CN219449647U - Series fermentation system for artificial pit mud 3-level seed spread cultivation - Google Patents
Series fermentation system for artificial pit mud 3-level seed spread cultivation Download PDFInfo
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- CN219449647U CN219449647U CN202320604372.XU CN202320604372U CN219449647U CN 219449647 U CN219449647 U CN 219449647U CN 202320604372 U CN202320604372 U CN 202320604372U CN 219449647 U CN219449647 U CN 219449647U
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- 238000000855 fermentation Methods 0.000 title claims abstract description 92
- 230000004151 fermentation Effects 0.000 title claims abstract description 89
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 75
- 238000009826 distribution Methods 0.000 claims abstract description 13
- 239000000498 cooling water Substances 0.000 claims description 14
- 239000000463 material Substances 0.000 claims description 12
- 239000011521 glass Substances 0.000 claims description 5
- 229910001220 stainless steel Inorganic materials 0.000 claims description 4
- 239000010935 stainless steel Substances 0.000 claims description 4
- 238000011218 seed culture Methods 0.000 abstract description 14
- 239000007788 liquid Substances 0.000 abstract description 11
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 4
- 238000003756 stirring Methods 0.000 abstract description 4
- 239000001569 carbon dioxide Substances 0.000 abstract description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 abstract description 3
- 238000005265 energy consumption Methods 0.000 abstract description 3
- 239000007789 gas Substances 0.000 description 10
- 239000001963 growth medium Substances 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 7
- 244000005700 microbiome Species 0.000 description 6
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 4
- 239000000919 ceramic Substances 0.000 description 4
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 4
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 125000003118 aryl group Chemical group 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 230000001476 alcoholic effect Effects 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 235000015096 spirit Nutrition 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model belongs to the technical field of biological fermentation, and particularly relates to a serial fermentation system for artificial pit mud 3-level seed spread cultivation. The utility model relates to a serial fermentation system for artificial pit mud 3-level seed expansion cultivation, which comprises an alcohol fermentation tank and a 3-level seed tank, wherein the bottom of the alcohol fermentation tank is provided with an alcohol tank discharge port, and the top of the alcohol fermentation tank is provided with a safety valve, an alcohol tank feed port and an alcohol tank exhaust port; the top of the 3-level seed tank is provided with a seed tank exhaust port and a seed tank feed port, the bottom of the 3-level seed tank is provided with a seed tank discharge port, the bottom of the 3-level seed tank is provided with a gas distribution ring, and the gas distribution ring is connected with the alcohol tank exhaust port through a pipeline. The utility model has good fermentation quality and high efficiency, is convenient to operate, can monitor the environment in the tank in real time through the thermometer and the pressure gauge, can utilize the carbon dioxide gas generated by the alcohol fermentation tank to stir and mix the fermentation liquid of the 3-level seed tank uniformly, saves energy consumption, reduces labor cost, can ensure the anaerobic fermentation environment in the 3-level seed tank, and improves the seed culture effect.
Description
Technical Field
The utility model belongs to the technical field of biological fermentation, and particularly relates to a serial fermentation system for artificial pit mud 3-level seed spread cultivation.
Background
The strong aromatic Chinese spirits are brewed by using mud pits, so pit mud has very important influence on the quality of the strong aromatic Chinese spirits. The pit mud contains abundant microorganisms, mainly caproic acid bacteria, butyric acid bacteria, methane oxidizing bacteria, propionic acid bacteria, thermophilic bacillus and the like, and fermentation metabolites of the microorganisms are the main sources of aroma substances in the strong aromatic white spirit. The artificial pit mud is prepared by performing enlarged culture on microorganisms in the pit mud by using a modern fermentation technology, and simulating a microorganism system in a natural old pit. The artificial pit mud is applied to the newly-built pit, so that the ageing of the newly-built pit can be promoted, and the quality of the Luzhou-flavor liquor is improved.
The production process of the artificial pit mud comprises the following steps: high-quality old pit mud strain enrichment, inoculation into a 1-level seed culture medium (2500 mL triangular flask), culture at 37 ℃ for 8 days, 10% inoculation into a 2-level seed culture medium (25L plastic barrel), culture at 35-37 ℃ for 8 days, 5% inoculation into a 3-level seed culture medium (500L pottery jar), culture at 30-35 ℃ for 8-10 days, adding 3-level seeds into pit mud prepared based on yellow clay, uniformly stirring, covering with a plastic film, and culturing in a fermentation tank for 30 days.
