CN219546969U

CN219546969U - Microfluidic detection device

Info

Publication number: CN219546969U
Application number: CN202223524848.3U
Authority: CN
Inventors: 蒋兴宇; 王斗; 郑锦涛
Original assignee: Southwest University of Science and Technology
Current assignee: Southwest University of Science and Technology
Priority date: 2022-12-22
Filing date: 2022-12-22
Publication date: 2023-08-18
Anticipated expiration: 2032-12-22

Abstract

The utility model provides a microfluidic detection device, and relates to the field of medical detection; the microfluidic detection device includes: the device comprises a shell and a detection assembly, wherein the detection assembly is positioned in the shell and comprises a driving piece, an imaging assembly, a fixing assembly and a control module, and the fixing assembly comprises an objective table and a plurality of jacking pieces; one ends of the plurality of jacking pieces penetrate out and are movably arranged on the objective table; the driving end of the driving piece is penetrated with a plurality of height adjusting pieces which are in one-to-one correspondence with the plurality of jacking pieces at intervals, and the driving piece is used for driving the plurality of height adjusting pieces to rotate simultaneously so as to adjust the abutting positions of the height adjusting pieces and the other ends of the corresponding jacking pieces at the same time to adjust the jacking height of the corresponding jacking pieces; the control module is in communication connection with the imaging assembly and the driving member. The microfluidic detection device provided by the embodiment of the utility model can improve the convenience of use by reducing the volume.

Description

Microfluidic detection device

Technical Field

The embodiment of the utility model relates to the field of medical detection, but is not limited to the field of medical detection, in particular to a microfluidic detection device.

Background

In the field of molecular detection, especially in the field of nucleic acid detection, the requirements for operators and experimental environments are very high, and usually, collected samples need to be sent to a biological laboratory for pretreatment of the samples by professionally trained personnel, a reaction system is configured, and then the samples are put into a special reaction instrument for amplification and signal reading. However, because of limitations of professionals and experimental environments, the versatility and convenience of detection are poor, and thus new methods are needed to solve these problems. In the related art, the collected sample is usually added into a matched microfluidic chip based on a microfluidic technology, and then the microfluidic chip is put into an instrument to automatically perform all reaction processes, so that the whole process does not need to be manually participated, and the requirements on the environment are not very high due to the closed reaction process, so that the method is widely used. However, in the related art, the devices for microfluidic detection often have more control points for performing reaction by using the microfluidic chip and devices such as an imaging component and the like are required to be deployed for visual tracking, so that the whole volume of the devices for microfluidic detection is larger, and the devices are not convenient to use; therefore, the microfluidic detection device with higher integration and smaller volume is continued to improve the convenience of use.

Disclosure of Invention

The following is a summary of the subject matter described in detail herein. This summary is not intended to limit the scope of the claims.

The embodiment of the utility model provides a microfluidic detection device, which can improve the convenience of use.

According to an embodiment of the present utility model, a microfluidic detection device includes:

a housing;

the detection assembly is positioned in the shell and comprises a driving piece, an imaging assembly, a fixing assembly and a control module, wherein the fixing assembly comprises an objective table and a plurality of jacking pieces; one ends of the plurality of jacking pieces penetrate out and are movably arranged on the objective table; the driving end of the driving piece is penetrated with a plurality of height adjusting pieces in one-to-one correspondence with the plurality of jacking pieces at intervals, and the driving piece is used for driving the plurality of height adjusting pieces to rotate simultaneously to control the abutting state of each height adjusting piece and the other end of the corresponding jacking piece; the control module is in communication connection with the imaging assembly and the driving member.

Therefore, the above embodiment of the present utility model has at least the following advantages: through wearing to establish a plurality of height-adjusting parts on a driving piece with the interval to adjust through the butt position of each height-adjusting part with the other end of the jacking piece that corresponds and can realize simultaneously controlling each control valve of the micro-fluidic chip that is located on the objective table respectively through a driving piece, and to the driving piece, it only need provide pivoted driving force can, consequently the constitution of each part in the detection subassembly can be compacter, thereby make the whole volume of casing reduce. Therefore, the microfluidic detection device in the embodiment of the utility model can improve the convenience of use by reducing the volume.

