CN211856623U - Automatic sample loading device of carousel - Google Patents

Abstract

The invention relates to a rotary table automatic sample loading device, which comprises a shell, a sampling device, a blending device and a rotary table arranged in the shell, wherein the sampling device is arranged in the shell; the rotary table is connected with a driving motor through a synchronous belt, the driving motor drives the rotary table to rotate through the synchronous belt, and a plurality of test tubes which are circumferentially arranged are arranged on the rotary table; the sampling device comprises a lifting device and a sampling needle, the lifting device drives the sampling needle to move up and down, and the sampling needle is arranged above the test tube; the mixing device comprises a test tube sleeve and a mixing motor, the turntable drives the test tube to the test tube sleeve, and the mixing motor drives the test tube sleeve and the test tube arranged on the test tube sleeve to rotate; the driving motor is connected with a motor encoder used for detecting the rotation angle of the rotary table. The device is through setting up a plurality of test tubes in the carousel week, carries out the sample loading work of test tube automatically to setting for the position through rotatory.

Description

Automatic sample loading device of carousel

Technical Field

The invention relates to a sample loading device, in particular to an automatic sample loading device with a rotary disc.

Background

At present, flow cytometry has multiple sample loading modes, single-tube manual sample loading, multi-tube and 32-hole and 96-hole manual sample loading, and for the manual sample loading mode, the defects are as follows: the operation process has more steps, and more instruments and equipment are operated; the manual operation has poor accuracy and is easy to make mistakes; the efficiency of detecting a sample at one time is low and the intermediate process is likely to be interrupted for something else. In short, this process is tedious, inefficient, and prone to error.

Disclosure of Invention

The invention provides an automatic sample loading device of a turntable, aiming at solving the problem of low efficiency caused by multi-tube manual sample loading.

The technical scheme adopted by the invention is as follows: an automatic sample loading device of a rotary table comprises a shell, the rotary table, a sampling device and a mixing device, wherein the shell is provided with a window, and the window can be opened and closed;

the rotary table is connected with a driving motor through a synchronous belt, the driving motor drives the rotary table to rotate through the synchronous belt, and a plurality of test tubes which are circumferentially arranged are arranged on the rotary table;

the sampling device comprises a lifting device and a sampling needle, the lifting device drives the sampling needle to move up and down, and the sampling needle is arranged above the test tube;

the mixing device comprises a test tube sleeve and a mixing motor, the turntable drives the test tubes to sequentially pass through the test tube sleeve and be arranged on the test tube sleeve, and the mixing motor drives the test tube sleeve and the test tubes arranged on the test tube sleeve to rotate;

the driving motor is connected with a motor encoder used for detecting the rotation angle of the rotary table.

Furthermore, a sliding module is arranged in the shell, the turntable is arranged on the sliding module, the sliding module comprises a sliding plate arranged on the guide sliding rail and a guide sliding rail arranged on a bottom plate of the shell, the sliding plate is connected with the guide sliding rail in a sliding manner, and the sliding plate enters and exits the shell through the guide sliding rail; the window sets up on the upper end panel of casing, a side opening of casing, the slide passes through the opening business turn over the casing, the carousel set up in on the slide.

Furthermore, a baffle is fixedly connected to one side of the sliding plate, a handle for drawing the sliding plate is arranged on the baffle, and the baffle is used for sealing the opening of the shell after the sliding plate is pushed into the shell.

Furthermore, a tension wheel for freely adjusting the tension of the synchronous belt is arranged on the synchronous belt.

Furthermore, sampling device still includes the belt cleaning device who is used for wasing the sampling needle, belt cleaning device sets up under the sampling needle belt cleaning device is last to be provided with the through-hole, elevating gear drives when the sampling needle descends, the sampling needle passes the through-hole gets into the test tube.

Furthermore, a limiting shaft is arranged on the lifting device, a limiting pipe sleeve is arranged on the sliding plate, the limiting shaft is vertically arranged downwards and ascends or descends along with the lifting device, the limiting pipe sleeve is a pipe body with an opening at the upper end, and the limiting shaft ascends or descends to enter or exit the limiting pipe sleeve.

