containing groove 22, and the liquid containing groove 22 is specifically formed on the top surface of the reaction cup 20 facing the pre-storing cup 10. The bottom of the liquid containing groove 22 and at one vertex angle of the reaction cup 20 are provided with a containing hole 221, and the containing hole 221 is aligned with the penetration hole thin wall 12. After the puncture cap 30 is buckled and assembled with the pre-storing cup 10 and the reaction cup 20, the first puncture member 31 punctures the puncture hole thin wall 12 and then can extend into the accommodating hole 221, so that the accommodating hole 221 accommodates the first puncture member 31, and interference between the reaction cup 20 and the first puncture member 31 is avoided.

The bottom of the liquid containing groove 22 is also provided with a liquid flow channel 222, and the liquid flow channel 222 communicates the liquid containing groove 22 with the reaction chamber 21. When the thin wall 12 of the puncture hole is punctured, the mixed solution of the sample liquid and the diluent flows into the liquid containing groove 22 through the thin wall 12 of the puncture hole, then slowly flows into the liquid flow channel 222, and finally flows into the reaction chamber 21 below from the liquid flow channel 222, so that the mixed solution of the sample liquid and the diluent gradually and slowly flows into the reaction chamber 21 to be in full contact reaction with the dry reagent.

Further, the reaction device 100 for POCT homogeneous luminescent immunoassay further comprises a red blood cell filter pad 23, wherein the red blood cell filter pad 23 is disposed in the liquid container 22 and covers the liquid inlet of the liquid flow channel 222.

Whether chemiluminescent immunoassay or photoexcitation immunoassay, the final detection is a reaction-generated optical signal. Meanwhile, the biggest scene of the application of immunodetection is to detect various objects to be detected in blood. Since a large number of red blood cells exist in blood, the red blood cells and heme in the red blood cells have strong light absorption characteristics and greatly interfere with optical signals generated by the reaction. If red blood cells exist in the detection reaction system, the accuracy of the detection result can be greatly influenced. Compared with the prior art that an additional red blood cell separation device or apparatus (such as a centrifuge) is required to perform an additional red blood cell separation operation, which results in time and cost increase, the method has the advantages that the red blood cell filter pad 23 is pre-installed in the liquid containing tank 22, the mixed liquid of the sample liquid and the diluent flowing into the liquid containing tank 22 can flow through the red blood cell filter pad 23 first, so that the red blood cells can be automatically separated, the red blood cells are prevented from flowing into the reaction chamber 21 to interfere with the accuracy of the detection result, and meanwhile, the additional red blood cell separation device or apparatus is not required to be additionally provided, so that the reaction apparatus has the advantages of lower cost, simpler structure and shorter detection time.

In particular, the red blood cell filter pad 23 may be laminated with two or more layers at the same time, and the mixed solution sequentially flows through each layer of red blood cell filter pad 23 to be filtered at least twice, thereby more thoroughly filtering red blood cells.

In another embodiment, the pre-storing cup 10 is further provided with a sealing convex edge 13, the shape and the size of the sealing convex edge 13 are matched with those of the liquid containing groove 22, and the sealing convex edge 13 is in sealing abutting connection with the edge of the notch of the liquid containing groove 22. Through the sealed butt of sealed protruding edge 13 and the notch border of flourishing cistern 22, can realize that the pre-storing cup 10 and reaction cup 20 lock equipment after realize the encapsulation to flourishing cistern 22, prevent that the mixed liquid of sample liquid and diluent from taking place to leak.

Further, a sealing ring (such as a rubber ring) can be optionally installed between the sealing flange 13 and the edge of the notch of the liquid containing tank 22 to enhance the sealing performance.

