CN219050408U

CN219050408U - Protein purification system

Info

Publication number: CN219050408U
Application number: CN202223445760.2U
Authority: CN
Inventors: 潘文煌; 王龙刚; 唐思远; 王影; 沈克强; 陈智胜
Original assignee: Wuxi Yaoming Biotechnology Co ltd
Current assignee: Wuxi Yaoming Biotechnology Co ltd
Priority date: 2022-12-22
Filing date: 2022-12-22
Publication date: 2023-05-23
Anticipated expiration: 2032-12-22

Abstract

The utility model relates to the technical field of biological purification, and discloses a protein purification system. Wherein the protein purification system comprises a first buffer tank, a peristaltic pump, a plunger pump, a filter, a first branch, a first multi-way valve, an output pipeline and a collection tank. The first buffer tank stores liquid to be processed; the inlet of the peristaltic pump is connected with the outlet of the first buffer tank; the filter is used for filtering the liquid to be processed; the first branch comprises a second buffer tank and a plunger pump, and an inlet of the plunger pump is connected with an outlet of the second buffer tank; the first multi-way valve is respectively connected with the outlet of the plunger pump, the outlet of the peristaltic pump and the inlet of the filter; one end of the output pipeline is connected with the outlet of the filter, and the other end of the output pipeline is connected with the collecting tank. The utility model reduces the filtering time of the liquid to be processed and improves the filtering quality and the filtering efficiency of the liquid to be processed in the filter; high-pressure flushing of the filter is achieved.

Description

Protein purification system

Technical Field

The utility model relates to the technical field of biological purification, in particular to a protein purification system.

Background

In the purification process of the biological industry, a protein purification system (namely an AKTA system) is often used, and in the process of protein purification, a plunger pump is used together with a chromatographic column, so that the chromatographic column has simple and economical technology and good separation effect and is applied to the extraction, separation and purification of natural products. However, when the device is used for filtering liquid, the chromatographic column is replaced by a filter, the plunger pump drives the liquid to enter the filter for filtering, the plunger pump cannot completely simulate the production operation, and the liquid cannot reach the preset flow rate due to the limitation of the pump speed of the plunger pump, so that the filtering time of the liquid is longer, and the quality of a product is reduced.

In view of this, a protein purification system is needed to solve the above-mentioned problems.

Disclosure of Invention

Based on the above, an object of the present utility model is to provide a protein purification system, which reduces the filtration time of the liquid to be processed, and improves the filtration quality and filtration efficiency of the liquid to be processed in the filter; high-pressure flushing of the filter is achieved.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a protein purification system comprising:

the device comprises a first buffer tank, a second buffer tank and a first control unit, wherein the first buffer tank stores liquid to be processed;

the inlet of the peristaltic pump is connected with the outlet of the first buffer tank;

a filter for filtering the liquid to be processed;

the first branch comprises a second buffer tank and a plunger pump, and an inlet of the plunger pump is connected with an outlet of the second buffer tank;

a first multi-way valve connected to the outlet of the plunger pump, the outlet of the peristaltic pump and the inlet of the filter, respectively;

the output pipeline and the collection tank, output pipeline one end connect in the export of filter, the other end connect in the collection tank.

As a preferred technical scheme of the protein purification system, the system further comprises a second multi-way valve, wherein the second multi-way valve is respectively connected with the first multi-way valve, the inlet of the filter, the outlet of the filter and the output pipeline;

the second multi-way valve comprises a first working position and a second working position, when the second multi-way valve is positioned in the first working position, the first multi-way valve is communicated with the inlet of the filter through the second multi-way valve, and the outlet of the filter is communicated with the output pipeline through the second multi-way valve; and when the second multi-way valve is in the second working position, the first multi-way valve is communicated with the output pipeline through the second multi-way valve.

As a preferred technical scheme of the protein purification system, the system further comprises a plurality of third multi-way valves, wherein the third multi-way valves are respectively connected with the inlet of the peristaltic pump and the outlets of the first multi-way valves.

