CN219907657U - Thallus culture device for acrylamide production - Google Patents
Thallus culture device for acrylamide production Download PDFInfo
- Publication number
- CN219907657U CN219907657U CN202321285977.3U CN202321285977U CN219907657U CN 219907657 U CN219907657 U CN 219907657U CN 202321285977 U CN202321285977 U CN 202321285977U CN 219907657 U CN219907657 U CN 219907657U
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- China
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- culture tank
- stirring shaft
- air inlet
- top end
- pipe
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- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 title claims abstract description 21
- 238000004519 manufacturing process Methods 0.000 title description 7
- 238000003756 stirring Methods 0.000 claims abstract description 61
- 238000007790 scraping Methods 0.000 claims abstract description 12
- 238000004113 cell culture Methods 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 238000005273 aeration Methods 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 5
- 230000002093 peripheral effect Effects 0.000 claims 1
- 230000000813 microbial effect Effects 0.000 abstract description 15
- 241001052560 Thallis Species 0.000 abstract description 13
- 239000002699 waste material Substances 0.000 abstract description 3
- 230000001580 bacterial effect Effects 0.000 abstract description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 10
- 229910052760 oxygen Inorganic materials 0.000 description 10
- 239000001301 oxygen Substances 0.000 description 10
- 230000000694 effects Effects 0.000 description 6
- 238000006703 hydration reaction Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- NLHHRLWOUZZQLW-UHFFFAOYSA-N Acrylonitrile Chemical compound C=CC#N NLHHRLWOUZZQLW-UHFFFAOYSA-N 0.000 description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 3
- 239000002826 coolant Substances 0.000 description 3
- 229910052802 copper Inorganic materials 0.000 description 3
- 239000010949 copper Substances 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 230000036571 hydration Effects 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000003054 catalyst Substances 0.000 description 2
- 238000006555 catalytic reaction Methods 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108010024026 Nitrile hydratase Proteins 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229910001431 copper ion Inorganic materials 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000010079 rubber tapping Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a bacterial culture device for producing acrylamide, which comprises: a culture tank; a bracket is arranged at the top end of the culture tank; the bracket is rotationally connected with a stirring shaft; the stirring shaft extends downwards into the culture tank; a motor is arranged at the top end of the bracket; the output end of the motor is connected with a driving gear; the stirring shaft is provided with a driven gear meshed with the driving gear; a plurality of stirring rods are arranged on the stirring shaft; two scraping plates are symmetrically arranged on two sides of the stirring shaft; each scraper is connected with the stirring shaft through a connecting rod; one side of each scraper, which is far away from the stirring shaft, is contacted with the inner wall of the culture tank. According to the utility model, by arranging the motor, the driving gear, the driven gear, the stirring shaft, the stirring rod and the scraping plate, the microbial thalli and the culture solution can be fully stirred, so that the microbial thalli and the culture solution are uniformly mixed, and the thalli adhered to the inner wall of the culture tank can be scraped off by the scraping plate when the scraping plate rotates, so that waste is avoided.
Description
Technical Field
The utility model relates to the technical field of industrial production equipment, in particular to a bacterial culture device for acrylamide production.
Background
Acrylamide is a white crystal chemical substance and is a raw material for producing polyacrylamide. The acrylamide is formed by taking acrylonitrile as a raw material for hydration, and the production process successively goes through 3 development stages of sulfuric acid hydration, copper-based catalytic chemical hydration and microbial nitrile hydratase bioconversion from the 50 th century. Compared to copper-based catalysis, microbiological processes have a number of advantages: the enzyme catalytic reaction is carried out at normal temperature and normal pressure, so that the production safety is improved; the conversion rate of the acrylonitrile is close to 100 percent, and unreacted raw materials do not need to be recovered; because copper-containing catalysts are not needed, copper ions are not contained in the product, the purity is higher, and the method is particularly suitable for producing polyacrylamide with ultrahigh molecular weight; simple process, less equipment investment and high economic benefit. At present, the microbial method for producing acrylamide is widely used, microbial thalli is needed to be used as a catalyst for hydration reaction of acrylonitrile in the microbial method for producing acrylamide, and a microbial thalli culture device is needed in the microbial thalli culture process. However, the existing microorganism cell culture device has a simple structure, and can not realize the thorough mixing of the microorganism and the culture solution, so that the culture effect is poor.
Disclosure of Invention
In view of the above, the present utility model provides a cell culture apparatus for producing acrylamide, which aims to solve the problems in the prior art.