According to some embodiments of the utility model, the object stage includes a first object carrying plate and a second object carrying plate that are disposed at intervals, two ends of the lifting member respectively penetrate through the first object carrying plate and the second object carrying plate, and the lifting member is movably disposed along a lifting direction relative to the first object carrying plate and the second object carrying plate.

According to some embodiments of the utility model, each of the lifting members is sleeved with an elastic member, and the elastic member is located between the first carrying plate and the second carrying plate.

According to some embodiments of the utility model, the detection assembly is further provided with a plurality of heating modules, and the heating modules are embedded in the first carrier plate; the heating module is in communication connection with the control module.

According to some embodiments of the utility model, the detection assembly further comprises a pressure supply module comprising a transmission mechanism for causing the syringe to output positive or negative pressure, and a syringe, the transmission mechanism being electrically connected to the control module.

According to some embodiments of the utility model, the detection assembly further comprises a washing module comprising a peristaltic pump for pumping liquid out of the washing bottle and a washing bottle, the peristaltic pump being electrically connected to the control module.

According to some embodiments of the utility model, the driving member, the cleaning module and the pressure supply module are all fixed to the first side wall of the housing and are sequentially distributed at intervals along the first side wall; a through hole is provided in the second side wall of the housing adjacent to the pressure supply module.

According to some embodiments of the utility model, the imaging assembly is fixed to a third side wall opposite the second side wall, the third side wall having a stiffener disposed thereon.

According to some embodiments of the utility model, the housing is arranged to be flipped open adjacent to the fourth side wall of the driving member.

According to some embodiments of the utility model, a side wall adjacent to the fourth side wall is provided with a display screen, the display screen being electrically connected to the control module.

Additional features and advantages of the utility model will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the utility model. The objectives and other advantages of the utility model will be realized and attained by the structure particularly pointed out in the written description and claims hereof as well as the appended drawings.

Drawings

The accompanying drawings are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate and do not limit the utility model.

FIG. 1 is a schematic cross-sectional view of a microfluidic detection device according to an embodiment of the present utility model;

FIG. 2 is an enlarged schematic view of a portion of a microfluidic detection device according to an embodiment of the present utility model;

FIG. 3 is a schematic cross-sectional view of a microfluidic detection device according to an embodiment of the present utility model at another viewing angle

FIG. 4 is a schematic diagram of a microfluidic chip for detection by a microfluidic detection device according to an embodiment of the present utility model;

fig. 5 is a schematic structural diagram of a microfluidic detection device according to an embodiment of the present utility model.

Reference numerals:

the casing 100, the first side wall 110, the second side wall 120, the through hole 121, the third side wall 130, the reinforcing rib 131, the fourth side wall 140,

Detection assembly 200, drive member 210, imaging assembly 220, fixture assembly 230, stage 231, first carrier plate 2311, second carrier plate 2312, lift member 232, control module 240, height adjustment member 250, elastic member 260, heating module 270, pressure supply module 280, transmission mechanism 281, syringe 282, cleaning module 290, peristaltic pump 291, cleaning bottle 292, and,

Microfluidic chip 310, display 320, pillars 330.

Detailed Description

The present utility model will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present utility model more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the utility model.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this utility model belongs. The terminology used herein is for the purpose of describing embodiments of the utility model only and is not intended to be limiting of the utility model. The terms "first," "second," "third," "fourth," and the like in the description of the utility model and in the above figures, if any, are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order.

Furthermore, the described features, structures, or characteristics may be combined in any suitable manner in one or more embodiments. In the following description, numerous specific details are provided to give a thorough understanding of embodiments of the disclosure. One skilled in the relevant art will recognize, however, that the disclosed aspects may be practiced without one or more of the specific details, or with other methods, components, devices, steps, etc. In other instances, well-known methods, devices, implementations, or operations are not shown or described in detail to avoid obscuring aspects of the disclosure.

