CN219824176U - Collect centrifugation and collection device of exosome - Google Patents
Collect centrifugation and collection device of exosome Download PDFInfo
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- CN219824176U CN219824176U CN202321259577.5U CN202321259577U CN219824176U CN 219824176 U CN219824176 U CN 219824176U CN 202321259577 U CN202321259577 U CN 202321259577U CN 219824176 U CN219824176 U CN 219824176U
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- 210000001808 exosome Anatomy 0.000 title claims abstract description 38
- 238000005119 centrifugation Methods 0.000 title claims abstract description 16
- 238000007789 sealing Methods 0.000 claims abstract description 31
- 238000001914 filtration Methods 0.000 claims description 11
- 239000012528 membrane Substances 0.000 claims description 7
- 230000000149 penetrating effect Effects 0.000 claims description 6
- 241000233866 Fungi Species 0.000 abstract description 2
- 238000007664 blowing Methods 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 6
- 230000033001 locomotion Effects 0.000 description 5
- 230000008569 process Effects 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 210000002487 multivesicular body Anatomy 0.000 description 2
- 238000005199 ultracentrifugation Methods 0.000 description 2
- 206010064390 Tumour invasion Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000030741 antigen processing and presentation Effects 0.000 description 1
- 210000000270 basal cell Anatomy 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000009400 cancer invasion Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000012292 cell migration Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 210000003712 lysosome Anatomy 0.000 description 1
- 230000001868 lysosomic effect Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
Abstract
The utility model relates to the technical field of exosomes, and discloses a centrifugal and collecting device for collecting exosomes, which comprises a centrifuge tube, wherein a sealing component is arranged above the centrifuge tube, the collecting component is arranged inside the centrifuge tube, the sealing component comprises a sealing cover which is connected with the top of the centrifuge tube in a threaded manner, two vertical plates are fixedly arranged at the bottom of the sealing cover, threaded rods are connected with one side of each vertical plate opposite to each other in a threaded manner, and knobs are fixedly arranged on one side of each threaded rod opposite to each other. This collect centrifugation and collection device of exosome can make sealed lid behind threaded connection through setting up seal assembly, avoids the centrifuging tube when centrifuging, and sealed lid takes place to rotate even the condition that breaks away from, prevents that the centrifuging tube when centrifuging, and the solution spills the condition that leaks, directly lets the liquid of collecting flow into the collecting pipe automatically through setting up the collection subassembly in, reduces to operate artificially, avoids dying the fungus to and the exosome damage that the blowing leads to.
Description
Technical Field
The utility model relates to the technical field of exosomes, in particular to a centrifugation and collection device for collecting exosomes.
Background
The exosomes mainly originate from the multivesicular bodies formed by the invagination of the lysosome microparticles in cells, are released into extracellular matrixes after being fused with cell membranes through the outer membranes of the multivesicular bodies, and the functions of the exosomes depend on the cell types from which the exosomes are derived, can participate in aspects such as immune response of organisms, antigen presentation, cell migration, cell differentiation, tumor invasion and the like, and research shows that the exosomes from the tumor sources participate in the exchange of genetic information of tumor cells and basal cells, so that a great amount of new blood vessels are generated, and the growth and invasion of tumors are promoted.
The most common amplification of exosome extraction is ultracentrifugation, the solution of macromolecules is discarded by ultracentrifugation, the exosomes of small molecules are left in a filter membrane, the extracted exosomes are sucked out by a pipette gun conventionally, but the extraction method can be polluted when the exosomes are collected, the filter membrane is possibly punctured, the problem of low collection efficiency is caused, meanwhile, when the centrifuge tube is centrifuged, a sealing cover on the top wall of the centrifuge tube is only connected through threads, when the centrifuge tube is centrifugally rotated, the sealing cover is possibly loosened or even falls off, and the solution is leaked, so that a centrifugal and collecting device for collecting the exosomes is provided to solve the problem.
Disclosure of Invention
(one) solving the technical problems
Aiming at the defects of the prior art, the utility model provides the centrifugal and collecting device for collecting the exosomes, which has the advantages of ensuring the sealing of the centrifuge tube and the like, and solves the problem that the centrifuge tube of the conventional centrifugal and collecting device for collecting the exosomes possibly causes loosening or even falling of the sealing cover when the centrifuge tube rotates in a centrifugal way.
