CN208239466U - A kind of immunofluorescence technique kit detecting folic acid - Google Patents
A kind of immunofluorescence technique kit detecting folic acid Download PDFInfo
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- CN208239466U CN208239466U CN201820863319.0U CN201820863319U CN208239466U CN 208239466 U CN208239466 U CN 208239466U CN 201820863319 U CN201820863319 U CN 201820863319U CN 208239466 U CN208239466 U CN 208239466U
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Abstract
The utility model discloses a kind of immunofluorescence technique kits for detecting folic acid, including kit body and the test strips being set in kit body, kit body includes upper housing, lower case, and it is set to the protection cap on upper housing, test strips are fixed in lower case, test strips include bottom plate, nitrocellulose filter, sample pad, water absorption pad, glass fibre element film, plate upper surface overlaps nitrocellulose filter, glass fibre element film is overlapped on one end of nitrocellulose filter, water absorption pad is overlapped on the other end of nitrocellulose filter, sample pad is overlapped on glass fibre element film, there are the detection line and the coated nature controlling line of sheep anti-mouse igg antibody of folic acid-BSA antigen coat on nitrocellulose filter, the folic acid monoclonal antibody I of rare-earth fluorescent microballoon label is coated on the glass fibre element film；Using the utility model prepare kit carry out folic acid detection when, it is easy to operate, quick and precisely, reproducible, data are reliable, provide great convenience for clinical use.
Description
Technical field
The utility model relates to Immunofluorescence test technical fields, and in particular to a kind of immunofluorescence technique examination for detecting folic acid
Agent box.
Background technique
The compound that folic acid (Folic Acid) is made of petrin pyridine, p-aminobenzoic acid and glutamic acid etc. is a kind of
Water-soluble B vitamin.Folic acid has an important trophism to human body, and macrocytic anemia and white thin can be caused by lacking folic acid
Born of the same parents reduce disease, also result in weak, irritability, have no appetite and psychotic symptoms.In addition, folic acid is even more important to pregnant woman,
Such as lack folic acid in pregnancy head 3 months, can lead to fetal neural tube developmental defect, to increase the hair for splitting animal brains, anencephalus
Raw rate.The effect of present folic acid is just gradually recognized by people, and many countries add in people's diet consciously
Folic acid, the Ministry of Public Health on June 21 in 2009 announce that 6 public health programs of starting, supplement folic acid prevent neural tube defect project, this
Just higher requirement is proposed for the detection of folic acid.
Folic acid detection method includes: enzyme-linked immunoassay method, gas chromatography mass spectrometry (GC-MS), liquid chromatography tandem at present
Mass spectrography (LC-MS-MS), liquid chromatography, colorimetric method, thin layer chromatography, microbial method and isotope radioimmunology etc., instrument
Device method is sensitive, accurate, and high specificity, separating degree are good, can measure a variety of drugs simultaneously, but needs expensive instrument, sample
Complex pretreatment, cumbersome time-consuming, detection higher cost, are unable to execute-in-place, and professional is needed to operate, so limiting
It is applied, and enzyme-linked immunoassay method has detection device with high requirements and high cost；Disturbing factor is more, and repeatability is bad；Detection time
The disadvantages of long, it is therefore necessary to research and develop a kind of fast and accurately detection method and means, facilitate total evaluation pregnant woman
Health status.
Utility model content
The purpose of this utility model is to provide a kind of immunofluorescence technique kit for detecting folic acid, high sensitivities, stabilization
Property it is good, sampling is convenient, is conducive in time carry out pregnant woman whole effective assessment.
