CN219824231U - Sorting consumable pipe group and sorting equipment - Google Patents

Abstract

The utility model provides a sorting consumable pipe group and sorting equipment, wherein the sorting consumable pipe group comprises a sample inlet for injecting a sample liquid, a fluid filter for filtering impurities, a magnetic sorting column for sorting cells marked by magnetic beads from the sample liquid through magnetic adsorption, and an output end for outputting the liquid; the set of sorting consumable items further includes a first conduit communicating between the sample inlet and the fluid filter, a second conduit communicating between the fluid filter and the magnetic sorting column, and a third conduit communicating between the magnetic sorting column and the output for mounting to a drive pump. The utility model realizes the whole group replacement of the sorting consumable pipe group, improves the replacement efficiency, avoids the waste of the consumable pipe group, and improves the cell sorting efficiency of sorting equipment.

Description

Sorting consumable pipe group and sorting equipment

Technical Field

The utility model belongs to the technical field of cell processing, and particularly relates to a sorting consumable pipe group and sorting equipment.

Background

At present, in sorting facilities, the consumptive material nest of tubes that communicates between each container all needs the operating personnel to communicate the installation respectively to each pipeline manually, still need change each pipeline one by one after every process step finishes, has to change inefficiency and leads to the problem that cell sorting facilities sorting efficiency is low. In addition, because of the difference of manual operation, the length of each pipeline to be communicated is different from person to person, and the overlong pipeline is reserved between the connecting points of each pipeline, so that the waste of consumable pipelines and the increase of the residual liquid treatment capacity in the pipelines are caused, and the technical problem of cost increase is caused.

Disclosure of Invention

The utility model provides a sorting consumable pipe group and sorting equipment aiming at the technical problems of low sorting efficiency, increased waste cost of consumable pipelines and the like of sorting equipment in the prior art.

In view of the above technical problems, embodiments of the present utility model provide a sorting consumable tube set including a sample inlet for injecting a sample liquid, a fluid filter for filtering impurities, a magnetic sorting column for sorting magnetic bead-labeled cells from the sample liquid by magnetic adsorption, and an output end for outputting the liquid;

the sorting consumable tube set further includes a first tube communicating between the sample inlet and the fluid filter, a second tube communicating between the fluid filter and the magnetic sorting column, and a third tube communicating between the magnetic sorting column and the output for mounting to a drive pump.

Optionally, the sorting consumable tube set further comprises a separation column for adsorbing a portion of cells from a sample liquid, the second tube comprises a first connection tube and a second connection tube, the first connection tube is communicated between the fluid filter and the separation column; one end of the second connecting pipe is communicated with one end of the separation column, which is far away from the first connecting pipe, and the other end of the second connecting pipe is communicated with one end of the magnetic separation column, which is far away from the third pipeline.

Optionally, the separation column is provided with a first separation space communicated between the second pipeline and the second connecting pipe, and the first separation space is accommodated with first iron beads for separating viscous cells from a sample liquid; the magnetic separation column is provided with a second separation space communicated between the third pipeline and the second connecting pipe, and second iron beads for adsorbing cells marked by the magnetic beads under the action of an external magnetic field are accommodated in the second separation space.

Optionally, the output end includes a first waste inlet and outlet in communication with the third pipeline;

the sorting consumable pipe group further comprises a first communication pipeline, a second communication pipeline, a third communication pipeline, a fourth communication pipeline, a cleaning inlet for injecting cleaning liquid and a fourth pipeline communicated between the cleaning inlet and the second pipeline;

the first communication pipeline is communicated between the fourth pipeline and the first pipeline; one end of the second communication pipeline is communicated with the fourth pipeline and is positioned between the first connecting point and the second connecting point, and the other end of the second communication pipeline is communicated with the second pipeline; the first connecting point is a connecting point of the first communication pipeline and the fourth pipeline; the second connection point is a connection point of the fourth pipeline and the second pipeline;

one end of the third communication pipeline is communicated with the fourth pipeline and is positioned between the first connecting point and the second connecting point, and the other end of the third communication pipeline is communicated with the third pipeline; one end of the fourth communication pipeline is communicated with the third pipeline and is positioned between the magnetic separation column and a third connecting point, and the other end of the fourth communication pipeline is communicated with the second pipeline and is positioned between the fluid filter and a fourth connecting point; the third connection point is a connection point of the third communication pipeline and the third pipeline; the fourth connection point is a connection point of the second communication pipeline and the second pipeline.

Optionally, the sorting consumable tube set further comprises a sterile filter, a fifth pipeline communicated with the sterile filter, and a fifth communication pipeline communicated between the first pipeline and the fifth pipeline;

one end of the fifth pipeline far away from the sterile filter is communicated with the second pipeline and is positioned between the fluid filter and a fifth connecting point; the fifth connection point is a connection point of the fourth communication pipeline and the second pipeline.

Optionally, the sorting consumable tube set further comprises a sixth pipeline and a second waste liquid inlet and outlet communicated with the sixth pipeline; and one end of the sixth pipeline, which is far away from the second waste liquid inlet and outlet, is communicated with the fifth pipeline.

