CN219559647U

CN219559647U - Microfluidic chip with pre-charging function

Info

Publication number: CN219559647U
Application number: CN202223169258.3U
Authority: CN
Inventors: 请求不公布姓名
Original assignee: Shenzhen Vitavitro Biotech Co ltd
Current assignee: Shenzhen Vitavitro Biotech Co ltd
Priority date: 2022-11-24
Filing date: 2022-11-24
Publication date: 2023-08-22
Anticipated expiration: 2032-11-24

Abstract

The utility model provides a microfluidic chip with a pre-filling function, which comprises a liquid path layer, wherein the liquid path layer is provided with at least two liquid path channels, the two liquid path channels are a main channel and an auxiliary channel respectively, an interception structure is arranged at the intersection of the two liquid path channels, and the interception structure intercepts a reagent, so that any one strand of reagent does not plug the channel where the other strand of reagent is located before converging. The beneficial effects of the utility model are as follows: when the microfluidic chip is pre-filled, the utility model avoids the generation of bubbles, thereby ensuring the safety of cells.

Description

Microfluidic chip with pre-charging function

Technical Field

The utility model relates to the technical field of cell processing, in particular to a microfluidic chip with a pre-charging function.

Background

For most microfluidic chips, prior to the actual operation, the interior of the microfluidic chip is usually required to be pre-filled with a certain or several specified liquids (which may also be referred to as reagents), namely: and (5) pre-filling the microfluidic chip. In the priming process, the reagent which arrives first in one channel can block the other channel at the junction of the two channels, so that bubbles are generated when the other strand of reagent advances from the other channel to the junction.

The presence of bubbles can disrupt the pressure distribution of the fluid control inside the microfluidic chip, and can also squeeze cells, even cause cell death, for microfluidic chips with cells. Since the generation of bubbles is quite random and the bubble removal process is difficult and difficult to standardize, it is desirable to avoid bubbles entirely during priming.

Disclosure of Invention

The utility model provides a microfluidic chip with a pre-filling function, which comprises a liquid path layer;

the liquid path channel is arranged on the liquid path layer, the reagent in the microfluidic chip flows through the liquid path channel, and the liquid path channel is provided with a main channel and an auxiliary channel which are crossed;

the interception structure is used for intercepting reagents in the liquid path channels and is arranged at the intersection of the two liquid path channels.

As a further improvement of the utility model, the interception structure is a dam body, and the dam body is provided with a groove which corresponds to the auxiliary channel.

As a further development of the utility model, the intersections are substantially rectangular.

As a further development of the utility model, the width of the intersection is wider than the width of the main channel.

As a further development of the utility model, the intersections exhibit a symmetrical arrangement.

As a further development of the utility model, the intersection is in the form of a smooth transition with the main channel.

As a further improvement of the utility model, the dam body has a shape of approximately letter n.

As a further improvement of the utility model, the dam is arranged at the bottom end of the intersection.

As a further improvement of the utility model, the height of the upper end of the dam body does not exceed the height of the main channel.

As a further improvement of the utility model, the height of the dam body is more than 50% of the height of the main channel, so that the dam body and the micro-fluidic chip are convenient to manufacture, and meanwhile, the interception effect is obviously realized.

As a further improvement of the utility model, the height of the dam body accounts for 85-95% of the height of the main channel, and the blocking effect of the dam body is inversely related to the size of the space, so that the space between the dam body and the upper wall of the channel is narrower as the dam body is higher, the blocking effect is better, but the passing efficiency of liquid is greatly reduced by the excessively high dam body, and various performances can be considered only at 85-95%, so that the passing efficiency is improved conveniently, the manufacturing is convenient, and the blocking effect is obvious.

As a further improvement of the utility model, the dam body and the liquid path channel of the micro-fluidic chip are formed in an integrated mode.

As a further improvement of the utility model, the dam body and the liquid path channel of the microfluidic chip are arranged at the bottom end of the intersection in an adhesive manner.

As a further improvement of the utility model, the dam body and the micro-fluidic chip are made of the same material.

As a further improvement of the utility model, the dam body is made of PDMS.

As a further improvement of the utility model, the dam body is provided with two supporting legs at one side, a groove is formed between the two supporting legs, and the groove is opposite to the position of the auxiliary channel in the microfluidic chip, so that the reagent 2 in the auxiliary channel can reach the groove.

As a further improvement of the utility model, the two supporting legs of the dam body are symmetrically arranged.

As a further improvement of the utility model, the width between the two legs of the dam exceeds half the width of the entire dam.

As a further improvement of the utility model, the micro-fluidic chip is also provided with an elastic film, and the elastic film is arranged on the end face of one side of the openings of the main channel and the auxiliary channel of the micro-fluidic chip.

As a further improvement of the utility model, a gap is arranged between the elastic film and the dam body.

As a further improvement of the utility model, the elastic film covers the intersections.

As a further improvement of the utility model, the elastic film covers all the main channels and the auxiliary channels.

As a further improvement of the present utility model, the elastic film covers the whole range of the microfluidic chip.

As a further improvement of the present utility model, the elastic film has a thickness of 50 to 250 micrometers, preferably 75 to 150 micrometers, because it is easy to manufacture, can have better elasticity, and has higher response sensitivity in control at 75 to 150 micrometers.

