CN219489981U - Multilayer tissue inoculation culture dish - Google Patents
Multilayer tissue inoculation culture dish Download PDFInfo
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- CN219489981U CN219489981U CN202320051554.9U CN202320051554U CN219489981U CN 219489981 U CN219489981 U CN 219489981U CN 202320051554 U CN202320051554 U CN 202320051554U CN 219489981 U CN219489981 U CN 219489981U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The utility model discloses a multilayer tissue inoculation culture dish which comprises a culture dish body and at least one culture plate, wherein the culture dish body is hollow, and the at least one culture plate is sequentially arranged in the culture dish body along the height direction; at least one supporting part is sequentially arranged on the inner peripheral side of the culture dish body along the height direction, and the culture plate is arranged on the supporting part; a plurality of first culture tanks for inoculating tissues are arranged on the bottom surface of the culture dish body; the upper end face and the lower end face of the culture plate are respectively provided with a plurality of culture tank groups for inoculating tissues, each culture tank group comprises a plurality of second culture tanks and a plurality of communication tanks, the plurality of second culture tanks are sequentially arranged at intervals along the transverse direction of the culture dish body, one communication tank is arranged between every two adjacent second culture tanks of each culture tank group, the communication tanks are communicated with the adjacent second culture tanks, and the sizes of the communication tanks are different from those of the second culture tanks; the culture dish body is internally provided with a culture solution for culturing tissues, and the culture solution is used for the culture plate at the uppermost part.
Description
Technical Field
The utility model belongs to the technical field of culture dishes, and particularly relates to a multilayer tissue inoculation culture dish.
Background
A culture dish is a laboratory vessel for the cultivation of microorganisms or cells, typically made of glass or plastic. The materials of the culture dishes are basically divided into two types, mainly plastic and glass, and the glass can be used for plant materials, microorganism culture and adherent culture of animal cells. The plastic may be polyethylene material, has disposable and multiple use, is suitable for laboratory inoculation, streaking and bacteria separation, and may be used in plant material cultivation.
The utility model patent with application number 201410663746.0 discloses a tissue block method culture dish, which consists of a culture dish bottom plate, a culture dish top cover and a porous cover; the culture dish chassis consists of a chassis bottom surface, a chassis side surface and an 8-shaped bracket; the 8-shaped bracket comprises a bracket protruding part, a bracket connecting part and a bracket tubular part, wherein the bracket tubular part is of a hollow tubular structure, the lower surface of the bracket tubular part is provided with a rectangular protrusion, and the edge of the porous cover is provided with a notch. The culture dish effectively solves the problem of difficult adherence in the tissue block culture process, reduces the risk of tissue block pollution, improves the success rate of the tissue block culture method, and can not influence the tissue block culture by long-distance transfer of the culture dish.
The utility model patent with application number 201611179071.8 discloses a biological culture dish, which comprises a round culture dish, wherein the upper wall of the culture dish is provided with an azimuth mark; a circular connecting disc is arranged at the center of the culture dish, a plurality of grooves are uniformly distributed inwards on the circumference of the connecting disc, and a circular truncated cone with the same center is arranged above the center of the connecting disc; the groove is adapted to a card arranged at one end side of the partition plate, the partition plate is clamped on the connecting disc, the partition plate is strip-shaped and consists of a slat with a solid upper part and comb teeth uniformly distributed and transversely arranged at the lower part of the slat, and a through slot is arranged between every two adjacent comb teeth; the card is arranged at the joint of the slat and the comb teeth; the split type design is adopted between the culture dish and the connecting disc and between the connecting disc and the partition plate. Is mainly suitable for culturing microorganisms such as bacteria and the like or animal and plant tissues in a biological laboratory.
The culture dish in the prior art is low in overall height and is of a single-layer structure, more primary cells are required to be harvested, the number of tissue inoculated at the bottom of each dish is required to be increased, the number of culture dishes is required to be increased or the culture dishes are required to be replaced to be larger, the method can increase the material cost, the consumption of culture solution is large, the occupied area is large, and the use efficiency is low.
Disclosure of Invention
Aiming at the problems in the background art, the utility model aims to provide a multi-layer tissue inoculation culture dish which comprises a culture dish body and at least one culture plate, wherein the culture dish body is hollow, and at least one culture plate is sequentially arranged in the culture dish body along the height direction;
at least one supporting part is sequentially arranged on the inner peripheral side of the culture dish body along the height direction, and the culture plate is arranged on the supporting part; a plurality of first culture tanks for inoculating tissues are arranged on the bottom surface of the culture dish body;
a plurality of culture tank groups for inoculating tissues are arranged on the upper end face and the lower end face of the culture plate, each culture tank group comprises a plurality of second culture tanks and a plurality of communication tanks, the second culture tanks are sequentially arranged at intervals along the transverse direction of the culture dish body, one communication tank is arranged between every two adjacent second culture tanks of each culture tank group, the communication tanks are communicated with the adjacent second culture tanks, and the sizes of the communication tanks are different from those of the second culture tanks;
the culture dish is characterized in that a culture solution for culturing tissues is arranged in the culture dish body, and the culture solution is used for submerging the uppermost culture plate.
