CN212964984U - PCT antibody test strip and test device composed of same - Google Patents
PCT antibody test strip and test device composed of same Download PDFInfo
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- CN212964984U CN212964984U CN202021794540.9U CN202021794540U CN212964984U CN 212964984 U CN212964984 U CN 212964984U CN 202021794540 U CN202021794540 U CN 202021794540U CN 212964984 U CN212964984 U CN 212964984U
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Abstract
The utility model discloses a PCT antibody test strip and testing arrangement who constitutes thereof, comprising a base plate, the sample pad, mark pad and nitrocellulose membrane connect gradually and fixed the setting at the bottom plate upside, the nitrocellulose membrane is gone up the parcel and is had the antigen and the immunoglobulin that the genetic engineering antibody that awaits measuring corresponds, the mark pad coating has two anti marker antibodies of first two anti marker antibodies and second, two anti marker antibodies of first two anti marker antibodies are the two anti marker conjugates of the genetic engineering antibody that awaits measuring, the second is two anti marker antibodies of second for the two marker conjugates of immunoglobulin. The detection among different experimental projects can be realized independently and synchronously at any time, and the number of the experimental projects is not limited generally. Use the utility model discloses, save the experimental time, check-out time can be shortened to 10~15min from 1.5h ~ 2.5h of original ELISA method.
Description
Technical Field
The utility model relates to a biological assay device technical field specifically is a PCT antibody test strip and testing arrangement who constitutes thereof.
Background
At present, enzyme-linked immunosorbent assay (ELISA) is generally adopted and the detection principle of indirect method is utilized to detect the expression condition of the cell supernatant of the genetically engineered antibody. The ELISA method has more test steps during detection and use, has longer detection time, requires certain skill for experimental operation, and has relatively complex detection process.
At present, the liquid chromatograph and the detection system formed by the liquid chromatograph can also be used for the concentration or titer and quantity of genetically engineered antibodies in cell supernatant. Firstly, the liquid chromatograph and the detection system formed by the liquid chromatograph are not specially used for detecting the expression condition of the cell supernatant of the genetic engineering antibody, and the level of the detection system in the field of detecting macromolecules is not high; secondly, the liquid chromatograph and the detection system composed of the liquid chromatograph are generally used for analyzing the precise chemical composition components, so the price is high, and the requirements of the instruments and equipment on experimental operation are higher.
Therefore, the skilled in the art needs a simple and easy-to-operate device that can accurately detect the concentration or titer and amount of the genetically engineered antibody in the cell supernatant.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a PCT antibody test strip and testing arrangement who constitutes thereof to solve the problem among the prior art.
In order to realize the purpose of the above aspect, the utility model provides the following technical scheme:
a PCT antibody test strip comprises a bottom plate, a sample pad, a marking pad and a nitrocellulose membrane, wherein the sample pad, the marking pad and the nitrocellulose membrane are sequentially connected and fixedly arranged on the upper side of the bottom plate,
the cellulose nitrate membrane is coated with an antigen and immunoglobulin corresponding to a genetic engineering antibody to be detected, the marking pad is coated with a first secondary anti-marker antibody and a second secondary anti-marker antibody, the first secondary anti-marker antibody is a secondary anti-marker conjugate of the genetic engineering antibody to be detected, and the second secondary anti-marker antibody is a secondary anti-marker conjugate of the immunoglobulin.
In order to more accurately express the method, the detection sample contains the genetic engineering antibody to be detected when the method is used, and the detection sample refers to the antibody to be detected produced by utilizing the genetic engineering technology, and generally is mostly from mice; the antibody of the genetic engineering antibody to be detected is a second antibody which is of another animal source and can resist the genetic engineering antibody to be detected, such as a goat anti-mouse IgG antibody; the secondary antibody labeled conjugate of the genetic engineering antibody to be detected is prepared by labeling and combining a secondary antibody with colloidal gold or other labeled substances to finally form a secondary antibody labeled conjugate.
