CN215627964U - Cell continuous rotary slewing device body for realizing controllable conditions - Google Patents
Cell continuous rotary slewing device body for realizing controllable conditions Download PDFInfo
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- CN215627964U CN215627964U CN202121130216.1U CN202121130216U CN215627964U CN 215627964 U CN215627964 U CN 215627964U CN 202121130216 U CN202121130216 U CN 202121130216U CN 215627964 U CN215627964 U CN 215627964U
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Abstract
The utility model provides a cell continuous rotation rotary device body for realizing controllable conditions, which comprises: the cell culture device comprises a support end, a rotary transmission structure, a rotary shaft connecting plate and a rotary condition control module, wherein the support end is separated from two sides of the rotary shaft connecting plate, the rotary shaft connecting plate is rotatably arranged on the support end through a support hole of the support end, the rotary condition control module comprises a motor and a control assembly which are arranged on the support end, an output shaft of the motor is connected with the rotary shaft connecting plate, and a cell culture container is arranged on the rotary shaft connecting plate so as to ensure that the cell culture container and the rotary shaft connecting plate rotate at the same speed. The utility model can monitor the cell growth state in real time, without standard and with high sensitivity, and provides monitoring technical guarantee for objective and accurate research of ground simulated weightlessness cell culture experiment; meanwhile, the method can also be used for monitoring the cell growth in the cell culture industry and experimental research based on the gyrator in the fields of biotechnology, medical diagnosis and the like.
Description
Technical Field
The utility model relates to a biological monitoring device, in particular to a cell continuous rotation rotating device body for realizing controllable conditions.
Background
The cell weightlessness simulation effect rotation device is an important basic platform for cell culture research under the weightlessness environment on the ground, however, the existing weightlessness simulation effect rotation device has no function of monitoring the cell growth state on line in real time, and cannot capture the cell change in the rotation process, so that important hidden information may be lost. Conventional cell proliferation detection means such as microscope imaging, fluorescence labeling detection, flow cytometry and the like cannot realize real-time monitoring of cell growth in a rotation process, or cannot realize quantitative detection, or needs special treatment on cells, or needs to take out cells to terminate culture.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a cell continuous rotation rotary device body for realizing controllable conditions.
The utility model relates to a cell continuous rotation device body for realizing controllable conditions, which comprises a support end, a rotation transmission structure, a rotation shaft connecting plate block and a rotation condition control module, wherein the support end is separated from two sides of a rotation shaft connecting plate block and is provided with a support hole, the rotation shaft connecting plate block is rotatably arranged on the support end through the support hole of the support end, the rotation condition control module comprises a motor and a control assembly which are arranged on the support end, an output shaft of the motor is connected with the rotation shaft connecting plate block, a cell culture container is arranged on the rotation shaft connecting plate block so as to ensure that the cell culture container and the rotation shaft connecting plate block rotate at the same speed, the control assembly of the rotation condition control module is used for setting and adjusting the rotation parameters of the rotation shaft connecting plate block, the rotation transmission structure comprises an electric connector which is rotatably arranged on the support end through the support hole, the front end lead of the electric connector is connected with the pin bonding pad of the cell culture container, and the rear end lead of the electric connector is connected with the peripheral detection analysis module.
The cell continuous rotation slewing device body for realizing controllable conditions comprises a peripheral detection analysis module, an upper computer and a control module, wherein the peripheral detection analysis module comprises impedance detection equipment and the upper computer, the impedance detection equipment is connected with an electric connector and used for collecting cell impedance changes, and the upper computer is used for processing data of the impedance detection equipment and outputting analysis results on a screen of the upper computer.
The cell continuous rotation slewing device body for realizing controllable conditions further comprises a time-sharing sequential gating module, wherein the time-sharing sequential gating module is connected between the electric connector and the impedance detection equipment and is used for time-sharing sequential gating detection of current or voltage of one or more impedance sensitive electrodes.
