CN219440348U - Rat bile duct catheter and experimental device with same - Google Patents

Abstract

The embodiment of the utility model provides a rat bile duct catheter, which comprises: a first conduit (1); a single junction (2) connected to the first conduit (1); a second conduit (3) connected to the unijunction (2); a first double nodule (4) connected to the second catheter (3); a third conduit (5) connected to the first double nodule (4); the first catheter (1) is inserted into the common bile duct and fixed through the single nodule (2), the third catheter (5) is inserted into the duodenum and fixed through the first double nodule (4), and bile is taken out from the second catheter (3). According to the embodiment of the utility model, the first catheter is fixed on the side of the common bile duct through the single nodule after being inserted into the common bile duct, the third catheter is fixed on the side of the duodenum through the first double nodule after being inserted into the duodenum, the second catheter is positioned outside the rat body, bile can be taken out from the second catheter, the physiological structure of the rat is met, the fixed catheter is not easy to fall off, the requirement of periodically collecting bile for a long time is met, and the requirement of animal welfare is met.

Description

Rat bile duct catheter and experimental device with same

Technical Field

The utility model relates to the technical field of animal experiments, in particular to a rat bile duct catheter and an experimental device with the same.

Background

In the process of drug development, it is often necessary to periodically collect bile from experimental animals to determine the half-life of the drug in order to determine the efficacy of the drug, conduct a drug toxicological analysis, and the like.

At present, the method for collecting bile of an experimental animal is mainly to extract the bile of the experimental animal in an operation after the anesthesia of the experimental animal. The method has great damage to experimental animals, the experimental animals cannot survive for a long time, the long-time periodic collection is difficult to achieve, and the method does not meet the requirements of animal welfare.

If long-time periodic bile collection is required to be completed in vitro of the experimental animal, the common bile duct and the duodenum of the experimental animal are communicated when bile collection is not performed, and a bile flow channel is constructed, bile duct intubation is an essential operation for the experimental animal. However, the existing bile duct catheter is simple in design and easy to fall off, and the requirement of long-time periodic collection is difficult to meet.

Disclosure of Invention

The embodiment of the utility model provides a rat bile duct catheter, which aims to solve the problem that the bile duct catheter is easy to fall off when bile is periodically collected for a long time by an experimental animal in the prior art.

In a first aspect, there is provided a rat bile duct catheter comprising:

a first conduit (1);

a single junction (2) connected to the first conduit (1);

a second conduit (3) connected to the unijunction (2);

a first double nodule (4) connected to the second catheter (3); and

a third conduit (5) connected to the first double nodule (4);

wherein the first catheter (1) is inserted into the common bile duct and fixed by the single nodule (2), the third catheter (5) is inserted into the duodenum and fixed by the first double nodule (4), and bile is taken out from the second catheter (3).

In a second aspect, there is provided an experimental set-up comprising a rat bile duct as described above, an implanter and a syringe;

the injector is connected with the second catheter (3) through the implantation device, and bile is taken out from the second catheter (3).

In a third aspect, there is provided an experimental set-up comprising a rat bile duct as described above, an implanter and a bile circulator;

the bile circulator is connected with the second catheter (3) through the implantation device to form a closed loop bile flow channel.

According to the embodiment of the utility model, the first catheter is fixed on the side of the common bile duct through the single nodule after being inserted into the common bile duct, the third catheter is fixed on the side of the duodenum through the first double nodule after being inserted into the duodenum, the second catheter is positioned outside the rat body, bile can be taken out from the second catheter, the physiological structure of the rat is met, the fixed catheter is not easy to fall off, the requirement of periodically collecting bile for a long time is met, and the requirement of animal welfare is met.

Drawings

The foregoing and/or additional aspects and advantages of the utility model will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings, in which:

FIG. 1 is a schematic view of a rat bile duct catheter according to an embodiment of the utility model;

FIG. 2 is a schematic diagram of an experimental apparatus provided in a third embodiment of the present utility model;

fig. 3 is a schematic diagram of an experimental apparatus according to a fourth embodiment of the present utility model.

Detailed Description

Embodiments of the present utility model are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar modules or modules having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative only and are not to be construed as limiting the utility model. On the contrary, the embodiments of the utility model include all alternatives, modifications and equivalents as may be included within the spirit and scope of the appended claims.