(II) technical scheme
In order to achieve the purposes of ensuring the sealing of the centrifuge tube and the like, the utility model provides the following technical scheme: the utility model provides a collect centrifugation and collection device of exosome, includes the centrifuging tube, the top of centrifuging tube is provided with seal assembly, the inside of centrifuging tube is provided with the collection subassembly.
The sealing assembly comprises a sealing cover which is connected to the top of the centrifugal tube in a threaded mode, two vertical plates are fixedly arranged at the bottom of the sealing cover, threaded rods are connected to one side, opposite to the other side, of the vertical plates in a threaded mode, knobs are fixedly arranged on the other side, opposite to the other side, of the threaded rods in a threaded mode, movable plates are fixedly connected to the outer sides of the threaded rods in a threaded mode, two sliding rods with two ends penetrating through and extending to the opposite sides of the two vertical plates are fixedly arranged on the opposite sides of the sliding rods, arc-shaped clamping blocks are fixedly arranged on the opposite sides of the sliding rods, filtering devices are arranged in the centrifugal tube, and limiting rings are fixedly arranged on the top, opposite sides of the filtering devices.
Further, collection subassembly is including setting up in filter equipment bottom and running through to its inside sliding hole, the inboard fixed mounting of sliding hole has sealing rubber piece, the bottom fixed mounting of centrifuging tube has one end to run through and extend to the inside collecting pipe of filter equipment.
Further, two through holes penetrating to the opposite sides of the vertical plates are formed in the opposite sides of the vertical plates, and the four sliding rods are respectively connected with the four through holes in a sliding mode.
Further, opposite sides of the two arc-shaped clamping blocks are respectively abutted against the left side and the right side of the centrifugal tube.
Further, the annular groove is formed in the top of the centrifugal tube, the limiting ring is located in the annular groove, and the top of the annular groove is communicated with the outside.
Further, the filtering device comprises a fixed pipe which is connected inside the centrifugal pipe in a sliding mode, and a filter membrane is fixedly arranged at the bottom of the fixed pipe.
Further, the outer surface of the collecting pipe is connected with the inner side of the sliding hole in a sliding mode.
(III) beneficial effects
Compared with the prior art, the utility model provides a centrifugation and collection device for collecting exosomes, which has the following beneficial effects:
this collect centrifugation and collection device of exosome can make sealed lid behind threaded connection through setting up seal assembly, avoids the centrifuging tube when centrifuging, and sealed lid takes place to rotate even the condition that breaks away from, prevents that the centrifuging tube when centrifuging, and the solution spills the condition that leaks, directly lets the liquid of collecting flow into the collecting pipe automatically through setting up the collection subassembly in, reduces to operate artificially, avoids dying the fungus to and the exosome damage that the blowing leads to.
Drawings
FIG. 1 is a schematic diagram of the structure of the present utility model;
FIG. 2 is an enlarged view of A in FIG. 1, which is a structural view of the present utility model;
FIG. 3 is a top view of a centrifuge tube, arcuate clamp blocks, filter device and stop collar in the present utility model configuration;
FIG. 4 is a top view of the filter device, sliding aperture and sealing rubber sheet of the present utility model.
In the figure: 1. centrifuging tube; 2. a seal assembly; 201. sealing cover; 202. a riser; 203. a threaded rod; 204. a knob; 205. a movable plate; 206. a slide bar; 207. arc clamping blocks; 208. a filtering device; 209. a limiting ring; 3. a collection assembly; 301. a sliding hole; 302. sealing rubber sheets; 303. and (5) collecting pipes.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to fig. 1-3, the present utility model provides a technical solution: the utility model provides a collect centrifugation and collection device of exosome, includes centrifuging tube 1, and the top of centrifuging tube 1 is provided with seal assembly 2, and the inside of centrifuging tube 1 is provided with collection assembly 3.
In this embodiment, the sealing assembly 2 is an assembly for sealing the centrifuge tube 1.