Optionally, the output end comprises a female selection outlet for outputting female selection liquid and communicating with the third pipeline, and a male selection outlet for outputting male selection liquid and communicating with the third pipeline.

Optionally, the sorting consumable tube set further comprises a filter drip chamber arranged on the second pipeline and used for filtering bubbles in the liquid.

The utility model also provides sorting equipment which comprises a driving pump and the sorting consumable pipe group, wherein the third pipeline is arranged on the driving pump.

Optionally, the sorting consumable tube group comprises a magnetic sorting column provided with a second sorting space; the sorting apparatus further comprises a magnetic field generating means arranged outside the second sorting space for generating an external magnetic field, and a position moving means for controlling a change in distance between the magnetic field generating means and the magnetic sorting column.

Optionally, the sorting device further comprises a pressure sensor arranged on the third pipeline, a first bubble sensor arranged on the first pipeline, a second bubble sensor arranged on the second pipeline and a third bubble sensor arranged on the third pipeline.

The sorting consumable tube group comprises a sample inlet, a first pipeline, a fluid filter, a second pipeline, a magnetic sorting column, a third pipeline and an output end which are sequentially communicated, wherein the sorting consumable tube group is a group of integral pipelines, so that when sorting equipment needs to replace the sorting consumable tube group in a sorting process (such as sorting cell liquid), the sorting equipment does not need to replace each pipeline one by one after each process step is finished, and the pipelines are not required to be manually communicated and installed by an operator, but only the whole group of sorting consumable tube groups are directly replaced, so that the replacement efficiency is improved, and the sorting efficiency of the sorting equipment is also improved; and avoid the pipeline personnel who causes when artifical pipe reservation overlength pipeline to wear, avoided sorting the waste of consumptive material nest of tubes, also reduced the cost waste that causes because of remaining liquid treatment in the overlength pipeline.

Drawings

The utility model will be further described with reference to the drawings and examples.

Fig. 1 is a schematic structural view of a sorting apparatus according to an embodiment of the present utility model.

Fig. 2 is a schematic structural view of a sorting apparatus according to another embodiment of the present utility model.

Reference numerals in the specification are as follows:

100. sorting consumable pipe groups; 111. a sample inlet; 112. a purge inlet; 113. a second waste inlet and outlet; 120. a fluid filter; 131. a separation column; 132. a magnetic separation column; 140. an output end; 141. a first waste inlet and outlet; 142. a female selection outlet; 143. a cation separation outlet; 151. a first pipeline; 152. a second pipeline; 1521. a first connection pipe; 1522. a second connection pipe; 153. a third pipeline; 1531. a first pipe section; 1532. a peristaltic tube; 1533. a second pipe section; 154. a fourth pipeline; 155. a fifth pipeline; 156. a sixth pipeline; 161. a first communication line; 162. a second communication line; 163. a third communication line; 164. a fourth communication line; 165. a fifth communication line; 180. a sterile filter; 190. filtering the dropping funnel; 200. driving a pump; 300. a magnetic field generating device; 410. a pressure sensor; 420. a second pressure sensor; 510. a first bubble sensor; 520. a second bubble sensor; 530. a third bubble sensor; 540. a fourth bubble sensor; 550. a fifth bubble sensor; 610. a first waste liquid container; 620. a second waste liquid container; 630. a female sorting container; 640. a male selection container; 701. a first pipe clamp; 702. a second pipe clamp; 703. a third tube clamp; 704. a fourth tube clamp; 705. a fifth pipe clamp; 706. a sixth tube clamp; 707. a seventh tube clamp; 801. a first switching valve; 802. a second switching valve; 803. a third switching valve; 804. a fourth switching valve; 805. a fifth switching valve; 806. a sixth switching valve; 807. a seventh switching valve; 808. an eighth switching valve; 809. a ninth switching valve; 810. a tenth switching valve; 811. an eleventh switching valve; 812. a twelfth switching valve; 813. a thirteenth switching valve; 814. a fourteenth switching valve; 815. fifteenth switching valve.

Detailed Description

In order to make the technical problems, technical schemes and beneficial effects solved by the utility model more clear, the utility model is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the utility model.

It is to be understood that the directions or positional relationships indicated by the terms "upper", "lower", "left", "right", "front", "rear", "middle", etc., are based on the directions or positional relationships shown in the drawings, are merely for convenience of description and simplification of the description, and do not indicate or imply that the apparatus or element in question must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as limiting the utility model.

As shown in fig. 1, an embodiment of the present utility model provides a sorting consumable tube set 100 including a sample inlet 111 for injecting a sample liquid, a fluid filter 120 for filtering impurities, a magnetic sorting column 132 for magnetically sorting magnetic bead-labeled cells (including immunomagnetic bead cells and magnetic bead-labeled bacteria, etc.), and an output 140 for outputting a liquid; the set of sorting consumable supplies 100 further comprises a first conduit 151 communicating between the sample inlet 111 and the fluid filter 120, a second conduit 152 communicating between the fluid filter 120 and the magnetic sorting column 132, and a third conduit 153 communicating between the magnetic sorting column 132 and the output 140 for mounting to a drive pump 200.