Preferably, the support part is a support step, and the support step is arranged on one circle of the inner peripheral side of the culture dish body.
Preferably, the two support steps are a first support step and a second support step, the second support step is located above the first support step, and the width of the second support step is larger than that of the first support step;
the culture plates are two, namely a first culture plate and a second culture plate, the size of the second culture plate is larger than that of the first culture plate, the first culture plate is arranged on the first support step, and the second culture plate is arranged on the second support step.
Preferably, the number and the positions of the culture groove groups on the upper end face and the lower end face of the culture plate are in one-to-one correspondence and are communicated with each other in the height direction, so that an integrated through groove is formed between the second culture grooves corresponding to the upper end face and the lower end face of the culture plate and between the communication grooves corresponding to each other.
Preferably, a plurality of culture tank groups are sequentially arranged at intervals along the longitudinal direction of the culture dish body.
Preferably, the first culture tank and the second culture tank are both circular, and the width of the communicating tank is smaller than the diameter of the second culture tank.
Preferably, a plurality of the first culture tanks are arranged along the transverse and longitudinal arrays of the culture dish body.
Preferably, the culture dish body and the culture plate are made of glass or plastic.
By adopting the technical scheme, the utility model has the following advantages and positive effects compared with the prior art:
1. according to the utility model, at least one culture plate is arranged in the culture dish body, so that tissues can be cultivated on both the culture dish body and the culture plate, more tissues can be inoculated in a limited space, the amount of inoculated tissue blocks is increased, and more primary cells cultivated by the tissues are harvested. And all be equipped with a plurality of culture tank groups that are used for inoculation tissue on the up end and the lower terminal surface of culture plate for every culture plate front and back face can both climb out the tissue cell, can gather more cells on the unit area, saves cost more, availability factor is high, the tissue utilization rate is high.
2. The culture tank group comprises a plurality of second culture tanks and a plurality of communicating tanks, the plurality of second culture tanks are sequentially arranged at intervals along the transverse direction of the culture dish body, one communicating tank is arranged between every two adjacent second culture tanks of each culture tank group, the communicating tanks are communicated with the adjacent second culture tanks, on one hand, the up-and-down flow of culture fluid can be facilitated through the arrangement of the communicating tanks, on the other hand, tissues can be inoculated in the communicating tanks, the size of the communicating tanks is different from that of the second culture tanks, so that tissues with different sizes can be inoculated, meanwhile, the second culture tanks can be combined with the communicating tanks to cultivate special-shaped tissues, that is to say, the special-shaped tissues with different sizes at two ends can be combined to cultivate, one end of each special-shaped tissue is clamped in the second culture tank, and the other end of each special-shaped tissue is clamped in the communicating tank, so that the whole applicability is stronger.
Drawings
The utility model is described in further detail below with reference to the attached drawing figures, wherein:
FIG. 1 is a cross-sectional perspective view of the present utility model;
FIG. 2 is a perspective view of the culture dish body of the present utility model;
FIG. 3 is a schematic view of a first plate of the present utility model;
FIG. 4 is a schematic view of a second plate according to the present utility model.
Reference numerals illustrate:
1: a culture dish body; 2: a first culture plate; 3: a second culture plate; 4: a first support step; 5: a second support step; 6: a first culture tank; 7: a culture tank group; 8: a second culture tank; 9: and a communication groove.
Detailed Description
The utility model is described in further detail below with reference to the drawings and the specific examples. The advantages and features of the present utility model will become more apparent from the following description. It is noted that the drawings are in a very simplified form and utilize non-precise ratios, and are intended to facilitate a convenient, clear, description of the embodiments of the utility model.
It should be noted that all directional indicators (such as up, down, left, right, front, and rear … …) in the embodiments of the present utility model are merely used to explain the relative positional relationship, movement, etc. between the components in a particular posture (as shown in the drawings), and if the particular posture is changed, the directional indicator is changed accordingly.
With reference to fig. 1 to 4, the core of the present utility model is to provide a multi-layered tissue inoculation culture dish, which comprises a culture dish body 1 and at least one culture plate, wherein the interior of the culture dish body 1 is hollow, and the at least one culture plate is sequentially arranged in the culture dish body 1 along the height direction.
The culture dish body 1 in this embodiment has a regular cube shape, but may have a rectangular parallelepiped shape, a cylindrical shape, or the like in other embodiments, and is not limited thereto.
