CN219770231U - Be applied to automatic sealing membrane and dyestripping's of amplification instrument device - Google Patents
Be applied to automatic sealing membrane and dyestripping's of amplification instrument device Download PDFInfo
- Publication number
- CN219770231U CN219770231U CN202320462573.0U CN202320462573U CN219770231U CN 219770231 U CN219770231 U CN 219770231U CN 202320462573 U CN202320462573 U CN 202320462573U CN 219770231 U CN219770231 U CN 219770231U
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- Prior art keywords
- test tube
- automatic sealing
- tearing
- push
- amplification instrument
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- 238000007789 sealing Methods 0.000 title claims abstract description 36
- 230000003321 amplification Effects 0.000 title claims abstract description 24
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 24
- 239000012528 membrane Substances 0.000 title claims description 5
- 238000012360 testing method Methods 0.000 claims abstract description 30
- 238000001514 detection method Methods 0.000 description 12
- 150000007523 nucleic acids Chemical class 0.000 description 6
- 102000039446 nucleic acids Human genes 0.000 description 6
- 108020004707 nucleic acids Proteins 0.000 description 6
- 230000003287 optical effect Effects 0.000 description 5
- 239000007788 liquid Substances 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 2
- 230000000712 assembly Effects 0.000 description 2
- 238000000429 assembly Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000980 acid dye Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000013013 elastic material Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/62—Plastics recycling; Rubber recycling
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a device applied to automatic sealing and film tearing of an amplification instrument, which comprises a body, wherein a sample tank is arranged on an upper platform of the body, a test tube die table is arranged in the sample tank, a sealing cover is rotationally arranged in the sample tank and used for covering the sample tank so as to enable the test tube die table to be in a sealing space, a cover plate placing cabin is arranged on the sealing cover, a plug cover is arranged on the cover plate placing cabin, a push rod assembly which is arranged in a rectangular array is arranged on the plug cover, the push rod assembly comprises a push block, and a sucker is arranged on one side of the push block, which faces the test tube die table. The device that is applied to automatic envelope and the dyestripping of amplification instrument that this practicality provided, simple structure, convenient operation is swift, utilizes negative pressure and pressure boost can realize envelope and the dyestripping operation to the test tube.
Description
Technical Field
The utility model relates to the technical field of amplification instruments, in particular to a device applied to automatic sealing and tearing of an amplification instrument.
Background
The amplification instrument belongs to a common biological laboratory instrument, and consists of a control component, a thermal circulation component, a sample cell and a sample sealing cover. In practical use, liquid is put into a 96-well plate with the volume of 0.2ml, an eight-connecting tube with the volume of 0.2ml or a single tube with the volume of 0.2ml through a pipetting test tube, and then a sample sealing cover is used for sealing, so that a test tube cover plate placed in the sample sealing cover can be buckled on the test tube. At this time, after the control part sets the expansion temperature, the temperature changing rate, the gradient and the like, the thermal circulation part carries out heating treatment on the liquid in the test tube, and the prior art application of the detailed amplification instrument is referred to as the following patent:
publication (bulletin) number: CN114181809a, publication (date): 2022-03-15 discloses a nucleic acid amplification product quantitative detection instrument and a nucleic acid amplification product quantitative detection method, comprising an optical detection module and a detection control module, and characterized in that: the detection instrument comprises an optical detection module shell, wherein a sample hole for accommodating the PCR amplification tube is formed in the optical detection module shell, light-passing holes which are vertically intersected with the sample hole are formed in the two sides of the sample hole in the optical detection module shell, and a light source and a photoelectric sensor are respectively arranged in the light-passing holes in the two sides of the optical detection module shell; the utility model directly uses a nucleic acid amplification reaction container (PCR amplification tube) as a cuvette to quantitatively detect the nucleic acid amplification product, solves the defects that the quantitative detection result cannot be obtained by observing the color development result of the nucleic acid dye by naked eyes, and the spectrophotometer needs to transfer sample liquid to cause time and labor waste, pollution and false positive result are easy to cause in the detection process, and realizes the rapid quantitative detection of the nucleic acid amplification product.
In the prior art including above-mentioned prior art patent, equipment all needs to advance the closing cap to the test tube in the operation in-process, but need the manual work to detain after the closing cap and remove the closing cap, and experimental environment need go on under aseptic environment, and research and development personnel wear disposable gloves, go in proper order to operate detain and remove the closing cap and be rather difficult in fact.