In order to solve the technical problems, the utility model adopts the following technical scheme:
a cell culture apparatus for producing acrylamide, comprising: a culture tank; a bracket is arranged at the top end of the culture tank; the stirring shaft is rotationally connected to the bracket; the stirring shaft extends downwards into the culture tank; a motor is arranged at the top end of the bracket; the output end of the motor is connected with a driving gear; the stirring shaft is provided with a driven gear meshed with the driving gear; a plurality of stirring rods are arranged on the stirring shaft; two scraping plates are symmetrically arranged on two sides of the stirring shaft; each scraping plate is connected with the stirring shaft through a connecting rod; one side of each scraper, which is far away from the stirring shaft, is contacted with the inner wall of the culture tank; a jacket is sleeved outside the culture tank; a temperature control cavity is formed between the jacket and the culture tank; a water inlet pipe is arranged at the lower end of the jacket; a water outlet pipe is arranged at the upper end of the jacket; a temperature sensor is arranged in the culture tank; a feed pipe is arranged at the top end of the culture tank; the bottom end of the culture tank is provided with a discharge pipe.
Preferably, an air inlet channel is formed in the stirring shaft; an air cavity is arranged in each stirring rod; each air cavity is communicated with the air inlet channel; the top end and the bottom end of each stirring rod are provided with aeration heads communicated with the air cavity; the top end of the stirring shaft is provided with an air inlet pipe communicated with the air inlet channel.
Preferably, an induced draft fan is connected to the air inlet end of the air inlet pipe; the air inlet pipe penetrates through the temperature control cavity.
Preferably, the air inlet pipe positioned in the temperature control cavity is spirally wound on the outer circumferential side of the culture tank.
Preferably, the discharging pipe is provided with a discharging valve.
Preferably, the top end of the culture tank is provided with an exhaust pipe; an exhaust valve is arranged on the exhaust pipe.
Preferably, a pressure sensor is arranged inside the culture tank.
Preferably, the top end of the feeding pipe is provided with a sealing cover.
Preferably, the bottom end of the culture tank is provided with a base; and a support column is arranged at the bottom end of the base.
Compared with the prior art, the utility model has the following technical effects:
1) According to the utility model, the motor drives the driving gear to rotate, the driving gear drives the driven gear to rotate, the driven gear drives the stirring shaft to rotate, and the stirring shaft drives the stirring rod and the scraping plate to rotate simultaneously, so that the microbial thalli and the culture solution are fully stirred, and are uniformly mixed, the scraping plate can play a role in further improving the stirring effect, and can scrape thalli adhered on the inner wall of the culture tank, so that waste is avoided, and meanwhile, when the culture solution in the culture tank is required to be heated or cooled, the temperature of the culture solution in the culture tank is kept in a proper range through the circulating heating medium or the circulating cooling medium through the water inlet pipe, so that the influence on the culture of the microbial thalli due to overhigh or overlow temperature is avoided.
2) According to the utility model, the air inlet pipe is used for introducing oxygen into the air inlet channel, and then the oxygen sequentially passes through the connecting channel, the air cavity and the aeration head to enter the culture tank, so that microbial thalli can be fully contacted with the oxygen, and the culture effect is improved.
3) According to the utility model, the air inlet pipe penetrates through the temperature control cavity, so that oxygen entering the culture tank through the air inlet pipe can be heated or cooled, and the stability of the temperature in the culture tank is ensured.