In addition, in yet another embodiment, the pre-storing cup 10 is further provided with a ventilation hole 14, the reaction cup 20 is further provided with a gas chamber 24 arranged in alignment with the ventilation hole 14, and a gas chamber channel 25 communicated with the gas chamber 24; the diluent chamber 11 and the ventilation holes 14 are simultaneously sealed and covered with a first sealing film, and the liquid containing tank 22 and the air chamber 24 are simultaneously sealed and covered with a second sealing film. That is, when the reaction device (the reaction device 100 for POCT homogeneous luminescent immunoassay is abbreviated as "the following description" for short "), the sample liquid and the diluent in the diluent chamber 11 can be temporarily packaged in the diluent chamber 11, so as to avoid spilling and being affected by the temperature and humidity of the external environment; and the dry reagent can be enclosed in the reaction chamber 21 to prevent the dry reagent from being exposed to the outside air and becoming wet and ineffective.

Alternatively, the first sealing film and the second sealing film may be aluminum films or plastic films. The packaging can be processed by adopting a heat sealing process.

Referring to fig. 4 to 13, further, the puncture cap 30 is further provided with a second puncture member 32, the second puncture member 32 is aligned with the air hole 14, and after the puncture cap 30, the pre-storage cup 10 and the reaction cup 20 are assembled by fastening, the second puncture member 32 punctures the first sealing film and the second sealing film, so that the air hole 14, the air chamber 24 and the air chamber channel 25 are sequentially communicated.

That is, after the puncture cap 30, the pre-storage cup 10 and the reaction cup 20 are assembled and buckled, the first puncture member 31 punctures the puncture hole thin wall 12, when the mixed liquid of the sample liquid and the diluent flows to the reaction chamber 21, the second puncture member 32 punctures the corresponding parts of the first sealing film and the second sealing film packaged on the air hole 14 and the air chamber 24 simultaneously, so that the air hole 14, the air chamber 24 and the air chamber channel 25 can be communicated, the inside of the reaction device is communicated with the external environment to form balanced air pressure, and the mixed liquid can flow smoothly to the reaction chamber 21.

Optionally, in this scheme, the first piercing member 31 and the second piercing member 32 are both the rod piece protruding on the inside of the piercing cap 30, the end of the rod piece is formed into a pointed cone structure, when the piercing cap 30, the pre-storing cup 10 and the reaction cup 20 are pressed by the user, the pointed cone structure can puncture the piercing hole thin wall 12, the first sealing film and the second sealing film fast and reliably, so that the operation difficulty of the reaction device is low, and the reaction device is convenient and labor-saving, and the patient and family member of the patient without the operation skill and experience of medical equipment can also use easily.

In one embodiment, the side surface of the reaction cup 20 is further provided with a light-transmitting window 26, the light-transmitting window 26 is communicated with the reaction chamber 21, and a light-transmitting film 27 is hermetically covered on the light-transmitting window 26.

After the dry reagent is filled into the reaction chamber 21, the light-transmitting film 27 is installed at the light-transmitting window 26 in a heat sealing way, so that the dry reagent can be completely sealed outside the sealed reaction chamber 21, the light-transmitting film 27 has good permeability to light with the wavelength of about 680nm and about 615nm as the light-transmitting film is not blocked by the puncture cover 30, and excitation light and emission light can be transmitted during homogeneous luminescent immunoassay, so that the detection process is smoothly carried out.

For example, the light-transmitting film 27 may be a transparent plastic film.

Referring to fig. 12 to 13, in addition, in another embodiment, the puncture cap 30 is further provided with a plunger 33, the plunger 33 is aligned with the diluent chamber 11, and after the puncture cap 30 is assembled with the pre-storing cup 10, the plunger 33 can squeeze the mixture of the sample liquid and the diluent toward the puncture thin wall 12. Specifically, the plunger 33 has a shape matching with the shape of the diluent chamber 11, when the puncture cap 30 is assembled and fastened to the outside of the pre-reservoir 10, the plunger 33 can perfectly fit with the diluent chamber 11, thereby forming a piston action in the diluent chamber 11, and as the puncture cap 30 is continuously fastened to the pre-reservoir 10, the pressure of the plunger 33 on the diluent chamber 11 gradually increases, and since the aperture formed after the puncture of the puncture thin wall 12 by the first puncture member 31 is generally smaller, the mixture of the pressurized sample liquid and the diluent flows first toward the puncture thin wall 12, so as to more easily pass through the aperture of the puncture thin wall 12 and flow into the liquid container 22, and under the condition of continuously increasing pressure, the mixture in the liquid container 22 can continuously pass through the red blood cell filter pad 23, and finally flow into the reaction chamber 21 through the liquid flow.