Referring to fig. 1 to 5, a microfluidic detection device according to an embodiment of the present utility model includes:

a housing 100;

the detection assembly 200, the detection assembly 200 is located in the housing 100, the detection assembly 200 includes a driving member 210, an imaging assembly 220, a fixing assembly 230 and a control module 240, the fixing assembly 230 includes a stage 231 and a plurality of lifting members 232; one end of the plurality of lifting members 232 penetrates out and is movably arranged on the object stage 231; the driving end of the driving member 210 is penetrated with a plurality of height adjusting members 250 corresponding to the plurality of jacking members 232 one by one at intervals, and the driving member 210 is used for driving the plurality of height adjusting members 250 to rotate simultaneously to control the abutting state of each height adjusting member 250 and the other end of the corresponding jacking member 232; the control module 240 is communicatively coupled to the imaging assembly 220 and the driver 210.

Therefore, the above embodiment of the present utility model has at least the following advantages: by arranging the plurality of height adjusting members 250 on one driving member 210 at intervals, and adjusting the abutting positions of the height adjusting members 250 and the other ends of the corresponding jacking members 232, the control of the control valves of the microfluidic chip 310 on the stage 231 by one driving member 210 can be realized at the same time, and only a rotational driving force is required to be provided for the driving member 210, so that the components of the detection assembly 200 can be more compact, and the overall volume of the housing 100 can be reduced. Therefore, the microfluidic detection device in the embodiment of the utility model can improve the convenience of use by reducing the volume.

It should be noted that, the microfluidic detection device itself may provide all environments for detection, so after the volume of the microfluidic detection device is reduced, the convenience of carrying and placing of the microfluidic detection device may be further improved, so as to adapt to more scenes, and further improve the convenience of use.

It should be noted that the driving member 210 may be directly configured as a stepping motor. The plurality of height adjusters 250 may be provided according to the number of actual microfluidic chips 310. In some embodiments, a plurality of height adjustment members 250 are detachably connected with the driving end of the driving member 210, so that different microfluidic chips 310 may be further adapted.

It should be noted that, as the driving member 210 rotates, the profile of the height adjusting member 250 opposite to the lifting member 232 changes, when the height adjusting member 250 abuts against the lifting member 232, an upward pushing force may be provided, and after the abutment, as the driving member 210 rotates, when the outer profile of the height adjusting member 250 further protrudes outwards, continuous lifting of the lifting member 232 may be achieved, so that the valve corresponding to the lifting member 232 may be lifted. Illustratively, as shown in FIG. 4, reference numerals (1) (2) (3) with red color in the drawing are valves, and reference numerals (1) (2) (3) (4) (5) with black color are respectively a sample cavity, a reagent cavity, a reaction cavity, a hybridization cavity and a waste liquid cavity; the height adjusting members 250 are correspondingly provided with 3 height adjusting members, when the 3 valves are required to be closed simultaneously, the outer profiles of the 3 height adjusting members 250 are consistent and are all arranged in a protruding mode in a certain area, and when the driving member 210 rotates the height adjusting members 250 to the edge of the protruding area, the height adjusting members 250 are abutted with the jacking members 232 until the jacking members 232 close the valves. When all the 3 valves need to be closed respectively, the positions of the raised areas of the 3 height adjusting members 250 are different, and the rotation angle of one driving member 210 is matched for each raised area position, so that only the height adjusting member 250 corresponding to the angle can lift the corresponding lifting member 232 by controlling the rotation angle of the driving member 210, and therefore, one driving member 210 can control a plurality of valves to be closed respectively.

The imaging assembly 220 includes an objective lens, a beam splitter, a light source with a collimating lens, an excitation filter, an absorption filter, a CCD camera, and the like. Wherein the objective lens is aligned with the imaging region. The imaging assembly 220 may be disposed above the stage 231 according to the actual space, or may be disposed on the stage 231 side, so long as the field of view of the objective lens is aligned with the imaging region. Referring to fig. 3, imaging assembly 220 is secured to post 330 by a connection plate (connection plate not shown) to provide support to imaging assembly 220 by post 330. Specifically, those skilled in the art can arrange the driving arrangement in the housing 100 and the remaining spaces of the vertical space and the longitudinal space.

