CN219555891U - Intermittent immersion type bioreactor - Google Patents

Abstract

The utility model discloses an intermittent immersion bioreactor, which belongs to the technical field of plant preparation and culture, and comprises the following components: the culture device comprises a culture bottle, a nutrition bottle, a culture tray, a nutrition liquid channel and a compressed air inlet and outlet pipe; the nutrient flask is connected with the downside of the culture flask in a rotating way, the culture tray is horizontally placed on the downside inside the culture flask, the bottom of the culture flask is connected with a nutrient solution channel, the nutrient solution channel stretches into the inside of the nutrient flask, one side of the nutrient flask is externally connected with a compressed air inlet, and nutrient solution is stored in the nutrient flask. The utility model has the advantages of recycling culture solution, easy regulation and control of various culture conditions, good sealing effect of the reactor, difficult pollution, continuous culture, high survival rate of cultured plants, fast growth, less subculture times, remarkably enhanced stress resistance of regenerated plants and good yield and quality.

Description

Intermittent immersion type bioreactor

Technical Field

The utility model belongs to the technical field of plant preparation and culture, and particularly relates to an intermittent leaching bioreactor.

Background

The bioreactor is used as a device for rapidly culturing plants in various fields, and can be used for growth, proliferation and differentiation. Plants grown in such bioreactors are usually planted in a growth medium, the growth medium provides nutrition for the plants, and the nutrient solution is used for soaking the root system, but the long-time soaking of the root system can lead to incapacity of cells to breathe, rot of the root system, death of the final plant, and unfavorable for rapid plant cultivation, so that the soaking state of the plant root system needs to be regulated regularly, and meanwhile, the carbon dioxide concentration in the device and how to timely discharge oxygen generated by the plants can also influence the growth of the plants.

In the plant culture device of the prior art Chinese patent (CN 202020899374.2), the water inlet is matched with the water outlet, the root system soaking state is adjusted, the nutrient solution cannot be recycled, the sealing effect of the whole device is poor, the survival rate of plants is low, and the content of a reactor is easy to be polluted.

Therefore, how to provide a high-efficiency batch-type bioreactor is a problem to be solved by those skilled in the art.

Disclosure of Invention

In view of the above, the utility model provides an intermittent immersion bioreactor, which has the advantages of recycling culture solution, easy regulation and control of various culture conditions, good sealing effect, difficult pollution, high plant survival rate, continuous culture, reduced subculture times, remarkably enhanced stress resistance of regenerated plants, and high growth and quality of regenerated plants.

In order to achieve the above purpose, the present utility model adopts the following technical scheme:

a batch immersion bioreactor comprising: the culture device comprises a culture bottle, a nutrition bottle, a culture tray, a nutrition liquid channel and a compressed air inlet; the nutrient flask is connected with the downside of the culture flask in a rotating way, the culture tray is horizontally placed on the downside inside the culture flask, the bottom of the culture flask is connected with a nutrient solution channel, the nutrient solution channel stretches into the inside of the nutrient flask, one side of the nutrient flask is externally connected with a compressed air inlet, and nutrient solution is stored in the nutrient flask.

Further, the culture tray is provided with through holes for nutrient solution at equal intervals.

Further, the top of the culture bottle is screwed with a rotary upper cover.

Further, the rotary upper cover is provided with an exhaust pipe in a penetrating mode, and a filter screen for preventing microbial contamination is arranged in the exhaust pipe.

Further, a compressed air inlet filter screen valve is arranged on one side branch of the compressed air inlet and outlet pipe, and a compressed air emptying filter screen valve is arranged on the other side branch of the compressed air inlet and outlet pipe.

The utility model has the beneficial effects that:

the utility model has simple structure and convenient use, the root system of the plant is planted on the nutrient solution through hole of the culture tray, the culture bottle and the nutrient bottle are screwed, the rotary upper cover is screwed, the nutrient bottle forms a closed space, air is input into the nutrient bottle through the compressed air inlet and outlet pipe, pressure is generated, the nutrient solution flows upwards from the nutrient solution channel to the lower part of the culture tray in the culture bottle, nutrition is provided for the plant, the exhaust pipe provided with the filter screen preventing microbial contamination is used for continuously exhausting, the higher fresh air content in the culture bottle is ensured, after the plant is soaked for a period of time, the compressed air inlet filter screen valve is closed, the compressed air emptying filter screen valve is opened, the nutrient solution is refluxed to the nutrient bottle, the intermittent soaking is completed, and then the steps are repeated, so that the utility model has the advantages of recycling the nutrient solution, easy regulation of various culture conditions, good sealing effect of the reactor, difficult pollution, high survival rate of the plant, continuous culture, reduced times of the regeneration plant stress resistance, obvious enhancement, and high growth and high quality of the regenerated plant.

Drawings

In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present utility model, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.

Fig. 1 is a schematic view of the structure of the present utility model.

Fig. 2 is a top view of the structure of the present utility model.