Drawings
FIG. 1 is a schematic diagram showing the structure of a cell culture apparatus for producing acrylamide according to the present utility model;
FIG. 2 is an enlarged partial cross-sectional view of portion A of FIG. 1;
in the figure: 1. a culture tank; 2. a bracket; 3. a stirring shaft; 4. a motor; 5. a drive gear; 6. a driven gear; 7. a stirring rod; 8. a scraper; 9. a connecting rod; 10. a jacket; 11. a temperature control cavity; 12. a water inlet pipe; 13. a water outlet pipe; 14. a temperature sensor; 15. a feed pipe; 16. a discharge pipe; 17. an air intake passage; 18. an air cavity; 19. an aeration head; 20. an air inlet pipe; 21. an induced draft fan; 22. a discharge valve; 23. an exhaust pipe; 24. an exhaust valve; 25. a pressure sensor; 26. sealing cover; 27. a base; 28. a support post; 29. and a connecting channel.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Examples
Referring to FIGS. 1 to 2, the present utility model discloses a cell culture apparatus for producing acrylamide, comprising: a culture tank 1 and a base 27; the culture tank 1 is arranged at the top end of the base 27; the bottom end of the base 27 is provided with a support column 28; a bracket 2 is arranged at the top end of the culture tank 1; the bracket 2 is rotationally connected with a stirring shaft 3; the stirring shaft 3 is coaxially arranged with the culture tank 1; the stirring shaft 3 extends downwards into the culture tank 1; the top end of the bracket 2 is provided with a motor 4; the output end of the motor 4 is connected with a driving gear 5; the stirring shaft 3 is provided with a driven gear 6 meshed with the driving gear 5; a plurality of stirring rods 7 are arranged on the stirring shaft 3; a plurality of stirring rods 7 are arranged inside the culture tank 1; two scraping plates 8 are symmetrically arranged on two sides of the stirring shaft 3; each scraper 8 is fixedly connected with the stirring shaft 3 through a connecting rod 9; one side of each scraper 8 far away from the stirring shaft 3 is contacted with the inner wall of the culture tank 1; the outer side of the culture tank 1 is sleeved with a jacket 10; a temperature control cavity 11 is formed between the jacket 10 and the culture tank 1; a water inlet pipe 12 is arranged at the lower end of the jacket 10; the upper end of the jacket 10 is provided with a water outlet pipe 13; the water inlet pipe 12 and the water outlet pipe 13 are communicated with the temperature control cavity 11; the top wall of the interior of the culture tank 1 is provided with a temperature sensor 14; a feed pipe 15 is arranged at the top end of the culture tank 1; the bottom end of the culture tank 1 is provided with a discharge pipe 16; the tapping pipe 16 extends downwardly below the base 27.
According to the technical scheme, the driving gear 5 is driven to rotate through the motor 4, the driving gear 5 drives the driven gear 6 to rotate, the driven gear 6 drives the stirring shaft 3 to rotate, the stirring shaft 3 drives the stirring rod 7 and the scraping plate 8 to simultaneously rotate, so that the microbial thalli and the culture solution are fully stirred, the microbial thalli and the culture solution are uniformly mixed, the scraping plate 8 can play a role of further improving the stirring effect, thalli adhered to the inner wall of the culture tank 1 can be scraped, waste is avoided, meanwhile, the temperature in the culture tank 1 is monitored through the temperature sensor 14, when the culture solution in the culture tank 1 needs to be heated or cooled, the temperature in the culture tank 1 is kept in a proper range through a circulating heating medium or a circulating cooling medium through the water inlet pipe 12 to the temperature control cavity 11, and the culture solution temperature in the culture tank 1 is prevented from being influenced by overhigh or overlow temperature.
In this embodiment, a connecting rod 9 is disposed at the upper end and the lower end of each scraper 8 and connected to the stirring shaft 3, so as to improve the stability of the scraper.
In the present embodiment, the inside of the stirring shaft 3 is provided with an air intake passage 17; an air cavity 18 is arranged inside each stirring rod 7; each air chamber 18 communicates with the air intake passage 17 through a connecting passage 29; the top end and the bottom end of each stirring rod 7 are provided with aeration heads 19 communicated with the air cavity 18; the top end of the stirring shaft 3 is provided with an air inlet pipe 20 communicated with the air inlet channel 17; the air inlet pipe 20 is connected with the stirring shaft 3 through a rotary joint; when the microbial culturing device is used, oxygen is introduced into the air inlet channel 17 through the air inlet pipe 20, the oxygen in the air inlet channel 17 sequentially enters the culturing tank 1 through the connecting channel 29, the air cavity 18 and the aeration head 19, and meanwhile, the microbial thallus can be fully contacted with the oxygen by matching with the rotation of the stirring rod 7, so that the culturing effect is improved.
In the present embodiment, an induced draft fan 21 is connected to the air inlet end of the air inlet pipe 20; the air inlet pipe 20 penetrates through the temperature control cavity 11, so that the air inlet pipe 20 penetrates through the temperature control cavity 11, oxygen entering the culture tank 1 through the air inlet pipe 20 can be heated or cooled, and the stability of the temperature inside the culture tank 1 is ensured.
In this embodiment, the air inlet pipe 20 located in the temperature control chamber 11 is spirally wound around the outer periphery of the culture tank 1, so that the time for oxygen to pass through the temperature control chamber can be prolonged, and the temperature of oxygen entering the culture tank is more approximate to the internal temperature of the culture tank.
In this embodiment, the discharge pipe 16 is provided with a discharge valve 22, which facilitates control of discharge.