In this embodiment, the sample solution contains cells marked by magnetic beads (including immunomagnetic bead cells and bacteria marked by magnetic beads, etc.), the sample inlet 111 may be in communication with a sample solution container (not shown), the sample solution in the sample solution container is driven by the driving pump 200 to flow through the first pipeline 151 via the sample inlet 111, then to filter impurities in the sample solution (such as blood clots, micro polymers and micro particle fragments in blood, and blood clots and micro particle fragments in salvaged blood products) via the fluid filter 120, and then to enter the magnetic separation column 132 via the second pipeline 152, after the magnetic separation column 132 performs magnetic separation on the cells marked by magnetic beads in the sample solution (i.e. the cells marked by magnetic beads are magnetically adsorbed in the magnetic separation column 132), finally, the remaining liquid such as the separated negative cells is output to the consumable tube set 100 after flowing through the output end 140 via the third pipeline 153. It will be appreciated that the sample inlet 111 and the first conduit 151 may be a single integral conduit of the same or different bore; the sample inlet 111 may also be a separate connector (e.g., luer connector) in communication with the sample fluid container and the first conduit 151, respectively.

The sorting consumable tube group 100 provided by the utility model comprises a sample inlet 111, a first pipeline 151, a fluid filter 120, a second pipeline 152, a magnetic sorting column 132, a third pipeline 153 and an output end 140 which are sequentially communicated, wherein the sorting consumable tube group 100 is a group of integral pipelines, so that when sorting equipment needs to replace the sorting consumable tube group in the sorting process, each pipeline does not need to be replaced one by one after each process step is finished, each pipeline does not need to be communicated and installed manually by an operator, but the whole group of sorting consumable tube group 100 is replaced directly, the replacement efficiency is improved, and the sorting efficiency of the sorting equipment is also improved; and avoid the pipeline personnel who causes when artifical pipe reservation overlength pipeline to wear, avoided sorting the waste of consumptive material nest of tubes, also reduced the cost waste that causes because of remaining liquid treatment in the overlength pipeline.

As shown in fig. 1, the present utility model also provides a sorting apparatus including a driving pump 200 and the sorting consumable pipe group 100. In this embodiment, the sample fluid may be continuously input into the sorting consumable tubing set 100 through the sample inlet 111 and the fluid processed by the sorting consumable tubing set 100 may be output through the output port 140 under the driving of the driving pump 200. In this process, after the impurities in the sample fluid (such as blood clots, micro polymers and micro particle fragments in blood, blood clots and micro particle fragments in salvaged blood products, etc.) are filtered out by the fluid filter 120, the magnetic bead labeled cells are sorted by the magnetic sorting column 132, so that not only can the magnetic bead labeled cells be successfully sorted, but also the impurities in the sample fluid are prevented from blocking the gaps between the second iron beads in the magnetic sorting column 132 (blocking can cause the reduction of the sorting efficiency of the magnetic bead labeled cells), thereby improving the efficiency and the precision of cell sorting.

In some embodiments, the drive pump 200 includes, but is not limited to, a peristaltic pump that may not contact the liquid while driving the liquid flow so as not to contaminate the liquid. It will be appreciated that the drive pump 200 may be other types of liquid pumps as long as the effect of driving the flow of liquid is achieved.

As shown in fig. 2, in some embodiments, the sorting consumable tube set further includes a separation column 131 for adsorbing a portion of cells from the sample fluid (where a portion of cells refers to a portion of components of the sample fluid that need to be captured and removed by the separation column that need not be present in the resulting cationic liquid, including, but not limited to, viscous cells such as monocytes, granulocytes and platelets present in the cell suspension during separation of blood cells, and components that may be non-specifically bound to the magnetic separation column and thus need to be prefiltered by the separation column, etc.); the second pipe 152 includes a first connection pipe 1521 and a second connection pipe 1522, the first connection pipe 1521 being communicated between the fluid filter 120 and the separation column 131; one end of the second connecting pipe 1522 is connected to one end of the separation column 131 away from the first connecting pipe 1521, and the other end of the second connecting pipe 1522 is connected to one end of the magnetic separation column 132 away from the third pipeline 153.

In this embodiment, the fluid filter 120, the separation column 131 and the magnetic separation column 132 are simultaneously disposed in the sorting consumable tube group, so that three-stage sorting can be performed on the sample fluid, specifically, impurities (such as blood clots, micro-polymers and micro-particle fragments in blood, blood clots and micro-particle fragments in salvaged blood products, etc.) in the sample fluid are filtered out in the fluid filter 120, and the remaining liquid is output to the separation column 131, so that the above-mentioned partial cells (the above-mentioned partial cells are separated by the first iron beads and remain in the first sorting space of the separation column 131) are separated by the separation column 131, the remaining residual liquid (including the cells marked by the magnetic beads and negative cells, etc.) is output to the magnetic separation column 132, and then the cells marked by the magnetic beads therein are magnetically adsorbed by the magnetic separation column 132, that is, the cells marked by the magnetic beads are adsorbed on the second iron beads having magnetism due to the action of the external magnetic field, and the remaining negative separation liquid flows out from the output end 140, so that the impurities in the sample fluid are separated by the separation column 131 (the above-mentioned three-stage separation) are avoided, the accuracy of the magnetic separation column is improved, such as the magnetic separation efficiency of the marked by the magnetic beads is improved.

