CN219653030U - Cell sorting nanometer magnetic bead reagent preparation device - Google Patents

Abstract

The utility model discloses a preparation device for a cell sorting nanometer magnetic bead reagent, which comprises a preparation box, wherein a reagent tray is rotatably arranged in the preparation box, a plurality of reagent grooves are uniformly distributed in the reagent tray, a movable plate is rotatably arranged in the preparation box, a rotating plate is rotatably arranged in the preparation box, one end of the rotating plate is fixedly provided with a rotating block, the rotating block is movably contacted with the inner wall of the movable plate, a connecting column is fixedly arranged at the center of the bottom of the reagent tray, the bottom of the connecting column is fixedly connected with an arc plate, one end bottom of the arc plate, far away from the connecting column, is fixedly connected with a cylinder, and is movably contacted with the inner wall of the movable plate.

Description

Cell sorting nanometer magnetic bead reagent preparation device

Technical Field

The utility model relates to the technical field of nanometer magnetic bead reagents, in particular to a preparation device of a cell sorting nanometer magnetic bead reagent.

Background

Flow cell sorting is a modern cell analysis technology for qualitatively or quantitatively detecting physical, physiological, biochemical, immune, genetic, molecular biological properties, functional states and the like of cells (or particles), wherein a cell is separated from a multicellular sample, and a nano magnetic bead reagent is an essential reagent in the cell sorting process and is used for separating the cells by utilizing the characteristics of autoimmune magnetic beads;

when the nano magnetic bead reagent is prepared, the reagent raw materials are generally mixed and stirred, and are generally processed by manual shaking, so that the labor force of personnel is increased, the reagent raw materials are not easy to be fully mixed, the efficiency of cell sorting is reduced, the reagent in the hand is easy to fall off by manual operation, the waste and pollution of the whole reagent are caused, and the inventor proposes a device for preparing the nano magnetic bead reagent for cell sorting, which is used for solving the problems.

Disclosure of Invention

The method aims at solving the problems that reagent raw materials are not easy to be fully mixed and waste and pollution of the whole reagent are easy to be caused when the reagent raw materials are mixed and stirred manually; the utility model aims to provide a device for preparing a cell sorting nano magnetic bead reagent.

In order to solve the technical problems, the utility model adopts the following technical scheme: the utility model provides a nanometer magnetic bead reagent preparation facilities is selected separately to cell, includes the preparation case, the inside reagent dish that rotates of preparation case, the inside evenly distributed of reagent dish has a plurality of reagent groove, the fly leaf is installed in the inside rotation of preparation case, the inside rotation of preparation case is installed the fly leaf, the one end fixed mounting of fly leaf has the turning block, and turning block and fly leaf inner wall movable contact, reagent dish bottom central part department fixed mounting has the spliced pole, spliced pole bottom fixedly connected with arc, the one end bottom fixed connection cylinder of spliced pole is kept away from to the arc, and cylinder and fly leaf inner wall movable contact.

Preferably, the motor is fixedly installed in the preparation box, and the rotating end of the motor is fixedly connected with one end, far away from the rotating block, of the rotating plate.

Preferably, the preparation case is inside to rotate installs circular piece, the top fixedly connected with fixed block of circular piece, and fixed block and fly leaf keep away from the one end fixed connection of motor.

Preferably, a box cover is rotatably arranged on one side of the preparation box, and a handle is fixedly arranged on one side of the box cover.

Preferably, a plurality of storage tanks are uniformly distributed in the preparation box, and the storage tanks are positioned on two sides in the preparation box.

Preferably, one side of the reagent disk is rotatably provided with a reagent cover, a plurality of rubber covers are uniformly distributed in the reagent cover, the rubber covers respectively correspond to the reagent grooves, one side of the reagent cover is fixedly provided with a pull block, and the inside of the reagent grooves is fixedly provided with a rubber ring.

Preferably, a circle of limiting block is fixedly arranged on the outer side of the reagent disk, and the limiting block is in sliding connection with the preparation box.

Preferably, four fixing bases are fixedly arranged at the bottom of the preparation box, and the fixing bases are respectively positioned at the periphery of the bottom of the preparation box.

Compared with the prior art, the utility model has the beneficial effects that:

1. according to the utility model, the motor drives the rotating plate to rotate, the rotating plate drives the rotating block to rotate in the movable plate, the movable plate is in fan-shaped movement, the movable plate drives the arc plate to simultaneously perform fan-shaped movement through the cylinder, the connecting column drives the reagent disk to rotate back and forth, and reagent pipes in the reagent tank are fully mixed and stirred;

2. the utility model is provided with the rubber cover and the rubber ring, the rubber ring increases the friction force with the reagent tube, the rubber cover starts the fixing function to the top of the reagent tube, and the safety of the reagent tube is increased when the reagent tube rotates.