Preferably, the upper side of the bottom plate is also connected with water absorption filter paper, and the water absorption filter paper is connected with the nitrocellulose membrane.
Preferably, the sample pad, the labeling pad and the nitrocellulose membrane are sequentially connected in a lap joint manner.
Furthermore, the lapping part of the sample pad and the marking pad is fixedly connected, the width is 0.5-5 mm,
and/or the marking pad is fixedly connected with the lapping part of the nitrocellulose membrane, and the width is 0.5-5 mm.
In order to achieve the above object, the present invention provides the following technical solutions:
a test device for rapidly detecting the expression level of cell supernatants of genetically engineered antibodies comprises a plastic card shell, a PCT antibody test strip and a colorimetric card, wherein the PCT antibody test strip is any one of the PCT antibody test strips.
The test strip box is characterized in that a plurality of clamping grooves for placing the PCT antibody test strips are formed in the plastic card shell, correspond to the clamping grooves, and are provided with a plurality of result display windows and sample adding holes.
Preferably, the colorimetric card has a plurality of detection control lines which gradually increase from shallow to deep.
The utility model discloses there is following beneficial effect:
the utility model discloses a PCT antibody test strip and testing arrangement who constitutes thereof connects gradually and fixes the setting at the bottom plate upside through sample pad, mark pad and nitrocellulose membrane, and the nitrocellulose membrane is upwrapped to have antigen and the immunoglobulin that the genetic engineering antibody that awaits measuring corresponds, and the mark pad coating has two anti-marker antibodies of first two anti-marker antibody and second, realizes that the detection between the different experimental projects can carry out by mutual independence, can go on in step at any time. Also, the number of experimental items is generally not limited, as with antibody test strips corresponding to different items.
Use the utility model discloses a PCT antibody test strip and testing arrangement who constitutes thereof still saves the experimental time, and with regard to sample detection, the check-out time can be followed 1.5h ~ 2.5h of original ELISA method and is unequal to shorten to present 10~15min about. The saved experimental time is more significant if the items tested or the cell supernatants are more heterogeneous.
Use the utility model discloses a PCT antibody test strip and testing arrangement who constitutes thereof still need not rely on imported instrument and equipment, reduces restrictive condition and factor, suitably practices thrift the cost spending of experiment or project.
Drawings
Fig. 1 is a schematic structural diagram of a PCT antibody test strip of the present invention;
FIG. 2 is a schematic structural diagram of a testing device for rapidly detecting the expression level of the cell supernatant of the genetically engineered antibody according to the present invention;
FIG. 3 is a schematic structural diagram of another embodiment of the testing device for rapid detection of the expression level of the cell supernatant of the genetically engineered antibody according to the present invention;
FIG. 4 is a schematic structural diagram of a color chart of the device for rapidly detecting the expression level of the cell supernatant of the genetically engineered antibody of the present invention;
in the figure: 1-bottom plate, 2-sample pad, 3-marking pad, 4-nitrocellulose membrane, 5-absorbent filter paper, 6-plastic card shell, 7-result display window, 8-sample adding hole and 9-colorimetric card.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In order to more accurately express the method, the detection sample contains the genetic engineering antibody to be detected when the method is used, and the detection sample refers to the antibody to be detected produced by utilizing the genetic engineering technology, and generally is mostly from mice; the antibody of the genetic engineering antibody to be detected is a second antibody which is of another animal source and can resist the genetic engineering antibody to be detected, such as a goat anti-mouse IgG antibody; the secondary antibody labeled conjugate of the genetic engineering antibody to be detected is prepared by labeling and combining a secondary antibody with colloidal gold or other labeled substances to finally form a secondary antibody labeled conjugate.
