CN219546950U - Support for placing cell culture bottle and controlling water of cell culture bottle - Google Patents
Support for placing cell culture bottle and controlling water of cell culture bottle Download PDFInfo
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- CN219546950U CN219546950U CN202320877976.1U CN202320877976U CN219546950U CN 219546950 U CN219546950 U CN 219546950U CN 202320877976 U CN202320877976 U CN 202320877976U CN 219546950 U CN219546950 U CN 219546950U
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- cell culture
- water
- elastic piece
- water receiving
- horizontal rods
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The utility model discloses a bracket for placing a cell culture bottle and controlling water, which comprises the following components: the enclosure frame is formed by fixedly connecting two first horizontal rods and two second horizontal rods end to end in sequence, a first elastic piece is arranged between the two first horizontal rods, one end of the first elastic piece is fixedly connected with one first horizontal rod, the other end of the first elastic piece is fixedly connected with the other first horizontal rod, one end of the second elastic piece is fixedly connected with one second horizontal rod, the other end of the second elastic piece is fixedly connected with the other second horizontal rod, and the second elastic piece is used for forming an elastic accommodating cavity in an interweaving fit with the first elastic piece, and the accommodating cavity is used for accommodating a cell culture bottle; the utility model improves the containing quantity of the cell culture bottles in unit area, has good water control effect, is not easy to cause pollution, is beneficial to the quantity requirement of the cell culture bottles for the subsequent biological vaccine production, and further improves the biological vaccine yield.
Description
Technical Field
The utility model belongs to the field of biological vaccines, and particularly relates to a bracket for placing a cell culture bottle and controlling water.
Background
The vaccine is a biological product which is prepared by taking natural or artificial modified microorganisms, parasites and components (proteins or nucleic acids) or products (toxins) thereof as materials and adopting corresponding technologies such as biology, molecular biology or biochemistry, bioengineering and the like and is used for preventing epidemic diseases, and the vaccination is the most economical and effective measure for preventing and controlling infectious diseases. The most critical aspect of vaccine production is to obtain the corresponding antigen, and the method for culturing the antigen mainly comprises tissue culture, chemical synthesis and in vitro cell culture. The in vitro cell culture antigen is an important means of the forefront of the prior art, the most economic benefit, the fastest production and the most reliable effect, and a culture carrier or a cell culture bottle is used for culturing the antigen used for the vaccine through primary cells or passage cell lines.
The cell culture bottle is generally applied to cell culture work together with a bottle rotating machine, so that cell culture can be changed from static state to dynamic state, and the cell culture bottle is a simplified bioreactor and is suitable for culturing suspension cells and adherent cells. Cell transfer bottle culture is a cell transition stage before the maturation of large-scale culture technology, is an important step for optimizing various experimental conditions from laboratory scale-up to workshop production, and can be used as a way for preparing a bioreactor inoculated cell culture medium.
Cell-flask culture techniques have the advantage of providing a large growth surface for cells; slight rotation prevents the possible effects of certain components formed in the culture broth on cell growth; the cells are only covered with a thin layer of culture solution for most of the time, which is beneficial to gas exchange; the structure is simple, the investment is low, the technology is mature, and the repeatability is good; the amplification can be achieved by simply increasing the number of the cell transfer bottles. Thus, culturing antigens using cell culture flasks is often widely used in practical production, especially during the vaccine laboratory research phase.
The cell culture bottle is used as a container for cell culture, the cleanliness of the cell culture bottle influences the cell wall-attached growth, and the glass cell culture bottle which is repeatedly used needs to be thoroughly controlled by water under the condition of cleaning, otherwise, the residual water stain can influence the cell wall-attached growth, and even cause cell pollution. At present, the cell culture bottle is used for controlling water by inverting the cleaned cell culture bottle on a wooden frame, and the cell culture bottle is generally designed in a single-layer manner, because the multi-layer design can cause the water control of the upper cell culture bottle to drop on the lower cell culture bottle, the quantity of the water control cell culture bottles is limited or the water control bottle frame is increased to meet the requirement, and the occupied area is increased; no isolation protection measures are provided between the cell culture flasks for controlling water, and the phenomenon that the cell culture flasks collide with each other and break often occurs; the controlled water flow is on the ground, which is not beneficial to the clean and sanitary of the place; wooden materials are easy to mildew or bacteria to grow when wetted, and small wood dust particles can be fallen off to cause secondary pollution of cells.