In some embodiments, the separation column 131 is provided with a first separation space communicated between the second pipeline 152 and the second connecting pipe 1522, wherein the first separation space accommodates therein a first iron bead for separating viscous cells (including, in particular, the above-mentioned part of cells, including but not limited to, viscous cells, etc.) from the sample liquid; the magnetic separation column 132 is provided with a second separation space communicated between the third pipeline 153 and the second connection pipe 1522, and second iron beads for adsorbing cells marked by magnetic beads under the action of an external magnetic field are accommodated in the second separation space. The three-dimensional structure of the separation column is basically the same as that of the magnetic separation column, but the sizes of the separation column and the magnetic separation column, the diameters of the first iron beads and the second iron beads and the like can be set according to requirements, for example, the sizes of the first separation space and the second separation space of the separation column and the magnetic separation column can be the same or different, and the diameters of the first iron beads and the second iron beads can be the same or different, so that holes or channels with different sizes and numbers from those in the magnetic separation column can be provided in the separation column. In addition, the first iron beads in the separation column and the second iron beads in the magnetic separation column are the same in material, so that components which can be non-specifically bound to the second iron beads in the magnetic separation column in the separation process are captured by the separation column first, the possibility of scaling of the magnetic separation column in the separation process is reduced through the separation column, and the existence of unnecessary cells or fluid components in the finally obtained cationic liquid is finally reduced through the arrangement, so that the separation precision of the separation equipment is improved.

In one embodiment, the first and second beads are the same material and the second beads in the second separation space have a diameter greater than or equal to the diameter of the first beads in the first separation space, such that the pore size between the first beads in the separation column will be smaller than the pore size between the second beads, such that the pore size of the permeable matrix can be selected to remove large tumor cells from the sample prior to the fluid passing through the magnetic separation column. Thus, after a portion of the cells (including but not limited to, the tacky cells such as monocytes, granulocytes and platelets present during the blood cell separation process, and the components that may be non-specifically bound to the magnetic separation column and thus need to be prefiltered by the separation column, e.g., large tumor cells) are separated, the remaining liquid flows out of the gaps between the first beads, and if the cells labeled with the magnetic beads are magnetically adsorbed by the second beads in the second separation space, the remaining liquid (the negative separation liquid) can smoothly flow out of the gaps between the second beads (if the diameter of the second beads is smaller than that of the first beads, there may be a portion of the negative separation liquid blocked by the second beads and unable to flow out, and the negative cells in the negative separation liquid blocked between the second beads will be washed into the positive separation liquid, thereby affecting the separation accuracy of the magnetic separation column).

In this embodiment, the cells in the sample liquid labeled with the magnetic beads are magnetically labeled, so as to be combined with the magnetic substance, and further have magnetism; i.e., the cells labeled with the magnetic beads are magnetized, thereby generating cells labeled with the magnetic beads (including immunomagnetic bead cells, bacteria labeled with the magnetic beads, etc.). The sample solution further includes the above-mentioned part of cells (wherein, part of cells refers to the part of components in the sample solution that need to be trapped and removed by the separation column and that need not appear in the final cationic liquid, including but not limited to, the viscous cells such as monocytes, granulocytes and platelets that exist in the cell suspension and the components that may be non-specifically bound to the magnetic separation column and need to be prefiltered by the separation column, etc.), so the first separation space in the separation column 131 accommodates the first iron beads, the sample solution is separated and stays in the first separation space while passing through the separation column 131, and other liquids (including the magnetic bead labeled cells) except the part of cells continue to flow to the magnetic separation column 132 under the driving of the driving pump 200.

As shown in fig. 2, in some embodiments, the sorting consumable tube set 100 of the sorting apparatus includes a magnetic sorting column 132 provided with a second sorting space; the sorting apparatus further comprises a magnetic field generating means 300 arranged outside the second sorting space for generating an external magnetic field, and a position moving means for controlling the change of the distance between the magnetic field generating means 300 and the magnetic sorting column 132.

In this embodiment, the magnetic field generating device 300 may be a magnet or other devices that generate magnetic force through electromagnetic effect, and the moving device may switch the external magnetic field or adjust the magnitude of the magnetic force of the external magnetic field in the second separation space by controlling the change of the distance between the magnetic field generating device 300 and the magnetic separation column 132.