A plurality of first culture tanks 6 for inoculating tissues are arranged on the bottom surface of the culture dish body 1, the first culture tanks 6 are round, and the plurality of first culture tanks 6 are arranged along the transverse and longitudinal arrays of the culture dish body 1. When in inoculation, the tissue is clamped in the first culture tank 6, so that the tissue is tightly clamped on the wall of the first culture tank 6, and the tissue can be adhered to the wall of the first culture tank 6 after being dried, thus completing inoculation.
The culture dish body 1 is equipped with at least one supporting part in proper order along the direction of height in inside week side, and the culture plate is located on the supporting part. Specifically, the supporting part is a supporting step, and the supporting step is arranged on one circle of the inner circumference side of the culture dish body 1.
In this embodiment, two support steps are provided, which are a first support step 4 and a second support step 5, respectively, the second support step 5 is located above the first support step 4, and the width of the second support step 5 is greater than that of the first support step 4. The culture plate is equipped with two, is first culture plate 2 and second culture plate 3 respectively, and the size of second culture plate 3 is greater than first culture plate 2, and first culture plate 2 is located on first support step 4, and second culture plate 3 is located on the second support step 5.
A plurality of culture groove groups 7 for inoculating tissues are arranged on the upper end face and the lower end face of the culture plate, and the culture groove groups 7 are sequentially arranged at intervals along the longitudinal direction of the culture dish body 1. The culture tank group 7 comprises a plurality of second culture tanks 8 and a plurality of communication tanks 9, the plurality of second culture tanks 8 are arranged at intervals along the transverse direction of the culture dish body 1, a communication tank 9 is arranged between every two adjacent second culture tanks 8 of the culture tank group 7, the communication tanks 9 are communicated with the adjacent second culture tanks 8, and the size of the communication tanks 9 is different from that of the second culture tanks 8. The second culture tanks 8 are all circular, and the width of the communicating tank 9 is smaller than the diameter of the second culture tanks 8.
The culture dish body 1 is internally provided with a culture solution for culturing tissues, and the culture solution is used for submerging the uppermost second culture plate 3.
According to the utility model, at least one culture plate is arranged in the culture dish body 1, so that tissues can be cultivated on both the culture dish body 1 and the culture plate, more tissues can be inoculated in a limited space, the amount of inoculated tissue blocks is increased, and more primary cells cultivated by the tissues are harvested. And all be equipped with a plurality of culture tank groups 7 that are used for inoculation tissue on the up end and the lower terminal surface of culture plate for every culture plate front and back face can both climb out the tissue cell, can gather more cells on the unit area, saves cost more, availability factor is high, the tissue utilization rate is high.
The culture tank group 7 comprises a plurality of second culture tanks 8 and a plurality of communication tanks 9, the plurality of second culture tanks 8 are sequentially arranged at intervals along the transverse direction of the culture dish body 1, one communication tank 9 is arranged between every two adjacent second culture tanks 8 of each culture tank group 7, the communication tanks 9 are communicated with the adjacent second culture tanks 8, on one hand, the up-and-down flow of culture fluid can be facilitated through the arrangement of the communication tanks 9, on the other hand, tissues can be inoculated in the communication tanks 9, the sizes of the communication tanks 9 are different from those of the second culture tanks 8, so that tissues with different sizes can be inoculated, meanwhile, the second culture tanks 8 and the communication tanks 9 can be combined to cultivate special-shaped tissues with different sizes at two ends, that is to say, one end of each special-shaped tissue is clamped in the second culture tank 8, and the other end of each special-shaped tissue is clamped in the communication tank 9, and the whole applicability is stronger.
Further, the number and the positions of the culture tank groups 7 of the upper end face and the lower end face of the culture plate are in one-to-one correspondence and are communicated with each other in the height direction, so that an integrated through groove is formed between the second culture tanks 8 of which the upper end face and the lower end face are mutually corresponding and between the communication tanks 9 of which the upper end face and the lower end face are mutually corresponding. That is to say, the culture tank of culture plate up end and lower terminal surface is integrated into one piece structure, establishes the tissue card at the upper and lower both ends of second culture tank 8 and the intercommunication groove 9 of culture tank during inoculation for the tissue hugs closely the card and establishes on the cell wall of second culture tank 8 and intercommunication groove 9, can adhere on the cell wall of second culture tank 8 and intercommunication groove 9 after the tissue dries, accomplishes the inoculation promptly. Of course, in other embodiments, the culture tanks on the upper end surface and the lower end surface of the culture plate can also be in split structures, and the culture tanks are respectively blind tanks, which are not limited herein.
Preferably, the culture dish body 1 and the culture plate are made of glass or plastic, such as high boron silicon, polystyrene, polypropylene, etc.