The control module 240 controls the reaction of the whole microfluidic chip 310, and can send a preset program to a lower computer for execution through upper computer software, and can manually issue instructions to change conditions to adapt to different reactions.

It should be noted that the lifting member 232 may be configured as a lifting column, where the lifting member 232 corresponds to each control valve on the microfluidic chip 310. The height adjuster 250 may be provided as a sheet cam.

As can be appreciated, referring to fig. 2, the stage 231 includes a first carrier plate 2311 and a second carrier plate 2312 that are disposed at intervals, two ends of the lifting member 232 respectively penetrate through the first carrier plate 2311 and the second carrier plate 2312, and the lifting member 232 is movably disposed relative to the first carrier plate 2311 and the second carrier plate 2312 along a lifting direction; the lifting member 232 is sleeved with an elastic member 260, and the elastic member 260 is located between the first carrier plate 2311 and the second carrier plate 2312.

It should be noted that, by setting the first carrier plate 2311 and the second carrier plate 2312, the movement direction of the lifting member 232 is fixed, so that shake is avoided, and the valve is controlled more accurately.

It can be appreciated that each lifting member 232 is sleeved with an elastic member 260, and the elastic member 260 is located between the first carrier plate 2311 and the second carrier plate 2312.

It should be noted that, by providing the elastic member 260 so that the lifting member 232 descends, the time for opening the valve is further shortened.

It can be appreciated that referring to fig. 2, the detecting assembly 200 is further provided with a plurality of heating modules 270, and the heating modules 270 are embedded in the first carrier plate 2311; the heating module 270 is communicatively coupled to the control module 240.

It should be noted that at least one heating module 270 is provided, and each heating module 270 is configured to set a reaction chamber of the microfluidic chip 310, so that the temperature can be accurately controlled independently. The heating module 270 may be detachably disposed on the first carrier plate 2311 to achieve matching of different microfluidic chips 310. When the microfluidic chip 310 is placed on the first carrier plate 2311, the microfluidic chip 310 is limited at a fixed position by a limiting device, and the area to be heated of the microfluidic chip 310 corresponds to the heating module 270 one by one, and at this time, the temperature adjustment and the heating time control can be performed by the control module 240 according to the reaction process.

It should be noted that, since the heating module 270 is embedded in the first carrier plate 2311, no additional space is required.

It will be appreciated that referring to fig. 3, the detection assembly 200 further includes a pressure supply module 280, the pressure supply module 280 including a transmission mechanism 281 and a syringe 282, the transmission mechanism 281 being configured to cause the syringe 282 to output either positive or negative pressure, the transmission mechanism 281 being electrically connected to the control module 240.

It should be noted that the transmission mechanism 281 may include a stepper motor and a screw assembly to enable pushing and pulling of the syringe 282 to provide positive or negative pressure.

It should be noted that, the syringe 282 is connected to the microfluidic chip 310 through a hose to provide positive or negative pressure to the microfluidic chip 310. By means of hoses, the positional constraints on the pressure supply module 280 may be reduced.

It will be appreciated that the detection assembly 200 further comprises a washing module 290, the washing module 290 comprising a peristaltic pump 291 and a washing bottle 292, the peristaltic pump 291 being adapted to pump out liquid from the washing bottle 292, the peristaltic pump 291 being electrically connected to the control module 240.

It should be noted that, after the reaction of the microfluidic chip 310 is completed, the control module 240 controls the peristaltic pump 291 to quantitatively pump the cleaning solution in the cleaning bottle 292 into the microfluidic chip 310 to clean and blow-dry the gold chips in the hybridization cavity; it should be noted that, in some embodiments, the output of the purge bottle 292 and the output of the syringe 282 are independent, and in other embodiments, the purge bottle 292 and the output of the syringe 282 share a microfluidic input port, where a three-way valve may be used to connect the output and input pipes of the purge module 290, the pressure supply module 280, and the microfluidic chip 310, and the independent operation between the pipes is controlled by a pinch valve.

