CN115120841A

CN115120841A - Rat bile duct catheter

Info

Publication number: CN115120841A
Application number: CN202210777979.8A
Authority: CN
Inventors: 黄川�; 郭俊秀
Original assignee: Rwd Life Science Co ltd
Current assignee: Rwd Life Science Co ltd
Priority date: 2022-07-04
Filing date: 2022-07-04
Publication date: 2022-09-30

Abstract

The embodiment of the invention provides a rat biliary duct, which comprises: a first conduit (1); a single node (2) connected to the first conduit (1); a second conduit (3) connected to the single junction (2); a first double node (4) connected to the second conduit (3); a third duct (5) connected to the first double node (4); the first catheter (1) is inserted into the common bile duct and fixed by a single node (2), the third catheter (5) is inserted into the duodenum and fixed by a first double node (4), and bile is taken at the second catheter (3). According to the embodiment of the invention, the first catheter is inserted into the common bile duct and then fixed on the common bile duct side through the single node, the third catheter is inserted into the duodenum and then fixed on the duodenum side through the first double node, the second catheter is positioned outside the rat body, bile can be taken from the second catheter, the physiological structure of the rat is met, the bile does not fall off easily after being fixed, the requirement of periodically collecting the bile for a long time is met, and the requirement of animal welfare is met.

Description

Rat bile duct catheter

Technical Field

The invention relates to the technical field of animal experiments, in particular to a rat bile duct conduit and an experimental device with the same.

Background

In the process of drug development, it is often necessary to periodically collect bile from an experimental animal to measure the half-life of a drug to determine the efficacy of the drug, to perform toxicological analysis of the drug, and the like.

At present, most of methods for collecting bile of experimental animals are to anaesthetize the experimental animals and then extract the bile in an operation. The method has great harm to experimental animals, the experimental animals cannot survive for a long time, long-time periodic collection is difficult to achieve, and the method does not meet the requirement of animal welfare.

If long-time periodic bile collection needs to be completed outside the experimental animal body, and the common bile duct and the duodenum of the experimental animal need to be communicated when the bile collection is not performed, a bile flow channel is constructed, and bile duct intubation is indispensable operation on the experimental animal. However, the existing bile duct conduit is simple in design and easy to fall off, and the requirement of long-time periodic collection is difficult to meet.

Disclosure of Invention

The embodiment of the invention provides a rat bile duct, aiming at solving the problem that the bile duct is easy to fall off when experimental animals periodically collect bile for a long time in the prior art.

In a first aspect, there is provided a rat biliary duct comprising:

a first conduit (1);

a single node (2) connected to the first conduit (1);

a second conduit (3) connected to the single junction (2);

a first double node (4) connected to the second conduit (3); and

a third duct (5) connected to the first double node (4);

wherein, a first catheter (1) is inserted into the common bile duct and fixed by a single node (2), a third catheter (5) is inserted into the duodenum and fixed by a first double node (4), and bile is taken from a second catheter (3).

In a second aspect, there is provided a test device comprising a rat biliary duct, an implant and a syringe as described above;

the injector is connected with the second conduit (3) through the implant device, and bile is taken from the second conduit (3).

In a third aspect, there is provided an experimental apparatus comprising a rat bile duct conduit, an implanter and a bile circulator as described above;

the bile circulator is connected with a second conduit (3) through the implant to form a closed loop bile flow channel.

According to the embodiment of the invention, the first catheter is inserted into the common bile duct and then fixed on the common bile duct side through the single node, the third catheter is inserted into the duodenum and then fixed on the duodenum side through the first double node, the second catheter is positioned outside the rat body, bile can be taken from the second catheter, the physiological structure of the rat is met, the bile does not fall off easily after being fixed, the requirement of periodically collecting the bile for a long time is met, and the requirement of animal welfare is met.

Drawings

The foregoing and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:

FIG. 1 is a schematic diagram of a rat bile duct provided by an embodiment of the invention;

FIG. 2 is a schematic diagram of an experimental apparatus provided in the third embodiment of the present invention;

fig. 3 is a schematic diagram of an experimental apparatus provided in the fourth embodiment of the present invention.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar modules or modules having the same or similar functionality throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention. On the contrary, the embodiments of the invention include all changes, modifications and equivalents coming within the spirit and terms of the claims appended hereto.

The method for extracting the bile from the experimental animal in the operation after the experimental animal is anesthetized needs to perform the operation windowing on the abdomen of the experimental animal, suture the wound after the bile is extracted, and realize the primary bile collection. The method has great harm to experimental animals, and the experimental animals can not bear the harm for many times and die, so that the drug effect of the drug in different stages in the same experimental animal can not be measured.