Furthermore, a magnetic contact switch used for judging whether the push-pull moving module is in place or not is arranged in the shell, a contact block is arranged on the sliding plate, the magnetic contact switch is fixed in the shell, and when the sliding plate is pushed into the shell and the contact block is in contact with the magnetic contact switch, the magnetic contact switch is triggered.

Further, be provided with in the casing and be used for sweeping a yard discernment test tube position sweep a yard module, sweep a yard module including sweeping a yard machine and attached in bar code on the test tube, it is in to record on the bar code the test tube is in positional information on the carousel.

Further, the mixing device set up in week side of carousel, the mixing device includes motor support and lift plate, be provided with the lead screw motor on the motor support, the lift plate passes through threaded connection the lead screw of lead screw motor, the lead screw motor drives the lift plate removes along the lead screw still be provided with the mixing motor that is used for the mixing test tube on the lift plate, the mixing motor is connected the test tube cover that is used for placeeing the test tube, the test tube is arranged in the test tube cover, the test tube cover distance the distance at carousel center with the test tube is equivalent apart from the distance at carousel center.

The beneficial effects produced by the invention comprise: the working intensity of manpower is greatly reduced, and the flow cytometer is matched with the flow cytometer for use, so that the working efficiency of the flow cytometer is effectively improved; the turntable has high precision, automatic operation according to a certain time sequence and high efficiency; the rotating process of the turntable is controlled by a system, and particularly, unmanned operation is realized by the operation sequence, the blending time, the sample suction time and the like; fourthly, the automatic needle cleaning machine has the functions of automatic mixing, code sweeping and needle collision prevention. The whole turntable is automatically operated by a cell instrument host, so that the use is more convenient.

Drawings

FIG. 1 is a schematic view of the automatic loading device according to the present invention;

FIG. 2 is a schematic view showing the structure of the device of the present invention placed on a slide;

FIG. 3 is a schematic view of the structure of the base plate of the present invention;

FIG. 4 is a schematic structural view of the housing of the present invention;

FIG. 5 is a schematic view of the internal device of the present invention with the housing removed;

FIG. 6 is a schematic view of the internal device of the present invention with the side panels of the housing removed;

FIG. 7 is a schematic view of the apparatus of the present invention with the turntable removed;

FIG. 8 is a schematic view showing the structure of the mixing device for test tubes according to the present invention;

FIG. 9 is a schematic diagram of the structure of the test tube mixing device and the flow cytometer;

FIG. 10 is a schematic view of the construction of the friction block of the present invention;

in the figure, the device comprises a shell, a 101 window, a 102 opening, a 103 bottom plate, a 2 rotating disc, a 3 rotating disc base, a 4 guiding rail, a 5 buffering limiting block, a 6 sliding plate, a 7 lifting device, an 8 sampling needle, a 9 cleaning device, a 901 cleaning through hole, a 10 baffle, a 11 guide tube, a 12 synchronous belt, a 13 tensioning wheel, a 14 motor encoder, a 15 limiting column, a 16 limiting pipe sleeve, a 17 drag chain, an 18 magnetic contact switch, a 19 touch block, a 20 driving motor, a 21 mixing device, a 22 lifting plate, a 23 screw rod, a 24 screw rod nut, a 25 linear bearing, a 26 polished rod, a 27 first through hole, a 28 through hole, a second through hole, a 29 third through hole, a 30 screw rod motor, a 31 mixing motor, a 32 friction block, a 33 shaft sleeve, a 34, an arc concave surface, a 35 motor bracket.

Detailed Description

The present invention is explained in further detail below with reference to the drawings and the specific embodiments, but it should be understood that the scope of the present invention is not limited to the specific embodiments.

As shown in fig. 1-7, the automatic sample loading device of the present invention includes a housing 1, a turntable 2 with a plurality of test tubes 11, a sampling device for sampling, a sliding module for pushing and pulling the turntable 2, a blending module for blending liquid in the test tubes 11, and a code scanning module for identifying positions of the test tubes 11, wherein an openable window 101 is provided on an upper end surface of the housing 1 through a hinge, a side opening 102 of the housing 1, a guide rail 4 is provided on a bottom plate 103 of the housing 1, the sliding module includes a guide rail 4 and a sliding plate 6, the sliding plate 6 is slidably connected to the guide rail 4, the sliding plate 6 moves along the guide rail 4, the guide rail 4 leads to the opening 102 of the housing 1, a baffle 10 is fixedly connected to one side of the sliding plate 6, a handle for pulling and pulling the sliding plate 6 is provided on the baffle 10, the baffle 10 is used for closing the opening 102 of the housing 1 after the sliding, when the sliding plate 6 needs to be pulled out, the handle on the baffle 10 is pulled, the sliding plate 6 moves out of the shell 1 along the guide sliding rail 4, and the buffer limiting block 5 is arranged at one end, close to the opening 102, of the guide sliding rail 4, so that the sliding plate 6 is prevented from being pulled and transited, and a limiting effect is achieved.