As shown in fig. 2, in some embodiments, the output 140 includes a first waste inlet 141 in communication with the third conduit 153; the sorting consumable substance tube set 100 further includes a first communication tube 161, a second communication tube 162, a third communication tube 163, a fourth communication tube 164, a washing inlet 112 for injecting a washing liquid, and a fourth tube 154 communicating between the washing inlet 112 and the second tube 152; the first communication line 161 communicates between the fourth line 154 and the first line 151; one end of the second communication pipe 162 communicates with the fourth pipe 154 and is located between the first connection point and the second connection point, and the other end of the second communication pipe 162 communicates with the second pipe 152 (specifically, communicates with a second connection pipe 1522 in the second pipe 152); the first connection point refers to a connection point of the first communication line 161 and the fourth communication line 154; the second connection point is a connection point between the fourth pipeline 154 and the second pipeline 152; one end of the third communication pipe 163 is connected to the fourth pipe 154 and located between the first connection point and the second connection point, and the other end of the third communication pipe 163 is connected to the third pipe 153; one end of the fourth communication line 164 communicates with the third line 153 and is located between the magnetic separation column 132 and a third connection point, and the other end of the fourth communication line 164 communicates with the second line 152 and is located between the fluid filter 120 and a fourth connection point (specifically, between the separation column 131 and a fourth connection point); the third connection point is a connection point between the third communication line 163 and the third line 153; the fourth connection point refers to a connection point of the second communication line 162 and the second line 152 (specifically, a second connection pipe 1522 in the second line 152).

In this embodiment, the sorting consumable pipe set 100 is a set of integral pipes. The first waste inlet and outlet 141 may communicate with the first waste container 610, and the washing inlet 112 may communicate with a washing liquid container (not shown). The cleaning solution is injected into the fourth pipeline 154 through the cleaning inlet 112, and the fourth pipeline 154 is communicated with the second pipeline 152, so that the cleaning solution can clean part of the second pipeline 152 (including components and pipelines between the second connection point and the magnetic separation column 132), the magnetic separation column 132 and the third pipeline 153 and enter the first waste liquid container 610 through the first waste liquid inlet 141, or when air (such as first use or liquid is emptied) exists in the separation consumable pipe group 100, the separation consumable pipe group 100 is filled with the cleaning solution, so that the air is prevented from entering the separation column 131 and the magnetic separation column 132, and the separation effect is affected. The first communication line 161 is communicated between the fourth line 154 and the first line 151, so that the cleaning liquid can flow through the first line 151 through the first communication line 161 and clean or exhaust the first line 151 and the fluid filter 120. The second communication line 162 and the third communication line 163 may communicate with each other at the same location as the fourth line 154, and the second communication line 162 and the third communication line 163 may also communicate with each other at different locations as the fourth line 154. It is to be understood that the first waste inlet 141 and the third pipeline 153 may be an integral pipeline with the same or different caliber; the first waste inlet/outlet 141 may be a separate connector (e.g., luer connector) that communicates with the first waste container 610 and the third line 153, respectively. The purge inlet 112 and the fourth conduit 154 may be one integral conduit of the same or different caliber; the purge inlet 112 may also be a separate fitting (e.g., luer fitting) in communication with the purge fluid reservoir and the fourth conduit 154, respectively.

As shown in fig. 2, in some embodiments, a first pipe clamp 701 and a first switching valve 801 for controlling the on/off of the first pipe 151 are provided on the first pipe 151, and the first pipe clamp 701 and the first switching valve 801 are located between a connection point of the first pipe 161 and the first pipe 151 and the sample inlet 111. It will be appreciated that the first pipe clamp 701 and the first switching valve 801 can control the on/off of the first pipe 151, and simultaneously prevent the sample fluid in the sample fluid container from flowing into the first pipe 151 under the action of external force (such as gravity or squeezing, etc.) when the driving pump 200 is stopped (i.e., when the sample fluid is not required to be input). In some embodiments, the fourth pipe 154 is provided with a second pipe clamp 702 and a second switching valve 802 for controlling the on-off of the fourth pipe 154, and a third switching valve 803, where the second pipe clamp 702 and the second switching valve 802 are located between the first connection point and the cleaning inlet 112, and the third switching valve 803 is located between the connection point of the fourth pipe 154 and the second communication pipe 162 and the second connection point. The second pipeline 152 is provided with a fourth switch valve 804 for controlling the on-off of the second pipeline 152, and the fourth switch valve 804 is located between the second connection point and the fluid filter 120. The second connection pipe 1522 is provided with a fifth switch valve 805 for controlling the on-off of the second connection pipe 1522, and the fifth switch valve 805 is located between the fourth connection point and the separation column 131. The first waste liquid inlet and outlet 141 is provided with a third pipe clamp 703 and a sixth switching valve 806 for controlling the on-off of the first waste liquid inlet and outlet 141. The first communication pipeline 161 is provided with a seventh switch valve 807 for controlling the on-off of the first communication pipeline 161. The second communication pipeline 162 is provided with an eighth switch valve 808 for controlling the on-off of the second communication pipeline 162. The third communication pipeline 163 is provided with a ninth switch valve 809 for controlling the on-off of the third communication pipeline 163. The fourth communication pipeline 164 is provided with a tenth switch valve 810 for controlling the on-off of the fourth communication pipeline 164. It will be appreciated that the above-described tube clamps and on-off valves may be individually controlled on-off as desired to control the different flow directions of the fluids in the sorting consumable tubing set 100.