Disclosure of Invention
The utility model aims to provide a device applied to automatic sealing and film tearing of an amplification instrument, which enables the device to have the capability of sealing and film tearing of a test tube port.
In order to achieve the above object, the present utility model provides the following technical solutions: the utility model provides a be applied to automatic sealing membrane of amplification instrument and device of dyestripping, includes the body, has seted up the sample cell on its upper platform, be provided with test tube die bench in the sample cell, the sample cell internal rotation is provided with the closing cap, the closing cap is used for covering the sample cell so that test tube die bench is in sealed space, be provided with the apron on the closing cap and place the cabin, be provided with the cock cap on the cabin is placed to the apron, be provided with the ejector pin subassembly that is rectangular array and arrange on the cock cap, the ejector pin subassembly is including supporting the ejector pin piece, it is towards to support the ejector pin piece test tube die bench one side is provided with the sucking disc.
Preferably, the number of the ejector rod assemblies is consistent with that of the test tube holes of the test tube die table.
Preferably, a limiting frame is arranged at one end of the cover plate placing cabin facing the sample tank, and the end part of the pushing block is flush with one side of the limiting frame facing the cover plate placing cabin.
Preferably, the ejector rod assembly comprises a mounting rod which is connected to the plug cover in a threaded manner and is communicated with an air cavity in the plug cover;
the ejector rod assembly further comprises a piston rod, wherein the piston rod is movably arranged in the mounting rod and is assembled with the pushing block in a threaded manner.
Preferably, the end part of the piston rod is provided with a one-way valve formed by stacking a plurality of arc-shaped side lobes, and the outer arc top of the one-way valve faces one side of the air cavity.
Preferably, an elastic member is provided between the mounting rod and the piston rod.
Preferably, the suction cup comprises a central part, an elastic connection part and an edge ring, wherein the elastic connection part is used for connecting the central part and the edge ring;
the pushing block is movably provided with a pushing ring moving along the vertical direction, the pushing ring is connected with the edge ring, and the sucker is switched between the following two modes:
in the first mode, the edge ring extends to the outer side of the end face of the pushing block, so that the sucker is horn-shaped;
in the second form, the edge ring and the central part are positioned at the same level and are both positioned in the pushing block.
Preferably, a plurality of guide blocks arranged in a circumferential array are slidably arranged in the pushing block, and a hook rod is rotatably arranged between the guide blocks and the pushing ring
Preferably, a bump is arranged in the pushing block, and the bump is rotationally connected with the hook rod and is arranged close to the push ring.
In the technical scheme, the device for automatically sealing and tearing the membrane for the amplification instrument has the following beneficial effects: the ejector rod assembly is communicated with an air cavity in the plug cover, and the cover plate placed in the cover plate placing cabin is covered by the ejector rod assembly and the test tube by utilizing pressurization, so that the sealing film is realized; or the cover plate is adsorbed to the sucker under negative pressure so as to realize film tearing.
Drawings
In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings required for the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments described in the present utility model, and other drawings may be obtained according to these drawings for a person having ordinary skill in the art.
FIG. 1 is a schematic diagram of an overall structure according to an embodiment of the present utility model;
FIG. 2 is a schematic cross-sectional view of a combination of a closure and a stopper according to an embodiment of the present utility model;
fig. 3 is an enlarged schematic view of a structure at a provided in an embodiment of the present utility model.
Reference numerals illustrate:
1. a test tube die table; 2. a cover; 3. a cover board placing cabin; 31. a limit frame; 4. a plug cover; 5. a push rod assembly; 51. the pushing block; 52. a suction cup; 521. a center portion; 522. an elastic connection part; 523. an edge ring; 53. a mounting rod; 54. a piston rod; 6. arc side lobes; 7. an elastic member; 80. a push ring; 81. a guide block; 82. a hook rod.
Detailed Description
In order to make the technical scheme of the present utility model better understood by those skilled in the art, the present utility model will be further described in detail with reference to the accompanying drawings.
