CN219792976U - In situ hybridization device with temperature sensor - Google Patents
In situ hybridization device with temperature sensor Download PDFInfo
- Publication number
- CN219792976U CN219792976U CN202320592231.0U CN202320592231U CN219792976U CN 219792976 U CN219792976 U CN 219792976U CN 202320592231 U CN202320592231 U CN 202320592231U CN 219792976 U CN219792976 U CN 219792976U
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- temperature sensor
- reaction tank
- sensor
- situ hybridization
- fixing seat
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- 238000007901 in situ hybridization Methods 0.000 title claims abstract description 27
- 238000006243 chemical reaction Methods 0.000 claims abstract description 73
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 31
- 238000007789 sealing Methods 0.000 claims description 12
- 239000004696 Poly ether ether ketone Substances 0.000 claims description 4
- 229920002530 polyetherether ketone Polymers 0.000 claims description 4
- JUPQTSLXMOCDHR-UHFFFAOYSA-N benzene-1,4-diol;bis(4-fluorophenyl)methanone Chemical compound OC1=CC=C(O)C=C1.C1=CC(F)=CC=C1C(=O)C1=CC=C(F)C=C1 JUPQTSLXMOCDHR-UHFFFAOYSA-N 0.000 claims description 3
- 238000005265 energy consumption Methods 0.000 abstract 1
- 239000012774 insulation material Substances 0.000 abstract 1
- 238000009529 body temperature measurement Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 238000009396 hybridization Methods 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 230000007797 corrosion Effects 0.000 description 2
- 238000005260 corrosion Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002853 nucleic acid probe Substances 0.000 description 2
- 230000005856 abnormality Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 210000001726 chromosome structure Anatomy 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000002509 fluorescent in situ hybridization Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000000565 sealant Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides an in-situ hybridization device with a temperature sensor, wherein the temperature sensor for detecting the temperature of a reagent in a reaction tank is assembled in a fixed seat at the bottom of the reaction tank, and the top end of the temperature sensor is arranged in the reaction tank and is 1-1.5 mm away from the bottom of the reaction tank, so that the reagent consumption can be saved, and the temperature control energy consumption can be reduced. The fixing seat adopts heat insulation materials to reduce the interference of the temperature sensor on the tank body of the reaction tank, so that the temperature sensor is more accurate. The temperature sensor is assembled on the fixing seat by adopting the joint combination, so that the temperature sensor is convenient to maintain and replace rapidly when the temperature sensor fails.
Description
Technical Field
The utility model belongs to the field of biological detection devices, and particularly relates to in-situ hybridization equipment with a temperature sensor.
Background
Fluorescent in situ hybridization is a technology commonly used for detecting the position of a specific nucleic acid in molecular biology experiments, and can be applied to practical application aspects such as gene map drawing, chromosome structure abnormality observation, oncology gene analysis and the like. The principle of fluorescence in situ hybridization is that a nucleic acid probe with a fluorescent label is combined with a target gene to form a hybridization combination of target DNA and the nucleic acid probe, so that the target gene is observed under a fluorescence microscope.
In situ hybridization involves DNA denaturation and hybridization, which requires temperature control to denature DNA molecules between 72℃and 95℃and hybridization at 37℃or 42 ℃. At present, a special instrument device for fluorescence in-situ hybridization is arranged on the market, a reagent is loaded in a reaction tank, a temperature sensor is arranged in the reaction tank to transmit the temperature of the reagent, and a temperature control element such as a TEC (thermoelectric cooler), a radiator and the like below the reaction tank are used for controlling the temperature, so that the steps of denaturation and hybridization can be realized.
The existing in situ hybridization device refers to FAS-1000, temperature loss and reagent evaporation can be reduced by closing the reaction tank, but as the temperature sensor is directly arranged on the side surface of the reaction tank and passes through the wall of the reaction tank to contact with the reagent in the reaction tank, no heat insulation measures are provided between the temperature sensor and the reaction tank, and the temperature sensor is sealed by sealant, so that the following defects are brought: because the temperature response of the reaction tank is faster than that of the reagent, the temperature sensor detects the temperature of the reagent, and the temperature sensor is affected by the wall of the reaction tank to cause inaccurate transmission and thus inaccurate temperature control; because the temperature measurement of at least 3.5mm from the top of the temperature sensor to the bottom of the reaction tank requires the reagent to completely submerge the sensor, the required reagent amount is large, the reagent waste is caused, and the temperature control power consumption is large; the sealing glue is adhered to the wall of the reaction tank, so that the temperature sensor is not easy to maintain and replace when the temperature sensor fails. Patent CN208266182 has the problem that the temperature sensor is mounted on the side.