To achieve the above object, the utility model provides the following technical solutions: a kind of immunofluorescence technique examination detecting folic acid
Agent box, including kit body and the test strips being set in kit body；The kit body include upper housing, under
Shell and the protection cap being set on upper housing, the side of the upper housing are equipped with the first groove, and the is equipped in the first groove
One sliding rail, protection cap are equipped with the first sliding block, the first connecting plate, the second connecting plate, and the first sliding block is located in the first sliding rail, and first
Sliding block is matched with the first sliding rail, and the both ends of the first sliding block along its length are equipped with cylindrical fixture block, and the both ends of the first sliding rail are equal
Equipped with cylindrical card slot, cylindrical card slot is matched with cylindrical fixture block, and the first connecting plate is strip, and the second connecting plate is cut
Face is " n " character form structure, and one end of the first connecting plate is connected with the first sliding block, the other end of the first connecting plate and the second connecting plate
It is connected, the first connecting plate, the first sliding block are respectively positioned in the first groove；Upper housing upper surface is equipped with well, detection window, sample-adding
Hole, detection window are the through-hole across upper housing, and upper housing inner surface is equipped with several card bodies, two strips, and two strips are located at
Detect the upper and lower ends of window, the width of two strips and test strips it is of same size, lower case inner surface be equipped with the first positive stop strip,
Second positive stop strip and card slot corresponding with upper housing card body, the first positive stop strip are " n " character form structure, the first positive stop strip packet
The first vertical arm, the first transverse arm, the second vertical arm are included, the distance between the first vertical arm and the second vertical arm are of same size with test strips,
Second positive stop strip is string configuration, and the distance between two second positive stop strips are of same size with test strips, two first limits
The distance between first transverse arm of position item is identical as the length of test strips.
Above-mentioned a kind of immunofluorescence technique kit for detecting folic acid, wherein second connecting plate is along length direction
Both ends be equipped with baffle straight down, baffle is silica gel material, the height of baffle be the second connecting plate to upper housing upper surface it
Between distance.
Above-mentioned a kind of immunofluorescence technique kit for detecting folic acid, wherein the kit body is equipped with anti-card hole, prevents
Card hole is long stripes through hole.
Above-mentioned a kind of immunofluorescence technique kit for detecting folic acid, wherein the test strips include bottom plate, nitrocellulose
Film, sample pad, water absorption pad, glass fibre element film, the plate upper surface overlap nitrocellulose filter, glass fibre element film overlap joint
In one end of nitrocellulose filter, water absorption pad is overlapped on the other end of nitrocellulose filter, and sample pad is overlapped in glass fibre element
On film, there are the detection line and the coated nature controlling line of sheep anti-mouse igg antibody of folic acid-BSA antigen coat on the nitrocellulose filter,
The folic acid monoclonal antibody I of rare-earth fluorescent microballoon label is coated on the glass fibre element film, rare-earth fluorescent microballoon is with dilute
Earth elements europium is the latex particle of fluorescent matrix material.
Above-mentioned a kind of immunofluorescence technique kit for detecting folic acid, wherein the detection line and nature controlling line are parallel to each other, inspection
Survey line is close to sample pad, and nature controlling line is close to water absorption pad.
Above-mentioned a kind of immunofluorescence technique kit for detecting folic acid, wherein the diameter of the rare-earth fluorescent microballoon is
100nm-300nm。
The utility model additionally provides a kind of method for preparing above-mentioned test strips, includes the following steps:
(1) it prepares glass fibre element film: BBS buffer (pH=7.2) of the glass fibre element film containing polyvinyl alcohol is soaked
Bubble is handled, and after immersion treatment, 37 DEG C are dried overnight, and are resisted the folic acid monoclonal that rare-earth fluorescent microballoon marks using quantitative spray film instrument
Body I is sprayed on glass fibre element film, and drying 4 hours is protected from light at 37 DEG C, and addition desiccant is sealed up for safekeeping spare；
(2) it prepares nitrocellulose filter: nitrocellulose filter is put into dryer, 25-37 DEG C of drying process 4-10h,
It is put into 20-30 DEG C after drying, is protected from light processing 2-4h in the environment of humidity 50-70%, is drawn using film instrument is drawn in detection line position
Folic acid-BSA antigen draws upper sheep anti-mouse igg antibody in Quality Control line position, drying 4 hours is protected from light at 37 DEG C, desiccant is added and seals up for safekeeping
It is spare；
(3) it assembles: overlapping nitrocellulose filter, glass fibre element film, sample pad, water absorption pad on bottom plate, then carry out
Cutting, is fitted into kit body.