The 3-level seed culture container is a ceramic jar, and the culture medium comprises corn alcohol distiller's grains, bean pulp powder, alcohol, white spirit distiller's grains, yeast and dipotassium hydrogen phosphate. Cleaning ceramic jar, spraying alcohol, sterilizing, weighing raw materials according to the formula of 3-stage seed culture medium, adding into the ceramic jar, adding 100deg.C hot water, cooling to below 40deg.C, inoculating 5% of cultured secondary seed liquid, and standing at 30-35deg.C for 8-10 days.
The existing artificial pit mud 3-level seed spread cultivation technology has the following problems: (1) The container for culturing the 3-level seeds is a ceramic jar, and the container is low in price, cannot be sterilized at high temperature, is easy to crush and cannot control the temperature; (2) In the actual culture process, microorganisms and solid nutrient substances in the culture medium are easy to settle at the bottom of the jar, are unevenly distributed, and influence the expanding culture effect; (3) In order to uniformly distribute substances in the culture medium, a wood rake is used for stirring, so that oxygen is brought into the seed liquid to destroy the formed anaerobic fermentation environment, and the proliferation of anaerobic bacteria such as caproic acid bacteria, butyric acid bacteria and methane bacteria is not facilitated.
Disclosure of Invention
In order to solve the technical problems, the utility model provides a serial fermentation system for artificial pit mud 3-level seed spread cultivation, which has good fermentation quality, high efficiency and simple and convenient operation.
The utility model relates to a serial fermentation system for artificial pit mud 3-level seed expansion cultivation, which comprises an alcohol fermentation tank and a 3-level seed tank, wherein the bottom of the alcohol fermentation tank is provided with an alcohol tank discharge port, and the top of the alcohol fermentation tank is provided with a safety valve, an alcohol tank feed port and an alcohol tank exhaust port; the top of the 3-level seed tank is provided with a seed tank exhaust port and a seed tank feed port, the bottom of the 3-level seed tank is provided with a seed tank discharge port, the bottom of the 3-level seed tank is provided with a gas distribution ring, and the gas distribution ring is connected with the alcohol tank exhaust port through a pipeline.
Preferably, the gas distribution ring is an annular hollow pipeline provided with a plurality of through holes.
Preferably, the outer walls of the alcohol fermentation tank and the 3-level seed tank are respectively provided with a jacket, the outer walls are connected in series through a pipeline, the jacket of the alcohol fermentation tank is provided with a cooling water inlet, and the jacket of the 3-level seed tank is provided with a cooling water outlet.
Preferably, the outer walls of the alcohol fermentation tank and the 3-level seed tank are provided with sight glass, and the fermentation condition in the tank can be observed through the sight glass.
Preferably, the alcohol fermentation tank and the 3-level seed tank are both provided with a thermometer and a pressure gauge, and the fermentation temperature and the pressure in the tank are monitored in real time.
Preferably, the 3-stage seed tank volume is greater than the alcohol fermenter volume.
Preferably, the materials of the alcohol fermentation tank and the grade 3 seed tank are stainless steel.
Preferably, the material inlet of the alcohol tank and the material inlet of the seed tank are used for feeding, and can be used for overhauling.
Compared with the prior art, the utility model has the beneficial effects that:
1. the utility model has good fermentation quality and high efficiency, is convenient to operate, can monitor the environment in the tank in real time through the thermometer and the pressure gauge, can utilize the carbon dioxide gas generated by the alcohol fermentation tank to stir and mix the fermentation liquid of the 3-level seed tank uniformly, saves energy consumption, reduces labor cost, can ensure the anaerobic fermentation environment in the 3-level seed tank, and improves the seed culture effect;
2. the two fermentation tanks are made of stainless steel, meet the food sanitation standard, have smooth inner walls, are convenient to clean and high in structural strength, and can be used for directly sterilizing culture mediums in the tanks;
3. the jackets are arranged on the outer walls of the two fermentation tanks, so that the temperature can be controlled; the jacket cooling water of the two tanks is connected in series, and the fermentation temperature of the alcohol fermentation tank is lower, the fermentation temperature of the 3-level seed tank is higher, so that the consumption of the cooling water can be reduced and the energy consumption is reduced through the series connection;
4. the alcoholic fermentation liquid generated after the fermentation of the alcoholic fermentation tank can be used as the main raw material of the 3-level seed culture medium, and the corn alcohol vinasse, alcohol and yeast in the original seed culture medium are not needed to be added, so that the production cost is reduced.
