CN219342177U - Weightlessness simulation effect slewing device system capable of monitoring cell growth state on line in real time - Google Patents

Abstract

The utility model's weightless simulation effect rotary device system capable of real-time on-line monitoring of cell growth status includes: the rotary device body; Impedance changes; the peripheral detection and analysis module used to realize the detection, processing and analysis of cell impedance signals is connected to the impedance sensitive electrodes of the rotary device body and the cell culture container respectively. The utility model can monitor the growth state of cells in real time, without a mark and with high sensitivity, and provides monitoring technical support for the objective and accurate research of ground simulated weightlessness cell culture experiments; at the same time, the utility model can also be used in the fields of biotechnology, medical diagnosis, etc. Real-time cell growth monitoring in rotor-based cell culture industry and experimental research.

Description

A weightless simulation effect rotary device system that can monitor cell growth status online in real time

technical field

The utility model relates to a biological monitoring device, in particular to a weightless simulating effect rotary device system capable of monitoring cell growth status on-line in real time.

Background technique

The gyratory device for cell weightlessness simulation effect is an important basic platform for cell culture research in a weightless environment on the ground. However, none of the existing gyratory devices for weightlessness simulation effect has the function of real-time online monitoring of cell growth status, and cannot capture the growth of cells during the gyration process. Cells change, so important hidden information can be lost. Conventional cell proliferation detection methods, such as microscope imaging, fluorescent labeling detection, flow cytometry, etc., are either difficult to achieve quantitative detection, or require special treatment of cells, or need to remove cells to terminate culture, and cannot realize the cells in the process of rotation. Growth real-time monitoring.

Utility model content

The purpose of the utility model is to provide a weightless simulation effect rotary device system which can monitor cell growth status online in real time.

The utility model's weightless simulation effect rotary device system capable of real-time online monitoring of cell growth status includes: a rotary device body for realizing continuous cell rotation with controllable conditions; a cell culture container with impedance sensitive electrodes for cell growth Adhesive growth provides carriers and senses impedance changes during cell culture. The cell culture container with impedance-sensitive electrodes is set in the body of the rotary device; peripheral detection and analysis for the detection, processing and analysis of cell impedance signals The module is connected to the body of the rotary device and the impedance-sensitive electrodes of the cell culture container respectively.

The weightless simulation effect rotary device system of the utility model that can monitor cell growth status online in real time, wherein, the cell culture container includes a fully-sealed cell culture pool for containing cells, and several impedance sensitive cells are arranged at the bottom of the cell culture pool. The electrodes, the impedance-sensitive electrodes are connected to the pin pads, and the pin pads are connected to the impedance reading circuit of the peripheral detection and analysis module.

The weightless simulation effect rotary device system of the utility model capable of real-time on-line monitoring of cell growth state, wherein, the shape of the impedance-sensitive electrode is interdigitated, ring-shaped or circular.

The utility model can real-time on-line monitoring cell growth status of the weightless simulation effect rotary device system, wherein the impedance sensitive electrode is directly processed on the inner wall of the bottom surface of the cell culture pool or embedded in the bottom surface of the culture container after being prepared on the glass substrate.

The weightless simulation effect rotary device system of the present utility model can monitor the cell growth state on-line in real time, wherein the impedance sensitive electrode is connected to the pin pad, and the pin pad leads out a wire by welding or clamping, and the wire is connected to the body of the rotary culture device To the impedance reading circuit of the peripheral detection and analysis module.

The weightlessness simulation effect rotary device system of the utility model that can monitor the cell growth state online in real time, wherein, the rotary device body includes a support end, a rotary transmission structure, a rotating shaft connecting plate, and a rotary condition control module, and the support end is separated from the There are support holes on both sides of the rotating shaft connecting plate, the rotating shaft connecting plate is rotatably installed on the supporting end through the supporting hole of the supporting end, and the rotation condition control module includes a motor installed on the supporting end and a control Assemblies, the output shaft of the motor is connected to the rotating shaft connecting plate, the cell culture container is installed on the rotating shaft connecting plate, so that the cell culture container and the rotating shaft connecting plate can rotate at the same speed, and the control component of the rotation condition control module is used to connect the rotating shaft The rotation parameters of the plate are set and adjusted, and the rotary transmission structure includes an electrical connector rotatably installed on the support end through the support hole, and the front-end leads of the electrical connector are connected to the pin pads of the cell culture container, The back-end leads of the electrical connector are connected with the peripheral detection and analysis module.