Referring to fig. 1 to 4, a PCT antibody test strip includes a base plate 1, a sample pad 2, a label pad 3, and a nitrocellulose membrane 4, wherein the sample pad 2, the label pad 3, and the nitrocellulose membrane 4 are sequentially connected and fixedly disposed on the upper side of the base plate 1,
the nitrocellulose membrane 4 is coated with an antigen and immunoglobulin corresponding to a genetic engineering antibody to be detected, the marker pad 3 is coated with a first secondary anti-marker antibody and a second secondary anti-marker antibody, the first secondary anti-marker antibody is a secondary marker conjugate of the genetic engineering antibody to be detected, and the second secondary anti-marker antibody is a secondary marker conjugate of the immunoglobulin.
Preferably, the upper side of the bottom plate 1 is further connected with a water absorption filter paper 5, and the water absorption filter paper 5 is connected with the nitrocellulose membrane 4.
Preferably, the sample pad 2, the label pad 3 and the nitrocellulose membrane 4 are sequentially connected in an overlapping manner.
Furthermore, the lapping part of the sample pad 2 and the marking pad 3 is fixedly connected, the width is 0.5-5 mm,
and/or the mark pad 3 is fixedly connected with the lap joint part of the nitrocellulose membrane 4, and the width is 0.5-5 mm.
A test device for rapidly detecting the expression level of cell supernatants of genetically engineered antibodies comprises a plastic card shell 6, a PCT antibody test strip and a colorimetric card 9, wherein the PCT antibody test strip is the PCT antibody test strip of any one of the above items,
a plurality of clamping grooves for placing the PCT antibody test strips are formed in the plastic card shell 6 and correspond to the clamping grooves, and a plurality of result display windows 7 and sample adding holes 8 are formed in the plastic card shell 6.
Preferably, the colorimetric card 9 has a plurality of detection control lines which gradually increase from shallow to deep.
The nitrocellulose membrane 4 (also referred to as NC membrane) is coated with an antigen corresponding to the genetically engineered antibody to be tested, and immunoglobulin derived from another animal species used for the quality control system. A first secondary anti-marker antibody and a second secondary anti-marker antibody are sprayed on the marking pad 3, the first secondary anti-marker antibody is a secondary marker conjugate of the genetic engineering antibody to be detected, the second secondary anti-marker antibody is a secondary marker conjugate of the immunoglobulin, the first secondary anti-marker antibody can be specifically a secondary marker conjugate of the genetic engineering antibody to be detected, and the second secondary anti-marker antibody can be a secondary marker antibody of the quality control system, which is directed against the immunoglobulin of another animal species source on the NC membrane.
The PCT antibody test strip is obtained by adhering a sample pad 2, a marking pad 3, a nitrocellulose membrane 4 and absorbent filter paper 5 on a bottom plate 1 through self-adhesive.
A test device for rapidly detecting the expression level of the cell supernatant of a genetically engineered antibody consists of a PCT antibody test strip, a plastic card shell 6 can be divided into an upper card shell and a lower card shell, the plastic card shell 6 is provided with a clamping groove, and the PCT antibody test strip is placed in the clamping groove, so that the PCT antibody test strip is fixed. The test strip can be designed according to the needs, and a PCT antibody test strip or a plurality of PCT antibody test strips can be placed in the plastic card shell.
A test device for rapidly detecting the expression level of cell supernatants of genetic engineering antibodies is also provided with a colorimetric card 9 on which color development lines with different color development degrees and grades between G1-G12 are printed for comparing the color development intensity of the detection lines of a detected sample. And comparing with the result of the reference substance to obtain the expression level of the cell supernatant in the sample.