In order to avoid that the puncture cover 30, the pre-storing cup 10 and the reaction cup 20 are assembled in the wrong direction and cannot be successfully and correctly buckled, in one embodiment, the puncture cover is characterized in that the inner wall of the puncture cover 30 is also provided with a fool-proof raised line 34, the outer wall of the pre-storing cup 10 is also provided with a first fool-proof sliding chute 15, the outer wall of the reaction cup 20 is also provided with a second fool-proof sliding chute 28, the second fool-proof sliding chute 28 is in counterpoint communication with the first fool-proof sliding chute 15 in the assembling direction, and the fool-proof raised line 34 is slidably inserted into the first fool-proof sliding chute 15 and the second fool-proof sliding chute 28; or, the outer wall of the puncture cap 30 is provided with a first fool-proof bevel 35, the outer wall of the pre-storing cup 10 is provided with a second fool-proof bevel 16 aligned with the first fool-proof bevel 35, and the outer wall of the reaction cup 20 is provided with a third fool-proof bevel 29 aligned with the second fool-proof bevel 16.

That is, in a simple manner, only if the fool-proof protruding strips 34 are aligned with the first fool-proof sliding grooves 15 and the second fool-proof sliding grooves 28 at the same time or the first fool-proof oblique angles 35, the second fool-proof oblique angles 16 and the third fool-proof oblique angles 29 are aligned on the same straight line, the puncture cap 30, the pre-stored cup 10 and the reaction cup 20 can be assembled into a whole correctly.

Further, one of the pre-storing cup 10 and the reaction cup 20 is provided with a clamping body 17, the other one of the pre-storing cup 10 and the reaction cup 20 is provided with a clamping position 29a, and the clamping body 17 is in clamping connection with the clamping position 29 a; alternatively, one of the pre-cup 10 and the reaction cup 20 is provided with a jack 29b, the other of the pre-cup 10 and the reaction cup 20 is provided with a plug 18, and the plug 18 is fixedly inserted into the jack 29 b. Therefore, by means of the assembly mode that the clamping body 17 is in buckling connection with the clamping position 29a or the inserting column 18 is inserted into the inserting hole 29b, the pre-storing cup 10 and the reaction cup 20 can be connected and fixed, a liquid flow passage and an air flow passage with good sealing are formed between the pre-storing cup 10 and the reaction cup 20, and the mixed liquid can safely and reliably flow into the reaction chamber 21 to be in effective contact with the dry reagent to finish detection.

Referring to fig. 17 to 23, a reaction apparatus of a third embodiment is provided, which is different from the two embodiments described above in that the reaction cup 20 and the pre-cup 10 are integrated, that is, the reaction apparatus is only provided with two parts, the sealing film is only required to be two, one piece is used for sealing the pre-cup and the vent hole of the diluent at the top, and one piece covers the red blood cell separation area and the reaction chamber at the same time, so that the number of parts can be further reduced, and the production procedures of the production process of the product can be reduced, thereby making the production of the kit more efficient and lower in cost. The working principle is basically the same as that of the above embodiment, and thus, further description is not given here.

The above examples illustrate only a few embodiments of the utility model, which are described in detail and are not to be construed as limiting the scope of the claims. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the utility model, which are all within the scope of the utility model. Accordingly, the scope of protection of the present utility model is to be determined by the appended claims.

Claims (10)

1. A reaction device for POCT homogeneous luminescent immunoassay, comprising:

the device comprises a pre-storing cup, a liquid storage device and a liquid storage device, wherein the pre-storing cup is provided with a diluent chamber and a puncture hole thin wall which are communicated, and the diluent chamber is used for containing mixed liquid of sample liquid and diluent;

the reaction cup is provided with a reaction chamber, and a dry reagent is packaged in the reaction chamber; and

the puncture cover is provided with a first puncture piece, and the first puncture piece and the puncture hole thin wall are arranged in an aligned mode; the reaction cup is buckled with the puncture cover along the assembly direction, the puncture piece punctures the thin wall of the puncture hole, and the mixed liquid of the sample liquid and the diluent in the diluent chamber flows into the reaction chamber through the punctured thin wall of the puncture hole so as to react with the dry reagent.