In some embodiments, the third pipeline 153 further comprises a first pipe section 1531, a peristaltic pipe 1532 and a second pipe section 1533 which are sequentially communicated, the peristaltic pipe 1532 is mounted on the driving pump 200, one end of the first pipe section 1531 away from the peristaltic pipe 1532 is communicated with the magnetic separation column 132, and one end of the second pipe section 1533 away from the peristaltic pipe 1532 is communicated with the output end 140.

In the above embodiment, the peristaltic tube 1532 is made of a soft material that is matched to the exact control of the driven pump 200, so that the driving pump 200 can control the liquid driven by the peristaltic tube more accurately by mounting the peristaltic tube to the driving pump 200.

As shown in fig. 2, in some embodiments, the sorting consumable tubing set 100 further includes a sterile filter 180, a fifth tubing 155 in communication with the sterile filter 180, and a fifth communication tubing 165 in communication between the first tubing 151 and the fifth tubing 155; an end of the fifth line 155 remote from the sterile filter 180 communicates with the second line 152 and is located between the fluid filter 120 and a fifth connection point (in particular, between the separation column 131 and the fifth connection point); the fifth connection point refers to a connection point of the fourth communication line 164 and the second line 152 (specifically, the second connection pipe 1522 in the second line 152).

In this embodiment, the sorting consumable pipe set 100 is a set of integral pipes. The fifth line 155 communicates with the air through the sterile filter 180 so that air pressure can be balanced when needed by entering air into the sorting consumable tubing set 100. The fifth pipe 155 is connected to the second connection pipe 1522, so that air can enter the second connection pipe 1522 of the second pipe 152, and thus, all the liquid in the interconnected pipes between the connection point of the fifth pipe 155 and the second connection pipe 1522 and the waste liquid inlet and outlet can be emptied. And since the fifth communication line 165 is communicated between the first line 151 and the fifth line 155, after air enters the first line 151, it is possible to drain all the mutually communicated lines between the connection point of the first line 151 and the fifth communication line 165 to the waste liquid inlet and outlet. It is understood that the fifth line 155 and the second communication line 162 may communicate with each other at the same location or at different locations with the second connection pipe 1522.

As shown in fig. 2, in some embodiments, the fifth pipeline 155 is provided with a fourth pipe clamp 704, an eleventh switching valve 811, and a twelfth switching valve 812; the fourth pipe clamp 704 and the eleventh switching valve 811 are located between the connection point of the fifth communication pipe 165 and the fifth pipe 155 and the aseptic filter 180, and the twelfth switching valve 812 is located between the connection point of the fifth communication pipe 165 and the fifth pipe 155 and the fourth connection point. It will be appreciated that the above-described tube clamps and on-off valves may be individually controlled on-off as desired to control the different flow directions of the liquid or gas in the sorting consumable tube set 100.

As shown in fig. 2, in some embodiments, the sorting consumable tubing set 100 further includes a sixth tubing 156 and a second waste inlet 113 in communication with the sixth tubing 156; the end of the sixth pipeline 156 away from the second waste liquid inlet/outlet 113 is communicated with the fifth pipeline 155.

In this embodiment, the sorting consumable pipe set 100 is a set of integral pipes. The second waste liquid inlet and outlet 113 may be in communication with the second waste liquid container 620, and the waste liquid in the sorting consumable tube set 100 is input into the second waste liquid container 620 through the second waste liquid inlet and outlet 113, and the waste liquid in the second waste liquid container 620 may also be input into the sorting consumable tube set 100 through the second waste liquid inlet and outlet 113. It will be appreciated that the second waste inlet and the sixth conduit 156 may be an integral conduit of the same or different caliber; the second waste inlet may also be a separate fitting (e.g., luer fitting) in communication with the second waste container 620 and the sixth conduit 156, respectively. The sixth conduit 156 and the fifth communication conduit 165 may communicate with each other at the same location or at different locations as the fifth conduit 155. It will be appreciated that in one embodiment of the utility model, the washing of the magnetic bead labeled cells adsorbed on the magnetic separation column 132 may be performed by feeding a certain amount of waste liquid through the second waste liquid container 620.

In some embodiments, as shown in fig. 2, a fifth clamp 705 and a thirteenth on-off valve 813 are provided on the sixth conduit 156. It will be appreciated that the above-described tube clamps and on-off valves may be individually controlled on-off as desired to control the different flow directions of the liquid or gas in the sorting consumable tube set 100.

As shown in fig. 2, in some embodiments, the output 140 includes a female selection outlet 142 for outputting a female selection liquid and communicating with the third conduit 153, and a male selection outlet 143 for outputting a male selection liquid (a liquid containing magnetic bead labeled cells magnetically sorted by the magnetic sorting column 132) and communicating with the third conduit 153.