The utility model can real-time on-line monitor cell growth state weightless simulation effect rotary device system, wherein, the peripheral detection and analysis module includes impedance detection equipment and a host computer, the impedance detection equipment is connected with the host computer, and the impedance detection equipment is connected with the electrical connector The connection is used to collect changes in cell impedance, and the host computer is used to process the data of the impedance detection equipment and output the analysis results on the screen of the host computer.

The weightless simulation effect rotary device system of the utility model that can monitor the cell growth state on-line in real time, wherein the peripheral detection and analysis module also includes a time-sharing sequential gating module, which is connected to the electrical connector and impedance detection Between devices, it is used for time-sharing sequential gating to detect the current or voltage of one or more impedance-sensitive electrodes.

The weightless simulation effect rotary device system of the present utility model can monitor the cell growth state on-line in real time, wherein the impedance detection equipment is a commercial electrical impedance meter, an electrochemical workstation or an impedance acquisition and analysis module built independently, which is used to detect each cell in the same round. The current and voltage values detected by each electrode are calculated and processed, and the impedance-time curve corresponding to the average state of the cells or the state of the local area is output according to the requirements.

The weightless simulation effect rotary device system of the utility model can monitor the growth state of cells in real time online, wherein the impedance detection equipment is an impedance acquisition and analysis module built independently, and the impedance acquisition and analysis module built independently includes a data acquisition chip, a digital lock-in amplifier, The display screen and the data acquisition chip are connected with the time-sharing sequential gating module.

The weightless simulation effect rotary device system of the utility model, which can monitor the cell growth state on-line in real time, is oriented to the field of aerospace medical experiments, and can monitor the cell growth state in real time, without a mark, and with high sensitivity, and is an objective and accurate study of the ground simulated weightlessness cell culture experiment. Provide monitoring technical support. At the same time, the weightless simulating effect rotary device system of the present invention, which can monitor cell growth status online in real time, can also be used for real-time cell growth monitoring in the gyrator-based cell culture industry and experimental research in the fields of biotechnology, medical diagnosis and the like.

Description of drawings

Fig. 1 is the structural representation of the cell culture container of the weightless simulation effect rotary device system that can monitor the cell growth status on-line in real time of the present invention;

Fig. 2 is a structural schematic diagram of the rotary device body of the weightless simulation effect rotary device system capable of real-time on-line monitoring of the cell growth state of the present invention;

Fig. 3 is a structural schematic diagram of a weightless simulation effect rotary device system capable of real-time on-line monitoring of cell growth status of the present invention.

Detailed ways

As shown in Fig. 1, Fig. 2 and Fig. 3, the weightless simulation effect rotary device system of the present invention, which can monitor the cell growth state online in real time, includes: a cell culture container 1 with impedance-sensitive electrodes, which is used for cell attachment The growth provides a carrier and senses the impedance change in the process of cell culture; the body of the rotary device is used to realize the continuous rotation of cells with controllable conditions, and the cell culture container with impedance-sensitive electrodes is arranged in the body of the rotary device; it is used to realize The peripheral detection and analysis module for the detection, processing and analysis of cell impedance signals is respectively connected to the body of the rotary device and the impedance-sensitive electrodes of the cell culture container.

The weightless simulation effect rotary device system of the utility model that can monitor cell growth state online in real time, wherein, the cell culture container includes a fully enclosed cell culture pool for containing cells, and the bottom inner wall of the cell culture container 1 is arranged with several The same or different impedance-sensitive electrodes 11, the impedance-sensitive electrodes are connected to the pin pads 12, and the pin pads are connected to the impedance reading circuit of the peripheral detection and analysis module.

The cell culture container includes an airtight cell culture pool for containing cells. Several impedance sensitive electrodes are arranged at the bottom of the cell culture pool. Impedance reading circuit connection.

The cell culture container is fully airtight, so it can be used for cell culture in any posture (such as static flat, static tilt, continuous rotation).

The weightless simulation effect rotary device system of the utility model capable of real-time on-line monitoring of cell growth state, wherein, the shape of the impedance-sensitive electrode is interdigitated, ring-shaped or circular. The shape of the impedance-sensitive electrode is interdigitated, ring-shaped, circular or other special shapes whose detection sensitivity/resolution meets the test requirements.

The utility model can real-time on-line monitoring cell growth status of the weightless simulation effect rotary device system, wherein the impedance sensitive electrode is directly processed on the inner wall of the bottom surface of the cell culture pool or embedded in the bottom surface of the culture container after being prepared on the glass substrate.