The cell continuous rotation slewing device body for realizing condition controllable is characterized in that the impedance detection equipment is a commercial electrical impedance instrument, an electrochemical workstation or an independently constructed impedance acquisition and analysis module, is used for carrying out operation processing on a current voltage value detected by each electrode in the same round, and outputs an impedance-time curve corresponding to a cell average state or a local area state according to requirements.
The cell continuous rotation slewing device body for realizing controllable conditions is characterized in that the impedance detection equipment is an independently constructed impedance acquisition and analysis module, the independently constructed impedance acquisition and analysis module comprises a data acquisition chip, a digital phase-locked amplifier and a display screen, and the data acquisition chip is connected with the time-sharing sequential gating module.
The cell continuous rotation slewing device body for realizing condition controllable, which is disclosed by the utility model, is oriented to the field of aerospace medical experiments, can monitor the cell growth state in real time, without a standard and with high sensitivity, and provides a monitoring technical guarantee for objective and accurate research of ground simulated weightless cell culture experiments. Meanwhile, the continuous cell rotation slewing device body for realizing controllable conditions can be used for monitoring the growth of cells in the cell culture industry and experimental research based on the slewing device in the fields of biotechnology, medical diagnosis and the like.
Drawings
FIG. 1 is a schematic structural diagram of a turning device body according to the present invention;
FIG. 2 is a schematic structural diagram of a weightlessness simulation effect rotating device system capable of monitoring the growth state of cells on line in real time;
FIG. 3 is a schematic structural diagram of a cell culture container of a weightlessness simulation effect rotating device system capable of monitoring the growth state of cells on line in real time.
Detailed Description
As shown in fig. 1, the continuous cell rotation device body for realizing controllable conditions comprises a support end 21, a rotation transmission structure, a rotation shaft connecting plate 23 and a rotation condition control module 24, wherein the support end is separated from two sides of the rotation shaft connecting plate and is provided with a support hole, the rotation shaft connecting plate is rotatably arranged on the support end through the support hole of the support end, the rotation condition control module comprises a motor and a control component, the motor is arranged on the support end, an output shaft of the motor is connected with the rotation shaft connecting plate, a cell culture container is arranged on the rotation shaft connecting plate to enable the cell culture container and the rotation shaft connecting plate to rotate at the same speed, the control component of the rotation condition control module is used for setting and adjusting the rotation parameters of the rotation shaft connecting plate, the rotation transmission structure comprises an electric connector 25 rotatably arranged on the support end through the support hole, the front end lead of the electric connector is connected with the pin bonding pad of the cell culture container, and the rear end lead of the electric connector is connected with the peripheral detection analysis module. The peripheral detection analysis module comprises impedance detection equipment 32 and an upper computer 33, the impedance detection equipment is connected with the upper computer, the impedance detection equipment is connected with the electric connector and used for collecting cell impedance changes, and the upper computer is used for processing data of the impedance detection equipment and outputting analysis results on a screen of the upper computer. The peripheral detection analysis module further comprises a time-sharing sequential gating module, wherein the time-sharing sequential gating module is connected between the electric connector and the impedance detection equipment and is used for gating and detecting the current or the voltage of one or more impedance sensitive electrodes in a time-sharing sequential manner. The impedance detection equipment is a commercial electrical impedance instrument, an electrochemical workstation or an independently constructed impedance acquisition and analysis module, and is used for carrying out operation processing on the current and voltage values detected by each electrode in the same turn and outputting an impedance-time curve corresponding to the average state or the local area state of the cell according to the requirement. The impedance detection equipment is an independently constructed impedance acquisition and analysis module which comprises a data acquisition chip, a digital phase-locked amplifier and a display screen, wherein the data acquisition chip is connected with the time-sharing sequential gating module.
As shown in fig. 2 and 3, a weightlessness simulation effect rotation device system capable of monitoring the growth state of cells on line in real time comprises: the cell culture container 1 is provided with an impedance sensitive electrode and is used for providing a carrier for the adherent growth of cells and sensing the impedance change in the cell culture process; the rotating device body is used for realizing the continuous rotation of the cells with controllable conditions, and the cell culture container with the impedance sensitive electrode is arranged in the rotating device body; and the peripheral detection and analysis module is used for realizing the detection, processing and analysis of cell impedance signals and is respectively connected with the impedance sensitive electrodes of the rotating device body and the cell culture container.