Disclosure of Invention
The utility model aims to provide a bracket for placing and controlling water in a cell culture bottle, which solves the problems of safe placement and water control of the cell culture bottle used for vaccine manufacture.
The utility model adopts the following technical scheme: a rack for holding and controlling water in a cell culture flask, comprising:
the enclosure frame is horizontally arranged and is formed by fixedly connecting two first horizontal rods and two second horizontal rods end to end in sequence, the two first horizontal rods are oppositely arranged, the two second horizontal rods are oppositely arranged and are vertical to the trend of the first horizontal rods,
the first elastic piece is arranged between the two first horizontal rods and is vertical to the trend of the first horizontal rods, one end of the first elastic piece is fixedly connected with one first horizontal rod, the other end of the first elastic piece is fixedly connected with the other first horizontal rod,
the second elastic piece is arranged between the two second horizontal rods and is vertical to the trend of the second horizontal rods, one end of the second elastic piece is fixedly connected with one second horizontal rod, the other end of the second elastic piece is fixedly connected with the other second horizontal rod and is used for forming an elastic accommodating cavity in an interweaving fit with the first elastic piece, the accommodating cavity is used for accommodating a cell culture bottle,
limiting plate, the level sets up, is located the below that encloses the fender frame, has seted up spacing hole on it, and spacing hole is used for when need controlling water to the cell culture bottle for the opening of cell culture bottle is downthehole and its neck stretches into spacing and then controls water to the cell culture bottle, and the limiting plate is used for when needs accomodate the cell culture bottle, supports the bottom of cell culture bottle.
Further, a plurality of spacing holes have been seted up on the limiting plate, and first elastic component and second elastic component also are provided with many, and then enclose into a plurality of holding cavities, and first elastic component and second elastic component still are used for when cell culture bottle opening is upwards or down, prevent that two blake bottles in the adjacent holding cavity from taking place to collide, and then avoid the blake bottle to damage.
Further, the periphery of each accommodating cavity is surrounded by the first elastic piece and the second elastic piece, the vertical axis of each accommodating cavity coincides with the axis of the corresponding limiting hole, and the first elastic piece or the second elastic piece between two adjacent accommodating cavities is not shared.
Further, the method further comprises the following steps: a water receiving mechanism is arranged on the water tank,
the water receiving mechanism comprises:
the water receiving frame is horizontally arranged and is formed by fixedly connecting four third horizontal rods end to end in sequence,
the water receiving cross rod is horizontally arranged, two ends of the water receiving cross rod are respectively fixed on two third horizontal rods which are oppositely arranged,
the water receiving longitudinal rod is horizontally arranged, two ends of the water receiving longitudinal rod are respectively fixed on the other two third horizontal rods which are oppositely arranged,
the water receiving disc is fixed at the intersection of the water receiving cross rod and the water receiving longitudinal rod, the axis of the water receiving disc is coincident with the axis of the limiting hole and is used for receiving water flowing out of the cell culture bottle during water control.
Further, each water receiving disc is communicated with the underground water through a diversion pipe.
Further, the method further comprises the following steps: four support pole setting, vertical setting, its upper end and enclose fender frame fixed connection, its middle part respectively with limiting plate and water receiving frame fixed connection, its lower extreme is used for supporting at ground, then supports whole accuse water support.
The beneficial effects of the utility model are as follows:
1. the utility model improves the containing quantity of the cell culture bottles in unit area, has good water control effect, is not easy to cause pollution, is beneficial to the quantity requirement of the cell culture bottles for the subsequent biological vaccine production, and further improves the biological vaccine yield;
2. according to the utility model, the first elastic piece and the second elastic piece are arranged between the cell culture bottles, so that the cell culture bottles are prevented from being mutually collided during water control, and further are broken;
3. according to the utility model, the water pan is arranged, so that each limiting hole can be aligned, and then the opening of each culture bottle is aligned, the defect that water flows on the ground or on the cell culture bottle at the lower layer is avoided, and the purposes of neat and orderly places and clean cell culture bottles are achieved.
Drawings
FIG. 1 is a top view of the present utility model;
FIG. 2 is a side view of the present utility model;
fig. 3 is a bottom view of the present utility model.