Disclosure of Invention
The utility model provides an in-situ hybridization device, which improves a temperature element for detecting the temperature of a reagent in a reaction tank of the existing in-situ hybridization device, and solves the problems of inaccurate temperature measurement, large consumption of required reaction reagent and high temperature control power consumption of the reaction tank of the existing in-situ hybridization device.
In order to achieve the above purpose, the present utility model adopts the following technical scheme:
an in situ hybridization device comprises a reaction tank and a temperature sensor for detecting the temperature of a reagent in the reaction tank, wherein the temperature sensor is arranged on the bottom surface of the reaction tank; the top end of the temperature sensor is positioned in the reaction tank.
Further, the top end of the temperature sensor is 1-1.5 mm away from the inner bottom surface of the reaction tank. The design can effectively reduce the required reagent amount, save the reagent and reduce the temperature control power consumption.
Further, the in situ hybridization device also comprises a temperature sensor fixing seat fixed on the bottom surface of the reaction tank, a joint combination for fixing the temperature sensor and an O-shaped sealing ring.
Further, the temperature sensor fixing seat is made of PEEK. The material is totally called polyether-ether-ketone and has the advantages of high temperature resistance, corrosion resistance, ageing resistance, easiness in processing and the like. The temperature sensor fixing seat can be applied to the temperature sensor fixing seat, can insulate heat around the temperature sensor to prevent the temperature measurement from being influenced, and has good reagent biocompatibility. Thereby improving the temperature measurement accuracy of the temperature sensor.
Further, the temperature sensor fixing seat comprises a positioning boss, a sensor positioning hole positioned in the positioning boss and a sensor hole shaft positioned under the positioning boss, wherein the diameter of the sensor hole shaft is larger than that of the sensor positioning hole, so that the diameter of the sensor positioning hole is equal to that of the sensor, and reagent residues are reduced. The positioning boss is matched with a positioning hole at the bottom of the reaction tank, and the temperature sensor fixing seat is fixed at the bottom of the reaction tank by two screws.
Further, the temperature sensor passes through the sensor positioning hole and the sensor hole shaft, and is fixed at the sensor hole shaft by the joint combination, and the top end of the temperature sensor passes through the positioning hole at the bottom of the reaction tank, so that the top end of the temperature sensor is positioned in the reaction tank. The diameter of the temperature sensor is the same as the inner diameter of the sensor positioning hole, so that reagent residues are reduced.
Further, the joint combination comprises a joint and a snap ring type nut, the temperature sensor is sleeved with the joint and then is assembled in the fixing seat, the snap ring type nut is assembled on the sensor hole shaft and is connected with the temperature sensor fixing seat through threads, the joint is extruded after the snap ring type nut is screwed, so that the joint is deformed, and sealing between the temperature sensor and the temperature sensor fixing seat and sealing between the joint inner hole and the outer wall of the temperature sensor are realized. The temperature sensor is fixed by adopting the joint combination, and when the temperature sensor fails, the damaged temperature sensor can be taken out by loosening the snap ring type nut, and the temperature sensor is replaced and then reassembled, so that the rapid maintenance is realized.
Further, the outer wall of the positioning boss on the temperature sensor fixing seat comprises an O-shaped sealing ring, and the temperature sensor fixing seat is sealed with the reaction groove by utilizing the O-shaped sealing ring.
Further, the positioning boss is flush with the inner bottom surface of the reaction tank in the assembled state. Can realize the evacuation of reagent when the reaction tank flowing back, avoid reagent to leave over.