A turntable 2 seat and a turntable 2 are arranged on the sliding plate 6, 32 test tube 11 seats are arranged on the turntable 2 along the circumferential direction on the turntable 2 seat arranged on the turntable 2, used for placing the test tube 11, the rotary table 2 can be freely disassembled on the rotary table 2 seat, the rotary table 2 seat is connected with a driving motor 20, the driving motor 20 drives the rotary table 2 to rotate by driving the rotary table 2 seat to rotate, of course, the turntable 2 and the turntable 2 can be regarded as a whole, the driving motor 20 drives the turntable 2 to rotate through the synchronous belt 12, in order to adjust the tensioning degree of the synchronous belt 12, a tension pulley 13 is provided on the timing belt 12, and in order to monitor the rotation angle of the turntable 2, that is, the position of the test tube 11 is monitored, the motor encoder 14 is arranged on the turntable 2, the motor encoder 14 is connected with the driving motor 20, and the driving motor 20 starts or stops working according to the information of the motor encoder 14. The driving motor 20 is provided on the slide 6.

Keep away from one side of baffle 10 at slide 6 and be provided with sampling device, sampling device does not set up on slide 6, can't remove along with slide 6, sampling device includes elevating gear 7, sample needle 8 and belt cleaning device 9, the vertical setting of sample needle 8, the upper end sets up on elevating gear 7, belt cleaning device 9 includes clean through-hole 901, when elevating gear 7 handles highest position, clean through-hole setting is under sample needle 8, when elevating gear 7 drives sample needle 8 and descends, sample needle 8 passes in clean through-hole gets into test tube 11, obtain cleanly when passing clean through-hole. Cleaning device 9 does not follow elevating gear 7 up-and-down motion, still be provided with spacing post 15 on the elevating gear 7 in addition, elevating gear 7 is connected to the vertical setting of spacing post 15 and upper end, spacing post 15 is along with elevating gear 7 up-and-down motion, be provided with spacing pipe box on slide 6, spacing pipe box is upper end opening 102's body, when slide 6 pushes into to casing 1 in to the settlement position, spacing sleeve 16 is arranged in under spacing post 15, when elevating gear 7 drives spacing post 15 whereabouts, spacing post 15 inserts in the spacing pipe box, realize that slide 6 and casing 1's relative position is fixed, prevent that slide 6 from sliding in the sampling process.

The code scanning module comprises a code scanning machine and a bar code or a two-dimensional code arranged on the turntable 2, and corresponding position information (a turntable 21 position, a turntable 22 position … … turntable 232 position) is recorded on the bar code or the two-dimensional code, so that whether the position of the test tube 11 is consistent with the numbering position on the turntable 2 or not is automatically identified, and whether the system operates correctly or not is judged.

Be provided with the tow chain 17 that is used for accomodating the cable on slide 6, tow chain 17 is used for protecting the motor cable, and opto-coupler cable and encoder cable avoid the cable damaged in the push-and-pull motion.

A magnetic contact switch 18 is arranged at one end of the housing 1 away from the outlet, a touch block 19 is arranged on the sliding plate 6, and when the sliding plate 6 is pushed to a set position, the touch block 19 triggers the magnetic contact switch 18.

As shown in fig. 8-10, the blending device 21 is disposed on the periphery of the turntable 2, the blending device 21 includes a motor support 35 and a lifting plate 22, a lead screw motor 30 is disposed on the motor support 35, the lifting plate 22 is connected to the lead screw 23 of the lead screw motor 30 through a thread, the lead screw motor 30 drives the lifting plate 22 to move along the lead screw 23, a blending motor 31 for blending the test tubes 11 is further disposed on the lifting plate 22, the blending motor 31 is connected to the test tube 11 set for placing the test tubes 11, the test tubes 11 are placed in the test tube 11 set, and the distance from the center of the turntable 2 to the test tube 11 set is equivalent to the distance from the center of the turntable.