As a further improvement of the utility model, the elastic film is made of PDMS.

As a further improvement of the utility model, the microfluidic chip further comprises an air passage layer, the air passage layer is provided with an air passage channel connected with an external air pressure source, and the air passage layer and the elastic film are matched to form a pneumatic micro valve.

As a further development of the utility model, the gas circuit layer is provided with recesses in the region of the intersection, which recesses are connected to an external gas pressure source.

As a further improvement of the present utility model, the concave area of the air passage layer is not smaller than the area of the area at the intersection.

As a further improvement of the utility model, the shape of the recess of the air passage layer is consistent with the shape of the intersection.

As a further improvement of the utility model, the recess shape of the air passage layer is rectangular or circular.

As a further improvement of the utility model, the pneumatic micro valve position corresponds to the dam position.

As a further improvement of the utility model, when negative pressure is applied to the air passage in the air passage layer, the elastic film under the air passage is bent upwards, and contracts towards the air passage space direction in the air passage layer, so that the passage between the dam body and the elastic film is enlarged.

As a further improvement of the utility model, the number of the auxiliary channels is two, the number of the grooves is two, the two auxiliary channels are respectively positioned at the left side and the right side of the main channel, one groove corresponds to one auxiliary channel, and the other groove corresponds to the other auxiliary channel.

As a further improvement of the utility model, the number of the auxiliary channels is two, the two auxiliary channels are positioned on the same side of the main channel, and the grooves correspond to the two auxiliary channels.

As a further improvement of the utility model, the length of the groove is greater than 0% of the length of the dam, and the length of the groove is less than 100% of the length of the dam.

As a further improvement of the utility model, the length of the groove accounts for 20-80% of the length of the dam body.

As a further improvement of the utility model, the interception structure is a single-channel dam body, two single-channel dam bodies are respectively a first single-channel dam body and a second single-channel dam body, the first single-channel dam body is arranged on the auxiliary channel, and the second single-channel dam body is arranged on the main channel.

As a further improvement of the utility model, the single channel dam is substantially strip-shaped.

As a further improvement of the utility model, the height of the upper end of the first single-channel dam body does not exceed the height of the auxiliary channel, and the height of the upper end of the second single-channel dam body does not exceed the height of the main channel.

As a further improvement of the utility model, the height of the upper end of the first single-channel dam body is more than 50% of the height of the auxiliary channel, and the height of the upper end of the second single-channel dam body is more than 50% of the height of the main channel.

As a further improvement of the utility model, the height of the upper end of the first single-channel dam body accounts for 85-95% of the height of the auxiliary channel, and the height of the upper end of the second single-channel dam body accounts for 85-95% of the height of the main channel. Because the blocking effect of the dam is inversely related to the space, the higher the dam is, the narrower the space between the dam and the upper wall of the channel is, and the better the blocking effect is, so the height of the dam is preferably 85-95% of the height of the main channel.

As a further improvement of the utility model, the single-channel dam body and the micro-fluidic chip are made of the same material.

As a further improvement of the utility model, the single-channel dam body is made of PDMS.

As a further improvement of the utility model, the microfluidic chip further comprises an air passage layer, the air passage layer is provided with an air passage channel connected with an external air pressure source, the air passage layer and the elastic film are matched to form a pneumatic micro valve, the pneumatic micro valve comprises a third pneumatic micro valve and a fourth pneumatic micro valve, the third pneumatic micro valve corresponds to the position of the first single-channel dam, and the fourth pneumatic micro valve corresponds to the position of the second single-channel dam.

As a further improvement of the utility model, the interception structure is a dam body, the dam body is provided with a groove, and the groove corresponds to the auxiliary channel; the dam body is arranged at the bottom end of the intersection; the height of the upper end of the dam body does not exceed the height of the main channel, and the height of the dam body accounts for more than 50% of the height of the main channel; the height of the dam body accounts for 85-95% of the height of the main channel; the dam body and the liquid path channel of the microfluidic chip are of an integrated structure; two supporting legs are arranged on one side of the dam body, a groove is formed between the two supporting legs, and the groove corresponds to the position of the auxiliary channel in the microfluidic chip; the width between two supporting legs of the dam body exceeds half of the width of the whole dam body; the micro-fluidic chip is also provided with an elastic film which is arranged on the end face of one side of the openings of the main channel and the auxiliary channel of the micro-fluidic chip; a gap is formed between the elastic film and the dam body; the elastic film has a thickness of 50 to 250 micrometers; the thickness of the elastic film is 75-150 micrometers; the micro-fluidic chip further comprises an air passage layer, the air passage layer is provided with an air passage channel connected with an external air pressure source, and the air passage layer and the elastic film are matched to form a pneumatic micro valve; the air passage layer is provided with a concave part in a region corresponding to the crossing part; the pneumatic micro valve position corresponds to the dam body position; when negative pressure is applied to the air passage in the air passage layer, the elastic film under the air passage bends upwards, and contracts towards the air passage space direction in the air passage layer, so that the passage between the dam body and the elastic film is enlarged; the number of the auxiliary channels is two, the two auxiliary channels are respectively positioned at the left side and the right side of the main channel, one of the grooves corresponds to one auxiliary channel, and the other groove corresponds to the other auxiliary channel; or the number of the auxiliary channels is two, the two auxiliary channels are positioned on the same side of the main channel, and the grooves correspond to the two auxiliary channels; the length of the groove accounts for 20-80% of the length of the dam body; the interception structures are two single-channel dams, namely a first single-channel dam and a second single-channel dam, wherein the first single-channel dam is arranged on the auxiliary channel, and the second single-channel dam is arranged on the main channel; the single-channel dam body is approximately strip-shaped; the height of the upper end of the first single-channel dam body does not exceed the height of the auxiliary channel, the height of the upper end of the second single-channel dam body does not exceed the height of the main channel, the height of the upper end of the first single-channel dam body accounts for more than 50% of the height of the auxiliary channel, and the height of the upper end of the second single-channel dam body accounts for more than 50% of the height of the main channel; the height of the upper end of the first single-channel dam body accounts for 85-95% of the height of the auxiliary channel, and the height of the upper end of the second single-channel dam body accounts for 85-95% of the height of the main channel; the micro-fluidic chip further comprises an air passage layer, the air passage layer is provided with an air passage channel connected with an external air pressure source, the air passage layer and the elastic film are matched to form a pneumatic micro valve, the pneumatic micro valve comprises a third pneumatic micro valve and a fourth pneumatic micro valve, the third pneumatic micro valve corresponds to the position of the first single-channel dam, and the fourth pneumatic micro valve corresponds to the position of the second single-channel dam.