It can be appreciated that the driving member 210, the cleaning module 290 and the pressure supply module 280 are all fixed to the first side wall 110 of the housing 100 and are sequentially spaced apart along the first side wall 110; the second side wall 120 of the housing 100 adjacent to the pressure supply module 280 is provided with a through hole 121.

The first sidewall 110 is a bottom of the housing 100, and the through hole 121 is disposed near the pressure supply module 280, and the wind entering from the through hole 121 is far away from the stage 231, so that the effect of heat dissipation in the housing 100 and the effect of the micro fluidic chip 310 can be reduced.

It is understood that the imaging assembly 220 is fixed to the third sidewall 130 opposite to the second sidewall 120, and the third sidewall 130 is provided with a reinforcing rib 131.

It should be noted that, by adding the reinforcing ribs 131, the stability of the housing 100 is improved.

It will be appreciated that, referring to fig. 3 and 5, the housing 100 is disposed adjacent the fourth side wall 140 of the driving member 210 as a flip door.

It is understood that the side wall adjacent to the fourth side wall 140 is provided with a display screen 320, and the display screen 320 is electrically connected with the control module 240.

It should be noted that, by providing the display screen 320 on the housing 100, the state of the microfluidic chip 310 can be directly observed, and the terminal device is not required to be additionally provided for control, so that the operation is more convenient.

It should be noted that, in the microfluidic detection device according to the above embodiment of the present utility model, the first side wall 110 at the bottom is sequentially disposed, the through hole 121 is formed at the bottom of the second side wall 120 adjacent to the first side wall 110, so that the air in the housing 100 can circulate, and the imaging assembly 220 is disposed on the third side wall 130 opposite to the second side wall 120, so that the imaging assembly 220 is located above the stage 231.

While the preferred embodiment of the present utility model has been described in detail, the present utility model is not limited to the above embodiment, and various equivalent modifications and substitutions can be made by those skilled in the art without departing from the spirit of the present utility model, and these equivalent modifications and substitutions are intended to be included in the scope of the present utility model as defined in the appended claims.

Claims

1. A microfluidic detection device, comprising:

a housing;

2. The microfluidic detection device according to claim 1, wherein the stage comprises a first carrier plate and a second carrier plate which are arranged at intervals, two ends of the lifting member respectively penetrate through the first carrier plate and the second carrier plate, and the lifting member is movably arranged relative to the first carrier plate and the second carrier plate along the lifting direction.

3. The microfluidic detection device according to claim 2, wherein each of the lifting members is sleeved with an elastic member, and the elastic member is located between the first carrier plate and the second carrier plate.

4. The microfluidic detection device according to claim 2, wherein the detection assembly is further provided with a plurality of heating modules, and the heating modules are embedded in the first carrier plate; the heating module is in communication connection with the control module.

5. The microfluidic detection device of claim 1, wherein the detection assembly further comprises a pressure supply module comprising a transmission mechanism and a syringe, the transmission mechanism for causing the syringe to output positive or negative pressure, the transmission mechanism being electrically connected to the control module.

6. The microfluidic detection device of claim 5, wherein the detection assembly further comprises a washing module comprising a peristaltic pump and a washing bottle, the peristaltic pump for pumping liquid from the washing bottle, the peristaltic pump being electrically connected to the control module.

7. The microfluidic detection device of claim 6, wherein the drive member, the cleaning module, and the pressure supply module are all fixed to the first sidewall of the housing and are sequentially spaced apart along the first sidewall; a through hole is provided in the second side wall of the housing adjacent to the pressure supply module.

8. The microfluidic detection device according to claim 7, wherein the imaging assembly is fixed to a third side wall opposite the second side wall, the third side wall having a stiffener disposed thereon.

9. The microfluidic detection device of claim 1, wherein the housing is disposed adjacent to the fourth side wall of the driver as a flip-open door.

10. The microfluidic detection device according to claim 9, wherein a side wall adjacent to the fourth side wall is provided with a display screen, the display screen being electrically connected to the control module.