In this embodiment, the sorting consumable pipe set 100 is a set of integral pipes. The female sorting outlet 142 may be in communication with the female sorting container 630 and the male sorting outlet 143 may be in communication with the male sorting container 640. The magnetic bead labeled cells (including immunomagnetic bead cells and magnetic bead labeled bacteria) in the sample liquid are adsorbed on the separation column 131 and the magnetic separation column 132, and the other substances (i.e., the negative separation liquid) except the magnetic bead labeled cells are continuously flowed under the driving of the driving pump 200 and are inputted into the negative separation container 630 through the negative separation outlet 142. After the sample liquid is pumped out or the input of the sample liquid is actively interrupted, the separation column 131 and the magnetic separation column 132 may be demagnetized (that is, the separation column 131 and the magnetic separation column 132 are magnetically eliminated and no more magnetic adsorption is performed on the cells marked by the magnetic beads), and then the washing liquid may be input through the washing liquid container or the waste liquid may be input through the second waste liquid container 620, so that the cells marked by the magnetic beads, which are previously adsorbed on the separation column 131 and the magnetic separation column 132, may be washed (the mixture of the cells marked by the magnetic beads, the washing liquid, the waste liquid, and the like is the cation separation liquid) and input into the cation separation container 640 through the cation separation outlet 143. It will be appreciated that the female outlet 142, the male outlet 143 and the first waste inlet 141 may be in communication with the third conduit 153 at the same location, and that the female outlet 142, the male outlet 143 and the first waste inlet 141 may also be in communication with the third conduit 153 at different locations.

In some embodiments, as shown in fig. 2, a sixth conduit clip 706 and a fourteenth on-off valve 814 are provided on the female outlet 142. A seventh pipe clamp 707 and a fifteenth on-off valve 815 are provided on the male outlet 143. It will be appreciated that the above-described pipe clamp and on-off valve may be separately controlled as desired to control the different outlet openings of the output liquid in the output 140.

As shown in fig. 2, in some embodiments, the set of sorting consumable tubing 100 further includes a filter drip funnel 190 disposed on the second tubing 152 for filtering bubbles in the liquid entering the magnetic sorting column 132. In this embodiment, the filter dropping funnel 190 is used to remove impurities such as bubbles that may exist. The filter drip funnel 190 is connected in series with the fluid filter 120 to filter the impurities such as coagulated mass and air in the flowing liquid.

As shown in fig. 2, in some embodiments, the sorting apparatus further includes a pressure sensor 410 disposed on the third line 153, a first bubble sensor 510 disposed on the first line 151, a second bubble sensor 520 disposed on the second line 152, and a third bubble sensor 530 disposed on the third line 153. Specifically, a pressure sensor 410 is located between the drive pump 200 and the magnetic sorting column 132; a third bubble sensor 530 is located between the drive pump 200 and the magnetic sorting column 132; as shown in fig. 2, the sorting apparatus further includes a fourth bubble sensor 540 disposed on the second connection pipe 1522.

In the present embodiment, the pressure sensor 410 is configured to detect a pressure value of the liquid flowing through the driving pump 200 through the third pipe 153, so that the driving power of the driving pump 200 is adjusted according to the detected pressure value. The first bubble sensor 510 is used to detect the state of the flow of the liquid flowing through the first pipe 151, that is, the first bubble sensor 510 may be used to detect whether the liquid in the sample liquid container/cleaning liquid container/second waste liquid container 620 is drained and the flow direction of the liquid, thereby determining whether the related operation step of driving the liquid flow by the driving pump 200 has been completed, and further controlling the operation of the driving pump 200 or the switching of each switching valve to perform the subsequent steps. The second bubble sensor 520 is configured to detect a state of the flow of the liquid flowing through the second pipe 152, the third bubble sensor 530 is configured to detect a state of the flow of the liquid flowing through the third pipe 153, and the fourth bubble sensor 540 is configured to detect a state of the flow of the liquid flowing through the second connection pipe 1522. That is, the second bubble sensor 520 is used to detect whether there is a flow of liquid in the second pipeline 152 and a flow direction of the liquid, and the third bubble sensor 530 and the fourth bubble sensor 540 may be used to detect whether there is a flow of liquid in the magnetic separation column 132 and a flow direction of the liquid together, thereby determining whether the operation steps related to the driving of the liquid by the driving pump 200 have been completed, and further controlling the operation of the driving pump 200 or the switching of each switching valve to perform the subsequent steps.

As shown in fig. 2, in some embodiments, the sorting apparatus further includes a second pressure sensor 420 disposed on the second communication line 162 and a fifth bubble sensor 550 disposed on the fifth line 155.

In this embodiment, the second pressure sensor 420 is configured to detect a pressure value of the liquid passing through the second communication line 162, so as to adjust the driving power of the driving pump 200 according to the detected pressure value. The fifth bubble sensor 550 is used to detect the state of the flow of the liquid flowing through the fifth pipe 155, that is, the fifth bubble sensor 550 may be used to detect whether the liquid in the fifth pipe 155 runs out and the flow direction of the liquid, thereby determining whether the related operation step of the driving pump 200 to drive the flow of the liquid has been completed, and further controlling the operation of the driving pump 200 or the switching of each switching valve to perform the subsequent steps.

