CN219399034U

CN219399034U - Solid-phase extraction 96-hole plate positive pressure instrument auxiliary device

Info

Publication number: CN219399034U
Application number: CN202222761147.5U
Authority: CN
Inventors: 李明珠; 吴颖; 吴萍萍; 于嘉屏; 龚晓雪; 张雪珂; 刘洋
Original assignee: SHANGHAI DIAN MEDICAL INSPECTION CO LTD
Current assignee: SHANGHAI DIAN MEDICAL INSPECTION CO LTD
Priority date: 2022-10-20
Filing date: 2022-10-20
Publication date: 2023-07-25
Anticipated expiration: 2032-10-20

Abstract

The utility model discloses an auxiliary device of a solid-phase extraction 96-pore plate positive pressure instrument, which relates to the technical field of solid-phase extraction equipment and comprises a solid-phase extraction pore plate body, wherein the bottom end of the solid-phase extraction pore plate body is provided with a gun head, and the bottom end of the gun head is provided with an injector; the tip part of the gun head is connected with the outlet end of the injector, and the other end of the gun head is connected with the liquid outflow end of the solid phase extraction pore plate body; the syringe had a capacity of 25mL and the gun head had a capacity of 200. Mu.L. In the utility model, the injector is a common laboratory equipment, the injector is cheap and easy to obtain, the gun head is a common laboratory consumable, the whole set of brand new device can be assembled at any time, the device is contacted with the 96 Kong Guxiang extraction column blocked by the sample, and the negative pressure extraction is carried out without forming sample cross contamination, so that the problem of difficulty in the pretreatment process of the positive pressure solid phase extraction of the 96-well plate by clinical detection personnel can be solved, the operation efficiency of the pretreatment process is improved, and the sensitivity and the accuracy of detection are improved.

Description

Solid-phase extraction 96-hole plate positive pressure instrument auxiliary device

Technical Field

The utility model relates to the technical field of solid phase extraction equipment, in particular to an auxiliary device of a solid phase extraction 96-hole plate positive pressure instrument.

Background

The solid phase extraction is to make the liquid sample pass through the stationary phase adsorbent by utilizing the liquid chromatography separation principle of selective adsorption and selective elution, to keep a certain component in the sample by utilizing the adsorbent, to wash out the impurity on the adsorbent by selecting a proper solvent, to quickly elute the adsorbent by using a small amount of solvent to obtain the required component, and to achieve the purposes of quick separation, purification and concentration by utilizing the solid phase extraction technology, the extraction process comprises the steps of pre-leaching, sample loading, washing and elution, and a 96-hole positive pressure device is generally used for applying a certain pressure above the extraction column by using compressed air or nitrogen to control the activation, leaching and elution speeds of the extraction column, and simultaneously, the solution is easier to enter the stationary phase to improve the extraction efficiency.

The existing positive pressure type solid phase extraction instrument is mature in technology, but still has certain defects, in the solid phase extraction process of a positive pressure 96-well plate, a positive pressure device sometimes leads a sample to not pass through an SPE column smoothly in clinical detection, experimental operation efficiency is reduced, detection sensitivity and accuracy are affected, and clinical detection staff has certain difficulty in the pretreatment process of the positive pressure solid phase extraction of the 96-well plate.

Disclosure of Invention

The utility model aims to solve the defects that the existing positive pressure type solid phase extraction instrument is mature in technology, but still has certain defects, in the solid phase extraction process of a positive pressure 96-well plate, a positive pressure device sometimes leads a viscous blood sample in clinical detection to not pass through an SPE (solid phase extraction) column smoothly, the experimental operation efficiency is reduced, the sensitivity and the accuracy of detection are affected, and clinical detection staff has certain difficulty in the positive pressure solid phase extraction pretreatment process of the 96-well plate.

In order to achieve the above purpose, the present utility model adopts the following technical scheme:

the utility model provides a solid phase extraction 96 orifice plate malleation appearance auxiliary device, includes the solid phase extraction orifice plate body, the bottom of solid phase extraction orifice plate body is provided with the rifle head, and the bottom of rifle head is provided with the syringe, the tip of rifle head is connected with the outlet terminal of syringe, and the other end of rifle head is connected with the liquid outflow end of solid phase extraction orifice plate body.