When using, the experimenter will the utility model discloses a testing arrangement is prepared in advance, including preparing plastics card shell 6, PCT antibody test strip, colour comparison card 9 to fix PCT antibody test strip in plastics card shell 6. The PCT antibody test strip is fixed with a sample pad 2, a nitrocellulose membrane 4 (coated with a scribing membrane), a marking pad 3 (sprayed with a marking antibody) and absorbent filter paper 5 in advance, and the sample pad 2, the nitrocellulose membrane 4 (coated with the scribing membrane), the marking pad 3 (sprayed with the marking antibody) and the absorbent filter paper 5 are sequentially adhered to a bottom plate by using self-adhesive. Control (also referred to as standard) antibodies at different concentration gradients were then prepared, as well as cell supernatant samples to be tested. The antibody of a reference substance (also called as a standard substance) and the sample to be tested respectively pass through the sample adding hole 8 of the testing device and are dripped on the sample pad 2 of the corresponding PCT antibody test strip, and then timing is started, and the liquid passes through the sample pad 2, the marking pad 3, the nitrocellulose membrane 4 and the absorbent filter paper 5 along the PCT antibody test strip in sequence. Generally, the color comparison can be judged within 10-15 min, the line results of the concentration gradient reference samples are sequentially compared by using a color comparison card 9, and the color development intensity is recorded. And comparing the test line results of the samples to be tested by using a colorimetric card 9, and recording the color development intensity. Then, the two are compared, and the result of the sample to be detected is judged to be close to the result of the reference substance with which concentration gradient the color development intensity of the sample to be detected is, namely, the color development intensity of the sample to be detected is approximate to the concentration (or concentration range) of the antibody in the supernatant of the cell to be detected, so as to estimate the expression level of the antibody.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (6)
1. A PCT antibody test strip is characterized by comprising a bottom plate (1), a sample pad (2), a marking pad (3) and a nitrocellulose membrane (4), wherein the sample pad (2), the marking pad (3) and the nitrocellulose membrane (4) are sequentially connected and fixedly arranged on the upper side of the bottom plate (1),
the cellulose nitrate membrane (4) is coated with an antigen and immunoglobulin corresponding to a genetic engineering antibody to be detected, the marking pad (3) is coated with a first secondary anti-marker antibody and a second secondary anti-marker antibody, the first secondary anti-marker antibody is a secondary anti-marker conjugate of the genetic engineering antibody to be detected, and the second secondary anti-marker antibody is a secondary anti-marker conjugate of the immunoglobulin.
2. The PCT antibody test strip according to claim 1, wherein a water absorbent filter paper (5) is further attached to the upper side of the base plate (1), and the water absorbent filter paper (5) is attached to the nitrocellulose membrane (4).
3. The PCT antibody test strip of claim 1, wherein the sample pad (2), the label pad (3) and the nitrocellulose membrane (4) are sequentially lapped.
4. The PCT antibody test strip according to claim 3, characterized in that the sample pad (2) is fixedly connected with the lapping part of the mark pad (3) and the width is 0.5-5 mm,
and/or the mark pad (3) is fixedly connected with the lap joint part of the nitrocellulose membrane (4), and the width is 0.5-5 mm.
5. A test device for rapidly detecting the expression level of cell supernatant of genetically engineered antibody, which is characterized by comprising a plastic card shell (6), a PCT antibody test strip and a colorimetric card (9), wherein the PCT antibody test strip is the PCT antibody test strip of any one of claims 1 to 4,
the PCT antibody test strip detection device is characterized in that a plurality of clamping grooves for placing the PCT antibody test strips are formed in the plastic clamping shell (6) and correspond to the clamping grooves, and a plurality of result display windows (7) and sample adding holes (8) are formed in the plastic clamping shell (6).
6. The test device according to claim 5, wherein the color chart (9) has a plurality of test control lines which gradually increase from shallow to deep.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021794540.9U CN212964984U (en) | 2020-08-25 | 2020-08-25 | PCT antibody test strip and test device composed of same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021794540.9U CN212964984U (en) | 2020-08-25 | 2020-08-25 | PCT antibody test strip and test device composed of same |
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| Publication Number | Publication Date |
|---|---|
| CN212964984U true CN212964984U (en) | 2021-04-13 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021794540.9U Active CN212964984U (en) | 2020-08-25 | 2020-08-25 | PCT antibody test strip and test device composed of same |
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| Country | Link |
|---|---|
| CN (1) | CN212964984U (en) |
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- 2020-08-25 CN CN202021794540.9U patent/CN212964984U/en active Active
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