As a preferred technical scheme of the protein purification system, the first branch further comprises a fourth multi-way valve, the second buffer tanks are multiple, and the fourth multi-way valve is respectively connected with the inlet of the plunger pump and the outlets of the second buffer tanks.

As a preferred technical scheme of the protein purification system, the first branch is multiple, and the first multi-way valve is connected to the outlets of the plunger pumps in the multiple first branches.

As a preferred technical scheme of the protein purification system, the outlet of the peristaltic pump is connected with a first pressure detection piece, and the first pressure detection piece is used for detecting the pressure of the outlet of the peristaltic pump.

As a preferred technical scheme of the protein purification system, the outlet of the plunger pump is connected with a second pressure detection piece, and the second pressure detection piece is used for detecting the pressure of the outlet of the plunger pump.

As a preferred embodiment of the protein purification system, the filter is a chromatographic column.

As a preferable technical scheme of the protein purification system, the output pipeline is provided with a detection sensor which is used for detecting the processing quality of the liquid to be processed.

As a preferred embodiment of the protein purification system, the detection sensor comprises an ultraviolet detector, a PH detector and/or a conductivity detector.

The beneficial effects of the utility model are as follows:

the utility model provides a protein purification system, which is characterized in that a peristaltic pump is connected to an inlet of a filter through a first multi-way valve when in operation, liquid to be processed in a first buffer tank is driven by the peristaltic pump and enters the filter, the peristaltic pump can increase the flow rate of the liquid to be processed, a plunger pump in the prior art is changed into the peristaltic pump, the flow rate is not limited by the pump speed of the plunger pump any more, the actual production operation is simulated, the filtering time of the liquid to be processed is shortened, the filtering quality and the filtering efficiency of the liquid to be processed in the filter are improved, and the filtered liquid is discharged through an outlet of the filter and enters a collecting tank through an output pipeline.

Furthermore, because the filter uses the back for a long time, impurity increases on the filtration membrane in the filter, leads to the permeability of filtration membrane to reduce, need use the flushing fluid of high pressure to wash the filter, but, peristaltic pump can't satisfy the requirement of washing pressure, at this moment, the plunger pump is connected in the import of filter through first multiport valve, output line and collection tank circuit break, the plunger pump can increase the pressure of flushing fluid, through plunger pump drive flushing fluid, realized the high pressure flushing to the filter, filter exhaust flushing fluid is discharged through output line.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the following description will briefly explain the drawings needed in the description of the embodiments of the present utility model, and it is obvious that the drawings in the following description are only some embodiments of the present utility model, and other drawings may be obtained according to the contents of the embodiments of the present utility model and these drawings without inventive effort for those skilled in the art.

FIG. 1 is a schematic diagram of a protein purification system provided in an embodiment of the present utility model.

The figures are labeled as follows:

1. a first buffer tank; 2. a peristaltic pump; 3. a filter;

4. a first branch; 41. a second buffer tank; 42. a plunger pump; 43. a fourth multi-way valve; 44. a second pressure detecting member;

5. a first multi-way valve; 6. an output line; 7. a second multi-way valve; 8. a third multi-way valve; 9. a first pressure detecting member;

101. an ultraviolet detector; 102. a PH detector; 103. conductivity detector.

Detailed Description

The utility model is described in further detail below with reference to the drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the utility model and are not limiting thereof. It should be further noted that, for convenience of description, only some, but not all of the structures related to the present utility model are shown in the drawings.

In the description of the present utility model, unless explicitly stated and limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

In the present utility model, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, as well as the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.

In the description of the present embodiment, the terms "upper", "lower", "left", "right", and the like are orientation or positional relationships based on those shown in the drawings, merely for convenience of description and simplicity of operation, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the utility model. Furthermore, the terms "first," "second," and the like, are used merely for distinguishing between descriptions and not for distinguishing between them.

As shown in fig. 1, the present embodiment provides a protein purification system comprising a first buffer tank 1, a peristaltic pump 2, a plunger pump 42, a filter 3, a first branch 4, a first multi-way valve 5, an output line 6, and a collection tank.