Wherein, the folic acid monoclonal antibody of rare-earth fluorescent microballoon label I's the preparation method is as follows: by folic acid monoclonal
Antibody I is dissolved in the PBS buffer solution (pH=7.2) of 0.05mol/L, obtains folic acid monoclonal antibody I solution, folic acid monoclonal is resisted
Body I solution, which is transferred in super filter tube, to be purified, and carries out 3-6 flushing with the PBS buffer solution of 0.05mol/L (pH=7.2),
The folic acid monoclonal antibody I purified washs rare-earth fluorescent microballoon using 0.05mol/L MES buffer (pH=7.2), adds
Enter carbodiimide and n-hydroxysuccinimide, reacts at room temperature 1h, centrifugal filtration, with the PBS buffer solution (pH=of 0.05mol/L
7.2) the folic acid monoclonal antibody I of purifying is added after redissolving, reacts at room temperature 5 hours, is added the 0.01mol/L's containing 1%BSA
PBS buffer solution (pH=7.2) reacts at room temperature 1h, washs rare-earth fluorescent microballoon with the PBS buffer solution (pH=7.2) of 0.01mol/L
3-5 times, finally with 0.05%BSA is contained, the PBS buffer solution (pH=7.2) of 0.05%Tween-20,0.01mol/L are saved.
Wherein, the folic acid-BSA antigen the preparation method is as follows: BSA and folic acid to be dissolved in the PBS of 0.05mol/L respectively
It in buffer (pH=7.2), stirs evenly, respectively obtains BSA solution and folic acid solution, it is sub- that carbon two is added in folic acid solution
Amine adds BSA solution after mixing evenly, is protected from light 2 hours, obtains folic acid-BSA antigenic solution, by folic acid-BSA antigen
Solution is transferred in super filter tube and is purified, and carries out 3-5 flushing with the PBS buffer solution of 0.05mol/L (pH=7.2), receives
Collect folic acid-BSA antigen.
When use: sample liquid being added dropwise in sample pad, under capillary action, sample liquid works as sample liquid to water absorption pad swimming
In contain folic acid when, folic acid and rare-earth fluorescent microballoon label folic acid monoclonal antibody I formed antigen-antibody complex, with
Chromatography effect, compound move forward, and when reaching detection line, with folic acid-BSA antigen binding, it is multiple to form antigen-antibody-antigen
Close object, the antigen-antibody complex of unbonded folic acid-BSA antigen continues to move ahead, when reaching nature controlling line, sheep anti-mouse igg antibody with
Antigen-antibody complex combines, and entire reaction is completed, upper machine-read card.Detection line and nature controlling line can all generate corresponding fluorescence letter
Number, actually detected value can be brought into according to the information on calibration card and calculate quantitative knot in preset standard curve by fluorescence detector
Fruit.
The utility model have the utility model has the advantages that
(1) the utility model kit body is equipped with protection cap, can live well, detection window cover, make when unused
Used time can be removed protection cap by the cooperation of sliding rail and sliding block from well, detection window, then be detected, and effectively be kept away
Caused by having exempted from because of misoperation the occurrence of cross contamination；The setting of upper housing inner surface, lower case inner surface can incite somebody to action simultaneously
Test strips are stably fixed in kit body, and kit is not in that test strips fall off, deviate or inhale during transportation
The situation of tide occurs, easy to operate when carrying out sample detection using kit prepared by the utility model, quick and precisely, repeats
Property is good, and data are reliable；
(2) time resolution immunochromatography technique is introduced the inspection of folic acid by improving to test strips by the utility model
In survey, using rare-earth fluorescent microballoon as marker, thousands of a fluorescent molecules can be wrapped up in each microballoon, are substantially increased
Labeling effciency effectively raises sensitivity, while fluorescent microsphere surface modification has the carboxyl of proper density, for albumen or
The covalent coupling of antibody improves the stability of marker, furthermore high sensitivity, good linearity, batch in, difference between batch it is small, make for clinic
With providing great convenience.