The beneficial effects of the utility model are as follows: the inventor researches and discovers that the gas mixing in the existing chip treatment process is not only brought by the filled reagent solution, but also can be formed in the collecting process when different reagent solutions are introduced into the chip.

Drawings

FIG. 1 is a schematic diagram of a liquid layer structure with a dam;

FIG. 2 is a schematic diagram of an embodiment of a liquid path layer with a dam;

FIG. 3 is a schematic view of another embodiment of a liquid path layer with a dam;

FIG. 4 is a schematic view of the elastic film structure;

FIG. 5 is a schematic diagram of the principle of a microfluidic chip;

FIG. 6 is a schematic diagram of a liquid path layer structure with a single channel dam.

Detailed Description

The utility model mainly aims to prevent bubbles from being generated when the microfluidic chip is precharged. The utility model discloses a priming method based on a microfluidic chip, wherein reagents introduced into the microfluidic chip comprise a reagent 1 flowing through a main channel 4 and a reagent 2 flowing through an auxiliary channel 5;

after the reagent 1 is input through the corresponding input port, the reagent flows in a main channel 4 in the microfluidic chip; after the reagent 2 is input through the corresponding input port, the reagent flows in the auxiliary channel 5 in the microfluidic chip;

an intersection is formed between the main channel 4 and the auxiliary channel 5, so that reagents of the main channel 4 and the auxiliary channel 5 can meet at the intersection;

the pre-filling method further comprises a converging step, wherein in the converging step, the reagent is intercepted through the intercepting structure, so that the fact that any one strand of reagent has certain cross overlapping and is not blocked on a channel where the other strand of reagent is located before converging is achieved.

As an embodiment of the pre-filling method, the intercepting structure is a dam 2, in the step of converging, by controlling the flow of the reagent in the main channel 4 and the auxiliary channel 5, the reagent 2 input by the auxiliary channel 5 reaches the intersection first, the dam 2 is arranged at the intersection, and the reagent 2 is intercepted first by the dam 2, so that the reagent 2 cannot cross the dam 2;

subsequently, the reagent 1 fed in by the main channel 4 also reaches the intersection;

after passing over the dam 2 at the intersection, the reagent 1 contacts the reagent 2 and merges to form a synthetic flow, and the synthetic flow continues to flow downstream.

The intersections are substantially rectangular and the width of the intersections is wider than the width of the main channel 4, the intersections exhibiting a symmetrical arrangement. The intersection is in the form of a smooth transition with the main channel 4.

As shown in fig. 1, the dam body 2 is provided with a groove 3, the dam body 2 is approximately in the shape of letter n, and the dam body 2 is arranged at the bottom end of the intersection.

The height of the upper end of the dam body 2 does not exceed the height of the main channel 4, for example, the height of the dam body 2 accounts for more than 50% of the height of the main channel 4, so that the manufacturing of the dam body and the micro-fluidic chip is facilitated, and meanwhile, the interception effect is obviously realized. Preferably, the height of the dam 2 is 85-95% of the height of the main channel 4, and the higher the dam 2 is, the narrower the space between the dam 2 and the upper wall of the channel is, because the interception effect of the dam 2 is inversely related to the space, and the better the interception effect is, so the height of the dam 2 is preferably 85-95% of the height of the main channel 4.

The dam body 2 and the liquid path channel of the microfluidic chip can be an integrated structure formed in an integrated mode. Alternatively, the dam 2 and the liquid path channel of the microfluidic chip are arranged at the intersection in an adhesive manner, and are preferably adhered to the bottom end.

The dam body 2 and the micro-fluidic chip can be made of the same material, and if the dam body 2 and the micro-fluidic chip are made of the same material, the process difficulty is smaller and the cost is lower. The microfluidic chip in the utility model can be realized by adopting various materials commonly used for microfluidic chips. Or the dam body 2 is made of PDMS. The chinese name of PDMS is polydimethylsiloxane. The PDMS microstructure is formed on the surface of the microfluidic chip by a molding method, the turnover accuracy is high, the nano (nm) level can be achieved, and the control accuracy of the microfluidic chip can be greatly improved.