Specifically, the first buffer tank 1 stores a liquid to be processed; the inlet of the peristaltic pump 2 is connected with the outlet of the first buffer tank 1; the filter 3 is used for filtering the liquid to be processed; the first branch 4 comprises a second buffer tank 41 and a plunger pump 42, wherein an inlet of the plunger pump 42 is connected with an outlet of the second buffer tank 41; the first multi-way valve 5 is connected to the outlet of the plunger pump 42, the outlet of the peristaltic pump 2 and the inlet of the filter 3, respectively; one end of the output pipeline 6 is connected to the outlet of the filter 3, the other end of the output pipeline 6 is connected to the collecting tank, and the on-off state can be adjusted between the output pipeline 6 and the collecting tank. In this embodiment, the first buffer tank 1, the peristaltic pump 2, the filter 3, the first branch 4, the output pipeline 6 and the collection tank are all communicated through pipelines. In this embodiment, the first multi-way valve 5 is a three-way valve, and has three working ports, the filter 3 filters the liquid to be processed, and the first multi-way valve 5 is in the prior art, and the specific structure and working principle thereof are not described again. The disconnection and passage between the output pipe 6 and the collection tank can be regulated by the valve body.

During operation, peristaltic pump 2 is connected in the import of filter 3 through first multiport valve 5, the liquid that waits to process in the first buffer tank 1 passes through peristaltic pump 2 drive and gets into in the filter 3, peristaltic pump 2 can increase the velocity of flow of waiting to process the liquid, reform transform the plunger pump of prior art into peristaltic pump 2, the velocity of flow no longer receives the restriction of plunger pump speed, simulate actual production operation, the filtration time of waiting to process the liquid has been reduced, the filtration quality and the filtration efficiency of waiting to process the liquid in filter 3 have been improved, the liquid after the filtration is discharged through the export of filter 3, and get into the collecting vessel through output pipeline 6. Furthermore, because the filter 3 is used for a long time, the impurities on the filtering membrane in the filter 3 are increased, the permeability of the filtering membrane is reduced, the filter 3 is required to be flushed by high-pressure flushing fluid, however, the peristaltic pump 2 cannot meet the requirement of flushing pressure, at this time, the flushing fluid is stored in the second buffer tank 41, the plunger pump 42 is connected to the inlet of the filter 3 through the first multi-way valve 5, the output pipeline 6 is disconnected from the collecting tank, the plunger pump 42 can increase the pressure of the flushing fluid, the flushing fluid is driven by the plunger pump 42, the flushing of the filter 3 is realized, and the flushing fluid discharged by the filter 3 is discharged through the output pipeline 6.

In this embodiment, the filter 3 is a chromatographic column, and the filter 3 is replaced with a chromatographic column. When the chromatographic column works, a large liquid pressure is needed, so that in the working process, the liquid to be processed is stored in the second buffer tank 41, the plunger pump 42 is connected to the inlet of the filter 3 through the first multi-way valve 5, and the plunger pump 42 can increase the conveying pressure of the liquid to be processed, so that the liquid to be processed can be separated and purified in the chromatographic column. In the embodiment, the protein purification system not only can filter the liquid to be processed, but also can carry out chromatographic processing on the liquid to be processed, so that the versatility of the protein purification system is improved. Wherein, the plunger pump 42 is matched with the chromatographic column, and the principle is the same as that of the traditional chromatographic equipment, so that the original chromatographic function is maintained; furthermore, when the chromatographic column is replaced by the filter 3, and the peristaltic pump 2 is integrated into the traditional chromatographic equipment, the membrane filtration function of the peristaltic pump 2 is realized.

In this embodiment, the first branch 4 is plural, and the first multi-way valve 5 is connected to the outlets of the plunger pumps 42 in the plural first branches 4.