Detailed description of the invention
FIG. 1 is a schematic structural view of the utility model；
Fig. 2 is upper housing inner surface schematic diagram；
Fig. 3 is lower case inner surface schematic diagram；
Fig. 4 is that test strips schematic diagram is packed into lower case；
Fig. 5 is the right view of Fig. 1；
Fig. 6 is protection cap schematic diagram；
Fig. 7 is test strips structure schematic diagram；
Fig. 8 is the canonical plotting of embodiment 2；
Fig. 9 is the canonical plotting of embodiment 3；
In figure: 1- kit body, 2- test strips, 3- upper housing, 4- lower case, 5- protection cap, the first groove of 6-, 7-
One sliding rail, the first sliding block of 8-, the first connecting plate of 9-, the second connecting plate of 10-, 11- cylinder fixture block, 12- well, 13- detection
Window, 14- card body, 15- strip, the first positive stop strip of 16-, the second positive stop strip of 17-, 18- card slot, the first vertical arm of 19-, 20- first are horizontal
Arm, the second vertical arm of 21-, 22- baffle, the anti-card hole of 23-, 24- bottom plate, 25- nitrocellulose filter, 26- sample pad, 27- water absorption pad,
28- glass fibre element film, 29- detection line, 30- nature controlling line.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe.
Embodiment 1
The present embodiment elaborates the structure of the immunofluorescence technique kit of detection folic acid.
As shown in figs. 1-7, a kind of immunofluorescence technique kit detecting folic acid, including kit body 1 and be set to
Test strips 2 in kit body；The kit body includes upper housing 3, lower case 4 and is set on upper housing
Protection cap 5, the side of the upper housing 3 are equipped with the first groove 6, are equipped with the first sliding rail 7 in the first groove 6, protection cap 5 is equipped with
First sliding block 8, the first connecting plate 9, the second connecting plate 10, the first sliding block 8 are located in the first sliding rail 7, and the first sliding block 8 and first is sliding
Rail 7 matches, and the first sliding block 8 is equipped with cylindrical fixture block 11 along the both ends in length direction, and the both ends of the first sliding rail 7 are equipped with
Cylindrical card slot, cylindrical card slot are matched with cylindrical fixture block, and the first connecting plate 9 is string configuration, the second connecting plate 10
Section be " n " character form structure, one end of the first connecting plate 9 is connected with the first sliding block 8, the other end of the first connecting plate 9 and the
Two connecting plates 10 are connected, and the first connecting plate 9, the first sliding block 8 are respectively positioned in the first groove 6；1 upper surface of upper housing is equipped with well
12, detect window 13, well 12, detection window 13 are through-hole across upper housing, upper housing inner surface be equipped with several card bodies 14,
Two strips 15, two strips 15 are located at the upper and lower ends of detection window 13, the width and the width phase of test strips 2 of two strips 15
Together, 3 inner surface of lower case is equipped with the first positive stop strip 16, the second positive stop strip 17 and card slot 18 corresponding with upper housing card body,
First positive stop strip 16 is " n " character form structure, and the first positive stop strip 16 includes the first vertical arm 19, the first transverse arm 20, the second vertical arm 21, the
The distance between one vertical arm 19 and the second vertical arm 21 are of same size with test strips 2, the second positive stop strip 17 be string configuration, two
The distance between a second positive stop strip 17 is of same size with test strips 2, between the first transverse arm 20 of two the first positive stop strips
Distance is identical as the length of test strips 2.
