CN219935684U - Mycotoxin detection test paper - Google Patents
Mycotoxin detection test paper Download PDFInfo
- Publication number
- CN219935684U CN219935684U CN202223490872.XU CN202223490872U CN219935684U CN 219935684 U CN219935684 U CN 219935684U CN 202223490872 U CN202223490872 U CN 202223490872U CN 219935684 U CN219935684 U CN 219935684U
- Authority
- CN
- China
- Prior art keywords
- strip
- bottom plate
- test
- shaped groove
- mycotoxin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000012360 testing method Methods 0.000 title claims abstract description 72
- 231100000678 Mycotoxin Toxicity 0.000 title claims abstract description 30
- 239000002636 mycotoxin Substances 0.000 title claims abstract description 30
- 238000001514 detection method Methods 0.000 title claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 108091023037 Aptamer Proteins 0.000 claims description 7
- 239000002245 particle Substances 0.000 claims description 7
- 238000011161 development Methods 0.000 claims description 3
- 238000005265 energy consumption Methods 0.000 abstract description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 6
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 4
- 239000010931 gold Substances 0.000 description 4
- 229910052737 gold Inorganic materials 0.000 description 4
- 108091008104 nucleic acid aptamers Proteins 0.000 description 4
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 3
- 239000007822 coupling agent Substances 0.000 description 3
- 239000013078 crystal Substances 0.000 description 3
- 229960000304 folic acid Drugs 0.000 description 3
- 235000019152 folic acid Nutrition 0.000 description 3
- 239000011724 folic acid Substances 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 239000010453 quartz Substances 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 108090000190 Thrombin Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 229930183344 ochratoxin Natural products 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- TXDNPSYEJHXKMK-UHFFFAOYSA-N sulfanylsilane Chemical compound S[SiH3] TXDNPSYEJHXKMK-UHFFFAOYSA-N 0.000 description 2
- 229960004072 thrombin Drugs 0.000 description 2
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 2
- 229940038773 trisodium citrate Drugs 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001294 liquid chromatography-tandem mass spectrometry Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The utility model provides mycotoxin detection test paper, which comprises a bottom plate, wherein one side of the upper surface of the bottom plate is recessed downwards to form a strip-shaped groove for storing sample liquid, a plurality of test strips for detecting mycotoxin are uniformly arranged on the upper surface of the bottom plate, the test strips are vertically arranged with the strip-shaped groove, one end of each test strip extends to the upper side of the corresponding strip-shaped groove, the part of each test strip, which is positioned at the corner of the corresponding strip-shaped groove, is hinged with the bottom plate, one end of each test strip, which is far away from the corresponding strip-shaped groove, is fixed with a support strip, the part, which is connected with the corresponding test strip, is provided with a crease, one side, which is far away from the corresponding strip-shaped groove, of the bottom plate is recessed downwards to form a positioning groove for limiting the end position of the corresponding support strip, and the positioning groove is arranged in parallel with the corresponding strip-shaped groove, and the mycotoxin detection test paper has the following advantages: the depth of the mycotoxin detection test paper extending into the sample liquid is not required to be controlled by personnel, so that the energy consumption of the personnel is reduced.
Description
Technical Field
The utility model relates to mycotoxin detection test paper, and belongs to the field of detection test paper.
Background
The detection of the mycotoxins mainly comprises chromatography, mass spectrometry, LC-MS/MS and the like, and the methods have the defects of complicated and tedious sample pretreatment, long time consumption, inapplicability to field operation and the like, so the mycotoxin detection test paper is a common detection consumable, currently, when the same sample liquid is detected by the mycotoxin detection test paper, the same sample liquid is generally required to be detected by a plurality of mycotoxin detection test papers, then the plurality of mycotoxin detection test papers are compared, one end of the mycotoxin detection test paper needs to be immersed into the sample liquid one by one in the operation process, and the depth of the end of the mycotoxin detection test paper extending into the sample liquid is controlled by people, so that the labor of people is increased.