The method for extracting bile in operation after the anesthesia of the experimental animal requires that the operation is performed on the abdomen of the experimental animal, and then the wound is sutured after the bile is extracted, so that primary bile collection is realized. The method has great damage to the experimental animal, and the experimental animal often can not bear the damage for many times and die, so that the drug effect of the drug in different stages in the same experimental animal can not be measured.

The method for performing bile duct intubation on the experimental animal and periodically collecting bile outside the experimental animal body only needs one operation to embed the bile duct in the experimental animal body, so that bile can be collected outside the experimental animal body for multiple times when the experimental animal is in a waking state; the bile collection is not carried out, and the bile duct and the duodenum of the experimental animal are still communicated, so that a closed-loop bile flow channel is formed, and the normal physiological function of the experimental animal is maintained. The method requires that the influence of the bile duct catheter on the experimental animal is small, the stress response of the experimental animal is reduced as much as possible, and the bile duct catheter can be still fixed on the common bile duct and the duodenum and is not easy to fall off under the condition that the experimental animal carries the bile duct catheter for a long time.

According to the embodiment of the utility model, the first catheter is fixed on the side of the common bile duct through the single nodule after being inserted into the common bile duct, the third catheter is fixed on the side of the duodenum through the first double nodule after being inserted into the duodenum, the second catheter is positioned outside the rat body, bile can be taken out from the second catheter, the physiological structure of the rat is met, the fixed catheter is not easy to fall off, the requirement of periodically collecting bile for a long time is met, and the requirement of animal welfare is met.

Example 1

Fig. 1 is a schematic view of a rat bile duct catheter according to an embodiment of the utility model. As shown in fig. 1, the rat bile duct comprises a first duct 1, a unijunction 2, a second duct 3, a first double nodule 4 and a third duct 5. The first catheter 1, the unijunction 2, the second catheter 3, the first double nodule 4 and the third catheter 5 are connected in sequence, through which bile can flow.

During the feeding process of rats, food enters the duodenum from the stomach, and bile flows into the duodenum from the common bile duct, thereby promoting the digestion of fat in the food. Collecting bile and forming a closed loop bile flow passage requires that a rat bile duct be connected between the common bile duct and the duodenum. Thus, the design of rat bile duct catheters is largely based on a full understanding of the physiological architecture of rats and the stress response of rats.

The depth of the rat bile duct catheter inserted into the common bile duct needs to be proper, the excessive length can cause bile obstruction, and the rat bile duct catheter is easy to fall off when too short; the depth of inserting into the duodenum is also required to be proper, too long can cause intestinal obstruction, too short is easy to fall off, and intestinal tissues are smooth and soft, so that the difficulty of fixation is further increased, and experimental failure is easily caused due to the fact that fixation is not firm. The thickness of each part of the rat bile duct is proper, too thick can not be inserted, too thin can not effectively collect bile.

In the embodiment of the utility model, the first catheter 1 is 5mm long and is inserted into the depth of the common bile duct according to the physiological structure of the rat and the stress response of the rat; the inner diameter of the first conduit 1 is 0.31mm. The first catheter 1 is inserted into the common bile duct of the rat and fixed by means of a mononodule 2. Specifically, after the first catheter 1 is inserted into the common bile duct, the unijunction 2 is positioned outside and closely attached to the common bile duct, the common bile duct and the unijunction 2 are bound together and fixed through a suture line, and meanwhile, the unijunction 2 can also block the opening at the cannula of the common bile duct. The third catheter 5 is 8mm long and is inserted into the depth of the duodenum; the inner diameter of the third conduit 5 is 0.61mm. The third catheter 5 is inserted into the rat's duodenum and secured by the first double nodule 4. Specifically, after the third catheter 5 is inserted into the duodenum, the duodenum is wrapped by a suture line, so that the node, which is close to the third catheter 5, in the first double nodes 4 is in the duodenum and is prevented from falling off, and the node, which is far away from the third catheter 5, in the first double nodes 4 is out of the duodenum and blocks the opening at the duodenum cannula. Further, a plurality of small holes are arranged on the third conduit 5, so that food residues can flow out better, and blockage is avoided.