Above-mentioned a kind of immunofluorescence technique kit for detecting folic acid, wherein second connecting plate 10 is along length side
To both ends be equipped with baffle 22 straight down, baffle 22 is silica gel material, and the height of baffle 22 is that the second connecting plate 19 arrives upper casing
The distance between 3 upper surface of body.
Above-mentioned a kind of immunofluorescence technique kit for detecting folic acid, wherein the kit body 1 is equipped with anti-card hole
23, anti-card hole 23 is long stripes through hole.
Above-mentioned a kind of immunofluorescence technique kit for detecting folic acid, wherein the test strips 2 include bottom plate 24, nitric acid fibre
Plain film 25, sample pad 26, water absorption pad 27, glass fibre element film 28 are tieed up, 24 upper surface of bottom plate overlaps nitrocellulose filter 25,
Glass fibre element film 28 is overlapped on one end of nitrocellulose filter 25, and water absorption pad 27 is overlapped on the another of nitrocellulose filter 25
End, sample pad 26 are overlapped on glass fibre element film 28, there is the inspection of folic acid-BSA antigen coat on the nitrocellulose filter 25
Survey line 29 and sheep anti-mouse igg antibody coated nature controlling line 30 are coated with rare-earth fluorescent microballoon mark on the glass fibre element film 28
The folic acid monoclonal antibody I of note, rare-earth fluorescent microballoon are using rare earth elements europium as the latex particle of fluorescent matrix material.
Above-mentioned a kind of immunofluorescence technique kit for detecting folic acid, wherein the detection line 29 and nature controlling line 30 are mutually flat
Row, detection line 29 is close to sample pad 26, and nature controlling line 30 is close to water absorption pad 27.
Above-mentioned a kind of immunofluorescence technique kit for detecting folic acid, wherein the diameter of the rare-earth fluorescent microballoon is
100nm-300nm。
Embodiment 2
The present embodiment illustrates the preparation method of the immunofluorescence technique test strips of detection folic acid.
A kind of preparation method for the immunofluorescence technique test strips detecting folic acid, includes the following steps:
(1) it prepares glass fibre element film: BBS buffer (pH=7.2) of the glass fibre element film containing polyvinyl alcohol is soaked
Bubble is handled, and after immersion treatment, 37 DEG C are dried overnight, and are resisted the folic acid monoclonal that rare-earth fluorescent microballoon marks using quantitative spray film instrument
Body I is sprayed on glass fibre element film, and drying 4 hours is protected from light at 37 DEG C, and addition desiccant is sealed up for safekeeping spare；
(2) it prepares nitrocellulose filter: nitrocellulose filter is put into dryer, 25 DEG C of drying process 4h, after dry
It is put into 25 DEG C, is protected from light processing 4h in the environment of humidity 50%, the PBS buffer solution (pH=7.2) of 0.05mol/L is used to adjust leaf
The final concentration of 1.2mg/ml of acid-BSA antigen and sheep anti-mouse igg antibody draws upper folic acid-in detection line position using film instrument is drawn
BSA antigen draws upper sheep anti-mouse igg antibody in Quality Control line position, drying 4 hours is protected from light at 37 DEG C, and addition desiccant is sealed up for safekeeping spare；
(3) it assembles: overlapping nitrocellulose filter, glass fibre element film, sample pad, water absorption pad on bottom plate, then carry out
Cutting, is fitted into kit body.