The bile duct intubation is carried out on the experimental animal, and the bile is periodically collected in vitro of the experimental animal, so that the bile can be collected in vitro of the experimental animal for multiple times when the experimental animal is in a sober state only by embedding the bile duct in the experimental animal through one operation; when the bile is not collected, the common bile duct and the duodenum of the experimental animal are still communicated to form a closed loop bile flow channel, so that the normal physiological function of the experimental animal is maintained. The method requires that the influence of the bile duct conduit on the experimental animal is small, the stress response of the experimental animal is reduced as much as possible, and the bile duct conduit can still be fixed on the common bile duct and the duodenum and is not easy to fall off under the condition that the experimental animal carries the bile duct conduit for a long time to move.

Example one

Fig. 1 is a schematic view of a rat biliary duct provided in an embodiment of the present invention. As shown in fig. 1, the rat biliary duct includes a first duct 1, a single node 2, a second duct 3, a first double node 4, and a third duct 5. The first conduit 1, the single node 2, the second conduit 3, the first double node 4 and the third conduit 5 are connected in sequence, and bile can flow therethrough.

During the feeding process of the rat, food enters duodenum from stomach, and bile flows into duodenum from common bile duct to promote digestion of fat in food. Collecting bile and forming a closed loop bile flow channel requires connecting the rat bile duct conduit between the common bile duct and the duodenum. Thus, the design of the biliary tract in rats is based largely on a thorough understanding of the physiological structure of the rats and the stress response of the rats.

The depth of the rat bile duct catheter inserted into the common bile duct needs to be proper, bile duct obstruction can be caused if the depth is too long, and the bile duct catheter can fall off easily if the depth is too short; the depth of inserting into duodenum also needs to be appropriate, and overlength can cause ileus, and the short then easily drops, and intestinal tissue is smooth soft moreover, has further increased the fixed degree of difficulty, and fixed not firm easily leads to the experiment failure. The thickness of each part of the rat biliary duct needs to be proper, and the thick part can not be inserted, and the thin part can not effectively collect bile.

In the present example, the first catheter 1 was 5mm long to be inserted into the common bile duct to a depth according to the physiological configuration of the rat and the stress response of the rat; the inner tube diameter of the first conduit 1 was 0.31 mm. The first catheter 1 is inserted into the common bile duct of the rat and fixed by a single node 2. Specifically, after the first catheter 1 is inserted into the common bile duct, the single knot 2 is positioned outside the common bile duct and is tightly attached to the common bile duct, the common bile duct and the single knot 2 are tied together and fixed through a suture, and meanwhile, the single knot 2 can also block an opening at the intubation position of the common bile duct. The third catheter 5 is 8mm long and is inserted into the duodenum to a depth; the inner tube diameter of the third conduit 5 was 0.61 mm. A third catheter 5 is inserted into the duodenum of the rat and fixed by a first double node 4. Specifically, after the third catheter 5 is inserted into the duodenum, the duodenum is packed by suture, so that the nodes close to the third catheter 5 in the first double nodes 4 are in the duodenum and are prevented from falling off, and the nodes far away from the third catheter 5 in the first double nodes 4 are outside the duodenum and block the opening at the duodenal intubation. Further, a plurality of small holes are provided in the third conduit 5 to allow better outflow of the food waste, avoiding clogging.

In the embodiment of the invention, the second conduit 3 is exposed from the back of the rat, part of the second conduit is positioned outside the rat, and other parts are connected at the second conduit 3, so that bile can be taken periodically for a long time.

In the embodiment of the invention, the rat bile duct conduit is made of polyurethane or silica gel and the like, is safe and environment-friendly, has smooth surface and moderate hardness, is beneficial to bile duct intubation, is not easy to damage and reduces stress reaction.

Example two

In the embodiment of the present invention, the same components as those in the first embodiment are denoted by the same reference numerals as those in the first embodiment, and all the features described in the first embodiment are included, and are not described again.

As shown in fig. 1, the second catheter 3 includes a first sub-catheter 31, a second double node 32, a second sub-catheter 33, a third double node 34, and a third sub-catheter 35. The first sub-duct 31, the second double node 32, the second sub-duct 33, the third double node 34 and the third sub-duct 35 are connected in sequence, and bile can flow therethrough.

The operation process of bile duct intubation on the rat and bile duct conduit implantation in the rat comprises the following steps: performing surgical fenestration on the abdomen and back of the rat, inserting the first catheter 1 into the common bile duct of the rat from the abdomen, and fixing the single node 2 outside the common bile duct; passing the first sub-catheter 31 from the abdomen to the back with the second double node 32 secured to the back muscle; the second sub-catheter 33 is left on the back; the third sub-catheter 35 is passed from the back to the abdomen, the third catheter 5 is inserted into the duodenum of the rat from the abdomen, and the first double node 4 is fixed to the duodenum; the third double tubercle 34 is adjusted to the appropriate position and secured to the back muscles. The second sub-catheter 33 is left on the back and is positioned outside the rat body, and bile can be periodically taken out at the second sub-catheter 33 for a long time after the operation when the experimental animal is in a waking state.