In the embodiment of the utility model, the second catheter 3 is exposed from the back of the rat, and is partially positioned outside the rat, and other parts are connected at the second catheter 3, so that bile can be periodically taken for a long time.

In the embodiment of the utility model, the rat bile duct catheter is made of polyurethane or silica gel, is safe and environment-friendly, has smooth surface and moderate hardness, is favorable for bile duct intubation, and is not easy to damage the bile duct intubation and reduce stress reaction.

According to the embodiment of the utility model, the first catheter is fixed on the side of the common bile duct through the single nodule after being inserted into the common bile duct, the third catheter is fixed on the side of the duodenum through the first double nodule after being inserted into the duodenum, the second catheter is positioned outside the rat body, bile can be taken out from the second catheter, the physiological structure of the rat is met, the fixed catheter is not easy to fall off, the requirement of periodically collecting bile for a long time is met, and the requirement of animal welfare is met.

Example two

In the embodiment of the present utility model, the same reference numerals as those of the first embodiment are used for the same components as those of the first embodiment, including all the features described in the first embodiment, and will not be described again.

As shown in fig. 1, the second catheter 3 includes a first sub-catheter 31, a second double-nodule 32, a second sub-catheter 33, a third double-nodule 34, and a third sub-catheter 35. The first sub-catheter 31, the second double nodule 32, the second sub-catheter 33, the third double nodule 34 and the third sub-catheter 35 are connected in sequence, through which bile may flow.

The operation process of catheterizing the bile duct of the rat body by catheterizing the bile duct of the rat body comprises the following steps: surgical windowing is performed on the abdomen and the back of the rat, the first catheter 1 is inserted into the common bile duct of the rat from the abdomen, and the single nodule 2 is fixed outside the common bile duct; threading a first sub-catheter 31 from the abdomen to the back, the second double nodule 32 being secured to the back muscle; the second sub-catheter 33 is left on the back; a third sub-catheter 35 is passed from the back to the abdomen, from which the third catheter 5 is inserted into the duodenum of the rat and the first double nodule 4 is fixed to the duodenum; the third double nodule 34 is brought into position and anchored to the back muscle. The second sub-catheter 33 is placed on the back and is positioned outside the rat body, and bile can be periodically taken from the second sub-catheter 33 for a long time after an operation when the experimental animal is in a waking state.

Specifically, the second double nodule 32 is secured to the back muscle by tying the back muscle and the second double nodule 32 together with a suture that is wrapped around and secured to the middle of the second double nodule 32. One of the second double nodules 32 after fixation is located outside the rat body and one of the nodules is located inside the rat body. The method of securing the third double nodule 34 to the back muscle is the same and will not be described in detail herein. Thus, the rat bile duct portions can be better fixed in the rat body, and only the second sub-duct 33 is located outside the rat body, reducing the influence on the daily activities of the rat.

Preferably, the inner pipe diameter of the first sub-catheter 31 is smaller than the inner pipe diameter of the second sub-catheter 33, the inner pipe diameter of the first sub-catheter 31 is equivalent to the inner pipe diameter of the first catheter 1, and when bile flows out of the first catheter 1, the first sub-catheter 31 and the second sub-catheter 33, the fluid pressure in the second sub-catheter 33 can be reduced, so that bile collection can be easily controlled.

According to the embodiment of the utility model, the first catheter is fixed on the side of the common bile duct through the single nodule after being inserted into the common bile duct, the third catheter is fixed on the side of the duodenum through the first double nodule after being inserted into the duodenum, the second catheter is positioned outside the rat body, bile can be taken out from the second catheter, the physiological structure of the rat is met, the fixed catheter is not easy to fall off, the requirement of periodically collecting bile for a long time is met, and the requirement of animal welfare is met.

Example III

In the embodiment of the present utility model, the same reference numerals as those of the first embodiment or the second embodiment are used for the same components as those of the first embodiment or the second embodiment, and all features described in the first embodiment or the second embodiment are included, which will not be described herein.