Wherein, the folic acid monoclonal antibody of rare-earth fluorescent microballoon label I's the preparation method is as follows: by folic acid monoclonal
Antibody I is dissolved in the PBS buffer solution (pH=7.2) of 0.05mol/L, obtains folic acid monoclonal antibody I solution, folic acid monoclonal is resisted
Body I solution, which is transferred in super filter tube, to be purified, and carries out 3-6 flushing with the PBS buffer solution of 0.05mol/L (pH=7.2),
The concentration of the folic acid monoclonal antibody I purified, adjustment folic acid monoclonal antibody I are 1.2mg/ml, use 0.05mol/L
MES buffer (pH=7.2) washs rare-earth fluorescent microballoon, and carbodiimide and n-hydroxysuccinimide is added, and reacts at room temperature 1h,
Centrifugal filtration is added the folic acid monoclonal antibody I of purifying, makes leaf after being redissolved with the PBS buffer solution (pH=7.2) of 0.05mol/L
The mass ratio of sour monoclonal antibody I and rare-earth fluorescent microballoon is 1:40, is reacted at room temperature 5 hours, and addition contains 1%BSA's
The PBS buffer solution (pH=7.2) of 0.01mol/L reacts at room temperature 1h, is washed with the PBS buffer solution (pH=7.2) of 0.01mol/L
Rare-earth fluorescent microballoon 5 times, finally with containing 0.05%BSA, the PBS buffer solution (pH=of 0.05%Tween-20,0.01mol/L
7.2) it saves.
Wherein, the folic acid-BSA antigen the preparation method is as follows: BSA and folic acid to be dissolved in the PBS of 0.05mol/L respectively
It in buffer (pH=7.2), stirs evenly, respectively obtains BSA solution and folic acid solution, it is sub- that carbon two is added in folic acid solution
Amine adds BSA solution after mixing evenly, is protected from light 2 hours, obtains folic acid-BSA antigenic solution, by folic acid-BSA antigen
Solution is transferred in super filter tube and is purified, and carries out 5 flushings with the PBS buffer solution of 0.05mol/L (pH=7.2), collects
Folic acid-BSA antigen.
The detection method of above-mentioned test strips:
(7 different concentration are taken, respectively in the folic acid standard items that various concentration is added in the sample application zone of the test strips prepared
For 0ug/L, 1ug/L, 2ug/L, 4ug/L, 10ug/L, 20ug/L, 40ug/L, each concentration does 3 Duplicate Samples, reacts 15 minutes
Afterwards, fluorescence detector reads nature controlling line C, detection line T signal, and specific value is as shown in table 1:
Table 1: the corresponding detection signal data of 2 Plays product concentration of embodiment
Using the fluorescence value signal of detection as ordinate, folic acid standard concentration value is abscissa, draws standard items curve, ginseng
Fig. 8 is read, the R of the standard curve it can be seen from Fig. 8 standard curve2It is 0.999, it is linear preferable, the standard curve can be passed through
Quantitative analysis is carried out to folic acid concentration contained in sample.
Embodiment 3
The present embodiment illustrates the preparation method of the immunofluorescence technique test strips of detection folic acid.
A kind of preparation method for the immunofluorescence technique test strips detecting folic acid, includes the following steps:
(1) it prepares glass fibre element film: BBS buffer (pH=7.2) of the glass fibre element film containing polyvinyl alcohol is soaked
Bubble is handled, and after immersion treatment, 37 DEG C are dried overnight, and are resisted the folic acid monoclonal that rare-earth fluorescent microballoon marks using quantitative spray film instrument
Body I is sprayed on glass fibre element film, and drying 7 hours is protected from light at 37 DEG C, and addition desiccant is sealed up for safekeeping spare；
(2) it prepares nitrocellulose filter: nitrocellulose filter is put into dryer, 37 DEG C of drying process 4h, after dry
It is put into 30 DEG C, is protected from light processing 4h in the environment of humidity 70%, the PBS buffer solution (pH=7.2) of 0.05mol/L is used to adjust leaf
The final concentration of 1.5mg/ml of acid-BSA antigen and sheep anti-mouse igg antibody draws upper folic acid-in detection line position using film instrument is drawn
BSA antigen draws upper sheep anti-mouse igg antibody in Quality Control line position, drying 4 hours is protected from light at 37 DEG C, and addition desiccant is sealed up for safekeeping spare；
(3) it assembles: overlapping nitrocellulose filter, glass fibre element film, sample pad, water absorption pad on bottom plate, then carry out
Cutting, is fitted into kit body.