The dam body 2 has two stabilizer blades on one side, and recess 3 formed between two stabilizer blades, and recess 3 just faces the position of auxiliary channel 5 in the micro-fluidic chip to make reagent 2 in the auxiliary channel 5 reach recess 3.

The two legs of the dam body 2 are symmetrically arranged, and the width between the legs, namely the width of the groove 3, is not too narrow, so that unnecessary resistance can be generated. Thus, the width of the groove 3 maintains the width of the auxiliary channel 5. The width of the left support leg corresponds to the path length of the reagent 1 passing through the top end of the dam body 2, and the width of the right support leg corresponds to the path length of the solution passing through the top end of the dam body 2 after confluence, and the width of the support leg is not excessively large due to resistance, so that the width between the two support legs of the dam body 2 exceeds half of the width of the whole dam body 2.

The micro-fluidic chip is also provided with an elastic film 7, the elastic film 7 is arranged on the end surfaces of the openings of the main channel 4 and the auxiliary channel 5 of the micro-fluidic chip, and a gap is reserved between the elastic film 7 and the dam body 2.

The elastic membrane 7 may cover only the intersections, or the elastic membrane 7 covers all of the main channels 4 and the sub channels 5; alternatively, the elastic film 7 covers the entire range of the microfluidic chip.

The thickness of the elastic film 7 is 50 to 250 micrometers, preferably 75 to 150 micrometers, and the elastic film 7 is made of PDMS.

The micro-fluidic chip further comprises an air passage layer 6, the air passage layer 6 is provided with an air passage channel connected with an external air pressure source, the air passage layer 6 and the elastic film 7 are matched to form a pneumatic micro-valve, the pneumatic micro-valve comprises a first pneumatic micro-valve and a second pneumatic micro-valve, the dam body 2 is in the shape of letter n, the dam body 2 is provided with two support legs, a groove 3 is formed between the two support legs, the groove 3 corresponds to the position of the auxiliary channel 5 in the micro-fluidic chip, the two support legs are a first support leg 31 and a second support leg 32 respectively, the first support leg 31 is positioned on one inflow side of the reagent 1, the second support leg 32 is positioned on one confluence outflow side of the reagent 1 and the reagent 2, the first pneumatic micro-valve controls the main channel 4, and the first pneumatic micro-valve only covers part of the first support leg 31; the second pneumatic micro valve controls the auxiliary channel 5, the second pneumatic micro valve covers part of the groove 3, the second pneumatic micro valve does not intercept the whole auxiliary channel 5 and the cross section of the groove 3, and the reagent 1 is prevented from being blocked when the second pneumatic micro valve is closed; to spatially accommodate the two pneumatic microvalves, the first leg 31 is elongated; in the confluence step, introducing the reagent 1 into the main channel 4, applying positive pressure to the first pneumatic micro valve when the reagent 1 in the main channel 4 advances to the dam body 2, closing the first pneumatic micro valve, and enabling the elastic film 7 of the first pneumatic micro valve to be in downward contact with the dam body 2 so as to close the main channel 4;

introducing the reagent 2 into the auxiliary channel 5, applying positive pressure to the second pneumatic micro valve when the reagent 2 advances to the dam 2, closing the second pneumatic micro valve, and stopping the driving pressure of the reagent 2;

applying negative pressure to the first pneumatic micro valve, wherein the elastic film 7 of the first pneumatic micro valve protrudes upwards (or removing the positive pressure of the first pneumatic micro valve, and the elastic film 7 of the first pneumatic micro valve restores to a plane state), driving the reagent 1 to continuously advance beyond the dam body 2, removing the positive pressure of the second pneumatic micro valve when the reagent 1 advances to the second pneumatic micro valve (or applying negative pressure to the second pneumatic micro valve, and protruding the elastic film 7 of the second pneumatic micro valve upwards), and completing confluence with the reagent 1 when the reagent passes above the reagent 2;

and when the converged reagent passes through the dam body 2 and enters the waste liquid channel, the negative pressure of the first pneumatic micro valve is removed, the waste liquid channel is continuously filled, and the pre-filling is completed.

The gas path layer 6 is provided with recesses in the corresponding areas of the intersections, through which recesses an external gas pressure source can be communicated. The concave area of the air passage layer 6 is not smaller than the area of the area at the intersection. The concave shape of the air passage layer 6 is substantially identical to the shape of the intersection. The recess of the gas path layer 6 is preferably rectangular or circular in shape.

The pneumatic micro valve is positioned corresponding to the position of the dam body 2, when negative pressure is applied to the air passage in the air passage layer 6, the elastic film 7 under the air passage is bent upwards, and contracts towards the air passage space direction in the air passage layer 6, so that the passage between the dam body 2 and the elastic film 7 is enlarged.