The weightless simulation effect rotary device system of the present utility model can monitor the cell growth state on-line in real time, wherein the impedance sensitive electrode is connected to the pin pad, and the pin pad leads out a wire by welding or clamping, and the wire is connected to the body of the rotary culture device To the impedance reading circuit of the peripheral detection and analysis module.

The weightless simulation effect rotary device system of the present utility model can monitor cell growth status online in real time, wherein the rotary device body includes a support end 21, a rotary transmission structure, a rotating shaft connecting plate 23, and a rotary condition control module 24. The ends are separated from both sides of the rotating shaft connecting plate, and there are supporting holes on it. The rotating shaft connecting plate is rotatably installed on the supporting end through the supporting hole of the supporting end. The rotation condition control module includes a The motor and the control assembly, the output shaft of the motor is connected to the rotating shaft connecting plate, the cell culture container is installed on the rotating shaft connecting plate, so that the cell culture container and the rotating shaft connecting plate can rotate at the same speed, and the control assembly of the rotation condition control module is used for Set and adjust the rotation parameters of the connecting plate of the rotating shaft. The rotary transmission structure includes an electrical connector 25 rotatably installed on the support end through the support hole. The front-end leads of the electrical connector are connected to the pins of the cell culture container The pads are connected, and the back-end leads of the electrical connector are connected with the peripheral detection and analysis module.

The utility model can real-time on-line monitoring cell growth state weightless simulation effect rotary device system, wherein, the peripheral detection and analysis module includes impedance detection equipment 32 and host computer 33, the impedance detection equipment is connected with the host computer, and the impedance detection equipment is connected with the electrical The connector is used to collect changes in cell impedance, and the host computer is used to process the data of the impedance detection equipment and output the analysis results on the screen of the host computer.

The weightless simulation effect rotary device system of the utility model that can monitor the cell growth state on-line in real time, wherein the peripheral detection and analysis module also includes a time-sharing sequential gating module, which is connected to the electrical connector and impedance detection Between devices, it is used for time-sharing sequential gating to detect the current or voltage of one or more impedance-sensitive electrodes.

The weightless simulation effect rotary device system of the present utility model can monitor the cell growth state on-line in real time, wherein the impedance detection equipment is a commercial electrical impedance meter, an electrochemical workstation or an impedance acquisition and analysis module built independently, which is used to detect each cell in the same round. The current and voltage values detected by each electrode are calculated and processed, and the impedance-time curve corresponding to the average state of the cells or the state of the local area is output according to the requirements.

The weightless simulation effect rotary device system of the utility model can monitor the growth state of cells in real time online, wherein the impedance detection equipment is an impedance acquisition and analysis module built independently, and the impedance acquisition and analysis module built independently includes a data acquisition chip, a digital lock-in amplifier, The display screen and the data acquisition chip are connected with the time-sharing sequential gating module.

The weightless simulation effect rotary device system of the utility model, which can monitor the growth state of cells on-line in real time, can be applied to research fields such as medicine, life science and cell biology, and integrates a detection system based on electrical impedance spectroscopy, and has the ability to monitor cells on-line in real time. state function.

The weightless simulation effect rotary device system of the utility model capable of real-time on-line monitoring of cell growth status can realize on-line detection and continuous monitoring without marking, non-invasion, and not affecting normal growth of cells.

The utility model's weightless simulation effect rotary device system capable of real-time online monitoring of cell growth status mainly includes three parts: a cell culture container with impedance sensitive electrodes, a rotary device body and a peripheral detection and analysis module.

The cell culture container with impedance-sensitive electrodes provides a carrier for cell adherent growth and senses impedance changes during cell culture. The body of the rotary device realizes continuous rotation of cells under controllable conditions. The peripheral detection and analysis module realizes the detection of cell impedance signals, processing and analysis.

Several identical or different impedance-sensitive electrodes are arranged on the inner wall of the bottom of the cell culture container as required. The shape of the electrodes can be interdigitated, circular, circular or other optimized shapes. The electrodes can be directly processed on the inner wall of the bottom of the culture container or on glass, etc. The electrode is prepared on the easy-to-process substrate and embedded in the bottom of the culture container. The electrode has a lead pad, and the wire can be drawn from the lead pad by welding, clamping, etc., and connected to the peripheral detection and analysis module by rotating the culture device body. Impedance read circuit.