Wherein, the folic acid monoclonal antibody of rare-earth fluorescent microballoon label I's the preparation method is as follows: by folic acid monoclonal
Antibody I is dissolved in the PBS buffer solution (pH=7.2) of 0.05mol/L, obtains folic acid monoclonal antibody I solution, folic acid monoclonal is resisted
Body I solution, which is transferred in super filter tube, to be purified, and carries out 3-6 flushing with the PBS buffer solution of 0.05mol/L (pH=7.2),
The concentration of the folic acid monoclonal antibody I purified, adjustment folic acid monoclonal antibody I are 1.5mg/ml, use 0.05mol/L
MES buffer (pH=7.2) washs rare-earth fluorescent microballoon, and carbodiimide and n-hydroxysuccinimide is added, and reacts at room temperature 1h,
Centrifugal filtration is added the folic acid monoclonal antibody I of purifying, makes leaf after being redissolved with the PBS buffer solution (pH=7.2) of 0.05mol/L
The mass ratio of sour monoclonal antibody I and rare-earth fluorescent microballoon is 1:30, is reacted at room temperature 5 hours, and addition contains 1%BSA's
The PBS buffer solution (pH=7.2) of 0.01mol/L reacts at room temperature 1h, is washed with the PBS buffer solution (pH=7.2) of 0.01mol/L
Rare-earth fluorescent microballoon 3 times, finally with containing 0.05%BSA, the PBS buffer solution (pH=of 0.05%Tween-20,0.01mol/L
7.2) it saves.
Wherein, the folic acid-BSA antigen the preparation method is as follows: BSA and folic acid to be dissolved in the PBS of 0.05mol/L respectively
It in buffer (pH=7.2), stirs evenly, respectively obtains BSA solution and folic acid solution, it is sub- that carbon two is added in folic acid solution
Amine adds BSA solution after mixing evenly, is protected from light 4 hours, obtains folic acid-BSA antigenic solution, by folic acid-BSA antigen
Solution is transferred in super filter tube and is purified, and carries out 3 flushings with the PBS buffer solution of 0.05mol/L (pH=7.2), collects
Folic acid-BSA antigen.
The detection method of above-mentioned test strips:
(7 different concentration are taken, respectively in the folic acid standard items that various concentration is added in the sample application zone of the test strips prepared
For 0ug/L, 1ug/L, 2ug/L, 4ug/L, 10ug/L, 20ug/L, 40ug/L, each concentration does 3 Duplicate Samples, reacts 15 minutes
Afterwards, fluorescence detector reads nature controlling line C, detection line T signal, and specific value is as shown in table 2:
Table 2: the corresponding detection signal data of 3 Plays product concentration of embodiment
Using the fluorescence value signal of detection as ordinate, folic acid standard concentration value is abscissa, draws standard items curve, ginseng
Fig. 9 is read, the R of the standard curve it can be seen from Fig. 9 standard curve2It is 0.998, it is linear preferable, the standard curve can be passed through
Quantitative analysis is carried out to folic acid concentration contained in sample.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired
Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.
Claims (6)
1. a kind of immunofluorescence technique kit for detecting folic acid, which is characterized in that including kit body and be set to reagent
The intracorporal test strips of box box；The kit body includes upper housing, lower case and the protection cap being set on upper housing,
The side of the upper housing is equipped with the first groove, and the first sliding rail is equipped in the first groove, and protection cap is equipped with the first sliding block, first
Connecting plate, the second connecting plate, the first sliding block are located in the first sliding rail, and the first sliding block is matched with the first sliding rail, the first sliding block edge
The both ends of length direction are equipped with cylindrical fixture block, and the both ends of the first sliding rail are equipped with cylindrical card slot, cylindrical card slot and cylinder
Shape fixture block matches, and the first connecting plate is strip, and the section of the second connecting plate is " n " character form structure, and the one of the first connecting plate
End is connected with the first sliding block, and the other end of the first connecting plate is connected with the second connecting plate, and the first connecting plate, the first sliding block are respectively positioned on
In first groove；Upper housing upper surface is equipped with well, detection window, and well, detection window are the through-hole across upper housing, on
Shell inner surface is equipped with several card bodies, two strips, and two strips are located at the upper and lower ends of detection window, the width of two strips with
Test strips it is of same size, lower case inner surface is equipped with the first positive stop strip, the second positive stop strip and corresponding with upper housing card body
Card slot, the first positive stop strip be " n " character form structure, the first positive stop strip include the first vertical arm, the first transverse arm, the second vertical arm, first
The distance between vertical arm and the second vertical arm are of same size with test strips, and the second positive stop strip is string configuration, two second limits
The distance between position item of same size with test strips, the distance between first transverse arm of two the first positive stop strips and test strips
Length is identical.