The lines of the protein purification system need to be flushed prior to filtration, but no separate cleaning of the lines is possible with existing protein purification systems. In this embodiment, the protein purification system further comprises a second multi-way valve 7, wherein the second multi-way valve 7 is connected to the first multi-way valve 5, the inlet of the filter 3, the outlet of the filter 3 and the output pipeline 6, respectively; the second multi-way valve 7 comprises a first working position and a second working position, when the second multi-way valve 7 is in the first working position, the first multi-way valve 5 is communicated with the inlet of the filter 3 through the second multi-way valve 7, and the outlet of the filter 3 is communicated with the output pipeline 6 through the second multi-way valve 7; when the second multi-way valve 7 is in the second operating position, the first multi-way valve 5 is connected to the output line 6 via the second multi-way valve 7. When the second multi-way valve 7 is in the first working position, the liquid to be processed can be filtered; the liquid to be processed can be conveyed into the filter 3 through the second multi-way valve 7, so that the liquid to be processed is filtered, and the filtered liquid is conveyed into the collecting tank through the second multi-way valve 7 and the conveying pipeline. When the second multi-way valve 7 is in the second work, the pipeline can be flushed, and flushing liquid directly flows into the conveying pipeline through the second multi-way valve 7, so that the flushing efficiency is improved.

In this embodiment, the second multi-way valve 7 includes a first working port, a second working port, a third working port and a fourth working port, which are respectively connected to the first multi-way valve 5, the inlet of the filter 3, the outlet of the filter 3 and the output pipeline 6; when the second multi-way valve 7 is in the first working position, the first working port is communicated with the second working port, and the third working port is communicated with the fourth working port. When the second multi-way valve 7 is in the first operating position, only the first operating port communicates with the fourth operating port. The second multi-way valve 7 is of prior art.

Preferably, the protein purification system further comprises a third multi-way valve 8, the first buffer tank 1 being a plurality, the third multi-way valve 8 being connected to the inlet of the peristaltic pump 2 and the outlet of the plurality of first buffer tanks 1, respectively. The third multi-way valve 8 can automatically switch the first buffer tank 1 communicated with the peristaltic pump 2, so that the bearing capacity of liquid to be processed is improved, and the working efficiency is improved.

Further preferably, the first branch 4 further comprises a fourth multi-way valve 43, the second buffer tanks 41 being plural, the fourth multi-way valve 43 being connected to an inlet of the plunger pump 42 and an outlet of the plural second buffer tanks 41, respectively. The structure and the working principle of the fourth multi-way valve 43 are the same as those of the third multi-way valve 8, and the third multi-way valve 8 and the fourth multi-way valve 43 are both in the prior art, and the specific structure and the working principle thereof are not described again.

In the prior art, peristaltic pumps are offline data and need to be manually recorded. In this embodiment, the outlet of the peristaltic pump 2 is connected to a first pressure detecting member 9, and the first pressure detecting member 9 is used for detecting the pressure at the outlet of the peristaltic pump 2. The first pressure detecting piece 9 realizes online recording of pressure data in the pipeline, saves labor cost, and can check a corresponding pressure map in software of the protein purification system after the experiment is finished through software setting.

Further preferably, a second pressure detecting member 44 is connected to the outlet of the plunger pump 42, and the second pressure detecting member 44 is configured to detect the pressure at the outlet of the plunger pump 42. Similarly, the second pressure sensing element 44 enables online recording of pressure data in the line of the first branch 4. In this embodiment, the first pressure detecting member 9 and the second pressure detecting member 44 are pressure transmitters or pressure sensors.

Preferably, the output pipeline 6 is provided with a detection sensor, the detection sensor is used for detecting the processing quality of the liquid to be processed, when the detection sensor detects that the processing quality of the liquid to be processed meets the requirement, a passage between the collection tank and the output pipeline 6 is controlled, and the collection tank is used for collection, so that the product quality is improved. In this embodiment, the detection sensor includes an ultraviolet detector 101, a PH detector 102, and/or a conductivity detector 103. Before the protein purification system processes the liquid to be processed, the flushing liquid is arranged in the protein purification system, when the liquid to be processed is processed, the flushing liquid in the output pipeline 6 is replaced by the processed liquid, the PH detector 102 detects the PH change of the liquid, the conductivity detector 103 detects the change of the conductivity in the liquid, and at the moment, the processed liquid in the output pipeline 6 is judged through the PH detector 102 and the conductivity detector 103. When the ultraviolet detector 101 detects that the processed liquid contains impurity particles, it can be determined that the filter 3 is defective in filtration and the processing is necessary.