Drawings

In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of the overall structure of the present utility model.

FIG. 2 is a schematic view of the whole cross-sectional structure of the present utility model.

FIG. 3 is a schematic view of the whole cross-sectional structure of the present utility model.

Fig. 4 is an enlarged schematic view of the structure a in fig. 2 according to the present utility model.

Fig. 5 is an enlarged schematic view of the structure B in fig. 3 according to the present utility model.

In the figure: 1. preparing a box; 2. a box cover; 3. a handle; 4. a reservoir; 5. a reagent tray; 6. a reagent tank; 7. a fixed base; 8. a reagent cover; 9. pulling blocks; 10. a rubber cover; 11. a rubber ring; 12. a movable plate; 13. a rotating plate; 14. a rotating block; 15. a motor; 16. a limiting block; 17. a connecting column; 18. an arc-shaped plate; 19. a cylinder; 20. a circular block; 21. and a fixed block.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Examples: as shown in fig. 1-5, the utility model provides a preparation device for cell sorting nano magnetic bead reagent, which comprises a preparation box 1, wherein a reagent tray 5 is rotatably arranged in the preparation box 1, a plurality of reagent grooves 6 are uniformly distributed in the reagent tray 5, a movable plate 12 is rotatably arranged in the preparation box 1, a rotating plate 13 is rotatably arranged in the preparation box 1, one end of the rotating plate 13 is fixedly provided with a rotating block 14, the rotating block 14 is movably contacted with the inner wall of the movable plate 12, a connecting column 17 is fixedly arranged at the center of the bottom of the reagent tray 5, an arc-shaped plate 18 is fixedly connected with the bottom of the connecting column 17, one end bottom of the arc-shaped plate 18, which is far away from the connecting column 17, is fixedly connected with a cylinder 19, and the cylinder 19 is movably contacted with the inner wall of the movable plate 12.

The motor 15 is fixedly installed in the preparation box 1, and the rotating end of the motor 15 is fixedly connected with one end, far away from the rotating block 14, of the rotating plate 13.

Through adopting above-mentioned technical scheme, motor 15 drives rotor 13 and rotates, and rotor 13 drives rotor 14 at the inside rotation of fly leaf 12, and fan-shaped motion is done fly leaf 12, and fly leaf 12 drives arc 18 through cylinder 19 and is fan-shaped motion simultaneously, makes its spliced pole 17 drive reagent dish 5 round trip rotation, carries out intensive mixing stirring with the inside reagent pipe of reagent groove 6.

The preparation case 1 is internally provided with a round block 20 in a rotating way, the top end of the round block 20 is fixedly connected with a fixed block 21, and the fixed block 21 is fixedly connected with one end, far away from the motor 15, of the movable plate 12.

By adopting the technical scheme, the circular block 20 has the function of starting rotation limit on the movable plate 12, so that the movable plate 12 always takes the circular block 20 as a supporting point to perform fan-shaped movement.

The preparation case is characterized in that a case cover 2 is rotatably arranged on one side of the preparation case 1, and a handle 3 is fixedly arranged on one side of the case cover 2.

Through adopting above-mentioned technical scheme, rotate lid 2 through handle 3, lid 2 and preparation case 1 form the relative confined space, start the guard action to the inside reagent pipe of preparation case 1.

The preparation box 1 is internally and evenly provided with a plurality of storage tanks 4, and the storage tanks 4 are positioned at two sides of the interior of the preparation box 1.

Through adopting above-mentioned technical scheme, conveniently take reagent pipe.

One side of the reagent disk 5 is rotatably provided with a reagent cover 8, a plurality of rubber covers 10 are uniformly distributed in the reagent cover 8, the rubber covers 10 respectively correspond to the reagent grooves 6, one side of the reagent cover 8 is fixedly provided with a pull block 9, and a rubber ring 11 is fixedly arranged in the reagent grooves 6.

By adopting the technical scheme, the friction force between the rubber ring 11 and the reagent tube is increased, the safety of the reagent tube is improved when the reagent cap 8 rotates to be in contact with the reagent disc 5, the rubber cap 10 just contacts with the top of the reagent tube, and the safety of the reagent tube is improved when the reagent tube rotates.

A circle of limiting block 16 is fixedly arranged on the outer side of the reagent disk 5, and the limiting block 16 is in sliding connection with the preparation box 1.