2. a kind of immunofluorescence technique kit for detecting folic acid as described in claim 1, which is characterized in that second connection
Plate is equipped with baffle along the both ends in length direction straight down, and baffle is silica gel material, and the height of baffle is the second connecting plate
The distance between to upper housing upper surface.
3. a kind of immunofluorescence technique kit for detecting folic acid as described in claim 1, which is characterized in that the kit box
Body is equipped with anti-card hole, and anti-card hole is long stripes through hole.
4. a kind of immunofluorescence technique kit for detecting folic acid as described in claim 1, which is characterized in that the test strips packet
Bottom plate, nitrocellulose filter, sample pad, water absorption pad, glass fibre element film are included, the plate upper surface overlaps nitrocellulose
Film, glass fibre element film are overlapped on one end of nitrocellulose filter, and water absorption pad is overlapped on the other end of nitrocellulose filter, sample
Pad is overlapped on glass fibre element film, there is the detection line and sheep anti mouse of folic acid-BSA antigen coat on the nitrocellulose filter
IgG antibody coated nature controlling line is coated with the folic acid monoclonal antibody of rare-earth fluorescent microballoon label on the glass fibre element film
I, rare-earth fluorescent microballoon are using rare earth elements europium as the latex particle of fluorescent matrix material.
5. a kind of immunofluorescence technique kit for detecting folic acid as claimed in claim 4, which is characterized in that the detection line and
Nature controlling line is parallel to each other, and detection line is close to sample pad, and nature controlling line is close to water absorption pad.
6. a kind of immunofluorescence technique kit for detecting folic acid as claimed in claim 4, which is characterized in that the rare-earth fluorescent
The diameter of microballoon is 100nm-300nm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201820863319.0U CN208239466U (en) | 2018-06-05 | 2018-06-05 | A kind of immunofluorescence technique kit detecting folic acid |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201820863319.0U CN208239466U (en) | 2018-06-05 | 2018-06-05 | A kind of immunofluorescence technique kit detecting folic acid |
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| CN208239466U true CN208239466U (en) | 2018-12-14 |
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| CN201820863319.0U Active CN208239466U (en) | 2018-06-05 | 2018-06-05 | A kind of immunofluorescence technique kit detecting folic acid |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108761103A (en) * | 2018-06-05 | 2018-11-06 | 宁波奥丞生物科技有限公司 | A kind of immunofluorescence technique kit of detection folic acid |
| CN113933502A (en) * | 2021-10-19 | 2022-01-14 | 青岛汉唐生物科技有限公司 | Detection card and kit for quantitatively detecting folic acid by immunofluorescence chromatography |
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2018
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108761103A (en) * | 2018-06-05 | 2018-11-06 | 宁波奥丞生物科技有限公司 | A kind of immunofluorescence technique kit of detection folic acid |
| CN113933502A (en) * | 2021-10-19 | 2022-01-14 | 青岛汉唐生物科技有限公司 | Detection card and kit for quantitatively detecting folic acid by immunofluorescence chromatography |
| CN113933502B (en) * | 2021-10-19 | 2024-02-02 | 青岛汉唐生物科技有限公司 | Detection card and kit for quantitatively detecting folic acid by immunofluorescence chromatography |
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