Drawings
FIG. 1 is a schematic structural diagram of a serial fermentation system for artificial pit mud 3-level seed propagation;
in the figure: 1. an alcohol fermenter; 2. a 3-stage seed tank; 3. a discharge hole of the alcohol tank; 4. a safety valve; 5. a material inlet of the alcohol tank; 6. an exhaust port of the alcohol tank; 7. an exhaust port of the seed tank; 8. a feed port of the seed tank; 9. a seed tank discharge port; 10. a gas distribution ring; 11. a jacket; 12. a cooling water inlet; 13. a cooling water outlet; 14. a viewing mirror; 15. a thermometer; 16. a pressure gauge.
Detailed Description
The utility model will be further described with reference to the accompanying drawings.
As shown in figure 1, the serial fermentation system for the artificial pit mud 3-level seed propagation comprises an alcohol fermentation tank 1 and a 3-level seed tank 2, wherein an alcohol tank discharge port 3 is arranged at the bottom of the alcohol fermentation tank 1, and a safety valve 4, an alcohol tank feed port 5 and an alcohol tank exhaust port 6 are arranged at the top of the alcohol fermentation tank 1; the top of the 3-level seed tank 2 is provided with a seed tank exhaust port 7 and a seed tank feed port 8, the bottom of the 3-level seed tank is provided with a seed tank discharge port 9, the bottom of the 3-level seed tank is provided with a gas distribution ring 10, and the gas distribution ring 10 is connected with the alcohol tank exhaust port 6 through a pipeline.
The gas distribution ring 10 is an annular hollow pipe, and is provided with a plurality of through holes.
The outer walls of the alcohol fermentation tank 1 and the 3-level seed tank 2 are respectively provided with a jacket 11, the jackets 11 of the alcohol fermentation tank 1 are connected in series through pipelines, the jackets 11 of the 3-level seed tank 2 are provided with cooling water inlets 12, and the jackets 11 of the 3-level seed tank 2 are provided with cooling water outlets 13.
The outer walls of the alcohol fermentation tank 1 and the 3-level seed tank 2 are respectively provided with a sight glass 14, and the fermentation conditions in the tank can be observed through the sight glass 14.
The alcohol fermentation tank 1 and the 3-level seed tank 2 are respectively provided with a thermometer 15 and a pressure meter 16, and the fermentation temperature and the pressure in the tank are monitored in real time.
The volume of the 3-level seed tank 2 is larger than that of the alcohol fermentation tank 1.
The materials of the alcohol fermentation tank 1 and the 3-level seed tank 2 are stainless steel.
The alcohol tank feed inlet 5 and the seed tank feed inlet 8 are used for feeding materials, and can be used for overhauling.
The working process of the utility model comprises the following steps:
during production, the alcohol fermentation tank 1 carries out alcohol fermentation, required materials are put into the alcohol tank through the feed opening 5 for steam sterilization, enzymes required by fermentation are added after the materials are cooled to 60 ℃, and alcohol active dry yeast is added after the materials are cooled to 30 ℃ for alcohol fermentation; meanwhile, 3-level seed tank 2 carries out 3-level seed culture, alcohol fermentation liquid and other materials generated by the previous fermentation of the alcohol fermentation tank 1 are put into a feed port 8 of the seed tank, steam sterilization is carried out, 2-level seed liquid is added when cooling to 35 ℃, and 3-level seed culture is carried out; the cooling of the alcohol fermentation tank 1 and the 3-level seed tank 2 can be performed by introducing cooling water into the jacket 11.
After the fermentation materials of the alcohol fermentation tank 1 and the 3-level seed tank 2 are prepared, heat is generated by starting fermentation, at the moment, cooling water is introduced through a cooling water inlet 12, the temperature is displayed by combining a thermometer 15 through controlling the temperature of the cooling water inlet 12, and the temperature in the alcohol fermentation tank 1 and the 3-level seed tank 2 is regulated and controlled.