Through adopting above-mentioned technical scheme, when reagent dish 5 rotates, stopper 16 slides along the spout of seting up inside preparation case 1, starts spacing effect when rotating reagent dish 5, stability when the assurance reagent dish 5 rotates.

Four fixed bases 7 are fixedly arranged at the bottom of the preparation box 1, and the fixed bases 7 are respectively positioned at the periphery of the bottom of the preparation box 1.

Through adopting above-mentioned technical scheme, unable adjustment base 7 bottom is made with rubber, increases the frictional force of preparation equipment and workstation, ensures the stability of preparation equipment during operation.

Working principle: firstly, taking out a reagent tube from the inside of a storage tank 4, sequentially placing reagent raw materials into the reagent tube, placing the assembled reagent tube into a reagent tank 6 in a reagent disk 5, placing the reagent tube into the reagent tank 6 through a rubber ring 11, rotating a reagent cover 8 until the reagent tube contacts with the reagent disk 5 at the moment, enabling a rubber cover 10 of the reagent tube to contact with the top of the reagent tube, enabling the rubber cover 10 and the rubber ring 11 to fix the reagent tube, starting a motor 15, driving a rotating plate 13 to rotate by the motor 15, driving a rotating block 14 to rotate in a movable plate 12, enabling the movable plate 12 to make fan-shaped movement, driving a circular block 20 to rotate, enabling the movable plate 12 to always make fan-shaped movement by taking the circular block 20 as a supporting point, driving an arc plate 18 to make fan-shaped movement at the same time by a connecting column 17 to drive the reagent disk 5, and fully mixing and stirring the raw materials in the reagent tube.

It will be apparent to those skilled in the art that various modifications and variations can be made to the present utility model without departing from the spirit or scope of the utility model. Thus, it is intended that the present utility model also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.

Claims (8)

1. The utility model provides a nanometer magnetic bead reagent preparation facilities is selected separately to cell, includes preparation case (1), its characterized in that: the preparation case (1) inside rotates installs reagent dish (5), inside evenly distributed of reagent dish (5) has a plurality of reagent groove (6), movable plate (12) are installed in preparation case (1) inside rotation, rotation board (13) are installed in preparation case (1) inside rotation, the one end fixed mounting of rotation board (13) has rotating block (14), and rotating block (14) and movable plate (12) inner wall movable contact, reagent dish (5) bottom central authorities department fixed mounting has spliced pole (17), spliced pole (17) bottom fixedly connected with arc (18), one end bottom fixed connection cylinder (19) of spliced pole (17) are kept away from to arc (18), and cylinder (19) and movable plate (12) inner wall movable contact.

2. The device for preparing the cell sorting nano magnetic bead reagent according to claim 1, wherein a motor (15) is fixedly arranged in the preparation box (1), and the rotating end of the motor (15) is fixedly connected with one end, far away from the rotating block (14), of the rotating plate (13).

3. The device for preparing the cell sorting nano magnetic bead reagent according to claim 1, wherein a circular block (20) is rotatably arranged in the preparation box (1), a fixed block (21) is fixedly connected to the top end of the circular block (20), and the fixed block (21) is fixedly connected with one end, far away from the motor (15), of the movable plate (12).

4. The device for preparing the cell sorting nano magnetic bead reagent according to claim 1, wherein a box cover (2) is rotatably arranged on one side of the preparation box (1), and a handle (3) is fixedly arranged on one side of the box cover (2).

5. The device for preparing the cell sorting nano magnetic bead reagent according to claim 1, wherein a plurality of storage tanks (4) are uniformly distributed in the preparation box (1), and the storage tanks (4) are positioned on two sides in the preparation box (1).

6. The preparation device for the cell sorting nano magnetic bead reagent according to claim 1, wherein a reagent cover (8) is rotatably arranged on one side of the reagent disk (5), a plurality of rubber covers (10) are uniformly distributed in the reagent cover (8), the rubber covers (10) respectively correspond to the reagent grooves (6), a pull block (9) is fixedly arranged on one side of the reagent cover (8), and a rubber ring (11) is fixedly arranged in the reagent grooves (6).

7. The device for preparing the cell sorting nano magnetic bead reagent according to claim 1, wherein a circle of limiting block (16) is fixedly arranged on the outer side of the reagent disk (5), and the limiting block (16) is in sliding connection with the preparation box (1).

8. The device for preparing the cell sorting nano magnetic bead reagent according to claim 1, wherein four fixing bases (7) are fixedly arranged at the bottom of the preparation box (1), and the fixing bases (7) are respectively arranged at the periphery of the bottom of the preparation box (1).