As a further description of the above technical solution:

the syringe had a capacity of 25mL and the gun head had a capacity of 200. Mu.L.

In summary, due to the adoption of the technical scheme, the beneficial effects of the utility model are as follows:

in the utility model, the injector is a common laboratory equipment, the injector is cheap and easy to obtain, the gun head is a common laboratory consumable, the whole set of brand new device can be assembled at any time, the device is contacted with the 96 Kong Guxiang extraction column blocked by the sample, and the negative pressure extraction is carried out without forming sample cross contamination, so that the problem of difficulty in the pretreatment process of the positive pressure solid phase extraction of the 96-well plate by clinical detection personnel can be solved, the operation efficiency of the pretreatment process is improved, and the sensitivity and the accuracy of detection are improved.

Drawings

FIG. 1 is a schematic diagram of an auxiliary device of a solid phase extraction 96-well plate positive pressure instrument in the utility model;

FIG. 2 is a schematic diagram of the connection structure between the gun head and the injector according to the present utility model;

FIG. 3 is a schematic top view of a solid phase extraction 96-well plate according to the present utility model.

Legend description:

1. solid phase extraction of 96-well plate body; 2. a gun head; 3. a syringe.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Referring to fig. 1-3, an auxiliary device of a solid-phase extraction 96-well plate positive pressure instrument comprises a solid-phase extraction 96-well plate body 1, a gun head 2 is arranged at the bottom end of the solid-phase extraction 96-well plate body 1, an injector 3 is arranged at the bottom end of the gun head 2, the tip end of the gun head 2 is connected with the outlet end of the injector 3, the other end of the gun head 2 is connected with the liquid outlet end of the solid-phase extraction 96-well plate body 1, when the inside of the solid-phase extraction 96-well plate body 1 is blocked by a sample, the gun head 2, the injector 3 and the solid-phase extraction 96-well plate body 1 can be connected through the gun head 2 and the injector 3, a negative pressure system can be formed in the inside of the gun head 2, the injector 3 and the solid-phase extraction 96-well plate body 1, the hole site blocked by a viscous blood sample can smoothly pass through a solid-phase extraction column, the gun head 2 needs to be cut into half, and half of the gun head needs to be tightly connected with the injector 3 and the solid-phase extraction 96-well plate body 1.

Further, the capacity of the injector 3 is 25mL, the capacity of the gun head 2 is 200 mu L, and the manual air suction is performed by using the 25mL injector 3, so that the whole device can form a negative pressure system due to the airtight connection of the device, and no gas can enter other parts at the position of the hole of the solid phase extraction 96-well plate.

Working principle: when the solid phase extraction instrument is used, the steps of activation, sample loading, leaching, elution and the like are carried out on the solid phase extraction instrument, if individual hole sites are blocked by samples in the positive pressure to dry process after sample loading, the solid phase extraction 96-well plate body 1 is tightly connected with the 25mL syringe 3 through one half 200 mu L of the gun head 2, the 25mL syringe 3 is used for carrying out negative pressure extraction for a plurality of times, one half 200 mu L of the gun head 2 is replaced each time until the samples pass through the 96 Kong Guxiang extraction plate body 1, and then the steps of leaching, elution and the like are sequentially carried out.

The foregoing is only a preferred embodiment of the present utility model, but the scope of the present utility model is not limited thereto, and any person skilled in the art, who is within the scope of the present utility model, should make equivalent substitutions or modifications according to the technical scheme of the present utility model and the inventive concept thereof, and should be covered by the scope of the present utility model.

Claims

1. The utility model provides a solid phase extraction 96 orifice plate malleation appearance auxiliary device, includes solid phase extraction 96 orifice plate body (1), its characterized in that: the solid phase extraction 96-well plate comprises a solid phase extraction 96-well plate body (1), and is characterized in that a gun head (2) is arranged at the bottom end of the solid phase extraction 96-well plate body (1), an injector (3) is arranged at the bottom end of the gun head (2), the tip end of the gun head (2) is connected with an outlet end of the injector (3), and the other end of the gun head (2) is connected with a liquid outflow end of the solid phase extraction 96-well plate body (1).

2. The solid phase extraction 96-well plate positive pressure instrument auxiliary device according to claim 1, wherein: the volume of the syringe (3) is 25mL, and the volume of the gun head (2) is 200 mu L.