Wherein: 1. a fence frame; 2. a first horizontal bar; 3. a second horizontal bar; 4. a first elastic member; 5. a second elastic member; 6. a limiting plate; 7. a limiting hole; 8. a water receiving frame; 9. a water receiving cross bar; 10. a water receiving longitudinal rod; 11. a water receiving tray; 12. and supporting the vertical rod.
Detailed Description
The utility model will be described in detail below with reference to the drawings and the detailed description.
In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present utility model, unless otherwise indicated, the meaning of "a plurality" is two or more.
The utility model discloses a bracket for placing a cell culture bottle and controlling water, which is shown in figures 1-3 and comprises a surrounding frame 1, a first elastic piece 4, a second elastic piece 5 and a limiting plate 6.
Enclose and keep off frame 1 level setting, enclose and keep off frame 1 by two first horizon bars 2 and two second horizon bars 3 in proper order head and the tail fixed connection form, two first horizon bars 2 set up relatively, two second horizon bars 3 set up relatively, and with the trend of first horizon bar 2 perpendicular.
The first elastic piece 4 is arranged between the two first horizontal rods 2 and is perpendicular to the trend of the first horizontal rods 2, one end of the first elastic piece 4 is fixedly connected with one first horizontal rod 2, and the other end of the first elastic piece 4 is fixedly connected with the other first horizontal rod 2.
The second elastic piece 5 is arranged between the two second horizontal rods 3 and is perpendicular to the trend of the second horizontal rods 3, one end of the second elastic piece 5 is fixedly connected with one second horizontal rod 3, the other end of the second elastic piece 5 is fixedly connected with the other second horizontal rod 3, the second elastic piece 5 is used for forming an elastic accommodating cavity in an interweaving fit with the first elastic piece 4, and the accommodating cavity is used for accommodating a cell culture bottle.
Limiting plate 6 level sets up, and limiting plate 6 is located the below that encloses fender frame 1, has seted up spacing hole 7 on the limiting plate 6, and spacing hole 7 is used for when need controlling water to the cell culture bottle for the opening of cell culture bottle is downwards and its neck stretches into in spacing hole 7 and then controls water to the cell culture bottle, and limiting plate 6 is used for when needs accomodate the cell culture bottle, supports the bottom of cell culture bottle.
The limiting plate 6 is provided with a plurality of limiting holes 7, the first elastic piece 4 and the second elastic piece 5 are also provided with a plurality of limiting holes, a plurality of accommodating cavities are further formed in a surrounding mode, and the first elastic piece 4 and the second elastic piece 5 are further used for preventing two culture bottles in adjacent accommodating cavities from colliding when the openings of the cell culture bottles face upwards or downwards, so that damage to the culture bottles is avoided.
The periphery of each accommodating cavity is surrounded by the first elastic piece 4 and the second elastic piece 5, the vertical axis of each accommodating cavity coincides with the axis of the corresponding limiting hole 7, and the first elastic piece 4 or the second elastic piece 5 between two adjacent accommodating cavities are not shared.
The utility model also includes: the water receiving mechanism, the water receiving mechanism includes: the water receiving frame 8, the water receiving cross bar 9, the water receiving vertical bar 10 and the water receiving tray 11.
The water receiving frame 8 is horizontally arranged, the water receiving frame 8 is formed by encircling four third horizontal rods in a surrounding mode through mutual fixed connection from head to tail, the water receiving cross rod 9 is horizontally arranged, two ends of the water receiving cross rod 9 are respectively fixed on the two oppositely arranged third horizontal rods, the water receiving longitudinal rod 10 is horizontally arranged, and two ends of the water receiving longitudinal rod 10 are respectively fixed on the other two oppositely arranged third horizontal rods.
The water pan 11 is fixed in the crossing department of water receiving horizontal pole 9 and water receiving vertical pole 10, and the axis coincidence of water pan 11 and spacing hole 7, water pan 11 are used for receiving the water that the cell culture bottle flows when controlling water. The water receiving disc 11 is communicated with underground water through a flow guiding pipe.
The utility model also includes: four support pole setting 12, four support pole setting 12 vertical settings, four support pole setting 12 upper ends and enclose fender frame 1 fixed connection, four support pole setting 12 middle part respectively with limiting plate 6 and water receiving frame 8 fixed connection, four support pole setting 12 lower extreme is used for supporting at ground, then supports whole accuse water support.
The foregoing description of the preferred embodiments of the utility model is not intended to limit the utility model to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the utility model are intended to be included within the scope of the utility model.