2. The reaction device for POCT homogeneous phase luminescence immunoassay according to claim 1, wherein the reaction cup is further provided with a liquid containing groove, the bottom of the liquid containing groove is provided with a containing hole, and the containing hole and the penetrating hole are arranged in thin wall alignment; the bottom of the liquid containing tank is also provided with a liquid flow channel which communicates the liquid containing tank with the reaction chamber.

3. The reaction device for POCT homogeneous luminescent immunoassay of claim 2, further comprising a red blood cell filter pad disposed within the reservoir and covering the liquid inlet of the liquid flow channel.

4. The reaction device for POCT homogeneous luminescent immunodetection of claim 2, wherein the pre-reservoir is further provided with a sealing flange, the sealing flange being adapted to the shape and size of the liquid container, the sealing flange being in sealing abutment with the notch edge of the liquid container.

5. The reaction device for POCT homogeneous phase luminescence immunoassay according to claim 2, wherein the pre-storing cup is further provided with an air vent, the reaction cup is further provided with an air chamber arranged in alignment with the air vent, and an air chamber channel communicated with the air chamber; the diluent chamber and the air holes are simultaneously sealed and covered with a first sealing film, and the liquid containing tank and the air chamber are simultaneously sealed and covered with a second sealing film.

6. The reaction device for POCT homogeneous phase luminescence immunoassay according to claim 5, wherein the puncture cover is further provided with a second puncture member, the second puncture member is aligned with the air hole, and after the puncture cover, the pre-storing cup and the reaction cup are assembled by being buckled with each other, the second puncture member can puncture the first sealing film and the second sealing film, so that the air hole, the air chamber and the air chamber channel are sequentially communicated.

7. The reaction device for POCT homogeneous phase luminescence immunoassay according to claim 1, wherein a light-transmitting window is further arranged on the side face of the reaction cup, the light-transmitting window is communicated with the reaction chamber, and a light-transmitting film is hermetically covered on the light-transmitting window.

8. The reaction device for POCT homogeneous luminescent immunodetection of claim 1, wherein the puncture cap is further provided with a plunger, the plunger is aligned with the diluent chamber, and the plunger can squeeze the mixture of the sample liquid and the diluent to the puncture thin wall after the puncture cap is assembled with the pre-cup.

9. The reaction device for POCT homogeneous luminescent immunity detection according to any one of claims 1 to 8, wherein the inner wall of the puncture cap is further provided with a fool-proof protruding strip, the outer wall of the pre-storing cup is further provided with a first fool-proof chute, the outer wall of the reaction cup is further provided with a second fool-proof chute, the second fool-proof chute is in alignment communication with the first fool-proof chute in the assembly direction, and the fool-proof protruding strip is slidably inserted into the first fool-proof chute and the second fool-proof chute; or alternatively, the process may be performed,

the outer wall of the puncture cover is provided with a first fool-proof bevel angle, the outer wall of the pre-storing cup is provided with a second fool-proof bevel angle aligned with the first fool-proof bevel angle, and the outer wall of the reaction cup is provided with a third fool-proof bevel angle aligned with the second fool-proof bevel angle.

10. The reaction device for POCT homogeneous luminescent immunodetection according to any one of claims 1 to 8, wherein one of the pre-reservoir and the reaction cup is provided with a clamp body, the other of the pre-reservoir and the reaction cup is provided with a clamp, and the clamp body is in snap connection with the clamp; or alternatively

One of the pre-storing cup and the reaction cup is provided with a jack, the other one of the pre-storing cup and the reaction cup is provided with a plug, and the plug is fixedly connected with the jack in a plug-in manner;

the pre-storing cup and the reaction cup are of an integrated structure.