By controlling the flow of the reagent in the main channel 4 and the auxiliary channel 5, the reagent 2 input by the auxiliary channel 5 reaches the intersection firstly, the dam body 2 is arranged at the intersection, the reagent 2 is intercepted firstly by the groove 3 of the dam body 2, and the reagent 2 cannot cross the groove 3 of the dam body 2; subsequently, the reagent 1 fed in by the main channel 4 also reaches the intersection; the reagent 1 contacts with the reagent 2 in the groove 3 to complete confluence when passing over the groove 3, then the two reagents are combined to form a synthetic flow, and the synthetic flow finally passes over the dam body 2 to continue to flow downwards.

The length L1 of the groove 3 is greater than 0% of the length L2 of the dam 2, and the length L1 of the groove 3 is less than 100% of the length L2 of the dam 2; preferably, the length L1 of the groove 3 accounts for 20-80% of the length L2 of the dam 2.

As an embodiment of the pre-filling method, two auxiliary channels 5 are provided, the dam body 2 is provided with two grooves 3, the two auxiliary channels 5 are respectively positioned at the left side and the right side of the main channel 4, one groove 3 corresponds to one auxiliary channel 5, the other groove 3 corresponds to the other auxiliary channel 5, the flow of the reagent in the main channel 4 and the two auxiliary channels 5 is controlled, so that the reagent 2 input by the two auxiliary channels 5 reaches the intersection first, the intersection is provided with the dam body 2, the reagent 2 is intercepted first by the two grooves 3 of the dam body 2, and the reagent 2 cannot cross the two grooves 3 of the dam body 2;

the reagent 1 contacts with the reagent 2 in the two grooves 3 to complete confluence when passing over the two grooves 3, then the two reagents are combined to form a synthetic flow, and finally the synthetic flow passes over the dam body 2 to continue to flow downstream.

As an embodiment of the pre-filling method, the interception structures are two single-channel dams 17, namely a first single-channel dam and a second single-channel dam, the first single-channel dam is arranged on the auxiliary channel 5, the second single-channel dam is arranged on the main channel 4, and in the step of converging, the reagent 2 input by the auxiliary channel 5 is intercepted by the first single-channel dam before reaching the intersection by controlling the flow of the reagent in the main channel 4 and the auxiliary channel 5, so that the reagent 2 cannot cross the first single-channel dam;

the reagent 1 input by the main channel 4 is intercepted by the second single-channel dam before reaching the intersection, so that the reagent 2 cannot cross the second single-channel dam;

reagent 1 and reagent 2 are controlled to simultaneously cross the first single-channel dam and the second single-channel dam, so that reagent 1 and reagent 2 reach the intersection at the same time, and after the reagent 1 contacts with reagent 2, the reagent 1 and reagent 2 are combined to form a synthetic flow, and the synthetic flow continues to flow downwards.

The single-channel dam body is used for being arranged on the main channel 4 or the auxiliary channel 5 and used for intercepting. The dam 2 is intended to be placed at the intersection of two channels. The dam body 2 can be a physical structure for intercepting liquid (such as various reagents) or solid (such as cells to be processed) in a channel of the microfluidic chip, and as the dam body 2 is arranged in the channel, certain resistance or blocking is caused to the liquid or solid passing, the dam body 2 can be various shapes or other components or mechanisms with other relevant forms which can play the role of interception.

The micro-fluidic chip further comprises an air channel layer 6, the air channel layer 6 is provided with an air channel connected with an external air pressure source, the air channel layer 6 and the elastic film 7 are matched to form a pneumatic micro valve, the pneumatic micro valve comprises a third pneumatic micro valve and a fourth pneumatic micro valve, the third pneumatic micro valve corresponds to the first single-channel dam, the fourth pneumatic micro valve corresponds to the second single-channel dam, in the converging step, the reagent 1 is introduced into the main channel 4, positive pressure is applied to the fourth pneumatic micro valve when the reagent 1 advances to the front of the second single-channel dam, the fourth pneumatic micro valve is closed, and the elastic film 7 of the fourth pneumatic micro valve is downward contacted with the second single-channel dam, so that the main channel 4 is closed;

introducing the reagent 2 into the auxiliary channel 5, applying positive pressure to the third pneumatic micro valve when the reagent 2 advances to the front of the first single-channel dam, closing the third pneumatic micro valve, and enabling an elastic film 7 of the third pneumatic micro valve to be in downward contact with the first single-channel dam so as to close the auxiliary channel 5;

and removing positive pressure of the third pneumatic micro valve and the fourth pneumatic micro valve to enable the elastic film 7 of the third pneumatic micro valve to restore to the plane state and the elastic film 7 of the fourth pneumatic micro valve to restore to the plane state, or applying negative pressure to the third pneumatic micro valve and the fourth pneumatic micro valve to enable the elastic film 7 of the third pneumatic micro valve to bulge upwards and the elastic film 7 of the fourth pneumatic micro valve to bulge upwards, then controlling the reagent 1 and the reagent 2 to simultaneously pass through the first single channel dam body and the second single channel dam body, enabling the reagent 1 and the reagent 2 to simultaneously reach the intersection, enabling the reagent 1 and the reagent 2 to be combined together to form a synthetic flow after being contacted, and enabling the synthetic flow to continue to flow downwards to finish pre-charging.

The single channel dam 17 is generally elongated in shape.