Compared with the prior art, the utility model has the following beneficial effects:
the utility model provides an in situ hybridization device, wherein a temperature sensor is assembled at the bottom of a reaction tank through a sensor fixing seat, the top end of the sensor is arranged in the reaction tank and is 1-1.5 mm away from the bottom of the reaction tank, compared with the mode that the temperature sensor is fixed at the side surface of the reaction tank and is 3-4 mm away from the bottom in the prior art, the in situ hybridization device has the advantages that the required reagent amount in the reaction tank is reduced, the volume of the reaction tank is reduced, and the effect of reducing the temperature control power consumption is realized. The temperature sensor fixing seat is made of high-temperature-resistant and corrosion-resistant PEEK material, and is used for insulating the temperature sensor, so that compared with the prior art; the temperature sensor is directly assembled on the inner wall of the reaction tank, so that the temperature of the reaction tank body is prevented from influencing the transmission of the temperature sensor to the temperature of the reagent, and the defects of inaccurate temperature measurement and inaccurate temperature control are overcome.
The utility model also has the advantage of easy replacement, the joint combination for fixing the temperature sensor can be taken down when the temperature sensor fails, and the new temperature sensor is replaced to realize the rapid maintenance of the reaction tank.
Drawings
Fig. 1: schematic structural diagram of reaction tank
Fig. 2: schematic diagram of assembly mode of fixing seat
Fig. 3: structure schematic diagram of fixing seat
Fig. 4: schematic diagram of temperature sensor assembled on fixing seat
Fig. 5: schematic diagram of temperature sensor assembly mode
Reference numerals in the drawings: 1. a reaction tank; 2. a temperature sensor fixing seat; 201. a sensor positioning hole; 202. a sensor bore shaft; 203. positioning the boss; 3. a joint assembly; 301. a joint; 302. a clasp type nut; 4. a temperature sensor; 5. an O-shaped sealing ring; 6. a screw; 7. the reaction tank is provided with a positioning hole.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.
Referring to FIGS. 1 to 5, the present utility model provides an in situ hybridization apparatus comprising a reaction tank 1 and a temperature sensor 4 for detecting the temperature of a reagent in the reaction tank. The reagents in the reaction tank are generally reagents for nucleic acid hybridization.
As shown in fig. 1 and 4, the temperature sensor 4 is installed at the bottom surface of the reaction tank 1; the top end of the temperature sensor 4 is positioned in the reaction tank 1. The top end of the temperature sensor 4 is 1-1.5 mm away from the inner bottom surface of the reaction tank 1. The temperature sensor 4 is fixed by the temperature sensor fixing seat 2 and the joint combination 3.
As shown in fig. 2, the positioning boss 203 of the temperature sensor fixing base 2 is sealed with the reaction tank 1 by an O-ring 5. The positioning boss 203 is assembled with the reaction tank positioning hole 7, the outer diameter of the positioning boss 203 is the same as the inner diameter of the reaction tank positioning hole 7, and the positioning boss 203 and the reaction tank positioning hole 7 cooperate to reduce reagent residues. The temperature sensor fixing seat 2 is fixed at the bottom of the reaction tank 1 by a screw 6.
As shown in fig. 3, a positioning boss 203 on the temperature sensor fixing seat 2 is provided with a sensor positioning hole 201 and a sensor hole shaft 202 communicated with the sensor positioning hole 201, and the inner wall of the sensor hole shaft 202 is threaded and can be connected with a snap ring type nut 302.
As shown in fig. 4, the joint combination 3 is composed of a joint 301 and a clasp nut 302. The temperature sensor 4 passes through the sensor hole shaft 202 and the sensor positioning hole 201, the top end of the temperature sensor reaches the inside of the reaction tank, and is fixed in the temperature sensor fixing seat 2 by the joint 301 and the snap ring type nut 302. The diameter of the outer wall of the probe of the temperature sensor 4 is the same as the inner diameter of the sensor positioning hole 201, so that reagent residue can be reduced. The joint combination 3 is a standard component in a fluid hard pipe connection scheme, wherein the clamping ring type nut 302 is in threaded connection with the sensor hole shaft 202, and the joint 301 is extruded to deform after being screwed, so that the sealing between the temperature sensor 4 and the temperature sensor fixing seat 2 and the sealing between the inner hole of the joint 301 and the outer wall of the temperature sensor 4 are realized.