As shown in fig. 1-3, a device for automatic sealing and tearing of an amplification instrument comprises a body, a sample cell is arranged on an upper platform of the body, a test tube die table 1 is arranged in the sample cell, a sealing cover 2 is rotationally arranged in the sample cell, the sealing cover 2 is used for covering the sample cell so as to enable the test tube die table 1 to be in a sealed space, a cover plate placing cabin 3 is arranged on the sealing cover 2, a plug cover 4 is arranged on the cover plate placing cabin 3, a push rod assembly 5 which is arranged in a rectangular array is arranged on the plug cover 4, the push rod assembly 5 comprises a push block 51, and a sucker 52 is arranged on one side of the push block 51 towards the test tube die table 1.
The number of the ejector rod assemblies 5 is the same as that of the test tube holes of the test tube bench 1.
Furthermore, the cover plate placing cabin 3 is provided with a limit frame 31 towards one end of the sample cell, and the end of the pushing block 51 is flush with one side of the limit frame 31 towards the cover plate placing cabin 3. During operation, it is necessary to open the stopper cap 4 and then place the cover plate in the cover plate placing compartment 3 and to stay in the retention frame 31. The test tube is a 96-well plate with the volume of 0.2ml, and is directly clamped in the concave of the table top of the sample cell.
According to the technology, the ejector rod assembly 5 is communicated with the air cavity in the plug cover 4, and the cover plate placed in the cover plate placing cabin 3 is covered by the ejector rod assembly 5 and the test tube by pressurizing, so that the sealing film is realized; or the cover plate is sucked to the suction cup 52 under negative pressure to effect film tearing.
As a further embodiment of the present utility model, the ejector rod assembly 5 includes a mounting rod 53, where the mounting rod 53 is screwed on the plug cover 4 and is communicated with the air cavity inside the plug cover 4;
the ejector rod assembly 5 further comprises a piston rod 54, wherein the piston rod 54 is movably arranged in the mounting rod 53 and is in threaded fit with the pushing block 51.
Further, the end of the piston rod 54 is provided with a one-way valve formed by stacking a plurality of arc-shaped side flaps 6, and the outer arc top of the one-way valve faces to one side of the air cavity.
Further, an elastic member 7 is provided between the mounting rod 53 and the piston rod 54.
Specifically, in the embodiment, due to the arrangement of the arc-shaped side lobes 6, a one-way valve is formed, and is in a closed state under the supercharging device, and in an open state under the negative pressure state, so that the piston rod 54 is communicated with the air cavity.
Under the pressure, the plurality of arc-shaped side flaps 6 are pressed, laminated, extruded and deformed to form a seal, and the piston rod 54 (based on the position shown in fig. 2) moves down at this time, and the sealing cover is ejected from the sealing cover plate and is clamped on the test tube, so that the sealing film is realized. When the air pressure is removed, the piston rod 54 is returned by the elastic member 7.
In the embodiment, the elastic member 7 may be a known elastic material such as an elastic metal plate, a spring, or the like.
As a further embodiment of the present utility model, the suction cup 52 includes a central portion 521, an elastic connection portion 522 (elastic silicone web) and an edge ring 523, the elastic connection portion 522 being for connecting the central portion 521 with the edge ring 523;
the pushing block 51 is movably provided with a pushing ring 80 moving along the vertical direction, the pushing ring 80 is connected with the edge ring 523, and the sucker 52 is switched between the following two modes:
in the first form, the edge ring 523 (rubber ring) extends to the outer side of the end surface of the pushing block 51, so that the sucker 52 is in a horn shape;
in the second configuration, the edge ring 523 is at the same level as the central portion 521 (rubber block) and is located in the push block 51.
Further, a plurality of guide blocks 81 are slidably arranged in a circumferential array in the pushing block 51, and a hook rod 82 is rotatably arranged between the guide blocks 81 and the push ring 80
Further, a bump is provided in the pushing block 51, and the bump is rotatably connected to the hook rod 82 and is disposed near the push ring 80.
Specifically, in the negative pressure state, gas enters the pushing block 51 from the center 521 and the piston rod 54 to the air cavity finally, and at this time, the plurality of arc-shaped side flaps 6 are in an open state; under negative pressure, the guide sliding block 81 moves along the position shown in fig. 3, and after moving upwards, the hook rod 82 is pulled to be synchronous, and the push ring 80 moves downwards by taking the convex point as a fulcrum, so that the edge ring 523 is positioned to abut against the outer side of the end face of the push block 51 to form a sucker, namely, a first form, the edge ring 523 is attached to the sealing cover, and the sealing cover 2 is opened, so that the sealing cover is separated, and the film is torn.