The slewing device body includes a support end, a slewing transmission structure, a rotating shaft connection plate, a slewing condition control module, and the like. A rotary transmission structure is installed in the support hole of the support end, which contains an electrical connector, which can realize reliable transmission of electrical signals in a rotary state. The connecting plate of the rotating shaft can be cantilevered or supported at both ends, and the cell culture container is installed on the connecting plate. The front-end leads of the electrical connector can be connected to lead wires on the pin pads of the cell culture container, or directly welded to the pin pads. The control circuit of the rotation condition control module can set and adjust parameters such as speed.

The peripheral detection and analysis module can be realized in two ways. The main components of one way include a commercial impedance analyzer, an electrochemical workstation or other impedance detection equipment and a host computer. Cell impedance changes are collected by the impedance analyzer, and the data is transmitted to the host computer. After the computer is processed by MATLAB, LabVIEW and other software, the analysis results are output on the upper computer screen. Another way is to form an integrated data detection, analysis, and display module through the construction of data analysis modules such as data acquisition chips, digital lock-in amplifiers, and display screens.

After adopting the above technical scheme, the cell weightlessness simulation effect rotary device system that can monitor the growth status online in real time has the following salient features:

Impedance detection electrodes are integrated in the airtight cell culture container, which can monitor the growth state of cells in the simulated weightless rotation state on the ground without marking, quantitatively, and in real time, breaking through the limitation that the growth status cannot be monitored in real time during the previous cell rotation culture process;

By placing multiple electrodes on the ground of the cell culture plate, and selecting the average value or partial focus according to the point of interest, it is possible to obtain both objectivity and accuracy when obtaining the growth of cells in the culture vessel;

By separately analyzing the changes in cell impedance on each electrode and monitoring the growth of cells in different regions, abnormal growth of cells during simulated weightlessness culture can be found in time, providing an important basis and reference for whether manual intervention is required.

The specific application examples of the weightless simulation effect rotary device system of the utility model that can monitor the growth state of cells in real time are as follows:

The cell culture container with impedance-sensitive electrodes is bonded to the glass substrate sputtered with gold interdigitated electrodes and the main body of the cell culture pool. One or more gold interdigitated electrodes are arranged on the glass substrate (3 ), the main parameters of each gold electrode are: 16 pairs of interdigitated electrodes, the width and interval of each pair of interdigitated electrodes are 100 μm, the length of the interdigitated fingers is 5 mm, the thickness of the gold layer is 200 nm, and the size of the pad is 2 mm × 2.5 mm; the cell culture pool The main body (i.e., the cell culture container) is cast from polydimethylsiloxane (PDMS), with a wall thickness of 1 mm. The inner edge of the contact surface with the glass substrate does not cover the interdigitated electrode pair, and the outer edge does not cover the electrode pin pad. The signal measured by the gold electrode is transmitted to the impedance detection equipment through the electrical connector (conductive slip ring). 2 passages for slip rings. The impedance detection equipment can be a commercial electrical impedance meter, an electrochemical workstation, or a self-built impedance acquisition and analysis module, which calculates the corresponding impedance value of the cells attached to the electrode based on the current or voltage signal conducted by the golden interdigitated electrode. After the relevant data is processed by the host computer data processing algorithm, the cell proliferation at different positions on the base of the cell culture container can be obtained, or the average cell proliferation on the base of the cell culture container can be obtained by taking the average value. Since the conductive slip ring can still transmit signals under the rotating state of the simulated weightless effect, the growth state of the cells under the simulated weightless effect can be detected online in real time through this method.

The working process is as follows: Place the closed cell culture container to be inoculated and sterilized on the ultra-clean workbench, use a 1ml sterile syringe to pierce the side wall of the bonding part between the main body of the cell culture container and the substrate of the closed cell culture container, and remove the The main body of the syringe, another 1ml sterile syringe draws the cell culture suspension to be inoculated and injects it into the airtight cell culture container through the side wall of the culture pool until there are no air bubbles in the pool, then unplug the two 1ml syringes; lead the electrical connector near the connecting plate end of the rotating shaft After welding to the interdigital electrode pads, fix the cell culture container on the rotating shaft connection plate of the main body of the rotary device; connect the other end of the electrical connector to the crocodile clip of the impedance detector, and connect the data transmission line of the impedance detector to the host computer. For the growth stage and the focus of attention, start the working software of the impedance detector, collect the cell impedance value at a fixed time interval (such as 30-60min), and use the host computer to process and analyze the value in real time.