The blending device 21 specifically comprises a motor support 35 and a lifting plate 22, a screw motor 30 is installed on the motor support 35, the screw motor 30 is connected with the lifting plate 22 through a screw 23 and used for adjusting the position of the lifting plate 22 up and down, in order to enhance the stability of the connection of the lifting plate 22 and the screw 23, a first through hole 27 is formed in the lifting plate 22, a screw nut 24 is installed at the first through hole 27, the screw nut 24 is coaxial with the first through hole 27, the screw nut 24 is provided with an internal thread corresponding to the screw 23, one end of the screw 23 extends into the screw nut 24, and when the screw 23 motor drives the screw 23 to rotate, the screw nut 24 ascends or descends along the screw 23. The lead screw nut 24 is secured to the lift plate 22 by screws or bolts or other means.

In order to prevent the screw rod 23 from rotating selectively, the lifting plate 22 is driven to rotate, the polish rod 26 is arranged between the motor support 35 and the support plate, the second through hole 28 is arranged on the motor support 35, the linear bearing 25 is arranged in the second through hole 28, one end of the polish rod 26 is fixedly connected with the lifting plate 22, the other end of the polish rod 26 penetrates through the linear bearing 25, the polish rod 26 and the screw rod 23 are vertical rods, and further the polish rod 26 is detachably mounted on the lifting plate 22 through thread fit.

Install mixing motor 31 on lifting plate 22, mixing motor 31 sets up the one side that lifting plate 22 back to lead screw 23 motor, mixing motor 31 is high-speed direct current brushless motor, lead screw 23 motor is step motor, be provided with third through-hole 29 on lifting plate 22, mixing motor 31's pivot passes third through-hole 29 and connects test tube 11 cover, test tube 11 cover includes axle sleeve 33 and clutch blocks 32, axle sleeve 33 and clutch blocks 32 glue, axle sleeve 33 and mixing motor 31's pivot is fixed through the headless screw that radially sets up, clutch blocks 32 connects one side of test tube 11 and is provided with arc concave surface 34, be provided with heavy groove or the concave convex tissue that is used for increasing frictional force on arc concave surface 34, heavy groove distributes from concave surface center to outwards diverging, arc concave surface 34 shape is the same with test tube 11 bottom shape, clutch blocks 32 adopts the silica gel material of hardness 30A, after test tube 11 is placed on clutch blocks 32, through frictional force between the two, mixing motor 31 drives 11 covers of test tube and drives 11 rotations of test tube simultaneously, and then realizes the mixing.

Working method

1) Firstly, an automatic turntable 2 is installed in a matched flow cytometry tester, and the turntable 2 is initialized after the automatic turntable is electrified;

2) after the system self-checks, the system confirms that the parameters are set completely, and the turntable 2 is in a state to be tested

3) Pulling the handle on the window 101 until the top transparent window 101 is in the fully open position as shown in fig. 1, and removing the rotating disc 2

4) Placing the test tubes 11 to be tested with a maximum sampling amount of 32, then placing the turntable 2 back to the turntable base 3 (to ensure the relative positions of the turntable 2 and the turntable base 3, the arrow of the turntable 21 is aligned with the turntable base 3 and placed in the turntable 2) and pushing the slide plate 6 into the trigger electric shock switch as shown in figure 4, wherein 32 test tubes 11 on the turntable 2 process the positions 1-32 respectively,

5) the turntable 2 carries out a first test according to the test setting, the turntable 2 rotates the No. 1 position to a code scanning position through the stepping motor to start code scanning, identifies the number position of the corresponding test tube 11, checks whether the number positions recorded in the system are consistent, and carries out the next step if the number positions are consistent;

6) after the code scanning is finished, the position 1 is transferred to a blending position (namely the position of the blending device 21) to blend the reagents;

7) after the mixing is finished, the sample is transferred to the sample sucking position again, the sample sucking needle descends to the bottom of the test tube 11 to start the sample sucking test

After the test of test tube 11 No. 1 is finished, test tube 11 No. 2 is carried out, and the same code is swept first, then the sample is uniformly mixed, and the sample is sucked until all the test of 32 test tubes are finished.