As shown in fig. 1, fig. 2, fig. 3 and fig. 4, the sealing component 2 comprises a sealing cover 201 which is connected with the top of the centrifuge tube 1 in a threaded manner, two vertical plates 202 are fixedly installed at the bottom of the sealing cover 201, threaded rods 203 are connected with one side of the two vertical plates 202, knobs 204 are fixedly installed on the opposite sides of the two threaded rods 203, movable plates 205 are connected with one side of the two threaded rods 203 in a threaded manner, sliding rods 206 with two ends penetrating through and extending to the opposite sides of the two vertical plates 202 are fixedly installed on the opposite sides of the two sliding rods 206 on the left side and the right side, arc-shaped clamping blocks 207 are fixedly installed on the opposite sides of the two sliding rods 206, a filtering device 208 is arranged in the centrifuge tube 1, and a limiting ring 209 is fixedly installed on the outer top of the filtering device 208.
Wherein, two through holes penetrating to opposite sides of the two vertical plates 202 are respectively formed on opposite sides of the two vertical plates 202, the four sliding rods 206 are respectively connected with the four through holes in a sliding manner, the stability of the movement of the sliding rods 206 can be ensured through the through holes, and meanwhile, the movable plates 205 can be prevented from rotating through the through holes and the sliding rods 206.
It should be noted that, when the two knobs 204 are turned, the two knobs 204 respectively drive the two threaded rods 203 to rotate, under the action of the thread thrust, the two threaded rods 203 respectively drive the two movable plates 205 to move to opposite sides, the two movable plates 205 respectively drive the two sliding rods 206 on the left side and the right side to move to opposite sides, and the two sliding rods 206 on the left side and the right side respectively drive the two arc-shaped clamping blocks 207 to move to opposite sides.
In addition, two opposite sides of arc clamp splice 207 respectively with the left and right sides butt of centrifuging tube 1, through the butt of two arc clamp splice 207 with centrifuging tube 1, can carry out spacingly to sealed lid 201, avoid when centrifuging, sealed lid 201 takes place to rotate or break away from.
It should be noted that, the annular groove is provided at the top of centrifuging tube 1, and stop collar 209 is located the inside of annular groove, and the top and the external world of annular groove are linked together, can support filter 208 through stop collar 209 and annular groove for filter 208 has placed more stably in centrifuging tube 1's inside.
Wherein, filter 208 includes sliding connection in the inside fixed pipe of centrifuging tube 1, and fixed pipe's bottom fixed mounting has the filter membrane, guarantees filter 208 motion's stability through fixed pipe, can separate filter 208 inside exosome through the filter membrane.
In this embodiment, the collection unit 3 is a unit for collecting the separated exosomes.
As shown in fig. 1 and 4, the collection assembly 3 includes a sliding hole 301 that is formed at the bottom of the filter 208 and extends into the filter, a sealing rubber sheet 302 is fixedly mounted on the inner side of the sliding hole 301, and a collection pipe 303 having one end extending into the filter 208 is fixedly mounted on the bottom of the centrifuge tube 1.
Wherein, the outer surface of the collecting pipe 303 is connected with the inner side of the sliding hole 301 in a sliding way, and the collecting pipe 303 can enter the inside of the filtering device 208 through the sliding hole 301, so as to collect the separated exosomes.
It should be noted that, when the collecting pipe 303 is not located inside the sliding hole 301, the four sealing rubber sheets 302 are horizontally distributed, and the four sealing rubber sheets 302 form a complete circle to seal the sliding hole 301, so that the exosome is prevented from flowing out through the sliding hole 301, when the collecting pipe 303 enters the sliding hole 301, the top of the collecting pipe 303 is extruded to separate the four sealing rubber sheets 302, and the sealing rubber sheets 302 are made of rubber materials and have a certain elasticity, and when the collecting pipe 303 is separated from the sliding hole 301, the sealing rubber sheets 302 return to the horizontal position to seal the sliding hole 301.