Claims (6)
1. A rack for holding and controlling water in a cell culture flask, comprising:
the enclosure frame (1) is horizontally arranged and is formed by fixedly connecting two first horizontal rods (2) and two second horizontal rods (3) in sequence from beginning to end, the two first horizontal rods (2) are oppositely arranged, the two second horizontal rods (3) are oppositely arranged and are vertical to the trend of the first horizontal rods (2),
a first elastic piece (4) which is arranged between the two first horizontal rods (2) and is vertical to the trend of the first horizontal rods (2), one end of the first elastic piece is fixedly connected with one first horizontal rod (2), the other end of the first elastic piece is fixedly connected with the other first horizontal rod (2),
the second elastic piece (5) is arranged between the two second horizontal rods (3) and is vertical to the trend of the second horizontal rods (3), one end of the second elastic piece is fixedly connected with one second horizontal rod (3), the other end of the second elastic piece is fixedly connected with the other second horizontal rod (3) and is used for forming an elastic accommodating cavity in an interweaving fit with the first elastic piece (4), the accommodating cavity is used for accommodating a cell culture bottle,
limiting plate (6), the level sets up, is located enclose the below that keeps off frame (1), offered spacing hole (7) on it, spacing hole (7) are used for when need controlling water to the cell culture bottle for the opening of cell culture bottle is downwards and its neck stretches into spacing hole (7) in and then controls water to the cell culture bottle, limiting plate (6) are used for when need accomodating the cell culture bottle, support the bottom of cell culture bottle.
2. The bracket for placing and controlling the water of the cell culture flask according to claim 1, wherein a plurality of limiting holes (7) are formed in the limiting plate (6), a plurality of first elastic pieces (4) and second elastic pieces (5) are also arranged, a plurality of accommodating cavities are further formed by enclosing, and the first elastic pieces (4) and the second elastic pieces (5) are further used for preventing two culture flasks in the adjacent accommodating cavities from collision when the opening of the cell culture flask faces upwards or downwards, so that the damage of the culture flask is avoided.
3. A support for placing and controlling water in a cell culture flask according to claim 2, wherein the periphery of each accommodating cavity is surrounded by a first elastic member (4) and a second elastic member (5), the vertical axis of each accommodating cavity coincides with the axis of the corresponding limiting hole (7), and the first elastic member (4) or the second elastic member (5) between two adjacent accommodating cavities is not shared.
4. The rack for holding and controlling water in a cell culture flask according to claim 1, further comprising: a water receiving mechanism is arranged on the water tank,
the water receiving mechanism comprises:
the water receiving frame (8) is horizontally arranged and is formed by fixedly connecting four third horizontal rods end to end in sequence,
a water receiving cross rod (9) which is horizontally arranged, two ends of the water receiving cross rod are respectively fixed on two third horizontal rods which are oppositely arranged,
a water receiving longitudinal rod (10) which is horizontally arranged, two ends of the water receiving longitudinal rod are respectively fixed on the other two third horizontal rods which are oppositely arranged,
the water receiving disc (11) is fixed at the intersection of the water receiving cross rod (9) and the water receiving longitudinal rod (10), the axis of the water receiving disc is coincident with the axis of the limiting hole (7) and is used for receiving water flowing out of the cell culture flask during water control.
5. A rack for holding and controlling the flow of cell culture flasks according to claim 4, wherein each of said trays (11) is in communication with the groundwater through a flow-guide tube.
6. The rack for holding and controlling water in a cell culture flask according to claim 5, further comprising: four support pole setting (12), vertical setting, its upper end and enclose fender frame (1) fixed connection, its middle part respectively with limiting plate (6) and water receiving frame (8) fixed connection, its lower extreme is used for supporting at ground, then supports whole accuse water support.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320877976.1U CN219546950U (en) | 2023-04-19 | 2023-04-19 | Support for placing cell culture bottle and controlling water of cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320877976.1U CN219546950U (en) | 2023-04-19 | 2023-04-19 | Support for placing cell culture bottle and controlling water of cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219546950U true CN219546950U (en) | 2023-08-18 |
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ID=87736900
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320877976.1U Active CN219546950U (en) | 2023-04-19 | 2023-04-19 | Support for placing cell culture bottle and controlling water of cell culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219546950U (en) |
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2023
- 2023-04-19 CN CN202320877976.1U patent/CN219546950U/en active Active
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