Note that the above is only a preferred embodiment of the present utility model and the technical principle applied. It will be understood by those skilled in the art that the present utility model is not limited to the particular embodiments described herein, but is capable of various obvious changes, rearrangements and substitutions as will now become apparent to those skilled in the art without departing from the scope of the utility model. Therefore, while the utility model has been described in connection with the above embodiments, the utility model is not limited to the embodiments, but may be embodied in many other equivalent forms without departing from the spirit or scope of the utility model, which is set forth in the following claims.

Claims

1. A protein purification system, comprising:

a first buffer tank (1), wherein the first buffer tank (1) stores liquid to be processed;

the peristaltic pump (2), the inlet of the peristaltic pump (2) is connected with the outlet of the first buffer tank (1);

-a filter (3) for filtering the liquid to be processed;

a first branch (4) comprising a second buffer tank (41) and a plunger pump (42), the inlet of the plunger pump (42) being connected to the outlet of the second buffer tank (41);

a first multi-way valve (5), the first multi-way valve (5) being connected to the outlet of the plunger pump (42), the outlet of the peristaltic pump (2) and the inlet of the filter (3), respectively;

the filter comprises an output pipeline (6) and a collecting tank, wherein one end of the output pipeline (6) is connected with the outlet of the filter (3), and the other end of the output pipeline is connected with the collecting tank.

2. The protein purification system according to claim 1, further comprising a second multi-way valve (7), the second multi-way valve (7) being connected to the first multi-way valve (5), the inlet of the filter (3), the outlet of the filter (3) and the output line (6), respectively;

the second multi-way valve (7) comprises a first working position and a second working position, when the second multi-way valve (7) is positioned in the first working position, the first multi-way valve (5) is communicated with the inlet of the filter (3) through the second multi-way valve (7), and the outlet of the filter (3) is communicated with the output pipeline (6) through the second multi-way valve (7); when the second multi-way valve (7) is in the second working position, the first multi-way valve (5) is communicated with the output pipeline (6) through the second multi-way valve (7).

3. The protein purification system according to claim 1, further comprising a third multi-way valve (8), wherein the first buffer tank (1) is a plurality, and wherein the third multi-way valve (8) is connected to the inlet of the peristaltic pump (2) and the outlets of the plurality of first buffer tanks (1), respectively.

4. The protein purification system according to claim 1, wherein the first branch (4) further comprises a fourth multi-way valve (43), the second buffer tank (41) being a plurality, the fourth multi-way valve (43) being connected to an inlet of the plunger pump (42) and to an outlet of the plurality of second buffer tanks (41), respectively.

5. Protein purification system according to claim 1, characterized in that the first branch (4) is a plurality, and the first multi-way valve (5) is connected to the outlets of the plunger pumps (42) in a plurality of the first branches (4).

6. Protein purification system according to claim 1, characterized in that the peristaltic pump (2) outlet is connected with a first pressure detection member (9), the first pressure detection member (9) being adapted to detect the pressure at the peristaltic pump (2) outlet.

7. Protein purification system according to claim 1, characterized in that the outlet of the plunger pump (42) is connected with a second pressure detection member (44), the second pressure detection member (44) being adapted to detect the pressure at the outlet of the plunger pump (42).

8. Protein purification system according to any one of claims 1-7, characterized in that the filter (3) is a chromatography column.

9. Protein purification system according to any one of claims 1-7, characterized in that a detection sensor is provided on the output line (6) for detecting the processing quality of the liquid to be processed.

10. The protein purification system of claim 9, wherein the detection sensor comprises an ultraviolet detector (101), a PH detector (102), and/or a conductivity detector (103).