The working principle of the embodiment is as follows:
this collect centrifugation and collection device of exosome, when using, place filter equipment 208 in the inside of centrifuging tube 1, and make spacing ring 209 sliding connection in the ring channel inside, support filter equipment 208 through spacing ring 209 and ring channel, when putting into filter equipment 208, the top of collecting pipe 303 can stretch into filter equipment 208's inside through sliding hole 301 and sealing rubber piece 302, at this moment, with sealed lid 201 threaded connection in centrifuging tube 1's top, then rotate two knobs 204, two knobs 204 can drive two threaded rods 203 respectively and rotate, under the screw thrust effect, two threaded rods 203 can drive two fly leaf 205 respectively to opposite side motion, two fly leaf 205 can drive left side and right side two slide bars 206 respectively to opposite side motion, left side and right side two slide bars 206 can drive two arc clamp pieces 207 respectively to opposite side motion, make the opposite side of two arc clamp pieces 207 respectively with the left and right sides butt of 1, can carry out spacing to sealed lid 201, avoid when centrifuging, sealed lid takes place to rotate or break away from centrifuging tube 1, place high-speed centrifuge, place inside high-speed centrifuge, can reject the molecule through the exosome, can collect molecules, and the molecule is collected in the exosome, can be carried out the molecule.
The electrical components appearing herein are all electrically connected with the master controller and the power supply, the master controller can be a conventional known device for controlling a computer and the like, and the prior art of power connection is not described in detail herein.
It should be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The utility model provides a collect centrifugation and collection device of exosome, includes centrifuging tube (1), its characterized in that: a sealing assembly (2) is arranged above the centrifugal tube (1), and a collecting assembly (3) is arranged inside the centrifugal tube (1);
sealing component (2) are including threaded connection in sealed lid (201) at centrifuging tube (1) top, the bottom fixed mounting of sealed lid (201) has two risers (202), two equal threaded connection in opposite sides of riser (202) has threaded rod (203), two equal threaded connection in the outside of threaded rod (203) has fly leaf (205), two equal fixed connection in the outside of threaded rod (203) has slide bar (206) that two one ends run through and extend to two opposite sides of riser (202), left side and right side two equal fixed connection in opposite sides of slide bar (206) has arc clamp splice (207), the inside of centrifuging tube (1) is provided with filter equipment (208), the outside top fixed mounting of filter equipment (208) has spacing ring (209).
2. A centrifugation and collection apparatus for collecting exosomes according to claim 1, wherein: the collection assembly (3) comprises a sliding hole (301) which is formed in the bottom of the filtering device (208) and penetrates into the filtering device, a sealing rubber sheet (302) is fixedly arranged on the inner side of the sliding hole (301), and a collection pipe (303) with one end penetrating through and extending to the inside of the filtering device (208) is fixedly arranged at the bottom of the centrifugal tube (1).
3. A centrifugation and collection apparatus for collecting exosomes according to claim 1, wherein: two through holes penetrating to opposite sides of the vertical plates (202) are formed in opposite sides of the vertical plates, and four sliding rods (206) are respectively connected with the four through holes in a sliding mode.
4. A centrifugation and collection apparatus for collecting exosomes according to claim 1, wherein: opposite sides of the two arc-shaped clamping blocks (207) are respectively abutted against the left side and the right side of the centrifugal tube (1).
5. A centrifugation and collection apparatus for collecting exosomes according to claim 1, wherein: an annular groove is formed in the top of the centrifugal tube (1), the limiting ring (209) is located in the annular groove, and the top of the annular groove is communicated with the outside.
6. A centrifugation and collection apparatus for collecting exosomes according to claim 1, wherein: the filtering device (208) comprises a fixed pipe which is connected inside the centrifugal pipe (1) in a sliding mode, and a filter membrane is fixedly arranged at the bottom of the fixed pipe.
7. A centrifugation and collection apparatus for collecting exosomes according to claim 2, wherein: the outer surface of the collecting pipe (303) is connected with the inner side of the sliding hole (301) in a sliding way.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321259577.5U CN219824176U (en) | 2023-05-23 | 2023-05-23 | Collect centrifugation and collection device of exosome |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321259577.5U CN219824176U (en) | 2023-05-23 | 2023-05-23 | Collect centrifugation and collection device of exosome |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219824176U true CN219824176U (en) | 2023-10-13 |
Family
ID=88251993
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321259577.5U Active CN219824176U (en) | 2023-05-23 | 2023-05-23 | Collect centrifugation and collection device of exosome |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219824176U (en) |
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2023
- 2023-05-23 CN CN202321259577.5U patent/CN219824176U/en active Active
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