The cell culture container comprises a fully-closed cell culture pool for accommodating cells, a plurality of same or different impedance sensitive electrodes 11 are arranged on the inner wall of the bottom of the cell culture container 1, the impedance sensitive electrodes are connected with pin pads 12, and the pin pads are connected with an impedance reading circuit of the peripheral detection and analysis module. The cell culture container comprises a closed cell culture pool for accommodating cells, a plurality of impedance sensitive electrodes are arranged at the bottom of the cell culture pool, the impedance sensitive electrodes are connected with pin bonding pads, and the pin bonding pads are connected with an impedance reading circuit of the peripheral detection and analysis module. The cell culture container is totally closed, so that the cell culture container can be used for cell culture in any posture (such as static flat placement, static inclination and continuous rotation). The impedance sensitive electrode is in the shape of an interdigital, a ring or a circle. The shape of the impedance sensitive electrode is interdigital, annular, circular or other special shapes with detection sensitivity/resolution meeting the test requirement. The impedance sensitive electrode is directly processed on the inner wall of the bottom surface of the cell culture pool or is embedded into the bottom surface of the culture container after being prepared on a glass substrate. The impedance sensitive electrode is connected with a pin bonding pad, a lead is led out from the pin bonding pad in a welding or clamping mode, and the lead is connected to an impedance reading circuit of the peripheral detection analysis module through the rotary culture device body.
The utility model can be applied to the research fields of medicine, life science, cell biology and the like, integrates a detection system based on an electrical impedance spectroscopy and has the function of monitoring the cell state on line in real time. Can realize on-line detection and continuous monitoring without marking, non-invasion and influence on normal growth of cells.
The specific application embodiment of the weightlessness simulation effect rotating device system capable of monitoring the cell growth state in real time on line is as follows:
the cell culture container with the impedance sensitive electrode is formed by bonding a glass substrate sputtered with gold interdigital electrodes and a cell culture pool main body, wherein 1 or more gold interdigital electrodes (3 gold interdigital electrodes are arranged in the embodiment) are arranged on the glass substrate, and the main parameters of each gold electrode are as follows: 16 pairs of interdigital electrodes, wherein the width and the interval of each pair of interdigital electrodes are 100 mu m, the length of the interdigital is 5mm, the thickness of the gold layer is 200nm, and the size of the bonding pad is 2mm multiplied by 2.5 mm; the cell culture pond main body (namely a cell culture container) is formed by pouring Polydimethylsiloxane (PDMS), the wall thickness is 1mm, the inner edge of the contact surface of the cell culture pond main body and the glass substrate does not cover the interdigital electrode pair, and the outer edge of the contact surface of the cell culture pond main body and the glass substrate does not cover the electrode pin bonding pad. And signals detected by the gold electrodes are transmitted to the impedance detection equipment through an electric connector (conductive slip ring), the number of the conductive slip rings corresponds to the number of the interdigital electrodes, and 2 paths of the conductive slip rings are respectively welded on 2 bonding pads of each interdigital electrode. The impedance detection equipment can be a commercial electrical impedance instrument, an electrochemical workstation or an autonomously constructed impedance acquisition and analysis module, and calculates an impedance value corresponding to cells adhered to the electrodes according to current or voltage signals conducted by the gold interdigital electrodes. After the relevant data is processed by the upper computer data processing algorithm, the cell proliferation conditions at different positions on the cell culture container substrate can be obtained, or the average cell proliferation condition on the cell culture container substrate can be obtained by taking an average value. The conductive slip ring can still transmit signals under the condition of weightlessness simulation effect rotation, so that the method can carry out real-time online detection on the growth state of cells under the simulated weightlessness effect.