The inoculation and cultivation process of the present utility model is further described below:
1. after washing various tissues, the tissues are cut into small pieces.
2. The tissue is uniformly clamped in the first culture tank 6, and after standing and airing, the tissue is adhered in the first culture tank 6, and a little culture solution is added into the culture dish body 1.
3. The upper and lower ends of the second culture tank 8 and the communication tank 9 of the first culture plate 2 and the second culture plate 3 are sequentially inoculated with tissues, then the first culture plate 2 and the second culture plate 3 are sequentially put into the culture dish body 1, and then the culture solution is slowly added to the culture dish body, so that the second culture plate 3 at the uppermost surface is not used.
The culture solution is used for wetting all tissues by the uppermost second culture plate 3, oxygen in the culture dish body 1 (95% of air in the conventional culture dish body 1) is gradually dissolved in the culture solution for tissue respiration, the respiration of tissue cells cultured in vitro is generally glycolysis, low-concentration oxygen is needed in the environment where the tissue cells grow, and the oxygen dissolved in the culture solution is sufficient for the respiration of tissues.
According to the utility model, more tissues can be inoculated in the limited space of the culture dish body 1, the space utilization rate is high, and the primary culture of the tissues can be efficiently carried out.
The embodiments of the present utility model have been described in detail with reference to the drawings, but the present utility model is not limited to the above embodiments. Even if various changes are made to the present utility model, it is within the scope of the appended claims and their equivalents to fall within the scope of the utility model.
Claims (8)
1. The multilayer tissue inoculation culture dish is characterized by comprising a culture dish body and at least one culture plate, wherein the culture dish body is hollow, and at least one culture plate is sequentially arranged in the culture dish body along the height direction;
at least one supporting part is sequentially arranged on the inner peripheral side of the culture dish body along the height direction, and the culture plate is arranged on the supporting part; a plurality of first culture tanks for inoculating tissues are arranged on the bottom surface of the culture dish body;
a plurality of culture tank groups for inoculating tissues are arranged on the upper end face and the lower end face of the culture plate, each culture tank group comprises a plurality of second culture tanks and a plurality of communication tanks, the second culture tanks are sequentially arranged at intervals along the transverse direction of the culture dish body, one communication tank is arranged between every two adjacent second culture tanks of each culture tank group, the communication tanks are communicated with the adjacent second culture tanks, and the sizes of the communication tanks are different from those of the second culture tanks;
the culture dish is characterized in that a culture solution for culturing tissues is arranged in the culture dish body, and the culture solution is used for submerging the uppermost culture plate.
2. The multi-layered tissue-inoculating culture dish according to claim 1, wherein the supporting part is a supporting step provided at one turn of the inner peripheral side of the culture dish body.
3. The multi-layered tissue-inoculating culture dish according to claim 2, wherein two support steps are provided, namely a first support step and a second support step, the second support step is located above the first support step, and the width of the second support step is larger than that of the first support step;
the culture plates are two, namely a first culture plate and a second culture plate, the size of the second culture plate is larger than that of the first culture plate, the first culture plate is arranged on the first support step, and the second culture plate is arranged on the second support step.
4. The multi-layered tissue culture dish according to claim 1, wherein the culture tank groups of the upper and lower end surfaces of the culture plate are in one-to-one correspondence in number and position and are communicated with each other in a height direction, so that an integrated through groove is formed between the second culture tanks of the upper and lower end surfaces of the culture plate which are in correspondence with each other and between the communication tanks which are in correspondence with each other.
5. The multi-layered tissue-inoculating culture dish of claim 1, wherein a plurality of said culture tank groups are sequentially arranged at intervals in the longitudinal direction of said culture dish body.
6. The multi-layered tissue-inoculating culture dish of claim 1, wherein said first culture tank and said second culture tank are each circular, and wherein the width of said communication tank is smaller than the diameter of said second culture tank.
7. The multi-layered tissue-seeding culture dish according to claim 1, wherein a plurality of the first culture tanks are arranged along a lateral and longitudinal array of the culture dish body.
8. The multi-layered tissue-seeding culture dish according to claim 1, wherein the culture dish body and the culture plate are made of glass or plastic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320051554.9U CN219489981U (en) | 2023-01-09 | 2023-01-09 | Multilayer tissue inoculation culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320051554.9U CN219489981U (en) | 2023-01-09 | 2023-01-09 | Multilayer tissue inoculation culture dish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219489981U true CN219489981U (en) | 2023-08-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320051554.9U Active CN219489981U (en) | 2023-01-09 | 2023-01-09 | Multilayer tissue inoculation culture dish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219489981U (en) |
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2023
- 2023-01-09 CN CN202320051554.9U patent/CN219489981U/en active Active
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