The height of the upper end of the first single-channel dam body does not exceed the height of the auxiliary channel 5, and the height of the upper end of the second single-channel dam body does not exceed the height of the main channel 4; for example, the height of the upper end of the first single-channel dam body is more than 50% of the height of the auxiliary channel 5, and the height of the upper end of the second single-channel dam body is more than 50% of the height of the main channel 4; preferably, the upper end of the first single-channel dam body accounts for 85-95% of the height of the auxiliary channel 5, and the upper end of the second single-channel dam body accounts for 85-95% of the height of the main channel 4.

The material of the single-channel dam 17 is the same as that of the microfluidic chip, and preferably, the material of the single-channel dam 17 is PDMS.

The utility model also discloses a microfluidic chip with the pre-filling function, which comprises a liquid path layer 1;

the liquid path channel is arranged on the liquid path layer 1, and the reagent in the microfluidic chip flows through the liquid path channel, and the liquid path channel is provided with a main channel 4 and an auxiliary channel 5 which are crossed;

the interception structure is used for intercepting reagents in the liquid path channels and is arranged at the intersection of the two liquid path channels, so that the situation that any one strand of reagents have certain intersection overlapping and are not blocked on the channel where the other strand of reagents are located before converging is realized.

As an embodiment of the microfluidic chip, the interception structure is a dam body 2, the dam body 2 is provided with a groove 3, and the groove 3 corresponds to the auxiliary channel 5.

The dam body 2 takes the shape of approximate letter n, and the dam body 2 is arranged at the bottom end of the intersection.

The height of the upper end of the dam 2 does not exceed the height of the main channel 4, for example, the height of the dam 2 is more than 50% of the height of the main channel 4, preferably the height of the dam 2 is 85-95% of the height of the main channel 4.

The dam body 2 and the liquid path channel of the microfluidic chip can be an integrated structure formed in an integrated mode. Or the dam body and the liquid path channel of the microfluidic chip are arranged at the intersection in an adhesive mode, and are preferably adhered to the bottom end.

The material of the dam body 2 and the micro-fluidic chip can be the same, or the material of the dam body 2 is PDMS.

The dam body 2 has two stabilizer blades on one side, forms recess 3 between two stabilizer blades, and the position of recess 3 corresponding to auxiliary channel 5 in the micro-fluidic chip is in order to make the reagent 2 in auxiliary channel 5 reach recess 3.

The two supporting legs of the dam body 2 are symmetrically arranged, and the width between the two supporting legs of the dam body 2 exceeds half of the width of the whole dam body 2.

The microfluidic chip is also provided with an elastic film 7, the elastic film 7 is arranged on the end surfaces of the openings of the main channel 4 and the auxiliary channel 5 of the microfluidic chip, and a gap is reserved between the elastic film 7 and the dam body 2.

The micro-fluidic chip further comprises an air passage layer 6, the air passage layer 6 is provided with an air passage connected with an external air pressure source, and the air passage layer 6 and the elastic film 7 are matched to form a pneumatic micro valve.

As shown in fig. 2, as an embodiment of the liquid path layer 1, two auxiliary channels 5 are provided, two grooves 3 are provided, two auxiliary channels 5 are respectively located at the left and right sides of the main channel 4, one groove 3 corresponds to one auxiliary channel 5, and the other groove 3 corresponds to the other auxiliary channel 5.

As another embodiment of the liquid path layer 1, as shown in fig. 3, two auxiliary channels 5 are provided, two auxiliary channels 5 are located on the same side of the main channel 4, and the grooves 3 correspond to two auxiliary channels 5.

During operation, the reagent input by the auxiliary channel 5 stays at the groove 3, the reagent input by the main channel 4 contacts with the reagent in the groove 3 to complete confluence when passing through the upper part of the groove 3, then the two reagents are combined to form a synthetic flow, and finally the synthetic flow passes through the dam body 2 to continue to flow downstream.

As shown in fig. 4, the microfluidic chip further includes an elastic film 7, and since the height of the dam 2 does not exceed the height of the liquid path channel of the liquid path layer 1, a certain gap is formed between the elastic film 7 and the dam 2. When the elastic film 7 is provided on the upper side of the liquid path layer 1, the circulation of the reagent liquid in each liquid path channel in the liquid path layer 1 is not disturbed.

As shown in fig. 5, the microfluidic chip further includes an air path layer 6, where the air path layer 6 may be connected to an external controllable air pressure source so as to provide a certain pressure in the air path layer 6, for example, may provide positive pressure and negative pressure; the pneumatic micro valve is formed by matching the air passage layer 6 and the elastic film 7 and is used for controlling the opening and closing of each liquid passage of the liquid passage layer 1.

As another embodiment of the microfluidic chip, as shown in fig. 6, the interception structure is a single channel dam 17, where the single channel dam is a dam that can only implement the interception function on a single channel, such as a main channel or an auxiliary channel. The number of the single-channel dam bodies 17 is two, namely a first single-channel dam body and a second single-channel dam body, wherein the first single-channel dam body is arranged on the auxiliary channel 5, and the second single-channel dam body is arranged on the main channel 4.

The single channel dam 17 is generally elongated in shape.

The material of the single channel dam 17 is the same as that of the microfluidic chip, and preferably, the material of the single channel dam 17 is PDMS.