The working process is as follows: placing the closed cell culture container to be inoculated with cells and sterilized in a super-clean workbench, pricking a part of side wall of a bonding part of a culture tank main body and a substrate of the closed cell culture container by using a 1ml sterile injector, removing the injector main body, sucking a cell culture suspension to be inoculated by using another 1ml sterile injector, injecting the cell culture suspension into the closed cell culture container through the side wall of the culture tank until no bubbles exist in the culture tank, and pulling out the two 1ml injectors; welding a lead at one end of the electric connector close to the rotating shaft connecting plate to an interdigital electrode pad, and fixing the cell culture container on the rotating shaft connecting plate of the main body of the rotating device; and connecting a lead at the other end of the electric connector to an alligator clip of the impedance detector, connecting a data transmission line of the impedance detector to an upper computer, starting working software of the impedance detector according to the growth stage and the focus of the cells, collecting the impedance value of the cells at fixed time intervals (such as 30-60min), and carrying out real-time processing and analysis on the value by using the upper computer.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (5)
1. A cell continuous rotation device body for realizing condition controllable is characterized in that the rotation device body comprises a support end, a rotation transmission structure, a rotation shaft connecting plate block and a rotation condition control module, wherein the support end is separated from two sides of the rotation shaft connecting plate block and is provided with a support hole, the rotation shaft connecting plate block is rotatably arranged on the support end through the support hole of the support end, the rotation condition control module comprises a motor and a control component which are arranged on the support end, an output shaft of the motor is connected with the rotation shaft connecting plate block, a cell culture container is arranged on the rotation shaft connecting plate block to enable the cell culture container and the rotation shaft connecting plate block to rotate at the same speed, the control component of the rotation condition control module is used for setting and adjusting rotation parameters of the rotation shaft connecting plate block, the rotation transmission structure comprises an electric connector which is rotatably arranged on the support end through the support hole, the front end lead of the electric connector is connected with the pin bonding pad of the cell culture container, and the rear end lead of the electric connector is connected with the peripheral detection analysis module.
2. The continuous cell rotation rotary device body for realizing controllable conditions according to claim 1, wherein the peripheral detection and analysis module comprises an impedance detection device and an upper computer, the impedance detection device is connected with the electric connector and is used for collecting cell impedance changes, and the upper computer is used for processing data of the impedance detection device and outputting analysis results on a screen of the upper computer.
3. The device body for realizing continuous cell rotation under controllable conditions as claimed in claim 2, wherein the peripheral detection and analysis module further comprises a time-sharing sequential gating module, the time-sharing sequential gating module is connected between the electrical connector and the impedance detection device and is used for time-sharing sequential gating detection of the current or voltage of the one or more impedance sensitive electrodes.
4. The continuous cell rotation and gyration device body for realizing controllable conditions according to claim 3 is characterized in that the impedance detection equipment is a commercial electrical impedance instrument, an electrochemical workstation or an independently constructed impedance acquisition and analysis module, and is used for performing operation processing on the current and voltage values detected by each electrode in the same turn and outputting an impedance-time curve corresponding to the average state or the local area state of the cell according to the requirement.
5. The cell continuous rotation rotary device body for realizing controllable conditions according to claim 4, wherein the impedance detection equipment is an independently constructed impedance acquisition and analysis module, the independently constructed impedance acquisition and analysis module comprises a data acquisition chip, a digital phase-locked amplifier and a display screen, and the data acquisition chip is connected with the time-sharing sequential gating module.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121130216.1U CN215627964U (en) | 2021-05-25 | 2021-05-25 | Cell continuous rotary slewing device body for realizing controllable conditions |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202121130216.1U CN215627964U (en) | 2021-05-25 | 2021-05-25 | Cell continuous rotary slewing device body for realizing controllable conditions |
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| CN215627964U true CN215627964U (en) | 2022-01-25 |
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| CN202121130216.1U Active CN215627964U (en) | 2021-05-25 | 2021-05-25 | Cell continuous rotary slewing device body for realizing controllable conditions |
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2021
- 2021-05-25 CN CN202121130216.1U patent/CN215627964U/en active Active
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