In some embodiments, the pipes may be in communication with each other via an adapter. In other embodiments, the pipes may be connected to each other by bonding or integrally forming, etc., and it is understood that the adaptor includes, but is not limited to, a three-way joint or a four-way joint, and different access ends of the adaptor may be set to different sizes of calibers, so as to access pipes with different sizes of calibers, and the adaptor may also be connected to pipes with different degrees of hardness, so as to connect pipes with different degrees of hardness and/or different sizes of calibers. It can be appreciated that in the above embodiments of the present utility model, a part of the pipelines in the sorting consumable pipe group are shared, so that the utilization rate of the sorting consumable pipe group is improved.

The above embodiments of the sorting consumable tube set and the sorting device of the present utility model are only examples, and are not intended to limit the present utility model, and any modifications, equivalent substitutions and improvements made within the spirit and principles of the present utility model should be included in the scope of the present utility model.

Claims (11)

1. A sorting consumable tube set, characterized by comprising a sample inlet for injecting a sample liquid, a fluid filter for filtering impurities, a magnetic sorting column for sorting cells marked by magnetic beads from the sample liquid by magnetic adsorption, and an output end for outputting the liquid;

the sorting consumable tube set further includes a first tube communicating between the sample inlet and the fluid filter, a second tube communicating between the fluid filter and the magnetic sorting column, and a third tube communicating between the magnetic sorting column and the output for mounting to a drive pump.

2. The set of sorting consumable supplies according to claim 1, further comprising a separation column for adsorbing a portion of the cells from the sample fluid, the second line comprising a first connection tube and a second connection tube, the first connection tube being in communication between the fluid filter and the separation column; one end of the second connecting pipe is communicated with one end of the separation column, which is far away from the first connecting pipe, and the other end of the second connecting pipe is communicated with one end of the magnetic separation column, which is far away from the third pipeline.

3. The set of sorting consumable supplies according to claim 2, characterized in that the separation column is provided with a first sorting space communicating between the second conduit and the second connecting tube, the first sorting space accommodating therein first iron beads for separating viscous cells from a sample liquid; the magnetic separation column is provided with a second separation space communicated between the third pipeline and the second connecting pipe, and second iron beads for adsorbing cells marked by the magnetic beads under the action of an external magnetic field are accommodated in the second separation space.

4. The set of sorting consumable supplies according to claim 1 or 2, characterized in that the output comprises a first waste inlet and outlet communicating with the third line;

the sorting consumable pipe group further comprises a first communication pipeline, a second communication pipeline, a third communication pipeline, a fourth communication pipeline, a cleaning inlet for injecting cleaning liquid and a fourth pipeline communicated between the cleaning inlet and the second pipeline;

the first communication pipeline is communicated between the fourth pipeline and the first pipeline; one end of the second communication pipeline is communicated with the fourth pipeline and is positioned between the first connecting point and the second connecting point, and the other end of the second communication pipeline is communicated with the second pipeline; the first connecting point is a connecting point of the first communication pipeline and the fourth pipeline; the second connection point is a connection point of the fourth pipeline and the second pipeline;

one end of the third communication pipeline is communicated with the fourth pipeline and is positioned between the first connecting point and the second connecting point, and the other end of the third communication pipeline is communicated with the third pipeline; one end of the fourth communication pipeline is communicated with the third pipeline and is positioned between the magnetic separation column and a third connecting point, and the other end of the fourth communication pipeline is communicated with the second pipeline and is positioned between the fluid filter and a fourth connecting point; the third connection point is a connection point of the third communication pipeline and the third pipeline; the fourth connection point is a connection point of the second communication pipeline and the second pipeline.

5. The sorting consumable tubing set of claim 4, further comprising a sterile filter, a fifth line communicating with the sterile filter, and a fifth communication line communicating between the first line and the fifth line;

one end of the fifth pipeline far away from the sterile filter is communicated with the second pipeline and is positioned between the fluid filter and a fifth connecting point; the fifth connection point is a connection point of the fourth communication pipeline and the second pipeline.

6. The sorting consumable tube set of claim 5, further comprising a sixth conduit and a second waste inlet and outlet in communication with the sixth conduit; and one end of the sixth pipeline, which is far away from the second waste liquid inlet and outlet, is communicated with the fifth pipeline.

7. The set of sorting consumable supplies according to claim 1 or 2, characterized in that the output end comprises a female outlet for outputting a female liquid and communicating with the third line, and a male outlet for outputting a male liquid and communicating with the third line.

8. The set of sorting consumable supplies according to claim 1 or 2, characterized in that the set of sorting consumable supplies further comprises a filter drip chamber arranged on the second line for filtering bubbles in a liquid.

9. Sorting apparatus comprising a drive pump and a set of sorting consumable items according to any one of claims 1 to 8, the third line being mounted on the drive pump.

10. The sorting apparatus of claim 9, wherein the set of sorting consumable items comprises a magnetic sorting column provided with a second sorting space; the sorting apparatus further comprises a magnetic field generating means arranged outside the second sorting space for generating an external magnetic field, and a position moving means for controlling a change in distance between the magnetic field generating means and the magnetic sorting column.

11. The sorting apparatus of claim 9, further comprising a pressure sensor disposed on the third conduit, a first bubble sensor disposed on the first conduit, a second bubble sensor disposed on the second conduit, and a third bubble sensor disposed on the third conduit.