The micro-fluidic chip further comprises an air passage layer 6, the air passage layer 6 is provided with an air passage connected with an external air pressure source, the air passage layer 6 and the elastic film 7 are matched to form a pneumatic micro valve, the pneumatic micro valve comprises a third pneumatic micro valve and a fourth pneumatic micro valve, the third pneumatic micro valve corresponds to the first single-channel dam in position, and the fourth pneumatic micro valve corresponds to the second single-channel dam in position.

Principle of pneumatic microvalve: when negative pressure is applied to the air passage in the air passage layer 6, the elastic film 7 under the air passage is bent upwards and contracted towards the air passage space direction in the air passage layer 6, so that the passage between the dam 2 or the single-passage dam 17 of the liquid passage layer and the elastic film 7 is enlarged, and more fluid and cells convenient to process pass through; when positive pressure is injected into the air passage in the air passage layer 6, the elastic film 7 below the air passage bends downwards to extrude the liquid passage below the elastic film 7; when the positive pressure is removed, the elastic membrane 7 is restored, thereby realizing pneumatic micro valve control.

The utility model adopts the dam body 2 or the single-channel dam body 17 to stop the generation of bubbles when reagents are converged, thereby avoiding the interference of the bubbles on the cell treatment process.

When the microfluidic chip is pre-filled, the utility model avoids the generation of bubbles, thereby ensuring the safety of cells.

The foregoing is a further detailed description of the utility model in connection with the preferred embodiments, and it is not intended that the utility model be limited to the specific embodiments described. It will be apparent to those skilled in the art that several simple deductions or substitutions may be made without departing from the spirit of the utility model, and these should be considered to be within the scope of the utility model.

Claims

1. The utility model provides a micro-fluidic chip with precharge function which characterized in that: comprises a liquid path layer (1);

the liquid path channel is arranged on the liquid path layer (1), the reagent in the microfluidic chip flows through the liquid path channel, and the liquid path channel is provided with a main channel (4) and an auxiliary channel (5) which are crossed;

2. The microfluidic chip of claim 1, wherein: the interception structure is a dam body (2), the dam body (2) is provided with a groove (3), and the groove (3) corresponds to the auxiliary channel (5).

3. The microfluidic chip of claim 2, wherein: the dam body (2) is arranged at the bottom end of the intersection.

4. The microfluidic chip of claim 2, wherein: the height of the upper end of the dam body (2) does not exceed the height of the main channel (4), and the height of the dam body (2) accounts for more than 50% of the height of the main channel (4).

5. The microfluidic chip according to claim 4, wherein: the height of the dam body (2) accounts for 85-95% of the height of the main channel (4).

6. The microfluidic chip of claim 2, wherein: the dam body (2) and the liquid path channel of the micro-fluidic chip are of an integrated structure.

7. The microfluidic chip of claim 2, wherein: two supporting legs are arranged on one side of the dam body (2), a groove (3) is formed between the two supporting legs, and the groove (3) corresponds to the position of the auxiliary channel (5) in the microfluidic chip.

8. The microfluidic chip of claim 7, wherein: the width between two supporting legs of the dam body (2) exceeds half of the width of the whole dam body (2).

9. The microfluidic chip of claim 2, wherein: the micro-fluidic chip is also provided with an elastic film (7), and the elastic film (7) is arranged on the end surfaces of the openings of the main channel (4) and the auxiliary channel (5) of the micro-fluidic chip.

10. The microfluidic chip of claim 9, wherein: and a gap is reserved between the elastic film (7) and the dam body (2).

11. The microfluidic chip of claim 9, wherein: the elastic film (7) has a thickness of 50 to 250 micrometers.

12. The microfluidic chip of claim 11, wherein: the thickness of the elastic film (7) is 75-150 micrometers.

13. The microfluidic chip according to any one of claims 2 to 12, wherein: the micro-fluidic chip further comprises an air passage layer (6), the air passage layer (6) is provided with an air passage connected with an external air pressure source, and the air passage layer (6) and the elastic film (7) are matched to form a pneumatic micro valve.

14. The microfluidic chip of claim 13, wherein: the air passage layer (6) is provided with a concave part in a region corresponding to the crossing part.

15. The microfluidic chip of claim 13, wherein: the pneumatic micro valve position corresponds to the position of the dam body (2).

16. The microfluidic chip of claim 13, wherein: when negative pressure is applied to the air passage in the air passage layer (6), the elastic film (7) under the air passage is bent upwards, and contracts towards the air passage space direction in the air passage layer (6), so that the passage between the dam body (2) and the elastic film (7) is enlarged.

17. The microfluidic chip of claim 2, wherein: the number of the auxiliary channels (5) is two, the number of the grooves (3) is two, the two auxiliary channels (5) are respectively positioned at the left side and the right side of the main channel (4), one groove (3) corresponds to one auxiliary channel (5), and the other groove (3) corresponds to the other auxiliary channel (5).

18. The microfluidic chip of claim 2, wherein: the number of the auxiliary channels (5) is two, the two auxiliary channels (5) are positioned on the same side of the main channel (4), and the grooves (3) correspond to the two auxiliary channels (5).

19. The microfluidic chip of claim 2, wherein: the length (L1) of the groove (3) accounts for 20-80% of the length (L2) of the dam body (2).