The assembly sequence of all the devices is shown in fig. 5, and the positioning boss 203 on the temperature sensor fixing seat 2 is assembled with the positioning hole 7 on the bottom surface of the reaction tank, and the positioning hole are sealed by the O-shaped sealing ring 5; the whole temperature sensor fixing seat 2 is fixed at the bottom of the reaction tank 1 by two screws 6; the joint 301 is sleeved on the temperature sensor 4, passes through the sensor hole shaft 202 and the sensor positioning hole 201, and is adjusted to be 1-1.5 mm away from the bottom surface of the reaction tank at the top end of the temperature sensor 4, and is screwed and fixed in the temperature sensor fixing seat 2 by the snap ring type nut 302.
The in situ hybridization device transfers the original temperature sensor for detecting the temperature of the reagent in the reaction tank to the position 3.5-4 mm away from the bottom surface from the side surface of the reaction tank, protrudes 1-1.5 mm from the bottom surface of the reaction tank, and a temperature sensor fixing seat is added to insulate heat between the temperature sensor and the tank body of the reaction tank, thereby realizing the beneficial effects of saving reagent, reducing temperature control power consumption and improving temperature measurement accuracy.
The foregoing is only a preferred embodiment of the present utility model, but the scope of the present utility model is not limited thereto, and any person skilled in the art, who is within the scope of the present utility model, should make equivalent substitutions or modifications according to the technical scheme of the present utility model and the inventive concept thereof, and should be covered by the scope of the present utility model.
Claims (9)
1. An in situ hybridization device with a temperature sensor is characterized by comprising a reaction tank and the temperature sensor for detecting the temperature of a reagent in the reaction tank, wherein the temperature sensor is arranged on the bottom surface of the reaction tank; the top end of the temperature sensor is positioned in the reaction tank.
2. The in situ hybridization apparatus according to claim 1, wherein the top end of the temperature sensor is 1 to 1.5mm from the inner bottom surface of the reaction vessel.
3. The in situ hybridization apparatus as claimed in claim 1, wherein the in situ hybridization apparatus further comprises a temperature sensor fixing seat fixed to a bottom surface of the reaction tank, a joint assembly for fixing the temperature sensor, and an O-ring.
4. The in situ hybridization apparatus as claimed in claim 3, wherein the temperature sensor holder is made of PEEK.
5. The in situ hybridization apparatus as claimed in claim 3, wherein the temperature sensor fixing base comprises a positioning boss, a sensor positioning hole in the positioning boss, and a sensor hole shaft under the positioning boss, wherein an inner diameter of the sensor hole shaft is larger than a diameter of the sensor positioning hole, so that an inner diameter of the sensor positioning hole is equal to a diameter of an outer wall of the temperature sensor.
6. The in situ hybridization apparatus of claim 5, wherein the temperature sensor passes through the sensor hole shaft and the sensor positioning hole, is fixed at the sensor hole shaft by a joint assembly, and the top end of the temperature sensor passes through the positioning hole at the bottom of the reaction tank.
7. The in situ hybridization apparatus as claimed in claim 5, wherein the joint assembly comprises a joint and a clasp nut;
the joint is matched with a temperature sensor and is assembled in a sensor positioning hole in the fixing seat;
the snap ring type nut is assembled on the sensor hole shaft, is connected with the temperature sensor fixing seat through threads, and the temperature sensor extrudes the joint to realize sealing between the joint and the temperature sensor fixing seat and sealing between the joint inner hole and the outer wall of the temperature sensor.
8. The in situ hybridization device as claimed in claim 5, wherein the outer wall of the positioning boss on the temperature sensor fixing seat comprises an O-ring, and the temperature sensor fixing seat is sealed with the reaction tank by using the O-ring.
9. The in situ hybridization device as claimed in claim 5, wherein the top end of the positioning boss is flush with the inner bottom surface of the reaction tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320592231.0U CN219792976U (en) | 2023-03-23 | 2023-03-23 | In situ hybridization device with temperature sensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320592231.0U CN219792976U (en) | 2023-03-23 | 2023-03-23 | In situ hybridization device with temperature sensor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219792976U true CN219792976U (en) | 2023-10-03 |
Family
ID=88151662
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320592231.0U Active CN219792976U (en) | 2023-03-23 | 2023-03-23 | In situ hybridization device with temperature sensor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219792976U (en) |
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2023
- 2023-03-23 CN CN202320592231.0U patent/CN219792976U/en active Active
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