The above is only a preferred embodiment of the utility model, it should be pointed out that for those of ordinary skill in the art, without departing from the principle of the utility model, some improvements and modifications can also be made, these improvements and Retouching should also be regarded as the scope of protection of the present utility model.

Claims (10)

1. A weightless simulation effect rotary device system capable of real-time online monitoring of cell growth status, characterized in that it comprises: a rotary device body for continuous cell rotation under controllable conditions; a cell culture container with impedance-sensitive electrodes, It is used to provide a carrier for cell adherent growth and sense impedance changes during cell culture. The cell culture container with impedance-sensitive electrodes is set in the body of the rotary device; it is used to detect, process and analyze cell impedance signals The peripheral detection and analysis module is connected to the body of the rotary device and the impedance-sensitive electrodes of the cell culture container respectively. 2. The weightless simulation effect rotary device system capable of real-time on-line monitoring of cell growth state according to claim 1, wherein the cell culture container comprises a fully-sealed cell culture tank to accommodate cells, and the cell culture tank's Several impedance-sensitive electrodes are arranged on the bottom, and the impedance-sensitive electrodes are connected to the pin pads, and the pin pads are connected to the impedance reading circuit of the peripheral detection and analysis module. 3. The weightless simulating effect rotary device system capable of real-time online monitoring of cell growth status according to claim 2, characterized in that the shape of the impedance-sensitive electrodes is interdigitated, ring-shaped or circular. 4. The weightless simulation effect rotary device system capable of real-time on-line monitoring of cell growth state according to claim 2, characterized in that the impedance sensitive electrode is directly processed on the inner wall of the bottom surface of the cell culture pool or embedded in the bottom surface of the culture container after being prepared on the glass substrate . 5. The weightless simulation effect rotary device system capable of real-time online monitoring of cell growth state according to claim 2, characterized in that the impedance-sensitive electrodes are connected to the pin pads, and the pin pads lead out wires by welding or clamping. The wire is connected to the impedance reading circuit of the peripheral detection and analysis module through the body of the rotary culture device. 6. The weightless simulating effect rotary device system capable of real-time online monitoring of cell growth status according to claim 2, characterized in that, the rotary device body includes a support end, a rotary transmission structure, a rotating shaft connecting plate, and a rotary condition control module , the supporting end is separated from both sides of the connecting plate of the rotating shaft, and there is a supporting hole on it, and the connecting plate of the rotating shaft is rotatably installed on the supporting end through the supporting hole of the supporting end, and the rotation condition control module includes The motor and the control assembly on the support end, the output shaft of the motor is connected to the connecting plate of the rotating shaft, and the cell culture container is installed on the connecting plate of the rotating shaft, so that the cell culture container and the connecting plate of the rotating shaft can rotate at the same speed, and the rotation condition control module The control assembly is used to set and adjust the rotation parameters of the connecting plate of the rotating shaft. The rotary transmission structure includes an electrical connector rotatably installed on the support end through the support hole. The pin pads are connected, and the back-end leads of the electrical connector are connected with the peripheral detection and analysis module. 7. The weightless simulation effect rotary device system capable of real-time online monitoring of cell growth status according to claim 6, wherein the peripheral detection and analysis module includes impedance detection equipment and a host computer, the impedance detection equipment is connected to the host computer, and the impedance detection The device is connected with the electrical connector for collecting changes in cell impedance, and the host computer is used to process the data of the impedance detection device and output analysis results on the screen of the host computer. 8. The weightless simulation effect rotary device system capable of real-time online monitoring of cell growth status according to claim 7, wherein the peripheral detection and analysis module also includes a time-sharing sequential gating module, which is connected to the Between the electrical connector and the impedance detection device, it is used for time-sharing sequential gating detection of the current or voltage of one or more impedance-sensitive electrodes. 9. The weightless simulation effect rotary device system capable of real-time on-line monitoring of cell growth status according to claim 8, wherein the impedance detection equipment is a commercial electrical impedance meter, an electrochemical workstation or an independently built impedance acquisition and analysis module. The calculation process is performed on the current and voltage values detected by each electrode in the same round, and the impedance-time curve corresponding to the average state of the cells or the state of the local area is output according to the requirements. 10. The weightless simulation effect rotary device system capable of real-time online monitoring of cell growth status according to claim 9, characterized in that the impedance detection equipment is an independently built impedance acquisition and analysis module, and the self-built impedance acquisition and analysis module includes data acquisition The chip, the digital lock-in amplifier, the display screen, and the data acquisition chip are connected with the time-sharing sequential gating module.

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