Disclosure of Invention
Aiming at the defects existing in the prior art, the utility model aims to provide the mycotoxin detection test paper so as to solve the problems in the background art.
In order to achieve the above object, the present utility model is realized by the following technical scheme: the utility model provides a mycotoxin test paper, includes the bottom plate, bottom plate upper surface one side undercut forms the bar groove that is used for storing sample liquid, the bottom plate upper surface evenly is equipped with a plurality of test strips that are used for detecting mycotoxin, the test strip is arranged perpendicularly with the bar groove, test strip one end extends to bar groove upside, the test strip is in the position and the bottom plate hinge connection of bar groove edges and corners department, the one end that the bar groove was kept away from to the test strip is fixed with the support bar, the support bar has the crease with the processing of the position that the test strip is connected, one side undercut that the bar groove was kept away from to the bottom plate upper surface forms the constant head tank that is used for restricting support bar tip position, constant head tank and bar groove parallel arrangement.
Further, the test strip comprises a base strip, a suction strip is fixed on the upper surface of the base strip, a detection strip is fixed on the upper surface of the suction strip, a color development film is coated on the upper surface of the detection strip at a position close to a strip groove, agglomerate particles are uniformly coated on the surface of the suction strip, the color development film is prepared from 13 parts by mass of nano gold particles, 0.7 part of thrombin antibody, 1 part of quartz crystal, 8 parts of mercaptosilane coupling agent and 8 parts of folic acid, an aptamer layer is uniformly coated on the surface of the detection strip, the aptamer layer consists of 37 parts by mass of nucleic acid aptamer, 22 parts by mass of chloroauric acid, 27 parts by mass of trisodium citrate and 16 parts by mass of Ding Shaan alcohol, and the sequence of the nucleic acid aptamer is 5'-GATCGGGTGTGGGTGGCGTAAAGGGAGCATCGGACAAGAAATGCCACA-3'.
Further, the upper surface of the bottom plate is recessed downwards to form a rectangular opening, and the rectangular opening is arranged between the strip-shaped groove and the positioning groove.
Further, the bottom plate is of a rectangular structure, and round corners are machined at four edges of the bottom plate.
Further, four corners of the upper surface of the bottom plate are fixedly connected with rubber heads, the cross section of each rubber head is round, positioning holes matched with the rubber heads are formed in the four corners of the lower surface of the bottom plate, and the inner diameter of each positioning hole is the same as the outer diameter of each rubber head.
Further, a blind hole is formed in the position, where the rubber head is mounted, of the bottom plate, and one end of the rubber head is stuck in the blind hole.
Further, a film is fixed at the edge of the upper part of one side surface in the strip-shaped groove, and the other end of the film is fixedly connected with the test strip.
The utility model has the beneficial effects that:
1. the test strips are arranged on the bottom plate in parallel, the test strips are hinged at the strip-shaped groove by the film, the sample liquid is added into the strip-shaped groove, the support strips are bent along the crease, one ends of the support strips are inserted into the positioning groove, at the moment, the support strips and the test strips are in a certain angle, under the support of the support strips, the test strips are in an inclined state without personnel to keep the test strips, and the support strips are in a certain size, so that the depth of the ends of the test strips extending into the sample liquid is certain under the support of the support strips, the depth of the test strips extending into the sample liquid is not required to be controlled manually, and the energy consumption of personnel is reduced.
2. Four rubber heads on one bottom plate are respectively inserted into positioning holes on the other bottom plate, so that the insertion of two adjacent bottom plates is realized, and the like, the insertion of a plurality of bottom plates is finished, and the plurality of bottom plates are conveniently placed in order.
Drawings
Other features, objects and advantages of the present utility model will become more apparent upon reading of the detailed description of non-limiting embodiments, given with reference to the accompanying drawings in which:
FIG. 1 is a schematic diagram of the structure of a mycotoxin test paper of the present utility model;
FIG. 2 is an enlarged view of FIG. 1 at A;
FIG. 3 is a perspective view of a bottom plate of a test strip for detecting mycotoxins in accordance with the present utility model;
FIG. 4 is a cross-sectional view of a test strip of the mycotoxin test strip of the present utility model;
in the figure: 1-bottom plate, 2-display film, 3-detection strip, 4-bar slot, 5-rectangular mouth, 6-rubber head, 7-support bar, 8-crease, 9-positioning slot, 10-round angle, 11-film, 12-positioning hole, 13-base strip, 14-suction strip.