Wherein, in the figure:

1-a culture bottle; 2-a nutrition bottle; 3-a culture tray; 4-nutrient solution channels; 5-culture plate mesh; 6-rotating the upper cover; 7-exhaust pipe; 8-compressed air inlet and outlet pipes; 9 compressed air inlet filter screen valve; 10-compressed air emptying filter screen valve.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Referring to fig. 1-2, the present utility model provides a batch immersion bioreactor comprising: a culture bottle 1, a nutrition bottle 2, a culture tray 3, a nutrition liquid channel 4 and a compressed air inlet and outlet pipe 8; the nutrient bottle 2 is connected to the lower side of the culture bottle 1 in a screwed mode, the culture tray 3 is horizontally placed on the lower side inside the culture bottle 1, the bottom of the culture bottle 1 is connected with a nutrient solution channel 4, the nutrient solution channel 4 stretches into the nutrient bottle 2, one side of the nutrient bottle 2 is externally connected with a compressed air inlet pipe 8, nutrient solution is stored in the nutrient bottle 2, the compressed air device is preferably a nitrogen dioxide pressure pump, and a plurality of intermittent immersion bioreactors can be connected through a manifold by using one power pump. When the nutrition bottle 2 is screwed with the culture bottle 1, a rubber gasket can be arranged to ensure the sealing effect during screwing.

The culture tray 3 is provided with the nutrient solution through holes 5 at equal intervals in a penetrating way, and the adjacent through holes in different rows can be arranged in a staggered way, so that the space of the culture bottle 1 is fully utilized. The rotary upper cover 6 is screwed on the top seal of the culture bottle 1, and a rubber gasket can be arranged to ensure the sealing effect. An exhaust pipe 7 is arranged on the rotary upper cover 6 in a penetrating mode, and a filter screen for preventing microbial contamination is arranged in the exhaust pipe 7. A compressed air inlet filter screen valve 9 is arranged on one side branch of the compressed air inlet and outlet pipe 8, and a compressed air emptying filter screen valve 10 is arranged on the other side branch. The device can be externally connected with an automatic timer, and the compressed air inlet filter screen valve 9 and the compressed air discharging filter screen valve 10 are opened or closed at regular time. The device can set automatic cycle intermittent time according to the condition of plants, maintain stable culture environment and can culture multiple generations of plants in a short time. The filter screens in the compressed air inlet filter screen valve 9 and the compressed air discharge filter screen valve 10 may also use a filter screen for preventing microbial contamination.

The utility model has simple structure and convenient use, when in use, plants are planted on the nutrient solution through holes 5 of the culture tray 3, then the culture tray 3 is horizontally placed in the culture bottle 1, the lower side of the culture bottle 1 is tightly screwed with the nutrient bottle 2, the upper side of the culture bottle 1 is tightly screwed with the rotary upper cover 6, then the compressed air inlet pipe 8 is communicated, so that the compressed air enters the nutrient bottle 2, the nutrient solution is pressed into the lower part of the culture tray 3 in the culture bottle 1 through the nutrient solution channel 4, nutrition is provided for plants at regular time, meanwhile, redundant air and generated oxygen are discharged through the exhaust pipe 7 with the filter screen, after the soaking time is reached, the pressure in the nutrient bottle 2 is reduced, the nutrient solution flows back to the nutrient bottle 2, the long-time plant root system soaking of the nutrient solution is avoided, the redundant air can be discharged through the exhaust pipe 7 with the filter screen, the compressed air inlet pipe 8 which is also connected to the next reactor in an inflatable manner, all the filter screen can be opened and closed through the compressed air inlet valve 9 and the compressed air discharge valve 10 which are controlled by an external automatic timer, and all the filter screens can be repeatedly used for sterilizing the filter screen assemblies after the filter screen is opened and the intermittent pressure is reduced. Therefore, the utility model has the advantages of recycling culture solution, easy regulation and control of various culture conditions, good sealing effect of the reactor, difficult pollution, high plant survival rate, continuous culture, reduced subculture times, obviously enhanced stress resistance of regenerated plants, and high growth and quality of the regenerated plants.

The embodiments are described in a progressive manner in this specification, and for the apparatus disclosed in the embodiments, since it corresponds to the method disclosed in the embodiments, the description is relatively simple, and the relevant points refer to the description of the method section.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present utility model. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the utility model. Thus, the present utility model is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (5)

1. A batch-dip bioreactor, comprising: a culture bottle (1), a nutrition bottle (2), a culture tray (3), a nutrition liquid channel (4) and a compressed air inlet and outlet pipe (8); the nutrient solution bottle (2) is screwed on the lower side of the culture bottle (1), the culture tray (3) is horizontally placed on the lower side of the inner part of the culture bottle (1), the bottom of the culture bottle (1) is connected with a nutrient solution channel (4), the nutrient solution channel (4) stretches into the nutrient bottle (2), and one side of the nutrient bottle (2) is externally connected with a compressed air inlet and outlet pipe (8); the nutrient solution is stored in the nutrient bottle (2).

2. Batch-wise bioreactor according to claim 1, characterized in that the culture trays (3) are provided with through holes (5) for the nutrient solution at equal intervals.

3. Batch-wise bioreactor according to claim 1, characterized in that the top of the flask (1) is screwed with a rotating upper cover (6).

4. The intermittent immersion bioreactor as claimed in claim 3, characterized in that the rotary upper cover (6) is provided with an exhaust pipe (7) in a penetrating way, and a filter screen for preventing microbial contamination is arranged in the exhaust pipe (7).

5. Intermittent immersion bioreactor according to claim 1, characterized in that the compressed air inlet and outlet pipe (8) is provided with a compressed air inlet filter screen valve (9) on one side branch and a compressed air vent filter screen valve (10) on the other side branch.