Fig. 2 is a schematic diagram of an experimental apparatus provided in a third embodiment of the present utility model. As shown in fig. 2, the experimental apparatus includes the rat bile duct catheter, the implanter, and the syringe described in the first embodiment or the second embodiment. The implantable device is used as a relay between the rat bile duct and the outside, and is provided with interfaces with other components, such as a syringe. The injector is connected with the second catheter 3 through the implantation device, and bile is taken out from the second catheter 3, or the injector is connected with the second catheter 3 through the implantation device, and artificial bile, liquid medicine, nutrients and the like are supplied to the duodenum.

According to the embodiment of the utility model, the first catheter is fixed on the side of the common bile duct through the single nodule after being inserted into the common bile duct, the third catheter is fixed on the side of the duodenum through the first double nodule after being inserted into the duodenum, the second catheter is positioned outside the rat body, bile can be taken out from the second catheter, the physiological structure of the rat is met, the fixed catheter is not easy to fall off, the requirement of periodically collecting bile for a long time is met, and the requirement of animal welfare is met.

Example IV

In the embodiment of the present utility model, the same reference numerals as those of the first embodiment or the second embodiment are used for the same components as those of the first embodiment or the second embodiment, and all features described in the first embodiment or the second embodiment are included, which will not be described herein.

Fig. 3 is a schematic diagram of an experimental apparatus according to a fourth embodiment of the present utility model. As shown in fig. 3, the experimental apparatus includes the rat bile duct catheter, the implanter, and the bile circulator described in the first embodiment or the second embodiment. The implantation device is used as a relay between the rat bile duct and the outside, and is provided with interfaces with other components, such as a bile circulator. The bile circulator is connected with the second catheter 3 through the implantation device, and forms a closed loop of bile flow passage.

According to the embodiment of the utility model, the first catheter is fixed on the side of the common bile duct through the single nodule after being inserted into the common bile duct, the third catheter is fixed on the side of the duodenum through the first double nodule after being inserted into the duodenum, the second catheter is positioned outside the rat body, bile can be taken out from the second catheter, the physiological structure of the rat is met, the fixed catheter is not easy to fall off, the requirement of periodically collecting bile for a long time is met, and the requirement of animal welfare is met. When bile is not needed to be taken, a closed loop bile flow channel is formed from the common bile duct to the duodenum, so that normal physiological functions of rats are maintained, and daily activities of the rats are not influenced.

While embodiments of the present utility model have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the utility model, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the utility model.

Claims (10)

1. A rat bile duct catheter, comprising:

a first conduit (1);

a unijunction (2) connected to the first conduit (1);

a second conduit (3) connected to the unijunction (2);

a first double nodule (4) connected to the second catheter (3); and

a third conduit (5) connected to the first double nodule (4);

the first catheter (1) is inserted into a common bile duct and fixed through the unijunction (2), the third catheter (5) is inserted into a duodenum and fixed through the first double-nodule (4), and bile is taken from the second catheter (3).

2. A rat bile duct according to claim 1, characterized in that the first duct (1) is 5mm long.

3. A rat bile duct according to claim 1, characterized in that the inner diameter of the first duct (1) is 0.31mm.

4. A rat bile duct according to claim 1, characterized in that the third duct (5) is 8mm long.

5. A rat bile duct according to claim 1, characterized in that the inner diameter of the third duct (5) is 0.61mm.

6. A rat bile duct according to claim 1, characterized in that a plurality of small holes are provided in the third duct (5).

7. A rat bile duct according to claim 1, characterized in that the second duct (3) comprises a first sub-duct (31), a second double-nodule (32), a second sub-duct (33), a third double-nodule (34) and a third sub-duct (35);

the second double nodule (32) and the third double nodule (34) are fixed to the back muscle, the second sub-catheter (33) is located outside the rat body, and bile is taken at the second sub-catheter (33).

8. A rat bile duct according to claim 7, characterized in that the inner tube diameter of the first sub-duct (31) is smaller than the inner tube diameter of the second sub-duct (33).

9. An experimental set-up comprising a rat bile duct catheter, an implanter and a syringe according to any of claims 1-8;

the injector is connected with the second catheter (3) through the implantation device, and bile is taken out from the second catheter (3).

10. An experimental set-up comprising a rat bile duct catheter, an implanter and a bile circulator as claimed in any of claims 1-8;

the bile circulator is connected with the second catheter (3) through the implantation device to form a closed loop bile flow channel.