In this embodiment, the top end of the culture tank 1 is provided with an exhaust pipe 23; the exhaust pipe 23 is provided with an exhaust valve 24; when the pressure in the culture tank 1 is too high, the exhaust valve 24 is opened, and the pressure in the culture tank 1 can be released by exhausting the air through the exhaust pipe 23.
In this embodiment, the upper wall of the interior of the culture tank 1 is provided with a pressure sensor 25 for monitoring the pressure inside the culture tank.
In this embodiment, a sealing cap 26 is provided at the top end of the feed tube 15 for sealing the feed tube 15.
In the present embodiment, water is used as the circulating heating medium or the circulating cooling medium.
In this embodiment, the induced draft fan 21 is disposed at the top end of the base 27.
The foregoing description is only of the preferred embodiments of the present utility model and is not intended to limit the technical scope of the present utility model, so that any minor modifications, equivalent changes and modifications made to the above embodiments according to the technical principles of the present utility model still fall within the scope of the technical solutions of the present utility model.
Claims (9)
1. A cell culture apparatus for producing acrylamide, comprising: a culture tank (1); a bracket (2) is arranged at the top end of the culture tank (1); a stirring shaft (3) is rotatably connected to the bracket (2); the stirring shaft (3) extends downwards into the culture tank (1); a motor (4) is arranged at the top end of the bracket (2); the output end of the motor (4) is connected with a driving gear (5); a driven gear (6) meshed with the driving gear (5) is arranged on the stirring shaft (3); a plurality of stirring rods (7) are arranged on the stirring shaft (3); two scraping plates (8) are symmetrically arranged on two sides of the stirring shaft (3); each scraper (8) is connected with the stirring shaft (3) through a connecting rod (9); one side of each scraper (8) far away from the stirring shaft (3) is contacted with the inner wall of the culture tank (1); a jacket (10) is sleeved outside the culture tank (1); a temperature control cavity (11) is formed between the jacket (10) and the culture tank (1); a water inlet pipe (12) is arranged at the lower end of the jacket (10); the upper end of the jacket (10) is provided with a water outlet pipe (13); a temperature sensor (14) is arranged inside the culture tank (1); a feed pipe (15) is arranged at the top end of the culture tank (1); the bottom end of the culture tank (1) is provided with a discharge pipe (16).
2. The cell culture apparatus for producing acrylamide according to claim 1, characterized in that an air intake passage (17) is provided inside the stirring shaft (3); an air cavity (18) is arranged in each stirring rod (7); each air cavity (18) is communicated with the air inlet channel (17); the top end and the bottom end of each stirring rod (7) are provided with aeration heads (19) communicated with the air cavity (18); an air inlet pipe (20) communicated with the air inlet channel (17) is arranged at the top end of the stirring shaft (3).
3. The cell culture apparatus for producing acrylamide according to claim 2, characterized in that an induced draft fan (21) is connected to an air inlet end of the air inlet pipe (20); the air inlet pipe (20) penetrates through the temperature control cavity (11).
4. The cell culture apparatus for producing acrylamide according to claim 3, wherein the air inlet pipe (20) located in the temperature control chamber (11) is spirally wound around the outer peripheral side of the culture tank (1).
5. The cell culture apparatus for producing acrylamide according to claim 1, characterized in that the discharge pipe (16) is provided with a discharge valve (22).
6. The cell culture apparatus for producing acrylamide according to claim 1, characterized in that an exhaust pipe (23) is provided at the top end of the culture tank (1); an exhaust valve (24) is arranged on the exhaust pipe (23).
7. The cell culture apparatus for producing acrylamide according to claim 6, wherein a pressure sensor (25) is provided inside the culture tank (1).
8. The cell culture apparatus for producing acrylamide according to claim 1, wherein a sealing cover (26) is provided at the top end of the feed pipe (15).
9. The cell culture apparatus for producing acrylamide according to claim 1, characterized in that a base (27) is provided at the bottom end of the culture tank (1); and a support column (28) is arranged at the bottom end of the base (27).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321285977.3U CN219907657U (en) | 2023-05-24 | 2023-05-24 | Thallus culture device for acrylamide production |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321285977.3U CN219907657U (en) | 2023-05-24 | 2023-05-24 | Thallus culture device for acrylamide production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219907657U true CN219907657U (en) | 2023-10-27 |
Family
ID=88423679
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321285977.3U Active CN219907657U (en) | 2023-05-24 | 2023-05-24 | Thallus culture device for acrylamide production |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219907657U (en) |
-
2023
- 2023-05-24 CN CN202321285977.3U patent/CN219907657U/en active Active
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