Detailed Description
The utility model is further described in connection with the following detailed description, in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the utility model easy to understand.
Referring to fig. 1 and 2, the present utility model provides a technical solution: the utility model provides a mycotoxin test paper, including bottom plate 1, bottom plate 1 upper surface one side undercut forms the bar groove 4 that is used for storing the sample liquid, evenly arrange a plurality of test strips that are used for detecting mycotoxin at bottom plate 1 upper surface, wherein test strip and bar groove 4 arrange perpendicularly and test strip one end extends to bar groove 4 upside, make fixed connection's film 11 other end fix in bar groove 4 medial surface upper portion edge on test strip, the restriction to test strip position is realized to film 11 this moment, the test strip forms hinge connection relation with bottom plate 1 promptly, be convenient for make the test strip present incline state.
Referring to fig. 1 and 4, the test strip comprises a base strip 13, a suction strip 14 is fixed on the upper surface of the base strip 13, a detection strip 3 is fixed on the upper surface of the suction strip 14, a color developing film is coated on the upper surface of the detection strip 3 at a position close to a strip groove 4, wherein agglomerated particles are uniformly coated on the surface of the suction strip 14, the color developing film is prepared from 13 parts by mass of nano gold particles, 0.7 part of thrombin antibody, 1 part of quartz crystal, 8 parts of mercaptosilane coupling agent and 8 parts of folic acid, an aptamer layer is uniformly coated on the surface of the detection strip 3, wherein the aptamer layer is composed of 37 parts by mass of nucleic acid aptamer, 22 parts of chloroauric acid, 27 parts of trisodium citrate and 16 parts of Ding Shaan alcohol, the sequence of the nucleic acid aptamer is 5'-GATCGGGTGTGGGTGGCGTAAAGGGAGCATCGGACAAGAAATGCCACA-3', the color developing film is red in a normal state, when ochratoxin is detected, the color developing effect of the nano gold particles can be enhanced by crosslinking the folic acid, the quartz crystal and the nano gold particles can be amplified by the coupling agent, and finally, the rapid sensitive detection of the ochratoxin can be realized by carrying out aptamer screening on the aptamer by the detection strip 3.
Referring to fig. 1, 2 and 3, a supporting bar 7 is fixed at one end of the test strip far away from the strip groove 4, wherein a crease 8 is processed at the position where the supporting bar 7 is connected with the test strip, one side of the upper surface of the bottom plate 1 far away from the strip groove 4 is downwards sunken to form a positioning groove 9 which is used for limiting the position of the end of the supporting bar 7 and is arranged in parallel with the strip groove 4, a plurality of test strips are arranged on the bottom plate 1 in parallel, the test strip is hinged at the strip groove 4 by utilizing a film 11, after sample liquid is added into the strip groove 4, the supporting bar 7 is bent along the crease 8, one end of the supporting bar 7 is inserted into the positioning groove 9, at the moment, the supporting bar 7 and the test strip are in a certain angle, under the support of the supporting bar 7, personnel does not need to keep the state of the test strip, therefore, the depth of the end of the test strip is certain under the support of the supporting bar 7, the personnel does not need to control the depth of the test strip extending into the sample liquid, the consumption of the personnel is reduced, the material consumption of the personnel is reduced, the sharp corner is reduced at the position between the rectangular notch 4 and the rectangular notch 1, and the corner 10 is reduced at the corner of the bottom plate 1.