20. The microfluidic chip of claim 1, wherein: the interception structure is a single-channel dam body (17), the number of the single-channel dam bodies (17) is two, the single-channel dam bodies are a first single-channel dam body and a second single-channel dam body respectively, the first single-channel dam body is arranged on the auxiliary channel (5), and the second single-channel dam body is arranged on the main channel (4).

21. The microfluidic chip of claim 20, wherein: the single channel dam (17) is substantially strip-shaped.

22. The microfluidic chip of claim 20, wherein: the height of the upper end of the first single-channel dam body does not exceed the height of the auxiliary channel (5), the height of the upper end of the second single-channel dam body does not exceed the height of the main channel (4), the height of the upper end of the first single-channel dam body accounts for more than 50% of the height of the auxiliary channel (5), and the height of the upper end of the second single-channel dam body accounts for more than 50% of the height of the main channel (4).

23. The microfluidic chip of claim 22, wherein: the height of the upper end of the first single-channel dam body accounts for 85-95% of the height of the auxiliary channel (5), and the height of the upper end of the second single-channel dam body accounts for 85-95% of the height of the main channel (4).

24. The microfluidic chip according to any one of claims 20 to 23, wherein: the micro-fluidic chip further comprises an air passage layer (6), the air passage layer (6) is provided with an air passage connected with an external air pressure source, the air passage layer (6) and the elastic film (7) are matched to form a pneumatic micro valve, the pneumatic micro valve comprises a third pneumatic micro valve and a fourth pneumatic micro valve, the third pneumatic micro valve corresponds to the first single-channel dam in position, and the fourth pneumatic micro valve corresponds to the second single-channel dam in position.

25. The microfluidic chip of claim 1, wherein: the intercepting structure is a dam body (2), the dam body (2) is provided with a groove (3), and the groove (3) corresponds to the auxiliary channel (5); the dam body (2) is arranged at the bottom end of the intersection; the height of the upper end of the dam body (2) does not exceed the height of the main channel (4), and the height of the dam body (2) accounts for more than 50% of the height of the main channel (4); the height of the dam body (2) accounts for 85-95% of the height of the main channel (4); the dam body (2) and the liquid path channel of the micro-fluidic chip are of an integrated structure; two supporting legs are arranged on one side of the dam body (2), a groove (3) is formed between the two supporting legs, and the groove (3) corresponds to the position of the auxiliary channel (5) in the microfluidic chip; the width between two supporting legs of the dam body (2) exceeds half of the width of the whole dam body (2); the micro-fluidic chip is also provided with an elastic film (7), and the elastic film (7) is arranged on the end surfaces of the openings of the main channel (4) and the auxiliary channel (5) of the micro-fluidic chip; a gap is reserved between the elastic film (7) and the dam body (2); the elastic film (7) has a thickness of 50 to 250 micrometers; the thickness of the elastic film (7) is 75-150 micrometers; the micro-fluidic chip further comprises an air passage layer (6), the air passage layer (6) is provided with an air passage connected with an external air pressure source, and the air passage layer (6) and the elastic film (7) are matched to form a pneumatic micro valve; the air passage layer (6) is provided with a concave part in a region corresponding to the crossing part; the pneumatic micro valve position corresponds to the position of the dam body (2); when negative pressure is applied to the air passage in the air passage layer (6), the elastic film (7) under the air passage is bent upwards, and contracts towards the air passage space direction in the air passage layer (6), so that the passage between the dam body (2) and the elastic film (7) is enlarged; the number of the auxiliary channels (5) is two, the number of the grooves (3) is two, the two auxiliary channels (5) are respectively positioned at the left side and the right side of the main channel (4), one groove (3) corresponds to one auxiliary channel (5), and the other groove (3) corresponds to the other auxiliary channel (5); or the number of the auxiliary channels (5) is two, the two auxiliary channels (5) are positioned on the same side of the main channel (4), and the grooves (3) correspond to the two auxiliary channels (5); the length (L1) of the groove (3) accounts for 20-80% of the length (L2) of the dam body (2); the interception structure is a single-channel dam body (17), the number of the single-channel dam bodies (17) is two, the first single-channel dam body is arranged on the auxiliary channel (5), and the second single-channel dam body is arranged on the main channel (4); the single-channel dam body (17) is of a substantially long strip shape; the height of the upper end of the first single-channel dam body does not exceed the height of the auxiliary channel (5), the height of the upper end of the second single-channel dam body does not exceed the height of the main channel (4), the height of the upper end of the first single-channel dam body accounts for more than 50% of the height of the auxiliary channel (5), and the height of the upper end of the second single-channel dam body accounts for more than 50% of the height of the main channel (4); the height of the upper end of the first single-channel dam body accounts for 85-95% of the height of the auxiliary channel (5), and the height of the upper end of the second single-channel dam body accounts for 85-95% of the height of the main channel (4); the micro-fluidic chip further comprises an air passage layer (6), the air passage layer (6) is provided with an air passage connected with an external air pressure source, the air passage layer (6) and the elastic film (7) are matched to form a pneumatic micro valve, the pneumatic micro valve comprises a third pneumatic micro valve and a fourth pneumatic micro valve, the third pneumatic micro valve corresponds to the first single-channel dam in position, and the fourth pneumatic micro valve corresponds to the second single-channel dam in position.