In the fermentation process, carbon dioxide gas generated by the alcohol fermentation tank 1 enters the 3-level seed tank 2 through the alcohol tank exhaust port 6 and is dispersed into seed fermentation liquid through the gas distribution ring 10, and the fermentation liquid is stirred and mixed, so that microorganisms and solid nutrient substances in the fermentation liquid are uniformly distributed, and meanwhile, the anaerobic environment inside the 3-level seed tank 2 is maintained, and the seed culture effect is ensured.
After the 3-level seed culture is finished, discharging the seeds from a discharge hole 9 of a seed tank for the next pit mud preparation process; after the fermentation of the alcohol fermentation tank 1 is finished, the alcohol fermentation liquid is discharged from the discharge port 3 of the alcohol tank and can be pumped into the 3-level seed tank 2 again to be used as the raw material for the next 3-level seed culture or used for other purposes.
Claims (7)
1. A3 grades of seed of artifical pit mud expand and cultivate with series connection fermentation system, its characterized in that: the device comprises an alcohol fermentation tank (1) and a 3-level seed tank (2), wherein an alcohol tank discharge port (3) is arranged at the bottom of the alcohol fermentation tank (1), and a safety valve (4), an alcohol tank feed port (5) and an alcohol tank exhaust port (6) are arranged at the top of the alcohol fermentation tank; the top of the 3-level seed tank (2) is provided with a seed tank exhaust port (7) and a seed tank feed port (8), the bottom of the 3-level seed tank is provided with a seed tank discharge port (9), the bottom of the 3-level seed tank is provided with a gas distribution ring (10), and the gas distribution ring (10) is connected with the alcohol tank exhaust port (6) through a pipeline.
2. The serial fermentation system for artificial pit mud grade 3 seed propagation according to claim 1, wherein: the gas distribution ring (10) is an annular hollow pipeline and is provided with a plurality of through holes.
3. The serial fermentation system for artificial pit mud grade 3 seed propagation according to claim 1, wherein: the outer walls of the alcohol fermentation tank (1) and the 3-level seed tank (2) are respectively provided with a jacket (11), the jackets (11) of the alcohol fermentation tank (1) are connected in series through pipelines, the jackets (11) of the 3-level seed tank (2) are provided with cooling water inlets (12), and the jackets (11) of the 3-level seed tank (2) are provided with cooling water outlets (13).
4. The serial fermentation system for artificial pit mud grade 3 seed propagation according to claim 1, wherein: the outer walls of the alcohol fermentation tank (1) and the 3-level seed tank (2) are provided with sight glass (14).
5. The serial fermentation system for artificial pit mud grade 3 seed propagation according to claim 1, wherein: the alcohol fermentation tank (1) and the 3-level seed tank (2) are both provided with a thermometer (15) and a pressure gauge (16).
6. The serial fermentation system for artificial pit mud grade 3 seed propagation according to claim 1, wherein: the volume of the 3-level seed tank (2) is larger than that of the alcohol fermentation tank (1).
7. The serial fermentation system for artificial pit mud grade 3 seed propagation according to claim 1, wherein: the materials of the alcohol fermentation tank (1) and the 3-level seed tank (2) are stainless steel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320604372.XU CN219449647U (en) | 2023-03-24 | 2023-03-24 | Series fermentation system for artificial pit mud 3-level seed spread cultivation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320604372.XU CN219449647U (en) | 2023-03-24 | 2023-03-24 | Series fermentation system for artificial pit mud 3-level seed spread cultivation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219449647U true CN219449647U (en) | 2023-08-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320604372.XU Active CN219449647U (en) | 2023-03-24 | 2023-03-24 | Series fermentation system for artificial pit mud 3-level seed spread cultivation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219449647U (en) |
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2023
- 2023-03-24 CN CN202320604372.XU patent/CN219449647U/en active Active
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240419 Address after: Shou Zhang Ji Zhen Xu Fang Cun, Liangshan County, Jining City, Shandong Province 272600 Patentee after: SHANDONG LIANGSHAN XUFANG DAQU CO.,LTD. Country or region after: China Address before: 273100 No.1 Xingtan Road, Qufu City, Jining City, Shandong Province Patentee before: JINING University Country or region before: China |
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| TR01 | Transfer of patent right |