The above description is only a preferred embodiment of the present invention, and the present invention is not limited to the content of the embodiment. It will be apparent to those skilled in the art that various changes and modifications can be made within the technical scope of the present invention, and any changes and modifications made are within the protective scope of the present invention.

Claims (9)

1. The utility model provides a carousel automatic loading appearance device which characterized in that: comprises a shell, a sampling device, a blending device and a turntable arranged in the shell;

the rotary table is connected with a driving motor through a synchronous belt, the driving motor drives the rotary table to rotate through the synchronous belt, and a plurality of test tubes which are circumferentially arranged are arranged on the rotary table;

the sampling device comprises a lifting device and a sampling needle, the lifting device drives the sampling needle to move up and down, and the sampling needle is arranged above the test tube;

the mixing device comprises a test tube sleeve and a mixing motor, the turntable drives the test tube to the test tube sleeve, and the mixing motor drives the test tube sleeve and the test tube arranged on the test tube sleeve to rotate;

the driving motor is connected with a motor encoder used for detecting the rotation angle of the rotary table.

2. The carousel automatic loading device of claim 1, characterized in that: a sliding module is arranged in the shell, the rotary table is arranged on the sliding module, the sliding module comprises a guide slide rail arranged on a bottom plate of the shell and a sliding plate arranged on the guide slide rail, the sliding plate is connected with the guide slide rail in a sliding manner, and the sliding plate enters and exits the shell through the guide slide rail; the upper end panel of the shell is provided with a window capable of being opened and closed, one side surface of the shell is provided with an opening, the sliding plate passes through the opening to enter and exit the shell, and the turntable is arranged on the sliding plate.

3. The carousel automatic loading device of claim 2, characterized in that: the sliding plate is characterized in that a baffle is fixedly connected to one side of the sliding plate, a handle used for drawing the sliding plate is arranged on the baffle, and the baffle is used for sealing an opening of the shell after the sliding plate is pushed into the shell.

4. The carousel automatic loading device of claim 1, characterized in that: and a tension wheel for freely adjusting the tension of the synchronous belt is arranged on the synchronous belt.

5. The carousel automatic loading device of claim 1, characterized in that: the sampling device further comprises a cleaning device used for cleaning the sampling needle, the cleaning device is arranged under the sampling needle, a through hole is formed in the cleaning device, the lifting device drives the sampling needle to descend, and the sampling needle penetrates through the through hole to enter the test tube.

6. The carousel automatic loading device of claim 2, characterized in that: the lifting device is provided with a limiting shaft, the sliding plate is provided with a limiting pipe sleeve, the limiting shaft is vertically arranged downwards and ascends or descends along with the lifting device, the limiting pipe sleeve is a pipe body with an opening at the upper end, and the limiting shaft ascends or descends to enter or exit the limiting pipe sleeve.

7. The carousel automatic loading device of claim 2, characterized in that: the push-pull moving module is characterized in that a magnetic contact switch used for judging whether the push-pull moving module is in place or not is arranged in the shell, a contact block is arranged on the sliding plate, the magnetic contact switch is fixed in the shell, and when the sliding plate is pushed into the shell and the contact block is in contact with the magnetic contact switch, the magnetic contact switch is triggered.

8. The carousel automatic loading device of claim 1, characterized in that: be provided with in the casing and be used for sweeping a yard discernment test tube position sweep a yard module, sweep a yard module including sweeping a yard machine and attached in the bar code of carousel, the positional information of test tube has been recorded on the bar code.

9. The carousel automatic loading device of claim 1, characterized in that: mixing device set up in week side of carousel, mixing device includes motor support and lifting plate, be provided with the lead screw motor on the motor support, the lifting plate passes through threaded connection the lead screw of lead screw motor, the lead screw motor drives the lifting plate removes along the lead screw still be provided with the mixing motor that is used for the mixing test tube on the lifting plate, the mixing motor is connected and is used for placeeing the test tube cover of test tube, the test tube is arranged in the test tube cover, the test tube cover distance the distance at carousel center with the distance of test tube apart from the carousel center is equal.

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