Referring to fig. 1, 2 and 3, rubber heads 6 with circular cross sections are stuck in blind holes formed in four corners of the upper surface of a base plate 1, the range of connection between the rubber heads 6 and the base plate 1 is enlarged by the blind holes, then positioning holes 12 matched with the rubber heads 6 are formed in four corners of the lower surface of the base plate 1, wherein the inner diameters of the positioning holes 12 are the same as the outer diameters of the rubber heads 6, so that the four rubber heads 6 on one base plate 1 are respectively inserted into the positioning holes 12 on the other base plate 1, the adjacent two base plates 1 are inserted, and the like, the insertion of a plurality of base plates 1 is completed, and the plurality of base plates 1 are placed neatly.
Although the present disclosure describes embodiments, not every embodiment is described in terms of a single embodiment, and such description is for clarity only, and one skilled in the art will recognize that the embodiments described in the disclosure as a whole may be combined appropriately to form other embodiments that will be apparent to those skilled in the art.
Claims (7)
1. Mycotoxin test paper, including bottom plate (1), its characterized in that: the test strip comprises a bottom plate (1), wherein a strip-shaped groove (4) for storing sample liquid is formed in one side of the upper surface of the bottom plate (1) in a downward sinking mode, a plurality of test strips for detecting mycotoxins are uniformly arranged on the upper surface of the bottom plate (1), the test strips are vertically arranged with the strip-shaped groove (4), one end of each test strip extends to the upper side of the corresponding strip-shaped groove (4), the part, located at the corner of the strip-shaped groove (4), of each test strip is hinged with the bottom plate (1), a support strip (7) is fixed at one end, away from the strip-shaped groove (4), of each test strip (7), a crease (8) is formed in the connected part of each support strip (7), and a positioning groove (9) for limiting the end position of each support strip (7) is formed in a downward sinking mode on one side, away from the strip-shaped groove (4), and the positioning groove (9) is arranged in parallel with the strip-shaped groove (4).
2. The mycotoxin test paper of claim 1, wherein: the test strip comprises a base strip (13), a suction strip (14) is fixed on the upper surface of the base strip (13), a detection strip (3) is fixed on the upper surface of the suction strip (14), a color development film is covered on the upper surface of the detection strip (3) at a position close to the strip groove (4), aggregate particles are uniformly coated on the surface of the suction strip (14), and an aptamer layer is uniformly coated on the surface of the detection strip (3).
3. The mycotoxin test paper of claim 1, wherein: the upper surface of the bottom plate (1) is recessed downwards to form a rectangular opening (5), and the rectangular opening (5) is arranged between the strip-shaped groove (4) and the positioning groove (9).
4. A test strip for mycotoxin detection as defined in claim 3, wherein: the base plate (1) is of a rectangular structure, and round corners (10) are machined at four edges and corners of the base plate (1).
5. The mycotoxin test paper of claim 1, wherein: four corners of bottom plate (1) upper surface all fixedly connected with rubber head (6), rubber head (6) cross section is circular, four corners of bottom plate (1) lower surface all set up with rubber head (6) matched with locating hole (12), locating hole (12) internal diameter is the same with rubber head (6) external diameter.
6. The mycotoxin test paper of claim 5, wherein: blind holes are formed in the positions, where the rubber heads (6) are arranged, of the bottom plate (1), and one ends of the rubber heads (6) are stuck in the blind holes.
7. The mycotoxin test paper of claim 1, wherein: a film (11) is fixed at the edge of the upper part of one side surface in the strip-shaped groove (4), and the other end of the film (11) is fixedly connected with the test strip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223490872.XU CN219935684U (en) | 2022-12-27 | 2022-12-27 | Mycotoxin detection test paper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223490872.XU CN219935684U (en) | 2022-12-27 | 2022-12-27 | Mycotoxin detection test paper |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219935684U true CN219935684U (en) | 2023-10-31 |
Family
ID=88502545
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202223490872.XU Active CN219935684U (en) | 2022-12-27 | 2022-12-27 | Mycotoxin detection test paper |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219935684U (en) |
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2022
- 2022-12-27 CN CN202223490872.XU patent/CN219935684U/en active Active
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| GR01 | Patent grant | ||
| GR01 | Patent grant |