Specifically, the second double node 32 is secured to the dorsal muscle by tying the dorsal muscle and the second double node 32 together with a suture that is secured in a winding manner around the middle of the second double node 32. One of the second double nodules 32 was located outside the rat and one nodule was located inside the rat after fixation. The method of securing third double tubercle 34 to the back muscles is the same and will not be described further. Therefore, a plurality of parts of the bile duct conduit of the rat can be better fixed in the body of the rat, and only the second sub-conduit 33 is positioned outside the body of the rat, so that the influence on the daily activities of the rat is reduced.

Preferably, the inner diameter of the first sub-conduit 31 is smaller than the inner diameter of the second sub-conduit 33, the inner diameter of the first sub-conduit 31 is equivalent to the inner diameter of the first conduit 1, and when bile flows out of the first conduit 1, the first sub-conduit 31 and the second sub-conduit 33, the fluid pressure in the second sub-conduit 33 can be reduced, and bile collection can be easily controlled.

EXAMPLE III

In the embodiment of the present invention, the same components as those in the first embodiment or the second embodiment use the same reference numerals as those in the first embodiment or the second embodiment, and include all the features described in the first embodiment or the second embodiment, which are not described herein again.

Fig. 2 is a schematic diagram of an experimental apparatus provided in the third embodiment of the present invention. As shown in fig. 2, the experimental set-up comprised the rat bile duct catheter, the implant and the syringe described in example one or example two. The implant is used as a transfer between a rat bile duct conduit and the outside, and is provided with an interface with other components, such as a syringe. The injector is connected to the second conduit 3 through the implant device to take bile from the second conduit 3, or the injector is connected to the second conduit 3 through the implant device to supply artificial bile, medicinal liquid, nutrients, etc. to the duodenum.

Example four

In the embodiment of the present invention, the same components as those in the first embodiment or the second embodiment are denoted by the same reference numerals as those in the first embodiment or the second embodiment, and all the features described in the first embodiment or the second embodiment are included, and are not described again here.

Fig. 3 is a schematic diagram of an experimental apparatus provided in the fourth embodiment of the present invention. As shown in fig. 3, the experimental set-up comprised the rat bile duct conduit, the implant and the bile circulator described in example one or example two. The implant is used as a transfer between a rat bile duct conduit and the outside, and is provided with an interface with other components, such as a bile circulator. The bile circulator is connected with the second conduit 3 through the implant to form a closed loop bile flow channel.

According to the embodiment of the invention, the first catheter is inserted into the common bile duct and then fixed on the common bile duct side through the single node, the third catheter is inserted into the duodenum and then fixed on the duodenum side through the first double node, the second catheter is positioned outside the rat body, bile can be taken from the second catheter, the physiological structure of the rat is met, the bile does not fall off easily after being fixed, the requirement of periodically collecting the bile for a long time is met, and the requirement of animal welfare is met. When the bile does not need to be taken, a closed loop bile flow channel is formed from the common bile duct to the duodenum, so that the normal physiological function of the rat is maintained, and the daily activity of the rat is not influenced.

Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.

Claims

1. A rat biliary duct comprising:

a first conduit (1);

a single nodule (2) connected to the first conduit (1);

a second catheter (3) connected to the single node (2);

a first double node (4) connected to the second conduit (3); and

a third conduit (5) connected to the first double node (4);

wherein the first catheter (1) is inserted into the common bile duct and fixated by the single node (2), the third catheter (5) is inserted into the duodenum and fixated by the first double node (4), and bile is taken at the second catheter (3).

2. The rat bile duct according to claim 1, characterized in that the first duct (1) is 5mm long.

3. The rat biliary duct according to claim 1, wherein the first duct (1) has an internal duct diameter of 0.31 mm.

4. The rat bile duct according to claim 1, characterized in that the third duct (5) is 8mm long.

5. The rat biliary duct according to claim 1, wherein the third duct (5) has an internal duct diameter of 0.61 mm.

6. The rat biliary duct according to claim 1, wherein a plurality of small holes is provided on the third duct (5).

7. The rat biliary duct according to claim 1, wherein the second duct (3) comprises a first sub-duct (31), a second double node (32), a second sub-duct (33), a third double node (34), and a third sub-duct (35);

the second double node (32) and the third double node (34) are fixed on the back muscle, the second sub-conduit (33) is positioned outside the rat body, and bile is taken at the second sub-conduit (33).

8. The rat biliary duct according to claim 7, wherein an inner diameter of the first sub-duct (31) is smaller than an inner diameter of the second sub-duct (33).

9. A laboratory device comprising a rat biliary duct according to any one of claims 1 to 8, an implant, and a syringe;

10. An experimental set-up comprising a rat bile duct conduit according to any of claims 1-8, an implant and a bile circulator;

the bile circulator is connected with the second conduit (3) through the implant device to form a closed loop bile flow channel.