While certain exemplary embodiments of the present utility model have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that modifications may be made to the described embodiments in various different ways without departing from the spirit and scope of the utility model. Accordingly, the drawings and description are to be regarded as illustrative in nature and not as restrictive of the scope of the utility model, which is defined by the appended claims.
Claims (9)
1. Be applied to automatic sealing membrane of amplification instrument and device of dyestripping, including the body, offered the sample cell on its upper table, be provided with test tube die table (1) in the sample cell, the sample cell internal rotation is provided with closing cap (2), closing cap (2) are used for covering the sample cell so that test tube die table (1) are in sealed space, a serial communication port, be provided with apron on closing cap (2) and place cabin (3), be provided with on apron place cabin (3) cock cap (4), be provided with ejector pin subassembly (5) that are rectangular array and arrange on cock cap (4), ejector pin subassembly (5) are including supporting push block (51), support push block (51) orientation test tube die table (1) one side is provided with sucking disc (52).
2. The device for automatic sealing and tearing of amplification instrument according to claim 1, wherein the number of the ejector rod assembly (5) is consistent with that of the test tube holes of the test tube die table (1).
3. The device for automatic sealing and tearing of amplification instrument according to claim 1, wherein a limiting frame (31) is arranged at one end of the cover plate placing cabin (3) towards the sample cell, and the end of the pushing block (51) is flush with one side of the limiting frame (31) towards the cover plate placing cabin (3).
4. The device for automatic sealing and tearing of amplification instrument according to claim 1, wherein the ejector rod assembly (5) comprises a mounting rod (53), and the mounting rod (53) is connected to the plug cover (4) in a threaded manner and is communicated with an air cavity inside the plug cover (4);
the ejector rod assembly (5) further comprises a piston rod (54), and the piston rod (54) is movably arranged in the mounting rod (53) and is in threaded assembly with the pushing block (51).
5. The device for automatic sealing and tearing of an amplifying instrument according to claim 4, wherein the end of the piston rod (54) is provided with a one-way valve formed by stacking a plurality of arc-shaped side flaps (6), and the outer arc top of the one-way valve faces to one side of the air cavity.
6. The device for automatic sealing and tearing of an amplifying instrument according to claim 5, wherein an elastic member (7) is arranged between the mounting rod (53) and the piston rod (54).
7. The device for automatic sealing and tearing of amplification instruments according to claim 1, characterized in that said suction cup (52) comprises a central portion (521), an elastic connection portion (522) and an edge ring (523), said elastic connection portion (522) being adapted to connect said central portion (521) with said edge ring (523);
push rings (80) moving along the vertical direction are movably arranged in the push blocks (51), the push rings (80) are connected with the edge rings (523), and the sucker (52) is switched between the following two modes:
in a first mode, the edge ring (523) extends to the outer side of the end face of the pushing block (51) so that the sucker (52) is in a horn shape;
in a second configuration, the edge ring (523) is level with the central portion (521) and is located inside the thrust block (51).
8. The device for automatic sealing and tearing of amplification instrument according to claim 7, wherein a plurality of guide blocks (81) are slidably arranged in a circumferential array, and a hook rod (82) is rotatably arranged between the guide blocks (81) and the push ring (80).
9. The device for automatic sealing and tearing of amplification instrument according to claim 8, wherein a bump is arranged in the pushing block (51), and the bump is rotatably connected with the hook rod (82) and is arranged close to the push ring (80).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320462573.0U CN219770231U (en) | 2023-03-13 | 2023-03-13 | Be applied to automatic sealing membrane and dyestripping's of amplification instrument device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320462573.0U CN219770231U (en) | 2023-03-13 | 2023-03-13 | Be applied to automatic sealing membrane and dyestripping's of amplification instrument device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219770231U true CN219770231U (en) | 2023-09-29 |
Family
ID=88130447
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320462573.0U Active CN219770231U (en) | 2023-03-13 | 2023-03-13 | Be applied to automatic sealing membrane and dyestripping's of amplification instrument device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219770231U (en) |
-
2023
- 2023-03-13 CN CN202320462573.0U patent/CN219770231U/en active Active
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